JP6991432B1 - Dwarf lilyturf origin identification method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for discriminating the production area of Dwarf lilyturf. SOLUTION: A step of putting Janohige powder and an ethanol aqueous solution having a volume percent concentration of 85% to 95% in a processing box 1, heating and refluxing to completely extract the filtrate, and concentrating the filtrate to non-ethanol. Then, ionized water is added and gently precipitated at 2 to 10 ° C., the supernatant is picked up, filtered, and the content of offiopogonin in the Janohige powder is measured by high-speed liquid chromatography. [Selection diagram] Fig. 3

Description

The present invention belongs to the technical field of Dwarf lilyturf producing area identification, and particularly relates to a method for distinguishing Dwarf lilyturf producing area.

Dwarf lilyturf (also known as snake beard, or wheat gate winter) is a dried mass root of the lilyturf lilyturf Ophiopogon japonicus (Lf) Ker-Gawl. Dwarf lilyturf can be classified into Zhejiang Dwarf lilyturf and Sichuan Dwarf lilyturf according to the place of origin and type. Studies show that some pharmacological adaptive effects of Zhejiang Dwarf lilyturf are superior to Sichuan Dwarf lilyturf. However, according to market research
Due to the scarcity of sources of Zhejiang Dwarf lilyturf and the high price, there are currently relatively few Chinese herbal medicines that use Zhejiang Dwarf lilyturf as a medicinal product. Therefore, it is necessary to confirm whether or not Zhejiang Dwarf lilyturf is used as a raw material in Chinese herbal preparations, and it is also necessary to detect and confirm it as a product preparation. It is hoped that the excellent students of Zhejiang Dwarf lilyturf can be embodied in the Chinese herbal medicines that Zhejiang Dwarf lilyturf makes medicinal.

However, traditionally, neither national and local standards nor pharmaceutical research have extended to the method of identifying Zhejiang Dwarf lilyturf in product formulations. Documents and patents describe methods for appraising Zhejiang Dwarf lilyturf crude drugs, but chemical experimental methods such as appearance discrimination and liquid phase fingerprint spectra are not suitable for appraisal of Dwarf lilyturf powder. At present, there is no clearly effective method for distinguishing between Zhejiang dwarf lilyturf and Sichuan dwarf lilyturf in Chinese herbal preparation products.

The present invention provides a method for distinguishing the origin of Dwarf lilyturf in order to overcome the shortage of prior art.

In order to achieve the above object, the present invention adopts the following technical plan.
How to identify the origin of Dwarf lilyturf
a) A treatment box containing Dwarf lilyturf powder and an aqueous ethanol solution having a volume percent concentration of 85% to 95% (
1) When placed in 1), heated under reflux and completely extracted, the step of obtaining the filtrate a and
b) A step of concentrating the filtrate a to non-ethanol, adding ionized water and gently precipitating at 2 to 10 ° C., picking up the supernatant and filtering to obtain the filtrate b.
c) Porous polymer bead column chromatography was performed on the filtrate b, and sequentially deionized water, an aqueous ethanol solution having a volume percent concentration of 15% to 25%, an aqueous solution of 25% to 35% ethanol having a volume percent concentration, and a volume percent concentration. The process of collecting the effluent by performing gradient elution using a 35% to 45% aqueous ethanol solution, and
d) A step of measuring the content of offiopogonin D in Dwarf lilyturf powder by high performance liquid chromatography, and
e) Dragon brain glycoside (l-borneol 2-O-β-D) in the effluent in step c
-Apioflanosyl- (1 → 6) -β-D-glucopyranoside) is inspected, and the content of offiopogonin D in Zhejiang dwarf lilyturf having the same mass as the content of detected offiopogonin D in step d. If the test dwarf lilyturf contains dragon brain glycoside, it is judged that the test dwarf lilyturf contains a component of Zhejiang dwarf lilyturf, and the content of offiopogonin D in the test dwarf lilyturf is the same mass. If the content of Ofiopogonin D in Zhejiang Dwarf lilyturf is higher than that of Dwarf lilyturf, it includes the step of determining that Sichuan Dwarf lilyturf is mixed in the test Dwarf lilyturf.
The porous polymer beads in step c are D101 type large pore resin or D1400 type large pore resin, and the bed volume of the porous polymer beads is 10-20% of the volume of the filtrate b.
Gradient elution in step c is carried out sequentially using deionized water, an ethanol aqueous solution having a volume percent concentration of 20%, an ethanol aqueous solution having a volume percent concentration of 30%, and an ethanol aqueous solution having a volume percent concentration of 40%.
The lid plate 2 is provided in the processing box 1 in the step a, the first concave groove 101 is provided in the inner wall of the processing box 1, the heating block 18 is provided in the first concave groove 101, and the reflux pipe 21 is provided in the lid plate 2. Is provided,
The lid plate 2 is further provided with a gas cylinder 26, the piston rod of the gas cylinder 26 is provided with a press plate 3 that cooperates with the processing box 1, and the press plate 3 is provided with a plurality of first through holes 301. A first motor for rotationally driving the press plate 3 is provided on the side wall of the processing box 1, a first connection block 11 is provided on the side wall of the processing box 1, and a first moving chamber is provided in the first connection block 11. 112 is provided, a second connection block 19 that cooperates with a plurality of press plates 3 is provided in the first moving chamber 112, and a drain port 1121 is provided on the side wall opposite to the lid plate 2 of the first moving chamber 112. 271 is provided on the lid plate 2, the first sealing plate 27 is provided on the opposite side of the launch port 271 from the press plate 3, and the supply port 102 is provided on the side wall of the processing box 1. And supply port 10
A third sealing plate is provided in 2, and the press plate 3 is moved and driven toward the lid plate 2 by the gas cylinder 26 in order to acquire the mixed solution, and the press plate 3 is closer to the lid plate 2 of the supply port 102. When moved to one side, the Janohige powder is put into the processing box 1 through the supply port 102, and after the Janohige powder is put in, the ethanol aqueous solution is made to enter the processing box 1 from the launch port 271 to enter the Janohige powder and the ethanol. When the addition of the aqueous solution is completed, the mixed solution in the processing box 1 is heated by the heating block 18, the press plate 3 is rotationally driven by the first motor, the mixed solution is stirred by the press plate 3, and the steam is recirculated in the reflux tube 21. When it gets inside, liquefies, drops into the processing box 1, and the heating and refluxing is completed, the press plate 3 is pushed by the gas cylinder 26 and moved to the opposite side of the lid plate, and the press plate 3 is used to remove the Janohige powder in the solution. The powder is pushed downward while being pushed, and the beard powder is pressed to the bottom of the processing box 1, the second connection block 19 and the press plate 3 cooperate with each other, and the mixed solution on the side closer to the lid plate of the press plate 3 is the first. 1 Enters the moving chamber 112, the solution is discharged from the drain port 1121, and the filtrate a is obtained.

Borneol 7-O- [β-D-apiofuranosyl- (
1-> 6)]-β-D-glucopylanoside: l-Borneoll 2-O-β
-Effective identification of the type of Dwarf lilyturf by differentiating the origin of Dwarf lilyturf by inspection for D-Apioflanosyl- (1 → 6) -β-D-glucopyranoside) and Ofiopogonine D. Can be done. Then, it can be determined whether or not the Dwarf lilyturf powder to be inspected is a mixed molding. This can improve the reliability of the Dwarf lilyturf discrimination and the accuracy of the Dwarf lilyturf origin discrimination. By the interaction between the press plate and the gas cylinder, the dwarf lilyturf powder is pressed against the bottom of the processing box after the heating and reflux in the processing box is completed, thereby preventing the filtration holes from being blocked by the dwarf lilyturf powder during filtration of the dwarf lilyturf powder. Therefore, the solution can be smoothly discharged from the drain port, which reduces the difficulty of subsequent cleaning. Due to the rotation drive method of the press plate by the first motor, the press plate can also have a function of stirring the solution in the process of heating and refluxing. This allows the Dwarf lilyturf powder to be evenly contacted with the solution, ensuring the effectiveness of the extract. Since the dwarf lilyturf powder is placed in the processing box via the supply port, it is made as if the dwarf lilyturf powder was under the press plate even at the time of startup, and the dwarf lilyturf in the processing box is lowered as the press plate is lowered. The powder can be moved to the bottom of the processing box to separate the dwarf lilyturf powder from the solution, and the solution can be quickly discharged from the processing box to improve the efficiency of discrimination against dwarf lilyturf.

A first moving groove 301 is opened on the bottom of the press plate 3 on the opposite side of the lid plate 2, a first stirring rod 31 is provided in the first moving groove 301, and a first slide is provided on the side wall of the first stirring rod 31. Block 311
Is provided, and a first sliding groove 3011 that cooperates with the first sliding block 311 is provided on the inner wall of the first moving groove 301.

The press plate 3 is provided with a second moving groove 302, a first connecting rod 33 is provided in the second moving groove 302, and a first through groove 3021 is provided on one side of the second moving groove 302 near the lid plate. , The first connection rod 33 is provided with a guide groove 330 that cooperates with the first through groove 3021, and the second connection block 1
A first through chamber 190 that cooperates with the first connecting rod 33 is provided in 9, and the first connecting rod 33 is pushed toward the outside of the second moving groove 302 on the opposite side of the press plate 3 from the lid plate. The first to move
A push plate 32 is provided.

The press plate 3 is moved and driven upward by the gas cylinder 26, and the press plate 3 is used as the supply port 10.
Located above 2, the first stirring rod 31 extends from the inside of the first moving groove 301, the press plate 3 is rotationally driven by the first motor, and the first stirring rod 31 processes with the rotation of the press plate 3. When the solution in the box 1 is agitated, the solution heats and refluxes in the processing box 1, and the heating and refluxing of the solution is completed.
When the press plate 3 is rotationally driven by the first motor and the first connecting rod 33 is rotated to the position corresponding to the second connecting block 19, the gas cylinder 26 moves the press plate 3 to the opposite side of the lid plate. When the press plate 3 moves toward the opposite side of the lid plate to the bottom of the processing box 1,
The first connecting rod 33 moves to one side of the second connecting block 19, and the first push plate 32 and the first stirring rod 31
Is pressed onto the Dwarf lilyturf powder, the first stirring rod 31 enters the first moving groove 301, the first pushing plate 32 moves toward the press plate 3, and the first connecting rod 33 moves into the second moving groove. Extending from inside 302, the first connecting rod 33 is inserted into the first through chamber 190, and the solution on one side of the press plate 3 near the lid plate enters the guide groove 330 from the first through groove 3021. The water flow is the first along the guide groove 330.
It enters the moving chamber 112, the extract flows out from the drainage port 1121, and the dwarf lilyturf powder is in the processing box 1.
It stays inside to obtain the solution a alone.

The advantageous effects of the present invention are as follows. Borneol 7-O- [
β-D-apiofurananosyl- (1-> 6)]-β-D-glucophyran
oside: l-bornolol 2-O-β-D-apioflanosyl- (1 → 6) -β-D
-The type of Dwarf lilyturf can be effectively identified by differentiating the production area of Dwarf lilyturf by the inspection for (glucopyranoside) and Ofiopogonine D. Then, it can be determined whether or not the Dwarf lilyturf powder to be inspected is a mixed molding. This can improve the reliability of the Dwarf lilyturf discrimination and the accuracy of the Dwarf lilyturf origin discrimination.

It is a block diagram of the reaction box of this invention. It is a front view of the reaction box of this invention. It is sectional drawing which follows AA of FIG. It is an enlarged view of A of FIG. It is an enlarged view of Q of FIG. It is sectional drawing which follows BB of FIG. It is an enlarged view of B of FIG. FIG. 3 is a cross-sectional view taken along the line CC of FIG. It is an enlarged view of C of FIG. It is sectional drawing which follows the DD of FIG. It is an enlarged view of D of FIG. It is sectional drawing which follows EE of FIG. It is an enlarged view of E of FIG. It is a right side view of the reaction box of this invention. FIG. 3 is a cross-sectional view taken along the line FF of FIG. It is an enlarged view of F of FIG. It is an enlarged view of G of FIG. It is an enlarged view of H of FIG. FIG. 6 is a cross-sectional view taken along the line LL of FIG. It is an enlarged view of I of FIG. FIG. 6 is a cross-sectional view taken along the line HH of FIG. It is an enlarged view of J of FIG. It is an enlarged view of K of FIG. FIG. 6 is a cross-sectional view taken along the line I-I of FIG. It is an enlarged view of L of FIG. 24. FIG. 6 is a cross-sectional view taken along the line JJ of FIG. It is an enlarged view of M of FIG. 26. FIG. 6 is a cross-sectional view taken along the line KK of FIG. It is an enlarged view of N of FIG. 28. It is an enlarged view of O of FIG. 28. It is an enlarged view of P of FIG.

The method of distinguishing the production area of Dwarf lilyturf is as follows:
a) A treatment box containing Dwarf lilyturf powder and an aqueous ethanol solution having a volume percent concentration of 85% to 95% (
When it is put in 1), heated under reflux and completely extracted, the filtrate a is obtained.
b) After concentrating the filtrate a to non-ethanol, add ionized water and gently precipitate at 2 to 10 ° C., take up the supernatant and filter to obtain the filtrate b.
c) Porous polymer bead column chromatography was performed on the filtrate b, and sequentially deionized water, an aqueous ethanol solution having a volume percent concentration of 15% to 25%, an aqueous solution of 25% to 35% ethanol having a volume percent concentration, and a volume percent concentration. Gradient elution is performed using a 35% to 45% aqueous ethanol solution to collect the effluent, and
d) Measuring the content of offiopogonin D in Dwarf lilyturf powder by high performance liquid chromatography, and
e) Dragon brain glycoside (l-borneol 2-O-β-D) in the effluent in step c
-Apioflanosyl- (1 → 6) -β-D-glucopyranoside) is inspected, and the content of offiopogonin D in Zhejiang dwarf lilyturf having the same mass as the content of detected offiopogonin D in step d. If the test dwarf lilyturf contains dragon brain glycoside, it is judged that the test dwarf lilyturf contains a component of Zhejiang dwarf lilyturf, and the content of offiopogonin D in the test dwarf lilyturf is the same mass. If the content of Ofiopogonin D in Zhejiang Dwarf lilyturf is higher than that of Dwarf lilyturf lilyturf, it includes determining that Sichuan Dwarf lilyturf is mixed in the Dwarf lilyturf to be tested.
The volume of the extract used in the step a is 2 to 4 L / kg in terms of the unit mass of the beard powder, and the volume of deionized water added in the step b is 2 to 4 L / kg in the unit of the beard lump root. The porous polymer beads in the above are D101 type large pore resin or D1400 type large pore resin, the bed volume of the porous polymer beads is 10-20% of the volume of the filtrate b, and the gradient elution in the step c is sequentially removed. This is performed using ionized water, an aqueous ethanol solution having a volume percent concentration of 20%, an aqueous ethanol solution having a volume percent concentration of 30%, and an aqueous ethanol solution having a volume percent concentration of 40%.

As shown in FIGS. 1 to 31, a lid plate 2 is provided in the processing box 1 in the process a, a first concave groove 101 is provided in the inner wall of the processing box 1, and a heating block 18 is provided in the first concave groove 101. And the lid plate 2
Is provided with a reflux tube 21. A gas cylinder 26 is further provided on the lid plate 2. The piston rod of the gas cylinder 26 is provided with a press plate 3 that cooperates with the processing box 1, a plurality of first through holes 301 are provided in the press plate 3, and the press plate 3 is rotated on the side wall of the processing box 1. A first motor for driving is provided, a first connection block 11 is provided on the side wall of the processing box 1, a first moving chamber 112 is provided in the first connection block 11, and the inside of the first moving chamber 112 is provided. A second connection block 19 that cooperates with a plurality of press plates 3 is provided, a drain port 1121 is provided on the side wall opposite to the lid plate 2 of the first moving chamber 112, and a launch port is provided on the lid plate 2. 271 is provided, a first sealing plate 27 is provided on the opposite side of the launch port 271 from the press plate 3, a supply port 102 is provided on the side wall of the processing box 1, and a third sealing plate is provided at the supply port 102. It is provided. In order to obtain the mixture, the gas cylinder 26 moves and drives the press plate 3 toward the lid plate 2, and when the press plate 3 moves to one side of the supply port 102 near the lid plate 2, the dwarf lilyturf powder is supplied to the supply port. 102
After being put into the processing box 1 and the dwarf lilyturf powder is put in, the ethanol aqueous solution is made to enter the processing box 1 from the launch port 271. When the addition of the Janohige powder and the aqueous ethanol solution is completed, the mixed liquid in the processing box 1 is heated by the heating block 18, the press plate 3 is rotationally driven by the first motor, the mixed liquid is stirred by the press plate 3, and steam is used. Enters the reflux tube 21, liquefies, and drops into the processing box 1. When the heating and refluxing is completed, the gas cylinder 26 pushes the press plate 3 and moves it to the opposite side of the lid plate, and the press plate 3 pushes the dwarf lilyturf powder in the solution and moves it downward, and the dwarf lilyturf powder moves downward while pushing the bottom of the processing box 1. Is pressed down to. The second connection block 19 and the press plate 3 form a cooperation, the mixed liquid on the side closer to the lid plate of the press plate 3 enters the first moving chamber 112, the solution is discharged from the drain port 1121, and the filtrate is discharged. a can be obtained.

A first moving groove 301 is opened on the bottom of the press plate 3 on the opposite side of the lid plate 2, a first stirring rod 31 is provided in the first moving groove 301, and a first slide is provided on the side wall of the first stirring rod 31. Block 311
Is provided, and a first sliding groove 3011 that cooperates with the first sliding block 311 is provided on the inner wall of the first moving groove 301. By installing the first sliding block 311 and the first sliding groove 3011, it is possible to prevent the first stirring rod from being detached from the first moving groove 301. When the press plate moves to the bottom of the processing box, the first stirring rod 31 automatically enters the first moving groove 301.
The press plate 3 is provided with a second moving groove 302, a first connecting rod 33 is provided in the second moving groove 302, and a first through groove 3021 is provided on one side of the second moving groove 302 near the lid plate. , The first connection rod 33 is provided with a guide groove 330 that cooperates with the first through groove 3021, and the second connection block 1
A first through chamber 190 that cooperates with the first connecting rod 33 is provided in 9, and the first connecting rod 33 is pushed toward the outside of the second moving groove 302 on the opposite side of the press plate 3 from the lid plate. The first to move
A push plate 32 is provided. The gas cylinder 26 moves and drives the press plate 3 upward. The press plate 3 is located above the supply port 102, and the first stirring rod 31 extends from the inside of the first moving groove 301. The press plate 3 is rotationally driven by the first motor, and the first stirring rod 31 agitates the solution in the processing box 1 together with the rotation of the press plate 3. The solution heats and refluxes in the processing box 1. When the heating and reflux of the solution is completed, the press plate 3 is rotationally driven by the first motor, and the first
When the connecting rod 33 rotates to the position corresponding to the second connecting block 19, the gas cylinder 26 moves and drives the press plate 3 to the opposite side to the lid plate. When the press plate 3 moves toward the opposite side of the lid plate to the bottom of the processing box 1, the first connecting rod 33 moves to one side of the second connecting block 19. The first push plate 32 and the first stirring rod 31 are pressed onto the Dwarf lilyturf powder. The first stirring rod 31 enters the first moving groove 301, the first pushing plate 32 moves toward the pressing plate 3, and the first pushing plate 32 moves toward the pressing plate 3.
The first connecting rod 33 extends from the inside of the second moving groove 302, the first connecting rod 33 is inserted into the first through chamber 190, and the solution on one side of the press plate 3 near the lid plate is the first through groove. Guide groove 33 from 3021
Entering 0, the water flow enters the first moving chamber 112 along the guide groove 330, the extract flows out from the drain port 1121, and the dwarf lilyturf powder stops in the processing box 1 to independently charge the solution a. can get.

The 16th moving groove is provided at the bottom of the press plate, the 3rd connecting rod 321 is provided at the 1st push plate, the 3rd connecting rod penetrates the 16th moving groove, and the top of the 3rd connecting rod is the first. 9 Connection spring 322
Is provided, a 17th moving groove communicating with the 16th moving groove is provided on the side wall of the second moving groove 302, a fourth connecting rod 331 is provided on the side wall of the first connecting rod, and the fourth connecting rod is the 17th. A tenth connection spring 332 is provided at one end of the fourth connection rod so as to penetrate the inside of the moving groove. When the first push plate moves upward, the third connecting rod moves toward the inside of the 16th moving groove. The air in the 16th moving groove is pushed by the 3rd connecting rod to enter the 17th moving groove. The fourth connecting rod is moved by the atmospheric pressure. By moving the first connecting rod with the movement of the fourth connecting rod,
The first connecting rod extends from the inside of the second moving groove 302 to form a connection with the second connecting block. It can be ensured that the width of the guide groove 330 is larger than the width of the first through groove 3021 and that all the solutions entering from the first through groove 3021 enter the guide groove 330.

A third connection block 261 is provided at one end of the piston rod of the gas cylinder 26 on the side closer to the press plate, a fourth connection block 263 is provided on the side opposite to the lid plate of the third connection block 261, and the press plate 3 is provided with a fourth connection block 263. A second concave groove 303 that cooperates with the connection block 263 is provided, and a third groove is provided.
A connection ring 264 is fitted onto the connection block 261, a screw that interacts with the connection ring 264 is provided on the inner wall of the second concave groove 303, and a first connection plate 26 is provided on the side wall of the fourth connection block 263.
31 is provided, a second connection plate 35 is provided in the second concave groove 303, and a first stop block 16 that cooperates with the press plate 3 is provided on the inner wall of the processing box 1. When the press plate 3 is moved and driven toward the lid plate by the gas cylinder 26, the first stop block 16 protrudes from the inner surface of the processing box, and the press plate 3 moves toward the lid plate to the first stop block 16 and comes into contact with the first stop block 16. When the press plate 3 touches the bottom of the first stop block 16, it cannot move toward the lid plate side. By driving the piston rod of the gas cylinder 26, the fourth connection block 263 is moved toward the lid plate side with respect to the press plate 3, and the first connection plate 2631 is moved toward the lid plate side of the second connection plate 35. When the press plate 3 is rotationally driven by the first motor, the fourth connection block 263
Rotates on the lid plate side of the second concave groove 303. When the heating and reflux of the solution is completed, the gas cylinder 26
Drives the piston rod downward. The fourth connection block 263 moves toward the opposite side of the lid plate together with the piston rod of the gas cylinder 26. When the fourth connection block 263 moves to the inner wall opposite to the lid plate of the second concave groove 303, the press plate 3 is rotationally driven by the first motor. When the second connection plate 35 rotates to one side of the first connection plate 2631, the first connection plate 2631 further stops the continuous rotation of the second connection plate 35. When the rotation of the press plate 3 is stopped, the first connecting rod 33 is positioned at a predetermined position. The gas cylinder 26 subsequently drives the piston rod to move toward the opposite side of the lid plate. The press plate 3 pushes the dwarf lilyturf powder in the processing box 1 toward the opposite side of the lid plate and moves it to the bottom of the processing box 1.
The solution in the upper layer is discharged from the drain port 1121 to obtain the filtrate a.

A first connection spring 262 is provided on the lid plate side of the connection ring 264, and a plurality of third moving grooves 2612 communicating with the first cavity 2610 and the first cavity 2610 are provided in the third connection block 261. A first stop rod 2611 is provided in the moving groove 2612, a second connection spring is provided in the first stop rod 2611, and a third is provided in the inner wall of the connection ring 264.
A plurality of fourth moving grooves 2641 corresponding to the moving groove 2612 are provided, and the fourth moving groove 2641 is provided.
A second balloon 266 is provided inside, and the connection ring 264 communicates with the fourth moving groove 2641.
A moving chamber 2642 is provided, a third connecting spring 2651 is provided on the side closer to the lid plate in the second moving chamber 2642, and a second push plate 265 is provided on the side opposite to the lid plate of the third connecting spring 2651.
Is provided, and the second push plate 265 penetrates the inside of the second moving chamber 2642. The fourth connection block 263 is provided with a fifth moving groove 2630 communicating with the first cavity 2610, the first connecting plate 2631 is provided in the fifth moving groove 2630, and the first connecting plate 2631 has a fourth connecting spring 2632. It is provided. When the press plate 3 is connected on the piston rod of the gas cylinder 26, the fourth connection block 263 is inserted into the second concave groove 303, and the connection ring 264 is located at the groove opening position of the second concave groove 303. At this time, the first stop rod 2611 is the fourth moving groove 26.
It is fitted into 41, and the connection ring 264 and the fourth connection block 263 form a rotation stopper.
When the press plate 3 is rotated, the connection ring 264 is fastened to the screw, and the connection ring 264 is fixed to the press plate 3. When the connection ring 264 is connected to the press plate 3, the connection ring 264 moves with respect to the press plate 3 toward the side opposite to the lid plate. The second push plate 265 is the support ring 3
4 It conflicts with the top and is moved toward the lid plate side while pushing the second push plate 265 by the support ring 34. The second push plate 265 blows the air in the second moving chamber 2642 into the balloon 266. 3rd moving groove 2 while pushing the 1st stop rod 2611 by the balloon 266
Move within 612. When the first stop rod 2611 moves, the air in the third moving groove 2612 is pushed, and the air in the third moving groove 2612 enters the first cavity 2610, and the first
The air in the cavity 2610 enters the fifth moving groove 2630, extends from the fifth moving groove 2630 while pushing the first connecting plate 2631 by the atmospheric pressure, stops the rotation of the press plate 3, and presses the press plate 3. And the connection with the piston rod of the gas cylinder 26 are completed. Since the first connection plate is located in the fifth moving groove 2630 when the press plate is assembled, the collision between the first connection plate and the second connection plate during the assembly of the press plate adversely affects the normal assembly of the press plate. It can be avoided.

An air outlet is provided at the top of the connection ring, and a fifth sealing plate 267 is provided at the air outlet. In order to remove the press plate, the fifth sealing plate is opened, the airflow in the second moving chamber 2642 is discharged from the air outlet, the balloon contracts, and the first stop rod is again in the fourth moving groove 2641. The first connection block and the connection ring form a rotation stopper. The first connection plate is the fifth moving groove 26
When the press plate is rotated inside the 30 and the press plate is removed from the connecting ring, the separation of the press plate and the gas cylinder is completed.

The second connecting plate 35 is provided with a first convex block 351, the first connecting plate 2631 is provided with a third concave groove that cooperates with the first convex block 351 and the first convex block 351 is provided with a sixth. A moving groove is provided, a stop spring 352 is provided in the sixth moving groove, a second stop block 353 is provided at one end of the stop spring 352, and a mutual cooperation with the first convex block 351 is provided on the inner wall of the third concave groove. A working seventh moving groove is provided, and the first convex block can move up and down along the inner wall of the seventh moving groove. An eighth moving groove is provided on the inner wall of the processing box 1, a second stopping block 353 is provided in the eighth moving groove, and a fifth connecting spring 163 is provided on the first stopping block 16. A ninth moving groove communicating with the eighth moving groove is provided on the side wall of the processing box 1, a third connecting plate 161 is pivotally connected to the sixth side wall of the first stopping block 1, and a fourth connecting plate 161 is pivotally connected to one end. Connection plate 16
2 is pivotally contacted, a push rod 171 is provided in the ninth moving groove, a third push plate 17 is provided at the top of the push rod 171 and a fourth push plate 172 is provided at the bottom thereof. When the lid plate 2 covers the processing box 1, the first stop block 16 is located in the eighth moving groove. The gas cylinder 26 drives the press plate 3 downward to the bottom of the first stop block 16, pushes the third push plate 17 downward, and moves the fourth push plate 172 downward in conjunction with the third push plate 17. 4th push plate 17
The third connection plate 161 and the fourth connection plate 162 are rotated around the connection point by 2. By rotating the third connection plate 161 and the fourth connection plate 162, the first stop block 16 is pushed and extended from the inside of the eighth moving groove. The gas cylinder 26 moves and drives the press plate 3 upward.
When the press plate 3 rises to the bottom of the first stop block 16, the fourth connection block 263 moves upward with respect to the press plate 3, and the first connection plate 2631 moves above the second connection plate 35. The press plate 3 is rotationally driven by the first motor, and the solution is stirred by the first stirring rod 31. When the heat reflux treatment of the solution is completed, the gas cylinder 26 makes the fourth connection block 263.
Is moved downward, and the fourth connection block 263 moves to the bottom of the second concave groove 303.
When the press plate 3 is subsequently rotationally driven by the first motor, the second connection plate 35 becomes the first connection plate 2.
Rotating to one side of 631, the first convex block 351 fits into the third concave groove, the second stop block 353 enters the seventh moving groove, and the first connecting plate 2631 and the second connecting plate 35 and 35 form a fixed connection, and the first connection rod 33 stands still at a predetermined position. When the press plate 3 is moved and driven downward by the gas cylinder 26, the solution is discharged from the drain port 1121.

A connection shaft 111 is provided in the first moving chamber 112, the connection shaft 111 penetrates the second connection block 19, and the second connection block 19 has a disk-shaped configuration. The sealing door 191 is rotatably connected to the inner wall of the first through chamber 190. A tenth moving groove is further provided on the inner wall of the first through chamber 190, a fifth connecting plate 192 is provided in the tenth moving groove, a sixth connecting spring 193 is provided on the fifth connecting plate 192, and a fifth connection is provided. A second sealing plate 194 and a second convex block 195 are provided on the plate 192, a second convex block 195 is provided on one side of the second sealing plate 194, and one end of the second first push block is a circle. It has an arc-shaped structure, and both the second sealing plate 194 and the second convex block 195 penetrate the inside of the first through chamber 190. When the heating and refluxing of the solution is completed, the press plate 3 is moved downward to the bottom of the processing box 1. The position of a corresponding number of second connection blocks 19 can be adjusted based on the amount of Dwarf lilyturf powder put in the processing box 1. When a predetermined number of second connection blocks 19 are rotated 180 degrees, the sealing door 191 wraps around in the first moving chamber 112, and the first push plate 32 moves upward, the first connecting rod 33 moves second. Extending from the groove 302, the first connecting rod 33 is inserted into the first through chamber 190, and the first connecting rod 33 and the second convex block 1 are inserted.
The 95 and the 95 are in contact with each other, and the second convex block 195 is pushed and moved into the tenth moving groove by the first connecting rod 33, and the second connecting plate 35 moves tenth in conjunction with the fifth connecting plate 192. It moves toward the inside of the groove, the solution in the processing box 1 enters the first through chamber 190 by the guide groove 330, and the sealing door 191 is pushed by the water flow and rotates into the first moving chamber 112 to enter. And the first
The solution in the moving chamber 112 is discharged from the drain port 1121.

A second penetration chamber is provided at the top of the first moving chamber 112, and a second connection block 19 is provided in the second penetration chamber.
A transmission roller 110 that cooperates with the transmission roller 110 is inserted, and the sixth push plate 110 is placed on the top of the transmission roller 110.
1 is provided, and a plurality of ring grooves are provided in the transmission roller 110. An equipment room is provided in the first connection block 11. A second motor 120 is provided in the equipment room, and a first transmission wheel 1201 that cooperates with the transmission roller 110 is provided on the output shaft of the second motor 120. An eleventh moving groove communicating with the second penetrating chamber is provided on the side wall of the second penetrating chamber, a second stopping rod 130 that cooperates with the ring groove is provided in the eleventh moving groove, and the second stopping rod 130 is provided. Second
A connecting rod 1301 is provided, and a return spring 1302 is provided on the second connecting rod 1301.
The second connecting rod 1301 extends from the inside of the eleventh moving groove. When the press plate 3 moves to the bottom of the processing box 1, the second connecting rod 1301 can be pushed and moved toward one end of the eleventh moving groove, and the second stop rod 130 moves and comes out of the ring groove. The sixth push plate 1101 can be pushed downward based on the position where the press plate 3 is located, and the transmission roller 110 is lowered to the position positioning position in conjunction with the sixth push plate 1101. The transmission roller 110 is rotationally driven by the second motor 120,
A predetermined number of second connection blocks 19 rotate in conjunction with the transmission roller 110, the second connection block 19 on one side of the press plate 3 rotates 180 degrees, and the first connection rod 33 rotates in the first penetration chamber. Inserted into 190, the solution at the top of the processing box 1 enters the first moving chamber 112 from the first connecting rod 33, and the solution is discharged from the drain port.

A twelfth moving groove is provided on the inner wall of the processing box 1, a moving ring 15 is provided in the twelfth moving groove, a first transmission chamber is provided on the side wall of the twelfth moving groove, and the moving ring 15 is provided in the first transmission chamber. A transmission shaft 150 that cooperates with each other is provided. A thirteenth moving groove is provided at the top of the twelfth moving groove, and the thirteenth moving groove is provided.
A third moving chamber is provided in the moving ring 15, a plurality of 14th moving grooves communicating with the third moving chamber are provided in the inner wall of the moving ring 15, and a seventh connection spring 154 is provided in the 14th moving groove. A second stirring rod 151 is provided at one end of the seventh connecting spring 154, a fifth push plate 152 that interacts with the thirteenth moving groove is inserted into the third moving chamber, and a fifth push plate 152 is inserted at the bottom of the fifth push plate 152. A support spring 153 is provided. A fifteenth moving groove communicating with the thirteenth moving groove is provided on the inner wall of the processing box 1, an eighth connecting spring 1401 is provided on the inner wall of the fifteenth moving groove, and a first push block 140 is provided at one end of the eighth connecting spring 1401. Is provided, and the first push block 140
Arc surfaces are provided at the top and bottom of each. When the lid plate 2 covers the processing box 1, the first motor and the transmission shaft 150 form a transmission cooperation. When the press plate 3 is in an ascending state, the press plate 3
While pushing the first push block 140, it is moved into the fifteenth moving groove. The air in the 15th moving groove enters the 13th moving groove and is moved downward while pushing the 5th push plate 152 by the atmospheric pressure, and the 14th movement while pushing the air in the 3rd moving chamber by the 5th push plate 152. Push it into the groove. The second stirring rod 151 extends from the inside of the 14th moving groove, and the press plate 3 is rotationally driven by the first motor, and at the same time, the transmission shaft 150 is rotationally driven. The moving ring 15 is rotated in conjunction with the transmission shaft 150. The first stirring rod 31 and the second stirring rod 151 cooperate to stir the solution. When the heating / reflux of the solution is completed, the press plate 3 is pushed downward by the gas cylinder 26 while being pushed, the press plate 3 is separated from one side of the first push block 140, and the first push block 140 is moved from the inside of the fifteenth moving groove. Extending, the second stirring rod 151 enters the 14th moving groove, and
The press plate 3 normally penetrates to the bottom of the processing box 1, and after the dwarf lilyturf powder is pushed to the bottom of the processing box 1, the solution in the upper layer is independently collected.

A second transmission chamber is provided on the side wall of the second penetration chamber, and a second transmission wheel 160 for transmitting between the transmission shaft and the press plate is provided in the second transmission chamber. By installing the second transmission wheel, the moving ring and the press plate are rotated in different directions. By installing the second stirring rod in an inclined manner, when the second stirring rod rotates in conjunction with the moving ring, a water flow that moves upward is generated in the processing box, and the water flow that moves upward and the first. The collision with the stirring rod can enhance the contact effect between the water stream and the dwarf lilyturf powder, and the dwarf lilyturf powder is more uniformly contacted with the solution.

A figurine plate 14 is provided at the bottom of the processing box, a discharge port is provided on the side wall of the processing box, an 18th moving groove is provided at the top of the discharge port, and a fourth sealing plate 12 is provided in the 18th moving groove. , A handle is provided on the fourth sealing plate. When the dwarf lilyturf powder is pushed downward by the press plate, the dwarf lilyturf powder is pushed onto the figurine plate. After the solution is drained from the drainage port, the 4th sealing plate can be opened, the press plate can be moved slightly upward, and the figurine plate can be taken out from the drainage port, which makes it possible to treat the dwarf lilyturf powder. Become. When the figurine plate is taken out, the brush may be taken out from the discharge port, and the dwarf lilyturf powder attached to the bottom surface of the press plate can be cleaned.

The sleeve 22 is fitted onto the reflux tube, a second cavity is provided on the side wall of the sleeve, a first sorting plate 221 is provided in the second cavity, and a notch is provided at the top of the first sorting plate.
As a result, both sides of the second cavity are communicated with each other. A water reservoir tank 25 is provided on the lid plate, a second sorting plate 255 is provided in the water reservoir tank, and the water reservoir tank is divided into an upper chamber and a lower chamber by the second sorting plate. A first water pipe 256 to communicate with is provided, and a second water pipe 257 to communicate with the second cavity is provided on the side wall of the upper chamber. A sealing film 251 is provided on the inner wall of the lower chamber, and the sealing film is made of rubber. A second push block 252 is further provided in the lower chamber, a third convex block 2521 is provided in the second push block, and a second
The sorting plate is provided with a second through groove, the upper chamber is provided with a connection frame 254, the connection frame is provided with a baffle 253, the second sorting plate is provided with a 19th moving groove, and the baffle is provided with a fifth connection block 2531. Is provided, and the second push block is provided with a connection groove that cooperates with the fifth connection block. The first motor is a double-head type motor, and the third transmission wheel 23 is provided on the other output shaft of the first motor, and the fourth transmission wheel 24 that cooperates with the third transmission wheel on the lid plate.
Is provided. 4th convex block 24 that cooperates with the 3rd convex block on the 4th transmission wheel
1 is provided. When the first motor operates, the fourth transmission wheel rotates together with the third transmission block, and the fourth convex block intermittently contacts the third convex block. The third convex block is moved forward by the fourth convex block, and the second push block is moved downward into the room. The baffle moves with the movement of the second push block. When the baffle moves to the top of the second through groove, the second push block presses the water flow in the lower chamber, and the water flow in the lower chamber becomes the first.
It enters the second cavity through the water pipe, and the water flow in the second cavity enters the upper chamber through the second water pipe. If the 4th convex block is not in contact with the 3rd convex block, the second
The push block moves back and forth, the baffle separates from the top of the second through groove, the water flow in the upper chamber falls from the second through groove, and the water flow flows into the lower chamber again. By constantly exchanging the water in the second cavity with the rotation of the fourth transmission wheel, the gas that has entered the reflux tube can be cooled by the water flow, and the gas is rapidly liquefied and then refluxed into the processing box. do. It also lowers the altitude of the perfusion tube and reduces the volume of the processing box.

The positioning block 13 is provided on the top of the processing box, and the lid plate is provided with a positioning groove that cooperates with the positioning block. By mutual fitting of the positioning block and the positioning groove,
It is possible to guarantee the transmission cooperation between the first motor and the transmission shaft when the lid plate covers the processing box.

The drawings of the present application are schematic views, and the specific size thereof is based on the actual implementation.

Claims (4)

It is a method of distinguishing the origin of Dwarf lilyturf,
a) A step of obtaining a filtrate a when Dwarf lilyturf powder and an aqueous ethanol solution having a volume percent concentration of 85% to 95% are placed in a treatment box, heated under reflux and completely extracted.
b) A step of concentrating the filtrate a to non-ethanol, adding ionized water and gently precipitating at 2 to 10 ° C., picking up the supernatant and filtering to obtain the filtrate b.
c) Porous polymer bead column chromatography was performed on the filtrate b, and sequentially deionized water, an aqueous ethanol solution having a volume percent concentration of 15% to 25%, an aqueous solution of 25% to 35% ethanol having a volume percent concentration, and a volume percent concentration. The process of collecting the effluent by performing gradient elution using a 35% to 45% aqueous ethanol solution, and
d) A step of measuring the content of offiopogonin D in Dwarf lilyturf powder by high performance liquid chromatography, and
e) Dragon brain glycoside (l-borneol 2-O-β-D) in the effluent in step c
-Apioflanosyl- (1 → 6) -β-D-glucopyranoside) is inspected, and the content of offiopogonin D in Zhejiang dwarf lilyturf having the same mass as the content of detected offiopogonin D in step d. If the test dwarf lilyturf contains dragon brain glycoside, it is judged that the test dwarf lilyturf contains a component of Zhejiang dwarf lilyturf, and the content of offiopogonin D in the test dwarf lilyturf is the same mass. If the content of Ofiopogonin D in Zhejiang Dwarf lilyturf is higher than that of Dwarf lilyturf, it includes the step of determining that Sichuan Dwarf lilyturf is mixed in the test Dwarf lilyturf.
The bed volume of the porous polymer beads in step c is 10 to 20% of the volume of the filtrate b.
Gradient elution in step c is carried out sequentially using deionized water, an ethanol aqueous solution having a volume percent concentration of 20%, an ethanol aqueous solution having a volume percent concentration of 30%, and an ethanol aqueous solution having a volume percent concentration of 40%.
A lid plate is provided in the processing box in step a, a first concave groove is provided in the inner wall of the processing box, a heating block is provided in the first concave groove, a return tube is provided in the lid plate, and the lid plate is further provided. A gas cylinder is provided, a press plate that interacts with the processing box is provided on the piston rod of the gas cylinder, a plurality of first through holes are provided in the press plate, and the press plate is rotationally driven on the side wall of the processing box. A first motor is provided, a first connection block is provided on the side wall of the processing box, a first moving chamber is provided in the first connecting block, and a plurality of press plates are provided in the first moving chamber. A second connection block that cooperates is provided, a drainage port is provided on the side wall opposite to the lid plate of the first moving chamber, a launch port is provided on the lid plate, and the opposite side of the launch port to the press plate. A first sealing plate is provided in the box, a supply port is provided on the side wall of the processing box, a third sealing plate is provided in the supply port, and a press plate is used as a lid plate by a gas cylinder to obtain a mixed solution. When the press plate moves toward one side near the lid plate of the supply port, the Janohige powder is put into the processing box through the supply port, and after the Janohige powder is put in, the ethanol aqueous solution is launched. When the addition of the Janohige powder and the ethanol aqueous solution is completed, the mixed solution in the processing box is heated by the heating block, the press plate is rotationally driven by the first motor, and the mixed solution is driven by the press plate. After stirring, the steam enters the recirculation tube, liquefies, drops into the processing box, and when the heating recirculation is completed, the press plate is pushed by the gas cylinder and moved to the opposite side of the lid plate. While pushing the powder, move it downward, the Janohige powder is pressed to the bottom of the processing box, the second connection block and the press plate cooperate, and the mixed liquid on the side closer to the lid plate of the press plate moves first. Entering the room, the solution is discharged from the drain port, and the filtrate a can be obtained.
How to identify the origin of Dwarf lilyturf. A first moving groove is provided on the bottom of the press plate on the opposite side of the lid plate, a first stirring rod is provided in the first moving groove, and a first sliding block is provided on the side wall of the first stirring rod. 1. The method for identifying a dwarf lilyturf producing area according to claim 1, wherein a first sliding groove that cooperates with the first sliding block is provided on the inner wall of the moving groove. The press plate is provided with a second moving groove, a first connecting rod is provided in the second moving groove, a first through groove is provided on one side of the second moving groove near the lid plate, and the first connecting rod is provided. A guide groove that cooperates with the first through groove is provided, a first through chamber that cooperates with the first connection rod is provided in the second connection block, and the first connection is provided on the opposite side of the press plate from the lid plate. The method for identifying a dwarf lilyturf producing area according to claim 2, wherein a first push plate is provided which moves the rod toward the outside of the second moving groove while pushing the rod. The press plate is moved and driven upward by the gas cylinder, the press plate is located above the supply port, the first stirring rod extends from the inside of the first moving groove, and the press plate is rotationally driven by the first motor. 1 The stirring rod stirs the solution in the processing box with the rotation of the press plate, the solution heats and recirculates in the processing box, and when the heating and recirculation of the solution is completed, the press plate is rotationally driven by the first motor. When the first connecting rod rotates to the position corresponding to the second connecting block, the gas cylinder moves and drives the press plate to the opposite side of the lid plate, and the press plate moves toward the opposite side of the lid plate to the processing box. When moving to the bottom of the, the first connecting rod moves to one side of the second connecting block, the first pushing plate and the first stirring rod are pressed onto the Janohige powder, and the first stirring rod is inside the first moving groove. The first push plate moves toward the press plate, the first connecting rod extends from the inside of the second moving groove, and the first connecting rod becomes the first.
Inserted into the through chamber, the solution on one side of the press plate near the lid plate enters the guide groove from the first through groove, the water flow enters the first moving chamber along the guide groove, and the extract is drained. The method for distinguishing the production area of Dwarf lilyturf according to claim 3, wherein the powder of Dwarf lilyturf flows out from the mouth and stays in the processing box to obtain the solution a independently.

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