JP7349337B2 - Pre-freezing rack and pre-freezing method - Google Patents

Description

The present invention relates to a pre-freezing rack and a pre-freezing method using such a pre-freezing rack.

For example, in the development of new drugs and basic medical research, biological samples (hereinafter simply referred to as "specimens") such as blood, sperm of laboratory animals, fertilized eggs, and cells are used. Samples deteriorate due to biological effects at room temperature. For this reason, it is common to enclose a sample in a flexible bag and freeze it in a cryopreservation device or the like. As a cryopreservation device, a cryopreservation device using liquid nitrogen is widely used because it allows stable storage for a long period of time (see, for example, Patent Documents 1 and 2 below).

By the way, when the above-mentioned sample is cryopreserved at a low temperature of, for example, -150°C or lower, it is known that if the sample at room temperature is rapidly cooled to -150°C, the survival rate of cells decreases. Therefore, before cryopreserving the sample, it is necessary to use a cooling means such as a program freezer, dry ice, or a mechanical freezer to control the cooling rate from room temperature to a predetermined temperature (for example, -80°C). Preliminary freezing of the sealed sample is performed.

Japanese Patent Application Publication No. 2003-128072 Japanese Patent Application Publication No. 2009-136597

By the way, the cryopreservation apparatus described above is required to freeze and preserve a large amount of samples. Therefore, it is possible to use a pre-freezing rack that stores multiple bags side by side, store this pre-freezing rack inside a program freezer, etc., and pre-freeze the samples sealed in each bag all at once. It is being said.

However, since the bag in which the sample is sealed has an uneven thickness, temperature unevenness occurs when the bag is cooled from room temperature to a predetermined temperature, resulting in poor preservation of the sample. Furthermore, when cooling multiple bags at once, temperature unevenness occurs between the bags.

In particular, when the bag is cooled while being stored in a packaging box for distribution, the above-mentioned temperature unevenness becomes more noticeable. On the other hand, if the bag is transferred to a packaging box after pre-freezing, there is concern that the temperature of the bag will rise, leading to a decrease in the quality of the sample and an increase in labor burden.

The present invention was proposed in view of such conventional circumstances, and provides a pre-freezing rack that makes it possible to appropriately pre-freeze biological samples sealed in bags, as well as such a pre-freezing rack. The purpose of the present invention is to provide a preliminary freezing method using a freezing rack.

In order to achieve the above object, the present invention provides the following means.
[1] Pre-freezing used to pre-freeze the biological sample sealed in a flexible bag by cooling the bag from room temperature to a predetermined temperature before cryopreserving the biological sample. A rack for
comprising a holder for holding the bag;
The holder has a pair of holding plates facing each other, and the holder is configured to flexibly deform at least one of the pair of holding plates in a direction in which it is spaced apart from the other holding plate. A pre-freezing rack characterized in that the bag is sandwiched between the pair of holding plates in a state where the holding plates are parallel to each other .
[ 2 ] The pre-freezing rack according to [ 1 ], wherein the holder is made of a sheet metal bent through a bending portion between the pair of holding plates.
[ 3 ] A window portion is provided on the side where the bent portion is provided,
The bag is inserted between the pair of holding plates from the side opposite to the side where the bending part is provided,
The pre-freezing rack according to item [2] above, wherein a jig for pushing out the bag sandwiched between the pair of holding plates is inserted from the window portion.
[ 4 ] The holder according to the above [ 3 ], characterized in that a packaging box for storing the bag is placed over the holder holding the bag from the side opposite to the side where the folding portion is provided. Pre-freezing rack.
[ 5 ] Pre-freezing used to pre-freeze the biological sample sealed in a flexible bag by cooling the bag from room temperature to a predetermined temperature before cryopreserving the biological sample. A rack for
comprising a holder for holding the bag;
The holder includes a pair of holding plates facing each other, a hinge portion rotatably supporting at least one of the pair of holding plates with respect to the other holding plate, and the pair of holding plates. a fixture for fixing the one holding plate to the other holding plate in a state where the holding plates are parallel to each other,
A structure in which the bag is sandwiched between the pair of retaining plates while the one retaining plate is rotated in a direction in which it approaches the other retaining plate, and the pair of retaining plates are parallel to each other. has
A pre-freezing rack characterized in that a packaging box for storing the bag is placed over the holder holding the bag from the side opposite to the side where the hinge portion is provided.
[ 6 ] The pre-freezing rack according to any one of [1] to [ 5 ], wherein the holder is provided with a plurality of the pair of holding plates lined up in opposing directions.
[ 7 ] A pre-freezing method comprising pre-freezing the biological sample sealed in a flexible bag by cooling the bag from room temperature to a predetermined temperature before cryopreserving the biological sample. ,
Using the pre-freezing rack according to any one of [1] to [ 6 ] above,
The biological sample is pre-frozen while cooling the bag by housing the pre-freezing rack inside a cooling device having a cooling function while holding the bag in the holder. Pre-freezing method.

As described above, according to the present invention, there is provided a pre-freezing rack that makes it possible to appropriately pre-freeze a biological sample sealed in a bag, and a pre-freezing method using such a pre-freezing rack. It is possible to provide

The structure of the pre-freezing rack according to the first embodiment of the present invention is shown, in which (a) is a perspective view as seen from the front side, and (b) is a perspective view as seen from the back side. The structure of the holder provided in the pre-freezing rack shown in FIG. (d) is its side view, and (e) is its rear view. FIG. 2 is a sectional view showing a state before a bag is inserted into a holder provided in the pre-freezing rack shown in FIG. 1. FIG. FIG. 4 is a sectional view showing a state in which the bag is inserted into the holder shown in FIG. 3; FIG. 5 is a sectional view showing a state in which the holder shown in FIG. 4 is covered with a packaging box. FIG. 6 is a sectional view showing a state in which the pre-freezing rack shown in FIG. 5 is housed inside the program freezer. FIG. 7 is a sectional view showing a state in which the bag held in the holder shown in FIG. 6 is pushed out by a jig. The structure of the holder provided in the pre-freezing rack according to the second embodiment of the present invention is shown, in which (a) is a perspective view seen from the back side, (b) is a top view, and (c) is a front view. , (d) is its side view, and (e) is its rear view. It is a sectional view showing a state before a bag is held in a holder with which a rack for preliminary freezing concerning a 3rd embodiment of the present invention is provided. 10 is a sectional view showing a state in which the bag is held in the holder shown in FIG. 9. FIG. It is a sectional view showing a state where a bag is held in a holder with which a rack for preliminary freezing concerning a 4th embodiment of the present invention is provided. It is a sectional view showing a state in which a pre-freezing rack according to a fifth embodiment of the present invention is housed inside a program freezer.

Embodiments of the present invention will be described in detail below with reference to the drawings.
Note that the drawings used in the following explanations may show characteristic parts enlarged for convenience in order to make the characteristics easier to understand, and the dimensional ratio of each component may not be the same as the actual one. do not have. Furthermore, the materials, dimensions, etc. exemplified in the following description are merely examples, and the present invention is not necessarily limited thereto, and can be implemented with appropriate modifications within the scope of the gist thereof. .

(First embodiment)
[Pre-freezing rack]
First, as a first embodiment of the present invention, the configuration of a pre-freezing rack (hereinafter simply referred to as "rack") 1A shown in FIGS. 1 to 4, for example, will be described.

Note that FIG. 1 shows the configuration of a rack 1A, in which (a) is a perspective view as seen from the front side, and (b) is a perspective view as seen from the back side. FIG. 2 shows the configuration of the holder 2 included in the rack 1A, in which (a) is a perspective view seen from the back side, (b) is a perspective view seen from the front side, (c) is a front view, ( d) is a side view thereof, and (e) is a rear view thereof. FIG. 3 is a sectional view showing a state before the bag B is inserted into the holder 2 provided in the rack 1A. FIG. 4 is a sectional view showing a state in which the bag B is inserted into the holder 2 shown in FIG. 3.

As shown in FIGS. 1(a) and 1(b), the rack 1A of this embodiment freeze-preserves a biological sample (hereinafter simply referred to as "sample") sealed in a flexible bag B. This is preferably used when the sample is pre-frozen by cooling the bag B from room temperature to a predetermined temperature.

Specifically, this rack 1A includes a plurality of holders 2 that hold bags B, and a rack main body 3 that stores the plurality of holders 2 vertically (heightwise).

As shown in FIGS. 2(a) to 2(e), the holder 2 includes a pair of holding plates 4a, 4b facing each other, and a bent portion 5 bent between the pair of holding plates 4a, 4b. are doing. In addition, in this embodiment, the side where the bending part 5 of the holder 2 is provided will be referred to as the back side of the holder 2, and the opposite side will be described as the front side of the holder 2.

The holder 2 is made of a sheet metal with excellent thermal conductivity, such as aluminum, and is bent between a pair of holding plates 4a and 4b through a bending part 2c, so that the holder 2 has a generally flat rectangular parallelepiped shape as a whole. ing.

The holder 2 has a clip structure in which the bag B is held between a pair of holding plates 4a and 4b facing each other.

Specifically, the pair of holding plates 4a and 4b have shapes that match each other and are formed into a rectangular flat plate shape. The bent portion 5 includes a back plate portion 5a forming the back surface of the holder 2, and a pair of bent portions 5b and 5c bent along the upper and lower edge portions of the back plate portion 5a. There is.

The pair of bent portions 5b and 5c are provided so as to be bent toward the direction in which their distal ends approach each other with respect to the back plate portion 5a. The pair of retaining plates 4a, 4b are bent along the end edges of the pair of bent portions 5a, 5b on the distal end side so as to be in a state facing each other.

Thereby, in the holder 2, it is possible to bend and deform at least one of the pair of holding plates 4a and 4b in a direction in which the holding plate is moved away from the other holding plate. In this embodiment, it is possible to bend and deform the pair of holding plates 4a and 4b in the direction of separating them from each other.

Further, a window portion 6 is provided on the back side of the holder 2. The window portion 6 is provided with a generally rectangular opening extending across the width direction of the back plate portion 5a.

As shown in FIGS. 1(a) and 1(b), the rack body 3 includes a back plate 7a constituting its back surface, a pair of side plates 7b and 7c constituting both sides thereof, and a top plate constituting its upper surface. 7d, and has a frame structure with openings on the front and bottom sides.

The rack body 3 is made of a sheet metal with excellent thermal conductivity, such as aluminum, and is bent between a back plate 7a and a pair of side plates 7b, 7c, and a top plate 7d is attached by welding or the like, so that the entire body is It has a substantially rectangular parallelepiped shape that extends in the vertical direction.

A plurality of holders 2 are housed inside the rack body 3 in a state where they are arranged vertically at equal intervals. Each holder 2 is fixed to the rack body 3 by attaching the back plate portion 5a to the back plate 7a of the rack body 3 by welding or the like. As a result, the holder 2 is provided with a plurality of holders 2 lined up in a direction in which the pair of holding plates 4a and 4b face each other (in the present embodiment, the vertical direction of the rack body 3), with the back side supported in a cantilever manner.

On the back surface of the rack body 3, a plurality of windows 8 are provided vertically in line. The window portion 8 is provided with a substantially rectangular opening extending across the width direction of the back plate 7a so as to coincide with the window portion 6 of each holder 2 described above.

Openings 9 are provided on both sides of the rack body 3. The opening 9 is provided in a substantially rectangular shape extending in the vertical direction of the pair of side plates 7b and 7c.

A handle portion 10 is provided on the upper surface of the rack body 3. The handle portion 10 is attached to the top plate 7d by welding or the like. Thereby, in the rack 1A, it is possible to carry the rack body 3 while holding the handle portion 10.

In the rack 1A of this embodiment having the above configuration, as shown in FIGS. 3 and 4, while inserting the bag B between the pair of holding plates 4a and 4b from the front side of each holder 2, these Bag B is sandwiched between a pair of holding plates 4a and 4b.

At this time, in each holder 2, while bending and deforming the pair of retaining plates 4a, 4b in the direction of separating them from each other, the pair of retaining plates 4a, 4b are parallel to each other. Bag B is sandwiched between them.

Thereby, in the rack 1A of this embodiment, it is possible to hold the bag B by each holder 2 while keeping the thickness of the bag B uniform. Therefore, in the rack 1A of this embodiment, it is possible to suppress the occurrence of temperature unevenness when the bag B is cooled from room temperature to a predetermined temperature.

[Preliminary freezing method]
Next, a preliminary freezing method using the rack 1A will be explained with reference to FIGS. 3 to 7.

Note that FIG. 5 is a sectional view showing a state in which the packaging box C is placed on the holder 2 shown in FIG. 4. FIG. 6 is a sectional view showing the rack 1A shown in FIG. 5 housed inside the program freezer PF. FIG. 7 is a sectional view showing a state in which the bag B held in the holder 2 shown in FIG. 6 is pushed out by the jig T.

In the pre-freezing method using the rack 1A of this embodiment, first, as shown in FIGS. 3 and 4 described above, the bag B is inserted between the pair of holding plates 4a and 4b from the front side of each holder 2. However, each holder 2 holds the bag B by sandwiching the bag B between the pair of holding plates 4a and 4b.

Next, as shown in FIG. 5, the holder 2 holding the bag B is covered with a packaging box C for storing the bag B from the front side. The packaging box C is made of a carton made of paper, resin, etc., in which the bag B is stored after being pre-frozen.

Next, as shown in FIG. 6, the rack 1A is housed inside a program freezer PF, which is a cooling device having a cooling function. Thereby, bag B is cooled from room temperature to a predetermined temperature (for example, -80° C.) while controlling the cooling rate. Thereby, it is possible to pre-freeze the sample sealed in bag B.

Next, as shown in FIG. 7, after taking out the rack 1A housed inside the program freezer PF, the jig T is removed from each window 8 of the rack body 3 and the window 6 of each holder 2. insert. Thereby, it is possible to store the bags B in the packaging box C while pushing out the bags B held in each holder 2 from the front side of the holder 2 using the jig T.

As described above, in the pre-freezing method using the rack 1A of this embodiment, it is possible to hold the bag B by each holder 2 while keeping the thickness of the bag B uniform as described above. It is possible to appropriately pre-freeze the sample sealed in the bag B while suppressing the occurrence of temperature unevenness when cooling from room temperature to a predetermined temperature.

In addition, in the pre-freezing method using the rack 1A of this embodiment, it is possible to quickly and easily transfer the bag B held in the holder 2 to the packaging box C after pre-freezing. It is possible to prevent the quality of the sample from deteriorating due to an increase in temperature.

Furthermore, even if the bag B is stored in a packaging box C with low thermal conductivity such as a paper or resin carton, the sample sealed in the bag B can be quickly cooled and pre-frozen. is possible.

(Second embodiment)
[Pre-freezing rack]
Next, as a second embodiment of the present invention, a pre-freezing rack (hereinafter simply referred to as "rack") 1B shown in FIG. 8, for example, will be described.

8 shows the configuration of the holder 2 included in the rack 1B, in which (a) is a perspective view as seen from the back side, (b) is a top view, (c) is a front view, and (d) is a perspective view of the holder 2. (e) is a side view thereof, and (e) is a rear view thereof. Furthermore, in the following description, the description of parts equivalent to those of the rack 1A will be omitted, and the same reference numerals will be given in the drawings.

As shown in FIGS. 8(a) to 8(e), the rack 1B of this embodiment has basically the same configuration as the rack 1A, except that the shape of the holder 2 is different. That is, the holder 2 included in the rack 1B has only one (in this embodiment, the upper) bent portion 5b that constitutes the bent portion 5.

Further, by omitting the other (lower side in this embodiment) bent portion 5c, one (upper side in this embodiment) retaining plate 4a is moved along the edge on the tip side of one bent portion 5a. The other (lower side in this embodiment) holding plate 4b is bent along the lower edge of the back plate portion 5a.

Thereby, in the holder 2, it is possible to bend and deform one holding plate 4a in a direction away from the other holding plate 4b.

In the rack 1B of this embodiment having the above configuration, the bag B is inserted between the pair of holding plates 4a and 4b from the front side of each holder 2, similarly to the rack 1A shown in FIGS. 3 and 4 above. At the same time, the bag B is sandwiched between the pair of holding plates 4a and 4b.

At this time, in each holder 2, while bending and deforming one of the holding plates 4a, the bag B is sandwiched between the pair of holding plates 4a, 4b in a state where the pair of holding plates 4a, 4b are parallel to each other. The state will be as follows.

Thereby, in the rack 1B of this embodiment, it is possible to hold the bag B by each holder 2 while keeping the thickness of the bag B uniform. Therefore, in the rack 1B of this embodiment, it is possible to suppress the occurrence of temperature unevenness when the bag B is cooled from room temperature to a predetermined temperature.

[Preliminary freezing method]
In the pre-freezing method using the rack 1B of this embodiment, it is possible to hold the bag B by each holder 2 while keeping the thickness of the bag B uniform as in the case of using the rack 1A. Therefore, it is possible to appropriately pre-freeze the sample sealed in bag B while suppressing the occurrence of temperature unevenness when bag B is cooled from room temperature to a predetermined temperature.

In addition, in the pre-freezing method using the rack 1B of this embodiment, it is possible to quickly and easily transfer the bag B held in the holder 2 to the packaging box C after pre-freezing. It is possible to prevent the quality of the sample from deteriorating due to an increase in temperature.

(Third embodiment)
[Pre-freezing rack]
Next, as a third embodiment of the present invention, a pre-freezing rack (hereinafter simply referred to as "rack") 1C shown in FIGS. 9 and 10, for example, will be described.

Note that FIG. 9 is a sectional view showing the configuration of the rack 1C and showing a state before the bag B is held in the holder 2. As shown in FIG. FIG. 10 is a sectional view showing a state in which the bag B is held by the holder 2. As shown in FIG. Furthermore, in the following description, the description of parts equivalent to those of the rack 1A will be omitted, and the same reference numerals will be given in the drawings.

As shown in FIGS. 9 and 10, the rack 1C of this embodiment includes a holder 2 having a hinge structure, whereas the holder 2 provided in the racks 1A and 1B has a clip structure. Other than that, it has basically the same configuration as the rack 1A described above.

That is, the holder 2 included in this rack 1C has a hinge portion 11 located on the back side of the holder 2 and a fixture 12 located on the front side of the holder 2.

The hinge portion 11 rotatably supports one (upper side in this embodiment) holding plate 4a with respect to the other (lower side in this embodiment) holding plate 4b, and supports the pair of holding plates 4a, It is provided along the edge portion on the back side of 4b. The holder 2 is fixed to the rack body 3 by attaching the other holding plate 4b to the rack body 3 by welding or the like.

The fixture 12 is for fixing one retaining plate 4a to the other retaining plate 4b in a state where the pair of retaining plates 4a, 4b are parallel to each other, and is fixed to the front side of the pair of retaining plates 4a, 4b. It consists of a clip that holds the edge of the Note that the fixture 12 is not limited to such a clip, and may be any other fixture that can fix one retaining plate 4a to the other retaining plate 4b.

In the rack 1C of this embodiment having the above configuration, the bag B is inserted between the pair of holding plates 4a and 4b from the front side of each holder 2, similar to the rack 1A shown in FIGS. 3 and 4 above. At the same time, the bag B is sandwiched between the pair of holding plates 4a and 4b.

At this time, in each holder 2, one holding plate 4a is rotated in a direction approaching the other holding plate 4b, and the pair of holding plates 4a and 4b are parallel to each other. The bag B is now sandwiched between the plates 4a and 4b. Further, in this state, one retaining plate 4a is fixed to the other retaining plate 4b by the fixture 12.

Thereby, in the rack 1C of this embodiment, it is possible to hold the bag B by each holder 2 while keeping the thickness of the bag B uniform. Therefore, in the rack 1C of this embodiment, it is possible to suppress the occurrence of temperature unevenness when the bag B is cooled from room temperature to a predetermined temperature.

[Preliminary freezing method]
In the pre-freezing method using the rack 1C of this embodiment, it is possible to hold the bag B by each holder 2 while keeping the thickness of the bag B uniform as in the case of using the rack 1A. Therefore, it is possible to appropriately pre-freeze the sample sealed in bag B while suppressing the occurrence of temperature unevenness when bag B is cooled from room temperature to a predetermined temperature.

In addition, in the pre-freezing method using the rack 1C of this embodiment, it is possible to quickly and easily transfer the bag B held in the holder 2 to the packaging box C after pre-freezing. It is possible to prevent the quality of the sample from deteriorating due to an increase in temperature.

(Fourth embodiment)
[Pre-freezing rack]
Next, as a fourth embodiment of the present invention, a pre-freezing rack (hereinafter simply referred to as "rack") 1D shown in FIG. 11, for example, will be described.

In addition, it is a sectional view showing a state in which the bag B is held in the holder 2 provided in the rack 1D. Furthermore, in the following description, the description of parts equivalent to those of the rack 1A will be omitted, and the same reference numerals will be given in the drawings.

As shown in FIG. 11, the rack 1D of this embodiment includes a holder 2 without the clip structure, whereas the holder 2 provided in the racks 1A and 1B has a clip structure. Other than that, it has basically the same configuration as the rack 1A described above.

That is, the holder 2 included in this rack 1C has only a pair of holding plates 4a and 4b arranged parallel to each other, and has a structure in which the bag B is sandwiched between the pair of holding plates 4a and 4b. There is. The holder 2 is fixed to the rack body 3 while maintaining a clearance for sandwiching the bag B by attaching a pair of holding plates 4a and 4b to the rack body 3 by welding or the like.

In the rack 1D of this embodiment having the above configuration, the bag B is inserted between the pair of holding plates 4a and 4b from the front side of each holder 2, similar to the rack 1A shown in FIGS. 3 and 4 above. At the same time, the bag B is sandwiched between the pair of holding plates 4a and 4b.

At this time, in each holder 2, the pair of holding plates 4a, 4b are parallel to each other, and the bag B is sandwiched between the pair of holding plates 4a, 4b.

Thereby, in the rack 1D of this embodiment, it is possible to hold the bag B by each holder 2 while keeping the thickness of the bag B uniform. Therefore, in the rack 1D of this embodiment, it is possible to suppress the occurrence of temperature unevenness when the bag B is cooled from room temperature to a predetermined temperature.

[Preliminary freezing method]
In the pre-freezing method using the rack 1D of this embodiment, it is possible to hold the bag B by each holder 2 while keeping the thickness of the bag B uniform as in the case where the rack 1A is used. Therefore, it is possible to appropriately pre-freeze the sample sealed in bag B while suppressing the occurrence of temperature unevenness when bag B is cooled from room temperature to a predetermined temperature.

In addition, in the pre-freezing method using the rack 1D of this embodiment, it is possible to quickly and easily transfer the bag B held in the holder 2 to the packaging box C after pre-freezing. It is possible to prevent the quality of the sample from deteriorating due to an increase in temperature.

(Fifth embodiment)
[Pre-freezing rack]
Next, as a fifth embodiment of the present invention, a pre-freezing rack (hereinafter simply referred to as "rack") 1E shown in FIG. 12, for example, will be described.

It is a sectional view showing a state in which the rack 1E is housed inside the program freezer PF. Furthermore, in the following description, the description of parts equivalent to those of the rack 1A will be omitted, and the same reference numerals will be given in the drawings.

As shown in FIG. 12, the rack 1E of this embodiment has a configuration in which a plurality of holders 2 are arranged vertically inside the rack body 3 in the rack 1A, whereas It has a structure in which a plurality of holders 2 are arranged in a row in the width direction inside the holder. Other than that, it has basically the same configuration as the rack 1A described above. That is, a plurality of holders 2 included in this rack 1C are arranged in a line in the direction in which the pair of holding plates 3a and 3b face each other (in the present embodiment, the width direction of the rack body 3).

In this embodiment, the configuration includes the holder 2 having the hinge structure described above, but the holder 2 having the above-described clip structure or the holder 2 having only the pair of retaining plates 4a and 4b may also be used. It may be a configuration.

The rack 1E of this embodiment having the above configuration can be stored in a stacked state inside the program freezer PF.

In addition, in the present invention, "the pair of holding plates become parallel to each other when the bag is sandwiched" is limited to the case where the pair of holding plates 4a and 4b maintain a completely parallel state. However, the pair of retaining plates 4a and 4b may be slightly non-parallel as long as the purpose of keeping the thickness of the back B uniform is achieved.

1A to 1E...Rack for pre-freezing 2...Holder 3...Rack body 4a, 4b...Pair of holding plates 5...Bending part 6...Window part 7a...Back plate 7b, 7c...Pair of side plates 7d...Top plate 8...Window Part 9...Opening part 10...Handle part 11...Hinge part 12...Fixing tool B...Bag C...Packaging box T...Jig

Claims (7)

A pre-freezing rack used to pre-freeze the biological sample by cooling the bag from room temperature to a predetermined temperature before cryopreserving the biological sample sealed in a flexible bag. There it is,
comprising a holder for holding the bag;
The holder has a pair of holding plates facing each other, and the holder is configured to flexibly deform at least one of the pair of holding plates in a direction in which it is spaced apart from the other holding plate. A pre-freezing rack characterized in that the bag is sandwiched between the pair of holding plates in a state where the holding plates are parallel to each other . 2. The pre-freezing rack according to claim 1 , wherein the holder is made of a sheet metal bent through a bending portion between the pair of holding plates. A window portion is provided on the side where the bent portion is provided,
The bag is inserted between the pair of holding plates from the side opposite to the side where the bending part is provided,
3. The pre-freezing rack according to claim 2 , wherein a jig for pushing out the bag sandwiched between the pair of holding plates is inserted through the window. 4. The pre-freezing rack according to claim 3 , wherein a packaging box for storing the bag is placed over the holder holding the bag from a side opposite to the side where the folding part is provided. . A pre-freezing rack used to pre-freeze the biological sample by cooling the bag from room temperature to a predetermined temperature before cryopreserving the biological sample sealed in a flexible bag. There it is,
comprising a holder for holding the bag;
The holder includes a pair of holding plates facing each other, a hinge portion rotatably supporting at least one of the pair of holding plates with respect to the other holding plate, and the pair of holding plates. a fixture for fixing the one holding plate to the other holding plate in a state where the holding plates are parallel to each other,
A structure in which the bag is sandwiched between the pair of retaining plates while the one retaining plate is rotated in a direction in which it approaches the other retaining plate, and the pair of retaining plates are parallel to each other. has
A pre-freezing rack characterized in that a packaging box for storing the bag is placed over the holder holding the bag from the side opposite to the side where the hinge portion is provided. The pre-freezing rack according to any one of claims 1 to 5 , wherein the holder is provided with a plurality of holders lined up in a direction in which the pair of holding plates face each other. A pre-freezing method for pre-freezing a biological sample sealed in a flexible bag by cooling the bag from room temperature to a predetermined temperature before cryopreserving the biological sample, the method comprising:
Using the pre-freezing rack according to any one of claims 1 to 6 ,
The biological sample is pre-frozen while cooling the bag by housing the pre-freezing rack inside a cooling device having a cooling function while holding the bag in the holder. Pre-freezing method.

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