JP6727881B2 - Sample loading plate - Google Patents

Description

The present invention relates to a sample loading plate for loading a sample.

A matrix assisted laser desorption/ionization (MALDI) method is known as one of mass spectrometry ionization methods capable of rapidly and accurately diagnosing pathogens and bacteria.

The MALDI method uses a substance (matrix) that easily absorbs laser light and is easily ionized in order to analyze an analyte (hereinafter referred to as a sample) that hardly absorbs laser light or is easily damaged by laser light. This is a method in which the sample is mixed in advance and the sample is ionized by irradiating it with laser light.

In a mass spectrometer by the MALDI method, generally, an analyte and a matrix are mixed in advance and liquefied by a solvent (hereinafter referred to as a sample, which is a liquid at the time of dropping, but also a crystallized product by drying it). A metal plate called a target plate for loading a sample (hereinafter referred to as a sample loading plate) is placed in the apparatus, and the sample loaded on the sample loading plate is irradiated with laser light for a predetermined time. Desorb and ionize the sample. At this time, a voltage is applied to the metal sample loading plate, and an electric field is applied to the deionized and ionized sample, thereby facilitating the deionized and ionized sample to fly toward the acceleration electrode.

The sample loading plate has a plurality of sample loading areas (hereinafter referred to as sample loading spots) for loading samples, and a plurality of samples to be measured are dropped on a predetermined sample loading spot to be dried (crystallized). In this state, the sample is placed in the mass spectrometer, and the sample loading plate is moved to irradiate a plurality of samples with laser light.

In the MALDI analysis method, such crystals are deposited in the sample loading spot as uniformly as possible, the analyte is appropriately deionized and ionized, and the voltage applied to the sample loading plate effectively applies an electric field to the sample to appropriately Acceleration is important and many proposals have been made for these analytical techniques.

Regarding the improvement of crystallization or ionization of the sample in the sample loading spot, for example, the proposal shown in Patent Document 1 discloses that the sample loading spot has a central portion having an electrically conductive surface and a margin (surrounding) portion made of a hydrophobic mask. The sample dropped on the sample loading spot is crystallized and accumulated in a ring shape in the hydrophobic margin portion by the halo effect. The crystal ring formed in the margin portion is efficiently irradiated with laser light for ionization.

Further, the proposal shown in Patent Document 2 is a groove in which a conductive interference layer is provided on a substrate having an insulating property so as to exhibit a color different from that of the substrate, a hydrophobic film is formed on the surface, and a sample loading spot is formed. Is provided to expose the substrate, and the dropped sample is retained in the sample loading spot (hereinafter referred to as an anchor effect) for crystallization and ionization.

Japanese Patent Publication No. 2006-525525 WO2015/019861

However, in the conventional technique disclosed in Patent Document 1, although the crystal ring of the sample formed in the margin portion of the sample loading spot is irradiated with laser light to perform efficient measurement, the central part having electrical conductivity is used. Since the margin part is an insulating film for the part, the conductivity is not sufficient and the voltage applied to the sample loading plate against the deposited crystal ring does not effectively apply an electric field to the sample and the sample charges up. However, there is a problem that proper ionization is hindered.

Further, in the conventional technique disclosed in Patent Document 2, the visibility of the sample loading spot is secured by forming a groove exposing the substrate at the boundary between the outer side and the inner side of the sample loading spot, but an insulating substrate is used. Therefore, it is necessary to partially connect the membranes in order to ensure electrical continuity between the outer side and the inner side of the sample loading spot, and therefore it is necessary to form the groove intermittently. Insufficient connection between the film on the outside of the sample loading spot and the film on the inside of the sample loading spot may cause charge-up at the time of analysis, which may lead to decrease in analysis sensitivity.

An object of the present invention is to solve the above problems, to solve the above problems, and to provide a sample loading plate having good visibility and continuity of a sample loading spot.

In order to achieve the above-mentioned subject, the composition of the present invention is as follows.
A sample loading plate used for mass spectrometry of MALDI method, comprising at least one sample loading spot for loading a sample on a substrate, wherein an outer peripheral portion of the sample loading spot is a hydrophobic surface, The island, which is the inner region, is a hydrophilic surface and is characterized in that the color of the island is different from that of the outer peripheral portion of the sample loading spot.

It is characterized in that the outer peripheral portion and the island are formed with different film configurations.

At least one metal film among the film configurations formed on the outer peripheral portion and the island is continuous and conductive.

The substrate is characterized by being made of ceramics.

The hydrophilic layer is characterized by being composed of a metal film formed on the substrate or an optical multilayer film formed on the metal film.

As a result, the sample can be accurately dropped on the sample loading spot, and the electrical connection between the outside and the inside of the sample loading spot is surely ensured.

According to the present invention, it is possible to provide a sample loading plate in which the sample visibility in the sample loading spot is good and the conductivity is excellent.

The sample can be accurately dropped on the sample loading spot, and the dropped sample surely spreads in the sample loading spot. As a result, it is possible to provide a sample loading plate which has good sample visibility in mass spectrometry, a high anchor effect for the dropped sample, and good conduction between the outside and the inside of the sample loading spot.

Further, an arbitrary color can be created by the first metal film and the optical multilayer film laminated on the substrate. As a result, a color difference between the outer side and the inner side of the sample loading spot can be easily created, the visibility of the loaded sample can be enhanced, and the efficiency of the sample dropping operation is improved. Further, since it is possible to easily see the sample loading spot and various marks to be formed, the work management of the sample becomes easy. In addition, by storing sample loading plates of various colors and color-coding, sample storage and management becomes easy. Further, in the mass spectrometry of the MALDI method, the voltage applied through the peripheral portion of the sample loading plate can surely conduct electricity to the sample in the sample loading spot by the first metal film or the metal film in the optical multilayer film. ..

I-I' cross-sectional view of a sample loading spot in Example 1 of the present invention I-I' cross-sectional view of a sample loading spot in Example 2 of the present invention The top view which shows the sample mounting plate of this invention. The figure explaining the structural example of the 1st metal film and optical multilayer film laminated|stacked in the sample mounting plate of this invention. Sectional drawing which illustrates typically the coloring principle by the interference of an optical multilayer film. Partial cross-sectional view illustrating a state in which a sample is loaded on a sample loading spot in the present invention. Schematic diagram for explaining the operation of the mass spectrometer Process drawing explaining the manufacturing method which concerns on the sample mounting plate of embodiment of this invention.

Embodiments of the present invention will be described below with reference to FIGS.

The sample loading plate is placed on a mass spectrometer by the MALDI method (see FIG. 7 described later), and is used to load a sample on a sample loading spot and analyze the mass. The embodiments for carrying out the invention shown below exemplify a sample loading plate and a manufacturing method thereof for embodying the idea of the present invention, and the present invention is specified by the method and configuration described below. Not a thing. In particular, the manufacturing method and the shapes, materials, relative arrangements, etc. of the members and the manufacturing methods described in the embodiments are not intended to limit the scope of the present invention unless otherwise specified. In addition, the sizes and shapes of members shown in the drawings, positional relationships, and film layers to be formed may be exaggerated for easy understanding.

[Description of Sample Loading Plate of Embodiment: FIGS. 1 to 4]
First, the configuration of the sample loading plate according to the embodiment of the present invention will be described with reference to FIG. FIG. 3 is a plan view of the sample loading plate viewed from the surface on which the sample is loaded.

The substrate 1 of the sample loading plate 100 is an insulating, substantially rectangular flat plate having an outer shape of about 50 mm×40 mm, and the sample loading plate 100 is made of, for example, a material such as Al ₂ O ₃ (alumina). Can be made using. Further, a cutout portion is provided like the lower side for positioning, for example. Further, the flatness of the sample loading plate 100 has an accuracy of 30 μm or less. The outer shape, thickness, etc. are not particularly limited as long as they meet the specifications of the mass spectrometer. The sample loading plate may be surface-finished by a lapping process or a polishing process in order to secure the flatness.

A plurality of substantially circular sample loading spots 10 are formed on the sample loading plate 100. In this embodiment, there are a total of 96 pieces, which are 8 pieces vertically and 12 pieces horizontally. Here, the number of sample loading spots 10 is not limited to this, and is determined so as to meet the specifications of the mass spectrometer.

The sample loading plate 100 has column address marks 30 (for example, 1 to 9, X to Z) indicating the positions of the sample loading spots 10, row address marks 40 (for example, A to H), and a serial for managing the sample loading plate. The number 50, the bar code 60, etc. can be formed. These address marks, serial numbers, bar codes, etc. are not limited to these and may be added or deleted as necessary. Here, the method of forming the address mark, the serial number, and the bar code is not particularly limited, but a processing method by laser marking is preferable.
Next, two examples of the cross-sectional structure of the sample loading spot 10 will be described.

FIG. 1 is a cross-sectional view taken along a cutting line II′ passing through the center of the sample loading spot 10 in the first embodiment. An enlarged view of the sample loading spot is also shown at the top so that the two-dimensional positional relationship can be seen. The sample loading spot 10 is composed of an island 21, and an outer peripheral portion 22 is provided around the outer periphery of the island 21. The surface of the island 21 is hydrophilic, and the surface of the outer peripheral portion 22 has a structure in which a hydrophobic film is formed.

Here, the first metal film 2M is first formed on one surface of the substrate 1. Next, an optical multilayer film 2A is formed by laminating it on the first metal film 2M. The optical multilayer film 2A is made of a dielectric film or a second metal film, and the type of film and the number of layers are not particularly limited, and are formed in the order of 2d, 2c, 2b, and 2a, for example. Further, the hydrophobic film 12 is formed on the optical multilayer film. The first metal film 2M and the optical multilayer film 2A are formed by a film forming method such as vacuum deposition or sputtering. The hydrophobic film 12 is similarly formed by a film forming method such as vacuum deposition or sputtering, but a method such as dip coating in which it is immersed in a liquid and slowly pulled up to form a film is also possible. The metal film in the first metal film 2M or the optical multi-layer film is a film continuous to the island 21, the outer peripheral portion 22 and the peripheral portion 20 of the sample loading plate to ensure electrical conductivity. ing.

In the first embodiment, the island 21 of the sample loading spot 10 is exposed by Ni, which is the first metal film, by peeling off the optical multilayer film 2A. Since Ni is a gray color close to white, there is a difference in color from the dark blue color of the optical multilayer film 2A, and therefore the visibility of the sample loading spot is good. At the same time, the first metal film is continuously conducted from the island 21 to the outer peripheral portion 22 and further to the edge portion 20 of the sample loading plate. This is an important point as a sample loading plate for MALDI.

Next, the film and substrate cross-sectional structure of the sample loading plate 100 will be described with reference to FIG.

FIG. 4 shows a sectional configuration of the sample loading plate 100. In the cross-sectional structure shown in FIG. 4, Al ₂ O ₃ is used for the substrate 1. The first metal film 2M laminated on the substrate 1 uses Ni as a material and has a film thickness of about 300 nm (1 nm=0.000001 mm). Next, the first layer 2d constituting the optical multilayer film 2A uses Al ₂ O _{3 and} has a film thickness of about 80 nm. The second layer 2c is made of Ti and has a film thickness of about 10 nm. The third layer 2b is made of SiO _{2 and} has a film thickness of 90 nm. The fourth layer 2a is made of Ti and has a film thickness of about 10 nm. With such a configuration, the surface of the sample loading plate 100 can be dark blue in the wavelength range of visible light. A hydrophobic film 12 made of C (carbon), F (fluorine), Si (silicon), or the like is formed on the optical multilayer film 2A. The hydrophobic film 12 has a thickness of, for example, 2 to Since the thickness is as thin as about 3 nm, there is little influence on the conductivity and color of the inner surface of the sample loading spot 10.

As described above, by appropriately combining the first metal film laminated on the substrate 1 and the optical multilayer film 2A, an arbitrary reflection characteristic (coloring) utilizing optical interference can be obtained. The optical multilayer film 2A may be a dielectric film as well as a metal film mixed as shown in FIG.

Regarding the hydrophilicity, since the surface of the island 21 is the first metal film 2M, it is hydrophilic, the outer peripheral portion 22 is the hydrophobic film 12, and a structure capable of exerting an anchoring effect such that the sample is retained on the island 21. Has become.

FIG. 2 is a cross-sectional view taken along a cutting line II′ passing through the center of the sample loading spot 10 in the second embodiment.
The difference from the first embodiment is that in the second embodiment, the island 21 of the sample loading spot 10 has a 2c layer exposed by peeling off the 2a layer and the 2b layer which are a part of the optical multilayer film 2A. is there. The other points are the same as those of the first embodiment.

In Example 2, the surface exposed to the island 21 is a color different from the dark blue color developed by the 2c layer and the 2d layer of the optical multilayer film and the first metal film, so that all layers of the optical multilayer film 2A and Since there is a difference in color from the dark blue color developed by the first metal film, the visibility of the sample loading spot is good. At the same time, the first metal film and the 2c layer in the optical multilayer film 2A are continuously conducted from the island 21 to the outer peripheral portion 22 and further to the edge portion 20 of the sample loading plate. This is an important point as the sample loading plate for MALDI, as in the first embodiment.

Regarding the hydrophilicity, since the surface of the island 21 is the 2c layer of the optical multilayer film 2A, it is hydrophilic, and the outer peripheral portion 22 is the hydrophobic film 12, and a structure capable of exerting an anchoring effect such that the sample is retained on the island 21. Has become.

[Explanation on Coloring and Visibility of Sample Loading Plate: FIG. 5]
Next, coloring of the sample loading plate will be described with reference to FIG. FIG. 5 is a schematic diagram for explaining light interference when an optical multilayer film is formed on a substrate.

In FIG. 5, for the sake of explanation, the substrate 1 has, for example, laminated dielectric films 2a, 2b, 2c, and 2d as optical multilayer films. An arbitrary reflection characteristic (coloring) can be obtained by adjusting the material (refractive index), thickness, and the number of layers of each layer, but here, the description will be limited to the principle using a schematic diagram. (In general, a dielectric film having a high refractive index and a dielectric film having a low refractive index are paired and alternately laminated with a thickness of ¼ wavelength to cause the reflected waves from the interfaces of the layers to interfere with each other due to the interference of light. It is said that they can be added to obtain a highly efficient reflection function.)

The incident light P entering the optical multilayer film from the air layer 90 first generates a reflected wave 2aR at the interface between the air and the dielectric film 2a. Similarly, reflected waves 2bR, 2cR, 2dR, and 1R are generated at the interfaces of the layers. The reflections from the respective interfaces are added together to form a reflected wave R. The reflected wave R can obtain an arbitrary reflection characteristic (coloring) by changing the material (refractive index), film thickness, and number of film layers of each layer. Various reflection characteristics can be obtained by mixing a metal film with the dielectric film. In this embodiment, a metal film is used for the intermediate layer 2c and the uppermost layer 2a.

As a result of selecting the specific film quality and film thickness as shown in FIG. 4 based on this principle, the reflection characteristic of the sample loading plate 100 in Example 1 is the visible light wavelength region W (about 380 nm to about 780 nm). Then, the reflectance is low as a whole, but there is a peak at which the wavelength is small, that is, dark blue light is reflected abundantly, and the surface of the outer peripheral portion 22 looks dark blue.

In Example 1 and Example 2, the island 21 has a color other than navy blue because all or part of the optical multilayer film 2A is peeled off, and due to the difference in color from the navy blue of the outer peripheral portion 22, The visibility of the island 21 brings about an effect.

[Explanation of Analysis Operation by Mass Spectrometer: FIGS. 6 and 7]
Next, the operation of performing mass spectrometry of the sample will be described with reference to FIGS. Here, the sample loading plate and the part related to the ionization of the sample will be mainly described, and the other parts will be described only in principle and will not be described in detail. FIG. 6 shows a state in which the sample 200 is loaded on the above-described sample loading plate 100, and FIG. 7 is a schematic view showing a state in which the sample loading plate loaded with the sample 200 is placed on the mass spectrometer 300.

FIG. 6 is a cross-sectional view showing a state in which the sample 200 in which the analyte and the matrix are mixed and liquefied with the solvent is dropped on the sample loading spot to evaporate the solvent and to be dried. A predetermined amount of the sample 200 is dropped on the island 21 (see FIGS. 1 and 2) of the sample loading spot 10 by an unillustrated device. The dropped sample 200 tries to spread radially due to gravity and surface tension. The sample 200 reaches the outer peripheral portion 22 while spreading radially. Since the surface of the island 21 is highly hydrophilic and the outer peripheral portion is hydrophobic, the sample 200 hardly spreads on the outer peripheral portion 22 and stays and is retained on the island 21 (anchor effect). ..

Then, after the loading of the samples 200 to be analyzed is completed, each sample 200 is dried in that state. At this time, since the sample loading spot 10 on the sample loading plate 100 has a high anchor effect for holding the sample 200 in the spot, it is difficult to move even if vibrated, it can be stably held during dropping, and the work can be facilitated. ..

Next, FIG. 7 shows a schematic diagram of the mass spectrometer 300, in which the sample loading plate 100 on which the sample 200 is loaded is placed on the mass spectrometer 300 and fixed by a fixing unit (not shown). Actually, the sample 200 loaded on a plurality of spots has a mechanism capable of moving in the X and Y directions and stopping each sample at a predetermined position. Will be described.

In the mass spectrometer 300 shown in FIG. 7, the sample loading plate 100 is placed on the left side and is detachably fixed by a clamp part (not shown). In addition, the voltage can be applied to the sample loading plate 100 from a voltage applying unit (not shown). Further, a laser light source 220 for irradiating the sample 200 with a laser beam 220a, an ion accelerator 230 for accelerating the ionized analytes (200a, 200b, 200c) that are separated from the sample 200 by the laser beam irradiation and are ionized. An ion trap unit 231 that traps ions, a mass separation unit 232 that forms a flight space of ions to perform mass separation of each ion, and an ion detection unit 240 that detects each mass-separated and arrived ion in time series are provided. ing.

Here, it is assumed that the ion polarity of the analyte is positive (plus charge). When the mass spectrometry starts, the laser light source 220 irradiates the sample 200 to be measured with the laser light 220a for a predetermined time. At the same time, a positive voltage V1 is applied to the first metal film 2M of the sample mounting plate 100 from a voltage applying unit (not shown), and the positive voltage is effectively applied to the sample with respect to the sample 200. At the same time, a negative voltage V2 is applied to the first grid of the ion trap section 231. In Example 1, in addition to the first metal film 2M, the metal film 2c in the optical multilayer film 2A also exhibits conductivity.

At this time, the matrix contained in the sample 200 is vaporized together with the analyte, and the analyte is desorbed and ionized. Then, since a positive voltage V1 is applied to the analyte and an electric field having a downward gradient is generated toward the ion trap portion 231 to which the negative voltage V2 is applied, the desorbed and ionized analyte is ion-accelerated. At 230, it is accelerated toward the ion trap portion 231. In this way, the desorbed and ionized analyte is sent from the ion trap unit 231 to the mass separation unit (flight space) 232, and is separated due to the difference in mass during the flight, resulting in a time difference of 200c, 200b, and 200a. The ions arrive at the ion detector in order. Then, the data detected by the ion detector 240 is analyzed by an analyzer (not shown), and mass analysis is performed on the analyte. As a result, the sample can be identified at high speed and with high accuracy.

[Effect of Embodiment]
As described above, according to the present invention, it is possible to provide a sample loading plate in which the visibility of the sample in the sample loading spot is good and the conductivity is excellent, and a manufacturing method thereof.

The sample can be accurately dropped on the sample loading spot, and the dropped sample surely spreads in the sample loading spot. As a result, it is possible to provide a sample loading plate which has good sample visibility in mass spectrometry, a high anchor effect for the dropped sample, and good conduction between the outside and the inside of the sample loading spot.

Further, an arbitrary color can be created by the first metal film and the optical multilayer film laminated on the substrate. As a result, a color difference between the outer side and the inner side of the sample loading spot can be easily created, the visibility of the loaded sample can be improved, and the efficiency of the sample dropping operation is improved. Further, since it is possible to easily see the sample loading spot and various marks to be formed, the work management of the sample becomes easy. In addition, by storing sample loading plates of various colors and classifying them into different colors, sample storage and management becomes easier. Further, in the mass spectrometry of the MALDI method, the voltage applied through the peripheral portion of the sample loading plate can surely conduct electricity to the sample in the sample loading spot by the first metal film or the metal film in the optical multilayer film. ..

In addition, although the example in which the ceramic Al ₂ O ₃ is used for the substrate has been described in the embodiment, the present invention is not limited to this, and other ceramic materials, composite materials of porcelain and ceramics, glass, Si, plastic, and the like may be used. Further, although an example in which Ni, Ti, or Al is used as the metal film of the first metal film 2M or the optical multilayer film 2A has been described, the present invention is not limited to this, and other metals such as chromium and gold may be used. Moreover, although an example in which Al ₂ O ₃ , SiO ₂ , and TiO ₂ is used as the material of the dielectric film has been described, the present invention is not limited to this, and other dielectric materials such as MgO, MgF ₂ , and ZrO ₂ may be used.

In the present embodiment, the first metal film 2M, the optical multilayer film 2A, and the hydrophobic film 12 are formed on the substrate 1, but other hydrophilic film or the like may be formed on the surface of the substrate 1, for example. Well, it is expected to increase the effects such as visibility.

Further, in this embodiment, the metal film and the optical multilayer film are formed only on one surface of the substrate, but it is more convenient to form the metal film and the optical multilayer film on both surfaces of the substrate depending on the method of film formation. In some cases, a metal film and an optical multi-layer film may be formed on both surfaces of the substrate, or either the metal film or the optical multi-layer film may be formed on the surface on which the sample is not loaded, and further, in a planar manner. It may be formed partially.

[Description of Method of Manufacturing Sample Loading Plate 100: FIG. 8]
Next, a method for manufacturing the sample loading plate 100 of this embodiment will be described with reference to FIG. FIG. 8 is a process diagram showing a method for manufacturing the sample loading plate 100.

[Description of manufacturing method: FIG. 8]
In FIG. 8, main steps 310 to 370 of the method for manufacturing the sample loading plate 100 will be shown and described. Unless otherwise specified in each step, it is natural to perform general work such as transfer, inspection, cleaning, drying, and annealing necessary for each step, and the description thereof will be omitted.

[Substrate receiving process: 310]
First, in the substrate receiving step 310, the flatness and surface roughness of the substrate 1 are inspected to confirm that they have predetermined flatness and surface roughness.

[Substrate surface processing step (enlarged view): 320]
Next, in the substrate surface processing step 320, the substrate 1 is subjected to lapping processing or polishing processing so as to have a predetermined substrate thickness, surface roughness, and flatness. The main inspection items in this step are the surface roughness and flatness of the substrate.

[First metal film forming step (enlarged view): 330]
Next, in the first metal film forming step 330, the first metal film 2M is formed. For example, Ni is formed to a thickness of 300 nm, for example, using a film forming method such as vacuum deposition or sputtering. At this time, in order to obtain a film as uniform as possible, the irradiation direction of the film-forming particles is preferably the vertical direction (see the broken line arrow).

[Optical multilayer film forming step (enlarged view): 340]
Next, in the optical multilayer film forming step 340, the optical multilayer film 2A is laminated. For example, the 2d layer, 2c layer, 2b layer, and 2a layer shown in FIG. 4 are sequentially formed by a film forming method such as vacuum deposition or sputtering.

[Hydrophobic film forming step: 350]
Next, in the hydrophobic film forming step 350, the hydrophobic film 12 is laminated on the surface of the optical multilayer film 2A formed in the previous step. For example, a water repellent material containing, for example, C (carbon), F (fluorine), or Si (silicon) or a composite water repellent material thereof having a thickness of 2 nm is formed by a film forming method such as vacuum deposition. Form.

[Hydrophobic film removal step: 370]
Finally, in the hydrophobic film removal step 370, the hydrophobic film 12 formed on the island 21 of the sample loading spot 10 is peeled off. For example, a mask 15 (detailed description thereof is omitted) is formed on the outside of the sample loading spot 10 by a processing method such as plasma etching, and the hydrophobic film 12 is peeled off. The mask 15 has a function of opening the island 21 and protecting the other outer peripheral portion 22 from plasma.

By the manufacturing method described above, it is possible to provide the manufacturing method of the sample loading plate having a desired reflection color due to the optical multilayer film on the surface of the substrate. In addition, it is possible to provide a method of manufacturing a sample loading plate that has good sample visibility and a high anchor effect for the dropped sample.

Although the various embodiments of the sample loading plate and the manufacturing method thereof have been described in detail above, the present invention is not limited to such embodiments and manufacturing methods, and in terms of detailed configuration, material, and quantity, Any change, addition, or deletion can be made without departing from the spirit of the invention. That is, changes and omissions can be made within the scope of the contents described in each claim of the above-mentioned sample loading plate and manufacturing method.

1 Substrate 2A Optical Multilayer Film 2M First Metal Film 10 Sample Loading Spot 12 Hydrophobic Film 15 Mask 20 Edge of Sample Loading Plate 21 Island (of Sample Loading Spot) 22 Outer Peripheral (of Sample Loading Spot) 30 Column Address Mark 40 line address mark 50 Serial number 60 Bar code 100 Sample loading plate 200 Samples 200a, 200b, 200c Ionized analyte 220 Laser light source 220a Laser light 230 Ion accelerator 231 Ion trap 232 Mass separation (flight space)
240 ion detector 300 MALDI mass spectrometer

Claims (2)

A sample loading plate used for mass spectrometry of MALDI method, comprising at least one sample loading spot for loading a sample on a substrate, comprising:
Formed on the substrate is at least one metal film that electrically connects the sample loading spot and the edge of the sample loading plate,
The island, which is the inner region of the sample loading spot, is a hydrophilic surface formed by at least one metal film formed on the substrate,
The outer peripheral portion of the sample loading spot is a hydrophobic surface formed with a film configuration different from that of the island,
A sample loading plate, characterized in that the color of the outer periphery of the sample loading spot and the island are different. The sample loading plate according to claim 1, wherein the substrate is made of ceramics.

Images