JP7900392B2 - 高忠実度ＤＮＡポリメラーゼによるｒｈＰＣＲベース増幅産物シーケンシングにおいて、プライマー二量体及びオフターゲット増幅を低減するＲＮアーゼＨ２突然変異体 - Google Patents

高忠実度ＤＮＡポリメラーゼによるｒｈＰＣＲベース増幅産物シーケンシングにおいて、プライマー二量体及びオフターゲット増幅を低減するＲＮアーゼＨ２突然変異体

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JP7900392B2
JP7900392B2 JP2023538971A JP2023538971A JP7900392B2 JP 7900392 B2 JP7900392 B2 JP 7900392B2 JP 2023538971 A JP2023538971 A JP 2023538971A JP 2023538971 A JP2023538971 A JP 2023538971A JP 7900392 B2 JP7900392 B2 JP 7900392B2
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primer
rnase
hybrid
protein
template
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ドボシー，ジョゼフ
フローリグ，ジョン
パーシュバチャー，キャサリン
ベルツ，クリスティン
ローズ，スコット
ベールケ，マーク・アーロン
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インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/09—Recombinant DNA-technology
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
    • C12Y301/26004—Ribonuclease H (3.1.26.4)
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/32—Chemical structure of the sugar
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
JP2023538971A 2020-12-24 2021-12-22 高忠実度ＤＮＡポリメラーゼによるｒｈＰＣＲベース増幅産物シーケンシングにおいて、プライマー二量体及びオフターゲット増幅を低減するＲＮアーゼＨ２突然変異体 Active JP7900392B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063130548P 2020-12-24 2020-12-24
US63/130,548 2020-12-24
US202163277273P 2021-11-09 2021-11-09
US63/277,273 2021-11-09
PCT/US2021/064879 WO2022140553A1 (en) 2020-12-24 2021-12-22 Rnase h2 mutants that reduce primer dimers and off-target amplification in rhpcr-based amplicon sequencing with high-fidelity dna polymerases

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JP2024501832A JP2024501832A (ja) 2024-01-16
JP2024501832A5 JP2024501832A5 (enExample) 2025-01-06
JP7900392B2 true JP7900392B2 (ja) 2026-08-04

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US (2) US12024742B2 (enExample)
EP (1) EP4267762B1 (enExample)
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KR (1) KR20230124946A (enExample)
AU (1) AU2021409723A1 (enExample)
CA (1) CA3206360A1 (enExample)
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Citations (5)

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JP2011521624A (ja) 2008-04-30 2011-07-28 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ
WO2018031625A2 (en) 2016-08-09 2018-02-15 Integrated Dna Technologies, Inc. Rnase h mutants in an emulsion
US20180057868A1 (en) 2016-08-30 2018-03-01 Integrated Dna Technologies, Inc. Cleavable hairpin primers
WO2019215067A1 (en) 2018-05-07 2019-11-14 Roche Innovation Center Copenhagen A/S Massively parallel discovery methods for oligonucleotide therapeutics
WO2020136440A2 (en) 2018-12-28 2020-07-02 National University Of Singapore Methods for targeted complementary dna enrichment

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US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
ATE138106T1 (de) 1988-07-20 1996-06-15 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
AU1239901A (en) 1999-10-29 2001-05-14 Stratagene Compositions and methods utilizing dna polymerases
US20090068643A1 (en) * 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US10760074B2 (en) * 2008-09-03 2020-09-01 Takara Bio Inc. Composition for detection of RNA
AU2014348323B2 (en) * 2013-11-14 2020-09-24 Integrated Dna Technologies, Inc. DNA polymerase mutants having enhanced template discrimination activity
HK1247255A1 (zh) 2014-12-15 2018-09-21 Cepheid 底数大於2的指数核酸扩增

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JP2011521624A (ja) 2008-04-30 2011-07-28 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ
WO2018031625A2 (en) 2016-08-09 2018-02-15 Integrated Dna Technologies, Inc. Rnase h mutants in an emulsion
US20180057868A1 (en) 2016-08-30 2018-03-01 Integrated Dna Technologies, Inc. Cleavable hairpin primers
WO2019215067A1 (en) 2018-05-07 2019-11-14 Roche Innovation Center Copenhagen A/S Massively parallel discovery methods for oligonucleotide therapeutics
WO2020136440A2 (en) 2018-12-28 2020-07-02 National University Of Singapore Methods for targeted complementary dna enrichment

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Title
Front. Microbiol.，2020年，Vol.11, No.560791，pp.1-9
JOURNAL OF BACTERIOLOGY，1998年，Vol.180, No.23，pp.6207-6214

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JP2024501832A (ja) 2024-01-16
CA3206360A1 (en) 2022-06-30
KR20230124946A (ko) 2023-08-28
AU2021409723A9 (en) 2024-09-12
EP4267762B1 (en) 2025-09-17
US20240318237A1 (en) 2024-09-26
AU2021409723A1 (en) 2023-06-22
US20220348997A1 (en) 2022-11-03
ES3060434T3 (en) 2026-03-26
US12024742B2 (en) 2024-07-02
WO2022140553A1 (en) 2022-06-30
EP4267762C0 (en) 2025-09-17
EP4267762A1 (en) 2023-11-01

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