JP7649562B2 - ゲノム編集された細胞の製造方法及びそのためのキット - Google Patents

ゲノム編集された細胞の製造方法及びそのためのキット Download PDF

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JP7649562B2
JP7649562B2 JP2022576241A JP2022576241A JP7649562B2 JP 7649562 B2 JP7649562 B2 JP 7649562B2 JP 2022576241 A JP2022576241 A JP 2022576241A JP 2022576241 A JP2022576241 A JP 2022576241A JP 7649562 B2 JP7649562 B2 JP 7649562B2
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cells
dna
cell
donor dna
sequence
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クヌート ウォルツェン
トーマス ルーク モーリセン
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Kyoto University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
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JP2022576241A 2020-02-19 2020-09-14 ゲノム編集された細胞の製造方法及びそのためのキット Active JP7649562B2 (ja)

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US202062978312P 2020-02-19 2020-02-19
US62/978,312 2020-02-19
PCT/JP2020/035368 WO2021166306A1 (en) 2020-02-19 2020-09-14 Production method of genome-edited cells and kit therefor

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JP2023515907A JP2023515907A (ja) 2023-04-14
JP2023515907A5 JP2023515907A5 (https=) 2023-09-01
JP7649562B2 true JP7649562B2 (ja) 2025-03-21

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US20250290099A1 (en) * 2021-09-02 2025-09-18 The Regents Of The University Of California Materials and methods for targeted genetic manipulations in cells
CN116004542A (zh) * 2022-09-26 2023-04-25 苏州吉纳星辰生物技术有限公司 一种提高基因敲入效率的方法及其应用
CN119836473A (zh) * 2022-10-14 2025-04-15 南京金斯瑞生物科技有限公司 一种提高CRISPR/Cas9介导的基因组敲入编辑效率的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016509063A (ja) 2013-02-25 2016-03-24 サンガモ バイオサイエンシーズ， インコーポレイテッド ヌクレアーゼ媒介性遺伝子破壊を増強するための方法および組成物
JP2019521139A (ja) 2016-07-13 2019-07-25 バーテックス ファーマシューティカルズ インコーポレイテッドＶｅｒｔｅｘ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｉｎｃｏｒｐｏｒａｔｅｄ ゲノム編集効率を高めるための方法、組成物及びキット

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016509063A (ja) 2013-02-25 2016-03-24 サンガモ バイオサイエンシーズ， インコーポレイテッド ヌクレアーゼ媒介性遺伝子破壊を増強するための方法および組成物
JP2019521139A (ja) 2016-07-13 2019-07-25 バーテックス ファーマシューティカルズ インコーポレイテッドＶｅｒｔｅｘ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｉｎｃｏｒｐｏｒａｔｅｄ ゲノム編集効率を高めるための方法、組成物及びキット

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Cold-shock and the mammalian cell cycle."，[online], INTERNET，PUBMED，2002年，PMID 12429927，RIEDER, C.L., et al., CELL CYCLE, 2002, Vol.1, No.3, pp.169-175, [検索日２０２４年８月１４日]
KOLTUN, E.S., et al.，"Discovery of XL413, a potent and selective CDC7 inhibitor."，BIOORGANIC & MEDICINAL CHEMISTRY LETTERS，2012年06月01日，Vol.22, No.11，pp.3727-3731，DOI: 10.1016/j.bmcl.2012.04.024
WAN, L., et al.，"Chemical inactivation of cdc7 kinase in budding yeast results in a reversible arrest that allows efficient cell synchronization prior to meiotic recombination."，GENETICS，2006年12月，Vol.174, No.4，pp.1767-1774，DOI: 10.1534/genetics.106.034303，[Epub 2006 Oct 22]

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WO2021166306A1 (en) 2021-08-26

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