JPH02138962A - Device for cell culture - Google Patents
Device for cell cultureInfo
- Publication number
- JPH02138962A JPH02138962A JP29263388A JP29263388A JPH02138962A JP H02138962 A JPH02138962 A JP H02138962A JP 29263388 A JP29263388 A JP 29263388A JP 29263388 A JP29263388 A JP 29263388A JP H02138962 A JPH02138962 A JP H02138962A
- Authority
- JP
- Japan
- Prior art keywords
- tank
- cell culture
- culture
- section
- separation membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 48
- 239000012528 membrane Substances 0.000 claims abstract description 28
- 239000002699 waste material Substances 0.000 claims abstract description 28
- 239000001963 growth medium Substances 0.000 claims abstract description 26
- 239000003102 growth factor Substances 0.000 claims abstract description 14
- 235000015097 nutrients Nutrition 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 11
- 238000011084 recovery Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 abstract description 18
- 210000002966 serum Anatomy 0.000 abstract description 7
- 238000000605 extraction Methods 0.000 abstract description 5
- 238000012258 culturing Methods 0.000 abstract description 4
- 238000012423 maintenance Methods 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 238000007599 discharging Methods 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract 3
- 239000004615 ingredient Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 14
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 9
- 239000001301 oxygen Substances 0.000 description 9
- 229910052760 oxygen Inorganic materials 0.000 description 9
- 239000012737 fresh medium Substances 0.000 description 8
- 239000007789 gas Substances 0.000 description 8
- 239000002609 medium Substances 0.000 description 7
- 238000001514 detection method Methods 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000003750 conditioning effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は細胞を培養する装置に関し、特に温度、ｐ、
Ｈ５溶存酸素濃度（Ｄ○）を適正に保ち、栄養分供給と
老廃物除去のために培養液を自動的に交換する細胞培養
装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to an apparatus for culturing cells, and in particular, to
This invention relates to a cell culture device that maintains an appropriate H5 dissolved oxygen concentration (D◯) and automatically exchanges the culture medium for the purpose of supplying nutrients and removing waste products.
このような細胞培養装置は、例えば細胞を高密度に培養
する高密度細胞培養装置として利用される。Such a cell culture device is used, for example, as a high-density cell culture device for culturing cells at high density.
（従来の技術）
高密度細胞培養装置では細胞を含み細胞培養槽に収容さ
れた培養液の上面にガスが供給されて接触する。培養液
にはｐＨセンサ、溶存酸素濃度センサ及び温度センサが
浸され、液面レベルを検出するレベルセンサが設けられ
ており、ｐＨセンサの検出信号によって培養液上面への
ＣＯ２ガス供給量が制御され、溶存酸素濃度センサの検
出信号によって培養液上面への０２ガス供給量が制御さ
れ、温度センサの検出信号によって培養液を加熱するヒ
ータへの通電が制御され、培養液の一部が取り出された
ときレベルセンサの検出信号によって新鮮培地供給用の
ポンプが作動して新鮮培地が細胞培養槽に供給されて細
胞培養槽内の培養液量が一定に保たれる。(Prior Art) In a high-density cell culture device, a gas is supplied to and comes into contact with the upper surface of a culture solution containing cells and housed in a cell culture tank. A pH sensor, a dissolved oxygen concentration sensor, and a temperature sensor are immersed in the culture solution, and a level sensor is provided to detect the liquid level, and the amount of CO2 gas supplied to the top of the culture solution is controlled by the detection signal of the pH sensor. The amount of 02 gas supplied to the top surface of the culture medium was controlled by the detection signal of the dissolved oxygen concentration sensor, the energization to the heater that heated the culture medium was controlled by the detection signal of the temperature sensor, and a portion of the culture medium was taken out. At this time, a fresh medium supply pump is activated by the detection signal of the level sensor, and fresh medium is supplied to the cell culture tank, so that the amount of culture solution in the cell culture tank is kept constant.
培養液の一部を取り出すために、例えば細胞培養槽に培
養液を槽外に循環させる循環部が設けられ、循環部に例
えばセラミック製の円筒状のクロスフローフィルタが設
けられ、細胞を含む培養液はそのフィルタの内部を通過
し、そのフィルタから注出された液が排出される。In order to take out a part of the culture solution, for example, a cell culture tank is provided with a circulation part that circulates the culture solution outside the tank, and a cylindrical cross-flow filter made of ceramic, for example, is provided in the circulation part, and the cell culture tank is equipped with a cylindrical cross-flow filter made of ceramic. The liquid passes through the interior of the filter, and the liquid poured out from the filter is drained.
（発明が解決しようとする課題）
従来の装置において培養液の一部を取り出すためのフィ
ルタにおける分離は、細胞と細胞上清を分離するだけで
あり、細胞上清中に含まれる高分子蛋白や血清成分、特
に成長因子（ＧＦ）も−緒に系外へ排出されてしまう。(Problems to be Solved by the Invention) In conventional devices, the separation using a filter to take out a part of the culture solution only separates cells and cell supernatant, and the high molecular weight proteins and other substances contained in the cell supernatant are separated. Serum components, especially growth factors (GF), are also excreted out of the system.
血清やＧＦは高価であるため維持費が高くつく。Since serum and GF are expensive, maintenance costs are high.
また、老廃物を選択的に排出することができない。Furthermore, waste products cannot be selectively excreted.
本発明は成長因子や栄養分を系外に出さずに老廃物を選
択的に排出することにより、維持費を下げることのでき
る細胞培養装置を提供することを目的とするものである
。An object of the present invention is to provide a cell culture device that can reduce maintenance costs by selectively discharging waste products without leaving growth factors or nutrients out of the system.
（課題を解決するための手段）
本発明の細胞培養装置は、細胞を培養する細胞培養槽の
他に調整槽、交換部及び回収部を備えている。(Means for Solving the Problems) The cell culture device of the present invention includes, in addition to a cell culture tank for culturing cells, an adjustment tank, an exchange section, and a collection section.
細胞培養装置はｐＨ１溶存酸素濃度及び温度などの培養
条件を制御する手段、細胞を植え込んだり採取したりす
るポート並びに培養液を槽外に循環させる第１の循環部
を備えている。調整槽は培養液を槽”外に循環させる第
２の循環部及び液量に応じて新鮮培地を流入させる手段
を備えている。The cell culture device includes means for controlling culture conditions such as pH1 dissolved oxygen concentration and temperature, a port for implanting and harvesting cells, and a first circulation section for circulating the culture solution outside the tank. The adjustment tank is equipped with a second circulation section that circulates the culture solution to the outside of the tank, and means that allows fresh culture medium to flow in according to the amount of the solution.
交換部は第１の循環部と第２の循環部の間に設けられて
細胞及び成長因子を通さず栄養分及び老廃物を通す粗さ
の膜を備えている。回収部は第２の循環部の流路で前記
交換部より下流側に設けられて栄養分を通さず老廃物を
通す粗さの膜を備えその膜から抽出される液を回収する
。The exchange section is provided between the first circulation section and the second circulation section and includes a rough membrane that is impermeable to cells and growth factors but passes nutrients and waste products. The recovery section is provided on the downstream side of the exchange section in the flow path of the second circulation section, and includes a membrane having a roughness that does not allow nutrients to pass through but allows waste products to pass therethrough, and recovers the liquid extracted from the membrane.
また、細胞培養槽の培養条件の制御を容易にするために
、調整槽にもｐＨ１溶存酸素濃度及び温度などの培養条
件の制御手段を設けることが好ましい。Furthermore, in order to facilitate control of the culture conditions in the cell culture tank, it is preferable that the adjustment tank is also provided with means for controlling culture conditions such as pH1 dissolved oxygen concentration and temperature.
（作用）
交換部の膜は細胞や成長因子などの大きさの物質を通さ
ないため、細胞や成長因子は細胞培養槽の系内に留まる
。栄養分と老廃物は交換部の膜を通って細胞培養槽の系
と調整槽の系の間で移動することができる。(Function) Since the membrane of the exchange part does not allow substances of the size of cells and growth factors to pass through, the cells and growth factors remain within the cell culture tank system. Nutrients and waste products can be transferred between the cell culture tank system and the conditioning tank system through the exchange membrane.
回収部の膜は老廃物を通すが栄養分を通さないため、栄
養分は調整槽の系と細胞培養槽の系に留まる。老廃物は
回収部の膜を通って系外に排出される。The membrane in the collection section allows waste to pass through but not nutrients, so nutrients remain in the adjustment tank system and cell culture tank system. Waste products are discharged from the system through a membrane in the collection section.
回収部で排出された液量の新鮮培地が培地流入手段によ
って調整槽に供給される。The amount of fresh culture medium discharged in the recovery section is supplied to the adjustment tank by the medium inflow means.
ｐＨ１溶存酸素濃度、温度などの培養条件は主として培
養槽で制御されるが、好ましい実施例においては調整槽
においても制御される。Culture conditions such as pH1 dissolved oxygen concentration and temperature are mainly controlled in the culture tank, but in preferred embodiments, they are also controlled in the adjustment tank.
（実施例） 図は一実施例を表わす。(Example) The figure represents one embodiment.
２は細胞培養槽であり、培地に細胞が懸濁した培養液４
が収容されている。培養液４の培地は血清、成長因子で
あるＩＬ２、栄養分であるグルコースや蛋白質などを含
んでいる。細胞培養槽２は蓋で閉じられ、培養液４の上
部の空間にはガス制御系（図示路）によって０２、Ｇ
Ｏ２及びＮ２ （又は空気）が供給される。細胞培養
槽２には温度センサ、ｐＨセンサ、溶存酸素濃度センサ
、レベルセンサなどのセンサ類６が設けられている。細
胞培養槽２内には培養液４を攪拌するためにスタータ８
が設けられ、細胞培養槽２の外側の下部にはヒータ（図
示路）が設けられている。ガス制御系。2 is a cell culture tank, and a culture solution 4 in which cells are suspended in a medium
is accommodated. The medium of culture solution 4 contains serum, IL2 which is a growth factor, and glucose and protein which are nutrients. The cell culture tank 2 is closed with a lid, and the space above the culture solution 4 is controlled by a gas control system (path shown).
O2 and N2 (or air) are supplied. The cell culture tank 2 is provided with sensors 6 such as a temperature sensor, a pH sensor, a dissolved oxygen concentration sensor, and a level sensor. A starter 8 is installed in the cell culture tank 2 to stir the culture solution 4.
A heater (path shown) is provided at the lower part of the outside of the cell culture tank 2. Gas control system.
センサ類６及びヒータは培養条件制御手段を構成する。The sensors 6 and the heater constitute culture condition control means.
細胞培養槽２にはまた、培養液４を槽外に循環させるた
めに、チューブの流路１ｏが設けられ、この流路１０の
途中にはポンプ１２が設けられて培養液４を循環させる
。流路１０は交換部１４を経て培養槽２に戻る。The cell culture tank 2 is also provided with a tube flow path 1o in order to circulate the culture solution 4 outside the tank, and a pump 12 is provided in the middle of this flow path 10 to circulate the culture solution 4. The flow path 10 returns to the culture tank 2 via the exchange section 14.
細胞培養槽２にはさらに、新鮮培地供給タンク１６から
ポンプ１８及び電磁弁２０を経て新鮮培地が供給される
供給路２２が設けられている。The cell culture tank 2 is further provided with a supply path 22 through which a fresh medium is supplied from the fresh medium supply tank 16 via the pump 18 and the electromagnetic valve 20.
細胞培養槽２の蓋には細胞を植え込んだり採取したりす
るポート（図示略）が設けられている。The lid of the cell culture tank 2 is provided with a port (not shown) for implanting or collecting cells.
２４は調整槽であり、培地２６が収容されている。培地
２６は栄養分であるグルコースや蛋白質などを含んでい
る。調整槽２４も蓋で閉じられ、培地２６の上部の空間
にはガス制御系（図示略）によって０２、ＧＯ２及びＮ
２ （又は空気）が供給される。調整槽２４にも温度
センサ、ｐ Ｈセンサ、溶存酸素濃度センサ、レベルセ
ンサなどのセンサ類２８が設けられており、調整槽２４
内には培地２６を攪拌するためにスタータ３０が設けら
れ、調整槽２４の外側の下部にはヒータ（図示略）が設
けられている。調整槽２４においてもガス制御系、セン
サ類２８及びヒータは培養条件制御手段を構成する。Reference numeral 24 is an adjustment tank in which a culture medium 26 is accommodated. The medium 26 contains nutrients such as glucose and protein. The adjustment tank 24 is also closed with a lid, and the space above the culture medium 26 is filled with 02, GO2, and N by a gas control system (not shown).
2 (or air) is supplied. The adjustment tank 24 is also provided with sensors 28 such as a temperature sensor, a pH sensor, a dissolved oxygen concentration sensor, and a level sensor.
A starter 30 is provided inside to stir the culture medium 26, and a heater (not shown) is provided outside and at the bottom of the adjustment tank 24. Also in the adjustment tank 24, the gas control system, sensors 28, and heater constitute culture condition control means.
調整槽２４にはまた、培地２６を槽外に循環させるため
に、チューブの流路３２が設けられ、この流路３２の途
中にはポンプ３４が設けられて培地２６を循環させる。The adjustment tank 24 is also provided with a tube flow path 32 in order to circulate the culture medium 26 outside the tank, and a pump 34 is provided in the middle of this flow path 32 to circulate the culture medium 26.
流路３２は交換部１４及び抽出部３６を経て調整槽２４
に戻る。The flow path 32 passes through the exchange section 14 and the extraction section 36 to the adjustment tank 24.
Return to
調整槽２４にも新鮮培地供給タンク１６がらポンプ１８
及び電磁弁２ｏを経て新鮮培地が供給される供給路が設
けられている。The adjustment tank 24 also has a fresh medium supply tank 16 and a pump 18.
A supply path is provided through which fresh culture medium is supplied via the electromagnetic valve 2o.
交換部１４は流路１０と流路３２の間に設けられている
。交換部１４には分画分子量が約］、 ＯＯｏｏの分離
膜３８が設けられており、この分離膜３８を経て細胞培
養槽側の流路１０と調整槽側の流路３２の間で栄養分や
老廃物の交換が行なわれる。分離膜３８としては例えば
限外濾過膜が使用される。The exchange section 14 is provided between the flow path 10 and the flow path 32. The exchange part 14 is provided with a separation membrane 38 with a molecular weight cut-off of approximately Waste products are exchanged. As the separation membrane 38, for example, an ultrafiltration membrane is used.
交換部１４は金属製コネクタ４０によって両流路１０．
３２に交換可能に取りつけられている。The exchange part 14 connects both channels 10. with a metal connector 40.
32 is replaceably attached.
４２は交換部１４を取り替える際に流路１ｏ、３２を閉
じるピンチコックである。42 is a pinch cock that closes the flow paths 1o and 32 when replacing the replacement part 14.
抽出部３６には分画分子量が約４０００以下の分離膜４
４が設けられており、この分離膜４４を経て流路３２か
ら老廃物が培地とともに抽出され、ポンプ４６によって
廃液回収タンク４８に回収される。The extraction section 36 includes a separation membrane 4 having a molecular weight cut-off of about 4000 or less.
4 is provided, and waste products are extracted together with the culture medium from the flow path 32 through this separation membrane 44, and collected into a waste liquid collection tank 48 by a pump 46.
抽出部３６も金属製コネクタ５０によって流路３２に交
換可能に取りつけられている。５２は流路３２を閉じる
ピッチコックである。The extractor 36 is also replaceably attached to the channel 32 by a metal connector 50. 52 is a pitch cock that closes the flow path 32.
次に、本実施例の動作について説明する。Next, the operation of this embodiment will be explained.
細胞培養槽２に培養液４を入れ、調整槽２４に培地２６
を入れ、スタータ８，３０を作動させ、ポンプ１２．３
４を作動させて培養液４、培地２６を循環させながら、
両槽２，２４のｐＨ，溶存酸素濃度及び温度を所定の値
になるように制御する。細胞培養槽２及び調整槽２４に
おける培養条件の制御は、センサ類６，２８の指示によ
ってそれぞれの槽２，２４のガス制御系やヒータを制御
することによって行なわれる。Put the culture solution 4 into the cell culture tank 2, and put the culture medium 26 into the adjustment tank 24.
, start the starters 8 and 30, and turn on the pump 12.3.
4 while circulating the culture solution 4 and medium 26,
The pH, dissolved oxygen concentration, and temperature of both tanks 2 and 24 are controlled to predetermined values. The culture conditions in the cell culture tank 2 and the adjustment tank 24 are controlled by controlling the gas control systems and heaters of the respective tanks 2 and 24 according to instructions from the sensors 6 and 28.
細胞培養槽２の培養液４と調整槽２４の培地２６は矢印
のように交換部１４を通過する。このとき、分離膜３８
では栄養分や老廃物が通過することができるので、流路
３２側から流路１０側に栄養分が補給され、逆に流路１
０側から流路３２側に老廃物が除去される。培養液４中
の細胞や血清の主要成分、成長因子など分子量が約］、
００００より大きいものは分離膜３８を通過せず、細
胞培養槽２に戻される。The culture solution 4 in the cell culture tank 2 and the medium 26 in the adjustment tank 24 pass through the exchange section 14 as indicated by the arrow. At this time, the separation membrane 38
Since nutrients and waste products can pass through, nutrients are replenished from the flow path 32 side to the flow path 10 side, and vice versa.
Waste products are removed from the 0 side to the channel 32 side. The molecular weight of cells in culture medium 4, main components of serum, growth factors, etc. is approximately],
Those larger than 0000 do not pass through the separation membrane 38 and are returned to the cell culture tank 2.
調整槽側の流路３２では、交換部１４がらの培地は抽出
部３６に至る。注出部３６では分画分子量が約４０００
以下の分離膜４４によって老廃物が選択的に分離される
。栄養分は分離膜４４を通過せず、再び調整槽２４に戻
される。老廃物を含んだ培地はポンプ４６によって廃液
回収タンク４８に回収される。In the flow path 32 on the adjustment tank side, the culture medium from the exchange section 14 reaches the extraction section 36 . In the pouring section 36, the molecular weight cut-off is approximately 4000.
Waste products are selectively separated by the following separation membrane 44. The nutrients do not pass through the separation membrane 44 and are returned to the adjustment tank 24 again. The medium containing waste products is collected by a pump 46 into a waste liquid collection tank 48 .
廃液回収タンク４８に培地が回収されることにより、調
整槽２４の培地２６が減少する。センサ類２８にはレベ
ルセンサが備えられているので、レベルセンサの指示に
よって電磁弁２０が調整槽２４方向に切り換えられ、ポ
ンプ１８が作動して、新鮮培地供給タンクＩ６から調整
槽２４に新鮮培地が補給されて調整槽２４の培地２６の
液量が一定に保たれる。By collecting the culture medium into the waste liquid recovery tank 48, the amount of the culture medium 26 in the adjustment tank 24 decreases. Since the sensors 28 are equipped with a level sensor, the solenoid valve 20 is switched in the direction of the adjustment tank 24 according to an instruction from the level sensor, and the pump 18 is activated to supply fresh culture medium from the fresh medium supply tank I6 to the adjustment tank 24. is replenished, and the liquid volume of the culture medium 26 in the adjustment tank 24 is kept constant.
交換部１４で両流路１０，３２の圧力を等しくしておけ
ば細胞培養槽２の培養液４の液量は一定である。しかし
ながら、培養液４が減少した場合に培養液を一定レベル
に戻すために、センサ類６中のレベルセンサの指示によ
って電磁弁２０が細胞培養槽２側に切り換えられ、ポン
プ１８によって新鮮培地供給タンク１６から新鮮培地が
細胞培養槽２に補給される。If the pressures in both channels 10 and 32 are made equal in the exchange section 14, the volume of the culture solution 4 in the cell culture tank 2 is constant. However, in order to return the culture solution to a constant level when the culture solution 4 decreases, the solenoid valve 20 is switched to the cell culture tank 2 side according to an instruction from the level sensor in the sensors 6, and the pump 18 is operated to transfer the culture solution to the fresh culture medium supply tank. Fresh medium is supplied to the cell culture tank 2 from 16.
細胞培養槽２での培養条件の設定と調整槽２４での培養
条件の設定を等しくしておくと、細胞培養槽２における
培養条件の変動を小さく抑えることができる。By setting the culture conditions in the cell culture tank 2 and the culture conditions in the adjustment tank 24 to be the same, fluctuations in the culture conditions in the cell culture tank 2 can be suppressed to a small level.
分離膜３８．４４は目詰りを起こすことがある。Separation membranes 38, 44 may become clogged.
そのような場合、ピンチコック４２．５２を閉じてコネ
クタ４０．５０を外すことにより、分離膜３８．４．４
を無菌的に交換することができる。In such cases, the separation membrane 38.4.4 can be removed by closing the pinch cock 42.52 and disconnecting the connector 40.50.
can be replaced aseptically.
実施例では分離膜３８，４．４が平面状のものであるが
、ホローファイバー形態のものにすることもできる。Although the separation membranes 38, 4.4 are planar in the embodiment, they can also be in the form of hollow fibers.
また、実施例では細胞培養槽２に新鮮培地を供給する供
給路２２を設けているが、この供給路２２を設けず、細
胞培養槽２の培養液４の液量が変動したとき、センサ類
６中のレベルセンサの指示によってポンプ１２．３４の
回転速度を調節することにより、分離膜３８を介して培
養液の液量を調節するようにしてもよい。In addition, in the embodiment, the cell culture tank 2 is provided with the supply path 22 for supplying fresh culture medium, but if this supply path 22 is not provided and the amount of the culture solution 4 in the cell culture tank 2 fluctuates, the sensor The volume of the culture solution may be adjusted through the separation membrane 38 by adjusting the rotational speed of the pump 12.34 according to the instructions from the level sensor 6.
（発明の効果）
本発明では調整槽を設けて細胞培養槽の循環部と調整槽
の循環部の間で栄養分と老廃物の交換を行ない、血清や
成長因子を細胞培養槽の系から出さないようにしたので
、高価な血清や成長因子の利用効率が高まり、維持費が
軽減される。(Effect of the invention) In the present invention, a regulating tank is provided to exchange nutrients and wastes between the circulation section of the cell culture tank and the circulation section of the regulating tank, and serum and growth factors are not released from the cell culture tank system. This increases the efficiency of using expensive serum and growth factors and reduces maintenance costs.
調整槽の循環部には回収部を設けて老廃物を選択的に除
去するようにしたので、老廃物の除去効率が高まる。Since a recovery section is provided in the circulation section of the adjustment tank to selectively remove waste products, the waste removal efficiency is increased.
調整槽においても培養条件を制御するようにすれば、細
胞培養槽だけで培養条件を制御する場合に比べて制御性
能が高まる。If the culture conditions are controlled also in the adjustment tank, the control performance will be improved compared to the case where the culture conditions are controlled only in the cell culture tank.
図は一実施例を示す構成図である。
２・・・・・細胞培養槽、４・・・・・・培養液、６，
２８・・・・・・センサ類、１０．３２・・・・・・流
路、１２，３４゜１８．４．６・・・・・・ポンプ、１
４・・・・・・交換部、１６・・・・・・新鮮培地供給
タンク、２４・・・・・・調整槽、２６・・・・・・培
地、３６・・・・・・抽出部、３８．４４・・・・・・
分離膜、４８・・・・・・廃液回収タンク。
】２−
特許出願人 株式会社島津製作所The figure is a configuration diagram showing one embodiment. 2...Cell culture tank, 4...Culture solution, 6,
28...Sensors, 10.32...Flow path, 12,34°18.4.6...Pump, 1
4... Exchange section, 16... Fresh medium supply tank, 24... Adjustment tank, 26... Culture medium, 36... Extraction section , 38.44...
Separation membrane, 48... Waste liquid collection tank. ]2- Patent applicant: Shimadzu Corporation
Claims (2)
取したりするポート及び培養液を槽外に循環させる第１
の循環部を備えた細胞培養槽と、培養液を槽外に循環さ
せる第２の循環部及び液量に応じて新鮮培地を流入させ
る手段を備えた調整槽と、第１の循環部と第２の循環部
の間に設けられて細胞及び成長因子を通さず栄養分及び
老廃物を通す粗さの膜を備えた交換部と、第２の循環部
の流路で前記交換部より下流側に設けられて栄養分を通
さず老廃物を通す粗さの膜を備えその膜から抽出される
液を回収する回収部とを備えた細胞培養装置。(1) A means for controlling culture conditions, a port for implanting and collecting cells, and a first port for circulating the culture solution outside the tank.
a cell culture tank equipped with a circulation section, a second circulation section for circulating the culture solution outside the tank, and an adjustment tank equipped with a means for introducing fresh culture medium in accordance with the liquid volume; an exchange section provided between the second circulation section and provided with a membrane having a roughness that allows passage of nutrients and waste products but not cells and growth factors; and a flow path of the second circulation section downstream of the exchange section. A cell culture device comprising: a membrane provided with a roughness that allows nutrients to pass through but waste products to pass therethrough; and a recovery section for recovering a liquid extracted from the membrane.
求項１記載の細胞培養装置。(2) The cell culture device according to claim 1, wherein the adjustment tank also includes means for controlling culture conditions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29263388A JP2625990B2 (en) | 1988-11-19 | 1988-11-19 | Cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29263388A JP2625990B2 (en) | 1988-11-19 | 1988-11-19 | Cell culture device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02138962A true JPH02138962A (en) | 1990-05-28 |
| JP2625990B2 JP2625990B2 (en) | 1997-07-02 |
Family
ID=17784317
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP29263388A Expired - Fee Related JP2625990B2 (en) | 1988-11-19 | 1988-11-19 | Cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2625990B2 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05123156A (en) * | 1991-10-31 | 1993-05-21 | Tabai Espec Corp | Culturing device |
| DE19945162A1 (en) * | 1999-09-21 | 2001-04-26 | Herbert Maerkl | Process for culturing cells, membrane module, use of a membrane module and reaction system for culturing cells |
| JP2012165764A (en) * | 2006-07-14 | 2012-09-06 | Dsm Ip Assets Bv | Improved process for culturing cell |
| CN109943528A (en) * | 2019-04-19 | 2019-06-28 | 华子昂 | The method that T cell is produced in enormous quantities |
| CN111484937A (en) * | 2020-05-26 | 2020-08-04 | 上海艾众生物科技有限公司 | Culture medium external circulation updating device for bioreactor |
| JP2022084885A (en) * | 2013-06-24 | 2022-06-07 | ウィルソン ウォルフ マニュファクチャリング コーポレイション | Closed system device and method for gas permeable cell culture work |
-
1988
- 1988-11-19 JP JP29263388A patent/JP2625990B2/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05123156A (en) * | 1991-10-31 | 1993-05-21 | Tabai Espec Corp | Culturing device |
| DE19945162A1 (en) * | 1999-09-21 | 2001-04-26 | Herbert Maerkl | Process for culturing cells, membrane module, use of a membrane module and reaction system for culturing cells |
| JP2012165764A (en) * | 2006-07-14 | 2012-09-06 | Dsm Ip Assets Bv | Improved process for culturing cell |
| JP2014217395A (en) * | 2006-07-14 | 2014-11-20 | ディーエスエム アイピー アセッツ ビー．ブイ． | Improved process for culturing cells |
| US9469865B2 (en) | 2006-07-14 | 2016-10-18 | Dpx Holdings B.V. | Process for the culturing of cells |
| US9670520B2 (en) | 2006-07-14 | 2017-06-06 | Patheon Holdings | B.V. | Process for the culturing of cells |
| JP2022084885A (en) * | 2013-06-24 | 2022-06-07 | ウィルソン ウォルフ マニュファクチャリング コーポレイション | Closed system device and method for gas permeable cell culture work |
| CN109943528A (en) * | 2019-04-19 | 2019-06-28 | 华子昂 | The method that T cell is produced in enormous quantities |
| CN111484937A (en) * | 2020-05-26 | 2020-08-04 | 上海艾众生物科技有限公司 | Culture medium external circulation updating device for bioreactor |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2625990B2 (en) | 1997-07-02 |
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