JPH01144969A - Bioreactor using hollow fiber - Google Patents
Bioreactor using hollow fiberInfo
- Publication number
- JPH01144969A JPH01144969A JP18350787A JP18350787A JPH01144969A JP H01144969 A JPH01144969 A JP H01144969A JP 18350787 A JP18350787 A JP 18350787A JP 18350787 A JP18350787 A JP 18350787A JP H01144969 A JPH01144969 A JP H01144969A
- Authority
- JP
- Japan
- Prior art keywords
- reaction tube
- hollow fiber
- hollow
- hollow fibers
- bioreactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012510 hollow fiber Substances 0.000 title claims abstract description 52
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 239000000919 ceramic Substances 0.000 claims abstract description 12
- 239000000835 fiber Substances 0.000 claims abstract 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000377 silicon dioxide Substances 0.000 claims description 4
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 8
- 238000009423 ventilation Methods 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 description 18
- 241000196324 Embryophyta Species 0.000 description 7
- 206010020649 Hyperkeratosis Diseases 0.000 description 6
- 239000002609 medium Substances 0.000 description 5
- 244000000626 Daucus carota Species 0.000 description 4
- 235000002767 Daucus carota Nutrition 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000011148 porous material Substances 0.000 description 4
- 210000004102 animal cell Anatomy 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 229920002148 Gellan gum Polymers 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 239000000216 gellan gum Substances 0.000 description 2
- 235000010492 gellan gum Nutrition 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229920002492 poly(sulfone) Polymers 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 229910010293 ceramic material Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000005245 sintering Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229960000344 thiamine hydrochloride Drugs 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/10—Hollow fibers or tubes
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、耐熱性、耐久性を有するセラミックス製の中
空糸を用いた繰返し使用可能なバイオリアクターに関す
る。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a bioreactor that can be used repeatedly and uses heat-resistant and durable ceramic hollow fibers.
従来、植物細胞、動物細胞、微生物などの細胞の培養法
として、寒天などの固形培地を用いる方法、液体培地を
用いて振とう又は撹拌培養する方法があった。また効率
的な方法としてポリスルフォン製、ポリフッ化ビニリデ
ン製などの中空糸モジュールを使用する方法がある。Conventionally, as methods for culturing cells such as plant cells, animal cells, and microorganisms, there have been methods using a solid medium such as agar, and methods of shaking or stirring culture using a liquid medium. Another efficient method is to use a hollow fiber module made of polysulfone, polyvinylidene fluoride, or the like.
しかしながら、細胞の培養技術においては雑菌の混入を
防ぐため、オートクレーブによる滅菌が汎用され、オー
トクレーブ滅菌は一般に１２１℃、１Ｋｇ／ａｎ！、１
５分という条件で行われている。ポリスルフォン製の中
空糸にあっては、２〜３凹のオートクレーブ滅菌により
変色し使用できなくなる欠点があった。また、ポリフッ
化ビニリデン製の中空糸はオートクレーブ滅菌により中
空糸がつぶれる欠点があった。そこで、オートクレーブ
滅菌条件に耐える中空糸を用いたバイオリアクターが求
められていた。However, in cell culture technology, sterilization using an autoclave is commonly used to prevent contamination with germs, and autoclave sterilization is generally performed at 121°C and at a rate of 1 kg/an! ,1
This is done under the condition of 5 minutes. Hollow fibers made of polysulfone have the disadvantage that they become unusable due to discoloration when sterilized in a 2- to 3-concave autoclave. Further, hollow fibers made of polyvinylidene fluoride have the disadvantage that the hollow fibers are crushed by autoclave sterilization. Therefore, there has been a need for a bioreactor using hollow fibers that can withstand autoclave sterilization conditions.
本発明は上記問題を解決することを目的とし、その構成
は、両端を閉塞した反応管に多孔性セラミックスからな
る多数の中空糸を反応管と平行に、両端を反応管端部に
開口させて収納し、該開口部を入口及び出口のそれぞれ
の集合管に導くと共に、上記反応管側壁に中空糸間液供
給口及び中空糸間液排出口を設けたことを特徴とし、更
に上記構成に加えるに中空糸間液供給口及び中空糸間液
排出口に代えて、細菌フィルターを介した通気孔及び排
気孔を設けたことを特徴とする。The present invention aims to solve the above-mentioned problems, and has a structure in which a large number of hollow fibers made of porous ceramics are placed in a reaction tube that is closed at both ends in parallel with the reaction tube, and both ends are opened at the ends of the reaction tube. The openings are guided to the respective collecting pipes of the inlet and outlet, and an inter-hollow fiber liquid supply port and an inter-hollow fiber liquid discharge port are provided on the side wall of the reaction tube, and is further added to the above configuration. The present invention is characterized in that a ventilation hole and an exhaust hole are provided through a bacterial filter in place of the hollow fiber liquid supply port and the hollow fiber liquid discharge port.
本発明に係る中空糸は、内部の培養液が外部に浸出する
ことができるような多孔性のセラミックス素材で、培養
細胞の成長を阻害する有害物質が浸出しない素材であれ
ばよく、例えば、アルミナ、アルミナ−シリカまたはシ
リカなどの焼結体が使用される。The hollow fibers according to the present invention may be made of a porous ceramic material that allows the culture medium inside to leak out to the outside, and that does not leak harmful substances that inhibit the growth of cultured cells, such as alumina. A sintered body such as , alumina-silica or silica is used.
中空糸モジュールはこのような中空糸を、両端を閉塞し
た反応管に反応管と平行に、両端を反応管端部に開口さ
せて収納し、該開口部を入口及び出口のそれぞれの集合
管に導くと共に、上記反応管側壁に中空糸間液供給口及
び中空糸間液排出口を設けたものであり、植物細胞の場
合には中空糸間液供給口及び中空糸間液排出口に代えて
通気孔及び排気孔を設けることもできる。このようなモ
ジュールは中空糸がセラミックス製であるためオートク
レーブ滅菌に耐えることができる。The hollow fiber module stores such a hollow fiber in a reaction tube with both ends closed, parallel to the reaction tube, with both ends opened at the ends of the reaction tube, and the openings are connected to the inlet and outlet collecting tubes. In addition, a hollow fiber liquid supply port and a hollow fiber liquid discharge port are provided on the side wall of the reaction tube, and in the case of plant cells, a hollow fiber liquid supply port and a hollow fiber liquid discharge port are provided instead of Ventilation holes and exhaust holes may also be provided. Since the hollow fibers of such modules are made of ceramic, they can withstand autoclave sterilization.
本発明は植物、動物、微生物など種々の細胞に適用する
ことができ、培養細胞の大きさ、個々の細胞の最適な培
養環境の相違などに従って中空糸の配置間隔を調節する
ことが好ましい。一般に動物細胞では細い中空糸を狭い
間隔を保って配置することが好ましく、微生物細胞では
動物細胞よりやや広く、植物細胞ではカルスの形成を期
待する場合は特に中空糸間の間隙を広くする必要がある
。The present invention can be applied to various cells such as plants, animals, and microorganisms, and it is preferable to adjust the arrangement interval of the hollow fibers according to the size of the cultured cells, differences in the optimal culture environment of individual cells, etc. In general, in animal cells, it is preferable to arrange thin hollow fibers at narrow intervals, in microbial cells it is slightly wider than in animal cells, and in plant cells it is necessary to make the gaps between the hollow fibers particularly wide if callus formation is expected. be.
更に植物細胞の場合は、中空糸間に気体状空気゛を供給
するために、反応管側壁に通気孔及び排気孔を設けるこ
とにより高い増殖効率を期待することができる。反応管
の隅々士で充分な空気を供給するためには、空気供給用
のフィルター付供給孔と排出用のフィルター付排気孔と
を設けることが発泡体被膜、綿栓等細菌の混入を防止で
きるものであればよく孔径が多少大きくとも厚みがあれ
ば遮断可能であり、また素材が疎水性であれば孔径に比
して細菌遮断効果が大きい。一般に孔径は０゜２２μ程
度のものが使用される。Furthermore, in the case of plant cells, high growth efficiency can be expected by providing ventilation holes and exhaust holes on the side wall of the reaction tube in order to supply gaseous air between the hollow fibers. In order to supply sufficient air to every corner of the reaction tube, it is necessary to provide a supply hole with a filter for air supply and an exhaust hole with a filter for discharge to prevent the contamination of bacteria such as foam coatings and cotton plugs. Even if the pore size is somewhat large, it can be blocked as long as it is thick, and if the material is hydrophobic, the bacteria blocking effect will be greater than the pore size. Generally, pores with a diameter of about 0°22μ are used.
また、中空糸表面に、固定化担体を用いて植物細胞を固
定すると連続培養が可能になる。固定化担体としては、
ｋ−カラギナン、アルギン酸、ジェランガム等が使用さ
れる。Furthermore, continuous culture becomes possible by immobilizing plant cells on the surface of the hollow fiber using an immobilization carrier. As an immobilization carrier,
K-carrageenan, alginic acid, gellan gum, etc. are used.
セラミックス製の中空糸を用いる・本発明により耐久性
、耐熱性が向上し、オートクレーブ滅菌に耐えることが
でき、組織培養装置の繰返し使用が可能になった。更に
、植物細胞の場合は通気孔がら気体状空気を供給する本
発明によると、カルス細胞の高い成長速度が得られ、中
空糸の内側と外側が共に液体である従来の方法に比して
５〜１０倍である。Using ceramic hollow fibers - The present invention has improved durability and heat resistance, can withstand autoclave sterilization, and has made it possible to repeatedly use the tissue culture device. Furthermore, in the case of plant cells, according to the present invention, a high growth rate of callus cells is obtained by supplying gaseous air through the vent, compared to the conventional method in which both the inside and outside of the hollow fibers are liquid. ~10 times.
〔実施例１〕
第１図は本発明バイオリアクターを示すフローシートで
あり、第２図は反応管内部の拡大図である。■はパイプ
状の反応管であり、内部に多数のセラミックス製中空糸
２が反応管と平行に配列している。３は反応管の出口で
あり反応管１の〜端を完全に閉塞し、中空糸のみを開口
させて各中空糸内の液体を集合する。集合された中空糸
２内の培養液４は吸引ポンプ５が介在するシリコンチュ
ーブからなる配管６を通過して培養液容器７内に導かれ
る。新しい培養液４は反応管１の他端に設けた出口３と
同一構造の入口８から中空糸２内に供給される。９は反
応管側壁に設けた通気孔であリ、この通気孔９からフィ
ルター１２を介した無菌の空気が供給される。本実施例
では孔径０．２２μのフィルターを用いた。通気孔から
供給された空気は中空糸と中空糸の間の間隙１０を通過
して反応管の通気孔９から離れた側壁に設けた排気孔１
１から排出される。１３は空気調整弁である。中空糸外
壁に付着したカルス細胞は多孔質のセラミックスを介し
て中空糸内部の培養液の供給を受け、通気孔から気体状
空気の供給を受けて効率よく成長する。１４はカルス細
胞である。[Example 1] Fig. 1 is a flow sheet showing the bioreactor of the present invention, and Fig. 2 is an enlarged view of the inside of the reaction tube. 2 is a pipe-shaped reaction tube, inside which a large number of ceramic hollow fibers 2 are arranged parallel to the reaction tube. 3 is the outlet of the reaction tube, and the ends of the reaction tube 1 are completely closed, and only the hollow fibers are opened to collect the liquid in each hollow fiber. The culture solution 4 in the collected hollow fibers 2 passes through a piping 6 made of a silicone tube with a suction pump 5 interposed therebetween, and is led into a culture solution container 7. A new culture solution 4 is supplied into the hollow fiber 2 through an inlet 8 having the same structure as the outlet 3 provided at the other end of the reaction tube 1. Reference numeral 9 denotes a vent hole provided on the side wall of the reaction tube, and sterile air is supplied from this vent hole 9 via a filter 12. In this example, a filter with a pore size of 0.22 μm was used. The air supplied from the vent passes through the gap 10 between the hollow fibers and reaches the exhaust hole 1 provided on the side wall of the reaction tube away from the vent 9.
It is discharged from 1. 13 is an air regulating valve. Callus cells attached to the outer wall of the hollow fibers are supplied with the culture medium inside the hollow fibers through the porous ceramics, and are supplied with gaseous air from the ventilation holes, thereby growing efficiently. 14 is a callus cell.
セラミックス中空糸はアルミナを焼結して製造し、分離
粒子径０．１μ、長さ９０ｍｍ、内径０．５ｍｍ、外径
０．９ｍｍのものを用いた。ニンジン細胞は常法により
ニンジンの肥大根から分離調製した。培地はムラシゲ−
スクーグ培地（Ｍｕｒａｓｈｉｇｅ ＆ Ｓｋｏｏｇ）
にシュークロース３０ ｇ／ｌ、イノシトール１１００
ｒｎ／Ｉｌ、塩酸チアミン０．４ｍｇ／１２．２・４ジ
クロロフエノキシ酢酸とカイネチンをそれぞれ１ｍｇ／
ｌになるように加えた培地を中空糸内に供給した。The ceramic hollow fibers were manufactured by sintering alumina and had a separated particle diameter of 0.1 μ, a length of 90 mm, an inner diameter of 0.5 mm, and an outer diameter of 0.9 mm. Carrot cells were isolated and prepared from enlarged carrot roots by a conventional method. The medium is Murashige
Skoog medium (Murashige & Skoog)
sucrose 30 g/l, inositol 1100
rn/Il, thiamine hydrochloride 0.4 mg/12.2.4 dichlorophenoxyacetic acid and kinetin each 1 mg/
1 of the culture medium was supplied into the hollow fiber.
中空糸表面には０．５％ジェランガムにニンジン細胞培
養液を細胞湿潤重量約１００ｍｇ／ｍｌになるように懸
濁して外側に３ｍｌ流し込み流速約０．５ｍｌ／ｍ ｉ
ｎの条件で培養した。その結果を第１表に示した。On the surface of the hollow fiber, suspend carrot cell culture solution in 0.5% gellan gum to a cell wet weight of approximately 100 mg/ml, and pour 3 ml on the outside at a flow rate of approximately 0.5 ml/m i
The cells were cultured under conditions of n. The results are shown in Table 1.
別に、同一量の同一細胞を同様の培地で液体振とう培養
を行った結果を第１表に併記した。Separately, Table 1 also shows the results of liquid shaking culture of the same amount of the same cells in the same medium.
第 １ 表
本実施例においては、ニンジンカルスが充分に増殖した
後これを分離し、オートクレーブ滅菌をして再度使用を
繰返した。通算１０回オートクレーブ滅菌を繰返したが
中空糸は異常なく使用できた。Table 1 In this example, after the carrot callus had grown sufficiently, it was separated, sterilized in an autoclave, and used again. Autoclave sterilization was repeated 10 times in total, but the hollow fiber could be used without any problems.
図面は本発明の実施例を示し、第１図はバイオリアクタ
ーのフローシート、第２図は反応管内部の拡大図である
。
図面中、符号
１は反応管、２は中空糸、３は出口、４は培養液、６は
配管、８は入口、９は通気孔、１０は間隙、１２はフィ
ルター、１４はカルス細胞である。
特許出願人 エヌオーケー株式会社
代理人 弁理士 吉 １）俊 夫
（外１名）
も１図
ぢ２図
三＝＝＝！The drawings show an embodiment of the present invention, and FIG. 1 is a flow sheet of a bioreactor, and FIG. 2 is an enlarged view of the inside of a reaction tube. In the drawings, 1 is a reaction tube, 2 is a hollow fiber, 3 is an outlet, 4 is a culture medium, 6 is a pipe, 8 is an inlet, 9 is a ventilation hole, 10 is a gap, 12 is a filter, and 14 is a callus cell. . Patent applicant NOK Co., Ltd. Agent Patent attorney Yoshi 1) Toshio (1 other person) Mo 1 Figure 2 Figure 3 ===!
Claims (3)
なる多数の中空糸を反応管と平行に、両端を反応管端部
に開口させて収納し、該開口部を入口及び出口のそれぞ
れの集合管に導くと共に、上記反応管側壁に中空糸間液
供給口及び中空糸間液排出口を設けたことを特徴とする
中空糸を用いたバイオリアクター。(1) A large number of hollow fibers made of porous ceramics are housed in a reaction tube with both ends closed, parallel to the reaction tube, with both ends open at the end of the reaction tube, and the openings are used as a collection of inlets and outlets. A bioreactor using hollow fibers, characterized in that the hollow fibers are guided into a tube, and an interhollow fiber liquid supply port and a hollow fiber liquid discharge port are provided on the side wall of the reaction tube.
なる多数の中空糸を反応管と平行に、両端を反応管端部
に開口させて収納し、該開口部を入口及び出口のそれぞ
れの集合管に導くと共に、上記反応管側壁に細菌フィル
ターを介して通気孔及び排気孔を設けたことを特徴とす
る中空糸を用いたバイオリアクター。(2) A large number of hollow fibers made of porous ceramics are housed in a reaction tube with both ends closed, parallel to the reaction tube, with both ends opened at the end of the reaction tube, and the openings are used as a collection of inlets and outlets. A bioreactor using a hollow fiber, characterized in that a vent hole and an exhaust hole are provided on the side wall of the reaction tube through a bacterial filter.
ミナ−シリカ焼結体またはシリカ焼結体を用いる特許請
求の範囲第２項記載の中空糸を用いたバイオリアクター
。(3) A bioreactor using a hollow fiber according to claim 2, in which an alumina sintered body, an alumina-silica sintered body, or a silica sintered body is used as the porous ceramic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18350787A JPH01144969A (en) | 1987-05-22 | 1987-07-24 | Bioreactor using hollow fiber |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62-123745 | 1987-05-22 | ||
| JP12374587 | 1987-05-22 | ||
| JP18350787A JPH01144969A (en) | 1987-05-22 | 1987-07-24 | Bioreactor using hollow fiber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01144969A true JPH01144969A (en) | 1989-06-07 |
Family
ID=26460592
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18350787A Pending JPH01144969A (en) | 1987-05-22 | 1987-07-24 | Bioreactor using hollow fiber |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01144969A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006119622A1 (en) * | 2005-05-09 | 2006-11-16 | Saxonia Biotec Gmbh. | Apparatus for providing media to cell culture modules |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6043382A (en) * | 1983-08-19 | 1985-03-07 | Ngk Insulators Ltd | Immobilized microorganism and its preparation |
| JPS6125476A (en) * | 1984-07-16 | 1986-02-04 | Teijin Ltd | Cell culture device packed with dispersed hollow fiber |
-
1987
- 1987-07-24 JP JP18350787A patent/JPH01144969A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6043382A (en) * | 1983-08-19 | 1985-03-07 | Ngk Insulators Ltd | Immobilized microorganism and its preparation |
| JPS6125476A (en) * | 1984-07-16 | 1986-02-04 | Teijin Ltd | Cell culture device packed with dispersed hollow fiber |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006119622A1 (en) * | 2005-05-09 | 2006-11-16 | Saxonia Biotec Gmbh. | Apparatus for providing media to cell culture modules |
| US7919307B2 (en) | 2005-05-09 | 2011-04-05 | Alpha Plan Gmbh | Supply system for cell culture module |
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