JPH02126151A - Method and device for applying serum in electrophoretic device - Google Patents

Abstract

PURPOSE:To allow the arbitrary varying and adjusting of a serum applying position so as to meet a film-like support by positioning the support to a receiving base for applying, then positioning an applying device and applying the serum on the support by the vertical movement of the applying device. CONSTITUTION:The support T on a transporting base 100 is stopped when its position is detected by a position detector 49. A film correcting mechanism 30 is lifted by a prescribed distance by a feed screw shaft 22 by which the support T is transferred onto a film correcting plate 320. The correcting plate 320 moves near to or apart from the support T to correct the bend and positional deviation of the support T. The correcting plate 320 is then lifted and the receiving base 521 for applying is moved to the serum applying position and is then lowered to lay the support T on the base 521. The applying device 532 samples the specimen serum with a chip 532A and is then positioned above the serum applying position of the support T. The serum is applied on the support T by vertically moving the chip 532A. The serum applying position is varied and adjusted as desired to meet the support in this way and the positional deviation and bending are thereby automatically corrected.

Description

DETAILED DESCRIPTION OF THE INVENTION Industrial Applicability The present invention relates to a method and apparatus for applying serum in an electric body motion device equipped with a means for correcting curvature of a membranous support. The present invention relates to a coating method and device that can arbitrarily variably adjust the coating position with respect to the body.

BACKGROUND OF THE INVENTION Electrophoresis is widely used in clinical laboratories in medical institutions to test and quantify serum proteins. As is well known, in this electrophoresis method, the serum to be tested is applied onto a membrane-like support such as a cellulose acetate membrane, an electric current is applied to form an image of serum proteins, and the sample is then stained. In this method, after staining in a solution, the portion outside of the serum protein is decolored and quantified using a densitometer. It would be extremely inefficient to perform these electrophoretic analysis steps manually;
This requires great skill, and results may vary depending on the person performing the test. For this reason, various automated devices have conventionally been provided that are capable of automatically and continuously performing various steps of electrophoretic analysis.

What is particularly important in such automated equipment is: 1) that the membrane support is fed smoothly through all processes within the apparatus, and 2) that the membrane support is bent or misaligned throughout the process. Kotona (to be transported.

Problems to be Solved by the Invention However, conventional automated equipment does not have any means for correcting the bending or misalignment of the support, which is bent or misaligned during the previous wetting process. Because the sample had to be transported to the serum application stage, electrophoresis device, and concentration measurement process in its original state, the resulting migration pattern was misaligned or curved relative to the support. If agricultural product measurement is performed in this state, the photometric path of the measurement system will scan obliquely with respect to the electrophoresis pattern. This adversely affects the measurement results, making the obtained data inaccurate and unreliable. Further, if the support is transported while being bent or misaligned, troubles such as jams will occur during the transport process, making it impossible to smoothly transfer the support between each process.

In particular, in the serum application process, if the serum is applied at a position where the support is bent or misaligned, this may cause problems as the fractionation pattern may be deviated from the normal position or formed at an angle. It is.

Furthermore, cellulose acetate membranes are currently widely used as membrane supports for testing serum protein images, but the standards and specifications of cellulose acetate membranes differ from manufacturer to manufacturer. The reality is that the position where the serum should be applied differs from company to company. Therefore, it is necessary to change the serum application position for each support that is manufactured by a different manufacturer and has different standards and specifications. Conventionally, this change in the application position has been performed in the serum application unit including the application table of the electrophoresis device. That is, conventionally, a plurality of coating tables were prepared in advance for each support with different specifications, and a coating table suitable for each support was used.
You can replace it every time the support is different, or use a coating stand with multiple coating positions that suits several supports, or use α
I was doing things like that. However, all of the conventional countermeasures require unnecessary costs, and are extremely complicated to handle, such as requiring the application table to be replaced each time.

This invention was proposed in view of the above points, and it enables the serum application position to be arbitrarily variably adjusted according to the support, and also ensures that the support is not bent or misaligned at the serum application position. The purpose is to make it possible to correct the

Means for Solving the Problems In order to achieve the object described in the list, the present invention employs a serum application method having the following configuration.

In the present invention, the support carried into the serum application position is moved upward to the first position, and the applicator advances the stand below to position the support, and then lowers the support to the second position to apply the serum. The device is characterized by a method in which the device is supported on a table, and an applicator that samples sample serum is moved and positioned in the −L direction of the support, and the serum is applied onto the support by vertical movement of the applicator. When applying this serum, the applicator is placed between the base and the applicator [
[The moving position relative to the support is variably controlled depending on the support at which the Tl liquid is applied.

According to one method of the present study, the support carried into the serum application position is corrected for the bending that occurred in the previous stage. after that,
Move the support -L to the first position and then lower it to the second position to apply the serum.

Furthermore, the present invention provides the following device to achieve the "bipedal purpose"? ? The configuration was adopted.

That is, the present invention provides a support member that is arranged to face both sides of a conveyance table that enters and stops at a serum application device, and that delivers and supports the support of the conveyance table, and a - Membrane correction means 1 equipped with a moving means consisting of a drive source and a transmission mechanism for approaching or moving in the direction of correcting the bending of the support of L, and the membrane trapping IF means, which is raised or lowered by one step, Support 81) Up and down moving means for sequentially driving or moving the material downward from the supporting and bending correction position of the support to a first position above the support and a second position below which the serum is applied; When the applicator moves upward to the first position, the applicator enters and is positioned above the support member and receives the support member that is lowered from the first position to the second position. From the first position to the second
When the applicator is lowered to the position F, it is positioned by moving upward, and by the downward movement of the applicator, the applicator unit 7 applies the serum to the support on the stage 2, and the application unit 7. The film correction means and the upper F movement are linked so that the raising and lowering of the means, the approaching and separating movements of the support member, the forward and backward movements of the base and coating unit, and the vertical movement of the applicator are linked. The gist of the present invention is a serum application device that includes a control unit that controls the operation of an applicator (stand and application unit).

According to one configuration of the present invention, the vertical movement means includes a drive source;
It is constituted by a feed screw shaft which is screwed by the driving of this drive source and screws the film correction means downwardly through the movable body.

Furthermore, according to one configuration of the present invention, the serum sampling position is set at a predetermined position in the forward direction of the applicator (near),
The applicator of the applicator unit that has advanced to this sampling position samples the serum that has moved up and down.

The serum application device according to the present study is equipped with a conveyance table feeding means. This conveyance table feeding means includes a drive source, a gear that meshes with a rack mesh formed on the conveyance table, a transmission means for transmitting the driving force of the drive source to the gear, and a transmission means that transmits the driving force to the gear when the conveyance table is loaded or unloaded. It is composed of a clutch that is connected and released, and a clutch that is switched.

When the conveyance table on which the active support is placed is carried to the serum application position and stopped, the support member of the membrane correction means is moved upward to the support support position and the membrane correction position by the driving of the vertical movement means. Next, by the operation of the membrane correction means, the support member moves toward and away from the support in the calibration capture direction. This proximate separation operation corrects the bending and positional deviation of the support.

Next, the support member supports the support body and moves 1-to the first position. A coating tray enters and is positioned below this support. The support member is driven by the -h upward moving means,
It is lowered to the second position, and the support is placed on the coating pedestal. At the same time, the application unit moves forward to the serum sampling position, samples the serum by the -L downward movement of the applicator, then moves and positions the application unit above the support, and the serum is sampled by the downward movement of the inner cloth device. applied onto a support. Next, the coating unit (applying unit) returns to the −L movement, and then retreats to its original position, and then the support member moves upward together with the support body to the first position again. Next, the coating tray is retracted and returned from below the support. Thereafter, the support member descends and returns to the initial position, and the support body is transferred onto the conveyance table. This completes the serum application. In this case, the moving position of the coating tray and the coating unit to the serum coating position is adjusted in accordance with the support used.

Embodiments Hereinafter, embodiments of the present invention will be described with reference to the drawings.

FIG. 1 shows the overall configuration of the blood jt4 application device in the electrophoresis device according to the present invention, FIG. 2 is a partially cutaway front view of the device at the serum application position, and FIG. A line sectional view, and FIG. 4 is a plan view of FIG. 2 viewed from -.

1) The 11th coating device according to the present invention is placed at the processing position of the serum coating step following the wetting step of stage i7.

In FIGS. 1 to 4, some guide posts 11.11 are erected on both sides of the center of the installation base 10. As shown in FIGS. That L
A support base 12 is supported at the end. Support base 1
A support frame 13 is guided and supported below 2 so as to be movable upward. The bottom plate 1301, that is, the movable base 130
Two central locations of -F are slidably fitted and guided via sleeve-shaped bearings 131 and 131. A feed screw shaft 22 of a vertical movement mechanism 20, which will be described later, is threaded into the center of the base between the bearing parts 131 and 131 of the movable base 130.

-4 part of the plate 14 is placed above the support base 12
40--.

Move the membrane correction mechanism 30 up and down on the support base 12.
Nido mobile machine IJI20 is assembled.

The upper end of the L lower frame motor side 20 is pivotally supported by a vertical feed motor 21 placed near the center on the base 12 and a mounting frame 120 provided on the base stone surface, and the lower end is attached to the center of the bottom plate of the support frame 13. A small diameter pulley 2 is attached to a feed screw shaft 22 which penetrates through the section by screw fitting and is supported by a bearing on the installation base 10, and a shaft 211 that protrudes below the base of the feed motor 21.
3, a large-diameter pulley 24 integrally attached to the -1 end of the feed screw shaft 22, and a belt 25 passed around the two pulleys 23 and 24. Feed screw shaft 2
2 is arranged vertically below 11 between the two guides 11.11, and is screwed into the center of the bottom plate of the support frame 13. The support frame 13 is supported so as to be movable upwardly or upwardly by rotationally driving the feed screw shaft 22 in the forward and reverse directions. Further, it is guided and supported by two guide posts 11.11 so as to be slidable in the vertical direction.

Furthermore, a conveyance table transport mechanism 40 is attached to the support base 12-F to move the conveyance table 100 from the previous process to the next process and vice versa. The conveyor table feeding mechanism 40 is configured as follows.

The drive motor 4 is mounted on one side from the center of the support base 12.
1 is disposed in close proximity to the vertical feed motor 21.

An electromagnetic clutch 42 is disposed on one side of the drive motor 41. A belt 44 is passed between a pulley 43A provided on a shaft protruding below the base of the motor l11 and a pulley 43B provided on an input shaft protruding below the base of the electromagnetic clutch 42. A bearing stand 45 is arranged on the opposite side of the electromagnetic clutch 42 with respect to the center of the support base 12-1-.

Pulleys 46A and 4 (3B) are attached to the output shaft of the electromagnetic clutch 42 and a shaft 451 supported on the bearing stand 45, respectively. A belt 47 is passed around the pulleys 48A and 46B. Gear 48 coaxially with
A and 48B are installed. The gears 48A and 48B are configured as a pair, and partially project in one direction through an opening formed in the plate 14, and are adapted to mesh with rack teeth 110 formed on one side of the conveyance table 100, respectively.

The driving force of the motor 41 is the belt pulley 43A143B.
, 44 to the electromagnetic clutch 42, and is further transmitted to the bearing stand 45 via belt pulleys 46A, 46B, 47.
This causes the gears 48A and 48B to rotate in the same direction in the opposite direction. As a result, the membranous support T
The transport platform 100 on which is placed the rack 110 and the gear 48A,
48B, it is driven back and forth from the previous wetting process to the blood 18 application position shown in FIGS. 1 and 3 and to the next electrophoresis process. Note that in FIG. 1, reference numeral 49 is a position detector that detects the moving position of the conveyance table 100.

A membrane correction mechanism 3 for correcting the bending of the support T on the support frame 13
0 is installed. The film correction mechanism 30 is configured as follows.

The drive motor 300 is arranged on one side from the center of the bottom plate 1301. Left and right sides tf132.1 of the support frame 13
A rotating shaft 301 is pivotally supported between 32. motor 300
The axis of the rotary shaft 301 is orthogonal to the rotating shaft 301, and bevel gears 302 and 303 provided on the respective shafts mesh with each other. A rotation angle detector 30 for detecting the rotational phase of the cam is mounted on the rotation shaft 301.
4 is assembled. Furthermore, the left and right side plates 132.1
A camshaft 305.305 is coaxially supported on 32. The camshaft 305.305 is located on a longitudinal centerline passing through the center of the feed screw shaft 22. Gears 306.30Ei are attached to both left and right sides of the rotating shaft 301. Gear 306.306 is camshaft 3
They mesh with gears 307 and 307 attached to 05 and 305, respectively.

Both ends of the rotating shaft 301 protrude outward from the left and right side plates 132, 132, and cams 308, 30 are attached to the protruding ends.
8 are installed respectively.

Note that the membrane correction mechanisms 3 on both sides of the side plates 132, 132
0 has the same and symmetrical configuration, so only one side thereof will be mainly explained below.

A support 310 is supported on the side plate 132 by a bearing block 309 so as to be movable downward. IJ of shaft 310 is in contact with force 1, 308 via cam follower roller 311. A support block 312 is attached to the upper end of the shaft 310 that protrudes from the bearing block 309.

A pair of guide shafts 313, 313 are horizontally installed on both sides of the two side plates 132 of the support block 312. Slide blocks 314 are respectively mounted on the guide shafts 313 and 313 so as to be slidable. The slide blocks 31C314 are urged toward each other by a spring 315 attached to the guide shaft 313.

A guide groove 312A is formed in the support block 312 in the lateral direction, and one ends of a pair of oval-shaped link members 316 and 316 are fitted and connected to the guide groove 312A via rollers 317 so as to be movable relative to each other. ing. Link member 31
The middle part of 8.316 is pin-coupled to the fil plate 132.The other end is bifurcated into two prongs 5, which are engaged with pins 314A protruding from the slide blocks 314 and 314, respectively.

The link member 318.31B moves the slide block 314.314 through the engagement between the two-pronged portion 316A at the other end and the pin 314A when the support block 312 moves kl'' through the shaft 310 due to the rotation of the cam 308. Slide them so that they are close to each other and φ apart.

The slide block 314.314 has a slide shaft 318.
are erected vertically upward. The left and right slide shafts 318 . Its upper end portions are fitted and connected to fitting grooves 321 and 321 formed at both ends of the membrane correction plate 320. The membrane correction plate 320 also serves as a support member for the support T.1. The side edge of the support T is supported on a stepped portion 322 formed one step higher on the upper surface thereof. Left and right membrane correction plates 3
20.320 is the slide slide shaft 3 when the slide block 314.314 slides in the proximal/pupillary direction.
Proximity to the support T of the conveyor table 100 via 18 -
wi is released, and the bending and positional deviation of the support T are corrected by the contact action.

A serum application mechanism section 5 is installed above one side of the moving rod path of the transport platform 100.
0 is placed. Its configuration is as described below.

A machine frame 510 is arranged on the installation base 500. A coating receiving table 521 is supported at the lower part of the machine frame 510 via an elongated guide member 520 so as to be movable back and forth in the direction of the transport platform 100, that is, in the direction of the serum application position. A drive motor 522 is disposed at the inner bottom of the machine frame 510 on the lower side thereof with its shaft facing upward. A gear 523 provided on the shaft or a rack gear 5 formed on the side of the guide member 520
It meshes with 20A. The driving force of the motor 522 is transmitted to the guide member 520 via rack gears 520A and 523, and the guide member 520 is reciprocated linearly.

A long guide member 530 is supported on the upper part of the machine frame 510 in parallel with the guide member 520 of the coating receiving table 521 so as to be capable of reciprocating linear movement. A holder 531 is supported at the end of the guide member 530 on the side of the transport table. A serum applicator 532 is attached to the holder 531 so as to be movable up and down. Retainer 5
31 has a built-in one-liter lower mechanism 533 that includes a driving source. The applicator 532 is assembled to the holder 531 via this vertical mechanism 533. A drive motor 534 is disposed inside the machine frame 510 below the side of the guide member 530. A gear 535 provided on the shaft meshes with a rack mesh 530A formed on the side of the guide member 530. The driving force of the motor 534 is the rack gear 530A1535.
This is transmitted to the guide member 530 via the guide member 530, and the applicator 532 moves back and forth together with the holder 531 due to its reciprocating linear motion.

That is, it moves back and forth between the serum sampling position, the serum application position above the transport table, and the cleaning position above the installation base 500.

The machine frame 510 is equipped with position detectors 1541 and 542 that detect the movement positions of the guide members 520 and 530, that is, the coating tray 521 and the applicator 532, respectively.

Further, an installation base 50 on the conveyor side with respect to the machine frame 510
A cleaning tank 551 and a wiper roller 552 are arranged parallel to each other and parallel to the longitudinal direction of the coating receiving table 521 and the coating device 532.

After applying the serum, the applicator 532 returns to the top of the cleaning tank 551, and then descends downward to remove the tip 532 at the tip.
A is immersed in a washing solution, and the attached sample serum is washed away. Next, the applicator 532 moves to the position of the wiper roller 552, and the cleaning liquid adhering to the tip 532A is wiped off by the wrapped slip paper.

On the other hand, a serum sampling position is set on the other side of the serum application position on the transport path of the transport table 100. An installation base 560 is placed at this sampling position, and a serum vessel 561 containing sample serum is placed thereon.

The applicator 532 first applies the serum to the serum device 561.
The sample serum in the serum vessel 561 is sampled using the tip 532A.

The film correction mechanism 30 described above moves vertically with a constant stroke by driving the vertical movement mechanism 20. At this time, the mock correction plates 320, 320 are transported from the fourth position at the lowest end. It moves up and down sequentially to the first position A1 at the top end of the table, the second position B1 below it, and the third position C below it, and finally after applying the blood, it moves up and down to the initial position. Return down to the 4th place (ftI). The first position A is a position where the coating receiving table 521 enters below the support T. Further, the second position B is a position for applying serum to the support T, and after the applicator 532 has advanced to the serum sampling position, it is moved and positioned above the transport table. moreover,
In the third position C, the film correction plate 320°320D and the transport platform 1
This is the delivery/transfer position of the support T to and from 00, as well as the bend correction position of the support T.

The above-mentioned vertical movement mechanism 20, film correction mechanism 30, transport table feeding mechanism 40. And the serum application mechanism? ! S50 is driven and controlled by a control unit 200 including a microprocessor built into the electrophoresis device.

The signals from the control unit 200 are sent to the drive motors of each mechanism section, and the drive control controls the operation of each section in conjunction with each other, and controls the timing of each operation. Control of movement position of F, transport platform 1
00 loading, unloading, and stopping position control, as well as advancing and retracting positions of the serum application mechanism section 50 and its application receiving table 521
, the amount of movement of the applicator 532 in the advancing direction, etc. are controlled.

Next, the serum application ability of the present invention with the following device configuration:
■Explain the processing procedure.

Support T that has been bufferized in the previous wetting process
is placed on the conveyor table 100 and carried to the serum application position. Then, gears 48A and 48B of the transport table feeding mechanism 40
When engaged with the rack clutch 110 of the conveyance table 100, the conveyance table 100 is moved by the driving force of the drive motor 41 to the electromagnetic clutch 42,
Rack gear 110, 48A via bearing stand 45, 4.
The engagement of 8B moves the upper plate 7 forward. When moving forward to a predetermined position, the position detector 49
Based on this position detection signal, a signal is sent from the control unit 200 to the electromagnetic crack 42, and the transport platform 100 stops at the serum application position due to the breakage of the electromagnetic crack 42 (Figs. 1 and 2). ). Then, in response to this stop signal, the feed motor 21 of the vertical movement mechanism 20 is controlled to drive in the forward direction, and the screw rotation of the feed screw shaft 22 causes the membrane correction mechanism 30 to move -1 through the movable base 130 of the support frame 13. (predetermined on one side) is held -L. As a result, the membrane correction plate 320.320 moves upward from the lowermost fourth position I to the third position C, and the support T of the transport table 100'2 moves upward from the lowermost fourth position I to the third position C. It will be transferred to. Feed mode 921 has stopped! After 1 unit, the drive motor 3 of the membrane correction mechanism 30
00 is driven and controlled in forward and reverse directions.

And the rotating shaft 301 and the camshaft 305.305
When the cams 308, 308 rotate by a predetermined angle, the slide blocks 314, 314 relatively slide toward and away from each other via the cam mechanism and the link mechanism. Due to this sliding operation, the decompensation plates 320, 320 move toward and away from the support T via the slide shafts 318, 318, and due to the contact and positioning action with the side edges, the support T bends. The positional deviation is corrected (FIG. 5(a)).

Next, the feed motor 21 of the lower crosspiece machine lateral 20 is driven and controlled, and the rotation of the feed screw shaft 22 causes the dummy correction 'a lateral 30 to move upward by a predetermined amount. Then, the membrane correction plates 320, 320 with the support T placed thereon rise from the third position C to the first position A, and are held at the first position A by stopping the drive of the feed motor 21. Thereafter, the pedestal drive motor 522 of the serum application position field 50 is rotationally driven, and the application pedestal 521 moves forward together with the guide member 520 in the direction of the serum application position through the engagement of the rack O gears 520A and 523. Its moving position is detected by a position detector 541.

In response to the input of this position detection signal, the counter 543 counts a predetermined number of pulses, and the counting signal is transmitted to the control unit 7) 20.
Add 5 to 0. Control unit 200 receives this count signal and gives a stop signal to drive motor 522 to stop it. At this stop position, the application receiving table 521 enters below the support T as shown in FIG. 5(b), and is positioned and held at a position corresponding to the serum application position.

Thereafter, the feed screw shaft 22 is reversely rotated by the reverse drive of the feed motor 21 of the vertical movement mechanism 20, and the film correction mechanism 30 is lowered by a predetermined amount. Then, the support T descends from the first position A to the second position B together with the film correction plates 320 and 320, and is placed on the coating receiving table 521 as shown in FIG. 5(C). At approximately the same time that the feed motor 21 stops, the coating unit drive motor 534 is rotationally driven in response to a signal from the control unit 200. Applicator 532 of coating unit 7
moves forward together with the retainer 531 and the guide member 530 through the meshing of the rack gears 530A and 535.

Its moving position is detected by position detector 542. When the position detection signal from the position detector 542 is input, the counter 544 counts a predetermined number of pulses, and the count signal is given to the control unit 200. This count signal represents the amount of movement and position of the applicator 532 to the serum sampling position, and a stop signal is given from the control unit 200 to the drive motor 534 in response to the input of this count signal. When the motor 534 stops, the applicator 532 is positioned and stopped above the serum dish 561 at the serum sampling position. Next, the chip 5 is lowered by the drive of the vertical mechanism 533 built into the holder 531.
After sampling the sample serum in the serum dish 561 at 32A, it returns to the upward direction.

Thereafter, by driving the drive motor 534 in the reverse direction, the applicator 532 is moved backward by an amount corresponding to the number of pulses counted by the counter 544, and when the motor 534 is stopped, the applicator 532 is moved backward as shown in FIG.
As shown in (d), it is positioned and stopped above the serum application position of the support T, and then moved up and down with respect to the support T. By this vertical movement, the serum adhering to the chip 532A is applied to the serum application position on the support T.

After the serum application is completed, the applicator 532 that has returned to the support is moved to the cleaning tank 551 and the IJ roller 552.
It is returned to the top of the chip, repeats the up and down movement, and the chip 5
Serum attached to 32A is washed and removed. Then, it returns to the initial position.

When the applicator 532 returns to its original position, the vertical movement mechanism 2
0, the pseudo correction mechanism 30 moves upward by a predetermined amount, and the support T is lifted from the second position B to the first position A again by the L raising of the membrane correction plates 320 and 320. Next, by driving the drive motor 522, the coating tray 521 moves back from below the support to the initial position and stops. At the same time, due to the operation of the vertical movement mechanism 20, the membrane correction plate 320,
320 descends and returns from the first position A to the fourth position at the lowest end. During this descent, the support T is delivered and transferred onto the conveyor table 100.

Next, the transport table 100 is moved by the operation of the transport table feeding mechanism 40.
moves forward from the serum application position, and the support T that has been coated with serum is carried into the next electrophoresis process.

Effects of the Invention As described above, according to the present invention, the support carried into the serum application position is automatically corrected for misalignment and bending, and is held in exactly aligned position at the serum application position. Ru.

Furthermore, according to the present invention, even when the position of the support to be applied with serum is of different types due to differences in standards and specifications, the application position of the serum application unit can be adjusted according to the position of the support to be used. Since it can be arbitrarily varied and adjusted according to the situation, there is no need for complicated things such as changing and replacing the applicator, and it is also inexpensive in terms of cost.

[Brief explanation of drawings]

FIG. 1 is a partially cutaway side view showing the overall configuration of the serum application device in the electrophoresis apparatus according to the present invention, FIG. 2 is a partially cutaway side view of the device at the serum application position, and FIG. FIG. 4 is a plan view of FIG. 3 seen from above, and FIGS. 5(a) to 5(d) are explanatory diagrams showing fist movements in the serum application procedure. 100・Life・Transportation platform, T11φ・Support body, 30・・φ membrane correction mechanism (means), 320.320・・Model correction plate 300・・Drive motor, 308.308・・Cam, 310・・- Axis, 312...Support block, 313.313--Fist guide shaft, (support member) 314.314--Slide block, 316.31
6. Link member, 318. 318... Slide shaft, 20... Vertical movement mechanism (means), 521... Coating tray, 522... Drive motor, 200... Control unit, 561. ... Serum dish, A... 1st position, B - cloudiest 2nd position, C urn - 3rd position, Dllll - 4th position. Figure 5 (a) 31.8 (b) ()\) oO

Claims (7)

[Claims] (1) In an electrophoresis apparatus equipped with a transport table on which a membrane support is placed and carried in and out from the wetting process to the serum application position and to the next electrophoresis process, the membrane support is carried into the serum application position. The support is moved up to the first position, and the coating pedestal is moved under it to position it, and then the support is lowered to the second position and supported on the coating pedestal, and the sample is A method for applying serum in an electrophoresis apparatus, characterized in that an applicator that has sampled serum is moved and positioned above the support, and the serum is applied onto the support by vertical movement of the applicator. (2) Serum application in the electrophoresis apparatus according to claim (1), characterized in that the moving positions of the application pedestal and the applicator with respect to the support are variably controlled in accordance with the serum application position on the support. Method. (3) Claim (1) characterized in that after correcting the bending of the support carried into the serum application position, the support is moved upward to the first position and then lowered to the second position to apply the serum. )
Or the serum application method in the electrophoresis apparatus described in (2). (4) In an electrophoresis apparatus equipped with a transport platform on which a membranous support is placed and carried in and out from the wetting step to the serum application position and to the next electrophoresis step, entering the serum application position; support members arranged to face both sides of the stopped conveyance table and to deliver and support the supports on the conveyance table;
a membrane correction means comprising a moving means comprising a drive source and a transmission mechanism for moving the support member toward or away from the support member on the carrier in a direction for correcting the bending of the support body; raising or lowering the membrane correction means; a vertical moving means for sequentially moving the support member upward or downward from a support support and bending correction position to a first position above the support member and a second position below the serum application position; an application receiving table that is entered and positioned below the support member when the support member moves upward to the first position and receives the support member that has been lowered from the first position to the second position; When descending from the first position to the second position, the application unit is advanced and positioned above the application unit and applies the serum to the support on the application receiving table by vertical movement of the applicator, and the membrane correction means is raised. - The film correction means, the vertical movement means, and the coating cradle are arranged so that the lowering, the approaching/separating movement of the support member, the forward/backward movement of the coating cradle and the coating unit, and the vertical movement of the applicator are linked. and a control unit that controls the operation of the application unit and the application unit. (5) The above-mentioned vertical movement means comprises a drive source and a feed screw shaft which is rotationally driven by the drive of the drive source and screws the film correction means up and down via a movable body. (4) Serum application device in the electrophoresis device described. (6) A serum sampling position is set at a predetermined position in the forward direction of the application unit, and the applicator of the application unit that has advanced to this sampling position moves up and down to collect blood. (5) Serum application device in the electrophoresis device described. (7) A drive source, a gear that meshes with a rack mesh formed on the conveyance table, a transmission means for transmitting the driving force of the drive source to the gear, and a transmission means that transmits the driving force to the gear when the conveyance table is loaded, unloaded, or stopped. The serum application device in an electrophoresis apparatus according to claim 4, further comprising a conveyance table feeding means comprising: - a releasing clutch;