JP7285503B2 - TOTAL PROTEIN MEASUREMENT DEVICE AND TOTAL PROTEIN MEASUREMENT METHOD - Google Patents

Description

TECHNICAL FIELD The present invention relates to a total protein amount measuring device and a total protein amount measuring method.

Patent Literature 1 discloses an apparatus that irradiates blood with light and receives scattered light scattered by the blood to measure the concentration of red blood cells. This device measures the concentration of red blood cells by separately detecting scattered light scattered by blood in a plurality of directions and using the characteristics of each scattered light.
Further, Patent Document 2 discloses an apparatus (blood information measuring apparatus) for measuring the amount of total protein in a sample by the biuret method.

Patent No. 4451567 JP 2004-258025 A

By the way, the apparatus of Patent Document 1 does not specify the amount of total protein.
In addition, since the blood information measuring apparatus of Patent Document 2 uses a measuring method based on the biuret method, it is necessary to specify the amount of total protein after reacting serum or plasma extracted from blood with a reagent. Therefore, in the case of this blood information measuring apparatus, a certain amount of time is required for measurement (time for allowing serum or plasma extracted from blood to react with a reagent). Furthermore, in the case of this blood information measuring apparatus, since light with a wavelength of 400 nm to 750 nm is used as irradiation light, absorption, reflection, scattering, etc. of light by substances not contained in serum, such as hemoglobin, may occur in whole blood. Conceivable. Therefore, in the case of this blood information measuring apparatus, it is considered difficult to measure the absorbance of only protein in whole blood.

The problem to be solved by the present invention is to specify the amount of total protein contained in the blood using the detection results of the light reflected by the blood flowing through the flow channel and the light transmitted through the blood flowing through the flow channel. One example is to provide a total protein measuring device capable of

The invention according to claim 1,
an irradiation unit that irradiates light from an oblique direction to a flow channel for flowing blood;
a first detection unit that detects light emitted by the irradiation unit and reflected by blood flowing through the flow channel;
a second detection unit that detects the light emitted by the irradiation unit and transmitted through the blood flowing through the flow channel;
a specifying unit that specifies the amount of total protein contained in blood using the detection results of the first detection unit and the second detection unit;
It is a total protein amount measuring device comprising

The above-mentioned and other objects, features and advantages will become more apparent with the following preferred embodiments and accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS It is the schematic of the total protein amount measuring apparatus and tube of 1st Embodiment. FIG. 2 is a diagram (cross-sectional view) for explaining the positional relationship between the irradiation unit, the first detection unit, the second detection unit, and the tube, which constitute the total protein amount measuring device of the first embodiment. FIG. 2 is a diagram (perspective view) for explaining the positional relationship between an irradiation unit that constitutes the total protein amount measuring device of the first embodiment and a tube. 1 is a graph showing the relationship between (amount of detected) side scattered light measured using the total protein amount measuring apparatus of the first embodiment and total protein (g/dl). 4 is a graph of calibration curves (iso-Ht lines) of hematocrit values (Ht values) measured using the total protein amount measuring device of the first embodiment. 4 is a table showing the relationship between the amount of total protein for each hetomacrit value (each Ht value) and the amount of corrected reflected light in the case of the first embodiment. It is the graph which made the table of 1st Embodiment two-dimensional. It is a graph when Ht value < less than 25% is excluded from the table of the first embodiment. It is the schematic of the total protein amount measuring apparatus and tube of 2nd Embodiment. Fig. 4 is a graph showing the relationship between (detected amount of) side scattered light and (detected amount of) backscattered light measured using the total protein amount measuring apparatus of the second embodiment, and total protein (g/dl). . 10 is a graph showing iso-Ht lines obtained from the relationship between the corrected reflected light amount (vertical axis) and the logarithmic forward scattered light (horizontal axis) with a base of 2 in the total protein amount measuring apparatus of the second embodiment. . 10 is a table showing the relationship between the amount of total protein for each hetomacrit value (each Ht value) and the corrected reflected light amount in the case of the second embodiment. It is the graph which made the table of 2nd Embodiment two-dimensional. It is a graph when Ht value < less than 25% is excluded from the table of the second embodiment. It is the schematic of the total protein amount measuring apparatus and tube of 3rd Embodiment.

≪Overview≫
Hereinafter, this embodiment (an example of the present invention) will be described by dividing it into first to third embodiments and referring to the drawings in the order in which they are described. In addition, in all the drawings referred to in this specification, the same reference numerals are given to the components having the same functions, and the description thereof will be omitted as appropriate.

<<First embodiment>>
A total protein measuring device 10 (hereinafter referred to as measuring device 10) of the first embodiment will be described below with reference to FIGS. 1 to 8. FIG. First, the function and configuration of the measuring device 10 of this embodiment will be described. Next, the measurement operation of the measurement device 10 of this embodiment will be described. Next, the effects of this embodiment will be described.

<Functions and configuration of the first embodiment>
FIG. 1 is a schematic diagram of a measuring device 10 and a tube TB (an example of a flow path) of this embodiment. After the tube TB is obliquely irradiated with the light L, the measurement apparatus 10 of the present embodiment measures the light L1 reflected by the blood BL flowing through the tube TB and the light L2 transmitted through the blood BL flowing through the tube TB. It has a function of specifying the amount of total protein contained in blood BL using the detection result. As used herein, the term "protein amount" means the total protein amount (g/dL) in 1 dL of serum (plasma).
The measurement device 10 of the present embodiment includes an irradiation section 20, a first detection section 30, a second detection section 40, and a control section 50 (an example of an identification section).
Note that the tube TB is not a component of the measuring device 10 of this embodiment, but is a component that is greatly related to the measuring device 10 . The tube TB is, for example, a cylindrical body having a perfectly circular cross section (see FIGS. 1 to 3), and is made of a transmissive material that transmits the light L emitted by the irradiation unit 20, which will be described later. The tube TB is used as a flow path for the blood BL to flow, and the measurement operation by the measuring device 10 of the present embodiment, which will be described later, is performed by flowing the blood BL into the tube TB from one end side of the tube TB toward the other end side. It is designed to be done in a flowing state.

[Irradiation part]
The irradiation unit 20 is, for example, a laser light source, and has a function of irradiating the blood BL flowing inside the tube TB with light L, as shown in FIG. The irradiation unit 20 is arranged so that the light L emitted by itself has an optical axis LA in a direction oblique to the tube TB, that is, the flow direction (blood flow direction A) of the blood BL flowing through the tube TB. Here, in this embodiment, if the angle of the optical axis LA with respect to the tube TB is an angle θ1, the angle θ1 is, for example, near 90° (90°±5°) and near 45° (45°±5°). is considered an angle. The reason for setting the angle θ1 to such an angle is as follows. The reason for avoiding the angle θ1 near 90° is to facilitate the arrangement of the first detection unit 30, and the reason for avoiding the angle θ1 near 45° is that the first detection unit 30 receives backscattered components. This is to prevent (or make it difficult for) the strong scattered light containing .

[First detection part]
The first detection unit 30 has a function of detecting the light L1 reflected by the blood BL flowing through the tube TB among the light L emitted by the irradiation unit 20 (see FIG. 1). Specifically, the first detection unit 30 detects a component (side scattered component ) is detected.
Note that the first detection section 30 outputs the detected detection signal to the control section 50 .

[Second detector]
The second detection unit 40 has a function of detecting the light L2 transmitted through the blood BL flowing through the tube TB among the light L emitted by the irradiation unit 20 (see FIG. 1). Specifically, the second detection unit 40 detects a component (forward scattering component) transmitted through the blood BL, out of the light L scattered by the red blood cells of the blood BL flowing through the tube TB.
The second detection section 40 outputs the detected detection signal to the control section 50 .

[Positional relationship between irradiation unit, first detection unit and second detection unit]
Next, the positional relationship among the irradiation section 20, the first detection section 30 and the second detection section 40 will be described with reference to FIGS. 1 to 3. FIG. Here, FIG. 2 is a cross-sectional view for explaining the positional relationship between the irradiation section 20, the first detection section 30, the second detection section 40, and the tube TB. FIG. 3 is a perspective view for explaining the positional relationship between the irradiation section 20 and the tube TB.
A two-dot chain line in FIG. 2 indicates an imaginary line M1 that includes the irradiation position (irradiation point) of the light L from the irradiation unit 20 on the tube TB and that is perpendicular to the optical axis LA. Let the two regions separated by the virtual line M1 be the A region and the B region, respectively. Here, if the irradiation unit 20 is arranged in the B region, the first detection unit 30 is arranged in the B region, and the second detection unit 40 is arranged in the A region.

As described above, the first detection unit 30 detects the light L1 reflected by the blood BL flowing through the tube TB among the light L emitted by the irradiation unit 20 (see FIG. 1). As shown in FIGS. 2 and 3, since the first detection unit 30 is arranged in the region B, the first detection unit 30 is arranged at a position where the light L1 reflected by the blood BL can be detected. It can be said that Note that the first detection unit 30 is for detecting the side scattered component of the reflected light L1, as described above. Therefore, the first detection unit 30 is located in the area B in FIGS. 2 and 3 and is located downstream of the irradiation unit 20 in the blood flow direction A (see FIG. 1).
Further, as described above, the second detection unit 40 detects the light L2 transmitted through the blood BL flowing through the tube TB, among the light L emitted by the irradiation unit 20 (see FIG. 1). As shown in FIGS. 2 and 3, since the second detection unit 40 is arranged in the A region, the second detection unit 40 is arranged at a position where the light L2 transmitted through the blood BL can be detected. It can be said that
A virtual plane M2 surrounded by a two-dot chain line in FIG. 3 means a plane formed by the virtual line M1 in FIG.

[Control part]
Next, the control unit 50 of this embodiment will be described. The control unit 50 has a function of controlling the irradiation unit 20 , the first detection unit 30 and the second detection unit 40 . Further, the control unit 50 detects and outputs detection signals from the first detection unit 30 and the second detection unit 40, that is, the detection signals of the first detection unit 30 and the second detection unit 40 during the measurement operation described later. It has a function of specifying the amount of total protein contained in blood BL using the results.
The specific functions of the control unit 50 of this embodiment will be explained later in the description of the measurement operation of this embodiment.

The above is the description of the measuring device 10 of the present embodiment.

<Measurement operation of the first embodiment>
Next, a measurement operation using the measurement device 10 of the first embodiment will be described mainly with reference to FIGS. 4 to 8. FIG. In the following, an overview of the measurement operation is first described, and then the specific mechanism of the amount of total protein in the measurement operation is described.

[Outline of measurement operation]
First, when the measurer turns on the measurement start switch (not shown) of the measuring device 10, control by the control section 50 is started. Along with this, the control unit 50 operates a tube pump (not shown) attached to the tube TB to flow the blood BL through the tube TB.

Next, the control unit 50 operates the irradiation unit 20 , the first detection unit 30 and the second detection unit 40 . Along with this, the irradiation unit 20 irradiates the light L toward the blood BL flowing through the tube TB (see FIG. 1). A part of the light L (light L1) irradiated to the blood BL is reflected by the blood BL and detected by the first detection unit 30 . That is, the first detection unit 30 detects the side scattered component of the light L emitted by the irradiation unit 20 and scattered by the blood BL. The first detector 30 then outputs the detected detection signal to the controller 50 .
In addition, the remaining part of the light (light L<b>2 ) with which the blood BL is irradiated is transmitted through the blood BL and detected by the second detection unit 40 . That is, the second detection unit 40 detects the forward scattered component of the light L emitted by the irradiation unit 20 and scattered by the blood BL. The second detector 40 then outputs the detected detection signal to the controller 50 .

Then, the control unit 50, to which the detection signals respectively output by the first detection unit 30 and the second detection unit 40 are input, uses the graph shown in FIG. 7 or the graph shown in FIG. Determine the amount of total protein.
Here, the graph of FIG. 7 is a two-dimensional graph of a table (data) showing the relationship between the detection results of the first detection unit 30 and the second detection unit 40 and the amount of total protein contained in the blood BL. . The graph in FIG. 7 consists of the above detection results, that is, the output of the forward scatter component, which is the detection result of the second detection unit 40, and the output of the side scatter component, which is the detection result of the first detection unit 30. It is composed of a plurality of polygonal lines A to H. In this graph, the amount of total protein on each line (lines AH) is assumed to be the same for each line. That is, the control unit 50 specifies the amount of total protein contained in the blood BL using the detection results of the first detection unit 30 and the second detection unit 40 and the graph (table) of FIG. The graph of FIG. 8 is a graph obtained by excluding the portion where the hematocrit value (Ht value) is less than 25% from the graph of FIG.
The tables represented by the graphs of FIGS. 7 and 8 are stored as data in a storage device (not shown) of the control unit 50. FIG.

The above is the description of the outline of the measurement operation.

[Specific mechanism of the amount of total protein in the measurement operation]
Next, the specific mechanism of the amount of total protein in the measurement operation will be explained.

FIG. 4 is a graph showing the relationship between (amount of detected) side scattered light measured using the measuring device 10 of the present embodiment and the amount of total protein (g/dl), plotted by Ht value. 4 approximate lines for each Ht value of the value obtained by From the graph of FIG. 4, the inventors of the present application assume that each approximation line has a constant slope (that is, if it is assumed that total protein and side scattered light have a linear function relationship ), derived that the amount of a given total protein and the side scattered light have a corresponding relationship. In other words, the inventors of the present application have derived that when the amount of total protein contained in blood BL is a predetermined amount, side scattered light is detected at a predetermined detection amount. In other words, the inventors of the present application have derived that the amount of total protein contained in the blood BL is detected at a predetermined amount when the side scattered light is detected at a predetermined detection amount.
The horizontal axis in the graph of FIG. 4 is the value of logarithm of total protein (g/dl) with 2 as the base.

The graph of FIG. 5 is a graph of a calibration curve (iso-Ht line) of hematocrit values (Ht values) measured using the measuring device 10 of the present embodiment. Line I, line II, line III and line IV in FIG. 5 are for Ht values of 55%, 40%, 25% and 10%, respectively. Further, the table of FIG. 6 is a table showing the relationship of the corrected side scattered light (reflected light) corresponding to the amount of total protein (TP) for each hetomacrit value (each Ht value). The table in FIG. 6 is derived from the results in FIG.

The amount of total protein (TP) for each hetomacrit value (each Ht value) in this embodiment is derived by the following (Equation 1).

(Formula 1) TP={(Ya×XY _ref )/Y _dif }+TP _ref

Here, each parameter of (Formula 1) is as follows.
X: log2 obtained by measurement (detection output of the second detection unit 40)
Y: Detected output of first detection unit 30 obtained by measurement a: Inclination of detected amount TP _ref : Reference total protein Y _ref : Intercept of standard curve of reference total protein Y _dif : Y value when total protein increases by 1 difference

The graph in FIG. 7 is polygonal lines A to H obtained by plotting the corrected side scattered light (reflected light) values in the table in FIG. 6 on the graph in FIG. 5 and connecting the points with lines for each total protein. . Lines A to H show standard curves for each total protein.
Then, in the measurement operation of the present embodiment, the control unit 50 to which the detection signals respectively output by the first detection unit 30 and the second detection unit 40 are input specifies the amount of total protein based on the mechanism described above.

The above is a description of the specific mechanism of the amount of total protein in the measurement operation. The above is the description of the measurement operation of the present embodiment.

<Effects of the first embodiment>
Next, the effects of this embodiment will be described.
As described in the measurement operation of the present embodiment, the measurement apparatus 10 of the present embodiment uses the detection results of the light L1 reflected by the blood BL flowing through the tube TB and the light L2 transmitted through the blood BL flowing through the tube TB. , can determine the amount of total protein contained in blood BL.
In addition, the measurement apparatus 10 of the present embodiment uses the detection results of the first detection unit 30 and the second detection unit 40 and the data prepared in advance (the table that is the basis of the graph of FIG. 7 or FIG. 8). can identify the amount of total protein contained in blood BL.

As described above, the graph in FIG. 8 is obtained by excluding the portion where the hematocrit value (Ht value) is less than 25% from the graph in FIG. Ht values less than 25% are rare values for human blood BL. Therefore, when the object to be examined by the measuring apparatus 10 of the present embodiment is human blood BL, the graph of FIG. 8 (the table on which the graph of FIG. 8 is based) may be used. In this case, that is, when using the graph of FIG. 8, it can be said to be advantageous in that the calculation for specifying the amount of total protein becomes easier than when using the graph of FIG. Further, from FIGS. 7 and 8, the portion where the Ht value is less than 25% has a large change rate (steep gradient) of the side scattered light with respect to the forward scattered light. Therefore, it can be said that this portion is greatly affected by measurement errors and the like. Therefore, when using the graph of FIG. 8, it can be said that it is advantageous also from this point.

The above is the description of the effects of the present embodiment. Also, the above is the description of the first embodiment.

<<Second embodiment>>
Next, the total protein amount measuring device 10A (hereinafter referred to as measuring device 10A, see FIG. 9) of the second embodiment will be described. Only parts of this embodiment that differ from the first embodiment (see FIG. 1) will be described below. Moreover, in the following description, when using the same component as the component of 1st Embodiment in this embodiment, suppose that the same code|symbol as the component of 1st Embodiment is used.

<Functions and configuration of the second embodiment>
FIG. 9 is a schematic diagram of the measuring device 10A and the tube TB (an example of the flow path) of this embodiment. 10 A of measuring apparatuses of this embodiment are further equipped with the 3rd detection part 60 with respect to the structure of the measuring apparatus 10 of 1st Embodiment.
Here, the third detection unit 60 is arranged at a position different from the first detection unit 30 in the B area (see FIGS. 2 and 3), and the light L emitted by the irradiation unit 20 is reflected by the blood BL. It has a function of detecting light L3. Specifically, the third detection unit 60 detects a component (backscatter component) scattered in the direction in which the irradiation unit 20 is located, out of the light L scattered by the red blood cells of the blood BL flowing through the tube TB. It has become. Here, the direction in which the irradiation unit 20 is positioned is the irradiation unit 20 side of the one-dot chain line B (virtual straight line B drawn radially from the intersection of the optical axis LA and the center of the tube TB) in FIG. means.
Note that the third detection section 60 outputs the detected detection signal to the control section 50 .
Further, the control unit 50 of the present embodiment uses the detection results of the first detection unit 30, the second detection unit 40, and the third detection unit 60 to specify the amount of total protein contained in the blood BL. ing.
The above is the description of the function and configuration of the measuring apparatus 10A of the present embodiment.

<Measurement operation of the second embodiment>
Next, an operation for specifying the amount of total protein using the measuring device 10A of this embodiment will be described mainly with reference to FIGS. 10 to 14. FIG.

In the case of this embodiment, the control unit 50 uses the detection outputs of the first detection unit 30, the second detection unit 40, and the third detection unit 60 and the graph shown in FIG. 13 or the graph shown in FIG. Determine the amount of total protein contained in blood BL.
Here, the graph in FIG. 13 is a two-dimensional table showing the relationship between the detection results of the first detection unit 30, the second detection unit 40, and the third detection unit 60 and the amount of total protein contained in the blood BL. graph. The graph of FIG. 13 shows the output of the forward scatter component, which is the detection result of the second detection unit 40, and the output of the side scatter component, which is the detection result of the first detection unit 30, which is the detection result described above. 3 values obtained from the output of the backscattered light component, which is the detection result of the detection unit 60 (hereinafter referred to as specific values). In this graph, the amount of total protein on each line (lines AH) is assumed to be the same for each line. That is, the control unit 50 uses the detection results of the first detection unit 30, the second detection unit 40, and the third detection unit 60 and the graph (table) of FIG. 13 to determine the amount of total protein contained in the blood BL. identify. The graph in FIG. 14 is obtained by excluding the portion where the hematocrit value (Ht value) is less than 25% from the graph in FIG.
The tables represented by the graphs of FIGS. 13 and 14 are stored as data in a storage device (not shown) of the control unit 50. FIG.

Next, the specific mechanism of the amount of total protein in the measurement operation of this embodiment will be described.
FIG. 10 shows specific values obtained from (detected amount of) side scattered light and (detected amount of) measured using the measuring device 10A of the present embodiment, and the amount of total protein (g / dl) and It is a graph showing the relationship between and four approximation lines for each Ht value of values plotted for each Ht value. From the graph of FIG. 10, the inventors of the present application assume that each approximate line has a constant slope (that is, assuming that the total protein and the specific value have a linear function relationship), It was derived that there is a corresponding relationship between a given total protein amount and a specific value. In other words, the inventors of the present application have derived that a predetermined specific value is obtained when the amount of total protein contained in blood BL is a predetermined amount. In other words, the inventors of the present application have found that when the side scattered light and the backscattered light are each detected at a predetermined detection amount, the amount of total protein contained in the blood BL is detected at a predetermined amount. derived.
The horizontal axis in the graph of FIG. 10 is the value of logarithm of total protein (g/dl) with 2 as the base.

The graph of FIG. 11 is a graph of a calibration curve (iso-Ht line) of hematocrit values (Ht values) measured using the measuring device 10A of the present embodiment. Line I, line II, line III and line IV in FIG. 11 are for Ht values of 55%, 40%, 25% and 10%, respectively. The table of FIG. 12 is a table showing the relationship of the corrected side scattered light (reflected light) corresponding to the amount of total protein (TP) for each hetomacrit value (each Ht value). The table in FIG. 12 is derived from the results in FIG.

Here, the amount of total protein (TP) for each hetomacrit value (each Ht value) in this embodiment is derived from (Formula 1) in the first embodiment. However, in the case of this embodiment, each parameter is as follows.
X: log2 obtained by measurement (detection output of the second detection unit 40)
Y: A×(detection output of the first detection unit 30 obtained by measurement)+B×(detection output of the third detection unit 60 obtained by measurement)
A: Multiplication coefficient B: Multiplication coefficient a: Inclination of detected amount TP _ref : Reference total protein Y _ref : Intercept of standard curve of reference total protein Y _dif : Y value difference when total protein increases by 1

The graph in FIG. 13 is polygonal lines A to H obtained by plotting the corrected side scattered light (reflected light) values in the table in FIG. 12 on the graph in FIG. 11 and connecting the points with lines for each total protein. . Lines A to H show standard curves for each total protein.
Then, in the measurement operation of the present embodiment, the control unit 50 to which the detection signals respectively output by the first detection unit 30, the second detection unit 40, and the third detection unit 60 are input, measures the total protein based on the mechanism described above. Identify quantity.

The above is the description of the specific mechanism of the amount of total protein in the measurement operation of this embodiment. The above is the description of the measurement operation of the present embodiment.

<Effects of Second Embodiment>
Next, the effects of this embodiment will be described.
As described in the measurement operation of the present embodiment, the measurement apparatus 10 of the present embodiment detects the light L1 and L3 reflected by the blood BL flowing through the tube TB and the light L2 transmitted through the blood BL flowing through the tube TB. can be used to determine the amount of total protein contained in blood BL.
In addition, the measurement apparatus 10 of the present embodiment includes the detection results of the first detection unit 30, the second detection unit 40, and the third detection unit 60, and the data prepared in advance (which is the basis of the graph of FIG. 13 or FIG. 14). table), the amount of total protein contained in blood BL can be specified.
The effect of the graph of FIG. 14 on the graph of FIG. 13 is the same as that of the graph of FIG. 8 on the graph of FIG. 7 in the first embodiment.

In addition, in the case of the present embodiment (see FIGS. 13 and 14), the plurality of polygonal lines A to H are flat between line I and line II, whereas in the case of the first embodiment (FIGS. 7 and 8 ) is different. Therefore, in the case of the present embodiment, compared with the first embodiment, it can be said that the processing required for specifying the amount of total protein can be simplified, and the amount of total protein can be specified with high accuracy.

The above is the description of the effects of the present embodiment. The above is the description of the second embodiment.

<<Third Embodiment>>
Next, the total protein amount measuring device 10B (hereinafter referred to as measuring device 10B, see FIG. 15) of the third embodiment will be described. Only parts of this embodiment that differ from the first embodiment (see FIG. 1) and the second embodiment (see FIG. 9) will be described below. Further, in the following description, when the same constituent elements as those of the first and second embodiments are used in this embodiment, the same reference numerals as those of the first and second embodiments are used.

<Functions and configuration of the third embodiment>
FIG. 15 is a schematic diagram of the measuring device 10B and tube TB of this embodiment. The measuring device 10B of the present embodiment has the following configuration as compared with the measuring device 10A of the second embodiment. That is, the second detection unit 40 and the third detection unit 60 are arranged upstream in the blood flow direction A with respect to the irradiation unit 20 (an example of the first irradiation unit) and the first detection unit 30 . Furthermore, in the case of the present embodiment, the light source of the light L2 and L3 detected by the second detection unit 40 and the third detection unit 60 is the newly provided irradiation unit 22 (an example of the second irradiation unit). is the light LB emitted by . Here, the irradiating section 22 irradiates the tube TB with the light LB from an oblique direction at an incident position different from the incident position of the light L irradiated by the irradiating section 20 on the tube TB.
Further, in the case of the present embodiment, as an example, the irradiation unit 20 and the irradiation unit 22 emit different wavelengths of light L and LB. For example, the irradiation section 20 emits light with a wavelength of 822 nm, and the irradiation section 22 emits light with a wavelength of 795 nm.
The above is the description of the function and configuration of the measuring device 10B of the present embodiment.

<Measurement operation of the third embodiment>
The measurement operation of this embodiment is the same as that of the second embodiment.

<Effects of the third embodiment>
The measuring device 10B of this embodiment has the same effects as those of the second embodiment.
Further, in the case of the present embodiment, as shown in FIG. 15, the first detection section 30 and the third detection section 60 detect the lights L1 and L3 scattered at different positions. Further, in the case of the present embodiment, the wavelength of the light L that is the source of the light L1 detected by the first detection unit 30 and the wavelength of the light LB that is the source of the light L3 detected by the third detection unit 60 are different. different. Therefore, unlike the second embodiment, the present embodiment can measure the enzyme saturation level of blood BL, which is an advantage that the second embodiment does not have. Along with this, in the case of this embodiment, it is possible to correct scattering and absorption due to changes in red blood cell volume due to differences in enzyme saturation. In addition, in the case of this embodiment, since the enzyme saturation can be measured, the influence of scattering due to the enzyme saturation can be eliminated (or less likely to be affected) when blood components are measured.
Furthermore, in the case of the present embodiment, it is possible to measure blood components in whole blood with flow velocity, and obtain real-time measurement results (measurement results that change over time).

The above is the description of the effects of the present embodiment. The above is the description of the third embodiment.

As described above, the present invention has been described by taking the first to third embodiments as examples, but the present invention is not limited to these embodiments. The technical scope of the present invention includes, for example, the following forms (modifications).

For example, in the description of each embodiment, as an example, the tube TB is assumed to be a cylindrical body having a perfectly circular cross section (see FIGS. 1 to 3, etc.). However, the shape of the tube TB does not have to be a cylindrical body with a perfectly circular cross section. For example, a surface may be formed with respect to the light irradiation position.

Also, the irradiation units 20 and 22 have been described as laser light sources. However, for example, an LED light source may be used instead of the laser light source.

In addition, although the control unit 50 uses a table to specify the amount of total protein, the table is based on the concept of multiplying the detection results of the detection units 30, 40, and 60 by a predetermined coefficient. You can change it. That is, any data may be used.

In the third embodiment, the irradiation section 20 emits light with a wavelength of 822 nm, and the irradiation section 22 emits light with a wavelength of 795 nm. Further, for example, one of the wavelengths of the light emitted by the irradiation unit 20 and the irradiation unit 22 may be red light, and the other may be infrared light.

Further, in the third embodiment, as an example, the wavelengths of the lights L and LB emitted by the irradiation unit 20 and the irradiation unit 22 are different, but the wavelengths may be the same.

Further, in the third embodiment, the second detection unit 40 is moved to the upstream side in the blood flow direction A from the first detection unit 30 based on the second embodiment, but the second detection unit 40 is moved. It doesn't have to be.
Examples of reference forms are added below.
1. an irradiation unit that irradiates light from an oblique direction to a flow channel for flowing blood;
a first detection unit that detects light emitted by the irradiation unit and reflected by blood flowing through the flow channel;
a second detection unit that detects the light emitted by the irradiation unit and transmitted through the blood flowing through the flow channel;
a specifying unit that specifies the amount of total protein contained in blood using the detection results of the first detection unit and the second detection unit;
total protein amount measuring device.
2. The specifying unit specifies the amount of total protein contained in blood using data indicating the relationship between the detection result and the amount of total protein contained in blood.
1. The total protein amount measuring device according to .
3. an irradiation unit that irradiates light from an oblique direction to a flow channel for flowing blood;
a first detection unit that detects light emitted by the irradiation unit and reflected by blood flowing through the flow channel;
a second detection unit that detects the light emitted by the irradiation unit and transmitted through the blood flowing through the flow channel;
a third detection unit arranged at a position different from the first detection unit and configured to detect the light emitted by the irradiation unit and reflected by the blood flowing through the flow channel;
a specifying unit that specifies the amount of total protein contained in blood using the detection results of the first detecting unit, the second detecting unit, and the third detecting unit;
total protein amount measuring device.
4. a first irradiation unit that irradiates light from an oblique direction to a flow path for flowing blood;
a first detection unit that detects the light emitted by the first irradiation unit and reflected by the blood flowing through the flow channel;
a second irradiating unit that irradiates the flow path with light from an oblique direction at an incident position in the flow path that is different from the incident position of the light irradiated by the first irradiating unit;
a second detection unit that detects the light emitted by the second irradiation unit and transmitted through the blood flowing through the flow channel;
a third detection unit that detects the light emitted by the second irradiation unit and reflected by the blood flowing through the flow channel;
a specifying unit that specifies the amount of total protein contained in blood using the detection results of the first detecting unit, the second detecting unit, and the third detecting unit;
total protein amount measuring device.
5. The wavelength of the light emitted by the second irradiation unit is different from the wavelength of the light emitted by the first irradiation unit.
4. The total protein amount measuring device according to .
6. The specifying unit specifies the amount of total protein contained in blood using data indicating the relationship between the detection result and the amount of total protein contained in blood.
3. ~ 5. Total protein amount measuring device according to any one of.
7. irradiating light from an irradiating part in an oblique direction to a flow path for flowing blood,
A first detection unit detects the light emitted by the irradiation unit and reflected by the blood flowing through the flow channel,
A second detection unit detects the light emitted by the irradiation unit and transmitted through the blood flowing through the flow channel,
Using the detection results of the first detection unit and the second detection unit to identify the amount of total protein contained in the blood by the identification unit;
Total protein amount measurement method.
8. irradiating light from an irradiating part in an oblique direction to a flow path for flowing blood,
A first detection unit detects the light emitted by the irradiation unit and reflected by the blood flowing through the flow channel,
A second detection unit detects the light emitted by the irradiation unit and transmitted through the blood flowing through the flow channel,
A third detection unit arranged at a position different from the first detection unit detects light emitted by the irradiation unit and reflected by blood flowing through the flow channel,
Using the detection results of the first detection unit, the second detection unit, and the third detection unit, the amount of total protein contained in the blood is specified by the specifying unit.
Total protein amount measurement method.
9. irradiating light from a first irradiation unit obliquely to a flow channel for flowing blood;
Detecting light emitted by the first irradiation unit and reflected by the blood flowing through the flow path by the first detection unit,
irradiating a second irradiation unit with light from an oblique direction to the flow channel at an incident position different from the incident position of the light irradiated by the first irradiation unit in the flow channel;
A second detection unit detects the light emitted by the second irradiation unit and transmitted through the blood flowing through the flow channel,
Detecting light emitted by the second irradiation unit and reflected by the blood flowing through the flow path by a third detection unit,
Using the detection results of the first detection unit, the second detection unit, and the third detection unit, the amount of total protein contained in the blood is specified by the specifying unit.
Total protein amount measurement method.

Claims (2)

a first irradiation unit that irradiates light in a direction oblique to a blood flow direction of the flow channel to a flow channel through which blood flows;
By the blood irradiated from the first irradiation unit and flowing through the flow channel, the region on the side where the first irradiation unit is arranged among the regions facing each other through the flow channel in the radial direction of the flow channel. a first detection unit that detects scattered light;
The side opposite to the side on which the first irradiation unit is arranged, among the regions facing each other via the flow channel in the radial direction of the flow channel, due to the blood irradiated from the first irradiation unit and flowing through the flow channel. a second detection unit that detects light scattered in the region of
The first detection unit is arranged at a position different from the first detection unit, is irradiated from the first irradiation unit, and is irradiated with blood flowing through the flow channel. A third detection unit that detects light scattered in the area on the side where the 1 irradiation unit is arranged;
Total protein contained in blood using data indicating the relationship between the detection results of the first detection unit , the second detection unit , and the third detection unit , and the amount of total protein contained in the blood and the detection results a specific part that specifies the amount of
total protein amount measuring device. irradiating a flow channel for flowing blood with light from a first irradiation unit in a direction oblique to the blood flow direction of the flow channel;
By the blood irradiated from the first irradiation unit and flowing through the flow channel, the region on the side where the first irradiation unit is arranged among the regions facing each other through the flow channel in the radial direction of the flow channel. detecting the scattered light by the first detection unit;
By the blood irradiated from the first irradiation unit and flowing through the flow channel, the area on the side where the first irradiation unit is arranged among the regions facing each other via the flow channel in the radial direction of the flow channel. Detecting the light scattered to the area on the opposite side by the second detection unit,
By the blood irradiated from the first irradiation unit and flowing through the flow channel, the region on the side where the first irradiation unit is arranged among the regions facing each other through the flow channel in the radial direction of the flow channel. Scattered light is detected by a third detection unit arranged at a position different from the first detection unit,
detection results of the first detection unit , the second detection unit , and the third detection unit , and data indicating the relationship between the detection results and the amount of total protein contained in blood specifying an amount by a specified part,
Total protein amount measurement method.

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