JP6778035B2 - Test piece for detecting components in body fluids - Google Patents

Description

The present invention relates to a test piece for detecting components in body fluids, and in particular, detects the presence or absence of ketone bodies in urine or ketone bodies as skin gas components, early estimation of dehydration or dehydration symptoms, confirmation of diet effect, The present invention relates to a test piece for detecting components in body fluid, which can be used for early diagnosis of diabetes.

Medically, there are numerous diseases that present with dehydration. It is often observed that various infectious diseases, such as colds, pneumonia, and influenza, which are mainly accompanied by fever, become dehydrated without being noticed without careful and sufficient hydration.
In addition, when suffering from infectious gastroenteritis, particularly viral gastroenteritis such as norovirus infection and rotavirus infection, the symptoms such as vomiting and diarrhea are strong, so that water is lost and dehydration occurs immediately.

Furthermore, due to the recent warming phenomenon, in the summer, there are many dehydrations due to heat stroke during outdoor activities, and in order to limit the use of air conditioners not only in outdoor activities but also indoors to prevent the invasion of hot air, It is often reported that people suffer from heat stroke and dehydration when they live in a closed state by using only electric fans.
As described above, dehydration is easily caused in a dry environment, a closed space, a disorder of physical adaptation in a hot environment, and a situation such as fever, vomiting, and diarrhea.

In clinical practice, dehydration occurs when body fluids decrease due to various factors, but when this dehydration becomes severe, it causes multiple organ symptoms such as acute circulatory failure, acute renal failure, shock, and spasm. It requires rapid examination, early diagnosis, and appropriate treatment to be concomitant and fatal.

Generally, in the medical field, the presence or absence of urinary ketone bodies is measured as one means of diagnosing whether or not the patient is suffering from dehydration.
Ketone bodies are components (incomplete metabolites) synthesized from fatty acids and amino acids produced in the liver, and are a general term for acetone, acetoacetic acid, and β-hydroxybutyric acid.
This ketone body mainly functions as an energy source for various organs such as the heart and kidneys, which are indispensable for life support. That is, the basic energy source is glucose (dextrose), but ketone bodies are produced as a substitute energy source when glucose is no longer produced due to some kind of disorder or disease.
Therefore, ketone bodies are mainly produced when the living body is not functioning normally, such as glucose deficiency in the body and abnormal glucose metabolism.

That is, in the case where the blood glucose level, which is an index indicating blood glucose, is abnormally low, the sugar content (glucose) as an energy source is insufficient. In this case, the liver decomposes fat to produce fatty acids. , This fatty acid is used as an energy source. However, when the production of this fatty acid exceeds a certain amount, acetone, acetoacetic acid, and β-hydroxybutyric acid are newly produced, and these components are ketone bodies.
When the concentration of such ketone bodies in blood exceeds a certain amount, they are excreted in urine, and the amount of ketone bodies in urine also increases.
Therefore, ketone bodies rise even when a sufficient energy source (glucose) is not supplied to the body due to dehydration, etc. Therefore, when dehydration occurs, the blood sugar level drops with lack of water, and urinary ketone bodies rise. Is observed.
From this point of view, confirmation of the presence or absence of urinary ketone bodies is used as one means of diagnosing dehydration.

So far, urinalysis test paper (Uropaper) (Non-Patent Document 1) using an indicator that discolors upon contact with urine has been used as a test paper for detecting components such as ketone bodies in urine and urine pH. ing.
This uropaper can be tested for urine pH, urine sugar, urine protein, etc. by reacting with the test reagent upon contact with urine and discoloring. Among them, especially for detecting urinary ketone bodies. As an indicator, uropaper by the so-called nitropurside method, which discolors when it comes into contact with urinary ketone bodies, is used.

By the way, in general, it is indispensable to collect urine in order to diagnose dehydration at a medical institution, but in reality, it is extremely difficult to collect urine from children such as babies and toddlers, or elderly patients. is there.
Therefore, for example, as a means for easily inspecting the components in urine, a means for separating and collecting urine has been proposed, but since the operation is complicated and it is necessary to have specialized knowledge, it is generally used. It is difficult to spread the word (Patent Document 1).
On the other hand, as a sign when urinating, there is a means for visually confirming by providing a line or pattern that detects moisture in urine and discolors inside the waterproof sheet of a paper diaper (Patent Document 2), but urination is detected. However, it does not have functions such as early estimation of dehydration, confirmation of diet effect, and early diagnosis of diabetes.

Biological information is acquired by various methods for the treatment of various diseases, and the above-mentioned detection of urinary ketone bodies is one of the means, but as a sample (biological sample) obtained from the living body. , Ketone bodies of sweat (acetoacetic acid) as body fluid from the skin surface, or ketone bodies (acetone) as gas released from the skin surface (skin gas), and its detection is also one of the important biological information. Conceivable.

In particular, skin gas reflects the state of health of the body, and the detection of acetone, which is a ketone body, is a merkmal of advanced states such as diabetes, starvation, malnutrition, and diet, that is, a health monitor or an exercise measurement monitor. Although a "skin gas acetone analyzer" has been proposed (Patent Document 3), development of a test piece for detecting components in body fluid that easily measures acetone released from the skin, which is a skin gas. The current situation is that it has not been done.

Publication of Patent No. 4550192 Japanese Unexamined Patent Publication No. 2004-229695 Publication of Patent No. 5412609

Japanese Committee for Clinical Laboratory Standards (LCCLS) Proposed Guideline "Urine Test Strip Inspection Method" (Supplement-version, 2004)

Therefore, the present invention is a means capable of diagnosing dehydration, clearly detecting the presence or absence of urinary ketone bodies, or detecting the presence or absence of ketone bodies (acetones) as a skin gas component, and causing dehydration or dehydration. An object of the present invention is to provide a test piece for detecting components in body fluid, which can be used for early estimation, confirmation of diet effect, early diagnosis of diabetes, etc., and its usage.

In order to solve this problem, the present inventor has made diligent studies, for example, deterioration of nitropulside sodium, which is a test component of ketone bodies, as a test indicator capable of detecting ketone bodies in urine or ketone bodies (acetones) as skin gas. By making the structure capable of preventing the above, a coloring reaction due to contact with a urinary ketone body or a ketone body as a skin gas (acetone) is clearly generated, and the discoloration due to the coloring reaction is visually observed. Focusing on the fact that it is possible to detect the presence or absence of ketone bodies in urine, the present invention has been completed as a result.

That is, the present invention has, as its basic aspect,
(1) The first member to which the basic reagent was added and
A second member to which a body fluid component test reagent that reacts with a body fluid component and develops color under at least alkaline conditions is added, which comes into contact with the first member.
Consists of
Detection of components in body fluid, characterized in that the body fluid absorbed from the first member side infiltrates and migrates to the second member side under alkaline conditions to cause a color reaction with a body fluid test reagent. It is a test piece for use.

As a more specific embodiment, the present invention
(2) The test piece for detecting a component in a body fluid according to (1) above, wherein the first member and the second member are sheet-like members such as a woven fabric, a non-woven fabric, a porous body, a film or a paper material;
(3) The contact between the first member and the second member is a laminated structure in which both are laminated, the second member is incorporated in a part region of the first member, or the contact is made in a part region of the second member. It is characterized in that the enclosed structure incorporating the first member or the printed structure in which the second member or the first member is engraved in the first member or the second member is brought into contact with the above ( Test piece for detecting components in body fluid according to 1) or (2);
(4) In the body fluid according to (1) above, the basic reagent to be added to the first member is an alkali metal or alkaline earth metal carbonate, or an alkali metal or alkaline earth metal hydrogen carbonate. Specimen for component detection;
(5) The alkali metal or alkaline earth metal carbonate, or the alkali metal or alkaline earth metal hydrogen carbonate is sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium hydrogen carbonate, calcium carbonate, calcium hydrogen carbonate. Test piece for detecting components in body fluid according to (4) above;
(6) The test piece for detecting a component in a body fluid according to (1) above, wherein the reagent for testing the component of the body fluid component to be added to the second member is sodium nitroprusside;
(7) The test piece for detecting a component in a body fluid according to (6) above, which is impregnated with a body fluid component test reagent to be added to the second member under acidic conditions;
(8) The body fluid according to (6) or (7) above, wherein a light-resistant reagent and / or a heat-resistant reagent is further added in addition to the body fluid component test reagent added to the second member. Specimen for component detection;
(9) The test piece for detecting components in body fluid according to (8) above, wherein the light-resistant reagent is sodium carbazochrome sulfonate and flavin adenine dinucleotide sodium, and the heat-resistant reagent is tartaric acid;
Is.

Further, the present invention
(10) The test piece for detecting a component in a body fluid according to any one of (1) to (9) above, wherein the detection of a component in the body fluid is the detection of a ketone body in urine;
(11) The test piece for detecting a component in a body fluid according to any one of (1) to (9) above, wherein the detection of a component in the body fluid is the detection of a ketone body in skin gas;
Is.

Furthermore, the present invention
(12) The test piece for detecting a component in a body fluid according to any one of (1) to (11) above, which is used for early estimation of dehydration, confirmation of diet effect, and early estimation of diabetes;
Is.

The test piece for detecting components in body fluid provided by the present invention clearly causes a coloration reaction due to contact with a urinary ketone body, and by visually observing the discoloration due to the coloration reaction, the presence or absence of a urinary ketone body or the like is observed. By applying this test piece to paper omelets, it is possible to detect the presence or absence of urinary ketone bodies in children such as infants and toddlers who have extremely difficult urine collection, or in the elderly. Its medical effect is specific in that it can be visually detected from the body, dehydration can be quickly diagnosed, and appropriate treatment for dehydration can be given.

Furthermore, by applying the test piece for detecting components in body fluid provided by the present invention to, for example, an adhesive sheet or a wristband, a ketone body (acetone) as a skin gas or a ketone body (acetoacetic acid) in sweat can be applied. (Acetoacetic acid) can be detected, and discoloration due to skin gas, etc. due to the test piece can be visually detected from the outside, and daily early estimation of dehydration, confirmation of diet effect, early diagnosis of diabetes, etc. In terms of being able to do this, its applicability is extremely great, and it contributes to daily health management.

It is a cross-sectional enlarged view based on one Example of the test piece for detecting a component in a body fluid of this invention. It is sectional drawing based on another Example of the test piece for detecting a component in a body fluid of this invention. This is an example of applying the test piece for detecting a component in a body fluid of the present invention, and is a top view of a sticking plaster type sticking sheet with a test piece combined with an adhesive sheet, as viewed from the test piece side. It is sectional drawing of the line AA in FIG. As an example of applying the pressure-sensitive adhesive sheet with a test piece shown in FIG. 3 to a paper diaper, it is a perspective view showing a state in which the paper diaper is spread out. It is the schematic of the wristband which is an example which applied the test piece for detecting the component in a body fluid of this invention.

As described above, the basic aspect of the present invention is
The first member to which the basic reagent was added and
A second member to which a body fluid component test reagent that reacts with a body fluid component and develops color under at least alkaline conditions is added, which comes into contact with the first member.
Consists of
Detection of components in body fluid, characterized in that the body fluid absorbed from the first member side infiltrates and migrates to the second member side under alkaline conditions to cause a color reaction with a body fluid test reagent. Test piece,
Is.

Generally, "Uropaper" is clinically known as a test paper for detecting urinary ketone bodies as a component in body fluids. This uropaper is a test paper impregnated with sodium nitroprusside, which is a ketone body detection reagent. Oxime is generated by contact with a ketone body under basic conditions, and this oxime turns purple. It is used as a presentational reaction.
As such uropaper, for example, uropaper IIIK (manufactured by Eiken Chemical Co., Ltd.) is commercially available.

According to the study by the present inventors, these commercially available uropapers deteriorate when they are taken out of the storage bottle and left in the air for several days, and do not show a color reaction with ketone bodies. Therefore, it is almost impossible to use uropaper itself as a urinary ketone body test indicator as a component in body fluids.
Therefore, the present inventors have attempted to prepare a urinalysis test piece as a test cloth in which sodium nitroprusside, which is a test reagent for uropaper, is prepared as a solution under alkaline conditions and impregnated into, for example, a non-woven fabric. However, even in this case, even if it is left in the air for several days under dry conditions, it deteriorates and does not show a color reaction with a ketone body as a test piece.

In view of such a situation, the present inventors diligently studied the components in the body fluid, specifically, the first member to which a basic reagent was added as a test piece for a urinary ketone body, and the urinary ketone body. It is formed as a composite member with the second member to which sodium nitropulside, which is a detection reagent, is added, and the first member and the second member are brought into contact with each other to keep sodium nitropulside under alkaline conditions until just before contact with a body fluid. It has been found that deterioration of the color-developing reaction as an inspection test piece can be prevented by constructing the test piece without any problem.
Therefore, the present invention is characterized in that, as an inspection test piece for detecting a component in a body fluid, for example, a urinary ketone body, the test piece is composed of a composite member composed of the first member and the second member described above. Is.

The first member constituting the composite member in this case is a sheet-like member which is a woven fabric, a non-woven fabric, a porous body, a film or a paper material, and the second member is a woven fabric, a non-woven fabric, or a porous material. It is preferably a sheet-like member which is a body, a film or a paper material.

Further, the form of the composite member as the detection test piece of the present invention is, for example, a laminated structure composed of a first member and a second member, or an embedded structure in which another member is embedded in one of the members. Alternatively, it can take the form of an enclosed structure in which the other member is enclosed in one of the members, and further, a mixed structure by weaving, knitting, twisting, etc., and among them, the first member. It is preferable to adopt a laminated structure composed of the second member.

Therefore, the "test piece for detecting components in body fluid" referred to in the present invention is interpreted as having the meaning of a "test piece", that is, a device, a component, an element, or the like as a test piece. You should be careful.

Examples of the basic reagent to be impregnated in the first member constituting the composite member include alkali metal or alkaline earth metal bicarbonate that becomes alkaline upon contact with urine as a body fluid or sweat, or alkali metal or alkaline soil. Examples thereof include basic reagents that are similar metal hydrogen carbonates, and specific examples thereof include sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium hydrogen carbonate, calcium carbonate, calcium hydrogen carbonate and the like.

The amount added is sufficient so that the first member can come into contact with a body fluid (for example, urine) and cause a color reaction with sodium nitroprusside, which is a urinary ketone body detection reagent added into the second member. The amount may be a value of about pH 9 to 12 that maintains a weak alkalinity, and it is not preferable to add an amount that raises the pH.

On the other hand, for example, sodium nitroprusside, which is a ketone body test reagent for detecting ketone bodies in urine, is added to the second member, but this second member deteriorates due to direct exposure to external light. Alternatively, it is also preferable to impart light resistance and / or heat resistance / moisture resistance for the purpose of preventing deterioration due to temperature / humidity from the outside.
As a means for imparting such light resistance, for example, a means for adding a combination of sodium carbazochrome sulfonate and flavin adenine dinucleotide sodium can be mentioned.

Since sodium carbazochrome sulfonate is originally a yellow reagent, it may hinder the confirmation of discoloration change of the test reagent. Therefore, it is not always necessary to add it, and when it is added, it is preferable that the amount does not hinder the confirmation of the discoloration change.

Specifically, the addition of heat resistance / moisture resistance is considered in consideration of the response to changes in temperature / humidity depending on the season. As means for imparting such heat resistance / humidity resistance, for example, tartaric acid, citric acid, etc. Examples of the means for carrying out the method are to include an acidic solution having a pH of about 1 to 6 with citric acid or the like.

In the body fluid component test test piece of the present invention constructed as described above, the components contained in the body fluid, for example, a ketone body and the test test drug are presented by contact with the body fluid (for example, urine, sweat, etc.). Skin gas (acetone) released from the surface of the skin causes a color reaction, and the test drug causes a color reaction, recognizing the confirmation of the presence of components in body fluids, early estimation of dehydration or dehydration, diet It is possible to confirm the effect and diagnose diabetes at an early stage.

Hereinafter, the test piece for detecting a component in a body fluid of the present invention will be described in detail with reference to an example based on the drawings, but the present invention is not limited to these examples, and various modifications are performed. It goes without saying that such a modified method is also included in the present invention as long as it does not deviate from the scope of claims.

FIG. 1 is an enlarged cross-sectional view based on an example of a test piece for detecting a component in a body fluid provided by the present invention (hereinafter, may be simply referred to as a “test piece”).
In the figure, the test piece 1 has a laminated structure including a first member 101 and a second member 102.
The first member 101 is a sheet-like member which is a woven fabric, a non-woven fabric, a porous body, a film, or a paper material to which a basic reagent is added, and the second member 102 is a body liquid component detection reagent, for example, a ketone body detection. A sheet-like member such as a woven fabric, a non-woven fabric, a porous body, a film, or a paper material to which sodium nitroprusside, which is a reagent, is added.

In the test piece of the present invention based on the present embodiment, the first member 101 and the second member 102 are laminated as a structure in which the first member 101 and the second member 102 are in contact with each other (contact), and a basic reagent is applied together with the body fluid from the first member 101 side. The pH of the second member 102 is set to the alkaline side by the basic reagent added to the first member 101 after infiltration and permeation, and the color reaction between the added ketone body detection reagent, nitropulside sodium, and the ketone body. Is clearly generated, and the presence of components in the body fluid can be visually confirmed by observing the discoloration of the second member 102.

A more specific example of the above-mentioned test piece 1 provided by the present invention is shown below.
90 g / m ² of Japanese Industrial Standards (JISP3801) No. 1 filter paper and 75 g / m ² of sodium carbonate were combined to form the first member.
On the other hand, 90 g / m ² of Japanese Industrial Standards (JISP3801) No. 1 filter paper and 50 g / m ² of glycine and 3 g / m ² of sodium nitroprusside were combined to form a second member.
The first member and the second member obtained above were each 1 cm square, and both were laminated to prepare a test piece 1.

The results of detection of ketone bodies in other liquids using the test piece 1 obtained above are shown.
That is, in the contact between normal urine and the test piece 1 in the state where there are no ketone bodies in the urine, the reaction with the added reagent does not occur and the color change in the test piece 1 does not occur (negative judgment). However, if a urinary ketone body is present, the test piece 1 turns purple due to the color reaction with the nitropulside sodium impregnated in the second member 102 of the test piece 1 due to contact with the ketone body. It changed and was judged to be positive for ketone bodies.

FIG. 2 shows a test piece based on another embodiment of the present invention.
In this example, in the test piece 1, the body fluid component detection reagent, for example, sodium nitroprusside, which is a ketone body detection reagent, is added to a part of the region in the first member 101 to which the basic reagent is added. The structure is such that the member 102 is incorporated.
Incorporation of the first member 101 and the second member 102 is the opposite of the above, in which the basic reagent is added to a part of the region in the second member 102 to which sodium nitroprusside, which is a ketone body detection reagent, is added. The member 101 may be incorporated, and the state of such a configuration is shown in parentheses.

In this embodiment as well, as in the previous embodiment, the basic reagent is infiltrated and permeated into the second member 102 together with the body fluid from the first member 101 side, and the pH of the second member 102 becomes the alkaline side and is added. The color reaction between sodium nitroplucid, which is a ketone body detection reagent, and the ketone body clearly occurs, and the presence of components in the body fluid can be visually confirmed by observing the discoloration of the second member 102.

The test piece for detecting a component in a body fluid provided by the present invention is not limited to the above-described embodiment as its embodiment, and as another embodiment, for example, in the first member 101 or the second member 102. The second member 102 or the first member 102 may be used to form a printed structure in which markings, for example, characters, figures, etc. are applied.

Some application examples using the test piece of the present invention configured as described above will be described with reference to the drawings.

FIG. 3 shows a top view of a so-called adhesive plaster type sticking sheet with a test piece, which is a combination of an adhesive tape and a test piece (test piece) for detecting a component in a body fluid of the present invention, and has a sticking function, as seen from the test piece side. ..
That is, the sticking sheet 20 has a form in which the test piece 1 of the present invention is laminated on the central portion of the adhesive layer 21 of the rectangular sheet.
In FIG. 3, the sticking sheet 20 has a horizontally long rectangle (rectangle), and various shapes such as a square shape, a rhombus shape, a round shape, and a star shape can be adopted. The size is not particularly limited as long as it has a sticking surface exceeding the test piece 1, and may be a size of about 3 to 6 cm.

FIG. 4 shows a cross-sectional view taken along the line AA in FIG.
That is, the sticking sheet 20 is stretchable or non-stretchable, and the adhesive layer 21 is provided on one surface of the moisture-permeable or non-permeable support 22, and the test piece 1 of the present invention is provided at the center of the adhesive layer 21. Has a form of being laminated.
The test piece 1 is preferably laminated on the sticking side so as to be on the first member side of the test piece shown in FIG. 1, for example, and in that case, the first member (the first member to which the basic reagent is added). It is preferable to cover the side (one member) with, for example, a porous sheet to suppress irritation to the human skin, which is the sticking surface.

As the support 22, the adhesive layer 22, and the porous sheet in the pressure-sensitive adhesive sheet 20, those generally used for external preparations such as a patch and a poultice can be applied as they are.

As an application thereof, the sticking sheet 20 incorporating the test paper 1 of the present invention shown in FIG. 3 is a test piece that releases ketone bodies in body fluid (sweat) or acetone by skin gas by sticking to the surface of the human body. It can be recognized by the discoloration of 1 and, for example, the exercise effect, diet effect, etc. due to exercise in a sports gym or the like can be confirmed.

Further, FIG. 5 shows a perspective view showing a state of being attached to a paper diaper as an example of applying the attachment sheet provided with the test piece of the present invention shown in FIG.
In the figure, 30 indicates a paper diaper, and this paper diaper is a sticking sheet 20 provided with the test piece of the present invention shown in FIG. 3 in the vicinity of the urination site of the absorption portion 31 on the front abdominal side constituting the paper diaper. It is constructed by pasting.
By attaching the test paper of the present invention to this paper diaper, the presence or absence of ketone bodies in urine can be detected when the diaper is replaced, and dehydration can be estimated at an early stage.

Furthermore, as another application example of the sticking sheet 20 shown in FIG. 3, the presence or absence of urinary ketone bodies can be detected by sticking to a urine leak pad.
In these cases, in addition to attaching the attachment sheet 20 shown in FIG. 3, a paper diaper or a urine leakage pad may be formed by appropriately attaching the test piece itself of the present invention. Needless to say, it's good.

Further, as an application example for detecting the presence or absence of ketone bodies in body fluids during urination using the test piece itself provided by the present invention, for example, a test paper is incorporated into the tip of a stick-shaped elongated paper to urinate. By directly contacting with urine, it is possible to observe the color change of the test paper and make an early estimation of dehydration.
Furthermore, especially in the case of women, by incorporating it into urine wiping napkins (tissue paper) in the groin area after urination and bringing it into direct contact with urine during urination, the color change of the test paper is observed and dehydration occurs. Early inference of symptoms can be made.

Further, as an application example of the test piece provided by the present invention, an example of incorporating it into a wristband for detecting skin gas (acetone) is shown in FIG.
FIG. 6 is a perspective view of a wristband 400 in which the test piece 1 of the present invention is provided as a part of the arm-shaped band 41.

The wristband 40 can be worn on the arm, and the release of acetone, which is a skin gas, can be recognized by the discoloration of the test piece 1 by exercising in a sports gym or the like, and the diet effect can be confirmed.
Furthermore, it is also possible to detect ketone bodies (acetoacetic acid) contained in the sweat of the skin.
Skin gas reflects the health of the body, and acetone as skin gas is released as a merkmal with diabetes, starvation, and malnutrition, and by recognizing the discoloration of the test piece in the wristband, dehydration. It is possible to make an early estimation of the disease, confirm the diet effect, and make an early diagnosis of diabetes.

As described above, according to the present invention, it is possible to provide a test piece for detecting urinary ketone bodies that can detect the presence or absence of urinary ketone bodies from the outside, and by applying such a test piece, urine Its medical effect is specific in that the presence or absence of dehydration can be quickly determined by detecting middle ketone bodies, and appropriate treatment for dehydration can be performed.
Further, the test piece for detecting a component in a body fluid provided by the present invention can confirm a diet effect, an early diagnosis of diabetes, and the like, and has great industrial applicability.

1 Test piece for detecting components in body fluid 101 1st member 102 2nd member 20 Adhesive sheet with test piece 21 Adhesive layer 22 Support 30 Paper diaper 31 Absorbent 40 Wristband 41 Arm-shaped band

Claims (11)

The first member to which the basic reagent was added and
A second member to which a body fluid component test reagent that reacts with a body fluid component and develops color under at least alkaline conditions is added, which comes into contact with the first member.
Consists of
For the contact between the first member and the second member, the second member is incorporated in a part region of the first member, the first member is incorporated in a part region of the second member, or the first member is incorporated. Abutment is made in one member or in the second member so as to have a printed structure in which the second member or the first member is engraved.
Detection of components in body fluid, characterized in that the body fluid absorbed from the first member side infiltrates and migrates to the second member side under alkaline conditions to cause a color reaction with a body fluid test reagent. Test piece for. The test piece for detecting a component in a body fluid according to claim 1, wherein the first member and the second member are a sheet-like member made of a woven fabric, a non-woven fabric, a porous body, a film or a paper material. The test for detecting components in body fluid according to claim 1, wherein the basic reagent added to the first member is an alkali metal or alkaline earth metal carbonate, or an alkali metal or alkaline earth metal hydrogen carbonate. Piece. Claim 3 that the alkali metal or alkaline earth metal carbonate, or the alkali metal or alkaline earth metal hydrogen carbonate is sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium hydrogen carbonate, calcium carbonate, calcium hydrogen carbonate. The test piece for detecting components in body fluid described in 1. The test piece for detecting a component in a body fluid according to claim 1, wherein the reagent for testing the component of the body fluid component to be added to the second member is sodium nitroprusside. The test piece for detecting a component in a body fluid according to claim 5 , wherein the body fluid component test reagent to be added to the second member is impregnated under acidic conditions. The test piece for detecting a component in a body fluid according to claim 5 or 6 , wherein a light-resistant reagent and / or a heat-resistant reagent is further added in addition to the body fluid component test reagent added to the second member. The test piece for detecting a component in a body fluid according to claim 7 , wherein the light-resistant reagent is sodium carbazochrome sulfonate and flavin adenine dinucleotide sodium, and the heat-resistant reagent is tartaric acid. The test piece for detecting a component in a body fluid according to any one of claims 1 to 8 , wherein the detection of a component in the body fluid is the detection of a ketone body in urine. The test piece for detecting a component in a body fluid according to any one of claims 1 to 8 , wherein the detection of the component in the body fluid is the detection as a ketone body in the skin gas contained in the body fluid. The test piece for detecting a component in a body fluid according to any one of claims 1 to 10 , which is used for early estimation of dehydration, confirmation of diet effect, and early estimation of diabetes.

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