JP7565927B2 - ヒト臍帯間葉系幹細胞シート及びその製造方法 - Google Patents

ヒト臍帯間葉系幹細胞シート及びその製造方法 Download PDF

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JP7565927B2
JP7565927B2 JP2021541040A JP2021541040A JP7565927B2 JP 7565927 B2 JP7565927 B2 JP 7565927B2 JP 2021541040 A JP2021541040 A JP 2021541040A JP 2021541040 A JP2021541040 A JP 2021541040A JP 7565927 B2 JP7565927 B2 JP 7565927B2
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光夫 岡野
キム，キュンソク
グレイガー，デイビッド
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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/51—Umbilical cord; Umbilical cord blood; Umbilical stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115—Platelets, megakaryocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Developmental Biology & Embryology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)
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JP2021541040A 2019-01-16 2020-01-15 ヒト臍帯間葉系幹細胞シート及びその製造方法 Active JP7565927B2 (ja)

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JP2024140602A JP2025000600A (ja) 2019-01-16 2024-08-22 ヒト臍帯間葉系幹細胞シート及びその製造方法

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US201962793199P 2019-01-16 2019-01-16
US62/793,199 2019-01-16
PCT/US2020/013620 WO2020150308A1 (en) 2019-01-16 2020-01-15 Human umbilical cord mesenchymal stem cell sheets and methods for their production

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US (1) US20230357724A1 (enExample)
EP (1) EP3911344A4 (enExample)
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Publication number Priority date Publication date Assignee Title
JP2022155546A (ja) * 2021-03-30 2022-10-13 株式会社カネカ 細胞シートの製造方法
CN115197904B (zh) * 2022-06-22 2024-09-03 广东省科学院生物与医学工程研究所 一种骨修复材料体外评价模型

Family Cites Families (11)

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EP2264148A1 (en) 2000-07-21 2010-12-22 Cellseed Inc. Epidermal cultured cell sheet, multi-layered cultured skin sheet and processes for producing those sheets
EP2267115B8 (en) 2003-02-06 2014-07-23 Cellseed Inc. Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same
WO2004073761A1 (ja) 2003-02-20 2004-09-02 Cellseed Inc. 再生角膜内皮細胞シート、製造方法及びその利用方法
EP1748064B1 (en) 2004-04-25 2019-04-17 CellSeed Inc. Cultured periodontal ligament cell sheet, process for producing the same and method of use thereof
WO2006080434A1 (ja) * 2005-01-27 2006-08-03 Japan Health Sciences Foundation 間葉系幹細胞を含む細胞シート
WO2009105575A1 (en) 2008-02-19 2009-08-27 Weatherford/Lamb, Inc. Expandable packer
WO2010115187A1 (en) * 2009-04-03 2010-10-07 Duke University Methods of making cell sheets, tissue sheets and tissue engineered blood vessels
WO2012131618A1 (en) * 2011-03-30 2012-10-04 Stempeutics Research Private Limited A composition comprising pooled wharton's jelly derived mesenchymal stem cells and methods thereof
DK3321355T3 (da) 2011-12-30 2021-11-08 Amit Patel Fremgangsmåder og sammensætninger til klinisk afledning af en allogen celle og terapeutiske anvendelser
WO2014185517A1 (ja) * 2013-05-17 2014-11-20 テルモ株式会社 シート状細胞培養物の製造方法
CN105647859A (zh) * 2016-03-25 2016-06-08 秦方园 人脐带间充质干细胞与羊膜构建细胞移植片的方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
American Journal of Obstetrics & Gynecology，2019年01月01日，vol.220, no.1，s621
American Society of Nephrology，2018年，Abstract: TH-OR136
Nephrology Dialysis Transplantation，2018年，vol.33, issue suppl_1，page i101, FP209

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WO2020150308A1 (en) 2020-07-23
JP2025000600A (ja) 2025-01-07
EP3911344A1 (en) 2021-11-24
US20230357724A1 (en) 2023-11-09
EP3911344A4 (en) 2022-10-19
JP2022522973A (ja) 2022-04-21

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