JP7053231B2 - Algae, nutritional supplements, nutritional component supplementation compositions and methods for producing nutritional components - Google Patents

Description

IPOD FERM P-22338

The present invention relates to algae, nutritional supplements, nutritional component supplementation compositions, and methods for producing nutritional components.

From the viewpoint of depletion of petroleum resources and reduction of greenhouse gases, there is a great deal of interest in the development of biomass fuels, and research is currently being conducted to produce fuel oils from microalgae (see, for example, Patent Document 1).

On the other hand, most microalgae produce ω3 or ω6 unsaturated fatty acids such as linolenic acid, docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), and functional substances such as astaxanthin. These have various functionalities. Therefore, attention is being paid to the use of microalgae in health and functional foods and pharmaceuticals.
In addition, microalgae species that produce biofuel oils or functional substances have been reported so far (see, for example, Non-Patent Document 1).

Japanese Unexamined Patent Publication No. 9-009953

Cuellar-Bermudez P. S. et al., “Extraction and purification of high-value metabolites from microalgae: essential lipids, astaxanthin and phycobiliproteins.”, Microbial Biotechnology, 8, Minireview, p190-209, 2014.

Currently, the microalgae that are industrially put into practical use are limited to several kinds of algae, and there is a demand for new microalgae species that produce hydrocarbons, functional oils, or functional substances.

The present invention has been made in view of the above circumstances, and is a novel microalgae that can be industrially used, a novel nutritional supplement using the algae, a nutritional component supplementing composition containing the nutritional supplement, and the microalgae. Provided is a method for producing a nutritional component using.

As a result of diligent research to achieve the above object, the present inventors have found that Scenedesmus sp. AB-1C strain (FERM P-22338) is a hydrocarbon, a functional oil such as EPA, and a functional oil such as EPA. They have found that they produce various useful substances such as carotenoids such as astaxanthin, and have completed the present invention.

That is, the present invention includes the following aspects.
The alga according to the first aspect of the present invention is Scenedesmus sp. AB-1C strain (FERM P-22338).

The nutritional supplement according to the second aspect of the present invention contains the Senedesmus sp. AB-1C strain and its extract according to the first aspect, and the Senedesmus sp. AB-1C strain and its extract are eicosapentaenoene as a nutritional component. Contains acid.
In the nutritional supplement according to the second aspect, the Senedesmus SP AB-1C strain and its extract may further contain astaxanthin as a nutritional component.
In the nutritional supplement according to the second aspect, the content of astaxanthin may be 300 mg or more and 600 mg or less per 100 g of the dry weight of the Senedesmus SP AB-1C strain.

The nutritional component supplementing composition according to the third aspect of the present invention contains the nutritional supplement according to the second aspect.
In the nutritional component supplementing composition according to the third aspect, one or more selected from the group consisting of carotenoids other than astaxanthin , linoleic acid and α-linolenic acid may be further contained as the nutritional component.
The nutritional component supplement composition according to the third aspect may be a food , a functional food, or a dietary supplement .
The nutritional component supplementing composition according to the third aspect may be feed or pet food.
The nutritional component supplementing composition according to the third aspect may be a cosmetic product.

The method for producing a nutritional component according to the fourth aspect of the present invention is a method comprising a culture step for culturing the Senedesmus sp. AB-1C strain according to the first aspect.
The method for producing a nutritional component according to the fourth aspect further comprises, after the culture step, a crushing step of crushing the Senedesmus SP AB-1C strain to obtain a cell crushed product, and a crushing step of obtaining the nutritional component from the cell crushed product. A separation step for separation may be provided in this order.
The nutritional component may be one or more selected from the group consisting of eicosapentaenoic acid, carotenoids, linoleic acid and α-linolenic acid.
The carotenoids may be astaxanthin.

According to the above aspect, a novel microalgae that can be industrially used, a novel nutritional supplement using the algae, a nutritional component supplementing composition containing the nutritional supplement, and a method for producing a nutritional component using the microalgae are provided. can do.

It is a micrograph of a scenedesmus SP AB-1C strain. The image above is a photomicrograph in the bright field. The image below is a fluorescence micrograph. The molecular phylogenetic tree of the genus Senedesmus and the genus Coellastrella based on the base sequence of 18S ribosome is shown. The known Senedesmus SP Ki4 strain and JB11 strain are surrounded by a dotted line, and the AB-1C strain is surrounded by a solid line.

≪Algae≫
The algae according to one embodiment of the present invention is Scenedesmus sp. AB-1C strain (FERM P-22338) (hereinafter, may be referred to as "AB-1C strain").

The genus Sphaeropleales is taxonomically classified into the phylum Green algae (Chlorophyta), the green algae (Chlorophyceae), the order Sphaeropleales, and the scenedesmaceae. The genus Senedesmus is also called the genus Scenedesmus. As the algae belonging to the Scenedesmaceae family, in addition to the genus Senedesmus, the genus Desmodesmus and the genus Actodesmus are known. Algae belonging to the Scenedesmaceae family grow widely in ponds and lakes in nature.

The AB-1C strain belongs to the genus Senedesmus and produces various useful substances such as hydrocarbons, functional oils such as EPA, and carotenoids such as astaxanthin. Conventionally, it has been known that known algae belonging to the genus Scenedesmus produce useful substances as described above, but those producing astaxanthin at a particularly high concentration have not been known. Among the genus Scenedesmus, those known to produce carotenoids together with functional oils such as hydrocarbons and EPAs are Scenedesmus obliqui and Scenedesmus komarekii. Only. Therefore, the AB-1C strain is distinguished from other algae belonging to the genus Senedesmus in that it produces astaxanthin at a high concentration together with functional oils such as hydrocarbons and EPA.

The AB-1C strain is a unicellular green alga isolated from the estuary surface water of the Jinzu River in Toyama Bay, Toyama Prefecture, Japan. The AB-1C strain was issued on June 27, 2017, under the accession number NITE P-22338, the National Institute of Technology and Evaluation Patent Microorganisms Depositary Center (2-5-8 Kazusakamatari, Kisarazu City, Chiba Prefecture, Japan). Has been deposited in.

The AB-1C strain has a chlorophyll a at the initial stage of culture, and therefore exhibits a green color. On the other hand, since carotenoids such as astaxanthin are produced and accumulated in the cells, they turn orange as the culture time increases.
The shape and size of the cells are elliptical spherical or spherical, and cells of different sizes are mixed. The difference in shape is presumed to be due to the cell cycle, and the spherical one is considered to be the mother cell before division.
Specifically, in the case of an elliptical spherical cell, the average major axis is about 10.9 μm and the average minor axis is about 8.8 μm. The spherical shape has an average diameter of about 14.6 μm.

The AB-1C strain grows in two divisions. The AB-1C strain preferably grows in the presence of trace amounts of heavy metal ions. Examples of the heavy metal ion include Cu ²⁺ , Zn ²⁺ , Mn ²⁺ , Co ²⁺ , Mo ²⁺ and the like. The optimum temperature is about 25 ° C., and the optimum pH is around 8.4.

As a result of molecular phylogenetic analysis based on the base sequence of 18S ribosomal DNA, the AB-1C strain was classified into the genus Senedesmus. The nucleotide sequence of 18S ribosomal DNA of AB-1C strain is shown in SEQ ID NO: 1.

Examples of the algae that are closely related to the AB-1C strain include algae having a base sequence of 18S ribosomal DNA having 90% or more identity with SEQ ID NO: 1. The identity of the base sequence of the 18S ribosomal DNA possessed by the algae with the base sequence set forth in SEQ ID NO: 1 is preferably 95% or more, more preferably 97% or more, and more preferably 98% or more. It is more preferable, and 99% or more is particularly preferable.

In addition, the identity (homosphere) between the base sequences is obtained by juxtaposing the two base sequences with a gap in the portion corresponding to the insertion and deletion so that the corresponding bases match most. It is calculated as the ratio of matching bases to the entire base sequence excluding the gap of. The identity between the base sequences can be determined by using various homology search software known in the art. For example, the identity value of the base sequence can be obtained by calculation based on the alignment obtained by the known homology search software BLASTn.

Further, the base sequence of 18S ribosomal DNA possessed by algae can be obtained by a known method. For example, DNA is extracted from target algae cells by a known method, and a DNA fragment of 18S ribosomal DNA is amplified by a PCR method or the like. Next, by analyzing the base sequence of the amplified DNA fragment with a DNA sequencer, the base sequence of 18S ribosomal DNA of the target algae can be obtained. Examples of the primer for amplifying 18S ribosomal DNA include the primers used in the examples of the present specification.

Further, as the algae of the present embodiment, a mutant strain of AB-1C strain is also mentioned as a suitable example.
As used herein, the term "variant strain" means that the genome of the original algae strain (including nuclear genome, chloroplast genome, mitochondrial genome, hereinafter the same) is spontaneously or artificially mutated. Means an algae strain. The artificial method for causing a mutation in the genome is not particularly limited, and examples thereof include chemical treatment with ultraviolet irradiation, irradiation, nitrite and the like; genetic engineering methods such as gene transfer and genome editing.

In the present specification, the "variant strain of AB-1C strain" is an algae strain in which the genome of AB-1C strain is mutated, and has a nutritional component similar to that of AB-1C strain. Refers to an algae strain having a composition. For example, the total carotenoid content may be 400 mg or more and 1000 mg or less, 450 mg or more and 900 mg or less, or 500 mg or more and 800 mg or less per 100 g of the dry weight of the AB-1C strain.

Further, for example, the content of astaxanthin may be 50 mg or more and 800 mg or less, 300 mg or more and 600 mg or less, or 350 mg or more and 550 mg or less per 100 g of the dry weight of the AB-1C strain.

As the mutant strain of AB-1C strain, the ratio of mutation to the whole genome of AB-1C strain is preferably 10% or less, more preferably 5% or less, and 3% or less. It is more preferably present, and particularly preferably 2% or less or 1% or less.

The algae of this embodiment can grow under medium temperature environment (15 to 35 ° C.) and weakly basic (pH 7.8 to 8.8) conditions. Therefore, it is possible to change the culture conditions according to the region and season, and it is suitable for outdoor mass culture.
Furthermore, it has high salt tolerance and can grow even under high salt conditions such as seawater (500 mM NaCl, etc.). Further, the light intensity can be grown in the range of 3500 to 5000 lux, and can be grown even under strong light.

The algae of the present embodiment include hydrocarbons that can be fuel oils, and nutrients such as amino acids, vitamins (particularly carotenoids such as astaxanthin), proteins, lipids (particularly functional oils such as EPA), and dietary fiber. Rich in ingredients.

In particular, as shown in Examples described later, astaxanthin is contained in a high concentration even as compared with the Monorapidium sp. GK-12 strain known as a conventional astaxanthin-producing algae. It has been confirmed. In addition, various functional oils (EPA, α-linolenic acid and linoleic acid) and carotenoids (astaxanthin, canthaxanthin, β-carotene and lutein) are compared with the conventionally used algae (hematococcus). Has been confirmed to contain.

In the present specification, the "functional oil" means an edible oil having functions such as a neutral fat lowering effect, a cognitive function improving effect, and a blood cholesterol lowering effect. Specific examples of the functional oil include ω3 fatty acids such as docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), α-linolenic acid, and ω6 fatty acids such as linolenic acid and arachidonic acid. Can be mentioned.

The algae of this embodiment contain EPA, α-linolenic acid, linoleic acid and the like as functional oils, as shown in Examples described later.

Further, in the present specification, "carotenoids" means a group of natural pigments showing yellow, orange, red and the like. Specific examples of carotenoids include carotenes such as α-carotene, β-carotene, γ-carotene, δ-carotene, lycopene, and ε-carotene; , Xanthophylls such as violaxanthin and the like.
The algae of this embodiment contain astaxanthin, canthaxanthin, β-carotene, lutein and the like as carotenoids, as shown in Examples described later.

The algae of this embodiment contain a hydrocarbon that can be a fuel oil. Therefore, the biomass fuel can be produced by using the algae of the present embodiment.

Furthermore, the algae of the present embodiment contain various nutritional components such as functional oils such as EPA and carotenoids such as astaxanthin. Therefore, when the algae of the present embodiment are ingested by humans or non-human animals as a nutritional supplement described later, the above-mentioned nutritional components can be absorbed into the body. In addition, an extract containing a nutritional component can be appropriately prepared from the algae of the present embodiment.

The algae of the present embodiment can be cultivated using a medium for culturing microalgae. The medium is not particularly limited, and examples thereof include an inorganic salt medium containing a nitrogen source, a phosphorus source, trace elements (zinc, boron, cobalt, copper, manganese, molybdenum, iron, etc.) and the like. For example, examples of the nitrogen source include ammonium salts, nitrates, nitrites, ureas, amines and the like. Further, examples of the phosphorus source include phosphates and the like.

Specifically, as the above-mentioned medium, for example, SGI medium (Reference 1: SAGER R, GRANICK S. “Nutritional studies with Chlamydomonas reinhardi.”, Ann NY Acad Sci., Vol. 56, No. 5, p.831- 838, 1953.), TAP Medium (Reference 2: Gorman, D.S. and Levine, R.P., “Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.”, Proc Natl Acad Sci USA, Vol. 54 , No. 6, p1665-1669, 1965.).

As described above, the algae of the present embodiment can be grown under a relatively wide range of culture conditions. As the pH condition, pH 7.8 to 10.0 can be exemplified, and pH 8.0 to 8.6 is preferable.

As the temperature condition, 15 to 35 ° C. can be exemplified, and 20 to 30 ° C. is preferable.

As the light intensity, 3500 to 5000 lux can be exemplified, and 4000 to 4500 lux is preferable. When culturing outdoors, it may be cultivated in sunlight. When culturing indoors, culturing may be performed with continuous light, or a light-dark cycle (12L: 12D, etc.) may be provided.

As described above, the algae of the present embodiment contain abundant nutritional components and are suitable for outdoor mass culture, and therefore can be industrially used as a nutritional supplement described later. In addition, the nutritional component can be extracted from the algae of the present embodiment, and the nutritional component can be absorbed into the body even when ingested as it is by a human or a non-human animal.

≪Nutrition ≫
The nutritional supplement according to one embodiment of the present invention includes the Senedesmus sp. AB-1C strain of the above-mentioned embodiment or an extract thereof.

The algae contained in the nutritional supplement of the present embodiment are the algae of the above-mentioned embodiment. That is, the AB-1C strain and its mutant strain (hereinafter referred to as "this algae"). Preferred examples of the algae contained in the nutritional supplement of the present embodiment are the same as those exemplified above.
The algae can be used as the nutritional supplement of the present embodiment by culturing and growing them in an appropriate medium, recovering them by a known method such as centrifugation or filtration, and appropriately washing and drying them. ..

In addition, the nutritional supplement of the present embodiment may contain an extract of the algae in place of the algae or together with the algae.
As used herein, the term "extract of the algae" refers to the cells of the algae that have been physically or chemically treated to extract the intracellular components. For example, the extract of the algae may be a cell crushed product obtained by disrupting the cells of the algae by physical treatment or chemical treatment. Further, the extract of the algae may be one in which the cell crushed product is concentrated, or the product in which the solid content is removed from the cell crushed product, and some components are added from the cell crushed product. It may be separated.

The method of physical treatment or chemical treatment of cells is not particularly limited, and methods generally used for cell disruption can be used. Examples of the physical treatment include cell disruption using glass beads, a mortar, ultrasonic treatment, a French press, a homogenizer, and the like. Examples of the chemical treatment include a neutralization treatment, a hypotonic treatment, a freeze-thaw treatment, and the like.
When concentrating the cell disrupted product, the concentration method is not particularly limited, and a generally used concentration method may be used. Examples of the method for concentrating the cell disrupted product include drying, freeze-drying, vacuum-drying and the like.
Further, when the solid content is removed from the cell crushed product, the method for removing the solid content is not particularly limited, and a method generally used for removing the solid content or the like can be used. Examples of the method for removing the solid content include filtration, centrifugation and the like.
When separating a part of the components from the cell disrupted product, the separation method is not particularly limited, and a method generally used for separation and purification of biochemical substances can be used. Examples of the separation method include salting out, dialysis, solvent extraction, adsorption, various types of chromatography (gas chromatography, thin layer chromatography, ion exchange chromatography, high performance liquid chromatography, adsorption chromatography, etc.) and the like. These methods may be used alone or in combination of two or more treatments. However, those purified into a single component are excluded from the "extract of this algae". The extract of this alga preferably contains 2 or more types of cellular components of this alga, more preferably 4 or more types, and further preferably 6 or more types.

As described above, since the algae contain a large amount of nutritional components such as functional oils and carotenoids, the extract of the algae also contains a large amount of nutritional components such as functional oils and carotenoids. Therefore, it can be used as a nutritional supplement of the present embodiment.

The nutritional supplement of the present embodiment may contain other components as appropriate in addition to the algae or an extract thereof. Examples of other components include, but are not limited to, pharmaceutically acceptable carriers and the like.
The "pharmaceutically acceptable carrier" means a carrier that does not inhibit the function of the nutritional component contained in the algae and does not show substantial toxicity to the administration target thereof.
Further, "does not show substantial toxicity" means that the component does not show toxicity to the administration subject at the dose normally used.
The pharmaceutically acceptable carrier is not particularly limited, and is, for example, an excipient, a binder, a disintegrant, a lubricant, an emulsifier, a stabilizer, a diluent, an oily base, a thickener, and an antioxidant. , Reducing agents, oxidizing agents, chelating agents, solvents and the like. As the pharmaceutically acceptable carrier, one type may be used alone, or two or more types may be used in combination.

The content of the algae or its extract in the nutritional supplement of the present embodiment is not particularly limited, and can be appropriately selected in the range of, for example, 1 to 100% by mass. The content of the algae or its extract in the nutritional supplement of the present embodiment is preferably 50 to 100% by mass, more preferably 60 to 99% by mass, still more preferably 70 to 99% by mass.
The algae or its extract can be appropriately mixed with other components to form dry powder, granules, tablets, jellies, liquids, capsules and the like according to a conventional method.

Since the nutritional supplement of the present embodiment contains a large amount of nutritional components such as functional oils and carotenoids, it can be used as a nutritional supplement for humans and animals other than humans. Examples of the nutritional component supplied by the nutritional supplement of the present embodiment include amino acids, vitamins (particularly carotenoids), proteins, lipids (particularly functional oils), dietary fiber and the like. Above all, the nutritional supplement of the present embodiment is suitably used for supplementing functional oils (EPA, α-linolenic acid, linoleic acid, etc.) and carotenoids (astaxanthin, canthaxanthin, β-carotene, lutein, etc.). be able to.
The nutritional supplement of the present embodiment can be suitably used, in particular, for supplementing EPA and astaxanthin.

Moreover, the nutritional supplement of this embodiment may be blended and used in the composition for supplementing nutritional components described later. By blending the nutritional supplement of the present embodiment, a composition rich in nutritional components such as functional oils and carotenoids can be prepared.

≪Nutrition ingredient supplement composition≫
The nutritional component supplementing composition according to one embodiment of the present invention contains the nutritional supplement of the above embodiment.

As used herein, the term "nutrition component supplementing composition" refers to a composition used by a human or a non-human animal to take in a nutritional component into the body. The uptake of nutritional components into the body may be oral or parenteral.

The nutritional components included in the nutritional component supplementing composition of the present embodiment include functions of amino acids, vitamins (particularly carotenoids such as astaxanthin), proteins, lipids (particularly EPA, α-linolenic acid, linoleic acid and the like). (Sexual oil) and dietary fiber. These components are nutritional components contained in a large amount of the above-mentioned nutritional supplements. Among these, the nutritional component supplementing composition of the present embodiment is characterized in that it contains a large amount of functional oils and carotenoids.
In particular, functional oils are characterized by containing a large amount of EPA, and carotenoids are characterized by containing a large amount of astaxanthin.
For example, EPA is known to have a triglyceride lowering effect. In addition, astaxanthin is known to have an antioxidant effect.
Therefore, by ingesting the nutritional component supplementing composition of the present embodiment, the physical condition improving effect and the like possessed by the nutritional component as described above can be obtained.

The nutritional component supplementing composition of the present embodiment is not particularly limited as long as it is used by a human or a non-human animal to take in the nutritional component into the body. Examples of the nutritional component supplementing composition of the present embodiment include foods, feeds, pet foods, cosmetics and the like.

<Food>
The nutritional component supplement composition of the present embodiment may be a food.
When the nutritional component supplementing composition of the present embodiment is a food product, the above-mentioned nutritional supplement may be added to the food product as a food additive. By adding the above-mentioned nutritional supplement to a food, it is possible to prepare a food product in which the nutritional component contained in the above-mentioned nutritional supplement is fortified. Therefore, the food additive according to the embodiment of the present invention includes the algae or an extract thereof.
The food of the present embodiment can be produced according to a known method according to the type of food by adding the above-mentioned nutritional supplement to the food raw material and adding other food additives as appropriate.

In the food of the present embodiment, the type of food is not particularly limited. Foods include, for example, various noodles such as buckwheat, udon, harusame, Chinese noodles, instant noodles, cup noodles; carbohydrates such as bread, wheat flour, rice flour, hot cakes, mashed potatoes; green juice, soft drinks, carbonated drinks, etc. Beverages such as nutritional drinks, fruit drinks, vegetable drinks, lactic acid drinks, milk drinks, sports drinks, tea and coffee; bean products such as tofu, okara and natto; various soups such as curry roux, stew roux and instant soup; ice cream Cold confectionery such as cream, ice sherbet, shaved ice; confectionery such as candy, cookies, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, and other baked confectionery; Fisheries or processed livestock foods such as; processed milk, fermented milk, butter, cheese, yogurt and other dairy products; salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing and other fats and oils and fat processed foods; sauces, dressings , Miso, soy sauce, sauce and other seasonings; various retort foods, sprinkles, other processed foods such as pickles, etc. can be mentioned, but are not limited thereto.

In the above-mentioned foods, the content of the above-mentioned nutritional supplement is not particularly limited, and the content may be appropriately set according to the type of food. For example, in consideration of the flavor of the food, the content of the above-mentioned nutritional supplement in the food may be 0.01 to 30% by mass as the content of the algae or its extract. From the viewpoint of food flavor and the like, 0.05 to 20% by mass is preferable, 0.1 to 15% by mass is more preferable, 0.1 to 10% by mass is further preferable, and 0.1 to 5% by mass is particularly preferable. preferable.

Alternatively, the nutritional supplement composition of the present embodiment may be a functional food or a dietary supplement. The functional food or dietary supplement may be in the form of a general food as described above, or may be in the form of a dry powder, granules, tablets, jellies, drinks and the like. In this case, the above-mentioned nutritional supplement and other components may be mixed as appropriate to form a dry powder, granules, tablets, jelly preparation, drink preparation or the like according to a conventional method.
The other components are not particularly limited, and examples thereof include pharmaceutically acceptable carriers. Examples of the pharmaceutically acceptable carrier include the same carriers as those mentioned in the above-mentioned << nutritional supplement >>. Further, in order to improve the flavor and the like, sweeteners, flavoring agents, various seasonings, flavors, oils and fats, other food additives and the like may be used as other ingredients. As for the other components, one type may be used alone, or two or more types may be used in combination.

In the above-mentioned functional foods or dietary supplements, the content of the above-mentioned nutritional supplements is not particularly limited, and the content may be appropriately set according to the type of the functional food or the dietary supplement. For example, when the functional food or dietary supplement is in the form of dry powder, granules, tablets, etc., the content of the above-mentioned nutritional supplement in the functional food or dietary supplement is the content of the algae or its extract. The amount is exemplified by 0.1 to 99% by mass. From the viewpoint of efficient supplementation of flavor and nutritional components, 1 to 90% by mass is preferable, 10 to 85% by mass is more preferable, 20 to 85% by mass is further preferable, and 25 to 85% by mass is particularly preferable. When the functional food or dietary supplement is in the form of a jelly or drink, the content of the above-mentioned nutritional supplement in the functional food or dietary supplement is the content of the algae or its extract. , 0.05-80% by mass is exemplified. From the viewpoint of efficient supplementation of flavor and nutritional components, 1 to 75% by mass is preferable, 10 to 70% by mass is more preferable, 15 to 70% by mass is further preferable, and 20 to 70% by mass is particularly preferable.

The food of the present embodiment can be ingested in order to efficiently replenish the above-mentioned nutritional components contained in the algae.

<Feed, pet food>
The nutritional component supplementing composition of the present embodiment may be feed or pet food.
When the nutritional component supplementing composition of the present embodiment is a feed or pet food, the above-mentioned nutritional supplement may be added to the feed or pet food as a feed additive or pet food additive. By adding the above-mentioned nutritional supplement to the feed or pet food, a feed or pet food enriched with the nutritional components contained in the above-mentioned nutritional supplement can be prepared. Therefore, the feed additive or pet food additive according to the embodiment of the present invention includes the algae or an extract thereof.

In the feed or pet food of the present embodiment, the above-mentioned nutritional supplement is added to the feed raw material or pet food raw material, and other feed additives or pet food additives are appropriately added, depending on the type of feed raw material or pet food. It can be manufactured according to a known method.

The type of animal to which the feed or pet food of this embodiment is given is not particularly limited. Examples thereof include livestock (cattle, pigs, chickens, horses, sheep, goats, etc.), fish, shellfish, pet animals (dogs, cats, hamsters, rabbits, parakeets, tropical fish, reptiles, amphibians, insects, etc.). , Not limited to these.

In the feed or pet food of the present embodiment, the content of the above-mentioned nutritional supplement is not particularly limited, and the content may be appropriately set according to the type of feed or pet food. For example, the content of the above-mentioned nutritional supplement in feed or pet food is preferably 0.01 to 90% by mass, preferably 0.1 to 80% by mass, as the content of the algae or its extract. 70% by mass is more preferable, and 1 to 60% by mass is particularly preferable.

The feed or pet food of the present embodiment can be used to efficiently supply the animal with the above-mentioned nutritional components contained in the algae.

<Cosmetics>
The nutritional component supplementing composition of the present embodiment may be a cosmetic product.
When the nutritional component supplementing composition of the present embodiment is a cosmetic product, the above-mentioned nutritional supplement may be added to the cosmetic product as a cosmetic additive. By adding the above-mentioned nutritional supplement to the cosmetic product, it is possible to prepare a cosmetic product containing the nutritional component contained in the above-mentioned nutritional supplement. Therefore, the cosmetic additive according to the embodiment of the present invention includes the algae or an extract thereof.

The cosmetic product of the present embodiment can be produced by mixing the above-mentioned nutritional supplement with other components as appropriate and according to a known method according to the type of cosmetic product.
The other components are not particularly limited, and examples thereof include pharmaceutically acceptable carriers. Examples of the pharmaceutically acceptable carrier include the same carriers as those mentioned in the above-mentioned "<< nutritional supplement >>". Further, a material known as a cosmetic additive may be used as another ingredient. As for the other components, one type may be used alone, or two or more types may be used in combination.

In the cosmetics of the present embodiment, the types of cosmetics are not particularly limited, and examples thereof include basic cosmetics such as cosmetics, milky lotions, lotions, creams, gels, sunscreens, packs, masks, and beauty liquids; foundations, makeups, etc. Makeup cosmetics such as foundations, lipsticks, lip glosses, cheeks; cleaning agents such as face wash, body shampoo, cleansing agent; hair cosmetics such as shampoo, rinse, hair conditioner, treatment, hair conditioner; body powder, body lotion Examples include, but are not limited to, cosmetics for the body.

In the cosmetics of the present embodiment, the content of the above-mentioned nutritional supplement is not particularly limited, and the content may be appropriately set according to the type of cosmetics. For example, in consideration of the feeling of use of cosmetics, the content of the above-mentioned nutritional supplement in cosmetics may be 0.01 to 30% by mass as the content of the algae or its extract. From the viewpoint of the usability of cosmetics, 0.1 to 20% by mass is preferable, 0.1 to 15% by mass is more preferable, 0.1 to 10% by mass is further preferable, and 0.1 to 5% by mass is preferable. Especially preferable.

The cosmetic product of the present embodiment can be applied to the skin or hair in order to supply the above-mentioned nutritional components contained in the algae to the skin or hair.

≪Manufacturing method of nutritional components≫
The method for producing a nutritional component according to an embodiment of the present invention is a method comprising a culture step of culturing the Senedesmus SP AB-1C strain of the above-described embodiment.
Hereinafter, the process of the manufacturing method of the present embodiment will be described.

<Culture process>
The culturing step is a step of culturing the algae.

Preferable examples of the algae used in this step include the same as those described in the above-mentioned "<< Algae >>".
In addition, the culture step can be performed by the method described in the above-mentioned "<< Algae >>".

The production method of the present embodiment further comprises, after the culture step, a crushing step of crushing the Senedesmus SP AB-1C strain to obtain a cell crushed product, and a separation step of separating a nutritional component from the cell crushed product. , May be provided in this order.

<Crushing process>
The crushing step is a step of crushing the cells of this algae to obtain a cell crushed product.

The method for disrupting the cells of this alga is not particularly limited, and a known method can be used. Examples of the cell crushing method include physical treatments such as glass beads, mortars, ultrasonic treatments, French presses, homogenizers; and chemical treatments such as neutralization treatments, hypotonic treatments, and freeze-thaw treatments. These treatments may be performed alone or in combination of two or more types of treatments.

Examples of the neutralization treatment method include a method of immersing the cells of this algae in a neutralizing solution having a pH of about 7 to 10. Since the algae are adapted to the pH in the acidic range, the cells are destroyed by immersing them in a neutral to basic neutralizing solution. The composition of the neutralizing solution is not particularly limited, but for example, a buffer solution such as a phosphate buffer solution or a Tris buffer solution can be used. The time for immersing the cells in the neutralizing solution may be such that the cells are destroyed, and for example, about 10 to 30 minutes is exemplified.

Examples of the hypotonic treatment method include a method of immersing the cells of this alga in a hypotonic solution such as water. Since the cell wall of this alga is weak or thin, the cells burst when immersed in a hypotonic solution. The composition of the hypotonic liquid is not particularly limited, and may be any hypotonic liquid to the extent that the cells of this alga burst. Examples of the hypotonic solution include water, a low-concentration buffer solution, and the like. The time for immersing the cells in the hypotonic solution may be such that the cells rupture, and for example, about 1 to 30 minutes is exemplified.

Examples of the method of freeze-thaw treatment include a method in which the cells of this alga are subjected to a freeze-thaw cycle at least once. The number of freeze-thaw cycles is not particularly limited, and may be any number as long as the cells of this alga are crushed. The number of freezing and thawing cycles is exemplified by, for example, about 1 to 5 times. Each time of freezing and thawing is not particularly limited, and for example, about 10 to 30 minutes each is exemplified.

By crushing the cells of this alga by the method as described above, a cell crushed product of this alga can be obtained.

<Separation process>
The separation step is a step of separating nutritional components from the cell disrupted product of this algae.

In this step, the nutritional component to be separated is not particularly limited as long as it is the nutritional component of the algae. As described in the above-mentioned "<< nutritional supplement >>", this alga contains a large amount of nutritional components such as amino acids, vitamins (particularly carotenoids), proteins, lipids (particularly functional oils), and dietary fiber. .. Therefore, examples of the nutritional components to be separated in this step include at least one selected from the group consisting of functional oils and carotenoids.

Among the nutritional components, examples of the functional oil include at least one selected from the group consisting of EPA, α-linolenic acid and linoleic acid. Preferably, the functional oil includes EPA.
In addition, examples of carotenoids include at least one selected from the group consisting of astaxanthin, canthaxanthin, β-carotene and lutein. Preferably, astaxanthin is mentioned as a carotenoid.

The nutritional components separated in this step may be one kind or two or more kinds. Further, it may be separated according to the type of functional oil (EPA, α-linolenic acid, linoleic acid, etc.), carotenoids (astaxanthin, canthaxanthin, β-carotene, lutein, etc.).

The method for separating the nutritional component from the cell disruption is not particularly limited, and an appropriate method may be selected according to the type of the nutritional component. As a method for separating nutritional components, a method generally used for separation / purification of biochemical substances can be appropriately combined and used. Separation methods include, for example, centrifugation, washing, salting, dialysis, recrystallization, reprecipitation, solvent extraction, adsorption, concentration, filtration, gel filtration, ultrafiltration, and various types of chromatography (gas chromatography, thin layer chromatography). (Graphic, ion exchange chromatography, high-speed liquid chromatography, adsorption chromatography, affinity chromatography, etc.) and the like), but are not limited thereto.

<Arbitrary process>
The production method of the present embodiment may include other steps in addition to the culture step, the crushing step and the separation step. Examples of other steps include a step of recovering the algae from the culture solution (recovery step), a step of washing the cells of the algae (washing step), and the like. These steps can be performed after the culture step described above and before the crushing step.

The recovery step can be performed by a known method such as filtration or centrifugation. The washing step can be performed by suspending the cells in a washing solution (buffer solution or the like) having a pH of 0.1 to 5.0, and then recovering the cells from the washing solution by a method such as filtration or centrifugation.

The nutritional components produced by the production method of the present embodiment can be used for various uses such as nutritional supplements, foods, feeds, pet foods, cosmetics, pharmaceuticals, and reagents.

Hereinafter, the present invention will be described with reference to Examples, but the present invention is not limited to the following Examples.

[Example 1]
1. 1. Isolation of new microalgae The estuary surface water was sampled at the Jinzu River in Toyama Bay, Toyama Prefecture, Japan. Next, the estuary surface water was filtered under reduced pressure with a 0.45 μm polycarbonate membrane filter, and microorganisms containing microalgae were captured on the filter. Next, the whole filter was placed in a culture medium containing KWSW medium, SGI medium (Sager & Granic Medium I), IMK medium and Dunaliella medium, and cultured under a white fluorescent lamp while aerating. The composition of the KWSW medium is shown in Table 1 below, the composition of the SGI medium is shown in Table 2 below, the composition of the IMK medium is shown in Table 3 below, and the composition of the medium for Dunaliella is shown in Table 4 below.

As a result, microalgae proliferated, and a part of the culture solution that became turbid in green or brown was applied on the 1.5% (w / v) agar medium prepared in each of the above media and cultured under a white fluorescent lamp. bottom. The microalgaes applied on the agar medium grew, and the individual colonies formed were isolated to select and isolate the microalgaes (single species). As a result, the AB-1C strain was isolated.

Next, observation of the AB-1C strain under a microscope (manufactured by Olympus Corporation, BX51N-33-PH) was performed (magnification: 540 times). A micrograph of the AB-1C strain is shown in FIG. The image above is a photomicrograph in the bright field. The image below is a fluorescence micrograph.

From FIG. 1, it was confirmed that the morphology of the cells of the AB-1C strain was elliptical spherical or spherical, and that cells of different sizes were mixed.

2. Phylogenetic analysis based on 18S ribosomal DNA sequence Using DNA extracted from cells of AB-1C strain as a template, 18S ribosomal DNA was amplified by PCR and sequence analysis was performed. The base sequence of 18S ribosome DNA of AB-1C strain is shown in SEQ ID NO: 1.
The sequence of the primer used for the amplification of 18S ribosomal DNA is as follows.
Forward primer: 5'-TACCTGGTTGATCCTGCCAG-3' (SEQ ID NO: 2)
Reverse primer: 5'-CCTTCCGCAGGTTCACCTAC-3'(SEQ ID NO: 3)

Based on the base sequence of 18S ribosomal DNA of AB-1C strain, molecular phylogenetic analysis by maximum likelihood method was performed. Specifically, the sequences of closely related species obtained from the database were aligned using MEGA5.05 software (http://www.megasoftware.net/), and a molecular phylogenetic tree was prepared by the neighborhood joining method. The molecular phylogenetic tree based on the base sequence of 18S ribosomal DNA is shown in FIG. The statistical support value of the internal branch in the molecular phylogenetic tree was obtained by the bootstrap method (repeated 1000 times).
From FIG. 2, it was found that the 18S ribosomal DNA of the AB-1C strain is closely related to the known algae species shown in Table 6 below.

From the results of the above molecular phylogenetic analysis, it was determined that the AB-1C strain belongs to the genus Scenedesmus.

3. 3. Component analysis of AB-1C strain (1) Culture of AB-1C strain AB-1C strain was aerated and cultured using SGI medium. The culture temperature was 25 ° C., and the cells were shake-cultured for 4 to 7 days under a white fluorescent lamp (4000 to 4500 lux) while aerating. The cultured AB-1C strain was collected by centrifugation and lyophilized.

(2) Separation and analysis of useful substances (2-1) Separation and analysis of carotenoids The content of carotenoids was analyzed by requesting the Japan Food Research Laboratories. The specific analysis method is as shown below.

Separation and analysis of astaxanthin 0.1-0.2 g of freeze-dried AB-1C strain was weighed. Next, using the weighed AB-1C strain, the ester-type astaxanthin was enzymatically decomposed according to the following flow, and then separated and purified to prepare a sample to be used for high performance fluid chromatography. The analysis conditions for high performance liquid chromatography are shown below.

(Astaxanthin separation and purification process)
Sampling (0.2 g, in a mortar)
↓ + sea sand (appropriate amount)
↓ + water (1 mL)
↓ Mixed solution of chloroform and methanol (chloroform: methanol = 2: 1)
Grinding extraction (grinding and extracting in several batches. Furthermore, suction filtration with an 11G glass filter)
↓ Extract is a mixed solution of chloroform and methanol (chloroform: methanol = 2: 1)
Distill the constant volume solvent to 100 mL with ↓ ↓ + Acetone (separate 10 mL of constant volume solution into a 50 mL centrifuge tube)
↓ + 0.05M Tris buffer (pH 7.0) (6 mL)
↓ + Cholesterol esterase (1800 μL) (60 units / mL)
Enzyme reaction (37 ° C, 120 minutes)
↓ + Petroleum ether (10mL)
Dissolution ↓
Centrifugation (1500 rpm, 5 minutes)
↓ Collect the upper layer in a 100 mL eggplant flask. Repeat the above centrifugation twice more from the above enzymatic reaction. ↓
Solvent distillation ↓ + mobile phase (addition of predetermined amount)
High performance liquid chromatography

(Analysis conditions for high performance liquid chromatography)
High Performance Liquid Chromatograph: LC-20AT, Shimadzu Corporation Detector: Ultraviolet Visible Absorptiometer, SPD-20A, Shimadzu Corporation Column: Luna 3μ Sirica Φ4.6mm × 150mm, Phenomenex Column Temperature: 30 ℃
Mobile phase: Mixed solution of hexane and acetone (hexane: acetone = 82:18)
Flow rate: 1.2 mL / min
Measurement wavelength: 470 nm
Injection volume: 40 μL

Separation and analysis of canthaxanthin 1 g of lyophilized AB-1C strain was weighed. Next, the weighed AB-1C strain was separated and purified according to the following flow to prepare a sample to be used for high performance fluid chromatography. The analysis conditions for high performance liquid chromatography are shown below.

(Canthaxanthin separation and purification process)
Sampling (1g, in a mortar)
↓ + sea sand (appropriate amount)
↓ + water (1 mL)
↓ Mixed solution of chloroform and methanol (chloroform: methanol = 2: 1)
Grinding extraction (grinding and extracting in several batches. Furthermore, suction filtration with an 11G glass filter)
↓ Extract is a mixed solution of chloroform and methanol (chloroform: methanol = 2: 1)
↓ Constant volume to 100 mL ↓ Volume 60 mL in a 100 mL eggplant flask Distill off the solvent ↓ + HAET mixed solution (30 mL)
↓ (Mixed solution of hexane, acetone, ethanol and toluene)
↓ (Hexane: Acetone: Ethanol: Toluene = 10: 7: 6: 7)
↓ + water (1 mL)
↓ 40w / v% potassium hydroxide-methanol solution (4mL)
Saponification (30 minutes in a 56 ° C water bath)
↓ Transfer to a 300 mL brown separatory funnel ↓ + HAET mixed solution (30 mL)
↓ + 3w / v% anhydrous sodium sulfate solution (40mL)
↓ + HAET mixed solution (30 mL)
Extraction ↓ Wash several times with water (50 mL) and collect upper layer ↓
Solvent distillation ↓ + 2-propanol (added in a predetermined amount)
High performance liquid chromatography

(Analysis conditions for high performance liquid chromatography)
High Performance Liquid Chromatograph: LC-20AT, Shimadzu Corporation Detector: Ultraviolet Visible Absorptiometer, SPD-20A, Shimadzu Corporation Column: Cadenza CL-C18 Φ4.6mm × 250mm, Intact Co., Ltd. Column Temperature : 40 ° C
Mobile phase: Mixed solution of methanol and water (methanol: water = 96: 4)
Flow rate: 1.0 mL / min
Measurement wavelength: 470 nm
Injection volume: 20 μL

Separation and analysis of lutein 0.1-0.2 g of freeze-dried AB-1C strain was weighed. Next, using the weighed AB-1C strain, the ester-type lutein was saponified according to the following flow, and then separated and purified to obtain a sample to be used for high performance fluid chromatography. The analysis conditions for high performance liquid chromatography are shown below.

(Lutein separation and purification process)
Sampling (0.1-0.2 g, in a mortar)
↓ + sea sand (appropriate amount)
↓ + water (1 mL)
↓ Mixed solution of chloroform and methanol (chloroform: methanol = 2: 1)
Grinding extraction (grinding and extracting in several batches. Furthermore, suction filtration with an 11G glass filter)
↓ Extract is a mixed solution of chloroform and methanol (chloroform: methanol = 2: 1)
↓ Constant volume to 100 mL ↓ Volume 2 mL in a 100 mL eggplant flask Distill off the solvent ↓ + HAET mixed solution (30 mL)
↓ + water (1 mL)
↓ 40w / v% potassium hydroxide-methanol solution (4mL)
Saponification (30 minutes in a 56 ° C water bath)
↓ Transfer to a 300 mL brown separatory funnel ↓ + HAET mixed solution (30 mL)
↓ + 3w / v% anhydrous sodium sulfate solution (40mL)
↓ + HAET mixed solution (30 mL)
Extraction ↓ Wash several times with water (50 mL) and collect upper layer ↓
Solvent distilling ↓ + mixed solution of hexane and acetone (added in a predetermined amount) (hexane: acetone = 81: 19)
High performance liquid chromatography

(Analysis conditions for high performance liquid chromatography)
High Performance Liquid Chromatograph: LC-10AT _VP , Shimadzu Corporation Detector: Ultraviolet Visible Absorptiometer, SPD-20A, Shimadzu Corporation Column: Luna 3μ Silica Φ4.6mm × 150mm, Phenomenex Column Temperature: 30 ℃
Mobile phase: Mixed solution of hexane and acetone (hexane: acetone = 82:18)
Flow rate: 1.2 mL / min
Measurement wavelength: 450 nm
Injection volume: 30 μL

Separation and analysis of β-carotene Weighed 0.1-0.2 g of freeze-dried AB-1C strain. Next, the weighed AB-1C strain was separated and purified according to the following flow to prepare a sample to be used for high performance fluid chromatography. The analysis conditions for high performance liquid chromatography are shown below.

(Β-carotene separation and purification process)
Sampling (0.1-0.2 g, in a mortar)
↓ + sea sand (appropriate amount)
↓ + water (1 mL)
↓ Mixed solution of chloroform and methanol (chloroform: methanol = 2: 1)
Grinding extraction (grinding and extracting in several batches. Furthermore, suction filtration with an 11G glass filter)
↓ Extract is a mixed solution of chloroform and methanol (chloroform: methanol = 2: 1)
↓ Constant volume to 100 mL ↓ Volume 2 mL in a 100 mL eggplant flask Distill off the solvent ↓ + HAET mixed solution (30 mL)
↓ + water (1 mL)
↓ 40w / v% potassium hydroxide-methanol solution (4mL)
Saponification (30 minutes in a 56 ° C water bath)
↓ Transfer to a 300 mL brown separatory funnel ↓ + HAET mixed solution (30 mL)
↓ + 3w / v% anhydrous sodium sulfate solution (40mL)
↓ + HAET mixed solution (30 mL)
Extraction ↓ Wash several times with water (50 mL) and collect upper layer ↓
Solvent distilling ↓ + mixed solution of hexane and acetone (added in a predetermined amount) (hexane: acetone = 81: 19)
↓ Distill 2 mL of solvent in a 50 mL eggplant flask ↓ + Ethanol (add a predetermined amount)
High performance liquid chromatography

(Analysis conditions for high performance liquid chromatography)
High Performance Liquid Chromatograph: LC-20AD, Shimadzu Corporation Detector: Ultraviolet Visible Absorptiometer, SPD-20AV, Shimadzu Corporation Column: Inertsil ODS-4.5 μm Φ4.6 mm × 250 mm, GL Sciences Co., Ltd. Column temperature: 40 ° C
Mobile phase: A mixed solution of acetonitrile, methanol, tetrahydrofuran and acetic acid (acetonitrile: methanol: tetrahydrofuran: acetic acid = 55: 40: 5: 0.1) (containing 0.05 g / L dl-α-tocopherol)
Flow rate: 1.5 mL / min
Measurement wavelength: 455 nm
Injection volume: 20 μL

-Analysis of total carotenoids The content of total carotenoids was measured by absorptiometry. Among the carotenoids confirmed to be contained, astaxanthin, which is the component with the highest content, was quantified under the measurement conditions. It was calculated from the absorption coefficient of astaxanthin E ^1% _{1 cm} = 2200 (wavelength 478 nm, acetone).

As a result of the measurement, the total carotenoid content in 100 g of the dry weight of the AB-1C strain was 666 mg, the astaxanthin content was 415 mg, the canthaxanthin content was 66.9 mg, and β-carotene. The content of was 42.3 mg and the content of lutein was 34.4 mg. The results are also shown in Table 7 below. As a control, hematococcus and Monoradium sp. The content of astaxanthin in 100 g of the dry weight of the GK-12 strain is shown.

(2-2) Separation and analysis of fatty acids 1 to 30 mg of freeze-dried AB-1C strain was weighed. Next, the AB-1C strain was placed in a pressure resistant test tube with a cap. By adding 0.4 mL of BF ₃ -Methanol solution and reacting at 100 ° C. for 40 minutes, the neutral fatty acid contained in the cells of the AB-1C strain was converted to fatty acid methyl (biodiesel fuel component). .. After completion of the reaction, the mixture was cooled and the pentane was put into the reaction solution to transfer the produced fatty acid methyl to the pentane layer. After leaving for a certain period of time, the pentane layer was collected. This pentane extraction was repeated several times. Then, the obtained pentane extract was volatilized and recovered with the fatty acid methyl as a residue. This residue was dissolved in hexane to prepare a sample for fatty acid analysis.

Next, the fatty acid content in the sample was measured by capillary gas chromatography (GC, GC-2010, manufactured by Shimadzu Corporation). The analysis conditions for capillary gas chromatography are shown below.

(Analysis conditions for capillary gas chromatography)
Column: InertCAP WAX (manufactured by GL Science, 30m, inner diameter 0.25mm)
Gas: Helium gas (He, 70 kPa)
Detection: FID

As a result of the measurement, the content of EPA in 100 g of the dry weight of the AB-1C strain was 400 mg.
In addition, the content of oleic acid in the dry weight of AB-1C strain was 17.8% by mass, the content of palmitic acid was 5.1% by mass, the content of linoleic acid was 4.6% by mass, and α-linolenic acid. The acid content was 4.5% by mass and the stearic acid content was 1.8% by mass.
The content of oleic acid in the fatty acid is 44.6% by mass, the content of palmitic acid is 12.8% by mass, the content of linoleic acid is 11.5% by mass, and the content of α-linolenic acid is 11. The content of 0.4% by mass, the content of stearic acid was 4.6% by mass, the content of γ-linolenic acid was 0.2% by mass, and the content of EPA was 0.9% by mass.
Some of the above results are also shown in Table 7 below. As a control, Monorapidium sp. The content of EPA in 100 g of the dry weight of the GK-12 strain is shown.

(2-3) Separation and analysis of hydrocarbons 1 to 30 mg of freeze-dried AB-1C strain was weighed. Next, the AB-1C strain was placed in an assist tube containing beads. After putting 0.1M of a methanol solution of 0.1M potassium hydroxide into it, it was put into a test tube with a cap. Next, it was saponified by reacting at 80 ° C. for 90 minutes. After completion of the reaction, the mixture was cooled, 1.0 mL of water was added to the reaction solution and stirred, and 2 mL of hexane was added and stirred. Next, it was allowed to stand and the hexane layer was collected in a test tube with a cap. Next, the hexane was vaporized in the dryer with the cap open. Next, 50 μL of a trimethylsilyl (TMS) agent was added, capped, and then left at 60 ° C. for 30 to 60 minutes. Next, 0.5 mL of water was added and mixed, and then 0.5 mL of hexane was added. The hexane layer was then transferred to a 1.5 mL vial to vaporize the hexane. Accurately 1 mL of hexane was added thereto to prepare a sample for hydrocarbon analysis.

Next, the content of hydrocarbons in the sample was measured by capillary gas chromatography (GC, GC-2010, manufactured by Shimadzu Corporation). The analysis conditions for capillary gas chromatography are shown below.

(Analysis conditions for capillary gas chromatography)
Column: DB-1ms (SEG, inner diameter 0.25mm)
Gas: Helium gas (He, 70 kPa)
Detection: FID

As a result of the measurement, the content of hydrocarbon in the oil component was 66.8% by mass.

From the above, from the Senedesmus SP AB-1C strain, a large number of useful substances such as hydrocarbons that can be fuel oils, functional oils such as linoleic acid, α-linolenic acid, and EPA, and carotenoids such as astaxanthin are available. It was confirmed that it could be obtained.

According to the Senedesmus SP AB-1C strain, useful substances such as biofuels, functional oils or functional substances can be obtained. Further, the obtained useful substance can be used for foods such as functional foods or nutritional supplements, pet foods for pet animals, feeds for livestock and farmed fish and shellfish, cosmetics and the like.

Claims (13)

Scenedesmus sp. AB-1C strain (FERM P-22338). The Senedesmus SP AB-1C strain according to claim 1 and an extract thereof are contained.
A nutritional supplement containing the scenedesmus SP AB-1C strain and its extract containing eicosapentaenoic acid as a nutritional component. The nutritional supplement according to claim 2, wherein the Senedesmus SP AB-1C strain and an extract thereof contain astaxanthin as a nutritional component. The nutritional supplement according to claim 3, wherein the content of astaxanthin is 300 mg or more and 600 mg or less per 100 g of the dry weight of the Senedesmus SP AB-1C strain. A nutritional component supplementing composition comprising the nutritional supplement according to any one of claims 2 to 4. The nutritional component supplement composition according to claim 5, further comprising at least one selected from the group consisting of carotenoids other than astaxanthin, linoleic acid and α-linolenic acid as the nutritional component. The nutritional component supplement composition according to claim 5 or 6, which is a food, a functional food, or a dietary supplement. The nutritional component supplement composition according to claim 5 or 6, which is a feed or pet food. The nutritional component supplement composition according to claim 5 or 6, which is a cosmetic product. A method for producing a nutritional component, comprising a culture step for culturing the Senedesmus sp. AB-1C strain according to claim 1. Further, after the culture step,
A crushing step of crushing the Senedesmus SP AB-1C strain to obtain a cell crushed product, and
The separation step of separating the nutrient component from the cell crushed product, and
10. The method for producing a nutritional component according to claim 10. The method for producing a nutritional component according to claim 10 or 11, wherein the nutritional component is one or more selected from the group consisting of eicosapentaenoic acid, carotenoids, linoleic acid and α-linolenic acid. The method for producing a nutritional component according to claim 12, wherein the carotenoids are astaxanthin.

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