JP6747672B2 - Spectroscopic measuring device and spectroscopic measuring method - Google Patents

Description

The present invention relates to a spectroscopic measurement device and a spectroscopic measurement method.

Patent Document 1 discloses a spectroscopic analysis device for performing DNA identification using spectral data. In Patent Document 1, a sample containing a DNA fragment labeled with a fluorescent substance is irradiated with excitation light. Then, the observed spectrum is measured by spectroscopically measuring the fluorescence generated in the sample. In Patent Document 1, the sample is irradiated with light from a light source via an optical fiber.

International Publication No. 2014/045481

In such a spectroscopic device, it is necessary to appropriately guide the fluorescence to the spectroscope. Specifically, if the fluorescence spreads in the spectroscopic direction of the spectroscope and enters, the accuracy of spectroscopic measurement will decrease.

An object of the present invention is to provide a spectroscopic measurement device and a spectroscopic measurement method capable of performing accurate spectroscopic measurement.

A spectroscopic measurement device according to an aspect of the present invention, a chip provided with a plurality of flow channels through which a sample migrates, a light source that generates light to be irradiated to the sample in the flow channel, and each of the flow channels, A plurality of optical fibers having a lens that collects light from the sample and an incident end surface on which the light collected by the lens is incident, the emitting end surface being arranged along a first direction. A plurality of bundled optical fibers, a spectroscope that disperses light from the emission end face in a second direction orthogonal to the first direction, and a two-dimensional array light that detects the light dispersed by the spectroscope And a detector.
A spectroscopic measurement method according to an aspect of the present invention includes a step of irradiating light from a light source onto a sample that migrates through a plurality of channels provided in a chip, a step of collecting light from the sample with a lens, Causing the light condensed by the lens to enter the incident end faces of the plurality of optical fibers; emitting the light from the emission end faces of the optical fibers arranged along the first direction; The method further comprises a step of dispersing the light from the emission end face in a second direction orthogonal to the first direction, and a step of detecting the light dispersed by the spectroscope by a two-dimensional array photodetector. is there.

According to the present invention, it is possible to provide a spectroscopic measurement device and a spectroscopic measurement method capable of performing accurate spectroscopic measurement.

It is a figure which shows typically the structure of the spectroscopic analysis apparatus concerning embodiment of this invention. It is a side view which shows typically the structure of the optical system unit provided under the microchip. It is a top view which shows arrangement|positioning of the incident end surface of the some optical fiber with respect to a capillary. It is a figure which shows arrangement|positioning of the output end surface of several optical fiber with respect to the incident surface of a spectroscope. It is a top view which shows arrangement|positioning of the 1st and 2nd optical fiber group with respect to a spectroscope. It is a top view which shows the structure of an example of an optical system unit. FIG. 9 is a top view showing the arrangement of the incident end surface of the optical fiber with respect to the capillary in the spectroscopic analysis device according to the second embodiment. FIG. 8 is a diagram showing the arrangement of the emission end faces of a plurality of optical fibers with respect to the incident face of the spectroscope in the spectroscopic analysis device according to the second embodiment. 8 is a top view showing the arrangement of optical fiber groups with respect to a spectroscope in the spectroscopic analysis device according to the second embodiment.

Embodiments of the present invention will be described with reference to the accompanying drawings. The embodiments described below are examples of the present invention, and the present invention is not limited to the following embodiments. In this specification and the drawings, constituent elements having the same reference numerals indicate the same elements.

Embodiment 1.
In the present embodiment, DNA base sequence analysis is performed using a plurality of fluorescent substances having different emission wavelengths. Specifically, DNA is extracted from human cells. Then, the DNA fragment is amplified by polymerase chain reaction (PCR) and labeled with a fluorescent substance. As the fluorescent substance, for example, 5-FAM, JOE, NED, ROX or the like can be used. Of course, the fluorescent substance to be labeled is not particularly limited. Here, a plurality of fluorescent substances having different peak wavelengths are used as labels. And different bases are labeled with different fluorescent substances.

Separate fluorescently labeled PCR products are applied to the capillaries and electrophoresed in a gel. When a voltage is applied by electrophoresis, the moving speed varies depending on the size of the DNA fragment. The smaller the number of bases, the longer the migration distance, so that the DNA fragments can be size-separated. When the PCR product in the capillary is irradiated with the excitation light from the light source, the fluorescent substance emits fluorescence. Then, the fluorescence generated from the fluorescent substance is spectroscopically measured to obtain the observed spectrum data. Observed spectrum data is acquired for each size. Then, by analyzing these observed spectrum data, the DNA of a specific sequence can be quantified and the DNA test can be performed.

In this embodiment, the spectroscopic analysis device is described as being used for DNA appraisal, but the use of the spectroscopic analysis device according to the present embodiment is not limited to DNA appraisal. It can be applied to a spectroscopic analyzer that analyzes a spectrum of fluorescence generated from a sample labeled with a substance by a fluorescent probe. For example, it is possible to analyze nucleic acids, proteins and the like. The spectroscopic analysis device can also be used for identification of substances. Further, the substance contained in the sample may be labeled with a labeling substance other than the fluorescent substance. As the labeling substance, it is preferable to use a substance having a shifted peak wavelength of light.

A spectroscopic analysis device (spectroscopic measurement device) according to the present invention will be described with reference to FIG. FIG. 1 is a diagram showing the configuration of the spectroscopic analysis device. The spectroscopic analysis device includes a microchip 20, an optical system unit 30, and a processing unit 16. The microchip 20 includes an injection part 11 and a capillary 12. The optical system unit 30 includes a light source 13, a spectroscope 14, a detector 15, an optical fiber 31, a light projecting optical fiber 32, and a lens 34. Here, analysis is performed using capillary electrophoresis.

A PCR product containing a DNA fragment labeled with a fluorescent substance is injected into the injection unit 11. Here, a sample DNA fragment is labeled with a plurality of fluorescent substances. For example, fluorescent substances such as 5-FAM, JOE, NED, and ROX are used depending on the base sequence of the DNA fragment. Of course, the type and number of fluorescent substances for labeling are not particularly limited.

The injection part 11 communicates with the capillary 12 on the microchip 20. Electrodes (not shown) are arranged at both ends of the capillaries 12 provided on the microchip 20, and a voltage is applied. The capillary 12 and the injection part 11 are filled with an electrophoretic medium such as agarose gel. Therefore, the migration speed becomes slower depending on the number of bases of the DNA fragment, and the DNA fragment is size-separated.

The light source 13 irradiates the medium in the capillary 12 with light. As the light source 13, for example, an argon ion laser light source that emits excitation light having a wavelength of 488 nm or 514.5 nm can be used. The light from the light source 13 enters the light projecting optical fiber 32. The light projecting optical fiber 32 is made of a material through which light can propagate.

The light emitted from the light source 13 propagates through the light projecting optical fiber 32 and enters the capillary 12. Here, eight lanes of capillaries 12 are provided in parallel on the microchip 20. A light projecting optical fiber 32 is provided in each of the eight lane capillaries 12. When the eight lanes of the capillary 12 are irradiated with the excitation light, the fluorescent substance that labels the DNA fragment in the capillary 12 emits fluorescence. The fluorescence generated by the fluorescent substance becomes the observation light.

The 8-lane capillaries 12 provided on the microchip 20 are identified as channels CH1 to CH8. Each of the channels CH1 to CH8 serves as a channel through which the sample migrates. Excitation light is applied to the samples in the respective channels CH1 to CH8. In the present embodiment, the description will be made assuming that the microchip 20 is provided with the 8-lane capillaries 12, but the microchip 20 may be provided with a plurality of capillaries 12.

The fluorescence generated by the fluorescent substance in the sample is condensed by the lens 34 and enters the optical fiber 31. The fluorescence propagates through the optical fiber 31 and enters the spectroscope 14. For example, the spectroscope 14 has a prism, a diffraction grating, or the like, and disperses the fluorescence. That is, the fluorescence is spatially dispersed according to the wavelength. The fluorescence spatially dispersed by the spectroscope 14 enters the detector 15. Therefore, the fluorescence emitted from the fluorescent substance becomes the observation light observed by the detector 15.

The detector 15 is, for example, a photodetector such as a CCD (Charge Coupled Device) sensor, and pixels are arranged along the dispersion direction (spectral direction). Specifically, the detector 15 is a two-dimensional array photodetector in which pixels are arranged along the X direction and the Y direction. The X direction and the Y direction are directions orthogonal to each other. The X direction is the direction corresponding to the spectral direction, and the Y direction is the direction orthogonal to the spectral direction. Therefore, fluorescence of different wavelengths is detected for each pixel arranged in the spectral direction.

The detector 15 detects fluorescence from the fluorescent substance labeled with the DNA fragment and outputs a detection signal to the processing unit 16. For example, the spectroscope 14 and the detector 15 measure a spectrum in the wavelength range of 200 to 800 nm. The number of data included in the spectrum data is a value according to the spectral performance of the spectroscope 14. Of course, the wavelength range that can be spectroscopically measured by the spectroscope 14 and the detector 15 is not particularly limited. It can be appropriately set according to the fluorescent substance used as the label and the wavelength of the excitation light.

The detector 15 outputs the light intensity at each wavelength in the observable wavelength range as observation spectrum data to the processing unit 16. The number of data included in the observed spectrum data is a value according to the spectral performance of the spectroscope 14 and the like.

The processing unit 16 is an information processing device such as a personal computer and performs processing according to a control program. Specifically, the processing unit 16 stores an analysis program that analyzes the observed spectrum data output from the detector 15. Then, the processing unit 16 executes processing according to the analysis program. Based on the observed spectrum data output from the detector 15, the processing unit 16 analyzes a plurality of substances contained in the sample. Thereby, the concentration of the DNA fragment is obtained. By doing so, the DNA test can be performed. As the analysis method, for example, the matrix calculation or the integrated value calculation in the window disclosed in Patent Document 1 can be used.

The configuration of the optical system unit 30 will be described in detail with reference to FIGS. FIG. 2 is a side view schematically showing the configuration of the optical system unit 30. FIG. 3 is a top view schematically showing the arrangement of the incident end surface 31a of the optical fiber 31 with respect to the capillary 12 of CH1. FIG. 4 is a diagram schematically showing the arrangement of the emission end surface 31b of the optical fiber 31 with respect to the incident surface 14a of the spectroscope 14. FIG. 5 is a diagram schematically showing the arrangement of the spectroscope 14 and a plurality of fiber groups.

As shown in FIG. 2, an optical system unit 30 is arranged below the microchip 20. As described above, the microchip 20 is provided with the capillary 12 whose longitudinal direction is the migration direction. The light projecting optical fiber 32 irradiates the capillary 12 with the excitation light L1 obliquely from below. The light projecting optical fiber 32 may be provided for each capillary 12. That is, when there is an 8-channel capillary 12, eight projecting optical fibers 32 are provided.

A lens 34 is provided immediately below the position irradiated with the excitation light L1 from the light projecting optical fiber 32. That is, the lens 34 is arranged directly below the irradiation location of the excitation light L1. The lens 34 arranged directly below the capillary 12 collects the fluorescence L2 generated by the fluorescent substance in the sample flowing through the capillary 12. The optical fiber 31 is arranged immediately below the lens 34. Therefore, the fluorescence L2 generated in the sample is condensed by the lens 34 and is incident on the incident end surface 31a of the optical fiber 31. By disposing the lens 34 immediately below the capillary 12, it is possible to increase the amount of the fluorescent light L2 entering the optical fiber 31. The fluorescence L2 generated in various directions can be efficiently detected. The diameter of the optical fiber 31 is about submicron to several microns.

The fluorescence L2 incident on the incident end surface 31a of the optical fiber 31 propagates in the optical fiber 31. As shown in FIG. 3, ten optical fibers 31 are provided for the capillary 12 of one channel. The incident end faces 31a of the ten optical fibers 31 are arranged along the direction orthogonal to the migration direction. By doing so, the fluorescence from the sample at the same migration distance can be propagated through the plurality of optical fibers 31. Therefore, the amount of fluorescence detection light can be increased. The ten optical fibers 31 may be provided in the detection window as described later.

Here, the diameter of the incident end face 31 a of the optical fiber 31 is smaller than the width of the capillary 12. In FIG. 3, among the 10 optical fibers 31 provided in the capillary 12 of 1CH, three optical fibers 31 are located immediately below the capillary 12, and the remaining seven are arranged to extend beyond the width of the capillary 12. R. The fluorescence L2 collected by the lens 34 (not shown in FIG. 3) mainly enters the three tubes located immediately below the capillary 12. The width of the region where the ten optical fibers 31 are provided is wider than the width of the capillary 12. By doing so, the optical fiber 31 can be arranged immediately below the capillary 12 even if a displacement occurs when the microchip 20 is attached. That is, it is possible to eliminate the influence on the positional deviation in the direction orthogonal to the migration direction. Therefore, a stable light amount can be obtained.

As described above, ten optical fibers 31 are provided for the one-channel capillary 12. Further, the microchip 20 is provided with an 8-channel capillary. Therefore, the optical system unit 30 is provided with 80 optical fibers 31. The number of optical fibers 31 corresponding to one channel and the number of channels of the capillaries 12 provided in the microchip 20 are not limited to the above numbers.

As shown in FIG. 2, the emission end face 31b of the optical fiber 31 is arranged toward the spectroscope 14. That is, the emission end face 31b of the optical fiber 31 and the incident face of the spectroscope 14 are arranged to face each other. The exit end face 31b of the optical fiber 31 and the entrance face of the spectroscope 14 are preferably arranged in parallel.

The fluorescence L3 emitted from the emission end surface 31b of each optical fiber 31 is emitted to the incident surface of the spectroscope 14. The spectroscope 14 has a prism or the like as described above, and disperses the fluorescence L3 according to the wavelength. Therefore, the spectroscope 14 disperses the fluorescence L3 in a predetermined spectral direction. The fluorescence L4 emitted from the emission surface of the spectroscope 14 is received by the detector 15. The spectroscope 14 disperses the fluorescence L3 in one of the pixel arrangement directions of the detector 15 (X direction). Therefore, the fluorescence L4 having different wavelengths is detected by the pixels at different positions in the X direction.

As shown in FIG. 4, the plurality of optical fibers 31 are bundled so that the emission end faces 31b of the plurality of optical fibers 31 are aligned in the direction (first direction) orthogonal to the spectral direction. That is, the emission end faces 31b of the plurality of optical fibers 31 are arranged in the direction orthogonal to the spectral direction of the spectroscope 14. In FIG. 2, the up-down direction in the plane of the drawing is the arrangement direction (first direction) of the emission end faces 31b. The arrangement direction (first direction) of the emission end faces 31b of the plurality of optical fibers 31 coincides with the other of the pixel arrangement directions (Y direction).

With such an arrangement, accurate spectroscopic measurement can be performed. That is, since the emission end face 31b of the optical fiber 31 is sufficiently small, it is possible to reduce the spread of the fluorescence L31 incident on the incident face 14a of the spectroscope 14. Therefore, the fluorescence can be appropriately dispersed according to the wavelength, and accurate spectroscopic measurement can be performed.

Further, it is preferable to align the output end faces 31b of the plurality of optical fibers 31 so as to be arranged in one plane parallel to the incident face 14a of the spectroscope 14. By doing so, the angular distribution of the fluorescence L3 entering the spectroscope 14 can be made uniform. This allows accurate spectroscopic measurement. The exit end side of the optical fiber 31 may be cut in order to align the exit end face 31b of the optical fiber 31.

As described above, each of CH1 to CH8 is provided with ten optical fibers 31. Here, as shown in FIGS. 4 and 5, the 40 optical fibers 31 corresponding to the capillaries 12 of CH1 to CH4 are set as a first optical fiber group 41, and the 40 optical fibers 31 corresponding to the capillaries 12 of CH5 to CH8 are arranged. The optical fiber 31 is a second optical fiber group 42. In each of the first optical fiber group 41 and the second optical fiber group 42, 40 optical fibers 31 are bundled.

In the optical fibers 31 included in the first optical fiber group 41, the emission end faces 31b are arranged along the first direction (the vertical direction in FIG. 4, the direction perpendicular to the paper surface of FIG. 5). Similarly, in the optical fibers 31 included in the second optical fiber group 42, the emission end faces 31b are arranged along the first direction.

The positions of the emission end surface 31b of the first optical fiber group 41 and the emission end surface 31b of the second optical fiber group 42 are displaced in the second direction orthogonal to the first direction which is the arrangement direction of the emission end surfaces 31b. .. In other words, the emission end faces 31b of the 80 optical fibers 31 are arranged in two rows. Then, in the second direction orthogonal to the first direction, the first row corresponding to the first optical fiber group 41 and the second row corresponding to the second optical fiber group are arranged at intervals. Has been done.

As shown in FIG. 5, the fluorescence L3 emitted from the first optical fiber group 41 is incident on the incident surface 14a of the spectroscope 14. Then, the fluorescence L3 is dispersed in the X direction by the spectroscope 14, and is emitted from the emission surface 14b of the spectroscope 14 as the fluorescence L4. The fluorescence L5 emitted from the second optical fiber group 42 is incident on the incident surface 14a of the spectroscope 14. Then, the fluorescence L5 is dispersed in the X direction by the spectroscope 14 and emitted from the emission surface 14b of the spectroscope 14 as the fluorescence L6. Then, the fluorescence L6 enters the detector 15. Therefore, in the X direction, the fluorescence L4 and the fluorescence L6 are incident on different positions of the detector 15.

In this way, the emission end faces 31b of the two or more optical fibers 31 included in the first optical fiber group 41 are arranged along the first direction. Further, the emission end faces 31b of the two or more optical fibers 31 included in the second optical fiber group 42 are arranged along the first direction. Then, as shown in FIG. 5, the emission end face 31 b of the first optical fiber group 41 and the second optical fiber group 42 are arranged so that the fluorescence L4 and the fluorescence L6 do not overlap with each other on the light receiving surface of the detector 15. The emission end face is arranged so as to be displaced in the second direction.

Therefore, on the light receiving surface of the detector 15, the fluorescence L4 and the fluorescence L6 are detected by the pixels at different positions (X addresses) in the X direction. The detector 15 can detect the fluorescence L4 and the fluorescence L6 independently. That is, the detector 15 detects the fluorescence L4 and the fluorescence L6 in different pixels. Thereby, the spectrum of the fluorescence L4 and the spectrum of the fluorescence L6 can be separately measured. By doing so, the light from the 80 optical fibers 31 can be simultaneously measured. That is, spectroscopic measurement can be performed simultaneously on the 8-channel capillaries 12.

Further, in each of the first and second optical fiber groups 41 and 42, the emission end face 31b is arranged along the first direction. Therefore, the fluorescence from different optical fibers 31 enters different pixels in the Y direction. Therefore, the light from the capillaries 12 of the respective channels can be separately spectroscopically measured. By doing so, the spectrum can be measured accurately, so that the accuracy of DNA test can be improved.

A specific arrangement example of the optical system unit 30 having the above configuration will be described with reference to FIG. FIG. 6 is a top view of the optical system unit 30. In FIG. 6, the horizontal direction is the migration direction of the sample.

As shown in FIG. 6, the optical system unit 30 has a fixed block 35. The fixing block 35 is provided with grooves and holes for fixing the optical fiber 31 and the light projecting optical fiber 32. In FIG. 6, the light projecting optical fiber 32 is attached to the fixed block 35 from the right side of the fixed block 35. The light projecting optical fiber 31 is fixed to the fixed block 35 via a connector or the like. Here, eight light projecting optical fibers 32 are provided, and each of them irradiates the capillary 12 of each channel with excitation light. As described above, each of the light projecting optical fibers 32 irradiates the capillary 12 with the excitation light from obliquely below.

Further, the fixed block 35 is provided with eight detection windows 36. The detection window 36 corresponds to each channel of the capillary 12. Each detection window 36 is provided next to the light projecting optical fiber 32. The detection window 36 is arranged immediately below the capillary 12. A lens 34 (not shown in FIG. 6) for collecting fluorescence is arranged above each detection window 36. For example, the detection window 36 is formed by providing a hole in the fixed block 35.

Since ten optical fibers 31 are provided in the one-channel capillary 12 as shown in FIG. 3, ten optical fibers 31 are arranged in one detection window 36. In the plane of the paper of FIG. 6, the incident end faces 31a of the ten optical fibers 31 are arranged vertically. Therefore, the incident end faces 31a of 80 optical fibers 31 for 8 channels are arranged in a line. In FIG. 6, the four detection windows 36 on the lower side 4 correspond to the capillaries 12 of CH1 to CH4, and the four detection windows 36 on the upper side correspond to the capillaries 12 of CH5 to CH8.

Then, 40 optical fibers 31 corresponding to the capillaries 12 of CH1 to CH4 are bundled to form a first optical fiber group 41. Specifically, the 40 optical fibers 31 are twisted so that the emission end faces 31b of the 40 optical fibers 31 are aligned in the direction perpendicular to the paper surface. That is, the plurality of twisted optical fibers 31 are arranged so that the arrangement direction of the incident end faces 31b (vertical direction in the paper) and the arrangement direction of the emission end faces (direction perpendicular to the paper) are orthogonal to each other. Becomes In this way, the bundle of two or more optical fibers 31 included in the first optical fiber group 41 is twisted.

Similarly, 40 optical fibers 31 corresponding to the capillaries 12 of CH5 to CH8 are bundled to form a second optical fiber group 42. Specifically, the 40 optical fibers 31 are twisted so that the emission end faces 31b of the 40 optical fibers 31 are aligned in the direction perpendicular to the paper surface. That is, the plurality of optical fibers 31 twisted so that the arrangement direction of the incident end faces 31 b (vertical direction in the paper) and the arrangement direction of the emission end faces (direction perpendicular to the paper) are orthogonal to each other are the second optical fiber group 42. Become. In this way, the bundle of two or more optical fibers 31 included in the second optical fiber group 42 is twisted. For example, by arranging the emission end face 31b of the optical fiber 31 in a slit (not shown) provided in the fixed block 35, two or more optical fibers 31 can be arranged in a line.

Then, the fluorescence L3 emitted from the first optical fiber group 41 and the fluorescence L5 emitted from the second optical fiber group 41 are incident on the incident surface 14a of the spectroscope 14. The spectroscope 14 has a triangular prism. Then, the spectroscope 14 wavelength-disperses the fluorescence. The fluorescences L4 and L6 emitted from the emission surface 14b of the spectroscope 14 enter the detector 15, respectively. With this configuration, since the bottom surface of the triangular prism of the spectroscope 14 can be arranged as the lower surface, the installation and adjustment of the optical system are facilitated.

By using such a configuration, it is possible to spectroscopically measure the fluorescence from the sample flowing through the 8-channel capillary 12. That is, the fluorescence from each optical fiber 31 is detected by different pixels. Therefore, the fluorescence from each capillary 12 can be accurately spectroscopically measured.

Embodiment 2.
The second embodiment is different from the first embodiment in the arrangement of the optical fiber 31. The configuration other than the arrangement of the optical fiber 31 is the same as that of the first embodiment, and thus the description thereof is omitted. The configuration of the optical system unit 30 according to the second embodiment is shown in FIGS. FIG. 7 is a top view showing the arrangement of the incident end surface 31 a of the optical fiber 31 with respect to the capillary 12. FIG. 8 is a diagram showing the arrangement of the emission end faces of the plurality of optical fibers with respect to the incident face 14 a of the spectroscope 14. FIG. 9 is a top view showing the arrangement of the first optical fiber group 41 with respect to the spectroscope 14.

In the second embodiment, as shown in FIG. 7, one optical fiber 31 is provided for each capillary 12 of one channel. Fluorescence generated by the sample in the one-channel capillary 12 propagates in one optical fiber 31. The diameter of the incident end surface 31a of the optical fiber 31 is larger than the width of the capillary 12.

Further, in the present embodiment, the capillary 12 has 5 channels. Therefore, the microchip 20 is provided with five capillaries 12. One optical fiber 31 is provided for the one-channel capillary 12. Therefore, in this embodiment, five optical fibers 31 are provided between the capillary 12 and the spectroscope 14. Similar to the first embodiment, the fluorescence generated in the sample in the capillary 12 enters the incident end surface 31a of the optical fiber 31 via the lens 34.

Then, as shown in FIG. 8, the emission end faces 31b of the five optical fibers 31 are arranged in a line. Similar to the first embodiment, the array direction (first direction) of the emission end faces 31b is orthogonal to the spectral direction of the spectroscope 14. In this way, the emission end faces 31b of the optical fibers 31 corresponding to the two or more capillaries 12 are arranged along the first direction. In the present embodiment, the emission end faces 31b of all the optical fibers 31 are arranged in a line. Therefore, the second optical fiber group 42 is not provided, and only the first optical fiber group 41 is provided.

Further, in the second embodiment, as shown in FIG. 9, a slit 38 may be arranged between the emitting end face 31b and the incident face 14a of the spectroscope 14. The slit 38 has an opening 38a along the first direction (direction perpendicular to the paper surface of FIG. 9). Then, the slit 38 limits the passage of the fluorescence L2 incident on the outside of the opening 38a. Even when the emission end face 31b of the optical fiber 31 has a large diameter, the spread of the fluorescence L3 on the incident face 14a of the spectroscope 14 can be reduced. As described above, by providing the slit 38 in the optical system unit 30, more accurate spectroscopic measurement can be performed.

As described above, in the second embodiment, one optical fiber 31 is provided for the capillary 12 of one channel, and the emission end face 31b of the optical fiber 31 corresponding to the capillaries 12 of two or more channels is the first. Are lined up in a row along the direction of. Therefore, also in the configuration shown in the second embodiment, the same effect as in the first embodiment can be obtained. That is, accurate spectroscopic measurement can be performed. Note that the configuration of Embodiment 1 and the configuration of Embodiment 2 can be combined as appropriate.

Although the present invention has been described with reference to the exemplary embodiments, the present invention is not limited to the above. Various modifications that can be understood by those skilled in the art can be made to the configuration and details of the present invention within the scope of the invention.

This application claims the priority on the basis of Japanese application Japanese Patent Application No. 2015-71672 for which it applied on March 31, 2015, and takes in those the indications of all here.

11 injection part 12 capillary 13 light source 14 spectroscope 14a incident surface 14b emission surface 15 detector 16 processing part 20 microchip 30 optical system unit 31 optical fiber 31a incident end face 31b emission end face 32 projection optical fiber 34 lens 35 fixed block 36 Detection window 38 Slit 41 First optical fiber group 42 Second optical fiber group

Claims (10)

A chip having a plurality of channels through which a sample migrates,
A light source for generating light to be applied to the sample in the channel,
A lens that is provided in each of the flow paths and that collects light from the sample,
A plurality of optical fibers having an incident end surface on which the light condensed by the lens is incident, the plurality of optical fibers bundled so that the emitting end surfaces are arranged along the first direction,
A spectroscope that disperses light from the emission end face in a second direction orthogonal to the first direction;
A two-dimensional array photodetector for detecting the light dispersed by the spectroscope, comprising :
Two or more optical fibers are provided for one of the flow paths,
The emission end faces of the optical fibers corresponding to the two or more flow paths are arranged along the first direction,
A spectroscopic measurement device in which the incident end faces of two or more optical fibers are arranged in a direction orthogonal to the migration direction of the flow path. A chip having a plurality of channels through which a sample migrates,
A light source for generating light to be applied to the sample in the channel,
A lens that is provided in each of the flow paths and that collects light from the sample,
A plurality of optical fibers having an incident end surface on which the light condensed by the lens is incident, the plurality of optical fibers bundled so that the emitting end surfaces are arranged along the first direction,
A spectroscope that disperses light from the emission end face in a second direction orthogonal to the first direction;
A two-dimensional array photodetector for detecting the light dispersed by the spectroscope,
The spectroscopic measurement device , wherein the incident end faces of the plurality of optical fibers are arranged along a direction orthogonal to the first direction . Two or more optical fibers are provided for one of the flow paths,
The spectroscopic measurement device according to claim 2 , wherein the emission end faces of the optical fibers corresponding to two or more flow paths are arranged along the first direction. The plurality of optical fibers includes a first optical fiber group and a second optical fiber group,
Emission end faces of two or more of the optical fibers included in the first optical fiber group are arranged along the first direction,
The emission end faces of two or more of the optical fibers included in the second optical fiber group are arranged along a first direction,
The light emitted from the first optical fiber group and dispersed by the spectroscope and the light emitted from the second optical fiber group and emitted by the spectroscope are dispersed by the detector. 4. The emission end face of the first optical fiber group and the emission end face of the second optical fiber group are arranged so as to be offset from each other in the second direction so as not to overlap on the light receiving surface. The spectroscopic measurement device according to claim 1. One optical fiber is provided for one flow path,
The spectroscopic measurement device according to claim 2 , wherein the emission end faces of the optical fibers corresponding to two or more flow paths are arranged along the first direction. Irradiating a sample that migrates through a plurality of channels provided on the chip with light,
Collecting light from the sample;
Injecting the condensed light into the incident end faces of the plurality of optical fibers;
Emitting light from an emission end face of the optical fibers arranged along a first direction;
Splitting the light from the emission end face in a second direction orthogonal to the first direction,
Detecting the dispersed light by the two-dimensional array light detector, a spectroscopic measurement method with a,
Two or more optical fibers are provided for one of the flow paths,
The emission end faces of the optical fibers corresponding to the two or more flow paths are arranged along the first direction,
The incident end faces of two or more optical fibers are arranged in a direction orthogonal to the migration direction of the flow path.
The spectroscopic measurement method . Irradiating a sample that migrates through a plurality of channels provided on the chip with light,
Collecting light from the sample;
Injecting the condensed light into the incident end faces of the plurality of optical fibers;
Emitting light from an emission end face of the optical fibers arranged along a first direction;
Splitting the light from the emission end face in a second direction orthogonal to the first direction,
Detecting the dispersed light with a two-dimensional array photodetector,
The spectroscopic measurement method, wherein the incident end faces of the plurality of optical fibers are arranged along a direction orthogonal to the first direction. Two or more optical fibers are provided for one of the flow paths,
The spectroscopic measurement method according to claim 7 , wherein the emission end faces of the optical fibers corresponding to the two or more flow paths are arranged along the first direction. The plurality of optical fibers includes a first optical fiber group and a second optical fiber group,
Emission end faces of two or more of the optical fibers included in the first optical fiber group are arranged along the first direction,
The emission end faces of two or more of the optical fibers included in the second optical fiber group are arranged along a first direction,
The light emitted from the first optical fiber group and spectrally separated from the light emitted from the second optical fiber group and emitted and spectrally separated do not overlap on the light receiving surface of the detector. The spectroscopy according to any one of claims 6 to 8, wherein the emission end face of the first optical fiber group and the emission end face of the second optical fiber group are arranged so as to be displaced in the second direction. Measuring method. One optical fiber is provided for one flow path,
The spectroscopic measurement method according to claim 7 , wherein the emission end faces of the optical fibers corresponding to the two or more flow paths are arranged along the first direction.

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