JPH01225461A - Nutrient food containing octacosanal - Google Patents

Abstract

PURPOSE:To obtain the subject food having health-promoting and fatigue relieving effects, by using octacosanal as an active component. CONSTITUTION:The objective food such as jelly, chocolate, sport drink or coffee milk is produced by compounding octacosanal.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention is directed to an industrial application containing octacosanal as an active ingredient.
The present invention relates to a nutritional food that has the effect of increasing physical strength, relieving fatigue, etc.

Prior art and its problems Octacosanal is a linear higher saturated aliphatic aldehyde with 28 carbon atoms, and is known to be contained in the lipids of plant leaves [for example, cabbage, proccoli: Harald, H, oh.

Harald H, O, Schmid et al.; Hoche, Seilers, Zett, Ficiol, Kem (H
oppe-8eyler's Z, Phymol,
Chem, Perennial Rye Grass 462-466 (1969): Perennial Rye Grass and Fathen: Jenni-fer E, Aneb
one) etc.; dam. Fisi, Lipits (Chem, Ph
ys, Lipids) 4, 37-46 (197
0) see]. However, the physiological activity of octacosanal is not yet known, and therefore its industrial use has not been attempted.

Means for Solving the Problems The present inventor conducted various studies on octacosanal for the purpose of industrial utilization, and as a result, confirmed its excellent physiological effects on living organisms. Namely.

When administered to living organisms, octacosanal increased liver glycogen content, and in organisms with high liver glycogen content, promoted liver glycogen consumption during exercise, and as a reflection of this, significantly increased blood lactate levels. From these findings, it is recognized that octacosanal exerts an effect on increasing physical strength and recovering from fatigue in living organisms.

Therefore, the present invention provides a nutritional food that contains octacosanal as an active ingredient and has excellent effects on improving physical strength and recovering from fatigue for humans and animals.

Examples of the octacosanal-containing food products of the present invention include solid or liquid foods in the usual forms, such as jelly, chocolate, sports drinks, coffee milk, etc. containing octacosanal, but also common pharmaceutical products, such as tablets or capsules. It may be a pharmaceutical form used in the field.

The present invention will be specifically explained in the following experimental examples and examples, but the present invention is not limited thereto.

Experimental Example 1 Sprague-Dawley
) strain male rats (5 weeks old, 9 weight, 100-130 y)
(Nippon Talea Co., Ltd.) was used. Five to six rats per cage were placed in a breeding room adjusted to 20 ± 1'(1') under a light-dark cycle with a dark period from 7:00 p.m. to 7:00 p.m. and a light period from 7:00 p.m. to 7:00 p.m. bred.

During the 3-day pre-breeding period, commercially available powdered feed CE-2 (Nippon Talea Co., Ltd.) was fed twice a day, and thereafter the rats were divided into two groups and fed with the basal diet shown in Table 1 below or with octacosanal 0.02. Meal-feeding was carried out twice, from 8:00 to 8:30 a.m. and from 6:00 p.m. to 6:30 p.m. The water.

They were allowed to take it freely at any time.

Table 1 Basic food composition Corn starch 45.5 g casein
24.5 sucrose
11.0 Soybean oil 6.0 Salt mixture umbrella 17.0 Choline chloride 0.3 Vitamin mixture - 20.7 Cellulose 5.0100.0 y From the 8th day of feeding the experimental diet, each of the two groups of rats received 2 The mice were subjected to swimming exercise under flowing water for 2 weeks, once a day, for 30 minutes from 9:00 pm each time, with a weight weighing 1-296 weighing 9 pounds around their necks.

Feed the animals between 8:00 and 8:30 the next day.

Eight hours later, at 16:30, 5 g of sucrose per body weight hy was orally administered in the form of an aqueous solution. 22:30 6 hours after administration
Seven rats in each group were sacrificed by decapitation for 1 minute, and the remaining rats were placed with a weight equivalent to 496 body weight on their necks and placed under running water.
The animals were subjected to swimming exercise for hours, and immediately after completion, they were sacrificed by decapitation.

Blood and part of the liver were immediately collected from the sacrificed rats, the blood was separated into plasma, and the amount of lactate was measured using a lactate measurement kit manufactured by Boehringer.
Lo) etc.; J, App.
L Phyaol, ), 28.234-236 (
The amount of glycogen was measured according to the method of (1970).

The results are shown in Table 1 below.

Table 1 Control group 3.092±0.264 8.860±
0.975 test group 3.198±0.256 1
1.933±1.148 control group 43.360±3.
681 32.388±3.272 test group 48.
298±2.000 28.447±3.595 The results in Table 1 above indicate that octacosanal significantly promotes the consumption of liver glycogen due to exercise and has the effect of significantly increasing the production of blood lactic acid. It shows.

Experimental Example 2 Sprague-Dawl L/-(Sprague-Dawl
ey) strain male rats (4 appropriate ages, average initial weight 92 g) (Nippon Talea; Tokyo) were used. Two rats were placed in each cage and raised in a breeding room adjusted to 21±1° C. under a light/dark cycle with a dark period from 7:00 to 7:00 and a light period from 7:00 to 19:00.

During the 2-day pre-breeding period, the animals were fed twice a day from 8 to 10 a.m. and from 2 p.m.
0-22:00 and then twice a day, 8-9:00 and 19-2
At 0 o'clock, each animal was meal-fed with commercially available powdered feed CE-2 (Nippon Talea Co., Ltd.). Water is always free 1
I made him ingest this.

All rats were given swimming training under running water for 30 minutes from 9:00 pm twice, on day 12 of rearing and at mouth of day 13.

Feed at 8:00 to 9:00 on the 15th day of rearing, and after 8 hours
At 7 o'clock, 18.75 g/g of sucrose was given over 30 minutes. Then, at 18:30, the rats were divided into halves, the test group received 50 μg/160 g body weight of octacosanal in soybean oil suspension (7,81 rnl/kg body weight), and the control group received the same amount of soybean oil. was administered orally. At 22:00, 3.5 hours after administration, half of the rats in each group were sacrificed by decapitation.

The remaining rats were placed with a weight equivalent to 9 weight 296 on their necks and subjected to swimming exercise under running water for 1 hour, and immediately after the exercise was completed, they were sacrificed by decapitation.

The liver was immediately collected from the sacrificed rats, and the amount of glycogen was measured by the Lo method described in Experimental Example 1. The results are shown in Table 2 below.

Table 2 Control group 84.1±1.9 60.9±3.8 Test group 92.9±2.0 54.4±4.6+j The results in Table 2 above are derived from octacosanal or loaded sugar. It has been shown to have the effect of increasing liver glycogen storage and significantly promoting its consumption through exercise.

Therefore, octacosanal is useful as a nutritional ingredient, especially during exercise.

Next, an example will be shown. Octacosanal is extracted from cane stem peel with hexane and concentrated to 6796 (hereinafter referred to as
Octacosanal concentrate) was used.

Example 1 Jelly Confectionery Octacosanal Concentrate 0.25y Corn Oil
12.25.

Pectin 25° Sucrose 200g Citric acid 5g Fruit juice extract 30° Fruit juice flavor 3g Water 250ml Octacosanal concentrate was added to corn oil and dissolved by heating. Separately, an aqueous pectin solution was prepared, and octacosanal-containing corn oil was added thereto to thoroughly emulsify it, and then the remaining ingredients were mixed and concentrated in a conventional manner to obtain a jelly confectionery.

The resulting product contains 0.067% octacosanal.

Example 2 1 each other Octafsanal concentrate 0.04.

Corn oil 2g mixed nib
15゜sucrose
40.

Cocoa butter 20.

Powdered milk 20y Vanilla flavor Appropriate amount Lecithin 0.3g After premixing octacosanal-containing corn oil prepared in the same manner as in Example 1 with lecithin.

The remaining ingredients were added and milk chocolate was prepared according to a conventional method.

The resulting product contains 0.027% octacosanal.

Example 3 Liquid Octacosanal Concentrate 0.04 ct Corn Oil 2g Sucrose
60.

Citric acid 2g Vitamin C1y Salt 0.3.

Magnesium chloride (Mgcl, 6Hρ) 0.0
5g potassium chloride (KCI) 0.
8.

Grapefruit Essence 2g Grapefruit Concentrate 8g Water
100+iL Corn oil containing octacosanal prepared in the same manner as in Example 1 was mixed with grapefruit essence in advance, and then added to an aqueous solution in which the remaining ingredients were dissolved and thoroughly mixed to obtain a liquid preparation. This liquid is.

Especially suitable for drinking during exercise.

The resulting product contains 0.02396 octacosanal.

Example 4 1232 m Octacosanal concentrate 4g Corn oil 200.

Skimmed milk 1101 Coffee infusion 70t Sucrose
12k.

Corn syrup 2kg Coffee color 100mL Coffee essence
150 Warmness Octacosanal-containing corn oil prepared in the same manner as in Example 1 was added in advance to skim milk 201.

After uniformly emulsifying, the remaining skim milk was added and the remaining ingredients were added and dissolved to prepare coffee milk.

The resulting product contains 0.02796 octacosanal.

Experimental Example 5 15 g of soft capsule Octacosanal, 15 g of natural vitamin E (Emix 80; Eisai ■) and 70 g of corn oil were mixed and dissolved by heating. Use this liquid as the content liquid.

Soft capsules containing 300 mg of liquid content per capsule were prepared according to a conventional method using the following formulation for the skin.

Skin formula: 100 parts gelatin glycerin
20〃 D-Sorbitol 10〃

Claims (1)

[Claims] Nutritional food containing octacosanal as an active ingredient