JP7257715B2 - oral composition - Google Patents

Description

The present invention relates to oral compositions containing charcoal and certain other ingredients.

Most of charcoal is a porous substance composed of oxygen, hydrogen, calcium, etc. in addition to carbon, and has the property of adsorbing various substances in its fine pores.

Charcoal is industrially used as a decolorizing agent by adsorbing coloring components (Patent Document 1) and a deodorizing agent by adsorbing odorous substances (Patent Document 2), and is also used as an aid for filtration and purification. . Also, in recent years, attention has been focused on supplements that utilize the effects of charcoal.

JP-A-6-65591 JP 2013-81649 A

However, when charcoal is used in oral compositions such as supplements, the effects of charcoal have not been sufficiently investigated.

An object of the present invention is to provide a highly versatile oral composition for the purpose of effective utilization of charcoal.

As a result of intensive investigation and research on the functions of charcoal, the present inventors have found that by combining charcoal and 3-hydroxy-3-methylbutyric acid, creatine, amino acids or peptides (hereinafter also referred to as specific other materials), We have found that charcoal improves the undesirable taste such as bitterness of specific other materials, improves hardness when blended in tablets, and exerts excellent muscle strengthening action, leading to the present invention. rice field.

That is, the present invention is as follows.
[1] An oral composition comprising charcoal and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides.
[2] A muscle building oral composition comprising charcoal and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides.
[3] An oral composition comprising charcoal and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides, The oral composition having improved taste.
[4] A tablet characterized by containing charcoal and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides.

According to the present invention, by combining charcoal with other specific materials, it is possible to provide an oral composition in which charcoal improves the taste of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides. Moreover, according to the present invention, tablets with improved hardness can be provided. Furthermore, according to the present invention, it is possible to provide an oral composition that can be expected to have a remarkable muscle-enhancing effect.

The myoblast activating action when Examples 8 to 11 and Comparative Examples 6 to 10 are applied is shown. The myotube cell activating action when Examples 12, 14 to 16 and Comparative Examples 11, 12, 14 to 16 are applied is shown. The myotube cell activating action when Examples 17 to 21 and Comparative Examples 11 to 16 are applied is shown.

The composition of the present invention is characterized by containing charcoal and at least one other material selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides. The specific other materials used together with charcoal may be used singly or in combination of two or more.

ADVANTAGE OF THE INVENTION According to this invention, the combination of charcoal and a specific other material can provide the composition with improved taste derived from a specific other material. Therefore, the composition of the present invention has high utility as an oral composition.

According to the present invention, the combination of charcoal and other specific materials improves tablet hardness when formed into tablets. Therefore, the composition of the present invention has high utility as a tablet.

According to the present invention, a combination of charcoal and other specific materials exhibits a dramatic muscle building effect. Therefore, the composition of the present invention has high utility as an oral muscle-building composition.

Materials used in the present invention are described in detail below.

[Charcoal]
Charcoal is made by treating plants, compounds, and synthetic resins under high temperature conditions, and is mainly used as a fuel and as an aid for filtration and purification. The charcoal raw material that can be used in the present invention is not particularly limited as long as it can be taken orally. Husks, rice husks, and the like can be mentioned, and bamboo, Japanese red pine, coconut husks, and rice husks are preferred, and bamboo is particularly preferred. In addition, as the charcoal, it is preferable to use activated charcoal obtained by dry-distilling the raw material and activating it by high temperature treatment. It is most preferred to use activated carbon derived from bamboo, as it exerts an action as well as a muscle strengthening action. The activated carbon is not particularly limited as long as the adsorption capacity is activated. For example, in the case of a commercially available product, there is a description of activated carbon in the specifications of raw materials. The shape of the charcoal in the present invention is not limited as long as it is suitable for food, and examples thereof include powder, granules, fibrous forms, and formed charcoal, with powder and granules being preferred.

[Specific other materials]
The composition of the present invention contains a specific other material (at least one selected from 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides) as an essential component. Specific other materials are described in detail below.

(3-hydroxy-3-methylbutyric acid)
3-Hydroxy-3-methylbutyric acid (HMB) is a metabolite of the essential amino acid leucine and is synthesized in the human body. HMB in the present invention may be any physiologically available form of HMB, such as the free acid and salts, and derivatives thereof. The form of HMB is not particularly limited, but includes salts such as free acid, calcium salt, sodium salt, potassium salt and magnesium salt, esters, lactones, hydrates and the like. Calcium salts (HMBCa) are particularly preferred because they exhibit excellent tablet hardness-improving and muscle-enhancing effects. HMB in the oral composition of the present invention can be synthesized by a known method or a commercially available product. Commercial products are not limited as long as they are applicable to foods.

(creatine)
Creatine is one of organic acids biosynthesized from arginine, glycine and methionine in the body of animals. Creatine reacts with ATP and becomes an energy storage form, creatine phosphate, by possessing an energy-rich phosphate group. Creatine phosphate synthesizes ATP and supplies energy by supplying phosphate groups when energy is insufficient. Any physiologically available form of creatine, such as the free acid and salts, and derivatives thereof, may be used in the present invention. The form of creatine is not particularly limited, but includes salts such as free acid, calcium salt, sodium salt, potassium salt and magnesium salt, esters, lactones, hydrates, etc. Among them, creatine calcium salt and creatine monohydrate. and tricreatine malate are preferred, and creatine monohydrate is particularly preferred because it exhibits excellent tablet hardness improving action and muscle building action when combined with charcoal. Creatine in the oral composition of the present invention can be synthesized by a known method or a commercially available product. Commercial products are not limited as long as they are applicable to foods.

(amino acid)
Amino acids are organic compounds having both amino and carboxyl functional groups, amino acids contained as residues in proteins of animals such as humans, and naturally occurring amino acids not contained in proteins and their salts. is mentioned. In the present invention, for example, alanine, arginine, asparagine, aspartic acid, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, leucine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, cysteine, valine, cystine, hydroxyproline , citrulline, ornithine, etc., and arginine, glutamine, isoleucine, leucine, citrulline, valine, ornithine are preferred. Leucine and isoleucine are particularly preferred. Two or more amino acids may be used in combination. In the present invention, amino acids may be obtained from natural materials containing amino acids by extraction or the like, or industrially produced amino acids may be used. Moreover, in this invention, a commercial item can also be used.

(peptide)
A peptide refers to a substance and salts thereof in which two or more amino acids are dehydrated between amino and carboxyl groups and combined to form a peptide bond. That is, peptides in the present invention include, for example, oligopeptides with 10 or less amino acids, polypeptides and proteins with 11 or more amino acids, and the like. Peptides include, for example, imidazole dipeptides carnosine, anserine and valenine, as well as kyotorphin, aspartame, gloline, valetin, pseudoproline, glycylglutamine, glycyltyrosine, alanylglutamine, glycylglycine, homoanserine, and glutathione. , ophthalmic acid, nor-ophthalmic acid, isoleucine-proline-proline (IPP), glypromate, leupeptin, eisenin, collagen, peptide hormones (melanostatin, thyrotropin-releasing hormone, growth hormone, insulin, glucagon, etc.), cattle, Examples include animal proteins derived from livestock meats such as pigs and chickens, fish, milk and eggs and their hydrolyzed peptides, and vegetable proteins derived from soybeans, wheat, corn and peas and their hydrolyzed peptides. In the present invention, the peptide is preferably carnosine, anserine, balenine, collagen peptide, milk protein, soybean protein, or collagen. , anserine, valenin, and milk proteins are particularly preferred. Peptides may be used in combination of two or more. In addition, industrially produced peptides may be used as the peptides of the present invention. Moreover, in this invention, a commercial item can be used.

According to the present invention, the combination of charcoal and a specific other material improves the taste of the other material with the charcoal. Therefore, by using the composition of the present invention, the use of charcoal can be expanded because the effect of improving taste by charcoal is expected.

Further, according to the present invention, the combination of charcoal and other specific materials reduces the hardness of tablets. Therefore, by adopting the composition of the present invention, it is expected that the hardness of the tablet will be reduced, thereby improving the disintegration in the gastrointestinal tract.

Moreover, the composition of the present invention exerts a dramatic muscle strengthening effect by blending charcoal with other specific materials. For example, pharmaceuticals (including quasi-drugs), so-called health foods such as food for specified health use, food with nutrient function claims, functional food whose efficacy has been approved by a designated organization, and general It can be used as a food, a food additive, and the like. Therefore, since the composition of the present invention has high utility as an oral composition for muscle building, the use of charcoal can be expanded.

The composition of the present invention can be used as an oral muscle-building composition for muscle building. In that respect, the product is not particularly limited as long as it can be distinguished from other products. The scope of the present invention includes those indicating that there is an action function. The muscle-building oral composition of the present invention is not limited to one in which the components of the combination (charcoal and other specific materials) of the present invention are indicated as active ingredients for muscle-building on the packaging of the product. For example, the active ingredient may not be specified, or only charcoal or other specified material may be indicated as the active ingredient.

Specifically, the muscle-building oral composition of the present invention includes pharmaceuticals (including quasi-drugs) and so-called health foods. Suppresses muscle breakdown, Strengthens muscle strength, Suppresses/maintains/improves/improves walking ability, Improves metabolism by building muscle, Suppresses metabolic decline, Reduces muscle fatigue Prevention and recovery", "prevention and maintenance of loss of balance ability", "shape up", "macho", "thin macho", "beautiful body", "bedridden prevention", "bedridden prevention", "fall prevention", " Examples include those indicated as "Aiming to improve metabolism by building muscle" and "Maintaining muscle mass and strength."

The content of charcoal and other specific materials (hereinafter sometimes referred to as components of the present invention) in the composition of the present invention may be appropriately contained within the range in which the effects thereof are exhibited.

Specifically, when the oral composition of the present invention is a tablet, round, capsule-shaped supplement or drug, the component of the present invention should be contained in an amount of 5 to 99% by mass in terms of dry mass. is preferred, more preferably 8 to 98% by mass, and particularly preferably 10 to 97% by mass. In addition, when the oral composition of the present invention is a chewable supplement or pharmaceutical, the component of the present invention preferably contains 0.1 to 70% by mass of the total in terms of dry mass, and 0.5 to 70% by mass. It is more preferably contained in an amount of 60% by mass, and particularly preferably contained in an amount of 1 to 50% by mass. In the case of powdery or granular supplements and pharmaceuticals, the component of the present invention preferably contains 0.1 to 90% by mass of the total in terms of dry mass, and 0.5 to 80% by mass. is more preferable, and it is particularly preferable that it is contained in an amount of 1 to 70% by mass. In the case of liquid supplements and pharmaceuticals, the component of the present invention preferably contains 5 to 99% by weight of the total dry weight, more preferably 8 to 98% by weight, and 10 to 97% by weight. % is particularly preferred.

There is no particular limit to the amount of intake of the charcoal of the present invention. The daily intake of charcoal is preferably 1 mg/day or more, more preferably 5 mg/day or more, and preferably 10 mg/day or more. More preferred. The upper limit is, for example, 50000 mg/day, preferably 30000 mg/day, more preferably 10000 mg/day.

The intake of HMB of the present invention is not particularly limited, but from the viewpoint of more remarkably exerting the tablet hardness improving action and muscle strengthening action when combined with other materials, the daily dose for adults is 100 mg/day. It is preferable to ingest at 200 mg/day or more, and it is further preferable to ingest at 300 mg/day or more. The upper limit is, for example, 10000 mg/day, preferably 8000 mg/day, more preferably 5000 mg/day. The amount of intake of creatine, amino acids, and peptides of the present invention is not particularly limited, but is preferably 0.001 mg/day or more, preferably 0.005 mg/day or more, per day for adults. more preferably 0.01 mg/day or more. The upper limit is, for example, 10000 mg/day, preferably 5000 mg/day, more preferably 1000 mg/day. The oral composition of the present invention may be appropriately designed so that the daily intake is the above-described intake amount, and may be taken in one dose or divided into multiple doses. For example, in the case of tablets, capsules, rounds, or chewables, the number of intakes is 1 to 9 times per day, and the total intake should be the above amount. The oral composition of the present invention can be contained in one container or divided into a plurality of containers, for example 2 to 3, so that the daily intake is equal to the above-mentioned intake for one day.

The mixing mass ratio of charcoal and HMB is preferably in the range of 1:0.01 to 5000, more preferably in the range of 1:0.1 to 3000, more preferably in the range of 1:1 to 1:1 in terms of dry mass. A range of 1000 is more preferable, and a range of 1:3 to 300 is particularly preferable from the viewpoint of more remarkably exhibiting an excellent tablet hardness-improving effect and muscle-building effect when combined. The blending mass ratio of charcoal and creatine, amino acid or peptide is preferably in the range of 1:0.000001 to 30, more preferably in the range of 1:0.000003 to 10 in terms of dry mass, It is more preferably in the range of 1:0.000005 to 1, and from the viewpoint of more remarkably exhibiting the excellent tablet hardness improving action and muscle strengthening action when combined, 1:0.00001 to 0.1 A range is particularly preferred.

With respect to the total amount of the ingredients of the present invention, in the case of tablets, rounds, capsules, chewables, powders, granules supplements and pharmaceuticals, charcoal is 0.0001 to 70% by mass in terms of dry mass. It is preferably contained, more preferably 0.001 to 50% by mass, from the viewpoint of more significantly exhibiting the excellent tablet hardness improving action and muscle strengthening action when combined with other materials. , is particularly preferably contained in an amount of 0.01 to 30% by mass. In the case of liquid supplements and pharmaceuticals, it is preferable that charcoal is contained in an amount of 0.000001 to 10% by mass, more preferably 0.00001 to 5% by mass, in terms of dry mass. From the viewpoint of more remarkably exhibiting the excellent tablet hardness-improving action and muscle-building action when combined, it is particularly preferred that they are contained in an amount of 0.0001 to 3% by mass.

Examples of the form of the composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, and caplets. , gel-like, chewable, stick-like and the like. Among these, tablets, capsules, powders, granules, and liquid are preferred, and tablets and granules are particularly preferred. Specific examples include supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant beverages dissolved in water (hot water), milk, fruit juice, etc., and food additives. be able to. In particular, supplements and instant beverages are preferable because they are easy to drink and can enhance palatability.

When the composition of the present invention is in the form of tablets, rounds, or chewables, by adding one or more of excipients, lubricants, and fluidizing agents, moldability is enhanced and the obtained tablets are , round, and chewable compositions to improve storage stability. In particular, storage stability can be further enhanced by using excipients and lubricants. Excipients are added to improve the handling or molding of the composition, or to facilitate administration. Excipients that can be used in the present invention are not particularly limited. Yeast, low-substituted hydroxypropyl cellulose, hydroxypropyl cellulose, refined sucrose, light anhydrous silicic acid, calcium silicate, titanium oxide, precipitated calcium carbonate, reduced maltose, maltose and the like. These may be used individually by 1 type, and may use 2 or more types together. In the present invention, hydroxypropyl cellulose, powdered cellulose, pregelatinized starch, pregelatinized starch, sugar alcohol, reduced maltose, and maltose are preferably used as excipients from the viewpoint of moldability and storage stability. Lubricants are used to reduce friction between the punches of a tableting machine and tablets when compressing powder for tablets, and to prevent tableting failures such as sticking. The lubricant that can be used in the present invention is not particularly limited as long as it is a component that can achieve the above purpose. Stearyl sodium, sucrose fatty acid ester, talc, polyethylene glycol, vegetable oil, hardened oil and the like. These may be used individually by 1 type, and may use 2 or more types together. In the present invention, calcium stearate and sucrose fatty acid ester are preferably used as the lubricant from the viewpoint of moldability and storage stability. A fluidizer is used to improve the fluidity of mixed powder and granules. The fluidizing agent that can be used in the present invention is not particularly limited, and examples thereof include silicon dioxide, aluminum silicate, magnesium aluminosilicate, calcium phosphate, magnesium carbonate, and magnesium oxide. These may be used individually by 1 type, and may use 2 or more types together. In the present invention, silicon dioxide and magnesium carbonate are preferably used as the fluidizing agent from the viewpoint of moldability and storage stability. In the present invention, commercial products can be used as excipients, lubricants, and fluidizers.

The composition of the present invention can be produced by a known method by adding other components other than the components of the present invention, if necessary. Other ingredients other than the ingredients of the present invention include, for example, water-soluble vitamins (vitamin B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P), oil-soluble vitamins (vitamins A, D, E, K); minerals such as calcium, magnesium, phosphorus, and iron; sulfur-containing compounds contained in taurine, garlic, etc.; hesperidin, quercetin, etc. Flavanoids or flavonoids; animal oils and fats; vegetable oils and fats; pulverized products or extracts of animals and plants, and the like.

The present invention will be described below based on examples, but the present invention is not limited to these examples, and the present invention can take various aspects as long as the problems of the present invention can be solved.

The following substances were used as test substances in Tests 1 and 2.
・As charcoal, bamboo-derived activated carbon powder (commercially available) was used.
- HMBCa (commercially available) was used as HMB or a derivative thereof.
- Creatine monohydrate (commercially available) was used as creatine.
- L-isoleucine (commercially available) was used as an amino acid.
- Collagen peptide (commercially available) was used as the peptide.
- Soybean protein (commercially available) was used as the protein.

[Test 1: Sensory evaluation of oral composition]
(1) Preparation of samples For the samples of Comparative Examples 1 to 5 and Examples 1 to 7 shown in Table 1 below, after mixing each raw material in the described compounding ratio, using a tableting machine, 300 mg per tablet , tablets with a tablet diameter of 9 mm were produced. The unit of numerical values in the table is %.

(2) Sample Evaluation Five healthy adults were randomly selected as subjects. A questionnaire was conducted on the evaluation items shown in Table 2 below for these five subjects, and a sensory evaluation was performed. Specifically, the samples of each example were evaluated with any of the comparative examples as a reference (0 points), and scores ranging from -4 to +4 were given. As for the evaluation, −4 point is the worst and +4 point is the best. Table 3 below shows the average scores of five subjects for each evaluation item. "Average" in Table 3 is the average score of each evaluation item. Table 3 below shows comparative examples that serve as a reference for each example.

(3) Consideration Evaluation results of palatability were considered for each of the comparative examples and examples.

As shown in Table 3, Comparative Examples 1 to 5, in which HMBCa, creatine, isoleucine, collagen peptide, or soybean protein was blended, and charcoal were combined, compared to Comparative Examples 1 to 5, bitterness, harshness, Improved taste, mildness, odor and sweetness. Also, Examples 6 and 7, in which charcoal was added to the combination of HMBCa and soy protein and the combination of isoleucine and creatine, were also improved in bitterness, harshness, mildness, odor, and sweetness compared to Comparative Examples 2 and 3. Therefore, it was found that when charcoal is combined with other materials of the present invention, a composition in which the charcoal improves the taste of the other materials can be obtained.

[Test 2: Tablet hardness evaluation]
(1) Sample Evaluation Examples 1 to 5 and Comparative Examples 1 to 5 prepared in Test 1 were evaluated using a tablet hardness tester (manufactured by Okada Seiko Co., Ltd.). Table 4 shows the results.

(2) Consideration Evaluation results of tablet hardness were considered for each of Comparative Examples and Examples.

It is generally known that the lower the tablet hardness, the better the disintegration in the gastrointestinal tract. As shown in Table 4, Examples 1 to 5, in which HMBCa, creatine, isoleucine, collagen peptide, or soybean protein and charcoal are combined, have lower tablet hardness than Comparative Examples 1 to 5, in which only other materials are blended. bottom. Therefore, by combining charcoal with other specific materials, it is possible to reduce the hardness of the tablet, so that it is expected that a tablet that disintegrates well in the gastrointestinal tract and has an excellent immediate effect can be obtained.

As test substances in Tests 3 and 4, the following were used.
・As charcoal, bamboo-derived activated carbon powder (commercially available) was used.
- HMBCa (commercially available) was used as HMB or a derivative thereof.
- Creatine monohydrate (commercially available) was used as creatine.
- L-leucine (commercially available) was used as an amino acid.
- As a peptide, imidazole dipeptide (chicken extract containing 15% or more of anserine and carnosine; commercially available product) was used.
- Milk protein (commercially available) was used as the protein.

[Test 3: Evaluation of myoblast activation effect]
(1) A mouse myoblast cell line (C2C12) was cultured in DMEM containing 10% (V/V) FBS in a 75 cm2 flask at 37°C in a 5% CO2 incubator.
(2) Cells suspended by trypsin treatment were seeded at a cell density of 10,000 cells/well in each well of a collagen-coated 96-well plate from a 75 cm 2 flask.
(3) Precultured for 24 hours in a 37°C, 5% CO2 incubator.
(4) 0.5% (V/V) DMSO-10% (V/V ) 200 μL of DMEM containing FBS was added and cultured in a 37° C., 5% CO 2 incubator for 20 hours. As a control, DMEM containing 0.5% (V/V) DMSO-10% (V/V) FBS containing no test substance adjusted to 7.5 μM H2O2 was used. Concentration units in Table 5 are μg/mL.
(5) After removing the medium, each well was washed once with 200 μL/well of PBS. Then, 150 μL/well of Cell Counting Kit-8 (manufactured by Dojindo Laboratories) solution diluted 30-fold with DMEM was added.
(6) The plate after the addition was allowed to stand still in a 37° C., 5% CO2 incubator to allow appropriate color development, then 100 μL was dispensed from each well into a new 96-well plate, and the absorbance of each well at 450 nm was measured. bottom. Based on the obtained data, the cell survival rate (% of control) was calculated based on the following formula (1).

式（１）中、「Ｄａｔａ ｓａｍｐｌｅ」は各被験物質添加ウェルの吸光度を表し、「Ｄａｔａ ｃｏｎｔｒｏｌ」はコントロールのウェルの吸光度を表し、「Ｄａｔａ ｂｌａｎｋ」は細胞がないウェルの吸光度を表す。

In formula (1), "Data sample" represents the absorbance of each test substance-added well, "Data control" represents the absorbance of control wells, and "Data blank" represents the absorbance of wells without cells.

FIG. 1 shows the measurement results of the myoblast activating action. A larger value indicates a higher myoblast activation activity.

As shown in FIG. 1, under conditions in which myoblasts were subjected to cytotoxicity by H2O2, a composition combining charcoal and HMBCa, imidazole dipeptide, milk protein or creatine was more effective than each material alone. , showed an excellent myoblast activation effect. Therefore, it can be seen that the composition of the present invention has an excellent muscle-enhancing effect.

[Test 4: Evaluation of myotube activation effect]
(1) A mouse myoblast cell line (C2C12) was cultured in DMEM containing 10% (V/V) FBS in a 75 cm2 flask at 37°C in a 5% CO2 incubator.
(2) Cells suspended by trypsin treatment were seeded from a 75 cm 2 flask into each well of a collagen-coated 96-well plate at a cell density of 5000 cells/well.
(3) Precultured for 48 hours in a 37°C, 5% CO2 incubator.
(4) The medium was prepared from DMEM containing 10% (V/V) FBS to differentiate the medium (100 ng/mL human insulin solution and 2% (V/V) horse serum 10% (V/V ) FBS-containing DMEM) and cultured in a 37° C., 5% CO ₂ incubator to induce differentiation into myotube cells.
(5) Add 200 μL each of 0.5% (V/V) DMSO-containing differentiation induction medium containing the test substance at a predetermined concentration shown in Table 6 below, prepared so that H2O2 becomes 30 μM, and heat at 37° C. for 5 It was cultured for 20 hours in a % CO2 incubator. As a control, a differentiation-inducing medium containing 0.5% (V/V) DMSO containing no test substance adjusted to 30 μM H2O2 was used. Concentration units in Table 6 are μg/mL.
(6) After removing the medium, each well was washed once with 200 μL/well of PBS. Then, 150 μL/well of Cell Counting Kit-8 (manufactured by Dojindo Laboratories) solution diluted 30-fold with DMEM was added.
(7) After the addition, the plate was allowed to stand still in a 37°C, 5% CO2 incubator for appropriate color development, then 100 µL was dispensed from each well into a new 96-well plate, and the absorbance of each well at 450 nm was measured. bottom. Based on the obtained data, the cell activation activity (% of control) relative to the control was calculated based on the following formula (1).

式（１）中、「Ｄａｔａ ｓａｍｐｌｅ」は各被験物質添加ウェルの吸光度を表し、「Ｄａｔａ ｃｏｎｔｒｏｌ」はコントロールのウェルの吸光度を表し、「Ｄａｔａ ｂｌａｎｋ」は細胞がないウェルの吸光度を表す。

In formula (1), "Data sample" represents the absorbance of each test substance-added well, "Data control" represents the absorbance of control wells, and "Data blank" represents the absorbance of wells without cells.

Figures 2 and 3 show the measurement results of myotube activation. A larger value indicates a higher myotube activation activity.

As shown in FIGS. 2 and 3, a composition combining charcoal and one or more other materials selected from HMBCa, leucine, milk protein, and creatine under conditions in which myotube cells were subjected to cytotoxicity by H2O2 , showed superior myotube cell activating action compared to each material alone. In addition, Example 13, in which charcoal and imidazole dipeptide were combined, also exhibited superior myotube cell activating action compared to Comparative Examples 11 and 13, in which each was used alone. Therefore, it can be seen that the composition of the present invention has an excellent muscle-enhancing effect.

[Production Example 1] (Production of tablets)
Tablets (450 mg/tablet) containing the following ingredients were formed using a tableting machine. When the taste of the obtained tablets was evaluated, it was found that the unfavorable taste of other ingredients had been improved. In addition, when 9 tablets of the obtained tablets were ingested a day, it was confirmed that they had a muscle-enhancing effect.

Charcoal powder (activated bamboo charcoal) 3%
HMBCa 75%
Creatine Monohydrate 0.01%
L-valine 0.05%
L-isoleucine 0.05%
L-Leucine 0.1%
Chicken extract 0.001%
(Contains 15% or more of anserine and carnosine)
L-glutamine 0.01%
L-ornithine hydrochloride 0.01%
L-arginine 0.01%
L-citrulline 0.01%
Milk protein 0.001%
Hydroxypropyl cellulose 4%
Crystalline cellulose 10%
Calcium stearate 2%
Silicon dioxide 1%
Reduced maltose Remainder

[Production Example 2] (Production of tablets)
Tablets (250 mg) containing the following ingredients were formed using a tableting machine. When the taste of the obtained tablets was evaluated, it was found that the unfavorable taste of other ingredients had been improved. In addition, when the obtained tablets were ingested five times a day, it was confirmed that they had a muscle-enhancing effect.

Charcoal powder (activated carbon of rice husk) 10%
HMBCa 50%
Creatine Monohydrate 1%
Collagen peptide 0.01%
Ground black garlic powder 3%
Lactic acid bacteria 0.1%
Calcium stearate 2%
Silicon dioxide 2%
Crystalline cellulose 10%
Reduced maltose Remainder

[Production Example 3] (Production of granules)
After mixing the following ingredients, granules were produced by applying a continuous fluid granulation drying apparatus. When the taste of the obtained granules was evaluated, it was found that the unfavorable taste of other materials was improved. In addition, when the obtained granules were ingested, it was confirmed that they had a muscle-enhancing effect.

Charcoal powder (activated bamboo charcoal) 5%
HMBCa 50%
Chicken extract 1%
(Contains 15% or more of anserine and carnosine)
Glucosylceramide derived from rice 2%
Aspartame 0.1%
Thiamine hydrochloride 0.5%
Riboflavin 0.5%
Vitamin B6 0.2%
Cyanocobalamin 0.2%
Perfume 0.2%
Reduced palatinose 7%
Sucrose fatty acid ester 5%
hydroxypropyl cellulose balance

[Production Example 4] (Production of instant powdered beverage)
After mixing the following ingredients, they were subjected to a continuous fluid granulation drying apparatus to produce a powder for use in instant powdered beverages. When the obtained powder was dissolved in 100 mL of water to evaluate the taste of an instant powdered drink, it was found that the unfavorable taste of other materials was improved. In addition, when the obtained granules were ingested, it was confirmed that they had a muscle-enhancing effect. In addition, the ratio of a component is a value of dry mass conversion.

Charcoal powder (activated charcoal from coconut shells) 3%
Charcoal powder (activated charcoal from red pine) 1%
Charcoal powder (activated carbon of rice husk) 1%
HMBCa 3%
1% soy protein
Chia seed ground powder 3%
Barley grass extract powder 1%
Aspartame 1%
Erythritol 0.1%
Sucralose 5%
Malic acid 0.5%
Vitamin mix 1%
Pigment formulation 0.5%
Perfume 0.3%
polydextrose balance

According to the present invention, by combining charcoal and at least one selected from the group consisting of 3-hydroxy-3-methylbutyric acid, creatine, amino acids and peptides, charcoal improves taste and reduces tablet hardness. , and can expand the use of charcoal in food and beverages. Furthermore, as an effect of the present invention, it is possible to provide an oral composition having an added value of muscle-enhancing action.

Claims (2)

A tablet comprising charcoal and one or more amino acids selected from arginine, glutamine, isoleucine, leucine, citrulline, valine and ornithine (excluding those derived from brewer's yeast). A tablet comprising charcoal powder and one or more peptides selected from collagen peptides, milk proteins and soybean proteins.

Images