JPH01161134A - Analyzing device - Google Patents
Analyzing deviceInfo
- Publication number
- JPH01161134A JPH01161134A JP62318563A JP31856387A JPH01161134A JP H01161134 A JPH01161134 A JP H01161134A JP 62318563 A JP62318563 A JP 62318563A JP 31856387 A JP31856387 A JP 31856387A JP H01161134 A JPH01161134 A JP H01161134A
- Authority
- JP
- Japan
- Prior art keywords
- reaction
- container
- particulate matter
- light
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000006243 chemical reaction Methods 0.000 claims abstract description 43
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 12
- 238000002835 absorbance Methods 0.000 claims abstract description 9
- 239000013618 particulate matter Substances 0.000 claims description 25
- 239000000126 substance Substances 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 abstract description 20
- 239000013307 optical fiber Substances 0.000 abstract description 7
- 239000008187 granular material Substances 0.000 abstract description 5
- 239000000725 suspension Substances 0.000 abstract description 3
- 238000011481 absorbance measurement Methods 0.000 abstract description 2
- 239000012488 sample solution Substances 0.000 abstract description 2
- 238000005070 sampling Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract 6
- 239000000835 fiber Substances 0.000 abstract 2
- 238000003756 stirring Methods 0.000 abstract 2
- 239000003086 colorant Substances 0.000 abstract 1
- 239000011236 particulate material Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 210000005056 cell body Anatomy 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000002238 attenuated effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000013019 agitation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000001747 pupil Anatomy 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は分析装置に関し、特に液体試料中に含有される
極微量成分の定量を自動的に行う分析装置に関−リ−る
ものである。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to an analytical device, and particularly to an analytical device that automatically quantifies trace components contained in a liquid sample. .
［従来の技術］
各種細穴、産業分野においで、試料中の超微量成分分析
の高感度化が望まれている。分析装置の感度が分析目的
とする成分の試料中濃度に対して十分でない場合、当該
成分の濃縮により感度の向上を図ることができる。その
濃縮法の１つとしで、イオン交換樹脂などの粒状物質に
当該成分を吸着させで濃縮する方法かおる。当該成分を
粒状物質に吸着・濃縮させたまま発色させる、あるいは
当該成分を発色させ、発色した成分を粒状物質に吸着・
濃縮させた後、粒状物質層を溶液層より分離し、粒状物
質層の吸光度を直接測定覆ることにより当該成分の定量
を行う方法が報告されている（タランタ（Ｔａｌａｎｔ
ａ）、 ３２．５．３４５ 、１９８５）。[Prior Art] High sensitivity in analyzing ultratrace components in samples is desired in various small holes and in the industrial field. If the sensitivity of the analyzer is not sufficient for the concentration of the component to be analyzed in the sample, the sensitivity can be improved by concentrating the component. One of the concentration methods is to concentrate the components by adsorbing them onto particulate materials such as ion exchange resins. The component can be adsorbed and concentrated on the particulate material to develop color, or the component can be colored and the colored component can be adsorbed and concentrated on the particulate material.
A method has been reported in which the component is quantified by separating the particulate matter layer from the solution layer after concentration and directly measuring the absorbance of the particulate matter layer (Talanta et al.
a), 32.5.345, 1985).
第２〜５図は従来の方法を説明したものでおる。2 to 5 illustrate the conventional method.
第２図に示すように、反応容器８１に所定の液体試料を
入れ、これに所定の反応試薬と粒状物質８２を加え、攪
拌子８３および磁気式攪拌装置８４によって攪拌しなが
ら反応させることにより吸着・濃縮させる。一定時間後
、反応容器８１を静置し、粒状物質を沈降分離させる。As shown in FIG. 2, a predetermined liquid sample is placed in a reaction container 81, a predetermined reaction reagent and a particulate material 82 are added thereto, and the mixture is reacted while being stirred by a stirrer 83 and a magnetic stirring device 84, thereby causing adsorption.・Concentrate. After a certain period of time, the reaction vessel 81 is left still to allow the particulate matter to settle and separate.
次いで第３図に示すように、粒状物質と少量の反応溶液
をスポイト９６で採取し、吸光光度分析用セル本体９１
の試料スペース９５に充填する。なお第４図および第５
図は上記吸光光度分析用セル本体９１、およびこれに粒
状物質８２を充填したところを示す。図中、９２および
９３はスペーリ、９４は細孔である。吸光光度ｈ１に当
該セルを装填することにより粒状物質の吸光度を測定覆
る。Next, as shown in FIG. 3, the particulate matter and a small amount of the reaction solution are collected with a dropper 96 and transferred to the cell body 91 for spectrophotometric analysis.
The sample space 95 is filled. In addition, Figures 4 and 5
The figure shows the cell body 91 for spectrophotometric analysis and the particulate matter 82 filled therein. In the figure, 92 and 93 are spacers, and 94 is a pore. The absorbance of the particulate material is measured by loading the cell to absorbance h1.
以上のように行うことにより、粒状物質に吸着・濃縮さ
せた分析目的成分の定量を行うことができる。By carrying out the procedure described above, it is possible to quantify the component to be analyzed that has been adsorbed and concentrated on the particulate material.
［発明が解決しようとする問題点］
しかしながら第２〜５図に示した従来のバッチ法による
分析では試料ごとにセルユニッ１〜を吸光光度計から着
脱して試料を入れ換えなＣブればならないため、操作か
煩雑で分析の迅速化を図ることが困難でおり、またセル
への粒状物質の充填には多少の熟練を要するという欠点
を有していた。[Problems to be Solved by the Invention] However, in the conventional batch method analysis shown in FIGS. 2 to 5, it is necessary to attach and detach the cell unit 1 to the spectrophotometer for each sample and replace the sample. However, the operation is complicated and it is difficult to speed up the analysis, and filling the cell with particulate matter requires some skill.
本発明の目的はこのような従来技術の欠点を解消し、試
料溶液の採取、試薬との反応、粒状物質への吸着・濃縮
および吸光度測定を全て自動で、かつ半連続的に行うこ
とのできる分析装置を提供することにある。The purpose of the present invention is to overcome these drawbacks of the conventional technology, and to make it possible to automatically and semi-continuously perform sampling of a sample solution, reaction with a reagent, adsorption/concentration to particulate matter, and absorbance measurement. Our objective is to provide analytical equipment.
［問題点を解決するだめの手段］
本発明は、分析対象成分を含む液体試料、前記成分の吸
着・濃縮を行う粒状物質および反応試薬を反応させる、
底部の少なくとも一部が鏡面である反応容器と、前記各
物質を前記反応容器に供給する供給システムと、反応後
、前記粒状物質の吸光度を測定する光源、受光素子およ
び光の導波路を備えた光学測定システムとから構成され
ていることを特徴とする分析装置であり、その反応容器
は、錐状の底部を有していることを好適とするものであ
る。また分析装置には、反応容器への各物質の送液量お
」：び反応時間を制御する制御システムか設（プられて
いることか望ましい。[Means for solving the problem] The present invention involves reacting a liquid sample containing a component to be analyzed, a particulate material that adsorbs and concentrates the component, and a reaction reagent.
A reaction container having at least a part of the bottom having a mirror surface, a supply system for supplying each of the substances to the reaction container, and a light source, a light receiving element, and a light waveguide for measuring the absorbance of the particulate material after the reaction. and an optical measurement system, and the reaction vessel preferably has a conical bottom. It is also desirable that the analyzer be equipped with a control system that controls the amount of each substance sent to the reaction vessel and the reaction time.
１作用コ
本発明ではポンプにより液体試料、反応試薬および分析
成分の吸着・濃縮を行う粒状物質の懸濁液を所定量、反
応容器に送液し、反応容器内で反応させる。反応後、静
置して粒状物質を沈降させ、この粒状物質に光源からの
入射光を反応容器内の光の導波路を経由して入射させる
。粒状物質により減光した光は鏡面になっている反応容
器の底面に反射する。その後、光は別の導波路に入則し
、さらに受光素子に到り、吸光度の測定が行われる。1. In the present invention, a predetermined amount of a suspension of particulate matter for adsorbing and concentrating a liquid sample, a reaction reagent, and an analytical component is delivered to a reaction container by a pump, and reacted within the reaction container. After the reaction, the particulate matter is left to settle, and incident light from a light source is made to enter the particulate matter via a light waveguide in the reaction vessel. The light attenuated by the particulate matter is reflected on the mirrored bottom of the reaction vessel. After that, the light enters another waveguide and reaches a light receiving element, where the absorbance is measured.
また、反応容器の底部を錐状にすると、静置により沈降
した粒状物質は錐状部分に集まり、吸光度の測定か容易
になる。Furthermore, if the bottom of the reaction container is shaped like a cone, the particulate matter that settles out during standing will collect in the cone, making it easier to measure the absorbance.
［実施例］
以下、本発明の実施例について図面を参照して詳細に説
明する。[Example] Hereinafter, an example of the present invention will be described in detail with reference to the drawings.
第１図は本発明の一実施例の概略構成図である。FIG. 1 is a schematic diagram of an embodiment of the present invention.
図中、１は粒状物質８２を収容する粒状物質槽、２は液
体試料槽、３は反応試薬槽、４はトレイン槽である。In the figure, 1 is a particulate matter tank containing particulate matter 82, 2 is a liquid sample tank, 3 is a reaction reagent tank, and 4 is a train tank.
装置の運転開始時、バルブ３１は閉状態にする。When the device starts operating, the valve 31 is closed.
−５＝
液体試料槽２から所定量の液体試料をポンプ１２により
反応容器２１に送液する。粒状物質槽１から粒状物質８
２の懸濁液をポンプ１１により、反応試薬槽３から反応
試薬をポンプ１３によりそれぞれ一定量を反応容器２１
に供給し、攪拌機２５により一定時間攪拌反応させ、分
析成分を粒状物質８２に吸着・濃縮させると共に、吸着
・濃縮した粒状物質８２を発色させる。なお、液体試料
、粒状物質および反応試薬の反応容器への送液量ならび
に反応時間は制御装置（図示せｆ）により制御される。-5= A predetermined amount of liquid sample is sent from the liquid sample tank 2 to the reaction container 21 by the pump 12. Particulate matter tank 1 to particulate matter 8
The suspension of No. 2 is pumped into the reaction container 21 using the pump 11, and the reaction reagent from the reaction reagent tank 3 is pumped into the reaction container 21 using the pump 13.
The sample is supplied to the granular material 82, stirred and reacted for a certain period of time by the stirrer 25, and the analytical components are adsorbed and concentrated in the granular material 82, and the adsorbed and concentrated granular material 82 is colored. Note that the amounts of the liquid sample, particulate matter, and reaction reagent sent to the reaction container and the reaction time are controlled by a control device (f in the figure).
次いで瞳拌を停止し、静置することにより粒状物質８２
を沈降させる。粒状物質８２は反応容器２１の錐状部分
２２の先端部に集まる。Next, the pupil agitation is stopped and the particulate matter 82 is left standing.
to precipitate. The particulate matter 82 collects at the tip of the conical portion 22 of the reaction vessel 21 .
一方、光度計５１の光源５２からの光は光ファイバー４
１を通り、光ファイバー４１の端部から出て、沈降した
粒状物質８２に当てられる。粒状物質８２により吸光、
減光された光は鏡面部分２４により反則され、光ファイ
バー４２の端部より入射し、光ファイバー４２を通って
受光素子５３に入り、吸光度か測定される。On the other hand, the light from the light source 52 of the photometer 51 is transmitted through the optical fiber 4.
1 and exits from the end of the optical fiber 41 and is applied to the settled particulate matter 82. Light absorption by particulate matter 82,
The attenuated light is reflected by the mirror portion 24, enters the end of the optical fiber 42, passes through the optical fiber 42, enters the light receiving element 53, and its absorbance is measured.
−６＝
測定後は、バルブ３１を開状態にし、ポンプ１４により
反応容器２１内の反応溶液の残液を排出口２３がらトレ
イン槽４に排出覆る。-6= After the measurement, the valve 31 is opened, and the residual liquid of the reaction solution in the reaction container 21 is discharged into the train tank 4 through the discharge port 23 by the pump 14 and covered.
バルブ３１を閉じ、ポンプ１２により次の液体試料を供
給し、反応容器を満たし、再びバルブ３１を開き排出す
る。この操作を２〜３度繰り返し、反応容器を洗浄した
後、次の液体試料の測定を上記と同様にして行う。The valve 31 is closed, the pump 12 supplies the next liquid sample to fill the reaction vessel, and the valve 31 is opened again to discharge. After repeating this operation two or three times to wash the reaction vessel, the next liquid sample is measured in the same manner as above.
［発明の効果］
以十詳述したように本発明の分析装置によれば、液体試
料中の分析成分を高感度に分析可能であると共に、自動
的に、かつ半連続的に分析てぎるので、各種研究、産業
分野で用いられている純水、薬液などの液体の晶質管μ
Ｐやυ１水の−Ｅニターの効率化、迅速化および信頼性
の向」−を図ることかできる。[Effects of the Invention] As described in detail above, according to the analyzer of the present invention, the analytical components in a liquid sample can be analyzed with high sensitivity, and can be analyzed automatically and semi-continuously. , crystalloid tubes for liquids such as pure water and chemical solutions used in various research and industrial fields
It is possible to improve the efficiency, speed, and reliability of P and υ1 water.
第１図は本発明の一実施例の概略構成図、第２〜・５図
は従来の分析方式の一例を示づ概８１８構成説明図であ
る、。
１・・・粒状物質槽 ２・・・液体試料櫓３・・・
反応試薬槽 ４・・・トレイン槽１１〜１４・・・
ポンプ ２１．８１・・・反応容器２２・・・皿状
部分 ？３・・・排出口？４・・・鏡面部分
２５・・・攪拌機３１・・・バルブ 旧、
４２・・・光ファイバー５１・・・光度ｈ」５２・・・
光源
５３・・・受光素子 ８２・・・粒状物質８３・
・・攪拌子 ８４・・・磁気式攪拌装置９１・
・・セル本体 ９２．９３・・・スペー１ノ９４
・・・細孔 ９５・・・試料スペース９６・
・・スポイトFIG. 1 is a schematic configuration diagram of an embodiment of the present invention, and FIGS. 2 to 5 are schematic diagrams illustrating an 818 configuration showing an example of a conventional analysis method. 1... Particulate matter tank 2... Liquid sample tower 3...
Reaction reagent tank 4...Train tank 11-14...
Pump 21.81...Reaction vessel 22...Dish-shaped part? 3...Exhaust port? 4... Mirror surface part
25... Stirrer 31... Valve old,
42...Optical fiber 51...Luminous intensity h'52...
Light source 53... Light receiving element 82... Particulate matter 83.
...Stirrer 84...Magnetic stirrer 91.
... Cell body 92.93 ... Space 1 no 94
... Pore 95 ... Sample space 96.
・・Dropper
Claims (3)
濃縮を行う粒状物質および反応試薬を反応させる、底部
の少なくとも一部が鏡面である反応容器と、前記各物質
を前記反応容器に供給する供給システムと、反応後、前
記粒状物質の吸光度を測定する光源、受光素子および光
の導波路を備えた光学測定システムとから構成されてい
ることを特徴とする分析装置。(1) Liquid sample containing the component to be analyzed, adsorption and
A reaction vessel having at least a portion of the bottom having a mirror surface, in which particulate matter to be concentrated and a reaction reagent are reacted; a supply system for supplying each of the substances to the reaction vessel; and after the reaction, measuring the absorbance of the particulate matter. An analysis device comprising an optical measurement system including a light source, a light receiving element, and a light waveguide.
範囲第１項記載の分析装置。(2) The analyzer according to claim 1, wherein the reaction container has a conical bottom.
び反応時間を制御する制御システムか設けられている特
許請求の範囲第１項記載の分析装置。(3) The analyzer according to claim 1, wherein the analyzer is provided with a control system that controls the amount of each substance sent to the reaction container and the reaction time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62318563A JPH01161134A (en) | 1987-12-18 | 1987-12-18 | Analyzing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62318563A JPH01161134A (en) | 1987-12-18 | 1987-12-18 | Analyzing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01161134A true JPH01161134A (en) | 1989-06-23 |
Family
ID=18100529
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62318563A Pending JPH01161134A (en) | 1987-12-18 | 1987-12-18 | Analyzing device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01161134A (en) |
-
1987
- 1987-12-18 JP JP62318563A patent/JPH01161134A/en active Pending
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