JPH02117382A - Light applying device for culturing tissue - Google Patents

Abstract

PURPOSE: To enable incubating vessels more than that in the conventional method to be uniformly and efficiently irradiated by light by inserting the tissue incubating vessel into the interior of a coil of a thready illuminant of an optical fiber wire wound like a coil constituted so that the light may be irradiated through a light guide extended from the thread illuminant.

CONSTITUTION: This light irradiation device for tissue incubation has a thready illuminant 2 composed of an optical fiber wire wound like a coil to form a space for storing a vessel 5 for incubating tissue, and at least one terminal of a light guide 4 extended from the thready illuminant as a light incidence terminal. The formation of the space for the incubating vessel 5 (e.g. a test tube) is carried out by using a transparent pipe 1 of an acrylic resin, etc., and winding the optical fiber wire around the periphery thereof.

COPYRIGHT: (C)1990,JPO

Description

[Detailed description of the invention] [Industrial application field] The present invention relates to a light irradiation device used for plant tissue culture.

[Conventional technology]

Plant tissue culture requires irradiation with light, including sunlight and artificial light, for the photosynthetic reaction of the plant, and especially when culturing tissue in test tubes, it is necessary to uniformly apply light to each tissue. Projection becomes an important issue.

Conventionally, a common method for culturing plant tissues is to arrange test tubes containing plant tissues in rows and columns on a shelf and irradiate them with light from fluorescent tubes placed above.

[Problem to be solved by the invention]

Generally, when a fluorescent tube is used as a light source, a maximum illuminance of about 2,000 lux can be obtained, but due to air circulation,
Fluorescent tubes must be placed above the test tubes at a distance of about 503 mm, and in order to compensate for the decrease in illuminance, the number of fluorescent tubes used must be increased and more than 500 V equivalent tubes must be arranged per area. Must be.

Test tubes usually have a diameter of 20+n+1. 5 items with a length of 120m
They are arranged on a shelf, leaning against a 0-tube test tube stand (15al x 30csg), 1.8m long and 1.8m wide.
A space of 603 meters is placed as a passage between the rooms with a height of 2.5 m,
When four shelves are arranged in two rows and test tubes are arranged on each shelf, a maximum of 12,000 test tubes can be accommodated. In order to irradiate all the test tubes placed in each row and each column with the light necessary for plant tissue culture, a fluorescent tube equivalent to 2.7 KV is required, including the ineffective portion. . The ineffective portion, that is, 2.7 Kl (7) approximately 1/2, or 1.35 +
1 becomes thermal energy and raises the ambient temperature. Therefore, an empty mR must be provided to cool the room.Furthermore, when fluorescent tubes are irradiated from above, the amount of light that reaches the test tubes in each stage is at most a few percent (55%) of the total amount of light from the fluorescent tubes. It must be said that even if we focus only on the visible light necessary for one tissue culture, the efficiency will be extremely low.

An object of the present invention is to provide a light irradiation device for tissue culture that eliminates the above-mentioned problems.

[Means to solve the problem]

In order to achieve the above object, the tissue culture light irradiation device according to the present invention has a linear light emitting body formed of an optical fiber wire wound into a coil with a storage space for a tissue culture container formed inside. At least one end surface of the light guide extending from the linear light emitter is a light incident end.

[Principle/Operation] The linear light emitter used in the present invention can be easily obtained by removing the cladding of the optical fiber and making fine scratches on the surface of the exposed core (Japanese Patent Application No. 61-22347).
(Refer to No. 4), a more practical method is to heat or mechanically press a certain area of the optical fiber to deform the interface between the cladding and the core, thereby changing the length of the core covered by the cladding. A linear light-emitting body having a light emitting surface on the directional surface is obtained (patent application filed by the same applicant, filed on 12th lθ, 1986).

This is because the reflection conditions for the skew rays and/or meridional rays that travel spirally within the optical fiber strand are broken and the light is emitted from the curved surface of the optical fiber strand. A linear illuminant connected to a light guide is wound in a coil over the necessary length of the optical fiber strand, and a space necessary for inserting a test tube is formed between each winding. A transparent pipe made of acrylic resin or other material is used to create the insertion space for the test tube.
It is convenient to wrap it around it. Of course, it is not necessary to use a transparent pipe as long as it is possible to give self-shape retention to the winding coil of the linear luminous body.The linear luminous body is basically arranged around a transparent pipe 1, as shown in Figure 2. The light emitting zone 3 can be formed in a required range by wrapping one linear light emitter 2 closely adjacent to each other in a coil shape, or alternatively, as shown in FIG. It is also possible to alternately wrap the curves of a book or three or more transparent pipes 1, 1 to form two or more pairs. In addition, it is not necessarily limited to one linear light emitter, but two or more linear light emitters can be wound in two or more layers above and below a single transparent pipe to form a light emitting zone in the winding of each light emitter. . If sunlight or artificial light is incident on one end or both ends of the light guide 4 that follows the coiled linear illuminant as the light input end, the coiled linear luminescent material will be emitted as shown in Figure 1. The luminescent zone 3 of the body 2 emits light, and the area around the plant P in the test tube 5 inserted into the transparent pipe 1 is irradiated with light.

A light emitting end 6 of a normal optical fiber may be placed above the test tube 5 as an auxiliary light.

〔Example〕

Examples of the present invention are shown below.

Inner diameter 26-2 and outer diameter 30-2 used as a transparent pipe.

A linear illuminant with a diameter of 2 mm was wound in a coil around the outer circumference of an acrylic tube with a length of 100 mm over a range of 421 points from the top, and a culture test tube was inserted into the acrylic tube to emit light through a light guide. Irradiated.

When wrapping the linear light emitters, use two linear light emitters, and wrap each linear light emitter across two acrylic tubes.
The acrylic tube is wound alternately in a coil shape as shown in the figure to form two upper and lower luminous zones. They were wrapped around the tube in the same manner, and the ends of a total of four light guides following both ends of each linear light emitter were used as light incident ends.

Use a floodlight (Hayashi Watch halogen lamp model LA-150, 1501i1) on this linear light emitter, approximately 12/L.
/ -1 lumen of light (therefore, 3 lumens at each input end) was incident, and the light receiving part of the illuminance meter (Kyoritsu Denki Keiki ■ photovoltaic illuminometer model 5200) was applied to the upper opening of the acrylic tube, and the illuminance was measured. When measured, a value of about 500 lux was obtained.

Note that heat rays in the light beam incident on the light guide were removed using a cold mirror (or heat ray absorption filter).

The ultraviolet rays are absorbed by the ultraviolet absorber contained in the optical fiber and removed.

The above-mentioned acrylic tubes wrapped with linear light emitters were bundled into bundles of 50 each and arranged on a shelf. This is about three times the number of test tubes that can be accommodated in the same space as in a conventional test tube room.

〔Effect of the invention〕

As described above, according to the present invention, the light source is brought close to the tube wall of the culture test tube, and the light emitting zone is formed in an annular or cylindrical shape with an arbitrary width, so that it can be uniformly distributed over the required range around the entire circumference of the test tube. Moreover, the light beam can be irradiated efficiently. In addition, according to the present invention, since a secondary light source using optical fibers is used, heat rays and ultraviolet rays contained in the light beam can be removed, making it unnecessary to use air conditioning equipment, and furthermore, Since there is no need to secure space between the test tubes or between the upper and lower transoms for light irradiation, this method has the effect of dramatically increasing space utilization efficiency compared to conventional methods.

[Brief explanation of drawings]

Fig. 1 is a sectional view showing an example of the use of the present invention, Fig. 2 is a perspective view showing an example of how to wind a linear luminous body using a transparent pipe, and Fig. 3 is a winding of a linear luminous body. FIG. 7 is a perspective view showing another example of the line outline. 1... Transparent pipe 2... Linear light emitter 3.
...Light emitting zone 4...Light guide 5...
・Test tube

Claims (1)

[Claims] (1) At least one end surface of a light guide that has a linear light emitting body formed of an optical fiber wire wound into a coil with a storage space for a tissue culture container formed therein, and that is extended to the linear light emitting body. A light irradiation device for tissue culture, characterized in that a light incident end is .