JP6913454B2 - 揮発性有機塩素化合物の浄化方法 - Google Patents
揮発性有機塩素化合物の浄化方法 Download PDFInfo
- Publication number
- JP6913454B2 JP6913454B2 JP2016232127A JP2016232127A JP6913454B2 JP 6913454 B2 JP6913454 B2 JP 6913454B2 JP 2016232127 A JP2016232127 A JP 2016232127A JP 2016232127 A JP2016232127 A JP 2016232127A JP 6913454 B2 JP6913454 B2 JP 6913454B2
- Authority
- JP
- Japan
- Prior art keywords
- hydrogen peroxide
- rhodococcus
- groundwater
- strain
- oxygen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Description
[1]カタラーゼ生成能と、酸素の存在下で揮発性有機塩素化合物を分解する能力とを有する細菌を過酸化水素の存在下で揮発性有機塩素化合物に作用させて、当該揮発性有機塩素化合物を分解する分解工程を含む、揮発性有機塩素化合物の浄化方法。
[2]前記揮発性有機塩素化合物がトリクロロエチレン及び/又はシス-1,2-ジクロロエチレンである、[1]に記載の方法。
[3]前記細菌がロドコッカス(Rhodococcus)属の細菌である、[1]又は[2]に記載の方法。
[4]前記ロドコッカス(Rhodococcus)属の細菌がロドコッカス・ジョスティ(Rhodococcus jostii)RHA1株である、[3]に記載の方法。
[5]前記揮発性有機塩素化合物が地下水に含まれており、当該地下水を原位置で浄化する、[1]〜[4]のいずれかに記載の方法。
[6]前記過酸化水素が、前記地下水に導入された後3日以内に消失して酸素に変換される、[5]に記載の方法。
[7]前記過酸化水素を、前記地下水中の濃度が0.02%以下になるように導入し、ロドコッカス・ジョスティ(Rhodococcus jostii)RHA1株を、前記地下水中の菌数が5.0×108cells/ml以上になるように導入する、[5]又は[6]に記載の方法。
本試験では、RHA1株の過酸化水素に対する耐性を評価するために、過酸化水素濃度を段階的に変化させたコハク酸培地においてRHA1株の増殖状況を確認した。
本試験では、RHA1株のトリクロロエチレン分解能に対する過酸化水素の影響を評価するために、過酸化水素濃度を段階的に変化させたコハク酸培地においてトリクロロエチレンの分解試験を行った。
本試験では実汚染地下水中において、過酸化水素がRHA1株から生成されるカタラーゼによって分解されるかを確認するためにRHA1株と市販のカタラーゼ試薬を用いて試験を行った。
本試験では、実汚染サイトの地下水中においてRHA1株と過酸化水素を同時に導入して、過酸化水素の分解に伴う酸素供給によりシス-1,2-ジクロロエチレン(cis-DCE)が分解するか検討を行った。
本試験では、実汚染サイトの帯水層(土壌と地下水が存在する模擬環境)においてRHA1株と過酸化水素を添加して、cis-DCEが分解するか検討を行った。
Claims (1)
- カタラーゼ生成能と、酸素の存在下で揮発性有機塩素化合物を分解する能力とを有する細菌を過酸化水素の存在下で揮発性有機塩素化合物に作用させて、当該揮発性有機塩素化合物を分解する分解工程を含み、前記細菌がロドコッカス(Rhodococcus)属の細菌であるロドコッカス・ジョスティ(Rhodococcus jostii)RHA1株であり、
前記揮発性有機塩素化合物が地下水に含まれており、当該地下水を原位置で浄化し、
前記過酸化水素を、前記地下水中の濃度が0.02%以下になるように導入し、前記ロドコッカス・ジョスティ(Rhodococcus jostii)RHA1株を、前記地下水中の菌数が5.0×108 cells/ml以上になるように導入する、発性有機塩素化合物の浄化方法。
を求める。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2016232127A JP6913454B2 (ja) | 2016-11-30 | 2016-11-30 | 揮発性有機塩素化合物の浄化方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2016232127A JP6913454B2 (ja) | 2016-11-30 | 2016-11-30 | 揮発性有機塩素化合物の浄化方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2018086636A JP2018086636A (ja) | 2018-06-07 |
JP6913454B2 true JP6913454B2 (ja) | 2021-08-04 |
Family
ID=62493180
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2016232127A Active JP6913454B2 (ja) | 2016-11-30 | 2016-11-30 | 揮発性有機塩素化合物の浄化方法 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP6913454B2 (ja) |
-
2016
- 2016-11-30 JP JP2016232127A patent/JP6913454B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
JP2018086636A (ja) | 2018-06-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Ghosal et al. | Current state of knowledge in microbial degradation of polycyclic aromatic hydrocarbons (PAHs): a review | |
Field et al. | Microbial transformation and degradation of polychlorinated biphenyls | |
JPH04502277A (ja) | ハロゲン置換脂肪族炭化水素の生物分解を促進する方法 | |
Chen et al. | Bioremediation of polychlorinated-p-dioxins/dibenzofurans contaminated soil using simulated compost-amended landfill reactors under hypoxic conditions | |
Praveckova et al. | Indirect evidence link PCB dehalogenation with Geobacteraceae in anaerobic sediment-free microcosms | |
Singh et al. | Catabolic enzyme activity and kinetics of pyrene degradation by novel bacterial strains isolated from contaminated soil | |
Gaur et al. | Sustainable strategies for combating hydrocarbon pollution: Special emphasis on mobil oil bioremediation | |
Xu et al. | Long-term dechlorination of polychlorinated biphenyls (PCBs) in Taihu Lake sediment microcosms: Identification of new pathways, PCB-driven shifts of microbial communities, and insights into dechlorination potential | |
Deng et al. | Biodegradation of BTEX aromatics by a haloduric microbial consortium enriched from a sediment of Bohai Sea, China | |
Tu et al. | Enhanced anaerobic biodegradation of OCDD-contaminated soils by Pseudomonas mendocina NSYSU: Microcosm, pilot-scale, and gene studies | |
Matturro et al. | First evidence on the occurrence and dynamics of Dehalococcoides mccartyi PCB-dechlorinase genes in marine sediment during Aroclor1254 reductive dechlorination | |
JP6913454B2 (ja) | 揮発性有機塩素化合物の浄化方法 | |
May et al. | “Dehalobium chlorocoercia” DF-1—from Discovery to Application | |
Deng et al. | Emerging investigator series: environment-specific auxiliary substrates tailored for effective cometabolic bioremediation of 1, 4-dioxane | |
Li et al. | Effective removal of trace 1, 4-dioxane by biological treatments augmented with propanotrophic single culture versus synthetic consortium | |
McCarty | Discovery of organohalide-respiring processes and the bacteria involved | |
Skinner et al. | Implementation of in situ aerobic cometabolism for groundwater treatment: State of the knowledge and important factors for field operation | |
Wu et al. | Development and microbial characterization of Bio-RD-PAOP for effective remediation of polychlorinated biphenyls | |
JP6955886B2 (ja) | Rha1株塩素化合物分解能発現方法、装置 | |
Bealessio | Cometabolism of 1, 4-dioxane and chlorinated aliphatic hydrocarbons by rhodococcus rhodochrous ATCC 21198 and coencapsulated with a slow-release substrate in microcosm and column studies | |
US7462480B2 (en) | Stimulation of microbial dechlorination of polychorinated biphenyls with halogenated ethenes | |
Liu et al. | Complete biodegradation of tetrabromobisphenol A through sequential anaerobic reductive dehalogenation and aerobic oxidation | |
EP3574085B1 (en) | Consortium of microorganisms and method for treating bodies of water contaminated with 1,2-dichloroethane | |
Temme | Connecting Dominant Bacterial Dechlorination Pathways to Environmental Drivers | |
Salas Meza | Effect of Propane Grade on Growth and Activity of Gaseous Hydrocarbon-Oxidizing Bacteria. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A80 | Written request to apply exceptions to lack of novelty of invention |
Free format text: JAPANESE INTERMEDIATE CODE: A80 Effective date: 20161228 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20190827 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20200629 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20200818 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20201019 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20210202 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210421 |
|
C60 | Trial request (containing other claim documents, opposition documents) |
Free format text: JAPANESE INTERMEDIATE CODE: C60 Effective date: 20210421 |
|
A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20210428 |
|
RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7423 Effective date: 20210510 |
|
C21 | Notice of transfer of a case for reconsideration by examiners before appeal proceedings |
Free format text: JAPANESE INTERMEDIATE CODE: C21 Effective date: 20210511 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210629 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210712 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6913454 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |