JP6948646B2 - Agents or foods that have the effect of improving vascular endothelial function - Google Patents

Description

The present invention relates to an agent capable of improving vascular endothelial function and used for prevention or treatment of cardiovascular diseases such as hypertension and arteriosclerosis, and foods and drinks to which these functions are imparted.

Cardiovascular disease (CVD) is the leading cause of death in the United States and accounts for 32% of all deaths. In Japan, CVD has been shown to be the second leading cause of death based on national medical expenditure data and is recognized as a common disease. In recent years, a variety of treatments have been provided to people with short-term risk factors for CVD, such as smoking, hypertension, and high cholesterol, along with activities to alert them to risk. As a result, the number of people with high CVD risk factors is gradually decreasing. However, even for healthy people with low risk factors related to CVD, lifelong CVD risk countermeasures are necessary. Therefore, it is important to reduce the lifetime risk of developing CVD in a daily diet.
To date, several studies have reported functional foods for the treatment or prevention of hypertension, arteriosclerosis and ring arterial disease. For example, Patent Document 1 discloses an arteriosclerosis preventive treatment agent, a NO action enhancer, or a vascular endothelial function improving agent containing at least one kind of chlorogenic acid, caffeic acid, or ferulic acid. Patent Document 2 describes an agent for improving vascular endothelial function using an onion or a processed onion product. In addition, intake of omega 3 fatty acids such as docosahexaenoic acid and eicosapentaenoic acid has been reported as a functional component having an effect of preventing cardiovascular diseases and improving vascular endothelial function (Non-Patent Documents 1 to 3).

As described in the above-mentioned literature, it is considered effective to improve the vascular endothelial function as a countermeasure against CVD risk, and the CVD risk is estimated by quantitative measurement of the vascular endothelial function. As a non-invasive method for examining vascular endothelial function, measurement of blood flow-mediated vasodilation (FMD), which is an arterial dilation reaction to shear stress based on changes in blood flow, is used in clinical medicine (FMD). Non-Patent Documents 4 and 5).
Vascular endothelial cells produce nitric oxide (NO) in response to increased shear stress from the bloodstream. This nitric oxide (NO) acts on smooth muscle to dilate the blood vessel diameter. FMD is measured by using this response as an index. First, the cuff wrapped around the upper arm is inflated to stop blood flow, and then the cuff is contracted to release and increase blood flow. Induces FMD with. The FMD-induced dilated response of the vessel diameter can be detected as an image by diagnostic ultrasound imaging. Currently, FMD measurements are used to diagnose the early signs of hypertension and arteriosclerosis. Therefore, as a method for evaluating the preventive action and effect of functional foods on hypertension and arteriosclerosis, it is effective to measure the vascular endothelial responsiveness by FMD induction using the effect of improving vascular endothelial function as an index. Is used as a target.

Japanese Unexamined Patent Publication No. 2003-261444 Japanese Unexamined Patent Publication No. 2013-53084

Adkins Y. , Journal of Nutritional Biochemistry, (2010) 21,781-792. Kromhout D. , European Heart Journal, (2012) 33, 436-443. Wang Q. , Atherosclerosis, (2012) 221,536-543. Charakida M. , Et al. , European Heart Journal, (2010) 31,284-2861. Kelm M. , American Journal of Physiology Heart and Circuity Physiology, (2002) 282, 1-5.

An object of the present invention is to provide a supplement or health food having a function for treating or preventing hypertension and arteriosclerosis, which are easy and highly effective. In particular, it is an object of the present invention to provide an agent or food having an action of improving vascular endothelial function for reducing the risk of CVD such as hypertension and arteriosclerosis.

The present inventors have found that ingestion of sachainchi oil improves vascular endothelial function. That is, it was found that the vasodilatory function induced by blood flow-dependent vasodilation (FMD) was remarkably enhanced by ingesting Sachainch oil, and from this, the responsive function of the vascular endothelium of Sachainch oil was enhanced. Based on the knowledge about new uses based on the action and effect to be improved, the following inventions could be made.
[1] An agent containing sacha inch oil as an active ingredient and having an action of improving vascular endothelial function.
[2] The agent according to the above [1], which has an action of improving the contraction / expansion function of the vascular endothelium.
[3] The agent according to the above [1] or [2], which comprises taking 100 mg to 1,000 mg of sacha inch oil at a time.
[4] A food containing sacha inch oil and having an action of improving vascular endothelial function.
[5] A food having an action of improving the vascular endothelial function in the form of taking 100 mg to 1,000 mg of Sacha Inch oil at a time.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide supplements and health foods having a function for treating or preventing hypertension and arteriosclerosis, which are easy and highly effective. In particular, it is possible to provide an agent or food having an action of improving vascular endothelial function such as contraction / expansion function of vascular endothelium for reducing the risk of leading to CVD such as hypertension and arteriosclerosis.

A: It is a figure which shows the typical record of the arterial diameter (AD) dilation response by the FMD response in the subject taking Sachainch oil. B: Calculation of FMD expansion rate (FMD%). It is a graph which shows the baseline in the test example of this application, and the FMD expansion rate (FMD%) of the subject who took placebo (canola oil) or sacha inch oil.

Sacha inchi oil is an oil extracted from the general name of the Todaigusa family, which originates in Peru, South America: "Sacha inchi" (scientific name: "Pulkenetia", (Prukenetia)). Inca green nut oil, Amazon green nut oil, green nut oil, INCI name: Sometimes called "Pulkenetia borbilis seed oil". The INCI name is a display name specified by the Japan Cosmetic Industry Association.

Examples of the sacha inch include Plukenetia volubilis L., Plukenetia huayllabambambana RW Bussmann, C. Telelez & A. Glenn, and the like. These may be used alone or in combination of two or more. Of these, Plukenetia volubilis L. is preferred.

It is known that the nutritional components of sachainchi oil include calories, proteins, lipids, carbohydrates, sodium, cholesterol, vitamin E, and α-linolenic acid per 100 g of the nutritional components.
The calorific value is preferably an oil component having 800 kcal to 1,000 kcal per 100 g of the nutritional component, and more preferably 850 kcal to 950 kcal.
The protein content is preferably an oil component having 0.01 g to 0.5 g per 100 g of the nutritional component, and more preferably 0.05 g to 0.2 g.
The lipid content is preferably an oil component having 10 g to 200 g per 100 g of the nutritional component, and more preferably 50 g to 150 g.
The carbohydrate content is preferably an oil component having 0 g to 10 g per 100 g of the nutritional component, and more preferably 0 g to 2 g.
The sodium content is preferably 0 mg to 10 mg per 100 g of the nutritional component, more preferably 0 mg to 2 mg.
The cholesterol content is preferably 0 g to 10 g per 100 g of nutritional component, and more preferably 0 g to 2 g.
The content of vitamin E is preferably 100 mg to 300 mg per 100 g of nutritional component, more preferably 150 mg to 250 mg.
The content of α-linolenic acid (Omega3) is preferably 30% to 70%, more preferably 40% to 60%, per 100 g of the nutritional component.

Examples of the quality standard of sacha inch oil include acid value, peroxide value, iodine value and the like.
The acid value is preferably 3 mgKOH / g or less, more preferably 1 mgKOH / g or less, in the case of so-called raw squeezed oil squeezed by cold pressing or the like. When the acid value exceeds 3 mgKOH / g, the amount of free fatty acids in the sachainchi oil increases, and the sachainchi oil tends to be oxidized. The acid value can be determined by titrating the free fatty acid contained in 1 g of fat or oil with potassium hydroxide. For example, a test paper for measuring acid value (trade name: "Fat and oil deterioration degree judgment test paper AV-CHECK"). , J-Oil Mills Co., Ltd.) can be used for simple measurement. The acid value represents the amount of free fatty acid contained in Sacha inchi oil.

The peroxide value is preferably 30 meq / kg or less, more preferably 10 meq / kg or less, and particularly preferably 5 meq / kg or less. When the peroxide value exceeds 30 meq / kg, an offensive odor is generated in the food, the flavor, color tone and the like are changed, and the nutritional components are decomposed, which may not only affect the taste but also have a harmful effect on the human body. Examples of the method for measuring the peroxide value include a sodium thiosulfate titration method in which potassium iodide is allowed to act on oxidized fats and oils to obtain free iodine by a titration method. For example, the peroxide value is measured. Measurement can be easily performed using a measurement test paper (trade name: "POV test paper", manufactured by Shibata Chemical Co., Ltd.). The peroxide value represents the amount of lipid peroxide produced by the oxidation of sacha inch oil by oxygen in the air, and represents the degree of oxidation of sacha inch oil.

The iodine value is an index of the degree of unsaturation of fats and oils, but in the case of sacha inchi oil having a large content of α-linolenic acid (Omega3), it is an index showing the content of unsaturated fatty acids and the degree of deterioration thereof. As a method for measuring the iodine value, after adding excess iodine monochloride to fats and oils and reacting them, unreacted iodine monochloride (ICl) is decomposed with potassium iodide (KI) to produce free iodine (I _2). ) Is titrated with a 0.1 N sodium thiosulfate (Na ₂ S ₂ O _{3 ) solution, and the amount of iodine (I 2} ) corresponding to the amount of absorbed iodine monochloride (ICl) is converted to 100 g of fats and oils. Examples thereof include the Weiss-cyclohexane method, which is obtained by calculating the weight (g) of iodine to be absorbed.

The iodine value is preferably 130 mgI / 100 mg or more, and more preferably 190 mgI / 100 mg or more. On the contrary, if it is less than 130 mgI / 100 mg, the unsaturated fatty acid content itself may be low, or the unsaturated fatty acid may have been oxidized, and the quality is combined with the acid value, peroxide value, and fatty acid composition measurement value. Deterioration needs to be verified.

The site of the sacha inch used as the extraction raw material in the present invention is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include a seed portion, and among these, the endosperm portion is used from the viewpoint of extraction efficiency. More preferred.

The extraction method of sacha inch oil is not particularly limited and may be appropriately selected depending on the purpose. For example, seeds are taken out from ripe fruits, washed and disinfected, peeled, and then crushed by a crusher. To get. Sacha inchi oil can be obtained by applying pressure to the obtained crushed product with a squeezing machine to squeeze the extract, passing the extract through a filter, and filtering the extract.

Examples of the method for extracting the extract by the squeezing machine include a cold press method and a hot press method. Among these, the cold press method is preferable from the viewpoint of preventing deterioration due to heat. The cold press method is a method of squeezing oil by applying pressure without applying heat. The temperature in the cold press method is not particularly limited and may be appropriately selected depending on the intended purpose. For example, 0 ° C. to 80 ° C. is preferable, and 5 ° C. to 40 ° C. is more preferable.

The sacha inch oil is not particularly limited and may be appropriately selected depending on the intended purpose, and a commercially available product may be used. Examples of the commercially available product include product name: "Inca Green Nut Inca Inchi Oil" (manufactured by Specified Nonprofit Corporation Alcoylis), Product Name: "Pacha Mama Premium Sacha Inchi Oil" (manufactured by Pacha Mama Co., Ltd.), Product Name. : "Agricultural University Sacha Inchi Oil" (manufactured by Mercado Tokyo Agricultural University) can be mentioned. These may be used alone or in combination of two or more.

The purity of the sacha inch oil is preferably 80% to 100%, more preferably 90% to 100%, and particularly preferably 95% to 100%.

The dosage form of the agent having an action of improving the vascular endothelial function in the present invention is not particularly limited and may be appropriately selected depending on the intended purpose. For example, a liquid agent, a hard capsule agent, a soft capsule agent, a granule, a tablet, etc. Examples include syrups and lozenges. Among these, liquid preparations, hard capsules, soft capsules, granules, tablets, syrups, and lozenges, which are dosage forms that can be taken orally, are preferable from the viewpoint of convenience of use, and liquid preparations, granules, tablets, and hard capsules. Agents, soft capsules and syrups are more preferred.

The content of the sacha inch oil varies depending on the dosage form.
The content of the sacha inch oil in the case of a liquid preparation obtained by bottling the sacha inch oil as it is is preferably 30% by mass to 100% by mass with respect to the total amount of the agent for improving the vascular endothelial function.
The content of sacha inch oil in the hard capsule or soft capsule is preferably 30% by mass to 95% by mass with respect to the total amount of the agent.
The content of sacha inch oil in the syrup agent is preferably 30% by mass to 100% by mass with respect to the total amount of the agent.
The content of sacha inch oil in the granules is preferably 30% by mass to 70% by mass with respect to the total amount of the agent.
The content of sacha inch oil in the tablet is preferably 10% by mass to 50% by mass with respect to the total amount of the agent.
In the above-mentioned dosage form, a preparation having an excessively low content of sacha inch oil is not suitable because the intake is high.

The agent having an action of improving the vascular endothelial function in the present invention is essential to contain sachainchi oil as an active ingredient, and further enhances the effect, imparts an additional effect, or is a preparation. Other components necessary for preparing the shape may be appropriately selected and contained as necessary.
Examples of the other components include auxiliary raw materials used in producing an agent for improving vascular endothelial function, additives usually used in a formulation operation, and the like.

The auxiliary raw material or the additive is not particularly limited and may be appropriately selected depending on the intended purpose. For example, unsaturated fatty acids such as α-linolenic acid, γ-linolenic acid, linoleic acid, oleic acid, leukotriene, prostaglandin, thromboxane, eicosapentaenoic acid, docosahexaenoic acid; glucose, fructose, sucrose, maltose, sorbitol, etc. Stebioside, rubusoside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbinate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester , Sucrose fatty acid ester, sorbitan fatty acid ester, arabic gum, carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, preservatives, etc. Be done. These may be used alone or in combination of two or more.

As a method of using the agent having an action of improving the vascular endothelial function containing sacha inch oil according to the present invention, it is preferable to ingest 0.1 g to 3 g of sacha inch oil per dose. Preferably, as sacha inch oil, 0.1 g to 1 g (100 mg to 1,000 mg) / dose is taken once. The above-mentioned intake may be taken daily or may be taken as a single dose.
The agent having an action of improving the vascular endothelial function in the present invention is intended to be used as a supplement preparation classified as a health food or a health functional food, or as a preparation classified as a quasi-drug or a pharmaceutical product.

The food containing the sacha inchi oil of the present invention having an action of improving the vascular endothelial function includes sacha inchi oil, and further contains other ingredients appropriately selected as necessary. It means a wide range of foods such as general foods that are taken orally, or supplements called health foods and health functional foods.
The food may be a food containing sacha inchi oil so as not to interfere with its activity, or may be a dietary supplement containing sacha inchi oil as a main component.

The food is not particularly limited and may be appropriately selected according to the purpose. For example, carbonated beverages, fruit / fruit juice beverages, coffee beverages, tea-based beverages, tea beverages (green tea, Karyu tea, tea, etc.), Beverages such as soy milk, vegetable drinks, sports drinks, dairy drinks, soft drinks, dairy drinks, lactic acid bacteria drinks; cold confectionery such as ice cream, ice sherbet, shaved ice; Noodles such as noodles and instant noodles; candy, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bread and other confectionery; crab, salmon, asari, tuna, sardine, shrimp, Marine products such as bonito, mackerel, whale, oyster, saury, squid, red mussel, scallop, abalone, sea urchin, squid, and tokobushi; marine and livestock processed foods such as kamaboko, ham, and sausage; Fats and oils such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, and processed fats and oils; seasonings such as sauces and sauces; , Una bowl, Hayashi rice, Oden, Marbodorf, Beef bowl, Meat sauce, Egg soup, Omuraisu, Dumplings, Shumai, Hamburg, Meat balls and other retort pouch foods; And so on.
In the case of these food aspects, it is possible to produce a food having an action of improving the vascular endothelial function according to the present invention by producing the food by containing sacha inch oil.

The amount of sacha inch oil added to the food varies depending on the type of the target food and cannot be unconditionally specified, but the content is such that 0.1 g to 3 g of sacha inch oil can be ingested per meal. It is preferable to do so. Preferably, the sacha inch oil is 0.1 g to 1 g (100 mg to 1,000 mg) / serving for one meal.
The food can be taken orally on a daily basis, and the vascular endothelial function can be improved extremely effectively by the action of sacha inch oil, which is an active ingredient.

The action effect of improving the vascular endothelial function in the present invention means having an action of improving the contraction / expansion function of the vascular endothelium. That is, it means that it has an action of preventing or ameliorating a circulatory system disease, particularly a disease or a symptom caused by a decrease in vascular endothelial function due to arteriosclerosis, a decrease in vascular elasticity, or the like. The vascular endothelial cells have an action of expanding the blood vessel diameter in response to nitric oxide (NO) produced in response to shear stress due to an increase or decrease in blood flow. The measurement of blood flow-mediated vasodilation (FMD) reaction is interpreted as measuring the so-called flexibility and suppleness of blood vessels, and is generally used as a non-invasive evaluation method for vascular endothelial function. It is used. The action of improving the vascular endothelial function means the action of improving the function of contracting / expanding the blood vessel diameter in response to signals of various physiological environments, and the action of maintaining or restoring a flexible and supple blood vessel. Means.
The degree of improvement in vascular endothelial function can be quantitatively evaluated as the rate of increase in blood vessel diameter (FMD value (%)). That is, the larger the FMD value, the higher the responsive function of the blood vessel, and it is evaluated as a so-called highly flexible blood vessel, and the lower the FMD value, the lower the flexibility. It is known that the FMD value (%) correlates with the risk of developing CVD. That is, the FMD value (%) of a healthy person is about 6 or more, and the FMD value (%) of patients with vascular diseases such as arteriosclerosis and those who are at risk of it is lower than that, and is generally less than 4. Is said to be.
In the present invention, the action of improving or improving the vascular endothelial function shall be used for a healthy person, a patient with a vascular disease such as arteriosclerosis, and a person who may have the same. For example, when the FMD value of a healthy person increases, it can be determined that the vascular endothelial function of that person has improved. In addition, when the FMD value of a patient with a vascular disease such as arteriosclerosis and a person who may have an increase in the FMD value, it can be judged that the vascular endothelial function of the person has been improved.

Endothelial function has been shown to be closely associated with the risk of developing hypertension and cardiovascular disease. If the vascular endothelial function continues to decline, arteriosclerosis progresses and hypertension is likely to occur. On the contrary, if the vascular endothelial function can be enhanced, arteriosclerosis can be prevented and hypertension is less likely to occur. Therefore, improvement of vascular endothelial function is expected to lead to improvement or prevention of hypertension.
The vascular endothelial function improving agent of the present invention is used for prevention or treatment of diseases such as arteriosclerosis, hypertension, abnormal lipid metabolism, diabetes, chronic kidney disease, obesity / metabolic syndrome, coronary artery disease, and cerebrovascular disease. Can be done. The vascular endothelial function improving agent of the present invention is effective not only for diabetic patients and subjects who are prone to obesity, but also to healthy subjects.

Hereinafter, the present invention will be further described with reference to Examples. However, the present invention is not limited to these examples. As an example, a test example demonstrating the effect of improving vascular endothelial function by taking Sachainch oil in healthy volunteers is presented.
1-1. Test material Sacha inch seeds from Peru were cold pressed to extract oil and allowed to stand for 24 hours before removing the precipitate. The supernatant was used as test sacha inch oil. For Sacha Inch oil, 350 mg of soft capsules were prepared with gelatin colored with caramel color.
Using canola oil as the target agent (placebo), 350 mg of soft capsules were prepared in the same manner.

1-2. Composition analysis of test oil The fatty acid composition of canola oil and canola oil used in the test was analyzed by a gas chromatography method according to the following procedure.
An oil sample (40-60 mg) was dissolved in hexane (2 mL) with the internal standard heptadecanoic acid (6 mg) in a screw capped test tube. After blowing nitrogen gas to evaporate the hexane, the residue was dissolved in 0.5 M NaCl / methanol, then purged with nitrogen gas and then sealed. The solution was heated at 100 ° C. for 9 minutes with shaking. After ice-cooling, boron trifluoride-methanol reagent was added to the sample, purged with nitrogen gas, and then sealed. The sample was heated at 100 ° C. for 7 minutes, shaken and then iced. The sample was then mixed with hexane (3 mL) and saturated saline (5 mL) was added.
The mixture was centrifuged at 1,500 rpm for 10 minutes and the hexane layer was recovered as a gas chromatography (GC) analytical sample using flame ionization detection (FID).
For GC, an InertCap Pure-WAX column, 30 m × 0.25 mm × 0.25 μm (GL Sciences, Tokyo, Japan) was used, and the carrier gas was helium at 100 kPa. The injector and detector temperatures were 250 ° C and 260 ° C, respectively. The oven temperature was maintained at 150 ° C. for 5 minutes, then raised to 250 ° C. at a rate of 10 ° C./min and the temperature was maintained for 20 minutes.
The fatty acid compositions of canola oil and canola oil used in the examples are shown in Table 1.

2. Subjects Twenty healthy students (10 males and 10 females) aged 19 to 21 were recruited at Tokyo University of Agriculture. Healthy subjects with no history of hypertension, diabetes, or dyslipidemia were selected to avoid potential effects of vascular endothelial function. The research protocol was approved by the Tokyo University of Agriculture Human Research Ethics Committee. All subjects provided informed consent to participate in the study.

3. 3. Test method In order to evaluate the ability of Sacha Inch oil to improve vascular endothelial function, FMD measurement under food restriction was performed. Subjects received a single random dose of soft capsules of canola oil or canola oil in a crossover manner. The second dose was given one week later.
Subjects should consume 90-130 g of cereal / meal (Frugra®, Calbee) and 600-800 mL / day of milk the day before and the morning of the test, and should not consume any other dietary supplements. bottom.

3-1. FMD measurement and blood pressure measurement The subjects took soft capsules containing 350 mg of canola oil or canola oil, and FMD was measured 3 hours after the administration. Prior to measuring FMD, the subject's blood pressure was measured using an electronic sphygmomanometer (ES-P2000, Terumo).
For FMD measurement, pressure (blood pressure + 40 mmHg) was applied to the forearm for 5 minutes using a mercury sphygmomanometer cuff to stop blood flow in the brachial artery. The pressure was then released by contracting the cuff and the brachial artery was simultaneously monitored by an ultrasonic diagnostic device (LOGIQ P6, GE Healthcare). Arterial diameter (AD) was measured using FMDscape software (Mediacross Co., Ltd.). The coefficient of expansion of FMD (% FMD) was calculated based on the arterial diameter (AD) measurement.
FIG. 1 shows a typical record of arterial diameter (AD) dilation response due to FMD response in subjects taking Sachainch oil (FIG. 1A). Subject's FMD response reached a maximum 60 seconds after cuff release. Based on the maximum arterial diameter (AD max) and baseline arterial diameter (AD at rest), the FMD swelling rate (% FMD) was calculated as (AD max − AD at rest) / AD at rest (FIG. 1-2). ..

3-2. Blood Tests Subjects with high-density lipoprotein cholesterol (HDL) and low-density lipoprotein cholesterol (LDL) levels after taking soft capsules containing 350 mg of sachainchi oil or canola oil were measured by Alere Afinion AS100 Analyzer (Alere Inc.). ) Was used for measurement.

3-3. Statistical processing Analytical values are expressed as mean ± standard deviation. Differences in mean values were evaluated by one-way ANOVA followed by Bonferroni multiple comparison test. p <0.01 was considered to be statistically significant.

4. Test Results Table 2 shows the characteristics of the subjects, blood pressure after taking Sachainch oil (Example) and canola oil (control drug), high-density lipoprotein cholesterol (HDL) level, and low-density lipoprotein cholesterol (LDL) level. By using a restricted diet, the effect of diet on vascular endothelial function was prevented.
Taking Sacha Inch oil did not affect blood pressure. In addition, regarding the blood cholesterol level, no effect was observed on the HDL value and the LDL value of taking canola oil as compared with the target agent, canola oil.

4-1. FDM test results The FMD expansion rate (% FMD) in the FMD response of the subjects stratified by gender is shown in Table 3 and FIG.

The subjects' FMD expansion rates at baseline were 9.7 ± 2.9% (male) and 12.4 ± 1.9% (female), totaling 11.2 ± 2.5% (total). .. Normal vascular endothelial function is known to have an FMD expansion rate of 6% or more (Ozkayar et. Al., Nephrology (2015) 35, 72-79). Therefore, it was confirmed that all the subjects in this study had normal vascular endothelial function. Those who took Sachainch oil had an FMD expansion rate of 17.3 ± 1.5% (male) and 17.1 ± 2.1% (female), and the overall rate was 17.2 ± 1.8% (17.2 ± 1.8%). Total) and significantly increased. On the other hand, the FMD expansion rate of those who took canola oil, which is the target agent (placebo), was 11.6 ± 2.3% (male) and 11.9 ± 1.8% (female). As 11.8 ± 2.1% (total), no significant increase was induced.

The vascular endothelium is a tissue that maintains vasomotor nervous system tone in response to shear stress caused by changes in blood flow and blood pressure. The above test results showed that taking Sachainch oil improved the vasodilatory capacity of the arterial diameter in normal vascular endothelial function. The fact that taking Sachainch oil did not affect blood pressure suggests that the vasodilatory effect is not due to an increase in blood flow, but to an effect of improving responsiveness to changes in blood flow. Therefore, it was shown that taking Sachainch oil has the effect of improving vascular endothelial function. In particular, it was shown that the contraction / expansion function of the vascular endothelium can be improved.
Endothelial dysfunction can lead to various diseases of the circulatory system such as hypertension and arteriosclerosis. Therefore, improving the vascular endothelial function having a vascular diameter adjusting function in response to changes in blood flow may reduce the risk of cardiovascular disease. That is, the vascular endothelial function can be improved by taking Sachainch oil, and it can contribute to the prevention of cardiovascular diseases such as hypertension and arteriosclerosis.

Claims (4)

An agent for improving vascular endothelial function, in which Sacha inchi oil is used as an active ingredient and 100 mg to 1,000 mg of Sacha inchi oil is taken at a time. The agent according to claim 1, for improving the contraction / expansion function of the vascular endothelium. A food containing sachainchi oil and for improving vascular endothelial function in the form of taking sachainchi oil at a dose of 100 mg to 1,000 mg at a time . The food product according to claim 3, for improving the contraction / expansion function of the vascular endothelium.

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