JP6749916B2 - Method for producing fermented milk containing high concentration of protein - Google Patents

Method for producing fermented milk containing high concentration of protein Download PDF

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JP6749916B2
JP6749916B2 JP2017535237A JP2017535237A JP6749916B2 JP 6749916 B2 JP6749916 B2 JP 6749916B2 JP 2017535237 A JP2017535237 A JP 2017535237A JP 2017535237 A JP2017535237 A JP 2017535237A JP 6749916 B2 JP6749916 B2 JP 6749916B2
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JPWO2017029803A1 (en
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誠二 長岡
誠二 長岡
成子 金子
成子 金子
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Meiji Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
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Description

本発明は、タンパク質を高濃度で含む発酵乳の製造方法に関する。 The present invention relates to a method for producing fermented milk containing a high concentration of protein.

タンパク質を高濃度で含む発酵乳(高タンパク質含有の発酵乳)として、クワルク、フレッシュチーズ、ギリシャヨーグルト等の名称を付したものが知られている。これらの高タンパク質含有の発酵乳は、通常の製造方法で製造した発酵乳に膜分離処理や遠心分離処理を行い、ホエイを除去する濃縮工程を経て製造されることが一般的である。 As fermented milk containing protein in a high concentration (fermented milk containing high protein), those given names such as quark, fresh cheese and Greek yogurt are known. Fermented milk containing these high proteins is generally produced by subjecting the fermented milk produced by an ordinary production method to a membrane separation process or a centrifugal separation process, and a concentration step of removing whey.

また、特許文献1には、発酵乳原料液にホエイタンパク質と乳ペプチドとを添加することによって、優れた保形性を有するとともに脆さが少なく、かつ滑らかな食感を有した発酵乳(高タンパク質含有の発酵乳)を製造できることが記載されている。 Further, in Patent Document 1, by adding whey protein and milk peptide to a fermented milk raw material liquid, fermented milk having excellent shape retention and less brittleness and smooth texture (high It is described that protein-containing fermented milk) can be produced.

特開2004−283047号公報JP 2004-283047 A

上記の従来の高タンパク質含有の発酵乳の製造方法では、膜分離処理装置(精密濾過膜、限外濾過膜等)や遠心分離処理装置等の製造設備が必要となり、製造工程も多くなることから、製造費が高くなり、発酵乳そのものの価格も一般に高価となってしまう。また、高タンパク質含有にするために、発酵乳原料液(原料乳)のタンパク質濃度を単に高くしても、固いだけで、舌触り等の風味や食感が悪くなるという問題がある。 In the above-mentioned conventional method for producing fermented milk containing a high amount of protein, manufacturing equipment such as a membrane separation treatment device (microfiltration membrane, ultrafiltration membrane, etc.) or a centrifugal separation treatment device is required, and the number of production steps increases. However, the manufacturing cost becomes high, and the price of the fermented milk itself is generally high. Further, even if the protein concentration of the fermented milk raw material liquid (raw material milk) is simply increased in order to make it contain a high amount of protein, there is a problem that the flavor and texture such as the texture and the like are deteriorated only by being hard.

また、特許文献1には、発酵乳原料液の1質量%程度のタンパク質を配合することによって、発酵乳の物性を改善することが記載されているものの、例えば、原料乳のタンパク質濃度を高くしてから発酵して得られる、高タンパク質含有の発酵乳の製造に必要な条件や、高タンパク質含有の発酵乳の物性等については何ら検討されていない。 Further, although Patent Document 1 describes that the physical properties of fermented milk are improved by adding about 1% by mass of protein of the fermented milk raw material liquid, for example, by increasing the protein concentration of the raw material milk. The conditions necessary for the production of fermented milk with a high protein content obtained by subsequent fermentation and the physical properties of the fermented milk with a high protein content have not been studied at all.

それ故に、本発明は、風味と食感に優れた高濃度の発酵乳を安価に製造できる、発酵乳の製造方法を提供することを目的とする。 Therefore, an object of the present invention is to provide a method for producing fermented milk, which is capable of inexpensively producing high-concentration fermented milk excellent in flavor and texture.

本発明は、タンパク質を高濃度で含む発酵乳の製造方法に関するものである。本発明に係る発酵乳の製造方法では、脱塩脱脂粉乳と、乳タンパク質濃縮物(MPC; Milk
Protein Concentrate)と、ホエイタンパク質濃縮物(WPC; Whey Protein Concentrate)および/またはホエイタンパク質単離物(WPI; Whey Protein Isolate)(以下、単に「ホエイタンパク質濃縮物」ということがある)と、生クリームとを含む原料乳を用い、脱塩脱脂乳と乳タンパク質濃縮物とホエイタンパク質濃縮物の質量の合計を100質量%としたときに、脱塩脱脂粉乳を70〜80質量%、乳タンパク質濃縮物を15〜25質量%、ホエイタンパク質濃縮物を2〜6質量%の割合で配合する。
The present invention relates to a method for producing fermented milk containing a high concentration of protein. In the method for producing fermented milk according to the present invention, desalted skim milk powder and milk protein concentrate (MPC; Milk)
Protein Concentrate), whey protein concentrate (WPC; Whey Protein Concentrate) and/or whey protein isolate (WPI; Whey Protein Isolate) (hereinafter sometimes simply referred to as "whey protein concentrate"), and fresh cream 70% to 80% by mass of desalted skim milk powder, milk protein concentrate, when the total mass of desalted skim milk, milk protein concentrate and whey protein concentrate is 100% by mass, using raw material milk containing In an amount of 15 to 25% by mass and the whey protein concentrate in an amount of 2 to 6% by mass.

本発明に係る製造方法によれば、風味と食感に優れた高濃度の発酵乳を安価に製造することが可能となる。 According to the production method of the present invention, it is possible to inexpensively produce high-concentration fermented milk excellent in flavor and texture.

図1は、実施形態に係る発酵乳の製造工程を示す流れ図である。FIG. 1 is a flow chart showing a manufacturing process of fermented milk according to an embodiment.

本発明の実施形態では、タンパク質原料とクリームとを配合した原料乳を発酵させることにより、タンパク質を高濃度(5〜10質量%)で含む発酵乳(高タンパク質含有の発酵乳)を製造するものである。 In the embodiment of the present invention, fermented milk containing protein at a high concentration (5 to 10% by mass) (fermented milk containing high protein) is produced by fermenting raw material milk containing a protein material and cream. Is.

図1は、実施形態に係る発酵乳の製造工程を示す流れ図である。 FIG. 1 is a flow chart showing a manufacturing process of fermented milk according to an embodiment.

まず、加温した水に、タンパク質原料を溶解または分散させて、タンパク質原料の水溶液を調製する(ステップS1)。タンパク質原料は少なくとも、脱塩脱脂乳の成分と、乳タンパク質濃縮物の成分と、ホエイタンパク質濃縮物の成分とを含む。 First, the protein raw material is dissolved or dispersed in heated water to prepare an aqueous solution of the protein raw material (step S1). The protein raw material contains at least a component of desalted skim milk, a component of a milk protein concentrate, and a component of a whey protein concentrate.

脱塩脱脂乳とは、塩類(ナトリウムやカリウム等のミネラル類)を低減した脱脂乳であり、例えば、ナノ濾過(NF:Nano Filtration)処理を施した脱脂乳が挙げられる。ここで、脱塩脱脂乳とは、脱塩脱脂粉乳、脱塩脱脂濃縮乳等の形態を包含する意味として用いられることがある。 Desalted skim milk is skim milk with reduced salts (minerals such as sodium and potassium), and examples thereof include skim milk that has been subjected to nanofiltration (NF: Nano Filtration) treatment. Here, the desalted skim milk may be used as a meaning that includes forms such as desalted skim milk powder and desalted skim concentrated milk.

乳タンパク質濃縮物は、原料乳の熱安定性を向上させる。また、乳タンパク質濃縮物では、その製造過程において、ナトリウム、カリウム、塩素等が十分に低減(脱塩)されているので、乳タンパク質濃縮物を配合して製造した高濃度の発酵乳では、発酵後に濃縮処理して製造した発酵乳と比べて、塩味を容易に低減することができる。つまり、この塩味の低減によって、実際に得られる発酵乳の風味を、まろやかに調整することが可能となる。ただし、乳タンパク質濃縮物では、ホエイタンパク質濃縮物と比べて、カルシウム含有量が高いため、乳タンパク質濃縮物の配合割合が上記の範囲を超えて高くなりすぎると、カルシウムに起因する苦味が発生しやすくなる。また、乳タンパク質濃縮物の配合割合が上記の範囲を超えて高くなりすぎると、タンパク質に起因する異臭が発生しやすくなる。 Milk protein concentrates improve the heat stability of raw milk. In addition, in the production process of milk protein concentrate, since sodium, potassium, chlorine, etc. are sufficiently reduced (desalted) in the production process, fermented milk with high concentration produced by blending milk protein concentrate is fermented. It is possible to easily reduce the salty taste as compared with the fermented milk produced by being subjected to a concentration treatment later. That is, by reducing the salty taste, it is possible to gently adjust the flavor of the fermented milk that is actually obtained. However, since the milk protein concentrate has a higher calcium content than the whey protein concentrate, if the blending ratio of the milk protein concentrate exceeds the above range and becomes too high, bitterness due to calcium occurs. It will be easier. Further, if the blending ratio of the milk protein concentrate exceeds the above range and becomes too high, an offensive odor due to the protein is likely to occur.

ホエイタンパク質濃縮物は、発酵乳のカードの硬度を向上させる。原料乳に含まれるβ−ラクトグロブリン(β−Lg)やα−ラクトアルブミン(α−La)の含有量が高くなるにつれて、発酵乳のカードの硬度が高くなる。このとき、ホエイタンパク質濃縮物では、脱脂乳や乳タンパク質濃縮物と比べて、β−Lgの含有量やα−Laの含有量が高いため、ホエイタンパク質濃縮物を高濃度で配合することによって、発酵乳の硬度を向上させることができる。ただし、ホエイタンパク質濃縮物の配合割合が上記の範囲を超えて高くなりすぎると、発酵乳の硬度が高くなりすぎて、食感が悪化したり、原料乳の熱安定性が低下しやすくなったり、発酵乳の粒子の粗さが顕著となって、カードの組織が低下しやすくなって脆くなったりする。また、ホエイタンパク質濃縮物では、乳タンパク質濃縮物と比べて、ナトリウム含有量やカルシウム含有量が低いため、ホエイタンパク質濃縮物を配合して、高濃度の発酵乳を製造すると、ナトリウムやカルシウムに起因する塩味や苦味を低減することができる。ただし、ホエイタンパク質濃縮物の配合割合が上記の範囲を下回ると、これらの塩味や苦みを低減することができなくなる。 Whey protein concentrate improves the hardness of the fermented milk curd. The hardness of the curd of fermented milk increases as the content of β-lactoglobulin (β-Lg) or α-lactalbumin (α-La) contained in the raw material milk increases. At this time, in the whey protein concentrate, since the β-Lg content and the α-La content are higher than the skim milk and the milk protein concentrate, by blending the whey protein concentrate in a high concentration, The hardness of fermented milk can be improved. However, if the blending ratio of the whey protein concentrate is too high beyond the above range, the hardness of the fermented milk becomes too high, the texture deteriorates, or the heat stability of the raw material milk tends to decrease. , The graininess of the fermented milk becomes remarkable, and the texture of the curd is apt to deteriorate and becomes brittle. In addition, since whey protein concentrate has a lower sodium content and calcium content than milk protein concentrate, when whey protein concentrate is blended to produce fermented milk of high concentration, it is caused by sodium and calcium. It is possible to reduce saltiness and bitterness. However, if the blending ratio of the whey protein concentrate is less than the above range, the salty taste and bitterness cannot be reduced.

脱塩脱脂乳とホエイタンパク質濃縮物と乳タンパク質濃縮物との配合割合は、脱塩脱脂粉乳と、ホエイタンパク質濃縮物と、乳タンパク質濃縮物の合計を100質量%としたときに、脱塩脱脂粉乳を70〜80質量%、乳タンパク質濃縮物を15〜25質量%、ホエイタンパク質濃縮物を2〜6質量%とする。このとき、脱塩脱脂粉乳と、乳タンパク質濃縮物と、ホエイタンパク質濃縮物との配合割合を上記の範囲とすることによって、原料乳の熱安定性や発酵乳のカードの硬度を向上させつつ、風味や食感を改善することができる
The mixing ratio of desalted skim milk, whey protein concentrate, and milk protein concentrate is such that desalted skim milk powder, whey protein concentrate, and milk protein concentrate are 100% by mass. Milk powder is 70 to 80% by mass, milk protein concentrate is 15 to 25% by mass, and whey protein concentrate is 2 to 6% by mass. At this time, desalted skim milk powder, milk protein concentrate, and by setting the mixing ratio of the whey protein concentrate in the above range, while improving the thermal stability of the raw milk and the hardness of the card of fermented milk, The flavor and texture can be improved.

上述した配合割合の範囲内でも、脱塩脱脂乳と、ホエイタンパク質濃縮物と、乳タンパク質濃縮物の合計を100質量としたときに、脱塩脱脂乳を72〜78質量%、乳タンパク質濃縮物を18〜23質量%、ホエイタンパク質濃縮物を3〜6質量%とすることが好ましく、脱塩脱脂乳を74〜76質量%、乳タンパク質濃縮物を20〜22質量%、ホエイタンパク質濃縮物を3〜5質量%とすることがより好ましく、脱塩脱脂乳を75質量%、乳タンパク質濃縮物を21質量%、ホエイタンパク質濃縮物を4質量%とすることが特に好ましい。尚、ホエイタンパク質濃縮物の配合量を好ましい範囲とすることによって、風味と食感とをさらに向上させることができる。 Even in the range of the above-mentioned mixing ratio, when the total of desalted skim milk, whey protein concentrate, and milk protein concentrate is 100 mass, 72 to 78 mass% desalted skim milk, milk protein concentrate Is preferably 18 to 23% by mass, the whey protein concentrate is 3 to 6% by mass, the desalted skim milk is 74 to 76% by mass, the milk protein concentrate is 20 to 22% by mass, and the whey protein concentrate is It is more preferably 3 to 5% by mass, particularly preferably 75% by mass of desalted skim milk, 21% by mass of milk protein concentrate and 4% by mass of whey protein concentrate. The flavor and texture can be further improved by setting the content of the whey protein concentrate in the preferable range.

次に、タンパク質原料の水溶液に、生クリームを添加(配合)する(ステップS2)。ここで、脱塩脱脂粉乳、乳タンパク質濃縮物、ホエイタンパク質濃縮物を配合して、原料乳に含まれるタンパク質の含有量(濃度)を高くしすぎた場合、カードが硬くなりすぎて、滑らかさが損なわれることがある。そこで、カードの硬さ(軟らかさ)や滑らかさを調整する目的から、生クリームを配合する。この原料の生クリームには、例えば、乳脂肪が35〜50質量%のものを使用することができる。生クリームの配合割合は、タンパク質原料と、生クリームと、水の合計を100質量%としたときに、16〜25質量%とすることができ、17〜25質量%とすることが好ましく、18〜25質量%とすることがより好ましい。生クリームの配合割合が上記の範囲を下回ると、発酵乳のカードに軟らかさや滑らかさを付与しにくくなる。一方、生クリームの配合割合が上記の範囲を超えると、発酵乳のカードに適度な硬さを付与しにくくなる。 Next, fresh cream is added (blended) to the aqueous solution of the protein raw material (step S2). Here, if desalted skim milk powder, milk protein concentrate, whey protein concentrate is blended to make the protein content (concentration) in the raw milk too high, the curd becomes too hard and smooth. May be impaired. Therefore, for the purpose of adjusting the hardness (softness) and smoothness of the curd, a fresh cream is mixed. As the raw cream of this raw material, for example, one having a milk fat content of 35 to 50 mass% can be used. The blending ratio of the fresh cream can be 16 to 25% by mass, and is preferably 17 to 25% by mass, when the total of the protein raw material, the fresh cream and water is 100% by mass. It is more preferable that the content is -25% by mass. When the blending ratio of the fresh cream is below the above range, it becomes difficult to impart softness or smoothness to the fermented milk curd. On the other hand, when the blending ratio of the fresh cream exceeds the above range, it becomes difficult to impart an appropriate hardness to the fermented milk curd.

次に、ホモミキサー等を用いて、原料乳を攪拌して均質化させる(ステップS3)。乳タンパク質濃縮物には、溶解性が低いものがあるため、この工程を設けて均質化処理する。また、生クリームの乳脂肪を微粒化や分散するため、この工程を設けて均質化処理する。ただし、溶解性の高いタンパク質濃縮物を原料として用いる場合には、この工程を省略してもよい。 Next, the raw material milk is stirred and homogenized using a homomixer or the like (step S3). Since some milk protein concentrates have low solubility, this step is provided for homogenization treatment. Further, in order to atomize or disperse the milk fat of the fresh cream, this step is provided and homogenized. However, when a highly soluble protein concentrate is used as a raw material, this step may be omitted.

次に、必要に応じて、均質化処理した後の原料乳のpHを調整してから、原料乳を加熱して所定の殺菌温度と所定の殺菌時間で殺菌処理した(ステップS4)後、原料乳を発酵温度まで冷却する(ステップS5)。原料乳のpHを6.6〜6.8、より好ましくは6.7に調整することによって、原料乳を加熱して殺菌処理するときのタンパク質の変性を抑制して、原料乳の熱安定性を向上させることができる。 Next, if necessary, after adjusting the pH of the raw material milk after the homogenization treatment, the raw material milk is heated and sterilized at a predetermined sterilization temperature and a predetermined sterilization time (step S4), and then the raw material The milk is cooled to the fermentation temperature (step S5). By adjusting the pH of the raw material milk to 6.6 to 6.8, more preferably 6.7, the denaturation of proteins when heating and sterilizing the raw material milk is suppressed, and the heat stability of the raw material milk is suppressed. Can be improved.

次に、冷却した原料乳にスターターを添加(して攪拌)した(ステップS6)後、容器に原料乳を充填し(ステップS7)、所定の発酵温度(と所定の発酵時間)で発酵させる(ステップS8)。そして、原料乳の発酵を終了した後、発酵乳を冷蔵庫で冷却する(ステップS9)。 Next, after adding (and stirring) a starter to the cooled raw material milk (step S6), the container is filled with the raw material milk (step S7) and fermented at a predetermined fermentation temperature (and a predetermined fermentation time) ( Step S8). Then, after the fermentation of the raw material milk is completed, the fermented milk is cooled in the refrigerator (step S9).

本実施形態に係る製造方法では、脱脂乳に高タンパク質原料を配合した原料乳を発酵させるため、従来の高濃度の発酵乳の製造方法のように、通常の発酵乳を膜分離処理や遠心分離処理を施して、ホエイを除去する必要がない。ホエイを除去する濃縮工程が不要となることによって、以下のような利点がある。
(1)膜分離処理装置や遠心分離処理装置等の製造設備やホエイを除去する濃縮工程が不要となるため、発酵乳の製造費を低減することができる。
(2)ホエイを除去する製造方法では、実際に除去したホエイの分だけ発酵乳の質量が少なくなるが、ホエイを除去する濃縮工程を省略する(不要にする)ことによって、発酵乳の損失を削減でき、製品の歩留まりが向上する。
(3)ホエイを除去する濃縮工程を省略することによって、廃棄物となる副産物(ホエイ
)が発生しないので、環境への負荷を軽減できる。
(4)発酵工程後に、濃縮工程を行う場合には、濃縮処理装置等の無菌化操作が必要であるが、濃縮工程が不要となることによって、無菌化操作も不要となる。また、発酵工程後に、発酵乳に何の機械的な処理も行わないので、微生物による汚染のリスクを回避できる。
(5)ホエイを除去する濃縮工程を省略することによって、発酵工程後に、カードの攪拌や破砕などが不要となる。したがって、高濃度のソフトタイプ(糊状)の発酵乳だけでなく、高濃度のセットタイプ(固形状)の発酵乳の製造も可能となる。
In the manufacturing method according to the present embodiment, in order to ferment the raw material milk in which the high-protein raw material is mixed with skim milk, the normal fermented milk is subjected to membrane separation treatment or centrifugation as in the conventional method for producing high-concentration fermented milk. No need to treat to remove whey. Since the concentration step for removing whey is unnecessary, the following advantages can be obtained.
(1) Since the manufacturing equipment such as the membrane separation processing apparatus and the centrifugal separation processing apparatus and the concentration step for removing whey are unnecessary, the manufacturing cost of fermented milk can be reduced.
(2) In the manufacturing method for removing whey, the mass of fermented milk is reduced by the amount of whey actually removed, but by omitting (eliminating) the concentration step for removing whey, the loss of fermented milk is reduced. It can be reduced and the product yield is improved.
(3) By omitting the concentration step for removing whey, by-products (whey), which are wastes, are not generated, and the load on the environment can be reduced.
(4) When the concentration step is performed after the fermentation step, the sterilization operation of the concentration processing device and the like is necessary, but the sterilization operation is not necessary because the concentration step is unnecessary. In addition, since the fermented milk is not subjected to any mechanical treatment after the fermentation process, the risk of microbial contamination can be avoided.
(5) By omitting the concentration step for removing whey, it is not necessary to stir or crush the curd after the fermentation step. Therefore, not only high-concentration soft type (paste-like) fermented milk but also high-concentration set type (solid-state) fermented milk can be produced.

また、原料乳に乳タンパク質濃縮物を配合することによって、原料乳の熱安定性(耐熱性)が向上する。したがって、本実施形態に係る発酵乳の製造方法では、原料乳の殺菌工程において、殺菌処理装置を長時間で連続運転することが可能となり、原料乳を効率的に殺菌処理することができる。 Moreover, the thermal stability (heat resistance) of raw material milk improves by mix|blending a milk protein concentrate with raw material milk. Therefore, in the method for producing fermented milk according to the present embodiment, in the sterilization step of raw material milk, the sterilization apparatus can be continuously operated for a long time, and the raw material milk can be efficiently sterilized.

また、原料乳にホエイタンパク質濃縮物を配合することによって、濃縮工程を行うことなく、発酵乳のカードを硬くすることが可能となり、発酵乳の塩味や苦味を低減して、食味を向上することができる。 Further, by blending the whey protein concentrate in the raw material milk, it becomes possible to harden the curd of the fermented milk without performing a concentration step, reducing the saltiness and bitterness of the fermented milk, and improving the taste. You can

したがって、本実施形態に係る発酵乳の製造方法によれば、風味に優れた高濃度の発酵乳を安価に製造することができる。また、本実施形態に係る製造方法によれば、栄養的に良質なタンパク質を高濃度で含む発酵乳を提供することができる。ここで得られる発酵乳では、酸味が弱いため、調理素材として、様々な料理や調理に使用することができる。また、ここで得られる発酵乳では、発酵終了の段階で、水分の含有量が低く、適度な硬度を有しているため、水切りを行うことなく、様々な料理や調理に使用することができる。 Therefore, according to the method for producing fermented milk according to the present embodiment, it is possible to inexpensively produce high-concentration fermented milk having an excellent flavor. Further, according to the production method of the present embodiment, it is possible to provide fermented milk containing a nutritionally good protein at a high concentration. Since the fermented milk obtained here has a weak acidity, it can be used for various dishes and cooking as a cooking material. Further, the fermented milk obtained here has a low water content at the end of fermentation and has an appropriate hardness, and thus can be used for various dishes and cooking without draining. ..

本実施形態に係る発酵乳の製造方法では、発酵乳のpHを4.6〜4.9、より好ましくは4.6〜4.8、さらに好ましくは4.7〜4.8に調整することによって、発酵乳を製造した直後だけでなく、発酵乳を冷蔵(10℃、2週間)保存した後にも、適度な酸味を維持することができて、風味を特に良好に維持することができる。 In the method for producing fermented milk according to the present embodiment, the pH of fermented milk is adjusted to 4.6 to 4.9, more preferably 4.6 to 4.8, and further preferably 4.7 to 4.8. Thus, not only immediately after the fermented milk is produced, but also after the fermented milk is stored in a refrigerator (10° C., 2 weeks), an appropriate sourness can be maintained, and the flavor can be particularly favorably maintained.

以下、本発明を具体的に実施した実施例を説明する。 Hereinafter, examples in which the present invention is specifically implemented will be described.

まず、タンパク質原料として、脱塩脱脂粉乳と、乳タンパク質濃縮物と、ホエイタンパク質濃縮物を使用し、加温した水に、タンパク質原料を混合して溶解させて、タンパク質原料の水溶液を調製した。ここで、脱塩脱脂粉乳として、ナノ濾過(NF)処理した脱脂粉乳、乳タンパク質濃縮物として、MPC80(DOMO社製)、ホエイタンパク質濃縮物として、WPC80(Arla Foods社製又はTATUA社製)を使用した。 First, desalted skim milk powder, milk protein concentrate, and whey protein concentrate were used as protein raw materials, and the protein raw materials were mixed and dissolved in heated water to prepare an aqueous solution of the protein raw materials. Here, as desalted skim milk powder, nanofiltration (NF)-treated skim milk powder, as a milk protein concentrate, MPC80 (manufactured by DOMO), and as whey protein concentrate, WPC80 (manufactured by Arla Foods or TATAUA). used.

次に、タンパク質原料の水溶液に、生クリーム(乳脂肪分:47質量%)を添加(配合)して混合して、原料乳を調製した後、ホモミキサーを用いて、原料乳を攪拌しながら均質化した。次に、原料乳を加熱して所定の殺菌温度と所定の殺菌時間で殺菌処理した後、原料乳を発酵温度まで冷却した。そして、原料乳にスターターを添加して攪拌した後、容器に原料乳を充填し、所定の発酵温度と所定の発酵時間で発酵させた。原料乳の発酵を終了した後、発酵乳を冷蔵庫で冷却した。 Next, fresh cream (milk fat content: 47 mass%) was added (blended) to the aqueous solution of the protein raw material and mixed to prepare raw material milk, and then the raw material milk was stirred using a homomixer. Homogenized. Next, the raw material milk was heated and sterilized at a predetermined sterilization temperature and a predetermined sterilization time, and then the raw material milk was cooled to the fermentation temperature. Then, after adding a starter to the raw material milk and stirring, the raw material milk was filled in a container and fermented at a predetermined fermentation temperature and a predetermined fermentation time. After the fermentation of the raw material milk was completed, the fermented milk was cooled in a refrigerator.

表1に、実施例1〜3および比較例1〜3に係る発酵乳に用いた原料、配合割合、栄養成分の組成を示す。尚、表1に示したタンパク質原料、生クリーム、水の配合量は、原料乳(タンパク質原料、生クリーム、水)を100質量%としたときの配合割合であり、乳酸菌スターターの配合量は、原料乳を100質量%としたときの配合割合である。また、
表2に、実施例1〜3および比較例1〜3に係る発酵乳の風味および物性(カードの硬さおよび滑らかさ)の官能試験の結果を示す。
Table 1 shows the raw materials used for the fermented milks of Examples 1 to 3 and Comparative Examples 1 to 3, the blending ratio, and the composition of nutritional components. The blending amounts of the protein raw material, fresh cream, and water shown in Table 1 are blending ratios when the raw material milk (protein raw material, fresh cream, water) is 100% by mass, and the blending amount of the lactic acid bacterium starter is It is a blending ratio when the raw material milk is 100% by mass. Also,
Table 2 shows the results of the sensory test of the flavor and physical properties (hardness and smoothness of the curd) of the fermented milks of Examples 1 to 3 and Comparative Examples 1 to 3.

Figure 0006749916
Figure 0006749916

Figure 0006749916
Figure 0006749916

実施例1〜3に係る発酵乳は、脱塩脱脂粉乳、乳タンパク質濃縮物、ホエイタンパク質濃縮物の配合量を上述した好ましい範囲内としたことによって、カードが硬くなりすぎず、滑らかさが悪くならなかった。特に、実施例2および3では、生クリームの配合量を上
述した好ましい範囲としたことによって、ナイフでカットできる適度な硬さと、かき混ぜたときの滑らかさを両立することができた。これに対して、比較例1〜3に係る発酵乳は、乳たんぱく質濃縮物の配合量が好ましい範囲内の上限を超えたことによって、カードが硬くなり、滑らかさが悪くなった。なお、実験結果を省略するが、発酵乳のpHを4.6〜4.9に調整して製造すると、発酵乳を製造した直後だけでなく、発酵乳を冷蔵(10℃、2週間)保存した後にも、適度な酸味が維持されて、風味が特に良好に維持されることを確認した。
Fermented milk according to Examples 1 to 3, desalted skim milk powder, milk protein concentrate, by setting the amount of whey protein concentrate in the above-described preferred range, the curd does not become too hard, the smoothness is poor. did not become. Particularly, in Examples 2 and 3, by setting the blending amount of the fresh cream within the above-described preferable range, it was possible to achieve both appropriate hardness that can be cut with a knife and smoothness when stirred. On the other hand, in the fermented milks of Comparative Examples 1 to 3, the curd became hard and the smoothness deteriorated because the blending amount of the milk protein concentrate exceeded the upper limit within the preferable range. In addition, although the experimental result is omitted, when the pH of the fermented milk is adjusted to 4.6 to 4.9 to produce the fermented milk, the fermented milk is stored not only immediately after the production but also in the refrigerated (10°C, 2 weeks) It was confirmed that the sourness was maintained moderately even after the treatment, and the flavor was particularly well maintained.

本発明は、栄養的に良質なタンパク質を高濃度で含む発酵乳を提供することができる。 INDUSTRIAL APPLICABILITY The present invention can provide fermented milk containing a nutritionally good quality protein at a high concentration.

Claims (2)

脱塩脱脂乳と、乳タンパク質濃縮物と、ホエイタンパク質濃縮物とを含む水溶液を調製し、調製した水溶液に生クリームを添加して原料乳を調製し、調製した原料乳を発酵させることにより、タンパク質を高濃度で含む発酵乳を得るための発酵乳の製造方法であって、
前記脱塩脱脂乳と前記乳タンパク質濃縮物と前記ホエイタンパク質濃縮物の合計を100質量%としたときに、
前記脱塩脱脂乳を70〜80質量%、
前記乳タンパク質濃縮物を15〜25質量%、
前記ホエイタンパク質濃縮物を2〜6質量%
となるように、前記脱塩脱脂乳と、前記乳タンパク質濃縮物と、前記ホエイタンパク質濃縮物を配合することを特徴とする、発酵乳の製造方法。
Desalted skim milk, a milk protein concentrate, and an aqueous solution containing a whey protein concentrate are prepared, raw cream is prepared by adding fresh cream to the prepared aqueous solution, and by fermenting the prepared raw milk, A method for producing fermented milk to obtain fermented milk containing a high concentration of protein,
When the total of the desalted skim milk, the milk protein concentrate, and the whey protein concentrate is 100% by mass,
70 to 80 wt% of the desalting de Aburachichi,
15-25% by mass of the milk protein concentrate,
2-6% by weight of the whey protein concentrate
As a, the a desalting de Aburachichi, said milk protein concentrate, the whey is characterized by blending the protein concentrate, the manufacturing method of fermented milk.
前記発酵乳の全質量に占めるタンパク質の割合が5〜10質量%であることを特徴とする、請求項1に記載の発酵乳の製造方法。 The method for producing fermented milk according to claim 1, wherein the proportion of protein in the total weight of the fermented milk is 5 to 10% by mass.
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