JP7034465B2 - Method for producing crayfish with changed body color and feed for producing crayfish with changed body color - Google Patents
Method for producing crayfish with changed body color and feed for producing crayfish with changed body color Download PDFInfo
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Description
本発明は、自然界には存在しない蛍光様の色彩を有するアメリカザリガニの体色を作出し、かつ、その体色を長期にわたり維持する方法及び飼料に関する。 The present invention relates to a method and a feed for producing a body color of a crayfish having a fluorescent-like color that does not exist in nature and maintaining the body color for a long period of time.
アメリカザリガニは本来アメリカ原産の甲殻類であるが、世界各地の淡水系に広く分布するようになり、日本でも古くから水辺に生息する身近な水棲動物として知られている。体長は８～１２ｃｍほどで、若い個体は薄茶色を呈しているが、脱皮を繰り返して成長すると赤色又は赤褐色に変化する。 The crayfish is originally a crustacean native to the United States, but it has become widely distributed in freshwater systems around the world, and has long been known as a familiar aquatic animal that inhabits the waterside in Japan. The body length is about 8 to 12 cm, and the young individual has a light brown color, but when it grows by repeated molting, it turns red or reddish brown.
近年、アメリカザリガニは突然変異又は人為的に体色を変色させた個体が観賞用としてペットショップ等で販売されており、その色彩は、突然変異で体色が変色したアメリカザリガニとしては、白色、青色、橙色、さくら色、スーパーレッドと呼ばれる赤色などが知られている。人為的に体色を変化させる場合は、天然の赤いザリガニにアジやサバの切り身などのカロテノイド含有量の少ない餌を与えることで、赤から青色、白色へ体色を変化させることができる。また、白色のザリガニにクチナシの色素を与えることで黄色く変色させるなどの知見が得られている。 In recent years, the American crayfish has been sold at pet shops for ornamental purposes by individuals whose body color has been mutated or artificially discolored. Blue, orange, cherry, and red called super red are known. When artificially changing the body color, the body color can be changed from red to blue or white by feeding a natural red crayfish with a diet containing a low carotenoid content such as horse mackerel or mackerel fillet. In addition, it has been found that white crayfish are discolored yellow by giving gardenia pigment.
例えば、特開平７－１７９３８８号公報には、甲殻類の飼料中に、１種又はそれ以上のカロテノイド類を含有させたカロテノイド強化飼料を与えることにより、ロブスター等の甲殻類の甲皮を着色することが記載されている（特許文献１）。 For example, Japanese Patent Application Laid-Open No. 7-179388 colors the crustacean shell of lobster and the like by feeding a carotenoid-enriched feed containing one or more carotenoids in the crustacean feed. (Patent Document 1).
また、特開２００５－１９８５７５号公報には、ベース飼料にカロチノイド色素飼料を含浸させた専用飼料を調製しザリガニに摂取させることにより、金色（山吹色）の体色を有するザリガニを作り出す方法が開示されている（特許文献２）。 Further, Japanese Patent Application Laid-Open No. 2005-198575 discloses a method for producing a crayfish having a golden (Yamabuki color) body color by preparing a special feed in which a base feed is impregnated with a carotinoid pigment feed and feeding the crayfish. (Patent Document 2).
色彩のバリエーションには上記のように白色、青色、黄色、橙色、さくら色、緑色、褐色又は赤色などが知られているが、これまで蛍光様の色彩を有するアメリカザリガニを作出した例は、人為的に作出した個体においても、突然変異個体においても知られていない。また、アメリカザリガニは生きている限り定期的に脱皮を行う。そのため従来の方法では、ザリガニが脱皮するたびに着色した色素が甲皮とともに脱落し、元の個体の色（白色）に戻ってしまうという問題があった。 As mentioned above, white, blue, yellow, orange, cherry, green, brown, or red are known as color variations, but the examples of producing American crabs with fluorescent-like colors have been artificial. It is not known in the individual produced or in the mutant individual. In addition, the crayfish molts regularly as long as it is alive. Therefore, in the conventional method, there is a problem that the colored pigment is shed together with the instep every time the crayfish molts and returns to the original color (white) of the individual.
従って本発明の主な目的は、自然界には存在しない蛍光様の色彩を有するアメリカザリガニを作出する技術を提供することにある。また、本発明の他の目的は、蛍光様の色彩を有するアメリカザリガニの体色を長期にわたり維持する技術を提供することにある。 Therefore, a main object of the present invention is to provide a technique for producing a crayfish having a fluorescent-like color that does not exist in nature. Another object of the present invention is to provide a technique for maintaining the body color of a crayfish having a fluorescent-like color for a long period of time.
本発明者は上記課題を解決するため種々の色素を白色のアメリカザリガニの餌に混合し、給餌後や脱皮後の体色変化を観察したところ、アナトー色素及びパプリカ色素を与えたザリガニがこれまでに知られていない蛍光色様の発色を呈し、脱皮後もその効果が維持されるとの知見を得た。 In order to solve the above problems, the present inventor mixed various pigments with the white crayfish diet and observed the change in body color after feeding and molting. It was found that it exhibits a fluorescent color-like color that is not known to the crayfish, and its effect is maintained even after molting.
本発明は係る知見に基づきなされたものであり、ザリガニ用の餌にカロテノイド色素を含有させたカロテノイド含有飼料を調製する工程と、カロテノイド含有飼料を白色の色彩を有するアメリカザリガニに給餌して該アメリカザリガニの体色を蛍光様の色彩に変化させる工程と、を有する、蛍光様の色彩を有するアメリカザリガニの作出方法を提供するものである。 The present invention has been made based on the above findings, and is a step of preparing a carotenoid-containing feed containing a carotenoid pigment in a crayfish feed, and feeding the carotenoid-containing feed to a white-colored crayfish. It provides a method for producing a crayfish having a fluorescent-like color, which comprises a step of changing the body color of the crayfish to a fluorescent-like color.
本発明の蛍光様の色彩を有するアメリカザリガニの作出方法及び蛍光様の色彩を有するザリガニ作出用の飼料によれば、自然界には存在しない蛍光様の色彩を有するアメリカザリガニを作出し、かつ、その体色を長期にわたり維持する技術を提供することができる。 According to the method for producing crayfish having a fluorescent-like color and the feed for producing crayfish having a fluorescent-like color of the present invention, a crayfish having a fluorescent-like color that does not exist in nature is produced, and the crayfish is produced. It is possible to provide a technique for maintaining body color for a long period of time.
本実施形態に係る蛍光様の色彩を有するアメリカザリガニの作出方法は、ザリガニ用の餌にカロテノイド色素を含有させたカロテノイド含有飼料を調製する工程と、カロテノイド含有飼料を白色の色彩を有するアメリカザリガニに給餌して該アメリカザリガニの体色を蛍光様の色彩に変化させる工程と、を有する。 The method for producing a crayfish having a fluorescent-like color according to the present embodiment is a step of preparing a carotenoid-containing feed containing a carotenoid pigment in a crayfish feed, and a method for preparing a carotenoid-containing feed into a white-colored crayfish. It has a step of feeding and changing the body color of the crayfish to a fluorescent-like color.
本実施形態において蛍光様の色彩とは、一般的な意味の蛍光、すなわち、光（紫外・可視光）のエネルギーを吸収し発光するフォトルミネッセンスとは異なるが、あたかも蛍光発色しているかのように見える色彩をいう。 In the present embodiment, fluorescence-like color is different from fluorescence in a general sense, that is, photoluminescence that absorbs and emits light (ultraviolet / visible light) energy, but as if fluorescent color is developed. The color that can be seen.
カロテノイド（carotenoid）色素は、黄、橙、赤色などを示す天然色素の一群をいう。微生物、動物、植物などからこれまで750種類以上のカロテノイド色素が同定されている。例えば、アナトー色素、イモカロテン色素、エビ色素、オキアミ色素、オレンジ色素、カニ色素、パプリカ（トウガラシ）色素、トウモロコシ色素、トマト色素、パーム油カロテン色素、ファフィア色素、ヘマトコッカス色素、マリーゴールド色素などを挙げることができる。但し、カロテノイド色素のすべてが本実施形態に利用できるわけではなく、例えば、βカロテン色素は遺伝的に固定された白色ザリガニの体色変化に影響を与えないため、本発明の効果が得られない。 Carotenoid pigments are a group of natural pigments that show yellow, orange, red, and the like. More than 750 types of carotenoid pigments have been identified so far from microorganisms, animals, plants, etc. For example, annatto pigment, imokarotene pigment, shrimp pigment, krill pigment, orange pigment, crab pigment, paprika (capsicum) pigment, corn pigment, tomato pigment, palm oil carotene pigment, fafia pigment, hematococcus pigment, marigold pigment and the like. be able to. However, not all carotenoid pigments can be used in the present embodiment, and for example, β-carotene pigments do not affect the body color change of genetically fixed white crayfish, so that the effect of the present invention cannot be obtained. ..
本実施形態に係る蛍光様の色彩を有するザリガニ作出用の飼料における前記カロテノイド色素の含有量は色素の種類に応じて任意に設定することができるが、少なすぎる場合はザリガニの発色が弱くなる一方、多すぎる場合は最悪の場合色素が体液循環の管に詰まるなどの原因により固体が死亡してしまうため、カロテノイド含有飼料の総重量に対して１０～６０重量％であることが好ましい。 The content of the carotenoid pigment in the feed for producing crayfish having a fluorescent-like color according to the present embodiment can be arbitrarily set according to the type of the pigment, but if it is too small, the color development of the crayfish will be weakened. If the amount is too large, the solid will die due to the dye clogging the tube of the body fluid circulation in the worst case, so that the amount is preferably 10 to 60% by weight based on the total weight of the carotenoid-containing feed.
本実施形態においては、鮮やかな蛍光様の色彩をザリガニに発色させる観点からは、前記カロテノイド色素がアナトー色素又はパプリカ色素であることが好ましい。 In the present embodiment, the carotenoid dye is preferably an annatto dye or paprika dye from the viewpoint of causing the crayfish to develop a vivid fluorescent color.
アナトー（annatto）色素は、ベニノキ科ベニノキの種子から抽出した色素をいい、ビキシン（Bixin）、ノルビキシン（Norbixin）が主成分で、色素単独では、酸性で黄色、中性で赤色を呈する。一般的には、黄色～赤色の食品用（食品添加物）または化粧品用（口紅など）色素として知られている。食品用には既存添加物のアナトー（アナトー色素）と、アルカリ加水分解抽出した指定添加物の水溶性アナトー（ナトリウム塩・カリウム塩）に分けられるが、本実施形態においてはいずれの種類のアナトー色素も用いることができる。 Annatto pigment refers to a pigment extracted from the seeds of Bixaceae Achiote, which is mainly composed of bixin and norbixin, and the pigment alone exhibits an acidic yellow color and a neutral red color. Generally, it is known as a yellow to red food dye (food additive) or cosmetic (lipstick, etc.) pigment. For food products, it is divided into annatto (annatto pigment), which is an existing additive, and water-soluble annatto (sodium salt / potassium salt), which is a designated additive extracted by alkaline hydrolysis. Can also be used.
アナトー色素を含有する餌を給餌することにより、白色ザリガニの体色を蛍光オレンジ様の色彩に変化させることができる。体色の変化はアナトー色素含有飼料を与えて１日～３日後には確認することができる。 By feeding a bait containing annatto pigment, the body color of the white crayfish can be changed to a fluorescent orange-like color. Changes in body color can be confirmed 1 to 3 days after feeding the annatto pigment-containing feed.
本実施形態に用いるパプリカ色素は、ナス科トウガラシ（Capsicum annuum LINNE）の果実より抽出して得られた色素をいい、色素単独では、橙～赤色を呈する。 The paprika pigment used in the present embodiment refers to a pigment obtained by extracting from the fruit of Capsicum annuum LINNE of the Solanaceae family, and the pigment alone exhibits an orange to red color.
パプリカ色素を含有する餌を給餌することにより、白色ザリガニの体色を蛍光ピンク様の色彩に変化させることができる。体色の変化はパプリカ色素含有飼料を与えてから約３週間後に確認することができる。 By feeding a bait containing a paprika pigment, the body color of the white crayfish can be changed to a fluorescent pink-like color. Changes in body color can be confirmed about 3 weeks after feeding the paprika pigment-containing feed.
上記カロテノイド色素と混合されるザリガニ用の餌の種類は特に限定はなく、市販のザリガニ飼育用に調製された餌を使用することができる。 The type of food for crayfish mixed with the carotenoid pigment is not particularly limited, and a commercially available food prepared for crayfish breeding can be used.
本実施形態においては、所望の色彩を付与するアメリカザリガニとして白色のアメリカザリガニを利用する。白色のアメリカザリガニとは、アメリカザリガニの突然変異個体で、全身又は体の大部分が遺伝的に白色であるアメリカザリガニをいう（以下、単に「白ザリガニ」ともいう）。但し、一般的なアメリカザリガニにカロテノイド含有量が少ないエサ（アジやサバの切り身など）を与えて人為的に体色を白くしたものは、餌にカロテノイドが含まれており、青や赤色と混ざり蛍光様の色彩が見られないため、対象から除かれる。 In the present embodiment, a white crayfish is used as a crayfish that imparts a desired color. The white crayfish is a mutant crayfish of the crayfish, and is a crayfish whose whole body or most of the body is genetically white (hereinafter, also simply referred to as "white crayfish"). However, if a general American crayfish is given food with a low carotenoid content (such as horse mackerel or mackerel fillet) to artificially whiten its body color, the food contains carotenoids and is mixed with blue and red. Excluded from the subject because no fluorescent-like color is seen.
本実施形態においては、蛍光様の色彩を有するアメリカザリガニも、一般的なアメリカザリガニと同様、生きている限り定期的に脱皮を行う。これまで知られている、発色させたい色を有する色素をザリガニ飼料に混合して通常の餌と同様に摂取させることで人為的に体色を変化させたザリガニは、脱皮を行うことにより色素が脱皮した殻に移行し、脱皮後の個体は白ザリガニに戻ってしまうが、本実施形態の方法で着色したアメリカザリガニは脱皮によっては元の白ザリガニに戻ることなく、一部又は多くの色素が脱皮後の体に残存する。 In the present embodiment, the crayfish having a fluorescent-like color is also molted regularly as long as it is alive, like the general crayfish. Crayfish that have been known so far and have artificially changed their body color by mixing a pigment with a color that they want to develop with a crayfish feed and ingesting it in the same way as a normal diet, the pigment is removed by molting. The individual crayfish after molting shifts to the dehulled shell and returns to the white crayfish, but the crayfish colored by the method of this embodiment does not return to the original white crayfish depending on the molting, and some or many pigments are present. It remains on the body after molting.
脱皮後の個体に継続してカロテノイド含有飼料を与えることにより、蛍光様色素をより濃く発色させることができる。一方、脱皮後の個体にカロテノイドを含まない一般の飼料を与えると、直ちに退色することはないが、脱皮を行うごとに元の白ザリガニの色に体色が戻っていく。 By continuously feeding the carotenoid-containing feed to the individual after molting, the fluorescent-like pigment can be developed in a deeper color. On the other hand, when a general feed containing no carotenoid is given to an individual after molting, the color does not fade immediately, but the body color returns to the original color of the white crayfish every time the molting is performed.
本実施形態に係る蛍光様の色彩を有するアメリカザリガニの飼育方法は、餌以外については、一般的なアメリカザリガニの飼育方法に準じて行うことができる。本実施形態に係る蛍光様の色彩を有するザリガニ作出用の飼料の投与量は特に限定されないが、例えば、楕円状（縦約２ｍｍ、横約１ｍｍ）に調製したカロテノイド含有飼料を１日２～３粒給餌することで、アナトー色素含有飼料では１日後、パプリカ色素含有飼料では約３週間後に、所望の蛍光様色彩を有するアメリカザリガニを得ることができる。 The method for breeding crayfish having a fluorescent color according to the present embodiment can be carried out according to a general method for breeding crayfish except for food. The dose of the feed for producing crayfish having a fluorescent color according to the present embodiment is not particularly limited, but for example, a carotenoid-containing feed prepared in an elliptical shape (length: about 2 mm, width: about 1 mm) is prepared 2-3 times a day. By grain feeding, the crayfish having the desired fluorescence-like color can be obtained after 1 day with the anato pigment-containing feed and after about 3 weeks with the paprika pigment-containing feed.
１．アナトー色素含有飼料による体色変化
（１）方法
市販されているザリガニ用の餌（キョーリン株式会社の「Hikariザリガニのエサ」）2130 mgに対し、オレンジ色を呈するアナトー色素（和光純薬工業株式会社）870mgを添加したものを適量の水を加え乳鉢でよく練り合わせ、楕円状のアナトー色素含有飼料（縦約２ｍｍ、横約１ｍｍ）を調製した。また、比較例として、市販のザリガニ用の餌（キョーリン株式会社の「Hikariザリガニのエサ」）1086 mgに対し、βカロテン色素（東京化成工業株式会社製）10mgを添加したものを適量の水を加え乳鉢でよく練り合わせ、楕円状のβカロテン色素含有飼料（縦約２ｍｍ、横約１ｍｍ）を調製した。
1. 1. Body color change with annatto pigment-containing feed (1) Method Annatto pigment (Wako Pure Chemical Industries, Ltd.) that exhibits an orange color to 2130 mg of commercially available feed for annatto (Kyorin Co., Ltd.'s "Hikari crab food") ) 870 mg was added to an appropriate amount of water and kneaded well in a dairy pot to prepare an elliptical annatto pigment-containing feed (length: about 2 mm, width: about 1 mm). As a comparative example, add 10 mg of β-carotene pigment (manufactured by Tokyo Kasei Kogyo Co., Ltd.) to 1086 mg of commercially available feed for crayfish (Kyorin Co., Ltd.'s "Hikari crayfish food"), and add an appropriate amount of water. In addition, it was well kneaded in a mortar to prepare an elliptical β-carotene pigment-containing feed (length: about 2 mm, width: about 1 mm).
白ザリガニ個体としてペットショップ等で購入した体長約３cm、３匹の白ザリガニを使用した。白ザリガニ個体に上記のように調製した飼料を１日２～３粒給餌し、体色の変化を観察した。 Three white crayfish with a body length of about 3 cm purchased from a pet shop or the like were used as individual white crayfish. Two to three grains of the feed prepared as described above were fed to individual white crayfish, and changes in body color were observed.
（２）結果
結果を図１に示す（蛍光様色彩の発色の度合いを－又は＋で示した）。アナトー色素含有飼料を給餌したザリガニは、給餌後、１日後には色の変化が確認できるほど短期間ではっきりと体色変化が確認できた。アナトー色素含有飼料を与えた個体は、蛍光色に近いオレンジ色になった。実験開始から約２か月後、脱皮をすることでオレンジ色の脱皮殻が得られたが、体色は黄色味を帯びており、アナトー色素がザリガニに残存していることを確認した。その後、通常のザリガニ用の餌（キョーリン株式会社の「Hikariザリガニのエサ」）を１日２～３粒与えることで、２度目の脱皮ではほぼ白ザリガニの体色に戻った。
(2) Results The results are shown in FIG. 1 (the degree of fluorescence-like color development is indicated by-or +). The body color of the crayfish fed with the annatto pigment-containing feed was clearly confirmed in a short period of time so that the color change could be confirmed one day after feeding. Individuals fed the annatto pigment-containing feed turned orange, which was close to the fluorescent color. Approximately two months after the start of the experiment, an orange molting shell was obtained by molting, but the body color was yellowish, and it was confirmed that the annatto pigment remained in the crayfish. After that, by feeding 2 to 3 grains of normal crayfish food (Kyorin Co., Ltd.'s "Hikari crayfish food") a day, the body color of the white crayfish was almost restored by the second molting.
一方、βカロテン飼料を給餌したザリガニは、給餌後から脱皮するときまで体色に変化はなく、１か月間の給餌実験においては、体色の変化は見られなかった。 On the other hand, the crayfish fed with the β-carotene feed did not change in body color from the time of feeding to the time of molting, and no change in body color was observed in the feeding experiment for one month.
２．アナトー色素の濃度を変えた給餌による体色変化
（１）方法
ザリガニの餌（キョーリン株式会社の「Hikariザリガニのエサ」）1.25ｇに対し、アナトー色素（和光純薬工業株式会社）510mgを添加し、適量の水を加え乳鉢で練り合わせて楕円状のアナトー色素含有飼料（縦約２ｍｍ、横約１ｍｍ）を調製した。また、この色素含有飼料を濃度10倍の餌として、色素量を1/2倍（濃度5倍）、1/4倍（濃度2.5倍）、1/10倍（濃度1倍）としたものを同様に作成した。
2. 2. Body color change by feeding with different annatto pigment concentration (1) Method To 1.25 g of crayfish feed (Kyorin Co., Ltd.'s "Hikari crayfish food"), 510 mg of annatto pigment (Wako Pure Chemical Industries, Ltd.) was added. , An appropriate amount of water was added and kneaded in a dairy pot to prepare an elliptical annatto pigment-containing feed (length: about 2 mm, width: about 1 mm). In addition, this pigment-containing feed is used as a feed with a concentration of 10 times, and the amount of pigment is 1/2 times (concentration 5 times), 1/4 times (concentration 2.5 times), and 1/10 times (concentration 1 times). Created in the same way.
白ザリガニ個体としてペットショップ等で購入した体長約３cm、４匹を使用した。各白ザリガニ個体に上記のように調製したアナトー色素濃度の異なる餌を1日1～２粒給餌し、体色の変化を観察した。 As white crayfish individuals, 4 animals with a body length of about 3 cm purchased at a pet shop or the like were used. Each white crayfish individual was fed with 1 to 2 grains of annatto pigment concentration prepared as described above per day, and changes in body color were observed.
（２）結果
結果を図２に示す（蛍光様色彩の発色の度合いを－又は＋で示した）。実験開始後、３日後にはすべての個体ではっきりと体色に変化が見られ、濃度依存的に体色が蛍光オレンジ色に変化した。
(2) Results The results are shown in FIG. 2 (the degree of fluorescence-like color development is indicated by-or +). Three days after the start of the experiment, a clear change in body color was observed in all the individuals, and the body color changed to fluorescent orange in a concentration-dependent manner.
３．パプリカ色素含有飼料による体色変化
（１）方法
市販されているザリガニ用の餌（キョーリン株式会社の「Hikariザリガニのエサ」）1600 mgに対し、赤色を呈するパプリカCWS（ヤエガキ発酵技研株式会社）30滴（約1950mg）を添加したものを適量の水を加え乳鉢でよく練り合わせ、楕円状のアナトー色素含有飼料（縦約２ｍｍ、横約１ｍｍ）を調製した。
3. 3. Body color change with paprika pigment-containing feed (1) Method Paprika CWS (Yaegaki Fermentation Giken Co., Ltd.) 30 that shows a red color against 1600 mg of commercially available feed for crayfish (Kyorin Co., Ltd.'s "Hikari crayfish food") 30 An appropriate amount of water was added to the mixture to which drops (about 1950 mg) were added, and the mixture was kneaded well in a dairy pot to prepare an elliptical annatto pigment-containing feed (length: about 2 mm, width: about 1 mm).
白ザリガニ個体としてペットショップ等で購入した体長約３cm、２匹の白ザリガニを使用した。白ザリガニ個体に上記のように調製したパプリカ色素含有飼料を１日２～３粒給餌し、体色の変化を観察した。脱皮後は市販のノーマル餌（キョーリン株式会社の「Hikariザリガニのエサ」）を与えたものをＡ、引き続きパプリカ含有餌を与え続けたものをＢとして観察を行った。 Two white crayfish with a body length of about 3 cm purchased from a pet shop or the like were used as individual white crayfish. Two to three paprika pigment-containing feeds prepared as described above were fed to individual white crayfish a day, and changes in body color were observed. After molting, observation was carried out with A being fed with a commercially available normal diet (“Hikari crayfish feed” from Kyorin Co., Ltd.) and B being continuously fed with a paprika-containing diet.
（２）結果
結果を図３、図４に示す（蛍光様色彩の発色の度合いを－又は＋で示した）。Ａ、Ｂともに、給餌を始めてすぐに体色変化は見られなかったが、１週間後から若干肌色のようになり、２週間ほどでピンク色が確認できるようになった。３週間後には全身がはっきりとした蛍光色に近いピンク色になった。１度目の脱皮では若干体色が薄くなったが、体色はピンク色のままだった（図３）。脱皮殻は、アナトー色素を用いた時とは異なり、ピンク色の殻ではなく、白い脱皮殻だった。その後、パプリカ色素含有飼料に代えて市販のノーマル餌（キョーリン株式会社の「Hikariザリガニのエサ」）を与えても、脱皮毎に体色は薄いピンク色を保持し、徐々に色が薄くなる傾向が観察されるが、本来の白色には戻りにくいことが観察された。
(2) Results The results are shown in FIGS. 3 and 4 (the degree of color development of the fluorescence-like color is indicated by-or +). In both A and B, no change in body color was observed immediately after the start of feeding, but after 1 week, the skin color became slightly flesh-colored, and pink color could be confirmed in about 2 weeks. After 3 weeks, the whole body turned pink, which was close to a clear fluorescent color. The body color became slightly lighter in the first molting, but the body color remained pink (Fig. 3). The molting shell was a white molting shell, not a pink shell, unlike when the annatto pigment was used. After that, even if a commercially available normal feed (Kyorin Co., Ltd.'s "Hikari crayfish feed") is given instead of the paprika pigment-containing feed, the body color remains light pink with each molting, and the color tends to gradually fade. Was observed, but it was observed that it was difficult to return to the original white color.
また、脱皮をしてもパプリカ色素含有餌を与え続けている個体（Ｂ）は、蛍光色に近いピンク色の度合いが高くなることが観察された（図４）。
In addition, it was observed that the individual (B), which continued to feed the paprika pigment-containing diet even after molting, had a higher degree of pink color close to the fluorescent color (FIG. 4).
Claims (4)
カロテノイド含有飼料を白色のアメリカザリガニに給餌して該アメリカザリガニの体色を変化させる工程と、
を有する、体色が変化したアメリカザリガニの作出方法であって、
前記白色のアメリカザリガニが、全身又は体の大部分が遺伝的に白色であるアメリカザリガニであり、
前記カロテノイド色素が、アナトー色素又はパプリカ色素である、
アメリカザリガニの作出方法。 The process of preparing a carotenoid-containing feed containing a carotenoid pigment in the feed for crayfish, and
The process of feeding a white crayfish with a carotenoid-containing feed to change the body color of the crayfish,
It is a method of producing a crayfish whose body color has changed .
The white crayfish is a crayfish whose whole body or most of its body is genetically white.
The carotenoid pigment is an annatto pigment or a paprika pigment.
How to make a crayfish.
前記アメリカザリガニが、全身又は体の大部分が遺伝的に白色であるアメリカザリガニであり、
前記カロテノイド色素が、アナトー色素又はパプリカ色素である、
アメリカザリガニ作出用の飼料。 A feed for producing crayfish with a changed body color that contains carotenoid pigments.
The crayfish is a crayfish whose whole body or most of its body is genetically white.
The carotenoid pigment is an annatto pigment or a paprika pigment.
Feed for producing crayfish.
請求項３に記載のアメリカザリガニ作出用の飼料。 The annatto pigment or the paprika pigment is 10 to 60% by weight based on the total weight of the feed .
The feed for producing crayfish according to claim 3.
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| JP2005198575A (en) | 2004-01-16 | 2005-07-28 | Aqua City:Kk | Method for changing body color of crawfish, feedstuff to be fed and crawfish |
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| JP2014132898A (en) | 2012-12-14 | 2014-07-24 | Suntory Beverage & Food Ltd | Container-packed beverage containing fruit juice and/or vegetable juice |
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| JP2005198575A (en) | 2004-01-16 | 2005-07-28 | Aqua City:Kk | Method for changing body color of crawfish, feedstuff to be fed and crawfish |
| JP2008532492A (en) | 2005-02-11 | 2008-08-21 | カラマズー・ホールディングス・インコーポレイテッド | Capsicum varieties exhibiting excessive accumulation of zeaxanthin and products obtained therefrom |
| JP2014132898A (en) | 2012-12-14 | 2014-07-24 | Suntory Beverage & Food Ltd | Container-packed beverage containing fruit juice and/or vegetable juice |
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