JP7239794B1 - Dairy cow feeding method - Google Patents

Dairy cow feeding method Download PDF

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JP7239794B1
JP7239794B1 JP2022032291A JP2022032291A JP7239794B1 JP 7239794 B1 JP7239794 B1 JP 7239794B1 JP 2022032291 A JP2022032291 A JP 2022032291A JP 2022032291 A JP2022032291 A JP 2022032291A JP 7239794 B1 JP7239794 B1 JP 7239794B1
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隆晴 門坂
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Abstract

【課題】 分娩後においても、乳牛の尿pH値の上昇を抑制する。【解決手段】 乾乳期間中に、サイレージや繊維質飼料などの粗飼料と、配合飼料とを混合して調整した混合飼料を給与すること、タンパク質分解酵素を含むパイナップル由来飼料を給与すること、前記タンパク質分解酵素は、酵素活性度が1日あたり600GDU以上となるように給与することを特徴とする乳牛の飼料給与方法。また、パイナップル粕を主原料として、カシューナッツ殻、酵素処理パイナップル根茎副産物を混合したパイナップル由来飼料を、前記混合飼料内に均一になるように混ぜて給与する。【選択図】 図4An object of the present invention is to suppress an increase in the urine pH value of dairy cows even after parturition. [Solution] Feeding a mixed feed prepared by mixing rough feed such as silage or fibrous feed with compound feed during a dry milk period, feeding a pineapple-derived feed containing a proteolytic enzyme, A feed feeding method for dairy cattle, wherein the protease is fed so that the enzyme activity is 600 GDU or more per day. Also, a pineapple-derived feed prepared by mixing cashew nut shells and enzyme-treated pineapple rhizome by-products with pineapple meal as the main raw material is uniformly mixed in the mixed feed and fed. [Selection drawing] Fig. 4

Description

本発明は、乳牛の飼料給与方法及び飼料に関する。 The present invention relates to a feed feeding method and feed for dairy cows.

従来、乳牛の飼料給与方法はいろいろ提案されている。例えば特許文献1では、分娩直前から分娩後30日以内に発症しやすい乳牛の乳熱(起立不能)や低カルシウム血症を予防するための、飼養管理の方法を提案している。 Conventionally, various methods of feeding feed to dairy cows have been proposed. For example, Patent Literature 1 proposes a feeding management method for preventing milk fever (impossibility to stand) and hypocalcemia in dairy cows, which tend to develop from just before parturition to within 30 days after parturition.

一般に、イオンバランスに注意した飼料給与を行うが、イオンバランスが適切であるかどうかは、尿pH値を測り、確認される。尿pH値が、体内のイオンバランス(血液pH)の目安となるからである。尿pH値が8台で高産次の牛であれば、分娩後低カルシウム血症になる可能性があり、尿pH値を低くしたいという要求がある。 In general, feed is given while paying attention to the ion balance, and whether the ion balance is appropriate or not is confirmed by measuring the urine pH value. This is because the urine pH value serves as a measure of the ion balance (blood pH) in the body. A cow with a urine pH value of 8 and a high calf may develop postpartum hypocalcemia, and there is a demand to lower the urine pH value.

一方、本願の発明者は、以前から牛乳や乳牛の飼育方法、飼料などの研究を行っており、例えば睡眠を誘導する牛乳などの機能性を有する牛乳や乳牛用の飼料を開発している(特許文献2、3参照)。今般、上記の課題についても、これを解決すべく、乳牛用の飼料の研究を進めていた。 On the other hand, the inventor of the present application has been researching methods of raising milk and dairy cows, feed, etc., and has developed functional milk and feed for dairy cows, such as milk that induces sleep ( See Patent Documents 2 and 3). In order to solve the above problems, we have been researching feed for dairy cows.

特開2009-022258号公報JP 2009-022258 A 特開2016-082961号公報JP 2016-082961 A 特開2017-169451号公報JP 2017-169451 A

そして、本願の発明者は、タンパク質分解酵素を含む飼料が、尿pH値に影響があることに注目し研究を重ねたところ、タンパク質分解酵素を含むパイナップル由来飼料を、乾乳期間中の一定期間において、一定の方法で乳牛に給与することで、分娩後において尿pH値の上昇抑制に大きく貢献することを見いだし、本発明をなしたものである。 The inventors of the present application focused on the fact that the feed containing protease has an effect on the urine pH value, and after repeated research, the pineapple-derived feed containing protease was added for a certain period of time during the dry period. In the above, the present inventors have found that when fed to dairy cows in a certain manner, it greatly contributes to suppressing the increase in urine pH value after parturition, and have made the present invention.

本発明は、分娩後においても、尿pH値の上昇が抑制される乳牛の飼料給与方法並びに乳牛用飼料を提供する。 INDUSTRIAL APPLICABILITY The present invention provides a feed feeding method for dairy cows and a feed for dairy cows, in which an increase in urine pH value is suppressed even after parturition.

本発明の一の態様の乳牛の飼料給与方法は、乾乳期間中に、サイレージや繊維質飼料などの粗飼料と、配合飼料とを混合して調整した混合飼料を給与すること、乾乳期間中に、タンパク質分解酵素を含むパイナップル由来飼料を給与すること、前記タンパク質分解酵素は、酵素活性度が1日あたり600GDU以上となるように給与することで、分娩後における尿pHの上昇が抑制されることを特徴とする。
A feed feeding method for dairy cattle according to one aspect of the present invention comprises feeding mixed feed prepared by mixing rough feed such as silage and fiber feed with compound feed during the dry period, and feeding during the dry period. In addition, feeding a pineapple-derived feed containing a protease, and feeding the protease so that the enzyme activity is 600 GDU or more per day, suppresses the increase in urine pH after delivery. It is characterized by

ここで、GDUはゼラチン消化単位である。このようにすれば、乾乳期間中に、タンパク質分解酵素の酵素活性度が所定分量以上含むようにパイナップル由来飼料を給与することで、分娩後においても、尿pH値上昇を抑制することができる。なお、配合飼料には、例えばビタミンなどの栄養が調整された乾乳期用の配合飼料などを用いることができる。 where GDU is gelatin digestion unit. In this way, by feeding the pineapple-derived feed so that the enzymatic activity of the protease is contained in a predetermined amount or more during the dry milk period, it is possible to suppress an increase in the urine pH value even after parturition. . As the mixed feed, for example, a mixed feed for the dry period in which nutrients such as vitamins are adjusted can be used.

また、この乳牛の飼料給与方法において、前記タンパク質分解酵素は、酵素活性度が1日あたり600GDU~2400GDUとなるように給与することが望ましい。このようにすれば、尿pH値上昇をさらに抑制することができる。 Further, in this feed feeding method for dairy cows, the protease is desirably fed such that the enzyme activity is 600 to 2400 GDU per day. By doing so, it is possible to further suppress an increase in the urine pH value.

また、この乳牛の飼料給与方法において、前記タンパク質分解酵素は、酵素活性度が1日あたり2400GDU以上となるように給与することが望ましい。このようにすれば、尿pH値上昇をさらに抑制することができる。 Moreover, in this feed feeding method for dairy cows, it is desirable to feed the proteolytic enzyme so that the enzyme activity is 2400 GDU or more per day. By doing so, it is possible to further suppress an increase in the urine pH value.

また、この乳牛の飼料給与方法は、前記パイナップル由来飼料を、前記混合飼料に均一になるように混ぜて給与することを特徴とする。このようにすれば、混合飼料とパイナップル由来飼料が均一に混ざり合うことで、乳牛の喫食がよくなり、効果的である。 In addition, this feeding method for dairy cows is characterized in that the pineapple-derived feed is uniformly mixed with the mixed feed and fed. By doing so, the mixed feed and the pineapple-derived feed are evenly mixed, which is effective because the dairy cows eat well.

また、この乳牛の飼料給与方法において、前記混合飼料は、前記粗飼料がグラスサイレージ及びコーンサイレージが含まれる粗飼料であり、これを前記配合飼料と混合して調製した混合飼料であることが望ましい。 Further, in this feed feeding method for dairy cows, it is preferable that the mixed feed be a mixed feed prepared by mixing the roughage with the mixed feed, wherein the roughage contains grass silage and corn silage.

また、この乳牛の飼料給与方法は、前記パイナップル由来飼料は、パイナップル粕を主原料として、カシューナッツ殻、酵素処理パイナップル根茎副産物を混合したものであることが望ましい。 Further, in this feed feeding method for dairy cows, it is desirable that the pineapple-derived feed be a mixture of cashew nut shells and enzyme-treated pineapple rhizome by-products with pineapple meal as the main raw material.

また、この乳牛の飼料給与方法は、前記乾乳期間の後期に前記混合飼料に加えて、乾乳牛用配合飼料をトップドレスで給与することが望ましい。 In addition, in this feed feeding method for dairy cows, it is desirable to top-dress the dry cow mixed feed in addition to the mixed feed in the latter half of the dry milk period.

また、この乳牛の飼料給与方法は、前記乾乳期間の後期が、前記乾乳期間の前期よりも前記混合飼料の給与量を少なくすることが望ましい。このようにすれば、尿pH値上昇を抑制することに好適である。 Further, in this feed feeding method for dairy cows, it is desirable that the amount of the mixed feed fed is smaller in the latter half of the dry-milk period than in the early period of the dry-milk period. This is suitable for suppressing an increase in urine pH value.

また、この乳牛の飼料給与方法は、前記混合飼料を朝夕2回に分けて給与すること、水、塩、乾草を自由採食とすることが望ましい。 In addition, it is desirable that the feed feeding method for the dairy cows is to feed the mixed feed twice in the morning and evening, and to allow free feeding of water, salt and hay.

また、この乳牛の飼料給与方法は、スタンチョンが設置されたフリーパンで飼養し、飼料給与時にはスタンチョンをかけて他の牛の飼料の盗食をするのを防止する、ことが望ましい。 In addition, in this feed feeding method for dairy cows, it is desirable that the cows be fed in a free pan with a stanchion installed, and that the stanchion be put on the feed to prevent it from stealing the feed of other cows.

本発明の一の態様の乳牛用飼料は、乾乳期間中に乳牛に給与することで、分娩後における尿pHの上昇が抑制される飼料であり、サイレージや繊維質飼料などの粗飼料と、配合飼料とを混合して調製した混合飼料と、タンパク質分解酵素を含むパイナップル由来飼料とを含み、前記タンパク質分解酵素が、乳牛の1日あたりの飼料に、酵素活性度が600GDU以上となる分量が含まれていることを特徴とする。 A dairy cow feed according to one aspect of the present invention is a feed that suppresses an increase in urine pH after parturition by being fed to dairy cows during the dry period. A mixed feed prepared by mixing feed and a pineapple-derived feed containing a protease, and the protease is included in the daily feed for dairy cows in an amount such that the enzyme activity is 600 GDU or more. It is characterized by being

このようにすれば、乾乳期間中に給与する飼料に、タンパク質分解酵素の酵素活性度が所定分量以上含むようにパイナップル由来飼料を含めることで、分娩後においても、尿pH値上昇を抑制することができる。 In this way, the pineapple-derived feed is included in the feed to be fed during the dry milk period so that the enzymatic activity of the protease is greater than or equal to a predetermined amount, thereby suppressing an increase in the urine pH value even after parturition. be able to.

また、この乳牛用飼料は、前記タンパク質分解酵素が、乳牛の1日あたりの飼料に、酵素活性度が600GDU~2400GDUとなる分量が含まれていることが望ましい。 In addition, it is desirable that the feed for dairy cows contains the proteolytic enzyme in such an amount that the daily feed for dairy cows has an enzyme activity of 600 to 2400 GDU.

また、この乳牛用飼料は、前記タンパク質分解酵素が、乳牛の1日あたりの飼料に、酵素活性度が2400GDU以上となる分量が含まれていることが望ましい。 In addition, it is desirable that the feed for dairy cows contains the proteolytic enzyme in such an amount that the daily feed for dairy cows has an enzyme activity of 2400 GDU or more.

また、この乳牛用飼料は、前記混合飼料は、前記粗飼料がグラスサイレージ及びコーンサイレージが含まれる粗飼料であり、これを前記配合飼料と混合して調製した混合飼料であることが望ましい。 Also, in this feed for dairy cattle, the mixed feed is desirably a mixed feed prepared by mixing the roughage containing grass silage and corn silage with the mixed feed.

また、この乳牛用飼料は、前記パイナップル由来飼料が、パイナップル粕を主原料として、カシューナッツ殻、酵素処理パイナップル根茎副産物を混合したものであることが望ましい。 Moreover, in this feed for dairy cows, the pineapple-derived feed is preferably a mixture of pineapple meal as a main raw material with cashew nut shells and enzyme-treated pineapple rhizome by-products.

本発明によれば、分娩後においても、尿pH値上昇を抑制することができる。 According to the present invention, an increase in urine pH value can be suppressed even after childbirth.

本発明に係る方法で飼料を給与したことによる試験結果を示す図である。FIG. 4 is a diagram showing test results obtained by feeding feed according to the method of the present invention. 本発明に係る方法で飼料を給与したことによる試験結果を示す図である。FIG. 4 is a diagram showing test results obtained by feeding feed according to the method of the present invention. 本発明に係る方法で飼料を給与したことによる試験結果を示す図である。FIG. 4 is a diagram showing test results obtained by feeding feed according to the method of the present invention. 本発明に係る方法で飼料を給与したことによる試験結果を示す図である。FIG. 4 is a diagram showing test results obtained by feeding feed according to the method of the present invention.

<実施形態>
以下、本発明に係る実施の形態を説明する。
本発明の一実施形態に係る乳牛の飼料給与方法は、乾乳期間中に、所定の乳牛用飼料を給与するものである。
<Embodiment>
BEST MODE FOR CARRYING OUT THE INVENTION Embodiments according to the present invention will be described below.
A feed feeding method for dairy cows according to an embodiment of the present invention feeds predetermined feed for dairy cows during a dry milk period.

本実施形態の一例として、この乳牛用飼料は、グラスサイレージ及びコーンサイレージを含む粗飼料と、ビタミンなどの栄養が調整された乾乳期用の配合飼料とを混合して調製した混合飼料を給与し、タンパク質分解酵素を含むパイナップル由来飼料を、混合飼料内に均一になるように混ぜて給与し、タンパク質分解酵素は、酵素活性度が1日あたり600GDU~2400GDUとなるようにしたものである。なお、粗飼料や配合飼料は、その他の粗飼料や配合飼料を用いることもでき、GDUはゼラチン消化単位である。 As an example of the present embodiment, this feed for dairy cows is a mixed feed prepared by mixing roughage containing grass silage and corn silage with mixed feed for the dry period in which nutrients such as vitamins are adjusted. , a pineapple-derived feed containing a protease was uniformly mixed in a mixed feed, and the protease was adjusted to have an enzyme activity of 600 to 2400 GDU per day. Other roughages and mixed feeds can also be used as roughages and mixed feeds, and GDU is a gelatin digestion unit.

また、この乳牛用飼料に含まれるパイナップル由来飼料は、パイナップル粕を主原料として、カシューナッツ殻、酵素処理パイナップル根茎副産物を混合したものである。 The pineapple-derived feed contained in this feed for dairy cows is a mixture of cashew nut shells and enzyme-treated pineapple rhizome by-products, with pineapple meal as the main raw material.

なお、乾乳期間とは、搾乳を始めて280日~300日たったら搾乳を止め、次の分娩に備えて所定期間乳牛を休ませる期間のことを言う。本実施形態においては、乾乳期間を分娩の8週前から分娩日までとし、乾乳前期を分娩予定日の8~3週前まで、乾乳後期を分娩予定日の3週前から分娩日までとして飼料を給与した。 The dry period refers to the period during which milking is stopped after 280 to 300 days from the start of milking and the cow is allowed to rest for a predetermined period in preparation for the next delivery. In this embodiment, the dry period is from 8 weeks before delivery to the delivery date, the early dry period is from 8 to 3 weeks before the expected delivery date, and the late dry period is from 3 weeks before the expected delivery date to the delivery date. Feed was fed until

この場合、乳牛用飼料を、1日朝夕2回に分けて給与するが、乾乳後期は、乾乳前期よりも混合飼料の給与量を少なくしている。具体的には、一例として、乾乳前期を16kg/日、乾乳後期を10kg/日給与した。 In this case, the feed for dairy cows is fed twice a day in the morning and evening, but the amount of mixed feed fed is smaller in the latter half of the dry period than in the early period of the dry period. Specifically, as an example, 16 kg/day was fed during the early dry period and 10 kg/day during the late dry period.

そして、乾乳後期には、混合飼料に加えて、乾乳牛用配合飼料をトップドレスで給与した。 In addition to the mixed feed, the mixed feed for dry cows was top-dressed in the late dry period.

また、水、塩、乾草は、自由採食とし、スタンチョンが設置されたフリーパンで飼養し、飼料給与時にはスタンチョンをかけて他の牛の飼料を、盗食をするのを防止する。 In addition, water, salt, and hay are fed freely, and the cows are fed in free pans with stanchions installed.

以上のようにして、本実施形態の乳牛用飼料を用いた乳牛の飼料給与方法によれば、タンパク質分解酵素の酵素活性度が1日あたり600GDU~2400GDUとなるようにしたパイナップル由来飼料が含まれる乳牛用飼料を、乾乳期間中に給与することで、乳牛の分娩後においても尿pH値上昇を抑制することができる。 As described above, according to the feed feeding method for dairy cows using the feed for dairy cows of the present embodiment, the pineapple-derived feed in which the proteolytic enzyme activity is 600 to 2400 GDU per day is included. By feeding dairy cow feed during the dry period, it is possible to suppress an increase in the urine pH value even after parturition of the dairy cow.

<効果試験>
次に、上記の乳牛用飼料及び乳牛の給与方法による効果を確認するために、酵素活性度の異なる3種類のパイナップル粕を乾乳牛に給与し、分娩前後の栄養代謝状態や尿pH値などにどのように影響を与えるかについて試験した。
<Effect test>
Next, in order to confirm the effects of the dairy cow feed and feeding method for dairy cows, three types of pineapple meal with different enzyme activities were fed to dry cows, and the nutrient metabolism before and after parturition and the urine pH value were evaluated. I tested how it works.

なお、飼料成分については、パイナップル粕、TMR調製に使用する配合飼料は、試験期間中に1回成分分析を行った。試験期間中、おおよそ2週間に1回の頻度で給与されたTMRの一部を採取し分析した。また、TMRの設計が変更した場合、使用するサイレージや乾草の調整日または収穫日が変更した場合にも、その都度成分分析を行った。そして、試験方法は次の通りである。 As for feed components, pineapple meal and compound feed used for TMR preparation were analyzed once during the test period. A portion of TMR fed approximately once every two weeks during the study period was collected and analyzed. In addition, when the TMR design was changed, and when the silage or hay preparation date or harvest date was changed, the component analysis was performed each time. And the test method is as follows.

(試験牛)
乾乳牛20頭を平均産次、BCS(ボディコンディションスコア)、分娩時期にばらつきのないよう群分けし、分娩予定日8週前から分娩後3週目まで調査を行った。
(test cow)
Twenty dry cows were grouped so that there was no variation in average calving, BCS (body condition score), and calving time, and the survey was conducted from 8 weeks before the expected calving date to 3 weeks after calving.

(処理区)
5頭×4処理
・パインA区:パイナップル粕A(酵素活性度120,000GDU/18kg袋、1日600GDU/100g)を給与
・パインB区:パイナップル粕B(酵素活性度240,000GDU/18kg袋、1日1200GDU/100g)を給与
・パインC区:パイナップル粕C(酵素活性度480,000GDU/18kg袋、1日2400GDU/100g)を給与
・対照区:無処理
(treatment area)
5 cows × 4 processing ・ Pine A group: Pineapple meal A (enzyme activity 120,000 GDU / 18 kg bag, 600 GDU / 100 g per day) fed ・ Pine B group: Pineapple meal B (enzyme activity 240,000 GDU / 18 kg bag) , 1,200 GDU/100 g per day) were fed. Pine C group: Pineapple meal C (enzyme activity: 480,000 GDU/18 kg bag, 2400 GDU/100 g per day) was fed. Control group: Untreated.

ここで、パイナップル粕A,B,Cは、パイナップル由来飼料「パインMix-Up」(株式会社近藤榮一商店製)に、2400GDU/gのブロメラインを、50g(パインA区)、100g(パインB区)、200g(パインC区)それぞれを添加したものである。なお、GDUとは ゼラチン消化単位である。 Here, pineapple meal A, B, and C are pineapple-derived feed "Pine Mix-Up" (manufactured by Eiichi Kondo Co., Ltd.), 2400 GDU / g of bromelain, 50 g (pine A area), 100 g (pine B area) ), and 200 g (Pine section C). A GDU is a unit of gelatin digestion.

パインA区: 50g×2400GDU/g=120,000GDU/袋
パインB区:100g×2400GDU/g=240,000GDU/袋
パインC区:200g×2400GDU/g=480,000GDU/袋
Pine A section: 50 g x 2400 GDU/g = 120,000 GDU/bag Pine B section: 100 g x 2400 GDU/g = 240,000 GDU/bag Pine C section: 200 g x 2400 GDU/g = 480,000 GDU/bag

・飼料は、朝夕2回(午前5時頃と午後3時半頃)に分けて給与した。
・試験牛は、スタンチョンが設置されたフリーパンで飼養し、飼料給与時にはスタンチョンをかけて他の牛の飼料を盗食できないようにした。
・乾乳期間中に、グラスサイレージ及びコーンサイレージと、乾乳期用配合飼料を混合して調製した混合飼料(TMR)を、乾乳前期(分娩予定日8~3週前まで)は16kg/日、乾乳後期(分娩予定日3週前から分娩日まで)は10kg/日給与した。
- The feed was fed twice in the morning and evening (around 5:00 am and around 3:30 pm).
・The test cows were fed in free pans with stanchions installed, and the stanchions were put on the cows to prevent them from stealing the feed of other cows.
・During the dry period, the mixed feed (TMR) prepared by mixing grass silage and corn silage and the mixed feed for the dry period is 16 kg / 10 kg/day was fed during the late period of dry milk (from 3 weeks before the expected date of delivery to the date of delivery).

・乾乳後期には、前記TMRに加えて、乾乳期用配合飼料を分量が4kg/日となるよう、トップドレスで給与し、パイン区の牛には、パイン区ごとに酵素活性度の異なるパイナップル由来飼料(パイナップル粕を主原料として、カシューナッツ殻、酵素処理パイナップル根茎副産物を混合したもの)100g/日、TMR内に均一になるよう手で混ぜて給与した。
・水、塩(鉱塩セレニクス)、乾草は、対照区、パイン区ともに自由採食とした。
・In the late dry period, in addition to the above TMR, the mixed feed for the dry period was fed in a top dress so that the amount was 4 kg / day, and the enzyme activity of the cows in the pine section was evaluated for each pine section. 100 g/day of different pineapple-derived feeds (a mixture of cashew nut shells and enzymatically treated pineapple rhizome by-products containing pineapple meal as the main raw material) was mixed by hand so as to be uniform in the TMR and fed.
・ Water, salt (mineral salt Serenix), and hay were freely fed in both the control plot and the pine plot.

(試験結果)
表1及び図1~4に示す。
(Test results)
Shown in Table 1 and FIGS.

Figure 0007239794000002
Figure 0007239794000002

・BCS、RFSについて
週1回、朝10時頃にBCSとRFS(ルーメンフィルスコア)のチェックを行った結果を表1にBCS,RFS、尿pH値をしめす。BCSにおいて、分娩時に対照区と比較してパインB区が低い傾向にあった。分娩後3週目のBCSは,対照区と比較してパインA,B区で低いスコアであった。RFSに差はなかった。
- BCS and RFS BCS and RFS (rumen fill score) were checked once a week at around 10:00 am. Table 1 shows the results of BCS, RFS and urine pH values. In the BCS, there was a tendency for the pine B section to be lower than the control section at delivery. The BCS score at 3 weeks after parturition was lower in the pine A and B sections than in the control section. There was no difference in RFS.

・尿pH値について
分娩予定日8週前,3週前、1週前、分娩後0週目及び1週目に、簡易pHメータを用いて尿pH値を測定した。その結果を表1に示す。
- Urinary pH value Urine pH values were measured using a simple pH meter at 8 weeks, 3 weeks, and 1 week before the expected date of delivery, and at 0 and 1 weeks after delivery. Table 1 shows the results.

対照区の平均尿pH値が乾乳時から分娩1週間前までの期間中にやや上昇しているのに対し、どのパイン区も、維持若しくはやや減少していることから、少なくとも乾乳期中のブロメラインを含むパイナップル由来飼料の給与は、尿pH値の上昇を抑制する効果があると認められる。特に、パインC区において効果が大きくなっている。 While the average urine pH value in the control group slightly increased during the period from the dry period to one week before parturition, all pine groups maintained or slightly decreased, indicating that at least during the dry period. It is recognized that feeding pineapple-derived feed containing bromelain has an effect of suppressing an increase in urine pH value. In particular, the effect is large in the pine section C.

・血液性状及び乳量
分娩予定日8週前(乾乳時)、3週前、1週前、分娩後0週目及び1週目に採血し、TP、BUN,総コレステロール(Tーcho)、ケトン体分画、NEFA,Ca,P、Mg、AST、γーGTP、血糖を分析した。また、初乳の乳量とBrix値を記録した。
・Blood properties and milk volume Blood samples were collected 8 weeks before the expected date of delivery (dry milk), 3 weeks, 1 week, and 0 and 1 weeks after delivery. TP, BUN, total cholesterol (T-cho) , ketone body fraction, NEFA, Ca, P, Mg, AST, γ-GTP, blood glucose were analyzed. In addition, the milk yield and Brix value of colostrum were recorded.

図1~4に血液性状及び乳量の推移を示す。なお、パインC区では、分娩後試験牛の1頭が安楽殺となったので、分娩後の統計処理では4頭分のデータを用いている。 Figures 1 to 4 show changes in blood properties and milk yield. In addition, since one of the test cows was euthanized after parturition in Pine C area, data for four cows were used for postpartum statistical processing.

分娩後3週目の血中Ca濃度は、対照区と比較してパインC区の値が高かった。また、3週目の血中P濃度は、対照区と比較してパインA区で低い傾向にあった。また、乳量に差は見られなかった。 The blood Ca concentration at 3 weeks after parturition was higher in the pine C group than in the control group. In addition, the blood P concentration at week 3 tended to be lower in the pine A group than in the control group. In addition, there was no difference in milk yield.

血中Ca濃度は、分娩後3週目で、パインC区で高い値を示すことから、ブロメラインを含むパイナップル由来飼料給与には、Caイオン分泌経路をブロックする(つまり、乳中に移行するCaを減少させ、血中Ca濃度の低下を抑制する)ことが認められた。 Three weeks after parturition, the blood Ca concentration showed a high value in the pineapple C group. and suppresses the decrease in blood Ca concentration).

よって、パインA区~C区を乾乳牛に給与することで、パインを含まない対照区では乾乳時から乾乳時にかけて尿pHが上昇しているところ、パインA区~C区では乾乳時から乾乳時にかけて尿pHの上昇が抑制されており、分娩後においても、尿pH値の上昇が抑制された。特に、パインC区では、尿pH値の上昇を抑制する効果がより大きく、分娩後の血中Ca濃度の低下を防ぐことも認められる。 Therefore, by feeding pine A to C to dry cows, the urine pH increased from dry to dry milk in the control group without pine, while dry milk in pine A to C increased. The increase in urinary pH was suppressed from the time of milking to the time of dry milk, and the increase in urine pH value was also suppressed after parturition. In particular, in the Pine C section, the effect of suppressing the increase in urine pH value is greater, and it is also recognized that the decrease in postpartum blood Ca concentration is prevented.

なお、乾乳期から分娩後3週まで、低Ca血症、ケートシス、入熱、第四胃変異、後産停滞、子宮炎の発生を観察したが、パイン区間には、分娩後の低Ca血症の発症率等に差はみられなかった。 From the dry period to 3 weeks after parturition, hypocalcaemia, ketosis, heat input, mutation of the abomasum, postpartum stagnation, and metritis were observed. No difference was observed in the incidence of hematoma.

また、パインC区で最も効果的であることから、酵素活性度がより高くなるようタンパク質分解酵素を含むパイナップル由来飼料の分量をさらに増加させた飼料とすることで、尿pH値の上昇をさらに抑制することが予測される。ただし、パイナップル由来飼料を増加させすぎると、飼料の味が大きく変化したり、乳牛用飼料全体の栄養バランスが崩れるたりするため、最大でも6kg程度が上限と考えられる。 In addition, since it is most effective in the pineapple section C, the amount of pineapple-derived feed containing proteolytic enzymes is further increased to further increase the enzyme activity, thereby further increasing the urine pH value. expected to be suppressed. However, if the amount of pineapple-derived feed is increased too much, the taste of the feed will change significantly, and the nutritional balance of the whole feed for dairy cows will be disturbed.

以上の試験結果から、上記した乳牛用飼料を用いた乳牛の飼料給与方法を行うことで、タンパク質分解酵素の酵素活性度が1日あたり600GDU以上となるようにしたパイナップル由来飼料が含まれる乳牛用飼料を、乾乳期間中に給与することで、乳牛の分娩後においても尿pH値上昇を抑制することができることが示された。 From the above test results, by performing the feed feeding method for dairy cows using the above-described dairy cow feed, the enzyme activity of the protease is set to 600 GDU or more per day For dairy cows containing pineapple-derived feed It was shown that feeding the feed during the dry period can suppress the increase in urine pH value even after parturition of dairy cows.

以上のとおり、図面を参照しながら本発明の好適な実施形態を説明したが、本発明の趣旨を逸脱しない範囲で、種々の追加、変更または削除が可能である。したがって、そのようなものも本発明の範囲に含まれる。 As described above, preferred embodiments of the present invention have been described with reference to the drawings, but various additions, changes, or deletions can be made without departing from the scope of the present invention. Accordingly, such are also included in the scope of the present invention.

Claims (16)

乾乳期間中に、サイレージや繊維質飼料などの粗飼料と、配合飼料とを混合して調整した混合飼料を給与すること、
乾乳期間中に、タンパク質分解酵素を含むパイナップル由来飼料を給与すること、
前記タンパク質分解酵素は、酵素活性度が1日あたり600GDU以上となるように給与することで、分娩後における尿pHの上昇が抑制されることを特徴とする乳牛の飼料給与方法。
feeding mixed feed prepared by mixing rough feed such as silage and fiber feed with compound feed during the dry period;
feeding a pineapple-derived diet containing proteolytic enzymes during the dry period;
A feed feeding method for dairy cows, wherein the proteolytic enzyme is fed so that the enzyme activity is 600 GDU or more per day, thereby suppressing an increase in urine pH after parturition.
前記タンパク質分解酵素は、酵素活性度が1日あたり600GDU~2400GDUとなるように給与することを特徴とする、
請求項1に記載の乳牛の飼料給与方法。
The proteolytic enzyme is fed so that the enzyme activity is 600 GDU to 2400 GDU per day,
The method of supplying feed to dairy cattle according to claim 1.
前記タンパク質分解酵素は、酵素活性度が1日あたり2400GDU以上となるように給与することを特徴とする、
請求項1に記載の乳牛の飼料給与方法。
The proteolytic enzyme is fed so that the enzyme activity is 2400 GDU or more per day,
The method of supplying feed to dairy cattle according to claim 1.
前記パイナップル由来飼料を、前記混合飼料に均一になるように混ぜて給与する、
請求項1乃至3のいずれか1項に記載の乳牛の飼料給与方法。
The pineapple-derived feed is uniformly mixed with the mixed feed and fed.
A method of feeding a dairy cow according to any one of claims 1 to 3.
前記混合飼料は、前記粗飼料がグラスサイレージ及びコーンサイレージが含まれる粗飼料であり、これを前記配合飼料と混合して調製した混合飼料である、
請求項1乃至4のいずれか1項に記載の乳牛の飼料給与方法。
The mixed feed is a mixed feed prepared by mixing the roughage containing grass silage and corn silage with the mixed feed.
A method of feeding a dairy cow according to any one of claims 1 to 4.
前記パイナップル由来飼料は、パイナップル粕を主原料として、カシューナッツ殻、酵素処理パイナップル根茎副産物を混合したものである、
請求項1乃至5のいずれか1項に記載の乳牛の飼料給与方法。
The pineapple-derived feed is a mixture of cashew nut shells and enzyme-treated pineapple rhizome by-products, with pineapple meal as the main raw material.
A method of feeding a dairy cow according to any one of claims 1 to 5.
前記乾乳期間の後期に前記混合飼料に加えて、乾乳期用配合飼料をトップドレスで給与する、
請求項1乃至6のいずれか1項に記載の乳牛の飼料給与方法。
In the latter half of the dry period, in addition to the mixed feed, a dry period mixed feed is top-dressed,
A method of feeding a dairy cow according to any one of claims 1 to 6.
前記乾乳期間の後期は、前記乾乳期間の前期よりも前記混合飼料の給与量を少なくする、
請求項1乃至7のいずれか1項に記載の乳牛の飼料給与方法。
In the latter half of the dry period, the amount of the mixed feed to be fed is less than in the early period of the dry period.
A method of feeding a dairy cow according to any one of claims 1 to 7.
前記混合飼料は、朝夕2回に分けて給与する、
請求項1乃至8のいずれか1項に記載の乳牛の飼料給与方法。
The mixed feed is fed twice in the morning and evening,
A method of feeding a dairy cow according to any one of claims 1 to 8.
水、塩、乾草は、自由採食とする、
請求項1乃至9のいずれか1項に記載の乳牛の飼料給与方法。
Water, salt and hay are free-feeding.
A method of feeding a dairy cow according to any one of claims 1 to 9.
スタンチョンが設置されたフリーパンで飼養し、飼料給与時にはスタンチョンをかけて他の牛の飼料が盗食をするのを防止する、
請求項1乃至10のいずれか1項に記載の乳牛の飼料給与方法。
The cows are fed in free pans with stanchions installed, and the stanchions are put on the cows when feeding them to prevent other cows from stealing their feed.
A method of feeding a dairy cow according to any one of claims 1 to 10.
乾乳期間中に乳牛に給与することで、分娩後における尿pHの上昇が抑制される飼料であり、
サイレージや繊維質飼料などの粗飼料と、配合飼料とを混合して調製した混合飼料と、タンパク質分解酵素を含むパイナップル由来飼料とを含み、
前記タンパク質分解酵素が、乳牛の1日あたりの飼料に、酵素活性度が600GDU以上となる分量が含まれていることを特徴とする、乳牛用飼料。
It is a feed that suppresses the increase in urine pH after parturition by being fed to dairy cows during the dry period,
Rough feed such as silage and fiber feed, mixed feed prepared by mixing compound feed, and pineapple-derived feed containing proteolytic enzymes,
A feed for dairy cows, wherein the proteolytic enzyme is contained in the daily feed for dairy cows in an amount such that the enzyme activity is 600 GDU or more.
前記タンパク質分解酵素が、乳牛の1日あたりの飼料に、酵素活性度が600GDU~2400GDUとなる分量が含まれていることを特徴とする、
請求項12に記載の乳牛用飼料。
The proteolytic enzyme is contained in the dairy cow's feed per day in an amount such that the enzyme activity is 600 GDU to 2400 GDU,
The feed for dairy cattle according to claim 12.
前記タンパク質分解酵素が、乳牛の1日あたりの飼料に、酵素活性度が2400GDU以上となる分量が含まれていることを特徴とする、
請求項12に記載の乳牛用飼料。
The proteolytic enzyme is contained in the dairy cow's feed per day in an amount such that the enzyme activity is 2400 GDU or more,
The feed for dairy cattle according to claim 12.
前記混合飼料は、前記粗飼料がグラスサイレージ及びコーンサイレージが含まれる粗飼料であり、これを配合飼料と混合して調製した混合飼料である、
請求項12乃至14のいずれか1項に記載の乳牛用飼料。
The mixed feed is a mixed feed prepared by mixing the roughage containing grass silage and corn silage with a mixed feed.
Dairy cow feed according to any one of claims 12 to 14.
前記パイナップル由来飼料は、パイナップル粕を主原料として、カシューナッツ殻、酵素処理パイナップル根茎副産物を混合したものである、
請求項12乃至15のいずれか1項に記載の乳牛用飼料。
The pineapple-derived feed is a mixture of cashew nut shells and enzyme-treated pineapple rhizome by-products, with pineapple meal as the main raw material.
A dairy cow feed according to any one of claims 12 to 15.
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JP3413169B2 (en) 2000-04-03 2003-06-03 株式会社近藤榮一商店 Fattening method of beef or pork
JP3122140U (en) 2006-03-23 2006-06-01 中部飼料株式会社 Cattle supplements
JP2016082961A (en) 2014-10-28 2016-05-19 株式会社近藤榮一商店 Sleep-inducing milk and production method therefor, and feedstuff for milk cow
JP2017169451A (en) 2016-03-18 2017-09-28 株式会社近藤榮一商店 Sleep inducing milk and manufacturing method thereof and feedstuff for dairy cattle

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JP3413169B2 (en) 2000-04-03 2003-06-03 株式会社近藤榮一商店 Fattening method of beef or pork
JP3122140U (en) 2006-03-23 2006-06-01 中部飼料株式会社 Cattle supplements
JP2016082961A (en) 2014-10-28 2016-05-19 株式会社近藤榮一商店 Sleep-inducing milk and production method therefor, and feedstuff for milk cow
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