JPH02143162A - Bioexperimenting device - Google Patents

Abstract

PURPOSE:To obtain the device which effectively experiments the photoreaction of a life body by changing a color temp. or light energy or maintaining the color temp. and/or the light energy constant and irradiating the life body with light. CONSTITUTION:Light from which UV rays and IR rays are removed is introduced in an optical cable 1. The life body 4 is housed into a Petri dish 3. The life body is irradiated with the light from the cable through a light transparent part 5a of a cap 5 and the photoreaction is observed. The surface 5b exclusive of the light transparent part 5a acts as a reflection surface to prevent the heating up of the cap 5; further, the light transmitted through the Petri dish 3 is reflected by a mirror 6 to prevent the heating up of a Petri dish supporting plate 2. The sample 4 is irradiated with the optimum light energy by a lux meter 8 and the wavelength component of the light is adjusted by a color thermometer 9 so that the sample is irradiated with the light of the optimum color temp. The illuminance change is executed by moving the cable 1 in an arrow direction and the color temp. change is executed by changing the wavelength component of the light introduced into the cable 1. The bioexperiment of the life body is executed with high accuracy by the irradiation of the visible light according to this constitution.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an experimental device for examining living organisms by irradiating visible light components with light.

The present applicant first focuses sunlight or artificial light with a lens or the like and introduces it into a light guide, and then transmits it to any desired location through the light guide for illumination or other purposes, such as cultivating plants. , proposed the use of chlorella in cultivation, etc., but in the process, visible light that does not include ultraviolet rays or infrared rays promotes biological reactions and improves health, or prevents skin aging. are gout, neuralgia, bedsores, and rheumatism.

It has been discovered that it is effective in recovering from burns, skin diseases, (If! scars, fracture scars, etc.) and as a pain reliever.

Furthermore, in view of the above-mentioned circumstances, the present applicant

Previously, we have proposed a light irradiation treatment device that can perform various treatments or improve beauty or health by irradiating light corresponding to the visible light component of sunlight that does not contain harmful components such as ultraviolet rays and infrared rays. This device irradiates the patient's skin surface with light from the visible light component of sunlight or artificial light introduced into a photoconductor cable. According to this device, the treatment is effective without suffering from the harmful effects of ultraviolet rays or infrared rays. is obtained. Furthermore, in order to make the therapeutic effect of this device more effective, the light amount and light wavelength components are adjusted.

It is required to investigate the synergistic effect of medicinal effects under light irradiation by varying the intensity of light, etc., but this investigation is conducted through animal experiments such as rabbits and rats.

As mentioned above, the present applicant has previously proposed various ways to use the visible light component of sunlight to activate living organisms. The purpose was to provide a device for more effectively experimenting with the body's photoreactions.

1-11 Figure 1 is a configuration diagram for explaining an embodiment of the bioexperimental apparatus according to the present invention.In Figure 1, 1 is a light guide cable;
Light corresponding to the visible light component (white light) of sunlight or artificial light, or light containing a large amount of red, blue, or green components is transmitted through the guide cable 1 (however.

2 is a transparent substrate, 3 is a petri dish made of transparent material, and 4 is a test object placed in the petri dish 3 (infrared rays and ultraviolet rays that have a negative effect on living organisms are removed). 5 is a lid plate placed on the petri dish 4, a part of the lid member 4, for example, a central part 5a, is formed transparent or hollow, and a surface 5b of the peripheral part is formed as a reflective surface. has been done.

Reference numeral 6 denotes a reflecting mirror arranged at an angle below the base 2.

The light emitted from the optical conductor cable 1 is irradiated onto the living organisms in the Petri dish 3 through the transparent portion 5a of the lid member 5, and the photoreaction of the living organisms is observed. However, at that time,
When something other than light, such as heat, acts on a living organism, it becomes impossible to distinguish between the organism's light reaction and thermal reaction, making it impossible to conduct accurate experiments.

The present invention has been made with the aim of eliminating such thermal reactions as much as possible, and as shown in the figure, the cover member 5 other than the transparent part 5a is made into a reflective surface 5b, thereby preventing radiation from the optical conductor cable 1. The increase in temperature of the lid member 5 due to the emitted light is kept as small as possible, and furthermore, the light transmitted through the petri dish 3 is reflected by the reflecting mirror 6 to suppress the increase in temperature of the substrate 2. In addition, although the light-transmitting part 5a may be hollow (hole) as shown in the figure, it may also be a transparent member integral with the lid member 5.

Figure 2 is a view of the board 2 from the back side, as shown in Figure 1.
The light reflected by the reflecting mirror 6 should be prevented from hitting the base #7 of the substrate 2, and the light reflected by the reflector 6 should be prevented from hitting the substrate 2 and, by extension, the petri dish 3.
Heat is not applied to the test object 4 inside.

In addition, 8 is a light energy sensor (illuminance meter), and 9 is a color temperature meter, which detects the intensity of the light irradiated to the test object 4 by the light energy sensor 8, and always supplies the optimum light energy to the test object 4. In addition, the color temperature of the light irradiated onto the test object 4 by the color thermometer 9, that is, the wavelength component of the light irradiated onto the test object 4, is adjusted so that it is always optimal for the test object 4. It emits light with a certain color temperature. That is, the test object 4 is exposed to light irradiated by the test object.

It has optimal illuminance and color temperature, and in order to conduct experiments effectively, it is necessary to optimize these illuminance and color temperature according to the test object, and it is necessary to change the illuminance (intensity of light energy). To do this, the emission end of the optical conductor cable 1 may be moved in the direction indicated by the arrow, and to change the color temperature, the wavelength component of the light introduced into the optical conductor cable 1 may be changed.

FIG. 3 is a diagram showing an example of a device for adjusting illuminance and color temperature as described above, and in the figure, 10 is a substrate (which can be considered to correspond to the cover plate 5 shown in FIG. 1). , 11 is a column erected on the substrate 10, 12 is a fixed plate provided at the upper end of the column 10, 13 is a movable plate that moves up and down along the column 10, and 14 is the movable plate 13 indicated by an arrow. A motor for driving in direction B; 15 is a screw rod rotated by the motor 14; 16 is a nut member screwed onto the screw 15; the nut member 16 is integrally attached to the movable plate 13; ing. Therefore, in this configuration, two motors 14
When the threaded rod 15 is rotated, the nut member 16 is fixed to the movable plate 13 and its rotation is prevented, so the movable plate 13 moves in the direction of arrow B in accordance with the rotation of the screw stud 15.

17 is a light guide cable fixedly attached to the center of the movable plate 13, and 18a, 18b, 18c (18c is not shown) are movable on the movable plate 13 in the direction of arrow C as shown in the figure. Each of the other light guide cables 19a, 19b, 19c (
19e (not shown) are light guide cables 18, respectively.
a.

Support arm 20a supporting 18b, 18c.

20b, 20c (20c not shown) are arms for moving the movable arms 19a, 19b, 19c in the direction of arrow C and rotating in the direction of arrow θ; 21a;
21b and 21c (21c is not shown) are the arms 20
a, 19a;, 20b.

19 b ; , 20 c +' 19 c as arrow θ
Motors for rotating in the directions, 22a, 22b, 22c
(22C not shown) are motors 21a and 2, respectively.
The telescoping arms 23a, 23b, 23c (23c not shown), whose lengths change in the direction of the arrow by the rotation of 1b, 21c, are each threaded studs 24a.

24b, 24c (24b, 24c not shown), 25a, 25b, 25c (25b, 25
(c not shown) are screw studs 24a, 24b, 2, respectively.
The light emitted from the light conductor cable 17 provided at the center with a nut screwed into the light guide cable 18a corresponds to, for example, the white light of sunlight, and the light emitted from the light conductor cable 18a has a red component. It is light that contains a large amount of light equivalent to
The light emitted from 18b contains a large amount of light corresponding to a blue component, and the light emitted from 18c contains a large amount of light corresponding to a green component. Therefore, by adjusting the amount of light emitted from the optical conductor cables 18a, 18c, and 18d, the combined light emitted from these optical conductor cables can be made into white light, light with a large red component, or blue light. Various changes can be made, such as using light with many components or light with many green components. That is, in the device shown in FIG.
The light emitted from 8a to 18c is transmitted through the transparent portion 1 of the substrate 10.
The irradiated object 28 is irradiated through 0a, but at that time,
The light from each optical conductor cable is combined near the irradiated object 28, and this combined light is transmitted to the irradiated object 28.
The illuminance and color temperature are adjusted to the most appropriate level. However, if the object to be irradiated 28 is changed to another object or the experimental conditions are changed, it is necessary to change the illuminance or the color temperature. In addition, even if you want to keep the illumination intensity and color temperature constant for the same irradiated object, the energy of the light transmitted through the optical conductor cable may change depending on the weather or the time of day, and the color intensity may change. In terms of color temperature, red components increase in the morning and evening.

Figure 3 shows an example of a device that can deal with such cases. l! I! ! To do this, the motor 14 is rotated to move the movable plate 13 in the direction of arrow B. However, in that case, the light conductor cables 18a, 1
8b and 18c also move up and down together with the movable plate 13, so if the movable plate 13 is simply moved up and down, the combination of light emitted from these optical conductor cables will be transferred to a location other than the irradiated object 28. , 21b, 21c and the movable arms 22a, 22b, 22c are provided to eliminate such problems.When the movable plate 13 is moved up and down as described above, the motor 21 is
a, 21b, 21c to drive the arms 22a, 22b,
The motor 21a (21b,
21c) and the arm 2 rotated by the screw foot 21a'
A nut 22a fixed to the motor 21a (21b, 22c) is screwed onto the motor 21a (21b).

21c) to move the arm 22a in the direction of arrow C.
For example, when moving the movable plate 13 upward, the arm 22a is pulled toward the motor 21 side, and when moving the movable plate 13 downward, the arm 22a is pulled away from the motor 21a. If you move it away, it will be arm 24a.

19a (24b, 19b; the same applies to 24c, 19c) rotates in the θ direction about the fulcrum 26a to irradiate the irradiated object 28 with the light emitted from these optical conductor cables 18a, 18b, 18c. can. In other words, without changing the color temperature.

Light can be applied to the irradiated object. In addition, the irradiated object 2
To adjust the color temperature of the light irradiated to the motor 23
a (the same applies to 23b and 23c) and rotates the screw pedestal 24a, the nut 25a, which is screwed to the screw pedestal 24 and is prevented from rotating, moves along the screw pedestal 24a, and thus , the support arm 19a supporting the optical conductor cable 18a moves in the direction of arrow C, thereby adjusting the color temperature.6 For example, if you want the chromaticity to be close to red, the red component light is The light conductor cable 1.8a, which is included in a large amount, should be brought closer to the irradiated object 28, and the other light guide cables 18b and 18C should be moved away, and these light guide cables L8a to 18c can reach the irradiated object 28. By adjusting the length of , it is possible to irradiate the irradiated object 28 with light of a desired color temperature. 2
Reference numeral 7 denotes a height adjustment tool that adjusts the height of the substrate 10 in an IJIW manner, thereby adjusting the height position of the substrate 10 so that light can suitably hit the irradiated object 28 disposed below the substrate 10. That's what I do. Although FIG. 3 shows the use of animals as objects to be irradiated, this is not limited to animals, but includes humans, plants, and fish.

Any desired living body, such as a cell, can be used as the irradiated object2.
For example, it is also possible to conduct an experiment by placing a Petri dish as shown in FIG. 1 under the substrate 1o.

FIG. 4 is a block diagram for explaining an example of a sunlight collecting device for introducing sunlight into the optical conductor cable 1. In the figure, 30 is a transparent protective capsule, 31 is a Fresnel lens, 32 is a lens holder, 33 is a sunlight direction sensor, 34 is an optical fiber whose light-receiving end is arranged at the focal position of a Fresnel lens, or an optical fiber cable consisting of multiple optical fibers (hereinafter referred to as a light guide), 35 is an optical fiber or optical fiber. The fiber cable holder 36 is an arm, 37 is a pulse motor, and 38 is a horizontal rotation shaft rotated by the pulse motor 37.

39 is a base for mounting the protective capsule 30; 4
0 is a pulse motor, and 41 is a vertical rotation shaft rotated by the pulse motor 40. The sunlight collecting device detects the direction of the sun with the sunlight direction sensor 33, and uses the detection signal to detect the direction of the sun. The pulse motors 37 and 40 of the horizontal rotation axis 38 and the vertical rotation axis 41 are respectively driven so that the rotation axis 33 faces toward the sun, so that the sunlight focused by each lens 31 is received at the focal position of each lens. The end is adapted to be introduced into the disposed light guide 34. The nine light guides 34 arranged for each lens are bundled together and led out of the solar light collection device as a light guide cable 1 and guided to any desired location, as described above.

Used for photoreaction experiments on living organisms.

FIG. 5 is a diagram for explaining details for introducing sunlight collected by the lens 31 into the light guide. In FIG. 2, 31 is a lens such as a Fresnel lens, and 34 is a lens 3.
This is a light guide for introducing sunlight focused by 1 and transmitting the introduced sunlight to any desired location.When the sunlight is focused by a lens system, the solar image is As shown in the figure, the center A becomes almost white light,
The peripheral area B contains many light components with wavelengths matching the focal position. In other words, when sunlight is focused by a lens system, the focal position and the size of the solar image differ depending on the wavelength of the light. For example, for blue light with a short wavelength, a solar image with a diameter of D is placed at the position of P□. , the green light beams at the position P2 and forms the image of the sun, and the red light beam forms the sun image at the position P. Therefore, in the case shown, the light beams at the position Pl. If the light-receiving end face of the conductor is placed,
Sunlight that contains a lot of blue component light in the periphery can be collected, and if placed at position P2, sunlight that contains a lot of green light component in the periphery can be collected, and if placed at position P3. If you do this, you can collect sunlight that contains a lot of red light components in the peripheral area, and in that case, adjust the diameter of the light guide to match the light component you want to collect.For example, if it is blue light, D.
By setting L for green type and D for red type, the amount of light guide used can be reduced and sunlight containing a large amount of the desired light component can be collected most efficiently. .

Furthermore, as shown in Figure 1, it is also possible to increase the diameter of the light-receiving end face of the light guide 34 so as to collect light containing all wavelength components. In addition, when aligning the light-receiving end surface of the light guide 34 with the focal position of the lens system, in advance,
The socket end face may be fixed at the focal position at the production factory, or the socket end face of the light guide may be made adjustable in the optical axis direction of the lens system, and the user can adjust the light of the desired color. Although it may be adjusted and fixed at the same time, in the case of the embodiment shown in FIG.

As is clear from the above description, according to the present invention, light can be applied to living organisms by varying the intensity and light energy of one color, or by keeping the color temperature and/or light energy constant. This allows for more accurate and efficient experiments on the photoreactions of living organisms.

[Brief explanation of the drawing]

FIG. 1 is a configuration diagram for explaining one embodiment of the bioexperimental apparatus according to the present invention, FIG. 2 is a back view of FIG. 1, and FIG.
The figure shows an example of a light irradiation device suitable for use in the experimental equipment shown in Figure 1, and Figure 4 shows an automatic sunlight collection and transmission device that introduces sunlight into a light conductor cable and transmits it. A diagram showing an example; FIG. 5 is a diagram of the principle for introducing light of a desired color component into a light conductor cable; FIG. 6 is a diagram showing an example of a solar image formed by a lens. . DESCRIPTION OF SYMBOLS 1... Photoconductor cable 52... Board, 3... Petri dish, 4... Test object (living body), 5... Cover plate, 6...
·Reflector. 8...Luminance meter, 9...Color temperature meter. Figure 1 Figure 2 Figure 2

Claims (1)

[Claims] 1. A base plate made of a transparent body, and a lid plate having a transparent part in the center and a reflective surface formed around the transparent part, and on the base plate. A bioexperimental apparatus characterized in that a petri dish is placed on the petri dish, the lid plate is placed on top of the petri dish, and light is irradiated to the test object in the petri dish through the light-transmitting part of the lid plate. 2. The lid plate has an illuminance sensor and/or a color temperature sensor on the surface thereof, so that the illuminance and/or color temperature of the light irradiated onto the test object in the petri dish can be monitored and/or adjusted. The bioexperimental apparatus according to claim 1. 3. The light irradiated onto the test object in the petri dish is the light transmitted through the optical conductor cable, and by changing the distance between the light emitting end of the optical conductor cable and the test object, it can be applied to the test object. 3. The bioexperimental apparatus according to claim 2, wherein the illuminance of the irradiated light is adjusted. 4. It has at least three optical conductor cables, and one of them emits light containing a large amount of red component light to the other one.
One book emits light containing a large amount of blue component light, and the remaining one emits light containing a large amount of green component light, and the distance from the light emitting end of the light conductor cable to the test object is adjusted respectively. 4. The bioexperimental apparatus according to claim 2, wherein the color temperature of the light irradiated onto the test object is changed by changing the color temperature of the light irradiated onto the test object.