JP7618028B2 - 核酸の増幅に基づく定量化のための方法及び手段 - Google Patents

核酸の増幅に基づく定量化のための方法及び手段 Download PDF

Info

Publication number
JP7618028B2
JP7618028B2 JP2023521681A JP2023521681A JP7618028B2 JP 7618028 B2 JP7618028 B2 JP 7618028B2 JP 2023521681 A JP2023521681 A JP 2023521681A JP 2023521681 A JP2023521681 A JP 2023521681A JP 7618028 B2 JP7618028 B2 JP 7618028B2
Authority
JP
Japan
Prior art keywords
target
competitor
polynucleotide
product
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023521681A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023545097A (ja
JP2023545097A5 (https=
Inventor
ジョン ゲルツ
モリー スティーブンス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ip2ipo Innovations Ltd
Original Assignee
Imperial College Innovations Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial College Innovations Ltd filed Critical Imperial College Innovations Ltd
Publication of JP2023545097A publication Critical patent/JP2023545097A/ja
Publication of JP2023545097A5 publication Critical patent/JP2023545097A5/ja
Priority to JP2024159321A priority Critical patent/JP2025000698A/ja
Application granted granted Critical
Publication of JP7618028B2 publication Critical patent/JP7618028B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/107Reactions characterised by their quantitative nature the purpose being quantitative analysis with a competitive internal standard/control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023521681A 2020-10-08 2021-10-07 核酸の増幅に基づく定量化のための方法及び手段 Active JP7618028B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024159321A JP2025000698A (ja) 2020-10-08 2024-09-13 核酸の増幅に基づく定量化のための方法及び手段

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB2015943.0 2020-10-08
GBGB2015943.0A GB202015943D0 (en) 2020-10-08 2020-10-08 Methods
PCT/GB2021/052594 WO2022074392A1 (en) 2020-10-08 2021-10-07 Methods and means for amplification-based quantification of nucleic acids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2024159321A Division JP2025000698A (ja) 2020-10-08 2024-09-13 核酸の増幅に基づく定量化のための方法及び手段

Publications (3)

Publication Number Publication Date
JP2023545097A JP2023545097A (ja) 2023-10-26
JP2023545097A5 JP2023545097A5 (https=) 2023-11-08
JP7618028B2 true JP7618028B2 (ja) 2025-01-20

Family

ID=73460647

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2023521681A Active JP7618028B2 (ja) 2020-10-08 2021-10-07 核酸の増幅に基づく定量化のための方法及び手段
JP2024159321A Pending JP2025000698A (ja) 2020-10-08 2024-09-13 核酸の増幅に基づく定量化のための方法及び手段

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2024159321A Pending JP2025000698A (ja) 2020-10-08 2024-09-13 核酸の増幅に基づく定量化のための方法及び手段

Country Status (9)

Country Link
US (1) US20230366016A1 (https=)
EP (1) EP4225938B1 (https=)
JP (2) JP7618028B2 (https=)
CN (1) CN117321223A (https=)
AU (1) AU2021356233A1 (https=)
CA (1) CA3195034A1 (https=)
GB (1) GB202015943D0 (https=)
IL (1) IL302008A (https=)
WO (1) WO2022074392A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2635318B (en) 2023-10-20 2026-02-11 Signatur Biosciences Inc Methods and compositions

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140193819A1 (en) 2012-10-31 2014-07-10 Becton, Dickinson And Company Methods and compositions for modulation of amplification efficiency
JP2015535431A (ja) 2012-11-26 2015-12-14 ザ・ユニバーシティ・オブ・トレド 核酸の標準化された配列決定のための方法およびその使用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140193819A1 (en) 2012-10-31 2014-07-10 Becton, Dickinson And Company Methods and compositions for modulation of amplification efficiency
JP2015535431A (ja) 2012-11-26 2015-12-14 ザ・ユニバーシティ・オブ・トレド 核酸の標準化された配列決定のための方法およびその使用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
イメージアナライザーによるDNA検査,医学のあゆみ ,1997年11月22日,Vol. 183, No. 8,p. 525-529

Also Published As

Publication number Publication date
GB202015943D0 (en) 2020-11-25
CN117321223A (zh) 2023-12-29
CA3195034A1 (en) 2022-04-14
EP4225938B1 (en) 2026-04-22
JP2023545097A (ja) 2023-10-26
WO2022074392A1 (en) 2022-04-14
US20230366016A1 (en) 2023-11-16
AU2021356233A1 (en) 2023-05-25
JP2025000698A (ja) 2025-01-07
IL302008A (en) 2023-06-01
EP4225938A1 (en) 2023-08-16

Similar Documents

Publication Publication Date Title
US20220351805A1 (en) Systems and methods for detecting cellular pathway dysregulation in cancer specimens
Teschendorff et al. Statistical and integrative system-level analysis of DNA methylation data
US20230114581A1 (en) Systems and methods for predicting homologous recombination deficiency status of a specimen
Keller et al. Toward the blood-borne miRNome of human diseases
EP3942556A1 (en) Systems and methods for deriving and optimizing classifiers from multiple datasets
Ahmed et al. Early detection of Alzheimer's disease using single nucleotide polymorphisms analysis based on gradient boosting tree
CN107532211B (zh) 把外部生物分子作为标准物质使用的生物分子的分析方法及其试剂盒
CN105339797B (zh) 早期乳腺癌的预后预测诊断用基因标记物及其用途
Maslove et al. Validation of diagnostic gene sets to identify critically ill patients with sepsis
JP2016165286A (ja) 転写物測定値数が減少した、遺伝子発現プロファイリング
Dougherty et al. Genomic signal processing: diagnosis and therapy
US20150100242A1 (en) Method, kit and array for biomarker validation and clinical use
JP2023511368A (ja) 低分子rna疾患分類器
WO2020061072A1 (en) Method of characterizing a neurodegenerative pathology
JP2025000698A (ja) 核酸の増幅に基づく定量化のための方法及び手段
Miglietta et al. Adaptive filtering framework to remove nonspecific and low-efficiency reactions in multiplex digital PCR based on sigmoidal trends
Suar et al. Molecular diagnostics: past, present, and future
Chong et al. SeqControl: process control for DNA sequencing
CN114566224A (zh) 一种用于鉴别或区分不同海拔人群的模型及其应用
Goertz et al. Competitive Amplification Networks enable molecular pattern recognition with PCR
US20260055470A1 (en) Compositions and methods for detecting ovarian cancer
Barnell Bioinformatic tools to alleviate the annotation bottleneck within precision oncology
Waller et al. SPECTRA: Agnostic expression variables for flexible transcriptome modelling in complex disease
Eder et al. STOMP-seq: early multiplexing for high-throughput SMART RNA-sequencing
Kawabata et al. Two‐stage analysis for selecting fixed numbers of features in omics association studies

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230911

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20231030

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20231030

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20231030

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20231205

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240301

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20240514

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240913

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20240927

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20241210

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250107

R150 Certificate of patent or registration of utility model

Ref document number: 7618028

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150