JPH02107963A - Reading apparatus of dna pattern - Google Patents

Abstract

PURPOSE:To reduce the labor required for editing the results of reading and to improve processing capacity by regenerating the digitized image data of a DNA pattern on a display. CONSTITUTION:An image of a DNA film 1 is read for each one line in the direction of the X axis by a one-dimensional image sensor 2. The data of this image are converted into a digital value for each pixel by an A/D conversion circuit 3 and inputted to an image data storage element 4. The whole film is read successively in this way. In an image recognizing-editing processing element 5, the position of existence of a band and the inclination thereof, as well as a DNA sequence, are determined on the basis of said image data, and the informations are outputted, together with the image data themselves, to a file. In the processing element 5, in addition, the image data are transformed into a tile pattern for one pixel and the DNA film image is edited. The edited image is converted into analog signals by a D/A conversion circuit 6 and displayed on an analog RGB display 7. At the same time with this display, a layout of a part being displayed currently is displayed.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a DNA pattern reading device, and in particular,
The present invention relates to an NA pattern reading device that is suitable for storing and reading out read images in a device that reads images resulting from electrophoresis and automatically determines the base sequence of a gene.

[Conventional technology]

In equipment that recognizes gene base sequences from images obtained by labeling genes with radioactive isotopes, fluorescent substances, etc. and performing electrophoresis, the read base sequences are printed on a printer or display as A (amine), C (cytosine), G(
(guanine) and T (thymine).

Therefore, in order to recognize and correct the reading results, as shown in FIG.
20a above.

The DNA sequence 22 was edited by tracing the band 20e, which is the image of the base according to the radioactive position in the lanes 20b, 20c, and 20d, in the direction opposite to the direction of electrophoresis.

With reference to FIG. 26, an editing method using a conventional DNA pattern reading device will be described when reading a film that is the result of electrophoresis labeled with a radioactive isotope or fluorescent material. The DNA film 20 is read by a DNA pattern reader 21 and the DNA sequence is recognized. After this, in the case of conventional devices, the recognized DN
The A sequence 22 was simply output as a file and its contents were displayed as text on the printer 23 or display 24.

[Problem that the invention seeks to solve]

However, the problem with the DNA pattern reading device is that during editing, the user must follow the DNA film 20 again with the naked eye and correct any omissions or recognition errors in the DNA sequence 22.

In other words, it required the same effort as manually inputting the DNA sequence 22.

The present invention has been made to solve the above problems.

An object of the present invention is to provide a technique that can reduce the labor required for editing reading results and improve the overall processing capacity of a DNA pattern reading device.

Another object of the present invention is to provide a technique that can improve the editing accuracy of reading results.

The above and other objects and novel features of the present invention will become apparent from the description of this specification and the accompanying drawings.

[Means for solving problems]

In order to achieve the above object, the present invention labels genes with radioactive isotopes or fluorescent substances, performs electrophoresis, records the results as images on film, reads the images recorded on the film, and detects genes. A DNA pattern reading device that automatically determines the base sequence of a DNA pattern reading device, the device includes a storage device that reads and stores an image on the film, and after reading the entire image on the film, reads an image of necessary image data or The main features include a display means for displaying a concentration graph, and a layout display means for simultaneously displaying the layout of the currently displayed location.

Further, a DNA pattern reading device that labels a gene with a radioactive isotope or a fluorescent substance, performs electrophoresis, reads the distribution of the fluorescent substance from the resulting gel, and automatically determines the base sequence of the gene, a storage means for reading and storing an image on the film; a display means for displaying an image or a density graph of necessary image data after reading the entire image on the film; and a location currently being displayed at the same time as the display means. The present invention is characterized by comprising a layout display means for displaying a layout.

The present invention is also characterized by comprising means for correcting the DNA sequence while viewing the density graph of the image pig displayed by the display means.

Further, the present invention is characterized in that it includes means for extracting data of a predetermined section from the read image data and performing predetermined processing on the data of the extracted section.

Further, the present invention is characterized by comprising means for superimposing a line display indicating a recognized band on the image or density graph of the image data displayed by the display means.

Further, the present invention is characterized in that it includes means for displaying a line indicating a recognized band on the density graph of the image data displayed by the display means and at the same time displaying text of DNA sequence data.

In addition, the DN at the band position where recognition was missing or the position where there was erroneous recognition on the density graph of the image data.
The present invention is characterized by having means for adding and deleting A sequences.

Further, the present invention is characterized in that it includes a display surface scroll processing means that enables display of all DNA patterns.

[Effect]

According to the above-mentioned means, in order to display a DNA film image on a display, it is sufficient to replace each pixel and the density of each pixel with the gradation level of the display. However, the video output of general personal computers is either digital red (R), green (G), and blue (B), or analog RGB with about two gradations, and the gradation display capacity per pixel on the display is limited. low. Therefore, we will consider displaying DNA film images using tile patterns. For example, if one pixel on a DNA film image is formed using a 2 x 2 tile pattern, and the inside of the tile pattern is painted in two colors, if DNA sequence recognition is performed using a 4-gradation scanner, the DNA film image will be As long as there is pixel-by-pixel density data, it may be possible to reproduce the DNA film image on the display during recognition. However, since the scanner used in the DNA pattern reading device has a gradation resolution capability of about 256 gradations, image deterioration is unavoidable when displaying a film image even if a tile pattern is used. If we consider simultaneously displaying concentration graphs in a cross section along the center line of each lane on the DNA film, a display connected to a normal personal computer has a resolution of about 400 vertical dots. Therefore, even if the density graphs of the four lanes on the DNA film are arranged so that they do not overlap spatially, if one dot is assigned to one gradation, about 100 gradations can be displayed.

Normally, there is a gradation difference of at least 3/256 to 4/256 between the location of a band and its background on a DNA film, so if a density graph is displayed, the location of the band can be identified with sufficient accuracy. become. In this way, the read DNA film image and density graph are simultaneously displayed on the display using a tile pattern of size 1 x 2 or larger, a line display indicating the recognized band is superimposed on this, and this image is further displayed. It has a function to add and delete DNA sequences at band positions that are not recognized or erroneously recognized on the display/density graph, and it has a scroll function so that all DNA film images can be displayed in an overlapping manner. By doing so, the user can check the results, edit, and make corrections by looking only at the display surface, and the effort required for this can be significantly reduced. Furthermore, the accuracy of editing the reading results can be improved.

In addition, by displaying the layout of the currently displayed area at the same time as displaying the image or density graph of the necessary image data, it becomes possible to check the results and make editing corrections more easily, which reduces the effort required. can be further reduced significantly. Furthermore, the accuracy of editing the reading results can be further improved.

[Embodiments of the invention]

Hereinafter, one embodiment of the present invention will be specifically described using the drawings.

Note that throughout the description of the embodiments, parts having the same functions are given the same reference numerals, and repeated explanations thereof will be omitted.

[Example ■]

FIG. 1 is a block diagram showing a schematic configuration of a DNA pattern reading device according to Example I of the present invention.

As shown in FIG. 1, the DNA pattern reading device of this embodiment I includes a one-dimensional image sensor 2 for reading an image on a DNA film 1, and an analog/digital (A) for converting the output from analog to digital. /D
) A conversion circuit 3, an image data storage section 4 for storing data for each pixel, and a circuit for determining the base sequence from the image data read from the image data storage section 4, and for displaying and modifying the results. It is comprised of an image recognition and editing processing section 5 made up of a computer (CPS), a digital-to-analog (D/A) conversion circuit 6 for displaying read image data, an analog RGB display 7, and the like.

Next, the operation of the DNA pattern reading apparatus of Example 2 will be briefly explained.

As shown in FIG. 1, the image of the DNA film 1 is scanned with the X-axis direction as the main scanning direction and the Y-axis direction as the sub-scanning direction, and is read by the one-dimensional image sensor 2 every line in the X-axis direction. It will be done.

The image data read by this -dimensional image sensor 2 is converted from an analog value to a digital value in an analog-to-digital conversion circuit 3 for each pixel. here,
The density of one pixel is expressed as an 8-bit digital value. The image data for each line converted into digital values is input to the image data storage section 4. In this way, the entire film is read continuously. The image recognition and editing processing section 5 determines the position of the band, its slope, and the DNA sequence based on this digitized image data, and outputs the information together with the image data itself to a file. In addition, the image recognition editing processing section 5 converts the image data into a tile pattern pixel by pixel, and converts the image data into a tile pattern.
The film image is edited, and the digital signal of this DNA film image is converted into an analog signal by a digital/analog (D/A) conversion circuit 6, which is displayed on an analog RGB display 7. At the same time, a concentration graph at the center line of each lane is also displayed.

At this time, the image recognition and editing processing section 5 simultaneously displays a line in consideration of the smiling face for the recognized band based on the information on the band existing position and its inclination. At this time, the text of the DNA sequence data is also displayed at the same time (FIG. 15). The image recognition and editing processing unit 5 has the function of accepting and executing requests for sequence deletion and addition from the user at any position on the DNA film image and the density graph, allowing the user to simply look at the display. You can edit the NA sequence with accuracy.

Next, the DNA sequence editing method in the image recognition editing processing section 5 will be explained in detail.

FIG. 2 is a block diagram showing the functional configuration for explaining the DNA sequence editing method of the DNA pattern reading device of the present embodiment (1) shown in FIG. It is a device.

The central processing unit 300 has an initial processing function 301, a DNA sequence editing execution processing function 302, a concentration graph display processing function 303, and a smiling cursor display processing function 304.
, band deletion processing function 305, band addition processing function 30
6. Cursor movement processing function 307, display screen scrolling processing function 308, analog RGB image display processing function 30
9 and an editing end processing function 310.

When confirming the DNA stamp reading result by the central processing unit 300, a keyboard 311 for inputting commands etc. is provided as an input device, and an analog RGB display 7 connected to an analog RGB output board 312 for displaying a DNA film image is provided as an output device. It is being The output of the editing completion processing function 310 is recorded on a floppy disk (FD) 313, a hard disk (HD) 314, a digital audio tape (DAT) 315, a memory (MEMORY) 316, and the like.

FIG. 3 is a flowchart showing the processing procedure of the initial processing function 301 in FIG.

The processing procedure of the initial processing function 301 is as shown in FIGS. 2 and 3. First, in step 101, a floppy disk (FD) 313, a hard disk (HD) 31
4. Import film image information and recognition information stored in a recording medium (FIG. 2) such as a digital audio tape (DAT) 315.

Next, in step 102, the upper limit of the film image displayed on the analog RGB display 7 (FIG. 2),
After determining the lower limit, the DN is displayed on the analog RGB display 7 by analog RGB image display processing in step 103.
Display the image of part A.

Next, in step 104, the DNA sequence is displayed on the right side of the analog RGB display 7, and in step 105, a cursor K is displayed on the film partial image.

Next, in step 106, a cursor 2K is displayed on the DNA code corresponding to the cursor K on the film partial image.

Next, in step 107, the recognized band=16- is marked with a line or point, and the process moves on to the next process, that is, DNA sequence editing execution process.

FIG. 4 is a flowchart showing the processing procedure of the DNA sequence editing execution processing function 302 in FIG.

As shown in FIG. 4, the processing procedure of the DNA sequence editing execution processing function 302 is such that, in step 401, it is determined whether the input from the keyboard 311 (FIG. 2) is a request for displaying a graph. If yes (Y
ES), a concentration graph display process is performed in step 408, and if the determination is NO, the process moves to the next step 4.2.

In step 402, it is determined whether the input from the keyboard 311 is a request to display a smiling cursor, and if the determination is YES, a smiling cursor display process is performed in step 409, and if the determination is no. If (No), next step 4
Move to 03.

In step 403, it is determined whether the input from the keyboard 311 is a request for band deletion processing, and if the determination is YES, band deletion processing is performed in step 410, and if the determination is NO ( No
), the process moves to the next step 404.

In step 404, it is determined whether the input from the keyboard 311 is a band addition processing request. If the determination is YES, band addition processing is performed in step 411, and if the determination is NO (YES), band addition processing is performed in step 411. No
), the process moves to the first step 405.

In step 405, it is determined whether the input from the keyboard 311 is a cursor movement process request,
If the determination is YES, the cursor position on the film image display surface or the DNA sequence display surface is moved in step 412, and if the determination is NO, the next The process moves to step 406.

In step 406, it is determined whether the input from the keyboard 311 is a display screen scroll processing request, and if the determination is YES,
In step 413, the display screen is scrolled, and if the determination is NO, the process moves to the next step 407.

In step 407, it is determined whether the input from the keyboard 311 is an editing completion processing request, and if the determination is YES, step 4
At step 14, the recognition information is updated by editing, and editing completion processing is performed.

FIG. 5 shows the concentration graph display processing function 30 in FIG.
3 is a flowchart showing the processing procedure of step 3.

As shown in FIG. 5, the processing procedure of the density graph display processing function 303 is as follows: First, in step 501, the image display of the DNA sequence is erased, and in step 502, image data on the central axis of each lane of the film image is obtained. . next,
In step 503, the maximum value (dmax) and minimum value (dmin) in the image data of the film image currently being displayed are obtained. Next, in step 504, amplitude M = (display range L per rune) x (density of each pixel - dmin)
Display the graph as / (dmax-dmin+1),
In step 505, a recognition marking is displayed as cursor 2 on the concentration graph display surface, and the concentration graph display process is completed (return).

FIG. 6 is a flowchart showing the processing procedure of the smiling cursor display processing function 304 in FIG.
Figure 7A is a diagram showing the contents of film image information, Figure 7B is a diagram showing the contents of film image information;
The figure is a diagram showing the contents of recognition information.

The processing procedure of the smiling cursor display processing function 304 is as shown in FIG. 6. First, in step 601, a normalized position corresponding to the cursor position is determined based on the content of the recognition information shown in FIG. 7B. Here, the normalized position is shown in Figure 8 (a diagram showing the normalized axis of the central axis of 4 lenses).
This refers to the band position after coordinate transformation from the center axis of each lane to the center axis of four lanes while taking into account the influence of smiling as shown in FIG.

Next, in step 602, smiling information for each lane is obtained using the normalized position obtained in step 6020-1. In step 6Q3, the coordinates of the left end and right end of each lane corresponding to the cursor position are calculated based on the smiling information. In step 604, step 6
Connect and display the coordinates of the left and right ends of each lane obtained in step 03 with a straight line and end the smiling cursor display process (r
FIG. 9 is a flowchart showing the processing procedure of the band deletion processing function 305 in FIG. 2.

As shown in FIG. 9, the processing procedure of the band deletion processing function 305 is as shown in FIG. To disable.

Next, in step 902, the corresponding DNA code is deleted from the DNA sequence display, and in step 903, the recognized marking on the film image is deleted, and the band deletion process is completed (return).

FIG. 10 shows the band addition processing function 306 in FIG.
2 is a flowchart showing a processing procedure.

As shown in FIG. 10, the processing procedure of the band addition processing function 806 is as shown in FIG. Insert additional entries to the information.

Next, in step 1002, the deviation of the band Y coordinate between the normalization axis and the lane center point and the Y coordinate between the right end and left end of the band display are determined according to the internal division ratio of the entry before and after the additional insertion entry of the recognition information. Calculate and set the respective coordinate shifts.

Next, in step 1003, the DNA code of the lane where the cursor is located is set in the entry of recognition information.

Next, in step 1004, a DNA code is inserted at a position corresponding to the DNA sequence display, and step 1
At step 005, a marking of the recognized band is displayed on the mouth surface of the film image, and the band addition process is completed (ret
urn).

FIG. 11 shows the cursor movement processing function 30 in FIG.
7 is a flowchart showing the processing procedure of step 7.

The processing procedure of the cursor movement processing function 307 is as shown in FIG.
It is determined whether the input from 11 is a request to move the right cursor between lanes, and if the determination is YES (YES)
If so, the process moves to step 1109, and if the determination is no (NO), the process moves to the next step 1102.

In step 1109, it is determined whether the current cursor position is on the fourth lane, and if the determination is YES, the process moves to step 1111, and the center of the fourth lane is set to the X coordinate of the cursor position. Set the X coordinate of the axis. Judgment in step 1109 is NO
If so, the process moves to step 1110, and the inter-lane distance is added to the X coordinate of the Carso position.

In the pre-penetration step 1102, it is determined whether the input from the keyboard 311 is a request to move the left cursor between lanes, and if the determination is YES, the process moves to step 1103, and the answer is NO. If so, proceed to the next step 1106.

In step 1103, it is determined whether the current cursor position is on the fourth lane, and if the determination is YES, the process moves to step 1105, and the fourth lane is placed at the X coordinate of the cursor position. Set the X coordinate of the central axis. The determination in step 1103 is No (N
o), the process moves to the next step 11o4, and the inter-lane distance is subtracted from the X coordinate of the cursor position.

In the step 11o6, it is determined whether the input from the keyboard 311 is a request to move the cursor up, and if the determination is YES, the process moves to step 1108, and r is added to the Y coordinate of the cursor position.
Add r 1 u ("+1"'). If the determination is No, the process moves to step 1107, and tL I I+ is subtracted from the Y coordinate of the cursor position ("-1").
Press L to end the cursor movement process (return).

FIG. 12 is a flowchart showing the processing procedure of the display screen scroll processing function 308 in FIG.

The processing procedure of the display image mouth scroll processing function 308 is as follows:
As shown in FIG. 12, in step 12o1, it is determined whether the input from the keyboard 311 is an image page up request, and if the determination is YES (YE
S), move to step 1203, shift the film image display upper and lower limits by +1 aperture, and
), the process moves to step 1202 and the film image display upper limit is determined.

Shift the lower limit by 11 units.

Next, in step 1204, the currently displayed film image and the recognized band markings are erased.

Next, in step 1205, a partial image of the film is displayed as an analog RGB image, and in step 1206, markings of recognized bands are displayed on the film image display.

Next, in step 1207, a cursor K is displayed at the lowest band marking position on the film image display,
In step 12o8, a cursor 2K is displayed on the DNA code of the DNA sequence corresponding to the band pointed to by the cursor, and the display image front scrolling process is completed.

FIG. 13 is a flowchart showing the processing procedure of the analog RGB image display processing function 309 in FIG.

The processing procedure of the analog RGB image display processing function 309 is as follows:
As shown in FIG. 13, in step 13o1, gamma correction (γ=0...
45).

Next, in step 1302, edge enhancement (4 neighborhoods, coefficient 1.0) of the image data to be displayed is performed.

Next, in step 1303, the image data after the image processing and the display coordinates of each data on the display are passed to the analog RGB output board 312, a display request is made by digital-to-analog (D/A) conversion, and the analog R
End GB image display processing ( r e j u r
n) 11 FIG. 14 is a flowchart showing the processing procedure of the editing end processing function 310 in FIG.

As shown in FIG. 14, the processing procedure of the editing completion processing function 310 is as follows: In step 14o1, it is determined whether the key human effort is a result update request (a request to update the contents of the recognition information shown in FIG. 7B due to editing work). If the determination is YES, the process moves to step 1402, and the floppy disk (FD) 313. Hard disk (HD) 315, memory 314. Digital Audio Tape (DAT) 315. Update the recognition information stored in etc. If the determination is NO, the editing work is stopped and the recognition information is not updated.

As can be seen from the above description, it is usually necessary to add or delete markings of recognized bands on the image display of a DNA film at as precise a point as possible.

Otherwise, when bands are added in places where the band spacing is narrow, overlapping bands (with overlapping normalized positions) that should not appear in the first place may appear.

Also, it is best to add a band at the position with the highest density on the image display, but it is difficult to visually determine the position of the density peak if the band exists in a wide range with a certain level of density. Become.

However, according to this embodiment, the image data of the DNA film 1 is reproduced on the analog RGB display 7, as shown in FIG.
By displaying the density of each DNA sequence 41 of the NA film image 4° in the density graph 42, the difference in density is converted into a shift in spatial coordinates, so the peak position (line) P, that is, the correct band position, can be calculated. Decisions become easier. Therefore, accurate results can be checked and corrections can be made quickly. In addition, in Fig. 15, 4°a is D
Each lane on the displayed image of NA film 1.

40b is the center line of each lane 40a, 40c is the band, 4
3 is a line display displayed at the correct band position in the concentration graph 42, and 44 is the text of the DNA sequence.

FIG. 16 is a block diagram showing the functional configuration of a result display method that displays the layout of the film image being displayed in the DNA pattern reading device according to the present embodiment I shown in FIG. 1 at the same time as the DNA pattern is displayed.

300 is a central processing unit of the DNA pattern reading device, which includes a keyboard 311 for inputting commands and the like as an input device when checking the reading results, and an analog RGB output board 3 for displaying a DNA film image as an output device.
An analog RGB display 7 connected to 12 is connected.

The functions of the result display method for displaying the layout of the film image being displayed by the central processing unit 300 at the same time as the DNA pattern display are: the initial processing function 301, the display screen scroll processing function 308, and the analog RGB image display processing function 3.
09, layout display processing function 317 consists of each processing function block.

The display screen scroll processing function 308 and analog R
Each processing procedure of the GB image display processing function 309 is the same as the processing procedure described above using FIGS. 12 and 13, so a description thereof will be omitted here.

FIG. 17 is a flowchart showing the processing procedure of the initial processing function 3.1 in FIG. 16.

As shown in FIG. 17, the processing procedure of the initial processing function 301 is as shown in FIG. Take in information.

Next, in step 17o2, the upper and lower limits of the film image to be displayed on the analog RGB display 7 are determined, and in step 17o3, the partial image of the DNA is displayed on the display through analog RGB image processing.

After this, in step 1704, the DNA sequence is displayed on the right side of the display. Next step 1705
, a layout display is performed to show where in the whole the film image currently being displayed is located.

Furthermore, the already recognized band on the film image display is marked with a line or point, and the process proceeds to display screen scroll processing.

FIG. 18 shows the layout display processing 31 in FIG.
7 is a flowchart showing the processing procedure of step 7.

The processing procedure of the layout display processing 317 is as shown in FIG. 18. First, in step 1801), the layout display area on the analog RGB display 7 is refreshed.

Next, in step 1802, an outer frame representing the entire scan plane is displayed.

Next, in step 1803, the film image information shown in FIG. 7A is referred to, and the image data acquisition start position (sp
), the image data acquisition end position (ep) is obtained, and the film existing range is displayed in a layout. The coordinates (S, e) of the display start and end of the existing range are as follows: 5=dX (ep/m) e, assuming that the entire length of the scanning surface is m, and m in the display display of the analog RGB display 7 is d. =dX(sp/m).

In step 1804, a layout display is made of the part where the film image is being displayed based on the image display lower limit (LP) and image display upper limit (HP). The display coordinates (Ce, Cs) are
Respectively, Ce=dX(HP/m)Cs=dX(LP/m).

With this configuration, the layout of the film image being displayed on the DNA pattern reading device is displayed at the same time as the DNA pattern is displayed, so that accurate results can be confirmed more quickly and editing can be corrected.

Furthermore, the accuracy of editing the reading results can be improved.

[Example ■]

FIG. 19 is a block configuration diagram showing a schematic configuration of a fluorescence detection type electrophoresis device related to the DNA pattern reading device of Example 2 of the present invention.

As shown in FIG. 19, the fluorescence detection type electrophoresis device according to the present embodiment (2) includes a light source 201 for exciting fluorescence, a fiber 202 for guiding light from the light source 201, an electrophoresis section 203 for performing electrophoresis, Upper and lower electrodes 204 for applying voltage to the electrophoresis section 203, a power source 205 for applying voltage to the electrodes 204, and a lens system 20 for receiving fluorescence.
6. An optical amplifier 207 for amplifying an optical signal; a one-dimensional optical sensor 208 for converting the image optically amplified by the optical amplifier 207 into an electrical signal; and a one-dimensional optical sensor 208 for amplifying the electrical signal from the -dimensional optical sensor 208. amplifier 209, said amplifier 2
An analog-to-digital (A/D) conversion circuit 210 for digitizing electrical signals from 09;
A memory 241 that stores data obtained by the digital (A/D) conversion circuit 210, an interface unit 242 that connects the memory 241 and an output device, and an address that specifies an address for writing data to the memory 241. A generation circuit 243, a control unit 244 for controlling the entire system, a display 245 for displaying images,
Film printer 246 for saving as film
Consists of etc.

Next, the operation of the fluorescence detection type electrophoresis apparatus of Example 2 will be explained.

In FIG. 19, first, the electrophoresis section 203
As shown in the figure, a gel 222 made of polyacrylamide or the like is sandwiched between glass 221 on both sides. Light from the light source 201 passes through the optical fiber 202 and is irradiated onto the gel 222 of the electrophoresis section 203 from the lower left side of the electrophoresis section 203 so as to form an optical path 213 .

The phosphor present in the optical path 213 within the gel 222 is excited and generates fluorescence 224.

This fluorescence 224 is transmitted to the lens system 2 as shown in FIG.
06, the light is focused, and reaches the optical amplifier 207. The optical amplifier 207 amplifies the intensity of light by several thousand to tens of thousands of times. This amplified light reaches the -dimensional optical sensor 208 and is converted into an electrical signal. The electrical signal obtained by the -dimensional optical sensor 208 is amplified to an appropriate level by an amplifier 209 and sent to an analog-to-digital conversion circuit 210. Here, the number of quantizations in Example H is assumed to be 8 bits, for example. The timing of digitalization is given by the control section 244. Also,
This timing signal is also applied to the address generation circuit 243 to synchronize the address generated from the address generation circuit 243 with data writing. In this way, the image data is stored in memory 241 in chronological order.

The detailed configuration of the memory 241 and address generation circuit 243 is shown in FIG. 21 (block configuration diagram).

The address generation circuit 243 is composed of an address conversion circuit 431, an X counter 432, and an X counter 433, as shown in FIG.

The X counter 432 is set, for example, from II OII to "23
This is a counter that repeatedly counts "2376" pieces up to 75". Also, a carry (carry signal) is generated when the count value is 2375. This carry is added to the X counter 433, and the X counter 433 is incremented by 1. Ru.

On the other hand, the address conversion circuit 431 converts addresses in order to use the memory 241 efficiently.

Normally, the capacity of the memory 241 is determined by the number of address input lines N
Then, the number is rr 2 n to the Nth power. Therefore, the addresses range from "O" to "2-1"'.

In this embodiment (2), since the number of data in the X direction is 2376, the minimum is 12 bits (211 = 2048
12=4096) addresses are required. Therefore, if you simply add the output of the X counter 433 to the memory address specification as the upper address and the output of the 241 becomes inefficient. In order to avoid this, the address conversion circuit 431 performs conversion as shown in equation (1).

Memory address=YX2376+X (1) To perform the calculation of equation (1), an adder and a multiplier are basically required.

In this embodiment, the multiplication of the first term on the right side of equation (1) is calculated in advance, and the ROM or RAM with an address greater than or equal to the maximum value of Y is
etc. are written. Therefore, multiplication by 1 becomes a simple ROM access, which simplifies the circuit, makes it inexpensive and high-speed, and can accommodate changes in the number of pixels in the X direction. The address generation circuit is comprised of an adder that adds the outputs of this ROM and the X counter.

These operations will be explained using the timing signal D shown in FIG.

First, the case where the output from the analog-to-digital converter 210 is written into the memory 241 will be described (FIG. 19(a)). The X counter 432 is incremented at the falling edge of the clock coming from the control unit 244.

When reaching 2375, a carry is output and the X counter 433 is incremented by 1 as shown in FIG.

Data from analog-to-digital converter 210 (second
The input data (hatched portion in FIG. 2) is output to the data line in synchronization with the falling edge of the clock, and is written into the memory 241 at the rising edge of the stable strobe signal of the address and data.

Next, the case of reading out data from the memory 241 to output data will be explained using FIG. 22(b). Similarly to writing, a clock is applied to read and an address is generated. Also, when the strobe signal becomes true (high level), the output signal is output during that period and the interface section 2
Sent to 42.

The interface section 242 has an analog-to-digital conversion function in addition to the function of directly outputting digital data from the memory. Based on the commands from the control unit 244, this data is directly converted into digital audio data.
It can be output to a tape recorder 247 or to a display 245 as a video signal converted into an analog signal.

Further, if necessary, the image can be output to an optical film printer 246 and saved.

The output results are as shown in Figure 23, Figure 24A, and Figure 24B.
An electrophoretic pattern of NA is obtained as shown in the figure.

In this case, as shown in FIG. 23, the horizontal axis represents the optical path 213 (
20), and the vertical axis is the direction of time. The bands are arranged so that the minimum pitch on the vertical axis is approximately equal throughout due to the fluorescence distribution when passing through the position of the optical path 213 (FIG. 20). In Figure 20, 32 to 35 are D
Each lane of the NA image is shown. Also, the 24th A
In the figure and Fig. 24B, the bending and tilting (called smiling) like bands 52 and 53 are due to various causes such as variations in temperature distribution that occur during electrophoresis and variations in the state of the gel. known to occur.

Even in such a case, according to the present embodiment (2), the position of the band between each lane 32 to 35 can be reliably estimated.

The DNA pattern reading device according to the embodiment (2) of the present invention reads out the data stored in the memory 241 of the fluorescence detection electrophoresis device, as explained using FIG. 22(b), and The data is input to the image recognition/editing processing section 5 of Embodiment (2) shown in FIG. 1, and the same processing as in Embodiment I is performed.

In other words, genes are labeled with a fluorophore, electrophoresed, and
A display means for directly reading the phosphor distribution from the resulting gel and storing it in the memory 241, and displaying an image or a concentration graph of necessary image data of the stored phosphor distribution, from the read image data. means for extracting data of a predetermined section and applying predetermined processing to the data of the extracted section; a line display indicating a recognized band being superimposed on the image or density graph of the image data displayed by the display means; means for displaying the image of the image data displayed by the display means and a line indicating the recognized band on the density graph; means for displaying the text of the DNA sequence data at the same time as the image of the image data displayed by the display means; and a means for adding or deleting a DNA sequence at a band position that is not recognized or a position that is erroneously recognized on the concentration graph, and a means for scrolling the display surface so that all DNA patterns can be displayed. It is prepared.

As can be seen from the above explanation, according to the present Example H, the distribution of the phosphor is directly read from the electrophoresis gel, and the memory 2
41, so as in Example I, D
This has the advantage that it is not necessary to create NA films each time.

The present invention has been specifically explained above based on examples, but
It goes without saying that the present invention is not limited to the embodiments described above, and can be modified in various ways without departing from the spirit thereof.

〔Effect of the invention〕

As described above, according to the present invention, the image data of the digitized DNA pattern is reproduced on the display, and the editing function on the DNA image or on the density graph of each lane is provided to the automatic DNA reader. By importing, you can quickly check accurate results and make edits. In addition, the layout of the film image being displayed on the DNA pattern reader is
Since it is displayed at the same time as the pattern display, you can check accurate results more quickly and make editing corrections.

As a result, there is no need to perform the troublesome work of visually rereading the DNA film when confirming results, which was essential in conventional DNA pattern reading devices, and the labor burden can be significantly reduced.

Furthermore, the accuracy of editing the reading results can be improved.

Furthermore, since there is no need to take out the DNA file that has been read once again from the storage location, it can also be used to prevent deterioration of easily damaged DNA film.

[Brief explanation of drawings]

FIG. 1 is a block diagram showing a schematic configuration of a DNA pattern reading device according to Example 2 of the present invention, and FIG. 2 is a block diagram showing a schematic configuration of a DNA pattern reading device according to Example I shown in FIG.
FIG. 3 is a block diagram showing the functional configuration for explaining the sequence editing method. FIG. 3 is a flowchart showing the processing procedure of the initial processing function in FIG. 2. FIG. FIG. 5 is a flowchart showing the processing procedure of the concentration graph display processing function in FIG. 2; FIG. 6 is a flowchart showing the processing procedure of the smiling cursor display processing function in FIG. 2; The figure shows the contents of film image information, and FIG. 7B shows the contents of recognition information. Fig. 8 is a diagram showing the normalization axis of the center axis of the 4 lanes, Fig. 9 is a flowchart showing the processing procedure of the band deletion processing function in Fig. 2, and Fig. 10 is a diagram showing the band addition processing in Fig. 2. Flowchart showing the processing procedure of the function; FIG. 11 is a flowchart showing the processing procedure of the cursor movement processing function in FIG. 2; FIG. 12 is a flowchart showing the processing procedure of the display screen scrolling processing function in FIG. 2; Figure 13 shows the analog RG in Figure 2.
A flowchart showing the processing procedure of the B image display processing function,
14 is a flowchart showing the processing procedure of the editing end processing function in FIG. 2; FIG. 15 is a display screen read and displayed by the DNA pattern reading device of Example I shown in FIG. 1; FIG. 16 is a block diagram showing the functional configuration of a result display method that displays the layout of the film image being displayed in the DNA pattern reading device according to the present embodiment (2) shown in FIG. 1 at the same time as the DNA pattern is displayed. is a flowchart showing the processing procedure of the initial processing function in FIG. 16, and FIG. 18 is a flowchart showing the processing procedure of the initial processing function in FIG.
7 is a flowchart showing the processing procedure of step 7, FIG. 19 is a block configuration diagram showing the schematic structure of a fluorescence detection type electrophoresis device related to the DNA pattern reading device of Example 3 of the present invention, and FIG. 21 is a block configuration diagram showing the detailed configuration of the memory and address generation circuit shown in FIG. 20, and FIG. 23, 24A, and 24B are diagrams showing DNA electrophoresis patterns. FIGS. 25 and 26 are diagrams showing conventional DNA electrophoresis patterns. It is a figure for explaining the problem of a button reading device. In the figure, 1... DNA film, 2... -dimensional image sensor 23... analog-digital (A/D) conversion circuit, 4... image data storage section, 5... image recognition editing processing section , 6... Digital-to-analog (D/A) conversion circuit, 7... Analog RGB display, 2゜1
... light source, 202 ... optical fiber, 203 ... electrophoresis section, 204 ... electrode, 205 ... power supply, 20
6... Lens system, 2o7... Optical amplifier, 208...
- Dimensional optical sensor, 209...Amplifier, 210...Analog-to-digital (A/D) conversion circuit, 242...Interface section, 243.Address generation circuit, 244
...Control unit, 245...Display, 246...
Film printer, 300...Central processing unit, 301
... Initial processing function, 302 ... DNA sequence editing execution processing function, 303 ... Concentration graph display processing function, 304 ... Smiling cursor display processing function, 3
05...Band deletion processing function, 306...Band addition processing function, 307...Cursor movement processing function, 30
8... Display screen scroll processing function, 309... Analog RGB image display processing function, 310... Edit end processing function, 317... Layout display processing function.

Claims (8)

[Claims] (1) Label genes with radioactive isotopes or fluorescent substances, perform electrophoresis, and record the results as images on film.
A DNA pattern reading device that reads the image recorded on the film and automatically determines the base sequence of the gene, comprising a storage means for reading and storing the image on the film, and a storage means for reading the entire image on the film. 1. A DNA pattern reading device comprising: a display means for displaying an image or a density graph of necessary image data, and a layout display means for simultaneously displaying a layout of a currently displayed location. (2) A DNA pattern reading device that labels a gene with a radioactive isotope or a fluorescent substance, performs electrophoresis, reads the distribution of the fluorescent substance from the resulting gel, and automatically determines the base sequence of the gene, a storage means for reading and storing the image on the film; a display means for displaying an image or a density graph of necessary image data after reading the entire image on the film; and a display means currently displaying the image at the same time as the display means. A DNA pattern reading device comprising a layout display means for displaying a layout of a location. (3) In the DNA pattern reading device according to claim 1 or 2, the DNA sequence is corrected while looking at the image or density graph of the image data displayed by the display means and the layout of the currently displayed location. A DNA pattern reading device characterized by comprising means. (4) DN according to any one of claims 1 to 3 above.
A DNA pattern reading device characterized in that the DNA pattern reading device comprises means for extracting data of a predetermined section from read image data and performing predetermined processing on the data of the extracted section. (5) DN according to any one of claims 1 to 4 above.
A DNA pattern reading device, characterized in that the DNA pattern reading device comprises means for superimposing a line display indicating a recognized band on the density graph of the image data displayed by the display means. (6) DN according to any one of claims 1 to 5 above.
A pattern reading device, characterized in that the device includes means for displaying a line indicating a recognized band on the density graph of the image data displayed by the display means and at the same time displaying text of DNA sequence data. Pattern reader. (7) DN according to any one of claims 1 to 6
A DNA pattern reading device, characterized in that the device is equipped with means for adding or deleting a DNA sequence at a band position where recognition is missing or a position where erroneous recognition occurs on the density graph of the image data. reading device. (8) DN according to any one of claims 1 to 7.
A DNA pattern reading device, characterized in that the device is equipped with display screen scroll processing means that enables display of all DNA patterns.