JP6863074B2 - Micro flow path chip - Google Patents

Description

The present invention relates to a microchannel chip.

In the fields of biomedical and biochemistry, microchannel chips are used mainly for research and development. The microchannel chip is a device in which a microchannel for transporting a fluid such as a reagent is formed, but in many cases, the microchannel chip itself does not have a function of transporting the fluid. Therefore, for example, a pump for transporting a fluid must be separately prepared (see Patent Document 1 and the like). Further, a method of transporting a fluid by centrifugal force by rotating a microchannel chip has also been proposed (see Patent Documents 2 and 3 and the like).

Japanese Unexamined Patent Publication No. 2015-014512 Japanese Unexamined Patent Publication No. 2009-300433 Japanese Unexamined Patent Publication No. 2008-268198

However, in the method of preparing the pump separately, a tube must be used for connecting the pump and the microchannel chip, which increases the dead volume and further complicates the operation. Further, the method of transporting by centrifugal force has a problem that the design of the microchannel becomes complicated and the degree of freedom in design is impaired. In addition, there is a demand for microchannel chips with various characteristics.

The present invention is to provide a microchannel chip that can easily convey a fluid and has various properties.

The microchannel chip according to the first aspect includes a main body portion and a first plunger. The main body portion communicates with the first fluid space for accommodating the first fluid, the first microchannel communicating with the first fluid space, and the first microchannel, and from the first fluid space. It has a reaction space for reacting the first fluid introduced through the first microchannel. The first plunger can move in the first fluid space so as to send the first fluid from the first fluid space to the first microchannel. The main body includes a first member and a second member made of a material different from that of the first member and coupled to the first member.

The microchannel chip according to the second aspect is the microchannel chip according to the first aspect, and the second member is made of a material having higher light transmittance than the first member. The second member at least partially constitutes a side wall defining the reaction space.

The microchannel chip according to the third aspect is the microchannel chip according to the first aspect or the second aspect, and the first member and the second member are attached via an adhesive sheet layer. ..

The microchannel chip according to the fourth aspect is a microchannel chip according to any one of the first to third aspects, and the first fluid space has a plurality of spaces separated from each other, and the said The first plunger has a plurality of plungers each arranged in the plurality of spaces.

The microchannel chip according to the fifth aspect is the microchannel chip according to any one of the first to fourth aspects, and is an introduction port for introducing a sample to react with the first fluid into the reaction space. Further has.

The microchannel chip according to the sixth aspect is the microchannel chip according to the fifth aspect, and the main body portion communicates with the introduction port and is introduced into the reaction space through the introduction port. It also has a sample space for accommodating the sample.

The microchannel chip according to the seventh aspect is the microchannel chip according to the sixth aspect, and further includes a cover that is detachably fixed to the main body so as to cover the sample space.

The microchannel chip according to the eighth aspect is the microchannel chip according to the seventh aspect, and a magnet is provided on one of the surface of the cover facing the main body and the surface of the main body facing the cover. An element that is magnetically coupled to the magnet is arranged on the other side.

The microchannel chip according to the ninth aspect is the microchannel chip according to the seventh aspect, and the cover is fixed to the main body by a luer fitting type or a screw type.

The microchannel chip according to the tenth aspect is the microchannel chip according to the sixth aspect, and further includes a septum fixed to the main body so as to cover the sample space.

The microchannel chip according to the eleventh viewpoint is a microchannel chip according to the sixth to tenth viewpoints, and further includes a second plunger. The main body further includes a second fluid space for accommodating the second fluid, and a second microchannel communicating with the second fluid space and the sample space. The second plunger pushes the sample out of the sample space toward the reaction space by sending the second fluid from the second fluid space to the sample space via the second microchannel. , It is possible to move in the second space.

The microchannel chip according to the twelfth viewpoint is a microchannel chip according to any one of the first to eleventh viewpoints, and the main body is a third microchannel communicating with the reaction space and the above. It communicates with the third microchannel and further has a recovery space for recovering the first fluid from the reaction space via the third microchannel.

The microchannel tip according to the thirteenth aspect is a microchannel chip according to any one of the first to twelfth aspects, and further includes a stopper. The plug can form a block state that blocks the flow of the first fluid from the first fluid space to the first microchannel, and can release the block state.

According to the first aspect, a first fluid space for accommodating a first fluid such as a test solution and a first plunger for delivering the first fluid from the first fluid space are provided. That is, since a syringe including the first fluid space and the first plunger is provided, it is possible to deliver the first fluid by operating the syringe. Therefore, the fluid can be conveyed with a simple configuration.

Further, according to the first aspect, the main body portion includes a first member and a second member made of a material different from that of the first member and coupled to the first member. Thereby, for example, various characteristics can be imparted to the microchannel chip, such as improving the moldability of the microchannel chip and improving the visibility when observing the reaction space formed in the microchannel chip. ..

The figure which shows the structure of the micro flow path chip which concerns on one Embodiment of this invention, and the peripheral device connected to this. FIG. 1 is a sectional view taken along line II-II of FIG. FIG. 1 is a cross-sectional view taken along the line III-III. The cross-sectional view which concerns on the modification. Cross-sectional view according to another modification. Cross-sectional view according to still another modified example.

Hereinafter, the microchannel chip according to the embodiment of the present invention will be described with reference to the drawings.

<1. Microchannel chip configuration>
FIG. 1 shows the configuration of the microchannel chip 1 according to the present embodiment and the peripheral device connected to the microchannel chip 1. In the figure, a plan view of the microchannel chip 1 is shown, and the positional relationship of each part of the microchannels L1 to L6 and the like formed inside the microchannel chip 1 is also shown. 2 and 3 are a sectional view taken along line II-II and a sectional view taken along line III-III of FIG. 1, respectively.

As shown in FIGS. 1 to 3, the microchannel chip 1 has a main body portion 10 which is formed in a block shape having a substantially cubic shape. Inside the main body 10, micro flow paths L1 to L6, which are fine pipelines, are formed, and various spaces S1 to S6 communicating with the micro flow paths L1 to L6 are formed. More specifically, a plurality of (three in the present embodiment) fluid spaces S1 to S3, a sample space S4, a reaction space S5, and a recovery space S6 are formed, and all the spaces S1 to S6 are microflows. It is an open space that is larger in size than the roads L1 to L6. The size referred to here is an area in a plane perpendicular to the direction of movement of the fluid (a plane in the vertical direction of FIGS. 2 and 3 and parallel to the vertical direction), which will be described later. Although the size is not particularly limited, the size of the spaces S1 to S6 can be 3 times or more, more preferably 10 times or more the size of the microchannels L1 to L6. Further, it can be 50 times or more and 100 times or more.

The microchannel chip 1 is not limited to this, but is mainly used for research and development in the fields of biomedical and biochemical fields. The microchannel chip 1 can also be used in POCT (point-of-care testing). In this case, typically, the above fluid spaces S1 and S2 contain reagents, and the sample space S4 contains samples such as blood and urine to be tested by the reagents. The reagent and the sample are usually liquids, but of course, they may be gases. The reaction space S5 is a space in which the reagent and the sample are mixed and reacted. The recovery space S6 is a space for collecting reagents and samples after the reaction and storing them at least temporarily.

The fluid space S1 communicates with the microchannel L1, and the microchannel L1 communicates with the reaction space S5. That is, the fluid space S1 communicates with the reaction space S5 via the microchannel L1. The fluid space S2 communicates with the microchannel L2, and the microchannel L2 communicates with the reaction space S5. That is, the fluid space S2 communicates with the reaction space S5 via the microchannel L2. The reaction space S5 communicates with the microchannel L5, and the microchannel L5 communicates with the recovery space S6. That is, the reaction space S5 communicates with the recovery space S6 via the microchannel L5. The recovery space S6 communicates with the micro flow path L6, and the micro flow path L6 extends to the side surface 10b of the main body 10 and communicates with the external space.

The fluid space S3 communicates with the microchannel L3, and the microchannel L3 communicates with the sample space S4. That is, the fluid space S3 communicates with the sample space S4 via the microchannel L3. The sample space S4 communicates with the microchannel L4, and the microchannel L4 communicates with the reaction space S5. That is, the sample space S4 communicates with the reaction space S5 via the microchannel L4. The fluid space S3 typically contains a fluid for pushing the sample from the sample space S4 through the microchannel L4 into the reaction space S5, preferably a reagent and an inert fluid that does not react with the sample. For example, air is housed in the fluid space S3. The term "inactive" here does not necessarily mean that it does not completely react with the reagent and the sample, but means that it does not react with the reagent and the sample to the extent that it does not interfere with the test of the sample.

The fluid spaces S1 to S3 are cylindrical spaces in the present embodiment, and one end side reaches the side surface 10a of the main body portion 10 along the central axis direction. In other words, the fluid spaces S1 to S3 communicate with the external space through openings 45 to 47 formed on the side surfaces 10a of the main body 10, respectively. On the other hand, the micro flow paths L1 to L3 communicate with the fluid spaces S1 to S3 at the end portion of the main body portion 10 opposite to the side surface 10a.

Plungers 21 to 23 are inserted into the fluid spaces S1 to S3, respectively. The plungers 21 to 23 are configured to be reciprocally movable in the fluid spaces S1 to S3 along the central axis direction of the fluid spaces S1 to S3, respectively. When the plungers 21 to 23 are pushed inward, the fluids in the fluid spaces S1 to S3 are sent out to the microchannels L1 to L3, respectively. That is, in the microchannel chip 1, a plurality of (three in this embodiment) "syringes" are formed by the fluid spaces S1 to S3 and the plungers 21 to 23. These syringes realize the function of transporting the fluid in the fluid spaces S1 to S3.

The plungers 21 to 23 have shaft bodies 21a to 23a, respectively, and have gaskets 21b to 23b at the inner tip portions of the shaft bodies 21a to 23a. The gaskets 21b to 23b can slide smoothly with respect to the side walls of the fluid spaces S1 to S3, respectively, and can maintain the airtightness of the fluid spaces S1 to S3. Therefore, the gaskets 21b to 23b can typically be made of a rubber material, more preferably butyl rubber with less extraction components. Further, in order to improve the slidability of the plungers 21 to 23, it is preferable to apply a lubricant such as silicone grease to at least one of the side walls of the fluid spaces S1 to S3 and the side surfaces of the gaskets 21b to 23b.

The plungers 21 to 23 can be moved manually, but in the present embodiment, they are connected to the drive device 2 controlled by the computer 3. The computer 3 can independently control the operations of the plungers 21 to 23 via the drive device 2. More specifically, the computer 3 can freely control the movement amount of the plungers 21 to 23 and, by extension, the flow velocity of various fluids sent from the fluid spaces S1 to S3. The computer 3 is realized as a general-purpose personal computer including, for example, a control unit such as a CPU, a storage device, an input device, and a display device, and an operator can move the plungers 21 to 23 via the input device, that is, a micro flow rate. The flow velocities of various fluids flowing in the path chip 1 can be set. A dedicated program for causing the control unit to execute the above operations is installed in the storage device.

The specific configuration of the drive device 2 is not particularly limited as long as the plungers 21 to 23 can be reciprocated in the fluid spaces S1 to S3. Since various methods for realizing such mechanical operation are known, detailed description thereof will be omitted here, but to give an example, it can be realized by using a stepping motor. In this case, for example, the shaft bodies 21a to 23a of the plungers 21 to 23 may be connected to the shaft of the stepping motor via an appropriate mechanism capable of converting the rotary motion into a linear motion.

As described above, in the microchannel chip 1, fluid spaces S1 to S3 for accommodating the fluid and plungers 21 to 23 for delivering the fluid from the fluid spaces S1 to S3 are provided. That is, since a syringe composed of the fluid spaces S1 to S3 and the plungers 21 to 23 is provided, it is possible to deliver the fluid in the fluid spaces S1 to S3 by operating the syringe. Therefore, the fluid can be easily conveyed with a simple configuration.

In this embodiment, the sample space S4 is defined by a "dish" 12 formed on the upper surface 10c of the main body 10. An opening 48 communicating with the microchannel L3 is formed on the side surface of the dish 12 that defines the sample space S4. Further, on the bottom surface of the dish 12 that defines the sample space S4, an introduction port 30 is formed that communicates with the microchannel L4 and introduces the sample in the sample space S4 into the reaction space S5 via the microchannel L4. Has been done. From the above, when the plunger 23 is pushed in the fluid space S3, the fluid in the fluid space S3 is pushed out into the sample space S4 via the microchannel L3. At this time, the sample in the sample space S4 is pushed out to the microchannel L4 through the introduction port 30 by the fluid thus flowing into the sample space S4, and finally reaches the reaction space S5 via the microchannel L4. Be transported.

A removable cover 70 that covers the sample space S4 can be arranged on the main body 10. As a result, by opening the cover 70, the sample can be stored in the sample space S4, and after the storage, the sample in the sample space S4 is suppressed from coming into contact with the outside air, and dust or the like is generated in the sample space S4. It is possible to prevent it from entering.

In this embodiment, as shown in FIG. 1, the microchannels L1, L2, and L4 reach the reaction space S5 after merging with each other. Therefore, in the present embodiment, the reagents and samples delivered from the fluid spaces S1 and S2 and the sample space S4 may be mixed to some extent before reaching the reaction space S5. However, these microchannels L1, L2, and L4 may not be merged in the path before reaching the reaction space S5, but may be configured to merge for the first time in the reaction space S5.

Openings 41 to 43 reaching the upper surface 10c of the main body 10 are formed on the paths of the micro flow paths L1 to L3, respectively, and the fluid flows in the micro flow paths L1 to L3 are formed in the openings 41 to 43, respectively. The stoppers 41a to 43a for blocking the above are inserted. As described above, the microchannels L1 to L3 are smaller in size than the spaces S1 to S3 in which the fluid is housed. Therefore, if the microchannels L1 to L3 are not configured to block the flow of the fluid, they gradually become due to the capillary phenomenon. The fluid can move to. The stoppers 41a to 43a are provided to prevent this, and can be appropriately removed during the test of the sample using the microchannel chip 1 to release the blocked state in which the fluid flow is blocked. Of course, after removing the plugs 41a to 43a, the plugs 41a to 43a can be fitted into the openings 41 to 43 again to form a block state again and stop the flow of the fluid as appropriate.

Similarly, an opening 44 reaching the side surface 10b of the main body 10 is formed on the path of the microchannel L6, and the opening 44 is a plug 44a for blocking the flow of fluid in the microchannel L6. Is inserted. The stopper 44a is also removable.

The materials of the stoppers 41a to 44a are not particularly limited, and can be selected from any material such as metal, resin, rubber, and glass. From the viewpoint of mass productivity, it is preferable to select metal or resin. Further, it is preferable to select one having high corrosion resistance, and if it is a metal, SUS304 or the like is preferable. As for the resin, PP (polypropylene), PE (polyethylene), PET (polyethylene terephthalate), PMMA (polymethylmethacrylate), PC (polycarbonate) and the like are preferable.

Further, the recovery space S6 is formed with an opening 49 that reaches the upper surface 10c of the main body 10. The opening 49 is an air hole for adjusting the pressure in the micro flow paths L1 to L6 and the spaces S1 to S6 when the plungers 21 to 23 are pushed. A stopper can also be inserted into the opening 49 before the start of the test.

In the present embodiment, as shown in FIGS. 2 and 3, the main body 10 connects two upper and lower parts, more specifically, a lower first member 51 and an upper second member 52. Manufactured by The reaction space S5 and the recovery space S6 are formed in the first member 51 and are opened on the upper surface of the first member 51, and the opening is closed by the second member 52 (however, the opening is formed in the second member 52). Except for the opening 49 that is made). Further, the sample space S4 is formed in the first member 51 and the second member, and is opened on the upper surface of the second member 52, and the opening is closed by the cover 70 described above. The fluid spaces S1 to S3 are formed in the first member 51 and do not reach the upper surface of the first member 51. The micro flow paths L1 to L5 are formed along the upper surface of the first member 51 while opening mainly, and extend downward in the vicinity of the connecting portion with the fluid spaces S1 to S3. The micro flow path L6 is formed on the side surface 10b of the first member 51 and does not reach the upper surface of the first member 51.

In the present embodiment, by forming the microchannel chip 1 using the first member 51 and the second member 52 having the above-described configuration, the main body portion 10 in which a complicated hollow pattern is formed is formed. It can be manufactured relatively easily.

The material of the first member 51 and the second member 52 is not particularly limited, but is preferably selected from resin, glass, PDMS (dimethylpolysiloxane), rubber and the like. Further, since the reaction in the reaction space S5 can be an object of observation, it is preferable that the first member 51 and the second member 52 are made of a highly transparent material in order to facilitate optical detection of such a reaction. .. From this point of view, for example, resin materials such as PMMA (polymethylmethacrylate), PC (polycarbonate), COC (cycloolefin copolymer), and COP (cycloolefin polymer) are preferably selected. Of these, COP is particularly preferable because it has excellent light transmission. However, in general, a resin material having high light transmission is costly. By the way, from the viewpoint of optically detecting the reaction in the reaction space S5, it is sufficient that at least the observed portion of the portion constituting the side wall defining the reaction space S5 is highly transparent. Therefore, in the present embodiment, the first member 51 and the second member 52 are made of different materials. More specifically, assuming observation from the upper side, the upper second member 52 is made of a material having higher light transmission than the first member 51. For example, the second member 52 can be made of COP, and the first member 51 can be made of PMMA. Of course, when the first member 51 and the second member 52 are made of different materials, not only different types of resins can be bonded to each other, but also resins and rubber, resin and glass, rubber and glass, and the like can be combined. ..

When the first member 51 and the second member 52 are manufactured from a resin material, these members 51 and 52 can be easily manufactured by, for example, injection molding. In this case, the openings 49, the openings 41 to 44, and a part of the microchannels L1 to L6, which serve as air holes, may be additionally machined by cutting instead of being formed at the same time during injection molding.

The method of connecting the first member 51 and the second member 52 is also not particularly limited, but in the present embodiment, both the members 51 and 52 are attached via the pressure-sensitive adhesive sheet layer 60 formed by the adhesive. This method is excellent in that when the first member 51 and the second member 52 are made of different materials, the adhesiveness of both members 51 and 52 can be easily ensured. As the adhesive, a transparent adhesive having a small amount of extraction components is preferable, and for example, an acrylic pressure-sensitive adhesive transfer tape 9769 manufactured by 3M Ltd. can be selected. When the first member 51 and the second member 52 are made of the same material, the bonding surfaces of both members 51 and 52 are heated to the melting point and then the both members 51 and 52 are heat-sealed. The method can also be preferably selected.

<2. How to use the micro flow path chip>
Hereinafter, an example of how to use the microchannel chip 1 will be described, but the method of using the microchannel chip 1 is not limited to this.

First, the microchannel chip 1 is prepared, the stoppers 41a and 42a are removed, and the reagent is injected into the fluid spaces S1 and S2 through the openings 41 and 42 while pulling the plungers 21 and 22. At this time, the gaskets 21b and 22b are left in the fluid spaces S1 and S2. After that, the microchannels L1 and L2 are blocked again by the stoppers 41a and 42a. Alternatively, the plungers 21 and 22 can be removed from the fluid spaces S1 and S2, and the reagent can be injected into the fluid spaces S1 and S2 through the openings 45 and 46. It is also possible to prepare a microchannel chip 1 in which a reagent is stored in advance.

Similarly, the plug 43a is removed, the plunger 23 is pulled, and a sufficient amount of air is filled in the fluid space S3 through the opening 43. At this time, the gasket 23b is left in the fluid space S3. After filling with air, the plug 43a is inserted into the opening 43. However, the stopper 43a is inserted to the extent that the microchannel L3 does not communicate with the external space through the opening 43, but is not inserted to the extent that the microchannel L3 is blocked.

Next, the shaft bodies 21a to 23a of the plungers 21 to 23 are connected to the drive device 2. Further, the cover 70 is opened and the sample is housed in the sample space S4. The sample is, for example, a biological component such as blood or urine. Further, after accommodating the sample, the cover 70 is closed to block the sample space S4 from the external space.

Subsequently, the stoppers 41a and 42a are loosened. More precisely, the microchannels L1 and L2 are not blocked, but the plugs 41a and 42a are inserted so that the microchannels L1 and L2 do not communicate with the external space through the openings 41 and 42. If a plug is attached to the opening 49, it is removed and the recovery space S6 is communicated with the external space through the air hole.

When the above preparations are completed, the computer 3 is operated to drive the drive device 2. As a result, the plungers 21 to 23 are advanced by an appropriate distance at an appropriate speed. The advance speed and advance distance of the plungers 21 to 23 at this time are controlled independently of each other, and as a result, an appropriate amount of test solution and sample are conveyed to the reaction space S5. The plungers 21 to 23 may be driven at the same time or may be driven in order. The test solution reaches the reaction space S5 from the fluid spaces S1 and S2 through the microchannels L1 and L2. On the other hand, the sample is pushed by the air extruded from the fluid space S3 into the sample space S4 and reaches the reaction space S5 through the microchannel L4.

In the reaction space S5, these fluids and samples are mixed to initiate a reaction (including a chemical reaction and a biochemical reaction). Then, this state is observed from the outside using an experimental observation instrument such as an optical microscope or with the naked eye to detect changes in the sample. After the reaction is completed and the observation is completed, the computer 3 is operated to drive the driving device 2 and the plunger 23 is advanced. As a result, air is sent into the reaction space S5, and the fluid after the reaction is pushed out into the recovery space S6 by the air.

After that, if necessary, a new sample can be accommodated in the sample space S4, and the same test can be repeated again. If the fluid space S3 contains a cleaning liquid instead of air in advance, or if the cleaning liquid is introduced after the test, the microchannel L4, the sample space S4, and the reaction space S5 are washed after one test is completed. And the next test can be done in a clean state. Similarly, when the cleaning liquid is stored in one of the fluid spaces S1 and S2 in advance, or when the cleaning liquid is introduced after the test, the next test can be performed in a cleaner state.

After the test is completed, the microchannel chip 1 can be discarded as it is, but the microchannel chip 1 can also be discarded after removing the fluid accumulated in the recovery space S6. In the latter case, by removing the stopper 44a and further advancing the plunger 23, the fluid stored in the recovery space S6 may be pushed out to the external space via the microchannel L6. At this time, it is preferable to close the opening 49 with a stopper or the like.

<3. Modification example>
Although one embodiment of the present invention has been described above, the present invention is not limited to the above embodiment, and various modifications can be made without departing from the spirit of the present invention. For example, the following changes can be made. In addition, the gist of the following modified examples can be combined as appropriate.

<3-1>
The sample space S4 can be omitted. In this case, for example, in the reaction space S5, instead of reacting the sample and the reagent, the reagent and the reagent can be mixed and reacted. Further, the sample space S4 can be omitted, and the sample can be directly accommodated in the reaction space S5. In this case, for example, a cover that can be opened and closed is provided in the reaction space S5, the sample is accommodated in the reaction space S5 via an introduction port formed by opening the cover, and then the cover is closed to start the reaction of the sample. Can be done. Alternatively, an introduction port 30 communicating with the reaction space S5 may be provided on the side wall of the main body 10 defining the reaction space S5, and the sample may be introduced into the reaction space S5 through the introduction port 30.

<3-2>
The micro flow path L6 can be omitted. In this case, the microchannel chip 1 can be discarded while leaving the fluid after the reaction in the recovery space S6. Further, in addition to the microchannel L6, the recovery space S6 can be omitted, and the microchannel L5 can be communicated with the external space instead. This aspect is suitable when the repeated test is not performed. Further, the micro flow paths L5 and L6 and the recovery space S6 can all be omitted. In this case, the microchannel chip 1 can be discarded while leaving the fluid after the reaction in the reaction space S5.

<3-3>
The number of syringes composed of the fluid space and the plunger is not limited to those described above, and may be one, two, or four or more. Further, the fluid delivered from such a syringe is not limited to the above-mentioned reagent and the inert fluid for pushing out the sample, and may be, for example, a cleaning liquid. The type of fluid contained in the syringe is appropriately selected according to the intended use of the microchannel chip 1.

<3-4>
In the above embodiment, the main body 10 is composed of two members 51 and 52, but it can also be configured by connecting three or more members. Of course, it can also be composed of one member.

<3-5>
The number of reaction spaces S5 is not limited to those described above, and a plurality of reaction spaces S5 may be provided. The same applies to the recovery space S6.

<3-6>
The cover 70 that covers the sample space S4 can be detachably fixed to the main body 10. The sample space S4 is blocked from the external space by being covered with the cover 70. In this case, for example, as shown in FIG. 4, the element 71 is arranged on the surface of the cover 70 facing the main body 10, the element 72 is arranged on the surface of the main body 10 facing the cover 70, and the elements 71 and 72. One can be a magnet and the other can be an element that is magnetically coupled to this magnet. Both elements 71 and 72 can be magnets. At this time, it is preferable that the elements 71 and 72 are arranged so as to be embedded in the cover 70 and the main body 10, respectively.

Further, to give another example, the cover 70 can be detachably fixed to the main body 10 by a screw type or a luer fitting type. In this way, the cover 70 and the main body 10 can be fixed by a screw type. In this case, for example, as shown in FIG. 5, the portion of the main body 10 that forms the upper opening of the sample space S4 may be the cylindrical portion 75, and a screw thread may be formed on the outer peripheral surface of the cylindrical portion 75. Then, a thread threaded on the thread of the cylindrical portion 75 can be formed on the surface of the cover 70 facing the outer peripheral surface of the cylindrical portion 75.

<3-7>
As shown in FIG. 6, a septum as a cover 70 fixed to the main body 10 can be provided so as to cover the sample space S4. The sample space S4 is blocked from the external space by being covered with the septum. In this case, the sample can be injected into the sample space S4 by preparing a syringe containing the sample and piercing the septum with the needle of the syringe.

1 Micro flow path chip 10 Main body 21 and 22 Plunger (1st plunger)
23 Plunger (2nd plunger)
30 Introductory port 41a to 44a Plug 51 First member 52 Second member 60 Adhesive sheet layer 70 Cover S1, S2 Fluid space (first fluid space)
S3 fluid space (second fluid space)
S4 Specimen space S5 Reaction space S6 Recovery space L1, L2 Microchannel (first microchannel)
L3 micro flow path (second micro flow path)
L4 micro flow path L5 micro flow path (third micro flow path)

Claims (9)

A first fluid space for accommodating a first fluid, a first microchannel communicating with the first fluid space, and a first microflow flowing from the first fluid space communicating with the first microchannel. A main body having a reaction space for reacting the first fluid introduced through the path, and
A first plunger that can move in the first fluid space so as to send the first fluid from the first fluid space to the first microchannel.
A cover that is detachably fixed to the main body is provided.
The main body includes a first member and a second member made of a material different from that of the first member and coupled to the first member.
The main body contains an introduction port for introducing a sample to be reacted with the first fluid into the reaction space, and the sample that communicates with the introduction port and is introduced into the reaction space through the introduction port. Has a sample space for
The sample space is formed in a dish shape having side surfaces and a bottom surface while opening on the upper surface of the main body portion.
The inlet is formed on the bottom surface of the sample space.
The cover covers the opening of the sample space, and magnets are arranged on one of the surface of the cover facing the main body and the surface of the main body facing the cover, and the magnet is magnetically applied to the other. The elements to be combined are arranged,
Micro flow path chip. The magnet is embedded in one of the surface of the cover facing the main body and the surface of the main body facing the cover, and an element magnetically coupled to the magnet is embedded in the other.
The microchannel chip according to claim 1. A first fluid space for accommodating a first fluid, a first microchannel communicating with the first fluid space, and a first microflow flowing from the first fluid space communicating with the first microchannel. A main body having a reaction space for reacting the first fluid introduced through the path, and
A first plunger that can move in the first fluid space so as to send the first fluid from the first fluid space to the first microchannel.
A cover that is detachably fixed to the main body is provided.
The main body includes a first member and a second member made of a material different from that of the first member and coupled to the first member.
The main body contains an introduction port for introducing a sample to be reacted with the first fluid into the reaction space, and the sample that communicates with the introduction port and is introduced into the reaction space through the introduction port. Has a sample space for
The sample space is formed in a dish shape having a side surface and a bottom surface while opening on the upper surface of the main body portion.
The inlet is formed on the bottom surface of the sample space.
The cover is fixed to the main body by a luer fitting type or a screw type so as to cover the opening of the sample space.
Micro flow path chip. In the main body portion, the portion forming the opening of the sample space is a cylindrical portion, and a first screw thread is formed on the outer peripheral surface of the cylindrical portion, and the cover faces the outer peripheral surface. A second thread that is screwed into the first thread is formed on the surface to be threaded.
The microchannel chip according to claim 3. A first fluid space for accommodating a first fluid, a first microchannel communicating with the first fluid space, and a first microflow flowing from the first fluid space communicating with the first microchannel. A main body having a reaction space for reacting the first fluid introduced through the path, and
A first plunger that can move in the first fluid space so as to send the first fluid from the first fluid space to the first microchannel.
A cover that is fixed to the main body is provided.
The main body includes a first member and a second member made of a material different from that of the first member and coupled to the first member.
The main body contains an introduction port for introducing a sample to be reacted with the first fluid into the reaction space, and the sample that communicates with the introduction port and is introduced into the reaction space through the introduction port. Has a sample space for
The sample space is formed in a dish shape having a side surface and a bottom surface while opening on the upper surface of the main body portion.
The inlet is formed on the bottom surface of the sample space.
The cover is a septum fixed to the main body so as to cover the opening of the sample space.
Micro flow path chip. The peripheral edge portion defining the opening of the sample space rises upward on the upper surface of the main body portion, and the septum is fixed to the rising portion.
The microchannel chip according to claim 5. The main body further has a second fluid space for accommodating the second fluid and a second microchannel communicating with the second fluid space.
By sending the second fluid from the second fluid space to the sample space via the second microchannel, the sample is pushed out from the sample space toward the reaction space in the second space. 2nd plunger that can be moved
With more
A side opening communicating with the second microchannel is formed on the side surface of the sample space.
The microchannel chip according to any one of claims 1 to 6. The main body further has a second fluid space for accommodating the second fluid and a second microchannel communicating with the second fluid space.
By sending the second fluid from the second fluid space to the sample space via the second microchannel, the sample is pushed out from the sample space toward the reaction space in the second space. 2nd plunger that can be moved
With more
The first plunger and the second plunger are arranged so as not to overlap each other and to be parallel to each other when viewed from the opening side of the sample space.
The microchannel chip according to any one of claims 1 to 7. The main body further has a second fluid space for accommodating the second fluid and a second microchannel communicating with the second fluid space.
By sending the second fluid from the second fluid space to the second microchannel, the second fluid moves in the second space so as to be pushed out from the second fluid space toward the reaction space. Possible second plunger
With more
The first plunger and the second plunger are arranged so as not to overlap each other and to be parallel to each other when viewed from the opening side of the sample space.
The microchannel chip according to any one of claims 1 to 6.

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