JP7029279B2 - Gazette bag and shaking type incubator - Google Patents

Description

The present invention relates to a gusset bag and a shaking type incubator .

Patent Document 1 discloses a shaking-type incubator using a gusset bag.
Patent Document 2 discloses a bag-in-box using a gusset bag.
Patent Document 3 discloses a culture apparatus using a cylindrical inner bag for drums.
Patent Document 4 discloses an inner bag for a drum can having a spout on the top surface.

Japanese Unexamined Patent Publication No. 2016-136928 Japanese Unexamined Patent Publication No. 2001-253474 Japanese Unexamined Patent Publication No. 2007-228629 Japanese Unexamined Patent Publication No. 2005-298038

When used as a container for various purposes such as culture, transportation, and storage, the conventional gusset bag becomes a substantially rectangular parallelepiped shape as described in Patent Documents 1 and 2 when the contents are filled and inflated. For this reason, when the bag is made large, there is a problem that it is not easy to pour and mix the contents at the corners of a substantially rectangular parallelepiped.

As a substantially cylindrical bag, as described in Patent Documents 3 and 4, an inner bag for a drum can having a top surface and a bottom surface at the top and bottom is known. However, when the bag is made large, there is a problem that it is not easy to fold the top surface and the bottom surface.
Further, with the conventional gusset bag and inner bag for drums, it may be difficult to manufacture a large bag with the existing film width.

The present invention has been made in view of the above circumstances, and provides a gusset bag and a shaking type culture device that can be easily folded even if they are large and can be made substantially cylindrical when inflated. Is the subject.

In order to solve the above problems, the present invention relates to the front and rear facing front and rear walls, and between one side end of the front wall in the width direction and one side end of the rear wall in the width direction. The width of the first side wall, the other side end in the width direction of the front wall, and the width of the rear wall. A second side wall that connects to the other side end in the direction and constitutes a gusset that is folded inward in the width direction at at least one point in between, the front wall and the said. The rear wall has a hypotenuse seal portion sealed along a hypotenuse portion whose width becomes narrower toward both upper and lower ends, and the number of locations where the gusset of the first side wall is folded inward in the width direction. Is G1, the number of points where the gusset of the second side wall is folded inward in the width direction is G2, and the number obtained by adding the numbers of the front wall and the rear wall is N = G1 + G2 + 2. The angle θ formed by the hypotenuse seal portions corresponding to both sides in the direction is 80 ° or less, at least one of the G1 or the G2 is 2 or more, the N is 5 or more, and the θ and the N are The product Nθ is in the range of 280 to 400 °, and the top surface formed by the upper ends of the upper and lower ends faces the front wall or the rear wall in the width direction. A first spout is provided between the hypotenuse seal portions, a tube is attached to the first spout, and the bottom surface formed by the lower ends of the upper and lower ends is the front wall or the said. In any of the rear walls, a second spout is provided between the hypotenuse seals facing the width direction, and a tube is attached to the second spout, and the upper end and the lower end are attached. Each of the above provides a gusset bag having a tip seal portion in which the inner surface of the front wall and the inner surface of the rear wall are directly opposed to each other and joined between the hypotenuse seal portions .

Of the first side wall or the second side wall, the side wall having two or more points folded inward in the width direction is composed of side films having different gusset portions. Each of the side films has a fold line at a portion to be folded inward in the width direction, and both side ends via the fold line are sequentially adjacent to each other between the front wall and the rear wall. It may be bonded to the side end portion of the film by a seal between the inner surfaces of each film.

The first side wall and the second side wall may have the same number of points folded inward in the width direction.
The N may be 5 to 10 .

The gusset bag may be a gusset bag for manufacturing or storing any of cultured cells, biopharmacy, regenerative medicine and other products.
The present invention also provides a shake-type culture apparatus including the gusset bag and an outer shell container for accommodating the gusset bag.

Since the gusset bag of the present invention has at least two or more gusset portions on at least one of the side walls, it is easy to fold even if it is large, and it can be made substantially cylindrical when inflated. Further, when each of the gusset portions is composed of different side films, it is possible to easily manufacture a bag having a size that cannot be manufactured with the existing film width.

It is a front view which shows an example of the state which the gusset bag is folded. It is a perspective view which shows an example of the state in which the gusset bag is inflated. FIG. 3 is a cross-sectional view taken along the line III-III of FIG. It is a cross-sectional view along the IV-IV line of FIG. It is a partially enlarged view which shows the upper and lower ends of a gusset bag. It is a perspective view which shows an example of the outer shell container which accommodates a culture container. It is a perspective view which shows an example of a culture apparatus.

Hereinafter, the present invention will be described with reference to the drawings based on the preferred embodiments.
FIG. 1 shows an example of a state in which the gusset bag of the present embodiment is folded. Further, FIG. 2 shows an example of a state in which the gusset bag of the present embodiment is inflated. The gusset bag 10 of the present embodiment includes a front wall 12 and a rear wall 13 facing each other in the front-rear direction, and side walls 18 and 19 connecting the front wall 12 and the rear wall 13. The left and right side walls 18 and 19 each include a front side film 14 and a rear side film 15.

The front wall 12 and the front side film 14, the front and rear side films 14, 15, and the rear wall 13 and the rear side film 15 are joined by a sealing portion 11, respectively. Further, the front wall 12 and the rear wall 13 have a hypotenuse seal portion 11b sealed along a hypotenuse portion whose width becomes narrower toward both upper and lower ends. The hypotenuse seal portion 11b is also included in the seal portion 11 between the side films 14 and 15. Between the upper and lower hypotenuse seal portions 11b, the side end portions of the film are joined by the side end seal portions 11a.

The front wall 12, the rear wall 13, and the side films 14 and 15 may have the spout 16, and the front wall 12 and the rear wall 13 may have the spout 16. It is preferable to do so. When the spout 16 is provided on either the front wall 12 or the rear wall 13, the spout 16 is provided between the left and right hypotenuse seal portions 11b and 11b facing each other in the width direction at at least one of the upper and lower ends. It is preferable to have. A tube 17 may be attached to the spout 16.
As shown in FIG. 1, the gusset bag of the present embodiment is vertically long as compared with the conventional gusset bag, so that the resin film used as a material can also be vertically long. Therefore, there is an advantage that a large-capacity gusset bag can be manufactured even when the gusset bag of the present embodiment is manufactured using a roll-shaped resin film having a length limitation in the width direction.

FIG. 3 shows a cross-sectional view of the gusset bag in a folded state. Further, FIG. 4 shows a cross-sectional view of the gusset bag inflated.
The first side wall 18 connects one side end 12a in the width direction of the front wall 12 and one side end 13a in the width direction of the rear wall 13. The first side wall 18 is a gusset portion that is folded inward in the width direction at at least one position between these side end portions 12a and 13a.
The second side wall 19 connects between the other side end 12b of the front wall 12 in the width direction and the other side end 13b of the rear wall 13 in the width direction. The second side wall 19 is a gusset portion that is folded inward in the width direction at at least one position between these side end portions 12b and 13b.

In the side walls 18 and 19, each of the gusset portions folded inward in the width direction is composed of different side films 14 and 15. In this case, the side films 14 and 15 can be made of a film having substantially the same width as the front wall 12 and the rear wall 13. Each of the side films 14 and 15 has fold lines 14c and 15c at a portion to be folded inward in the width direction. Further, the side film 14 and 15 have front side end portions 14a and 15a and rear side end portions 14b and 15b on both sides via the folding lines 14c and 15c. Since the gusset bag is composed of 6 films (the same number as N described later), the circumference is 6 times the film width in the inflated state as shown in FIGS. 2 and 4, and a large-capacity bag can be obtained. can get.

Of the front side film 14, the front side end portion 14a is joined to the side end portions 12a and 12b of the front wall 12, respectively. The rear side end portion 14b of the front side film 14 is joined to the front side end portion 15a of the rear side film 15, respectively. Of the rear side film 15, the rear side end portion 15b is joined to the side end portions 13a and 13b of the rear wall 13, respectively. In this way, the side end portions 14a, 14b of the front side film 14 are joined to the adjacent front wall 12 and the side end portions 12a, 12b, 15a of the side surface film 15. Further, the side end portions 15a, 15b of the rear side film 15 are joined to the adjacent rear surface wall 13 and the side end portions 13a, 13b, 14b of the side surface film 14. Each of the seal portions of the side end portions 12a, 12b, 13a, 13b, 14a, 14b, 15a, and 15b is configured by a seal in which the inner surfaces of the film face each other.

Instead of joining the side films 14 and 15 at the sealing portion, each side wall 18 and 19 can be composed of one film. In this case, each side wall 18 and 19 is composed of one film folded in a W shape instead of the two side films 14 and 15 folded in a V shape, and is inward in the width direction. It has fold lines that are folded and fold lines that are folded outward in the width direction alternately. A seal portion may be provided along the fold line that is folded outward in the width direction, or the outer seal portion may be omitted.

The number of places where the first side wall 18 is folded inward in the width direction of the gusset is G1, the number of places where the second side wall 19 is folded inward in the width direction is G2, and the front surface thereof. The number obtained by adding the number of the wall 12 and the rear wall 13 (that is, 2) is N = G1 + G2 + 2, and in the case of the present embodiment, G1 = 2, G2 = 2, and N = 6.

The number G1 of the gussets of the first side wall 18 and the number of gussets G2 of the second side wall 19 may be different, but it is preferable that the number is the same as G1 = G2. N is a number greater than 4, and may be, for example, 5-10. N may be 7, 8, 9, or the like, with 6 to 8 being more preferable. If at least one of G1 or G2 is 2 or more, one of G1 or G2 may be 1. In the case of the gusset bag disclosed in Patent Documents 1 and 2, G1 = 1, G2 = 1, and N = 4.

At the upper and lower ends of the front wall 12 and the rear wall 13, the angle θ formed by the hypotenuse seal portions 11b corresponding to both sides in the width direction is, for example, 80 ° or less, and can be 72 ° or less. The lower limit of θ can be 20 ° or more, preferably 30 ° or more. When N = 5, θ can be about 72 °, and when N = 6, θ can be about 60 °. Nθ, which is the product of the angle θ and the number N, is in the range of 280 to 400 °. As a result, when the gusset bag 10 is inflated as shown in FIGS. 2 and 4, the N wall surfaces form a substantially N-square columnar tubular surface as shown in FIG. 3, and the upper and lower ends of the N wall surfaces are formed. However, it constitutes an end face of approximately 360 °. Here, the end face formed by the upper end portion is the top surface, and the end face formed by the lower end portion is the bottom surface. When the value of Nθ is 360 °, the top and bottom surfaces of the inflated gusset bag approach a flat surface. When the value of Nθ is less than 360 °, the top and bottom surfaces of the inflated gusset bag become convex or concave. When it becomes convex, the top surface becomes a polygonal pyramid, and it becomes a shape like a pointed hat. When the value of Nθ is larger than 360 °, the top surface and the bottom surface tend to have wrinkled shapes. Nθ is preferably 300 ° or more, and more preferably 320 ° or more. By taking such a value, the top surface and the bottom surface of the gusset bag approach a flat surface. Further, Nθ is preferably 400 ° or less, and more preferably 360 ° or less. By taking such a value, it is possible to obtain a gusset bag that does not have wrinkles on the top surface or the bottom surface.

The gusset bag 10 of the present embodiment is not limited to a transport container such as a bag-in-box and a packaging container such as a storage container, but can also be used as a culture container, a reaction container and the like. Since the gusset bag 10 of the present embodiment has a substantially cylindrical shape in an inflated state, it can be perfectly accommodated in a cylindrical outer container or outer shell container. Since the inner surface is substantially columnar, pouring, stirring, mixing, etc. are easy.

Further, as shown in FIG. 5, the upper and lower ends of the gusset bag 10 are joined between the left and right hypotenuse seal portions 11b and 11b so that the inner surface of the front wall 12 and the inner surface of the rear wall 13 face each other directly. It may have a tip seal portion 11c. Thereby, the side films 14 and 15 can be easily folded between the front wall 12 and the rear wall 13.

The gusset bag 10 may be a multiple bag such as a double bag or a triple bag. In this case, it is more preferable because the liquid does not easily leak. When the gusset bag 10 of the present embodiment is a multiple bag, it is preferable that the number and position of the gussets correspond to each other when each side surface portion of the inner gusset bag and each side surface portion of the outer gusset bag overlap.

The material of the film constituting the gusset bag 10 is not particularly limited, but by using a thermoplastic resin such as polystyrene, polyamide, polyester, or polyolefin, or a laminated film thereof, it is easy to guarantee the product quality of the manufactured pharmaceutical product. can do. In the case of multiple bags, the material of the inner gusset bag and the outer gusset bag may be different. For example, the inner gusset bag may be composed of a single-layer film of polyethylene (PE), and the outer gusset bag may be composed of a multilayer film of polyethylene (PE) / nylon (Ny) / polyethylene terephthalate (PET). The thickness of each film may be different depending on the portion constituting the gusset bag.

The capacity (size) of the gusset bag 10 is not particularly limited, but can be, for example, 0.1 L to 5000 L, preferably 100 to 3000 L. The gusset bag 10 may be single-use (disposable). For example, when the capacity is 20 L, it may be about 160 mm in width and about 400 mm in length. Further, when the capacity is 200 L, it may be about 800 mm in width and about 2800 mm in length. If the dimensions are further increased, it is possible to handle a capacity of about 500 to 3000 L.

A plurality of tubes 17 can be connected to the gusset bag 10 for the purpose of adding or taking out contents such as a medium and an atmospheric gas. Further, the gusset bag 10 can be provided with a spout 16. The spout 16 may be provided with a cock, a cap, a tapered spout, or the like. The tube 17 may be connected to the spout 16. In the case of the present embodiment, the spout 16 is fixed to the gusset bag 10 by sealing the base of the spout 16 on the back side of the film.

The material of the tube is not particularly limited, but a material having excellent chemical resistance, weather resistance, etc., such as silicone and thermoplastic elastomer, is preferable. The tube may be provided with a filter, a flow monitor, a flow meter, a valve, a pump, or the like.

The gusset bag of the present embodiment is suitable for cell culture (production of cultured cells) or storage, and can be stirred or stored in the manufacturing process of products such as biopharmacy and regenerative medicine.
Biopharmacy is a drug manufactured, extracted, semi-synthesized, etc. using living organisms. Regenerative medicine products are (1) human or animal cells that have been processed by culture, etc., and that reconstruct, repair, or form the structure or function of the body, or (2) human or animal products. The cells are processed by culture or the like and are used for the purpose of treating or preventing diseases, or (3) those which are introduced into human cells and used for the purpose of gene therapy.
There is an advantage that shear stress is less likely to be applied when the cells, pharmaceuticals, pharmaceuticals, intermediates of products such as regenerative medicine are placed in the gusset bag of the present embodiment, and the cells are cultured or stirred. Further, when the gusset bag of the present embodiment is stored in the gusset bag of the present embodiment, the ratio of the wall surface to the volume of the contents of the gusset bag of the present embodiment is smaller than that of the cubic gusset bag, so that deterioration due to the surface of the container occurs. It has the advantage of being difficult.

Using the gusset bag of the present embodiment, cells can be cultured using, for example, a shaking type culture device 30 as shown in FIG. 7 having an outer shell container 20 as shown in FIG. When the shaking culture is performed using the shaking type culture device 30 using the gusset bag of the present embodiment, the cells contained in the liquid inside the gusset bag serving as the culture bag are less likely to be sheared by stirring. Become. This is because the gusset bag of the present embodiment is a gusset bag closer to a columnar shape than the conventional cubic gusset bag, so that it is easy to fit in the outer shell container, wrinkles are less likely to occur in the gusset bag, and the gusset bag is shaken and stirred. This is because the internal solution tends to flow when the above is performed, and low shear stress can be realized. As a result, cell death in shaking and stirring can be prevented, and efficient culture can be achieved.
FIG. 6 is an example of the outer shell container 20 included in the shaking type culture apparatus shown in FIG. 7. The outer shell container 20 can accommodate the entire gusset bag 10 in order to protect the gusset bag 10. The configuration of the outer shell container is not particularly limited, but the outer shell container 20 of FIG. 6 has a container body 21 having a side wall portion 21a and a bottom wall portion 21b, and a lid portion 22 for closing the upper opening of the side wall portion 21a. Be prepared. If the side wall portion 21a can accommodate all or most of the gusset bag 10 in the vertical direction, the lid portion 22 may be omitted. The lower part of the side surface of the gusset bag 10 may be covered with the container body 21, and the upper part of the side surface of the gusset bag 10 may be covered with the lid portion 22.

In order to suppress internal temperature fluctuations, it is preferable to close the opening for taking the culture bag in and out of the outer shell container 20 with the lid portion 22. The lid portion 22 may be a removable type that can be separated from the container main body 21, or may be connected to the container main body 21 so as to be openable and closable by a hinge or the like. The material of the outer shell container 20 (for example, the container body 21 and the lid portion 22) preferably has a hardness (rigidity) capable of protecting the culture bag, and for example, a metal such as stainless steel, resin, wood, laminated wood, etc. Examples include composite materials such as fiber reinforced plastics. The internal space of the outer shell container is cylindrical, and it is preferable that the circular portion of the cylinder is located on the bottom surface of the outer shell container from the viewpoint of stirring efficiency. The ratio of the diameter (inner diameter) of the circle located on the bottom surface of the cylinder in the internal space to the height of the cylinder is, for example, 1: 2 to 2: 1. The inner diameter and height may be the same (ratio 1: 1).

When the gusset bag 10 is used as a culture bag, the contents preferably contain a liquid (medium) and a gas. The volume ratio of gas to liquid (gas-liquid volume ratio) is not particularly limited, and the amount of liquid may be larger than that of gas, the amount of gas may be larger than that of liquid, or the ratio may be about half. The gas-liquid volume ratio is preferably in the range of, for example, 1: 9 to 9: 1. It is preferable that the gusset bag 10 is in close contact with the outer shell container 20 and the outer shell container 20 supports the side surface of the gusset bag 10 in at least the entire or most of the range in which the gusset bag 10 contains a liquid (medium). In the portion where the gas is contained in the gusset bag 10, the upper portion (part) of the gusset bag 10 may be exposed on the upper (outside) of the outer shell container 20.

The side wall portion 21a has a window 23 for visually confirming the amount (liquid level) of the contents (liquid level) of the culture bag contained in the outer shell container 20. The window 23 is provided as a slit-shaped cut in the vertical direction of the side wall portion 21a. The window 23 may be opened or closed with a transparent material. Further, the bottom wall portion 21b and the lid portion 22 have holes 24 and 25 for passing the tube 17. When culturing using the gusset bag of the present embodiment, the tube 17 is connected to the spout 16 on the bottom surface of FIG. 2, and the tube 17 is passed through the hole 25 of the outer shell container 20 of the incubator shown in FIG. 6 and stirred. Culture can be performed. This facilitates the discharge of the contents of the gusset bag from the bottom surface through the tube, which is preferable.

As an example of the shaking operation of the outer shell container, the planar shape of the outer shell container 20 is a circle, the three-dimensional shape is a cylinder with the central axis oriented substantially up and down, and the center of the outer shell container 20 rotates on an orbit with a radius R. You may (revolve). The amplitude of the shaking is, for example, twice the radius R. Further, the outer shell container 20 may be rotated (rotated) around the center.
When shaking, the outer shell container 20 containing the culture bag is preferably rotated in the horizontal direction.
The gusset bag of the present embodiment can be used as an outer box by putting it in various outer shell containers. Examples of the outer shell container include a spherical shape, a columnar shape, a polygonal columnar shape, a cube, and the like, but it is particularly preferable that the outer shell container has a cylindrical shape or a polygonal columnar shape, and the outer shell container has a shape close to the shape of a gusset bag. preferable. The columnar or polygonal outer shell container also has the advantage of increasing the strength when the outer shell containers are stacked vertically. The material of the outer shell container is not particularly limited, and examples thereof include metal, plastic, corrugated cardboard, and wood.

Although the present invention has been described above based on the preferred embodiment, the present invention is not limited to the above-described embodiment, and various modifications can be made without departing from the gist of the present invention.

10 ... Gazette bag, 11 ... Seal part, 11b ... Hypotenuse seal part, 12 ... Front wall, 13 ... Rear wall, 14, 15 ... Side film, 16 ... Spout, 17 ... Tube, 18 ... First side wall, 19 ... 2nd side wall, 20 ... outer shell container, 30 ... shaking culture device, 31 ... base, 32 ... frame, 33 ... power source.

Claims (6)

Front and rear walls facing each other,
A gusset portion that connects one side end portion in the width direction of the front wall and one side end portion in the width direction of the rear surface wall, and is folded inward in the width direction at at least one place between them. The first side wall that constitutes
A gusset portion that connects between the other side end portion in the width direction of the front wall and the other side end portion in the width direction of the rear surface wall, and is folded inward in the width direction at at least one place between them. With a second side wall, which constitutes
The front wall and the rear wall have a hypotenuse seal portion sealed along a hypotenuse portion whose width becomes narrower toward both upper and lower ends.
The number of points where the gusset of the first side wall is folded inward in the width direction is G1, and the number of places where the gusset of the second side wall is folded inward in the width direction is G2. The number obtained by adding the numbers of the front wall and the rear wall is N = G1 + G2 + 2, and the angle θ formed by the hypotenuse seal portions corresponding to both sides in the width direction is 80 ° or less, and at least one of the G1 or the G2. Is 2 or more, N is 5 or more, and Nθ, which is the product of θ and N, is in the range of 280 to 400 ° .
The top surface formed by the upper ends of the upper and lower ends has a first spout on either the front wall or the rear wall between the hypotenuse seals facing in the width direction. A tube is attached to the first spout.
The bottom surface formed by the lower ends of the upper and lower ends has a second spout on either the front wall or the rear wall between the hypotenuse seals facing in the width direction. A tube is attached to the second spout,
Each of the upper end portion and the lower end portion is characterized by having a tip seal portion in which the inner surface of the front wall and the inner surface of the rear wall are directly opposed and joined between the hypotenuse seal portions. Gazette bag to do. Of the first side wall or the second side wall, the side wall having two or more points folded inward in the width direction is composed of side films having different gusset portions.
Each of the side films has a fold line at a portion to be folded inward in the width direction, and both side ends via the fold line are sequentially adjacent to each other between the front wall and the rear wall. The gusset bag according to claim 1, wherein the side end portions of the films to be formed are joined to each other by sealing the inner surfaces of the films. The gusset bag according to claim 1 or 2, wherein the first side wall and the second side wall have the same number of points to be folded inward in the width direction. The gusset bag according to any one of claims 1 to 3, wherein N is 5 to 10. The gusset bag according to any one of claims 1 to 4, which is a gusset bag for manufacturing or storing any of cultured cells, biopharmacy, regenerative medicine and the like products. A shake-type incubator comprising the gusset bag according to any one of claims 1 to 5 and an outer shell container for accommodating the gusset bag.

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