JPH01202281A - Deoxidation agent for culture of anaerobic bacteria - Google Patents
Deoxidation agent for culture of anaerobic bacteriaInfo
- Publication number
- JPH01202281A JPH01202281A JP2697688A JP2697688A JPH01202281A JP H01202281 A JPH01202281 A JP H01202281A JP 2697688 A JP2697688 A JP 2697688A JP 2697688 A JP2697688 A JP 2697688A JP H01202281 A JPH01202281 A JP H01202281A
- Authority
- JP
- Japan
- Prior art keywords
- water
- activated carbon
- calcium silicate
- amount
- oxygen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001148471 unidentified anaerobic bacterium Species 0.000 title abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910001868 water Inorganic materials 0.000 claims abstract description 15
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 12
- 239000000378 calcium silicate Substances 0.000 claims abstract description 9
- 229910052918 calcium silicate Inorganic materials 0.000 claims abstract description 9
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 7
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 6
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 6
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 6
- 150000003839 salts Chemical class 0.000 claims abstract description 6
- 229910001854 alkali hydroxide Inorganic materials 0.000 claims abstract description 5
- -1 alkali hydroxide compound Chemical class 0.000 claims abstract description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 17
- 229910052760 oxygen Inorganic materials 0.000 claims description 17
- 239000001301 oxygen Substances 0.000 claims description 17
- 229940123973 Oxygen scavenger Drugs 0.000 claims description 15
- 239000006096 absorbing agent Substances 0.000 claims description 7
- 239000002075 main ingredient Substances 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000002585 base Substances 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 abstract 3
- 238000013329 compounding Methods 0.000 abstract 1
- 238000004078 waterproofing Methods 0.000 abstract 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 28
- 239000001569 carbon dioxide Substances 0.000 description 14
- 229910002092 carbon dioxide Inorganic materials 0.000 description 14
- 239000007789 gas Substances 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000004745 nonwoven fabric Substances 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 235000010378 sodium ascorbate Nutrition 0.000 description 2
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 2
- 229960005055 sodium ascorbate Drugs 0.000 description 2
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 2
- 229920002803 thermoplastic polyurethane Polymers 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920001328 Polyvinylidene chloride Polymers 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000008044 alkali metal hydroxides Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- OSMSIOKMMFKNIL-UHFFFAOYSA-N calcium;silicon Chemical compound [Ca]=[Si] OSMSIOKMMFKNIL-UHFFFAOYSA-N 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 239000005033 polyvinylidene chloride Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】 〈産業上の利用分野〉 本発明は、嫌気細菌の培養に適した脱酸素剤に関する。[Detailed description of the invention] <Industrial application field> The present invention relates to an oxygen scavenger suitable for culturing anaerobic bacteria.
〈従来の技術〉
脱酸素剤を用いた嫌気性細菌の培養として例えば特公昭
５８−４９１３号公報に示されており、手軽に培養が行
なうことができ、各機関で用いられるようになってきた
。<Prior art> For example, Japanese Patent Publication No. 58-4913 discloses the cultivation of anaerobic bacteria using an oxygen scavenger, which allows for easy cultivation and has come to be used in various institutions. .
この嫌気性細菌の培養に用いる脱酸素剤として、特公昭
６１−４３９９５号公報に示すように、アスコルビン酸
またはその塩、第１鉄塩を主剤とした粉末タイプの脱酸
素剤を用い雰囲気中の酸素濃度および炭酸ガス濃度を調
整するため脱酸素剤の組成比を変えて行うものがあった
。この脱酸素剤は、予じめ水を加えないで、組成中の結
晶水により徐々に酸素吸収、炭酸ガス発生反応が進むた
め、培養雰囲気が嫌気性細菌の培養に適した状態になる
まで約６時間を要してしまい、時間の短縮が望まれてい
た。As shown in Japanese Patent Publication No. 61-43995, a powder-type oxygen absorber containing ascorbic acid, its salt, or ferrous salt as its main ingredient is used as an oxygen absorber to culture this anaerobic bacteria. In some cases, the composition ratio of the oxygen scavenger was changed in order to adjust the oxygen concentration and carbon dioxide concentration. This oxygen scavenger does not add water in advance; the crystallized water in its composition gradually absorbs oxygen and generates carbon dioxide, so it takes approximately It took 6 hours, and it was hoped that the time could be shortened.
この短時間で培養雰囲気を達成する脱酸素剤として特開
昭６１−１３８５３３号公報に示されるようにアスコル
ビン酸ナトリウムおよび第一鉄塩の混合水溶液を含浸し
易すい繊維質基材に含浸したものを提案した。As shown in Japanese Patent Application Laid-Open No. 138533/1983, an oxygen scavenger that can achieve a culture atmosphere in a short time is an easily impregnable fibrous base material impregnated with a mixed aqueous solution of sodium ascorbate and ferrous salt. proposed.
この脱酸素剤は、１時間後に嫌気性細菌培養雰囲気を達
成するが、経時的に炭酸ガスの発生が続き、最終的には
約２０％まで達してしまい、嫌気性細欝の培養雰囲気が
高濃度すぎてしまう問題が発生した。This oxygen scavenger achieves an anaerobic bacterial culture atmosphere after one hour, but carbon dioxide gas continues to be generated over time, eventually reaching about 20%, and the anaerobic culture atmosphere becomes too high. A problem occurred where the concentration was too high.
この問題を解決するため別の繊維質基材に例えば水酸化
カルシウム水溶液を含浸させて用いる手段が考えられる
が、このようにして用いると炭酸ガスの初期における吸
収速度が早く、初期濃度が低く、最終濃度は高く、炭酸
ガスの濃度を一定範囲に保つのは困難であった。In order to solve this problem, it is possible to use another fibrous base material impregnated with, for example, an aqueous calcium hydroxide solution, but when used in this way, the initial absorption rate of carbon dioxide gas is fast and the initial concentration is low. The final concentration was high and it was difficult to maintain the carbon dioxide concentration within a certain range.
また、溶液タイプの脱酸素剤は、一定の雰囲気を達成す
るためにアスコルビン酸ナトリウムと第１鉄塩との濃度
調整が微妙で取扱が煩雑なものであった。In addition, solution-type oxygen scavengers are complicated to handle because the concentration of sodium ascorbate and ferrous salt must be carefully adjusted to achieve a constant atmosphere.
〈解決しようとする課題〉
本発明の目的は、脱酸素剤の取扱いが簡便な粉末タイプ
で２時間以内、好ましくは１時間以内に好ましい嫌気性
細菌の培養雰囲気（酸素濃度０．１Ｘ以下、炭酸ガス濃
度５〜１０％）とすることができ、しかもその雰囲気を
保つことができる脱酸素剤を提供することである。<Problems to be Solved> The object of the present invention is to use a powder type oxygen scavenger that is easy to handle, and to provide a suitable culture atmosphere for anaerobic bacteria (oxygen concentration 0.1X or less, carbon dioxide An object of the present invention is to provide an oxygen scavenger that can maintain a gas concentration of 5 to 10%) and maintain that atmosphere.
〈課題を解決する為の手段〉
本発明は、アスコルビン酸またはその塩を主剤とし、水
酸化アルカリ化合物、第１銖塩、活性炭、ケイ酸カルシ
ウムおよび水を加えてなる脱酸素剤で、ケイ酸カルシウ
ムの量を活性炭に対して等量販上の範囲とし、しかもケ
イ酸カルシウムと活性炭の合計量が主剤に対して”／＋
〜１の範囲とした脱酸素剤である。<Means for Solving the Problems> The present invention is an oxygen scavenger comprising ascorbic acid or its salt as a main ingredient, an alkali hydroxide compound, primary salt, activated carbon, calcium silicate, and water. The amount of calcium should be within the same commercial range as the amount of activated carbon, and the total amount of calcium silicate and activated carbon should be the same amount as the main ingredient.
It is an oxygen scavenger in the range of 1 to 1.
また、水酸化アルカリ化合物の量は、所期の炭酸ガスの
濃度に応じて定めればよく、また水の量は、他の成分の
合計重量に対して約％であるのが好ましい。Further, the amount of the alkali hydroxide compound may be determined depending on the desired concentration of carbon dioxide gas, and the amount of water is preferably about % based on the total weight of other components.
酸素吸収反応に必要な水は、充填前に予じめ活性炭に吸
着させておいてもよいが、透湿性、防水性の材料で包装
し、使用前にエージングを行い・水を活性炭に吸着させ
るのが好ましい。The water required for the oxygen absorption reaction may be adsorbed on activated carbon in advance before filling, but it should be packaged with moisture-permeable and waterproof material and aged before use to allow the water to be adsorbed on activated carbon. is preferable.
この水を包装する材料としては、例えば内面に熱可塑性
ウレタン層、外面に不織布等の通気性材料を用いればよ
い。As the material for packaging this water, for example, a thermoplastic urethane layer may be used on the inner surface, and a breathable material such as a nonwoven fabric may be used on the outer surface.
く作用〉
脱酸素剤のケイ素カルシウムの量が一定範囲で混合され
ているので粉末タイプであるが、各組成の分散が均一で
あり、また水により固まったり、べたついたりすること
がなく、主剤による酸素吸収速度が速く、同時に炭酸ガ
スの発生速度も早く、短時間に培養雰囲気を達成するこ
とができる。It is a powder type because the amount of silicon calcium in the oxygen scavenger is mixed within a certain range, but the dispersion of each composition is uniform, and it does not harden or become sticky due to water, and the main ingredient The oxygen absorption rate is fast, and at the same time, the carbon dioxide gas generation rate is also fast, and a culture atmosphere can be achieved in a short time.
そして、反応が各部で均一に進むもので経時的にも炭酸
ガス濃度を５〜１０％の範囲に保つことができる。Since the reaction proceeds uniformly in each part, the carbon dioxide concentration can be maintained within the range of 5 to 10% over time.
〈実施例〉
下記表−１からなる組成の脱酸素剤をそれぞれ８ｇずつ
内側が穴あきポリエチレンフィルム、外側が不織布から
なる袋に充填密封した薬剤包装体と内側が熱可塑性ウレ
タン層、外側が不織布からなる袋に水を４ｇずつ充填、
密封した水包装体とをポリ塩化ビニリデンコートポリエ
ステル／ポリエチレンからなる外装体に密封し、４０°
Ｃで３日間エージング後、外装体を開封し、薬剤包装体
のみ取り出して、容Ｉｆｆの容器中にそれぞれ入れ、経
時的に容器中の酸素濃度および炭酸ガス濃度を測定した
。その結果を表−２に示す。<Example> A drug package in which 8 g of each oxygen scavenger having the composition shown in Table 1 below was filled and sealed in a bag made of a perforated polyethylene film on the inside and a nonwoven fabric on the outside, a thermoplastic urethane layer on the inside, and a nonwoven fabric on the outside. Fill each bag with 4g of water,
The sealed water package was sealed in an exterior body made of polyvinylidene chloride coated polyester/polyethylene, and
After aging at C for 3 days, the outer packaging was opened, and only the drug packages were taken out and placed in containers of volume Iff, and the oxygen concentration and carbon dioxide concentration in the containers were measured over time. The results are shown in Table-2.
表−１ 表中の数値は、重量部を示す。Table-1 The numerical values in the table indicate parts by weight.
表−２ 表中の数値は酸素濃度／炭酸ガス濃度を示す。Table-2 The numbers in the table indicate oxygen concentration/carbon dioxide concentration.
〈発明の効果〉
本発明の脱酸素剤は以上の構成からなるので、アスコル
ビン酸またはその塩からなる主剤の酸素吸収反応が均一
、かつ迅速に進み、またこの反応に伴なう主剤の分解反
応も同時に進行し、一定の酸素吸収量、炭酸ガス発生量
を制御できる。<Effects of the Invention> Since the oxygen scavenger of the present invention has the above structure, the oxygen absorption reaction of the main ingredient consisting of ascorbic acid or its salt proceeds uniformly and rapidly, and the decomposition reaction of the main ingredient accompanying this reaction is prevented. At the same time, the amount of oxygen absorbed and the amount of carbon dioxide gas generated can be controlled.
これにより、系内の炭酸ガス濃度を低くしたい場合には
、水酸化アルカリの世を多くすることにより容易に調整
可能で、嫌気性細菌の培養に必要な酸素濃度および炭酸
ガス濃度を２時間以内に得ることができる。As a result, if you want to lower the carbon dioxide concentration in the system, you can easily adjust it by increasing the amount of alkali hydroxide, and the oxygen and carbon dioxide concentrations necessary for culturing anaerobic bacteria can be reduced within 2 hours. can be obtained.
特 許 出 願 人 凸版印刷株式会社 代表者 鈴木和夫Patent applicant Toppan Printing Co., Ltd. Representative: Kazuo Suzuki
Claims (5)
アルカリ化合物、第１鉄塩、ケイ酸カルシウム、活性炭
および水を加えてなる嫌気性細菌培養用脱酸素剤。(1) An oxygen scavenger for anaerobic bacterial culture, comprising ascorbic acid or a salt thereof as a main ingredient, and an alkali hydroxide compound, ferrous salt, calcium silicate, activated carbon, and water.
である第１項記載の嫌気性細菌培養用脱酸素剤。(2) The oxygen absorber for anaerobic bacterial culture according to item 1, wherein the amount of calcium silicate is equal to or more than the amount of activated carbon.
て２／３〜１の範囲である第１項または第２項記載の嫌
気性細菌培養用脱酸素剤。(3) The oxygen absorber for anaerobic bacterial culture according to item 1 or 2, wherein the total amount of calcium silicate and activated carbon is in the range of 2/3 to 1 with respect to the base ingredient.
項乃至第３項のいずれか１項記載の嫌気性細菌培養用脱
酸素剤。(4) The first type in which the amount of water is about 1/2 of the total amount of other components
The oxygen absorber for anaerobic bacterial culture according to any one of items 1 to 3.
る第４項記載の嫌気性細菌培養用脱酸素剤。(5) The oxygen absorber for anaerobic bacterial culture according to item 4, wherein the oxygen absorber is packaged with a water-proof and moisture-permeable material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2697688A JP2666323B2 (en) | 1988-02-08 | 1988-02-08 | Oxygen absorber for anaerobic bacteria culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2697688A JP2666323B2 (en) | 1988-02-08 | 1988-02-08 | Oxygen absorber for anaerobic bacteria culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01202281A true JPH01202281A (en) | 1989-08-15 |
| JP2666323B2 JP2666323B2 (en) | 1997-10-22 |
Family
ID=12208189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2697688A Expired - Fee Related JP2666323B2 (en) | 1988-02-08 | 1988-02-08 | Oxygen absorber for anaerobic bacteria culture |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2666323B2 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992015398A1 (en) * | 1991-02-28 | 1992-09-17 | Toppan Printing Co., Ltd. | Atmosphere conditioning agent |
| US6114162A (en) * | 1997-04-01 | 2000-09-05 | Mitsubishi Gas Chemical Company, Inc. | Atmosphere regulator and method for culturing anaerobic bacteria |
| WO2004033088A1 (en) * | 2002-10-08 | 2004-04-22 | Mitsubishi Gas Chemical Company, Inc. | Oxygen scavenger composition |
| JP2018514214A (en) * | 2015-04-29 | 2018-06-07 | スリーエム イノベイティブ プロパティズ カンパニー | Built-in anaerobic environment generation culture device |
| WO2020261951A1 (en) * | 2019-06-24 | 2020-12-30 | 三菱瓦斯化学株式会社 | Agent for regulating concentration of gas for anaerobic bacterium culture and method for culturing anaerobic bacterium using same |
-
1988
- 1988-02-08 JP JP2697688A patent/JP2666323B2/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992015398A1 (en) * | 1991-02-28 | 1992-09-17 | Toppan Printing Co., Ltd. | Atmosphere conditioning agent |
| AU647460B2 (en) * | 1991-02-28 | 1994-03-24 | Toppan Printing Co. Ltd. | Atmosphere conditioning agent |
| US6114162A (en) * | 1997-04-01 | 2000-09-05 | Mitsubishi Gas Chemical Company, Inc. | Atmosphere regulator and method for culturing anaerobic bacteria |
| WO2004033088A1 (en) * | 2002-10-08 | 2004-04-22 | Mitsubishi Gas Chemical Company, Inc. | Oxygen scavenger composition |
| JPWO2004033088A1 (en) * | 2002-10-08 | 2006-02-09 | 三菱瓦斯化学株式会社 | Oxygen absorber composition |
| JP2018514214A (en) * | 2015-04-29 | 2018-06-07 | スリーエム イノベイティブ プロパティズ カンパニー | Built-in anaerobic environment generation culture device |
| WO2020261951A1 (en) * | 2019-06-24 | 2020-12-30 | 三菱瓦斯化学株式会社 | Agent for regulating concentration of gas for anaerobic bacterium culture and method for culturing anaerobic bacterium using same |
| JP2021000057A (en) * | 2019-06-24 | 2021-01-07 | 三菱瓦斯化学株式会社 | Gas concentration regulator for anaerobic bacterial culture and culture method of anaerobic bacteria using the same |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2666323B2 (en) | 1997-10-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |