JPH01247079A - Control of ph of culture medium - Google Patents
Control of ph of culture mediumInfo
- Publication number
- JPH01247079A JPH01247079A JP7498388A JP7498388A JPH01247079A JP H01247079 A JPH01247079 A JP H01247079A JP 7498388 A JP7498388 A JP 7498388A JP 7498388 A JP7498388 A JP 7498388A JP H01247079 A JPH01247079 A JP H01247079A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- culture
- calcium carbonate
- microorganisms
- calcium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000001963 growth medium Substances 0.000 title claims abstract description 8
- 239000002609 medium Substances 0.000 claims abstract description 17
- 244000005700 microbiome Species 0.000 claims abstract description 9
- 150000003839 salts Chemical class 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 16
- 229910000019 calcium carbonate Inorganic materials 0.000 abstract description 7
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 abstract description 6
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 abstract description 5
- 238000006386 neutralization reaction Methods 0.000 abstract description 5
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 3
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 238000011109 contamination Methods 0.000 abstract description 3
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 abstract description 3
- 230000002427 irreversible effect Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 abstract description 2
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract description 2
- 229940088417 precipitated calcium carbonate Drugs 0.000 abstract description 2
- 239000000306 component Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000005273 aeration Methods 0.000 description 3
- 230000003472 neutralizing effect Effects 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 235000011132 calcium sulphate Nutrition 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012533 medium component Substances 0.000 description 2
- 238000010979 pH adjustment Methods 0.000 description 2
- 239000013076 target substance Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は微生物培養に関し、特に培地のｐＨ調整ンこ関
する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to microbial culture, and particularly to pH adjustment of a culture medium.
従来、この稀の培地１）Ｈ調整は、例えば第３図および
第４図に示すような三角フラスコ１に菌体を含む培地２
を入れ、通気用ポンプ４から通気管６を通して通気する
装置を使用し、中和用試薬７をコック８を開いて培地２
に滴下してｐＨメーター９でｐＨ値を測定しながら中和
反応させることにより最適］）Ｈ値に合わせていた。Conventionally, this rare medium 1) H adjustment has been carried out by placing a medium 2 containing bacterial cells in an Erlenmeyer flask 1 as shown in FIGS. 3 and 4, for example.
Using an aeration device from the aeration pump 4 through the aeration pipe 6, the neutralization reagent 7 is poured into the medium 2 by opening the cock 8.
The pH was adjusted to the optimum H value by adding the solution dropwise to the solution and performing a neutralization reaction while measuring the pH value with a pH meter 9.
上述した従来の生育中の培地のｐＨ調整は、第３図滅菌
済みの中和用試薬７を滴下、あるいはメンブレンフィル
ターを通した中和用試薬を滴下する方法をとっているが
、実験室レベルで簡単な培養を行いたい時には手間や費
用がかかりすぎること、ｐＨの急激な一時的変化を防ぐ
ため中和用試薬の濃度をうすくすると、特に培養時間が
長くなると、加える試薬の量が増えてしまい、他の培地
成分の濃度が薄くなり、培養に影響が出てしまい再現性
に問題が出ること、常に安定した一定のｐＨを保つため
に費やす労力や費用が多くかかること、汚染の確率が高
くなること、などの欠点がある。The above-mentioned conventional pH adjustment of the growing medium is carried out by dropping the sterilized neutralizing reagent 7 shown in Figure 3, or by dropping the neutralizing reagent passed through a membrane filter, but this is not possible at a laboratory level. However, if you want to perform a simple culture in a culture medium, it will take too much time and money, and if you dilute the concentration of the neutralizing reagent to prevent sudden temporary changes in pH, the amount of reagent added will increase, especially if the culture time becomes longer. The concentration of other medium components becomes diluted, which affects the culture and causes problems in reproducibility. It takes a lot of effort and money to maintain a stable and constant pH at all times, and the probability of contamination increases. There are disadvantages such as being expensive.
本発明の培地のｐＨ調整法は、微生物培養用の培地内に
、水に対して難溶性の塩を添加している。In the method for adjusting the pH of a culture medium of the present invention, a salt that is sparingly soluble in water is added to a culture medium for microorganisms.
本発明によれば、難溶性の塩である炭酸カルシウムを入
れることによって培地のｐＨを常に７．０に保つことが
可能であり、労力、費用の面で大変経済的であるだけで
なく、汚染の可能性も小さいという特長を有する。According to the present invention, it is possible to constantly maintain the pH of the culture medium at 7.0 by adding calcium carbonate, which is a sparingly soluble salt, which is not only very economical in terms of labor and cost, but also prevents contamination. It has the advantage that the possibility of
次に、本発明について図面を参照して説明する。 Next, the present invention will be explained with reference to the drawings.
第１図は本発明による培養の一実施例を示したもので第
２図はそのＡ−Ａ’での断面図である。FIG. 1 shows an embodiment of the culture according to the present invention, and FIG. 2 is a cross-sectional view taken along line AA'.
培養により培地のＩ）Ｈが下がってしまう系において、
まず培養容器内１に培地組成成分と共に過剰の炭酸カル
シウム３を入れ、滅菌後、微生物を接種して培養を開始
する。微生物の生育によって生じた酸は、沈殿している
炭酸カルシウム２と中和反応を起こし、水素イオン濃度
と水素イオン濃度が等しくなり、培地のｐＨは常に７．
０付近で安定する。In a system where the I)H of the medium decreases due to culture,
First, an excess of calcium carbonate 3 is placed in a culture container 1 together with the medium components, and after sterilization, microorganisms are inoculated and culture is started. The acid generated by the growth of microorganisms causes a neutralization reaction with the precipitated calcium carbonate 2, and the hydrogen ion concentration becomes equal to the hydrogen ion concentration, and the pH of the medium is always 7.
It stabilizes around 0.
ただし本発明を適用するにあたって、微生物によって生
成される目的物質や酸素の生産などが過剰のカルシウム
イオン等によって阻害を受けたり、不可逆的な反応を起
こさないこと、最適ｐＨが７．０に近いことなどを確認
した上で本発明を適用すべきである。However, in applying the present invention, it is necessary to ensure that the production of target substances and oxygen produced by microorganisms is not inhibited by excessive calcium ions, etc., and that irreversible reactions do not occur, and that the optimum pH is close to 7.0. The present invention should be applied after confirming the following.
前述の実施例では難溶性の塩として炭酸カルシウムをあ
げたが硫酸カルシウム類や硫酸バリウムも本発明に適用
できる。カルシウムイオンや炭酸イオンにより、目的物
質や酵素生産が阻害されたり不可逆的な反応を起こした
りする場合には硫酸カルシウムや硫酸バリウムの方が有
効である。In the above embodiments, calcium carbonate was used as the poorly soluble salt, but calcium sulfates and barium sulfate can also be applied to the present invention. Calcium sulfate and barium sulfate are more effective when calcium ions or carbonate ions inhibit the production of target substances or enzymes or cause irreversible reactions.
以上説明したように、本発明は、微生物の培養によって
培地のｐＨが下がってしまう系において、水に対して難
溶性の炭酸カルシウムを入れるという簡単な方法によっ
て培地のｐＨを７．０付近に保つことができる効果があ
る。As explained above, the present invention maintains the pH of the medium at around 7.0 by a simple method of adding calcium carbonate, which is sparingly soluble in water, in a system where the pH of the medium decreases due to the cultivation of microorganisms. There is an effect that can be done.
第１図は本発明の一実施例による培地のｐＨ調整方法を
示す側面図、第２図は第１図のＡ−Ａ’での断面図、第
３図は従来の培地のｐＨ調整方法を示す側面図、第４図
は第２図のＢ−Ｂ’での断面図である。
１・・・・・・三角フラスコ、２・・・・・・菌体を含
む培地、３・・・・・・炭酸カルシウム、４・・・・・
・通気用ポンプ、５・・・・・・栓、６・・・・・・通
気管、７・・・・・・中和用試薬、８・・・・・・コッ
ク、９・・・・・・ｐＨメーター、１゛０・・・・・・
ｐＨセンサー、１１・・・・・・ガラス管。
代理人 弁理士 内 原 晋
ｔｏ：ＰＨｉ：ｒ−’ｌ’ ７ 問
％３図FIG. 1 is a side view showing a method for adjusting the pH of a medium according to an embodiment of the present invention, FIG. 2 is a cross-sectional view taken along line A-A' in FIG. 1, and FIG. The side view shown in FIG. 4 is a sectional view taken along line BB' in FIG. 2. 1...Erlenmeyer flask, 2...Medium containing bacterial cells, 3...Calcium carbonate, 4...
- Ventilation pump, 5... Stopper, 6... Ventilation pipe, 7... Neutralization reagent, 8... Cock, 9... ...pH meter, 1゛0...
pH sensor, 11...Glass tube. Agent Patent Attorney Susumu Uchiharato:PHi:r-'l' 7 Question %3 Figure
Claims (1)
ことにより培地のｐＨを常に一定に保つことが可能なこ
とを特徴とする培地のｐＨ調整方法。1. A method for adjusting the pH of a microorganism culture medium, characterized in that the pH of the medium can be kept constant by adding a salt that is sparingly soluble in water to the medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7498388A JPH01247079A (en) | 1988-03-28 | 1988-03-28 | Control of ph of culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7498388A JPH01247079A (en) | 1988-03-28 | 1988-03-28 | Control of ph of culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01247079A true JPH01247079A (en) | 1989-10-02 |
Family
ID=13563028
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7498388A Pending JPH01247079A (en) | 1988-03-28 | 1988-03-28 | Control of ph of culture medium |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01247079A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005045050A1 (en) * | 2003-11-10 | 2005-05-19 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Process for the cultivation of microorganisms of the genus thraustochytriales |
-
1988
- 1988-03-28 JP JP7498388A patent/JPH01247079A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005045050A1 (en) * | 2003-11-10 | 2005-05-19 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Process for the cultivation of microorganisms of the genus thraustochytriales |
| EA011877B1 (en) * | 2003-11-10 | 2009-06-30 | Нутринова Ньютришн Спешиэлтис Энд Фуд Ингридиентс Гмбх | Process for the cultivation of microorganisms of the genus thraustochytriales |
| AU2004287953B2 (en) * | 2003-11-10 | 2010-04-29 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Process for the cultivation of microorganisms of the genus thraustochytriales |
| US8889382B2 (en) | 2003-11-10 | 2014-11-18 | Markus Luy | Process for cultivating microorganisms of the genus Thraustochytriales |
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