JP6664714B2 - Method for reducing infectivity of abnormal prion protein and pharmaceutical composition for prevention and treatment of prion disease - Google Patents

Description

  The present invention relates to a method for reducing infectivity of abnormal prion protein and a pharmaceutical composition for preventing and treating prion disease.

The prion protein is composed solely of the protein, and all mammals have only one prion protein gene, and the expressed prion protein plays an important role in the brain nervous system.
Abnormal prion protein (PrPres: scrapie prion protein) is a pathologically transmitted prion protein in which a normal prion protein (PrPc: cellular prion protein) present in a normal animal is mutated and causes prion disease. I have. The abnormal prion protein has the same amino acid sequence (primary structure) as the normal prion protein but has a different three-dimensional structure.

Prion disease is a disease in which the central nervous system is affected by the accumulation of abnormal prion protein.Prion disease has been observed in many mammals, including humans, including cows, goats, sheep, pigs, monkeys, mice, and rats. Have been. In addition, prion disease is transmitted and transmitted by prions across animal species.
The infectivity of the abnormal prion protein is not eliminated by high-pressure steam sterilization and dry heat sterilization, is not denatured and inactivated by drugs such as formalin and alcohol, and cannot be degraded by proteolytic enzymes. Therefore, research on effective treatments for prion disease is being conducted. For example, Non-Patent Document 1 reports a method of inhibiting conversion of a normal prion protein to an abnormal prion protein.

On the other hand, it is said that water containing mineral components may have effects such as soil modification, plant growth, decomposition of harmful chemicals, deodorization, air purification, etc. Facilities for producing water containing and mineral containing water have been developed.
The present inventors immerse a conductive wire and a mineral-imparting material (A) coated with an insulator in water, pass a DC current through the conductive wire, and apply the DC current to water around the conductive wire in the same manner as the DC current. Means for generating a water flow in the direction, applying ultrasonic vibration to the water to form a raw material mineral aqueous solution (A), and irradiating the formed raw material mineral aqueous solution (A) with far-infrared rays to produce the mineral-containing water (A). ) Has been developed (see Patent Literature 1).
In addition, the present inventors connect a mineral-containing water producing apparatus (A), a plurality of water-flow containers filled with different types of mineral-imparting materials (B), and a plurality of the water-flow containers in series. A water supply path, a detour water path connected to the water supply path in a state of being in parallel with each of the plurality of water flow containers, and a water flow switching valve provided at a branch between the water supply path and the detour water path, respectively. (See Patent Document 2). In addition, the inventors of the present invention used the mineral-functional water production facility disclosed in Patent Document 2 and conducted repeated studies focusing on the types and mixing ratios of the mineral-imparting materials. It is reported that water exhibits an excellent control effect on single-celled organisms, viruses, and the like (Patent Document 3). Further, they have found that mineral water produced under other raw materials and production conditions has a body activating effect (Patent Document 4).

Japanese Patent No. 4817817 JP 2011-56366 A Japanese Patent No. 5864010 PCT / JP2015 / 076268

Biochem Biophys Res Commun. 2003 Jun 6; 305 (3): 548-51.

As mentioned above, various mineral-containing waters have been reported in the past, but the effects of mineral-containing water have not been scientifically proven in many cases, and the true action of mineral-containing water has not yet been reported. There are many parts that have not been clarified. For this reason, conventional mineral-containing water may not have the actual effect while claiming its effect, or may have an effect that is insufficient for practical use or has poor effect reproducibility. Not a few.
The mineral functional water produced by using the apparatus reported in Patent Document 2 cannot be said to be able to reliably produce a mineral functional water exhibiting a target beneficial effect. In particular, the types and mixing ratios of the raw materials (mineral-imparting materials) of the mineral components used in the mineral-containing water producing apparatuses (A) and (B) are complicatedly involved, and what kind of mineral-imparting materials are used, The fact is that it has not always been clear whether a mineral-functional water exhibiting various effects can be obtained. Until now, the effect of reducing the infectivity of abnormal prion proteins by mineral water has not been confirmed.

  Under these circumstances, an object of the present invention is to provide a method for reducing the infectivity of abnormal prion protein which contributes to the prevention and treatment of prion disease by reducing the infectivity of abnormal prion protein as a new use of mineral functional water, and prion disease To provide a pharmaceutical composition for the prophylactic treatment of

  The present inventors have conducted intensive studies to solve the above problems, and as a result, have found that specific mineral functional water has an effect of reducing the infectivity of abnormal prion protein, and have reached the present invention.

That is, the present invention relates to the following inventions.
<1> A method for reducing the infectivity of an abnormal prion protein, which comprises bringing an abnormal prion protein into contact with mineral-functional water containing a mineral component that emits electromagnetic waves to reduce the infectivity of the abnormal prion protein.
<2> The method according to <1>, wherein the mineral component is a mineral component that emits an electromagnetic wave including a terahertz wave.
<3> The mineral-functional water containing the electromagnetic radiation-emitting mineral component is composed of the mineral-containing water (A) formed in the following step (1) and the mineral-containing water (B) formed in the following step (2). <1> or <2>, which is a mineral functional water obtained by mixing at a ratio of 1: 5 to 1:20 (weight ratio).
Step (1):
A conductive wire covered with an insulator, a plant material consisting of a plant of the family Asteraceae and a plant of the family Rosaceae, and a woody plant comprising at least one woody plant selected from maple, birch, pine and cedar; And a mineral-imparting material (A) containing a plant material, immersed in water, conducting a direct current to the conductive wire, generating a water flow in the same direction as the direct current in the water around the conductive wire, A step of forming a raw mineral aqueous solution (A) by applying ultrasonic vibration to water, and then irradiating the raw mineral aqueous solution (A) with far infrared rays (wavelength 6 to 14 μm) to form a mineral-containing water (A) And
The amount of the mineral-imparting material (A) added to water is 10 to 15% by weight, and the current value and the voltage value of the direct current conducted to the conductive wire are in the range of 0.05 to 0.1 A and 8000 to 8600 V, respectively. And
The mineral imparting material (A)
As the plant material, wild thistle (leaves, stems and flowers), mugwort (leaves and stems), and camellia (leaves and stems) are respectively 8 to 12% by weight and 55 to 65% by weight. , 27-33% by weight, dried and crushed Asteraceae plant after being dried and crushed, and
17-23% by weight, 8-12% by weight, 65-75% by weight of wild rose (leaf, flower), radish (leaf and stem), and raspberry (leaf, stem and flower), respectively Using a dry and crushed Rosaceae plant that was mixed and dried at a ratio of
A plant material (A1) obtained by mixing the dry and crushed Asteraceous plant and the dry and crushed Rosaceae plant in a ratio of 1: 0.8 to 1: 1.2 (weight ratio);
As the woody plant raw materials, maple (leaves and stems), birch (leaves, stems, and bark), cedars (leaves, stems, and bark) are each 22 to 28% by weight, A woody plant raw material (A2) comprising a dry and crushed product obtained by mixing at a ratio of 22 to 28% by weight and 45 to 55% by weight, drying, and then crushing the mixture.
Step of being a mineral-imparting material (A ′) obtained by mixing the plant material (A1) and the woody plant material (A2) in a weight ratio of 1: 2.7 to 1: 3.3.

Step (2):
Mineral-containing water (B) filled with different kinds of inorganic mineral-imparting materials, and water is passed through six water-passing containers from a first water-passing container to a sixth water-passing container connected in series, and mineral-containing water (B) is a step of forming
In the six water containers,
The mineral-imparting material (B1) in the first water passage container contains 65 to 75% by weight, 12.5 to 17.5% by weight, and 12.5 to 17.5% by weight of limestone, fossil coral, and shell, respectively. mixture,
The mineral-imparting material (B2) in the second water passage container contains limestone, fossil coral, shells, and activated carbon in an amount of 37 to 43% by weight, 12.5 to 17.5% by weight, 37 to 43% by weight, and 2.5%, respectively. A mixture comprising ~ 7.5% by weight;
The mineral-imparting material (B3) in the third water container contains limestone, fossil coral, and shells at 75 to 85% by weight, 12.5 to 17.5% by weight, and 2.5 to 7.5% by weight, respectively. mixture,
The mineral-imparting material (B4) in the fourth water passage container contains 85 to 95% by weight, 2.5 to 7.5% by weight, and 2.5 to 7.5% by weight of limestone, fossil coral, and shell, respectively. mixture,
The mineral-imparting material (B5) in the fifth water passage container contains limestone, fossil coral, and shells in an amount of 75 to 85% by weight, 7.5 to 12.5% by weight, and 7.5 to 12.5% by weight, respectively. mixture,
A mixture in which the mineral imparting material (B6) in the sixth water passage container contains 55 to 65% by weight, 27 to 33% by weight, and 7.5 to 12.5% by weight of limestone, fossil coral, and shells;
<5> The method according to <1>, wherein the mineral component is a mineral component derived from mineral functional water CAC-717 manufactured by Riken Technosystem Co., Ltd.
<6> The method according to <1>, wherein the mineral component is a mineral component derived from a mineral functional water A20ACA-717 manufactured by Riken Technosystem Co., Ltd.

<6> A pharmaceutical composition for preventing and treating prion disease, comprising a mineral functional water containing a mineral component that emits electromagnetic waves as an active ingredient.
<7> The pharmaceutical composition according to <6>, wherein the mineral component is a mineral component derived from mineral functional water CAC-717 manufactured by Riken Technosystem Co., Ltd.
<8> The pharmaceutical composition according to <6>, wherein the mineral component is a mineral component derived from a mineral functional water A20ACA-717 manufactured by Riken Technosystem Co., Ltd.

  According to the present invention, there is provided a method for reducing infectivity of abnormal prion protein, which contributes to the prevention and treatment of prion disease by reducing the infectivity of abnormal prion protein, and a pharmaceutical composition for prevention and treatment of prion disease.

It is a block diagram showing a schematic structure of a mineral functional water production facility. FIG. 2 is a schematic diagram of a mineral-containing aqueous solution producing unit that forms a part of the mineral-containing water (A) producing apparatus that constitutes the mineral-functional water producing facility shown in FIG. 1. FIG. 3 is a partially omitted sectional view taken along line AA of FIG. 2. FIG. 3 is a perspective view showing a storage container for a mineral-imparting material (A) used in the raw-material-mineral-water-solution producing means shown in FIG. 2. FIG. 3 is a schematic diagram showing a reaction state in the vicinity of a conductive wire in the raw material aqueous mineral solution production means shown in FIG. 2. FIG. 2 is a schematic cross-sectional view of a far-infrared ray irradiating device which is a part of the mineral-containing water (A) producing device included in the mineral-functional water producing facility shown in FIG. It is a block diagram of the mineral containing water (B) manufacturing apparatus which comprises the mineral functional water manufacturing equipment shown in FIG. It is a front view which shows the mineral containing water (B) manufacturing apparatus which comprises the mineral functional water manufacturing equipment shown in FIG. FIG. 9 is a side view of the apparatus for producing mineral-containing water (B) shown in FIG. 8. FIG. 9 is a partially omitted perspective view showing the configuration of the apparatus for producing mineral-containing water (B) shown in FIG. 8. It is a side view of the water flow container which comprises the mineral containing water (B) manufacturing apparatus shown in FIG. The spectral emissivity spectrum of a sample in which 20 parts by weight of the mineral functional water of Example 1 was immobilized on 100 parts by weight of a ceramic carrier, and the spectral emissivity spectrum (theoretical value) of a black body (measurement temperature: 25 ° C., Wavelength range: 4 to 24 μm, carrier: ceramic powder). It is a figure which shows the radiation ratio (measurement temperature: 25 degreeC) of the black body of the sample which fixed 20 weight part of mineral functional waters of Example 1 with respect to 100 weight part of ceramic carriers. The spectral emissivity spectrum of a sample in which 20 parts by weight of the mineral functional water of Example 2 was immobilized with respect to 100 parts by weight of a ceramic carrier, and the spectral emissivity spectrum (theoretical value) of a black body (measurement temperature: 25 ° C., Wavelength range: 4 to 24 μm, carrier: ceramic powder). It is a figure which shows the change of mouse | mouth recombinant PrP amount before and after the mineral water treatment (Direct ELISA method). It is a figure which shows the change of the scrapie-infected hamster abnormal prion amount (Direct ELISA method). It is a western blotting image which shows the result of having evaluated the effect with respect to abnormal prion protein of mineral water treatment (6 microliter / lane). It is a western blotting image which shows the result of having evaluated the effect with respect to abnormal prion protein of mineral water treatment (3 microliter / lane). It is an immunostaining image of a brain tissue section of hamster number P-1 (positive control). It is an immunostaining image of a brain tissue section of hamster number S-1. It is an immunostaining image of a brain tissue section of hamster number S-3.

DESCRIPTION OF SYMBOLS 1 Mineral functional water production equipment 2 Mineral-containing water (A) production device 3 Mineral-containing water (B) production device 10 Raw material aqueous solution production means 11, W water 12 Mineral imparting material (A)
DESCRIPTION OF SYMBOLS 13 Reaction container 13a Wall 14 Insulator 15 Conductive wire 16 Ultrasonic wave generating means 17 DC power supply 18a, 18b, 18c Circulation path 19 Drain 20, 20 Opening control valve 21, 25 Drain valve 22 Storage tank 24 Drain pipe 26 Water temperature gauge 29, 29a to 29g, 29s, 29t Conductive cable 30 Terminal 31 Storage container 31f Hook 40 Processing container 41 Raw material mineral aqueous solution (A)
42 stirring blades 43 far-infrared ray generating means 44 mineral-containing water (A)
45 Mineral-containing water (B)
46 Mixing tank 47 Mineral functional water 51 First water container 52 Second water container 53 Third water container 54 Fourth water container 55 Fifth water container 56 Sixth water container 51a-56a Main body 51b- 56b Switch button 51c-56c Axis 51d-56d Lid 51f-56f Flange 51m-56m Mineral material (B)
51p-56p Detour water path 51v-56v Water flow switching valve 57, 57x, 57y Water supply path 57a Inlet port 57b Outlet port 57c Mesh strainer 57d Automatic air valve 58 Operation panel 59 Signal cable 60 Frame 61 Caster 62 Level adjuster 63 Raw water tank DC DC current DW tap water R water flow

  Hereinafter, the present invention will be described in detail with reference to examples and the like. However, the present invention is not limited to the following examples and the like, and can be arbitrarily modified and implemented without departing from the gist of the present invention. In this specification, “to” is used as an expression including numerical values or physical quantities before and after “to”.

<1. Method for reducing infectivity of abnormal prion protein>
The present invention relates to a method for reducing the infectivity of an abnormal prion protein, which reduces the infectivity of an abnormal prion protein by contacting the abnormal prion protein with a mineral water containing a mineral component that emits electromagnetic waves.
Further, the present invention relates to an abnormal prion protein which suppresses the growth of an abnormal prion protein by contacting the normal prion protein with a mineral functional water containing a mineral component that emits electromagnetic waves to decompose the normal prion protein. The present invention relates to a method for reducing infectivity of a protein.
These may be collectively referred to as “the method for reducing infectivity of the present invention” or “the method of the present invention”. Further, the “mineral functional water containing an electromagnetic wave-radiating mineral component” according to the method for reducing infectivity of the present invention is hereinafter referred to as “mineral functional water of the present invention”.

  In the method for reducing infectivity of the present invention, the amount of abnormal prion protein is brought into contact with the abnormal functional prion protein by directly or indirectly contacting the mineral functional water of the present invention with the abnormal functional prion protein. And reduce the infectivity of the abnormal prion protein.

  The amount of the prion protein can be determined by, for example, a direct ELISA method or a Western blocking method described in Examples. At this stage, the mechanism of the infectiousness-reducing action is not clear at present, but one of the actions is the action derived from the electromagnetic waves emitted by the mineral components contained in the mineral-functional water. As the electromagnetic wave, a terahertz wave, which is a wavelength that induces intermolecular motion in a protein, is effective. Therefore, it is preferable that the mineral component in the mineral water is a mineral component that emits electromagnetic waves including terahertz waves.

A protein formed by the binding of amino acids exhibits a normal function due to its tertiary structure. However, in the process of starting from the primary structure, obtaining the tertiary tertiary formation and taking the quaternary folding structure, some chemical physics takes place. It is thought that the connective tissue is degenerated on each structure due to an external influence or a virus or other internal influence, which leads to a change in a lesion of a living tissue.
Since individual amino acids have been confirmed to have unique wavelength absorption in the terahertz wave region, it is presumed that proteins that are a collection of amino acids also have terahertz wave absorption unique to each protein. Therefore, it is possible to regenerate the three-dimensional structure of the protein by radiation having a wavelength (for example, a wavelength of about 300 μm) that is absorbed by the biopolymer at the protein level.

  In the method of the present invention, the abnormal prion protein is decomposed by the action derived from the electromagnetic wave emitted by the mineral component contained in the mineral-functional water, or the abnormal prion protein is restored to a normal structure by restoring the abnormal prion protein to a normal structure. The amount has decreased. As the electromagnetic wave, a terahertz wave, which is a wavelength that induces intermolecular motion in a protein, is effective.

  Although the mechanism of the mineral functional water of the present invention is not completely clear, contacting the mineral functional water of the present invention with the normal prion protein at a high temperature (for example, 60 ° C.) converts the normal prion protein into a normal prion protein. May have the effect of decomposing. Therefore, the normal prion protein is brought into contact with a mineral functional water containing a mineral component that emits electromagnetic waves at a high temperature (for example, 60 ° C.) to decompose the normal prion protein, thereby increasing the growth of the abnormal prion protein. There is a possibility that it can be suppressed.

<2. Mineral functional water>
The mineral water used in the method for reducing infectivity according to the present invention contains a mineral component that emits electromagnetic waves. In addition, about the raw material of mineral-functional water of this invention, and production conditions, <3. Method for Producing Mineral Functional Water of the Present Invention>.

  In the present specification, “mineral functional water” means a water that contains a mineral component and exhibits at least one or more effective effects. Further, in the present specification, “mineral-containing water” is a raw material water in a previous stage when producing mineral functional water, and the mineral-containing water also contains a mineral component. The details will be described later as the method for producing mineral functional water of the present invention. In addition, the mineral-containing water may or may not have an effective effect by itself.

In this specification, the term “mineral component” does not mean “inorganic component (including trace elements) excluding four elements (carbon, hydrogen, nitrogen, and oxygen)” which is a definition of mineral in a narrow sense. If it coexists with an inorganic component, it may contain the four elements (carbon / hydrogen / nitrogen / oxygen) excluded in a narrow definition. Therefore, for example, the “plant-derived mineral component” is a concept including a case where a plant-derived organic component is included together with a plant-derived inorganic component such as calcium.
Examples of inorganic components (constituting mineral components) include sodium, potassium, calcium, magnesium, and phosphorus, and trace elements such as iron, zinc, copper, manganese, iodine, selenium, chromium, and molybdenum. It can be exemplified, but not limited to this.

  The mineral functional water of the present invention is not limited as long as it contains a mineral component that emits electromagnetic waves and reduces the infectivity of abnormal prion proteins. The method for producing mineral functional water of the present invention> is used using the production apparatus and the raw materials described in the above.

One preferred embodiment of the mineral functional water of the present invention has, for example, a pH of 12 or more. When the mineral functional water of the present invention has a pH of 12 or more, the peptide bond and the SS bond are easily cleaved by the high reducing action caused by the strong alkali, the absolute amount of the abnormal prion protein is reduced, and the infectivity is reduced. It tends to decrease.
The pH is a value obtained by digitizing the pH of the mineral water measured with a pH meter. The pH meter is not limited to those shown in the examples.

In particular, a preferred example of the mineral functional water of the present invention is a mineral functional water CAC-717 manufactured by Riken Technosystem Co., Ltd.
The mineral functional water CAC-717 is a mineral functional water having a pH of 12 or more and containing a complex of calcium and carbon as a mineral component that emits electromagnetic waves. Mineral functional water CAC-717 has a small pH fluctuation and can maintain an alkaline state. The reason why the mineral-functional water CAC-717 can maintain an alkaline state with little pH fluctuation is not completely clear at this stage, but a complex of calcium and carbon derived from plant and woody plants as raw materials is not clear. It has a function as a pH buffer, and may suppress pH fluctuation.

  Another preferred example of the mineral functional water of the present invention is a mineral functional water A20ACA-717 manufactured by Riken Techno System Co., Ltd., which corresponds to Example 2 described later. Mineral functional water A20ACA-717 is a mineral functional water containing a complex of calcium and carbon as a mineral component that emits electromagnetic waves.

  One preferred embodiment of the mineral functional water of the present invention is that the spectral emissivity spectrum of the mineral functional water of the present invention satisfies the following requirement (i). Since it is difficult to directly measure the spectral emissivity of the liquid sample, a method of measuring the spectral emissivity by fixing the spectral emissivity to a reference carrier is usually employed. The spectral emissivity spectrum of the mineral water of the present invention is measured by immobilizing the mineral water on a ceramic powder for carrying. For example, mineral functional water CAC-717 is one of the mineral functional waters that meets this requirement.

Requirement (i):
(I) A black body at a wavelength of 5 to 7 μm and a wavelength of 14 to 24 μm in a sample in which 15 parts by weight or more (preferably 20 parts by weight or more) of the mineral functional water is immobilized on 100 parts by weight of the ceramic carrier. Average radiation ratio (measuring temperature: 25 ° C.) with respect to 90% or more.

That is, the mineral functional water of the present invention sums the values between the wavelengths of 5 to 7 μm and the wavelengths of 14 to 24 μm in the radiation ratio profile for the black body at 25 ° C., and averages the values (for the black body at 25 ° C.). A) When the average emission ratio is between 5 and 7 μm and between 14 and 24 μm, the average emission ratio is preferably 90% or more.
Radiation at a wavelength of 5 to 7 μm and a wavelength of 14 to 24 μm with respect to a black body at 25 ° C. corresponds to the mid-infrared ray. Has the property of reaching.
That is, the mineral-functional water of the present invention may be exhibiting a beneficial effect by this mid-infrared ray.

  Specifically, a preferred embodiment of the mineral functional water is a spectral emissivity in a wavelength range of 4 μm to 24 μm in a sample in which 20 parts by weight of the mineral functional water is immobilized on 100 parts by weight of the ceramic carrier. The spectrum (measuring temperature: 25 ° C.) shows a specific shape (the shape shown in FIG. 12 or FIG. 14) as shown in the examples. Details will be described later in Examples.

  In the present specification, the “emissivity” is a ratio (JIS Z 8117 (2002)) between the radiant emittance of a radiator and the radiant emittance of a black body having the same temperature as the radiator, and “spectral emissivity” "Indicates the ratio of the radiation of the sample when the emissivity of the black body at that temperature is 100%. The sample to be evaluated has a unique spectral emissivity spectrum. The “black body” refers to an object that absorbs incident light at 100% and has the maximum energy radiation ability, and none of them theoretically exhibits a radiation ability greater than a black body.

  The method for measuring the spectral emissivity spectrum is specified in JIS R 1801 (2002), and has an apparatus configuration in accordance with JIS R 1801 (2002), and emissivity measurement using Fourier transform infrared spectrophotometry (FTIR). It can be measured by the system. As a preferred example of the emissivity measuring system, a far-infrared emissivity measuring device (JIR-E500) manufactured by JEOL Ltd. can be mentioned.

  Since it is difficult to directly measure the spectral emissivity of the liquid sample, a method of measuring the spectral emissivity by fixing the spectral emissivity to a reference carrier is usually employed. The spectral emissivity spectrum of the mineral water of the present invention is measured by immobilizing the mineral water on a ceramic powder for carrying. Details will be described later in Examples.

  Although there are many points that are not clear about various actions of the mineral functional water of the present invention, an estimation mechanism will be described.

  First, the mineral component contained in the mineral-functional water of the present invention may form a special structure. As indirect evidence, a meso-scale structure (hereinafter referred to as a “meso-structure”) is formed by evaluation with an electron microscope obtained by drying and depositing the mineral water of the present invention. The mineral components after drying are aggregated crystalline substances.

As described above, in a preferred embodiment of the mineral functional water of the present invention, a strongly alkaline state of pH 12 or more can be maintained. This may be due to the direct discharge action of the mesostructured fine particles of minerals dispersed in water on water. At pH 12, the binding of proteins that form the cell membrane of unicellular organisms or viruses due to their hydrolytic properties (peptide binding) It is possible that the electromagnetic wave emitted by the mineral component acts on this to act synergistically to reduce the infectivity of the abnormal prion protein.
That is, the mineral component contained in the mineral functional water of the present invention is highly likely to contain at least a part of the mineral component as mesostructured fine particles. Although the details are not completely clear at this stage, since the mineral component is not a completely water-soluble component and is dispersed in the functional water as insoluble fine particles (meso-structured fine particles), the functional water of the present invention is It is presumed to exhibit the action having.

Note that the mesostructured fine particles, which are aggregated crystalline materials, are fine particles having a particle size of about 50 to 500 nm, have a self-power of negative potential in the structure based on the free electron trapping property, and further have a hydrogen storage effect and a terahertz electromagnetic wave. It is presumed to have the ability to generate. The meso-structured fine particles can continuously generate a high voltage with a pulse, discharge the surrounding water molecules and break down the water molecules into H ⁺ ions and OH ^- ions by electrolysis. Because of the negative electric potential and the physical properties of the hydrogen storage effect, the H ⁺ ions are given electrons from the mesostructured fine particles and returned to hydrogen atoms (H), and then accumulated and immobilized inside the mesostructured fine particles. This leads to a relative decrease in H ⁺ ions, which is presumed to be in a strong alkali state with a pH of 12 or more, for example.

  The above-mentioned estimation mechanism is merely an estimation at the present time, and even if a mechanism different from the above is discovered in the future, useful effects in the mineral-functional water of the present invention are interpreted in a limited manner. It should not be. In addition, the mineral functional water of the present invention may have a plurality of different useful effects, and the expression mechanism may be different for each of the effects.

  The mineral functional water of the present invention may be diluted with an appropriate diluting solvent (such as water or alcohol) as long as the object of the present invention is not impaired.

  The mineral functional water of the present invention may contain any component as long as its effectiveness is not impaired. The optional components are not particularly limited as long as they do not impair the purpose of the present invention, and include, for example, known suspending agents and emulsions. The mixing ratio is arbitrary as long as the object of the present invention is not impaired.

(Use of the mineral functional water of the present invention)
Since the mineral functional water of the present invention has an infectivity reducing effect on abnormal prion protein, the mineral functional water of the present invention is directly or indirectly contacted with a target containing an abnormal prion protein. Thereby, the abnormal prion protein can be inactivated and its infectivity can be reduced.
As described above, according to the method of the present invention, prion disease derived from abnormal prion protein in humans and animals can be prevented, and further improvement of prion disease can be expected.

  Representative prion diseases of interest include Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler-Scheinker syndrome (GSS) and fatal familial insomnia (FFI) in humans, and non-human. Examples include bovine spongiform encephalopathy (BSE), scrapie, deer chronic wasting disease (CWD), and transmissible mink encephalopathy (TME).

In addition, prevention and improvement of prion disease can be expected by taking the mineral-functional water of the present invention into humans or animals suffering from prion disease.
That is, the pharmaceutical composition for prevention and treatment of prion disease of the present invention (hereinafter sometimes referred to as “the pharmaceutical composition of the present invention”) contains, as an active ingredient, mineral functional water containing a mineral component that emits electromagnetic waves. It is characterized by doing. The mineral component is preferably a mineral component derived from mineral functional water CAC-717 manufactured by Riken Technosystem Co., Ltd.

The pharmaceutical composition of the present invention can be used as a quasi-drug or pharmaceutical, and an effective amount thereof is blended with a pharmaceutically acceptable carrier, and is orally or parenterally administered as a solid preparation or a liquid preparation. be able to. The dosage form may be any dosage form used for normal oral or parenteral administration.
As the dosage form used for oral administration or parenteral administration, specific examples include solid preparations such as powders, granules, tablets, capsules, and troches. Examples of liquid preparations include liquid preparations for internal use, liquid preparations for external use, suspensions, emulsions, syrups, injections, and infusions. These dosage forms and other dosage forms are appropriately selected depending on the purpose. These preparations can be prepared by conventional methods for preparation.

  In addition, the pharmaceutical composition of the present invention is not particularly limited as long as the mineral functional water as an active ingredient is contained in a sufficient amount to exhibit an infectivity reducing effect on abnormal prion protein. It can take any form or type. Therefore, in addition to quasi-drugs or pharmaceuticals, they can be used, for example, as functional foods, animal feeds, and the like.

<3. Method for producing mineral functional water>
Although the production method of the mineral functional water of the present invention is not particularly limited, it is preferable to use the apparatus disclosed in Patent Document 2 (Japanese Patent Application Laid-Open No. 2011-56366) to obtain the method disclosed in this document. It can be manufactured by a method according to.
In addition to the production method using this production apparatus, the production method is not limited as long as a mineral functional water containing a mineral component having an electromagnetic wave radiation action can be obtained.

  Hereinafter, a preferred embodiment of the method for producing mineral functional water of the present invention using the apparatus disclosed in Patent Document 2 (Japanese Patent Application Laid-Open No. 2011-56366) will be described with reference to the drawings. The following description corresponds to a method for producing a mineral functional water CAC-717 manufactured by Riken Techno System Co., Ltd., which is a suitable mineral functional water. Other mineral functional waters can be produced by appropriate changes.

  As shown in FIG. 1, the mineral-functional water producing equipment 1 was produced by a mineral-containing water (A) producing device 2, a mineral-containing water (B) producing device 3, and a mineral-containing water (A) producing device 2. A mixing tank 46 as mixing means for mixing the mineral-containing water (B) 45 produced by the mineral-containing water (B) producing apparatus 3 with the mineral-containing water (B) 45 to form a mineral-functional water 47; ing.

  The mineral-containing water (A) production apparatus 2 produces a raw mineral aqueous solution (A) 41 by using water 11 supplied from a tap water and a mineral imparting material (A) 12 (see FIG. 4) as a raw material. And a far-infrared ray generating means 43 for irradiating the raw mineral aqueous solution (A) 41 obtained by the raw mineral aqueous solution producing means 10 with far-infrared rays to change it into mineral-containing water (A) 44.

  The mineral-containing water (B) producing device 3 forms the mineral-containing water (B) 45 containing the mineral component eluted from the mineral-imparting material by passing the water W supplied from the outside through the water containers 51 to 56. Has the function of

  Hereinafter, the mineral-containing water (A) producing device 2 and the mineral-containing water (B) producing device 3 will be described in detail.

(3-1: Mineral-containing water (A) production apparatus)
Next, the mineral-containing water (A) producing apparatus 2 constituting the mineral-functional water producing facility 1 shown in FIG. 1 will be described with reference to FIGS. As shown in FIG. 1, a mineral-containing water (A) producing apparatus 2 uses a raw mineral water solution (A) 41 using water 11 supplied from a tap water and a mineral-imparting material (A) 12 (see FIG. 4) as a raw material. And a mineral-containing water (A) 44 by irradiating far-infrared rays to the mineral-containing water (A) solution 41 obtained by the raw-mineral-water solution producing means 10 (see FIG. 2). Far infrared ray generating means 43 (see FIG. 6).

  As shown in FIGS. 2 and 3, the raw material mineral aqueous solution producing means 10 includes a reaction vessel 13 capable of storing water 11 and a mineral-imparting material (A) 12, and a reaction vessel 13 covered with an insulator 14. A conductive wire 15 immersed in water 11, ultrasonic wave generating means 16 for applying ultrasonic vibration to the water 11 in the reaction vessel 13, and a DC power supply device for conducting DC current DC to the conductive wire 15. 17, and circulation paths 18 a and 18 b and a circulation pump P which are means for generating a water flow R in the same direction as the direct current DC in the water 11 around the conductive wire 15. The DC power supply 17, the ultrasonic generator 16, and the circulation pump P all operate by power supply from a general commercial power supply.

  The reaction vessel 13 is in the shape of an inverted conical cylinder with an open upper surface, and a drain port 19 is provided at the bottom corresponding to the apex thereof. The drain port 19 has a circulation path 18a communicating with the suction port P1 of the circulation pump P. An opening control valve 20 for adjusting the amount of drainage to the circulation path 18a and a drainage valve 21 for discharging water and the like in the reaction vessel 13 are provided immediately below the drainage port 19.

  The base end of the circulation path 18b is connected to the discharge port P2 of the circulation pump P, and the tip end of the circulation path 18b is connected to the storage tank 22. A base end of a circulation path 18c for feeding the water 11 in the storage tank 22 into the reaction vessel 13 is connected near the bottom of the outer periphery of the storage tank 22, and a tip end of the circulation path 18c is an opening of the reaction vessel 13. It is plumbed at a position facing. The circulation path 18c is provided with an opening adjustment valve 23 for adjusting the amount of water sent from the storage tank 22 to the reaction vessel 13.

  A drain pipe 24 having a drain valve 25 and a water temperature gauge 26 is connected to the bottom of the storage tank 22 in a hanging manner. When the drain valve 25 is opened as necessary, the water in the storage tank 22 can be discharged from the lower end of the drain pipe 24, and at this time, the temperature of the water 11 passing through the drain pipe 24 is measured by the water temperature meter 26. be able to.

  As shown in FIG. 5, a plurality of conductive cables 29 (29 a to 29 g) including a conductive wire 15 and an insulator 14 covering the conductive wire 15 are formed in a ring shape at a plurality of positions at different depths in the reaction vessel 13. These annular conductive cables 29 a to 29 g are arranged substantially coaxially with the reaction vessel 13. The inner diameter of each of the conductive cables 29a to 29g is reduced stepwise in accordance with the inner diameter of the inverted conical cylindrical reaction vessel 13, and has an inner diameter corresponding to each location. Since each of the conductive cables 29a to 29g is detachably connected to an insulative terminal 30 provided on the wall 13a of the reaction vessel 13, an annular portion can be removed from the terminal 30 as necessary. Can be attached.

  At a portion corresponding to the axis in the reaction vessel 13, a bottomed cylindrical storage vessel 31 formed of an insulating mesh is disposed, and the storage vessel 31 is filled with the mineral-imparting material (A) 12. Have been. The storage container 31 is detachably locked to the upper edge of the wall 13a of the reaction container 13 by a hook 31f provided at an upper portion thereof.

  As shown in FIG. 2, conductive cables 29s and 29t are spirally wound around the outer circumferences of the circulation paths 18a and 18b, respectively, and a DC current DC is supplied from the DC power supply 17 to these conductive cables 29s and 29t. You. The direction of the direct current DC flowing through the conductive cables 29s, 29t is set to substantially match the direction of the water flow flowing in the circulation paths 18a, 18b.

  In the raw mineral aqueous solution production means 10, a predetermined amount of water 11 was put in the reaction vessel 13 and the storage tank 22, and the storage vessel 31 filled with the mineral-imparting material (A) 12 was set at the center of the reaction vessel 13. Then, while operating the circulation pump P, the opening control valve 20 at the bottom of the reaction vessel 13 and the opening control valve 23 of the circulation path 18c are adjusted, and the drain port 19, the circulation path 18a, the circulation pump P The water 11 is circulated so as to return to the upper portion of the reaction vessel 13 again via the circulation path 18b, the storage tank 22, and the circulation path 18c. Then, when the DC power supply device 17 and the ultrasonic wave generating means 16 are operated, the elution reaction of the mineral component from the mineral providing material (A) 12 in the storage container 31 to the water 11 starts.

The working conditions for producing the raw mineral aqueous solution (A) using the raw mineral aqueous solution producing means 10 are not particularly limited. In the present embodiment, the production of the raw mineral aqueous solution (A) is performed under the following working conditions. .
(1) A direct current DC having a voltage of 8000 to 8600 V and a current of 0.05 to 0.1 A was conducted to the conductive cables 29, 29s, and 29t. Note that the insulator 14 constituting the conductive cable 29 and the like is formed of polytetrafluoroethylene resin.
(2) The mineral-imparting material (A) 12 filled in the reaction vessel 13 is filled with 10 to 15% by mass of water 11. The specific description of the mineral imparting material (A) 12 will be described later.
(3) The water 11 only needs to contain an electrolyte so that the direct current DC acts. For example, a solution in which about 10 g of sodium carbonate as an electrolyte is dissolved in 100 liters of water is used. However, groundwater can be used as it is.
(4) The ultrasonic wave generating means 16 generates an ultrasonic wave having a frequency of 30 to 100 kHz so that the ultrasonic vibration part (not shown) directly touches the water 11 in the reaction vessel 13 and vibrates. Ultrasonic wave generating means 16 is arranged.

  When the raw mineral aqueous solution production means 10 is operated under such conditions, a water flow R is generated in the reaction vessel 13 while being rotated in the left-handed screw direction and drawn into the drain port 19, and the water discharged from the drain port 19 is discharged. No. 11, the state of returning to the inside of the reaction vessel 13 again via the circulation paths 18a and 18b described above is continued.

  Therefore, the mineral component from the mineral-giving material (A) 12 is quickly mixed in the water 11 by the stirring action by the water flow R, the action of the direct current flowing through the conductive cable 29, and the ultrasonic vibration applied to the water 11 by the ultrasonic generating means 16. And the raw material mineral aqueous solution (A) in which the necessary mineral components are appropriately dissolved can be efficiently produced.

  In the raw mineral aqueous solution producing means 10, a plurality of annular conductive cables 29a to 29g are wired substantially coaxially in the reaction vessel 13 and a water flow R which rotates in the left-handed screw direction in the reaction vessel 13 is generated. ing. Accordingly, a relatively dense electric energy field can be formed in the reaction vessel 13 having a constant volume, and the raw material mineral aqueous solution (A) can be efficiently produced in the reaction vessel 13 having a relatively small volume. .

  Further, since the reaction vessel 13 is in the shape of an inverted conical cylinder, the water flow R flowing along the plurality of annular conductive cables 29a to 29g can be generated relatively easily and stably. Is promoted. In addition, since the flow rate of the water flow R flowing in the inverted conical cylindrical reaction vessel 13 increases toward the drain port 19 at the bottom of the reaction vessel 13, the frequency of contact with the mineral-imparting material (A) 12 also increases, It is possible to increase the amount of minerals that capture and ionize the free electrons e present in the water 11.

  Furthermore, since the storage tank 22 for storing and discharging the water 11 is provided between the circulation paths 18b and 18c, the mineral elution reaction can be advanced while circulating the water 11 in an amount exceeding the volume of the reaction vessel 13. It is possible. Therefore, the raw material mineral aqueous solution (A) can be efficiently mass-produced.

  When the circulation pump P is continuously operated to continue these reactions, a raw mineral aqueous solution (A) in which a mineral component is eluted is finally produced. Depending on the size of the drain port 19 at the bottom of the reaction vessel 13 and the amount of circulating water, the shape of the reaction vessel 13 (particularly, the angle γ between the axis C and the wall 13a shown in FIG. The appearance state of the electron e can be controlled, and the action of the free electron e on the mineral-giving material (A) 12 affects the water solubility of the mineral component.

  When the raw material mineral aqueous solution (A) is formed, the raw material mineral aqueous solution (A) 41 is transferred into the processing vessel 40 shown in FIG. In this case, the residue of the mineral imparting material (A) 12 leaked from the storage container 31 in the reaction container 13 can be discharged from the drain valve 21 at the bottom of the reaction container 13. The raw material mineral aqueous solution (A) 41 accommodated in the processing container 40 is irradiated with far-infrared rays by the far-infrared ray generating means 43 arranged inside the processing container 40 while being slowly stirred by the stirring blades 42.

  The far-infrared ray generating means 43 only needs to generate far-infrared rays having a wavelength of about 6 to 14 μm. The material and the generating means are not limited. However, at 25 ° C., those having an emission ratio of 85% or more to blackbody radiation in the 6 to 14 μm wavelength range are desirable.

  In the raw material mineral aqueous solution production means 10 shown in FIG. 2, the mineral component contained in the mineral-giving material (A) 12 is quickly reduced by the stirring action of the water flow R, the action of the direct current DC flowing through the conductive wire 15, and the ultrasonic vibration. The mineral components eluted into the water 11 and the necessary mineral components are appropriately dissolved therein, so that the mineral aqueous solution 41 can be efficiently produced.

  Then, in the far-infrared ray generating means 43 shown in FIG. 6, by irradiating the mineral aqueous solution 41 with far-infrared rays, the dissolved mineral components and water molecules are fused to increase the electronegativity of the mineral-containing water (A) 44. Is formed.

  In the mineral-containing water (A) producing apparatus 2, the mineral-containing water (A) 44 formed by the above-described process is sent to a mixing tank 46 via a water supply path 57y as shown in FIG. In 46, it is mixed with the mineral-containing water (B) 45 sent from the mineral-containing water (B) producing device 3.

Hereinafter, the mineral imparting material (A) will be described.
The mineral-imparting material (A) is a herbaceous plant material consisting of asteraceous plants and a Rosaceae plant, and a woody plant material consisting of one or more woody plants selected from maple, birch, pine and cedar. It contains. As a site to be used, a site where a mineral component is easily eluted, such as a leaf portion, a stem portion, a flower portion, and a bark portion, is appropriately selected, and may be used as it is, or may be used as a dried product.
In addition, other plant plants may be included in addition to plant plants other than Asteraceae and Rosaceae, but it is preferable that only plant plants of the Asteraceae and Rosaceae are included. For example, for unknown reasons, the addition of cruciferous or pinaceous plant plants greatly reduces the control effect of single-celled organisms, one of the useful effects of the mineral-functional water of the present invention.

Examples of the mineral-imparting material (A) include a mineral-imparting material (A ′). The mineral-imparting material (A ′) is, as the plant material, wild thistle (leaves, stems and flowers): 8 to 12% by weight, mugwort (leaves and stems), camellia (leaves and stems). ) Were mixed at a ratio of 8 to 12% by weight, 55 to 65% by weight, and 27 to 33% by weight, dried, and then pulverized, and then dried and pulverized.
17-23% by weight, 8-12% by weight, 65-75% by weight of wild rose (leaf, flower), radish (leaf and stem), and raspberry (leaf, stem and flower), respectively Using a dry and crushed Rosaceae plant that was mixed and dried at a ratio of
A plant material (A1) obtained by mixing the dry and crushed Asteraceous plant and the dry and crushed Rosaceae plant in a ratio of 1: 0.8 to 1: 1.2 (weight ratio);
As the woody plant raw materials, maple (leaves and stems), birch (leaves, stems, and bark), cedars (leaves, stems, and bark) are each 22 to 28% by weight, A woody plant raw material (A2) comprising a dry and crushed product obtained by mixing at a ratio of 22 to 28% by weight and 45 to 55% by weight, drying, and then crushing the mixture.
It is a mineral-imparting material obtained by mixing plant and plant material (A1) and woody plant material (A2) in a weight ratio of 1: 2.7 to 1: 3.3.

Among the mineral-imparting materials (A '), especially as the plant material, wild thistle (leaves, stems and flowers), mugwort (leaves and stems), camellia (leaves and stems), A dried and crushed Asteraceous plant, which was mixed at a ratio of 10% by weight, 60% by weight, and 30% by weight, dried, and crushed, as well as wild rose (leaves and flowers) and radish (leaves and leaves) Stems) and raspberries (leaves, stems and flowers) were mixed at a ratio of 20% by weight, 10% by weight, and 70% by weight, respectively, dried, and crushed. A plant material (A1) obtained by mixing at a ratio of 1: 1 (weight ratio);
As the woody plant raw materials, maple (leaves and stems), birch (leaves, stems and bark), and cedars (leaves, stems and bark) were 25% by weight and 25% by weight, respectively. %, A weight ratio of a woody plant raw material (A2) composed of a dry and crushed product obtained by mixing, drying and then pulverizing the plant material (A1) and the woody plant material (A2). It is preferable to use a mineral-giving material obtained by mixing so that the ratio becomes 1: 3.
As such a plant material (A1), “P-100 (product number)” manufactured by Riken Technosystem Co., Ltd. As a woody plant material (A2), “P-200 (product number)” manufactured by RIKEN Technosystem Co., Ltd. It can be suitably used.

(3-2: Mineral-containing water (B) production apparatus)
Next, the structure, function, and the like of the mineral-containing water (B) producing apparatus 3 will be described with reference to FIGS.
As shown in FIGS. 1 and 7, the mineral-containing water (B) producing apparatus 3 includes first to sixth water containers 51 to 56 filled with different types of mineral imparting materials (B). A water supply path 57 that connects the first water supply vessel 51 to the sixth water supply vessel 56 in series, and a detour connected to the water supply path 57 in a state where the first water supply vessel 51 to the sixth water supply vessel 56 are respectively arranged in parallel. Water passages 51p to 56p, and water flow switching valves 51v to 56v respectively provided at branch portions of the bypass water passages 51p to 56p and the water supply passage 57 are provided.

  The switching operation of the water flow switching valves 51v to 56v is performed by operating six switching buttons 51b to 56b provided on an operation panel 58 connected to the water flow switching valves 51v to 56v by a signal cable 59. Can be. Since the six switching buttons 51b to 56b and the six water flow switching valves 51v to 56v correspond to the respective numbers, if any of the switching buttons 51b to 56b is operated, the water flow of the corresponding number is operated. The switching valves 51v to 56v are switched to change the water flow direction.

Here, the mineral-imparting materials (B) 51 m to 56 m can be preferably produced by mixing raw materials based on limestone, fossil corals, and shells.
First, the components contained in limestone, fossil corals, and shells are analyzed, and the amounts of silicon dioxide, iron oxide, activated carbon, titanium nitride, calcium carbonate, magnesium carbonate, and calcium phosphate are evaluated respectively. Then, limestone, fossil coral, and shells are mixed based on the content of each component to produce a mineral-imparting material (B) 51 m to 56 m.
In addition, it is desirable to control the components contained in the mineral-imparting materials (B) 51 m to 56 m depending on the mixing ratio of limestone, fossil coral, and shells. However, limestone, fossil coral, and shells as raw materials are contained depending on the production area. If necessary, silicon dioxide, iron oxide, activated carbon, titanium nitride, calcium carbonate, magnesium carbonate, and calcium phosphate may be added. In particular, activated carbon is rarely contained in limestone, fossil corals and shells, and is usually added separately.

As the mineral imparting material (B) 51 m to 56 m,
A mixture containing 70% by weight, 15% by weight, and 15% by weight of limestone, fossil coral, and shells, respectively,
A mixture in which the mineral-imparting material (B2) in the second water passage container 52 contains limestone, fossil coral, shells, and activated carbon, respectively, at 40% by weight, 15% by weight, 40% by weight, and 5% by weight;
A mixture in which the mineral-imparting material (B3) in the third water container 53 includes limestone, fossil coral, and shells each containing 80% by weight, 15% by weight, and 5% by weight;
A mixture containing 90% by weight, 5% by weight, and 5% by weight of limestone, fossil coral, and shells, respectively, in the mineral-imparting material (B4) in the fourth water passage container 54;
A mixture in which the mineral-imparting material (B5) in the fifth water passage container 55 includes limestone, fossil coral, and shells each containing 80% by weight, 10% by weight, and 10% by weight;
When the mineral-imparting material (B6) in the sixth water passage container 56 is a mixture containing limestone, fossil coral, and shells at 60% by weight, 30% by weight, and 10% by weight, it is mixed with the mineral-containing water (A). It is possible to obtain mineral-containing water (B) which exhibits an excellent controlling action when the water is applied.

  In particular, the limestone, fossil coral, and shell used in the mineral-imparting materials (B1) to (B6) are preferably the following (1-1) to (1-3).

(1-1) Limestone:
A crushed limestone containing a volcanic ore deposit containing the following components: Pebbles about 3 cm Calcium carbonate: 50% by weight or more Iron oxide: 3 to 9% by weight of iron Total of titanium oxide, titanium carbide, and titanium nitride: 0 0.8% by weight or more Magnesium carbonate: 7 to 10% by weight
As such limestone, “CC-200 (product number)” manufactured by Riken Techno System Co., Ltd. can be suitably used.

(1-2) Fossil coral:
The following two types of fossil corals are mixed at a weight ratio of 1: 9, and a granular material crushed to 3 to 5 mm is produced from about 100 meters underground. Fossil corals whose crystal composition has been modified by heavy pressure are produced from land near Okinawa Amami Oshima. Fossil corals (including calcium carbonate, calcium phosphate and other trace elements)
As such a fossil coral, “CC-300 (product number)” manufactured by Riken Techno System Co., Ltd. can be suitably used.

(1-3) Shell:
Granules obtained by mixing abalone, lycopodium, and barnacle with the same weight and pulverizing to 3 to 5 mm As such a shell, "CC-400 (product number)" manufactured by Riken Techno System Co., Ltd. can be suitably used.

(1-4) Activated carbon As the activated carbon, those produced from arbitrary raw materials can be used, and activated carbon produced from coconut husk is preferred. For example, “CC-500 (product number)” manufactured by Riken Technosystem Co., Ltd., which uses Thai coconut shell as a raw material, may be mentioned.

  If the switching buttons 51b to 56b of the operation panel 58 described above are operated to switch the water flow switching valves 51v to 56v to the water container side, the water flowing through the water supply path 57 is located downstream from the operated water flow switching valve. If the water flows into the first water container 51 to the sixth water container 56, and switches the water flow switching valves 51v to 56v to the bypass water channel side, the water flowing through the water supply path 57 is transmitted from the operated water flow switching valve. It flows into the detour waterways 51p-56p on the downstream side. Therefore, by operating any of the switching buttons 51b to 56b to selectively switch the water flow switching valves 51v to 56v, a different mineral imparting material (B) is provided for each of the first to sixth water containers 51 to 56. The mineral-containing water (B) 45 in which the mineral component eluted from 51 m to 56 m is selectively dissolved can be formed.

  Next, the structure, function, and the like of the actual mineral-containing water (B) production device 3 will be described with reference to FIGS. 8 to 10, the above-described bypass water passages 51p to 56p, water flow switching valves 51v to 56v, operation panel 58, and signal cable 59 are omitted.

  As shown in FIGS. 8 and 9, the mineral-containing water (B) producing apparatus 3 includes a substantially cylindrical first water container 51 to a sixth water container 56 mounted on a gantry 60 and a first water container 56. A raw water tank 63 for storing the water W supplied from the water supply is disposed at the uppermost portion of the gantry 60. The water supply path 57 connects the water passage containers 51 to the sixth water passage containers 56 in series. . In the raw water tank 63, an inorganic porous body 64 having a function of adsorbing impurities in the water W is accommodated. A plurality of casters 61 and a level adjuster 62 are provided at the bottom of the gantry 60. The substantially cylindrical first water container 51 to the sixth water container 56 are mounted on a gantry 60 having a rectangular parallelepiped lattice structure with their respective axes 51c to 56c (see FIG. 9) kept in the horizontal direction. I have. The first water container 51 to the sixth water container 56 are detachable from the gantry 60.

  As shown in FIG. 10, the first water-flowing vessel 51 to the sixth water-flowing vessel 56 have the same structure, and have circular circles formed at flanges 51f to 56f provided at both ends of cylindrical body parts 51a to 56a. An airtight structure is formed by attaching the plate-like lids 51d to 56d. A water inlet 57a communicating with the water supply path 57 is provided at a position located at the lowermost part of the main bodies 51a to 56a when the shaft centers 51c to 56c are in a horizontal state, and the lids 51d to 56d farther from the water inlet 57a are provided. A water outlet 57b communicating with the water supply path 57 is provided at the uppermost portion, and a mesh strainer 57c is attached to the water outlet 57b. An automatic air valve 57d for releasing air in the first through sixth water containers 51-56 is attached to a portion immediately above the water outlet 57b on the outer periphery of the main bodies 51a-56a.

  The water supplied from the upstream water supply passage 57 passes through the water inlet 57a, flows into the first through sixth water containers 51-56, and is provided with the mineral-imparting material (B) filled therein. Since each mineral component elutes into water by contacting with 51 m to 56 m, it becomes water containing a mineral component corresponding to each of the mineral imparting materials (B) 51 m to 56 m and becomes a water supply path downstream from the outlet 57 b. Outflow to 57.

  In the mineral-containing water (B) producing apparatus 3 shown in FIGS. 8 to 10, one of the switching buttons 51 b to 56 b of the operation panel 58 shown in FIG. By passing through at least one of the water-flowing containers 51 to the sixth water-flowing container 56, the mineral-imparting materials (B) 51m to 56m filled in the first water-flowing container 51 to the sixth water-flowing container 56, respectively. The mineral-containing water (B) 45 in which the characteristic mineral component contained is selectively dissolved can be formed.

  Further, in the mineral-containing water (B) production apparatus 3, since the first water passage 51 to the sixth water passage 56 are connected in series by the water passage 57, water is continuously supplied to the water passage 57. By flowing, it is possible to mass-produce the mineral-containing water (B) 45 in which the mineral components corresponding to the mineral-imparting materials (B) 51 m to 56 m in the first water container 51 to the sixth water container 56 are dissolved. .

  The mineral-containing water (B) 45 formed in the mineral-containing water (B) producing device 3 is sent into the mixing tank 46 via a water supply path 57x downstream of the sixth water supply container 56, and Inside, the mineral-functional water 47 is formed by being mixed with the mineral-containing water (A) 44 produced by the mineral-containing water (A) producing apparatus 2 shown in FIG.

The mixing ratio of the mineral-containing water (A) and the mineral-containing water (B) is appropriately determined in consideration of the types of the raw materials contained in the mineral-containing water (A) and the mineral-containing water (B) and the concentrations of the components to be eluted. Is the weight ratio of mineral-containing water (A) to mineral-containing water (B) ([mineral-containing water (A)]: [mineral-containing water (B)]) in the range of 1: 5 to 1:20. Yes, preferably in the range of 1: 7 to 1:12, more preferably in the range of 1:10.
When the amount of the mineral-containing water (A) is too small (the amount of the mineral-containing water (B) is too large), and when the amount of the mineral-containing water (A) is too large (the amount of the mineral-containing water (B) is too small), the mineral functional water Of the active ingredient may be diluted and the desired action may be insufficient.

  As described above, the preferred embodiment of the method for producing mineral functional water of the present invention has been described. However, it is sufficient that the mineral functional water of the present invention having the above-described configuration can be produced, and various configurations other than the preferred embodiment are employed. And should be considered non-limiting. In particular, in the embodiments disclosed herein, matters not explicitly disclosed, such as operating conditions and operating conditions, various parameters, dimensions, weight, and volume of components, deviate from the range usually performed by those skilled in the art. Instead, a value that can be easily assumed by a person skilled in the art is adopted.

  Hereinafter, the present invention will be described more specifically with reference to Examples, but the present invention is not limited thereto.

[Example 1]
<1> Production of Mineral Functional Water Using the mineral functional water production apparatus described in the above embodiment of the present invention as the mineral functional water, the mineral function of Example 1 produced by the above-described production method using the following raw materials and method Water was used.
1. Manufacture of Mineral-Containing Water (A) As a raw material of the mineral-imparting material (A), "P-100 (product number)" manufactured by Riken Technosystem Co., Ltd., as a woody plant raw material (A2) "P-200 (product number)" manufactured by Riken Techno System Co., Ltd. was used.
"P-100" is a plant material (A1) obtained by mixing the following dried and crushed asteraceous plants and dried and crushed roseaceous plants at a ratio of 1: 1 (weight ratio), and "P-200" is And woody plant materials (A2) described below.

(A1) Plant material (dried plant material)
(A1-1) Dry and Pulverized Asteraceous Plant Wild thistle (leaves, stems and flowers), mugwort (leaves and stems), and camellia (leaves and stems) were each 10% by weight, 60% by weight. What was mixed at a ratio of 30% by weight, dried, and then pulverized.
(A1-2) Dry and Pulverized Rosaceae Plants Wild rose (leaves and flowers), radish (leaves and stems), and raspberry (leaves, stems and flowers) were each 20% by weight, 10% by weight, What was mixed at a ratio of 70% by weight, dried, and then pulverized.

(A2) Woody plant material (dried woody plant)
Maple (leaves and stems), birch (leaves, stems, and bark), cedars (leaves, stems, and bark) are 25% by weight, 25% by weight, and 50% by weight, respectively. A mixture obtained by mixing, drying, and then pulverizing.

  A mineral-imparting material (A) obtained by mixing the plant material (A1) and the woody plant material (A2) at a ratio of 1: 3 (weight ratio) was used in a mineral-containing water (A) production apparatus 2 shown in FIG. The raw mineral aqueous solution producing means 10 (see FIG. 2) is put into the raw mineral aqueous solution producing means 10 at a concentration of 10 to 15% by weight, and a direct current (8300 V DC, 100 mA) is conducted to the conductive wire of the raw mineral aqueous solution producing means 10. A water flow in the same direction as the direct current was generated in the water around the sample, and ultrasonic vibration (oscillation frequency 50 kHz, amplitude 1.5 / 1000 mm) was applied to the water to form a raw mineral aqueous solution (A). Subsequently, the mineral-containing water (A) of Example 1 was obtained by irradiating the raw mineral aqueous solution (A) supplied to the far-infrared ray generating means 43 at the subsequent stage with far-infrared rays (wavelength: 6 to 14 μm).

2. Production of Mineral-Containing Water (B) As a raw material of the mineral-imparting material (B), a mixture obtained by pulverizing and mixing limestone, fossil coral, shells, and activated carbon was used. The raw materials of the mineral-giving materials (B) and the mixtures (mineral-giving materials (B1) to (B6)) used in the first to sixth water-passing containers are as follows.

(1) Raw material (1-1) Limestone: "CC-200 (part number)" manufactured by Riken Techno System Co., Ltd.
A crushed limestone containing a volcanic ore deposit containing the following components: Pebbles about 3 cm Calcium carbonate: 50% by weight or more Iron oxide: 3 to 9% by weight of iron Total of titanium oxide, titanium carbide, and titanium nitride: 0 0.8% by weight or more Magnesium carbonate: 7 to 10% by weight

(1-2) Fossil coral: "CC-300 (part number)" manufactured by Riken Techno System Co., Ltd.
The following two types of fossil corals are mixed at a weight ratio of 1: 9, granulated to 3 to 5 mm, produced from about 100 meters underground, fossil corals whose crystal composition has been modified by gravity, and from land near Okinawa Amami Oshima Fossil corals produced (including calcium carbonate, calcium phosphate and other trace elements)

(1-3) Shell: "CC-400 (part number)" manufactured by Riken Techno System Co., Ltd.
-Granules obtained by mixing abalone, lycopodium and barnacle with the same weight and grinding to 3 to 5 mm

(1-4) Activated carbon (use only the second water container): “CC-500 (part number)” manufactured by Riken Techno System Co., Ltd.

(2) Percentage of water used in the first to sixth water tanks ・ First water tank:
Mineral-imparting material (B1): a mixture of limestone, fossil coral, and shells each of 70% by weight, 15% by weight, and 15% by weight.
Mineral-imparting material (B2): a mixture of limestone, fossil coral, shell, and activated carbon, each of which is 40% by weight, 15% by weight, 40% by weight, and 5% by weight (corresponding to silicon dioxide and activated carbon)
・ Third water container:
Mineral-imparting material (B3): a mixture of limestone, fossil coral, and shells of 80% by weight, 15% by weight, and 5% by weight, respectively.
Mineral-imparting material (B4): 90% by weight, 5% by weight, 5% by weight of limestone, fossil coral, and shell mixed respectively. Fifth water passage container:
Mineral-imparting material (B5): A mixture of limestone, fossil coral, and shells each of 80% by weight, 10% by weight, and 10% by weight.
Mineral-imparting material (B6): A mixture of limestone, fossil coral, and shells each of 60% by weight, 30% by weight, and 10% by weight.

  In the apparatus 3 for producing mineral-containing water (B) having the configuration shown in FIG. 1, the water is passed through the first to sixth water-flowing containers using the mineral-giving materials (B1) to (B6), whereby the mineral-containing water (B) ) Got. (B1) to (B6) were 50 kg (300 kg in total), the amount of water to be circulated was set to 1000 kg, and the flow rate was set to 500 mL / 40 s.

The mineral-containing water (A) and the mineral-containing water (B) of Example 1 formed by the above method were mixed at a ratio of 1:10 (weight ratio) to obtain a mineral-functional water of Example 1. It was pH 12.5 when the mineral functional water of Example 1 was measured with the pH meter (Toko Chemical Laboratory glass electrode type hydrogen ion concentration indicator TPX-90).
The mineral functional water of Example 1 is equivalent to the mineral functional water CAC-717 (Terra Protect (trade name), CAC-717 (part number), development product number CA-C-01) manufactured by Riken Technosystem Co., Ltd. I do.

<2> Evaluation of spectral emissivity The spectral emissivity of the sample in which the mineral-functional water of Example 1 was immobilized on the ceramic carrier was measured with a far-infrared emissivity measuring device (JIR-E500 manufactured by JEOL Ltd.). did. The apparatus includes a Fourier transform infrared spectrophotometer (FTIR) main body, a black body furnace, a sample heating furnace, a temperature controller, and an attached optical system.
An evaluation sample of the spectral emissivity was prepared in the following procedure.
20 parts by weight of the mineral functional water of Example 1 was added to 100 parts by weight of a ceramic powder for support (rock powder produced by Amakusa Oyanoshima) to obtain a clay state. This was processed into a flat plate having a thickness of about 5 mm and a circular surface with a diameter of 2 cm and baked at 1000 ° C. to obtain an evaluation sample in which the mineral components contained in the sample (mineral functional water) were immobilized. .
FIG. 12 shows a spectral emissivity spectrum (measurement temperature: 25 ° C., wavelength range: 4 to 24 μm) of the mineral functional water of Example 1 which is a measurement sample. FIG. 12 also shows the spectral emissivity spectrum (theoretical value) of the black body. In FIG. 12, the vertical axis indicates the intensity of radiant energy, and is indicated by the number of W per square cm. The curve of “sample” means that the closer to the curve of the black body, the higher the radiation ability.

FIG. 13 shows the emissivity (wavelength range: 4 to 24 μm) obtained from the spectral emissivity spectrum of the measurement sample and the spectral emissivity spectrum (theoretical value) of the black body.
The average radiation ratio between the wavelengths of 5 to 7 μm and between the wavelengths of 14 to 24 μm was calculated from FIG. 13 and was 91.7%.

[Example 2]
<1> Production of mineral functional water The mineral functional water of Example 2 was produced using the following raw materials and method.
1. Manufacture of Mineral-Containing Water (A) As a raw material of the mineral-giving material (A), as a plant plant material (A1), “P-101 (product number)” manufactured by Riken Technosystem Co., Ltd., as a wood plant material (A2) "P-201 (product number)" manufactured by Riken Techno System Co., Ltd. was used.
"P-101" is a plant material (A1) obtained by mixing the following dried and crushed Asteraceae plant and dried and crushed Rosaceae plant at a ratio of 1: 1 (weight ratio), and "P-201" is And woody plant materials (A2) described below.

(A1) Plant material (dried plant material)
(A1-1) Dry and Pulverized Asteraceous Plant Wild thistle (leaves, stems and flowers), mugwort (leaves and stems), and camellia (leaves and stems) were each 10% by weight, 60% by weight. What was mixed at a ratio of 30% by weight, dried, and then pulverized.
(A1-2) Dry and Pulverized Rosaceae Plants Wild rose (leaves and flowers), radish (leaves and stems), and raspberry (leaves, stems and flowers) were each 20% by weight, 10% by weight, What was mixed at a ratio of 70% by weight, dried, and then pulverized.
(A2) Woody plant material (dried woody plant)
Maple (leaf), birch (leaf, stem, and bark) and cedar (leaf, stem, and bark) are mixed at a ratio of 20% by weight, 60% by weight, and 20% by weight, respectively, and dried. After crushing.

  Example 2 was prepared in the same manner as in Example 1 except that the mineral-giving material (A) obtained by mixing the plant material (A1) and the woody plant material (A2) at a ratio of 1: 5 (weight ratio) was used. Of mineral-containing water (A).

2. Production of Mineral-Containing Water (B) Description is omitted because it is common to Example 1.

The mineral-containing water (A) and the mineral-containing water (B) of Example 2 formed by the above method were mixed at a ratio of 1:10 (weight ratio) to obtain a mineral-functional water of Example 2.
The mineral functional water of Example 2 corresponds to the mineral functional water A20ACA-717 (Terra Support (trade name), A20ACA-717 (product number)) manufactured by Riken Technosystem Co., Ltd.

<2> Evaluation of Spectral Emissivity FIG. 14 shows the results of evaluation of the spectral emissivity of the ceramic sintered bodies of Example 2 and the control sample by the same method as in Example 1 described above.

[Evaluation for prion protein]
<1> Evaluation by Direct ELISA Method The effect on the prion protein (PrP) of the mineral functional water of the example was evaluated by the Direct ELISA method based on the amount of change before and after the mineral functional water treatment on the test subject. The materials used for the evaluation and the evaluation method are as follows.

(Sample to be measured)
・ Mouse Recombinant PrP (Jena Bioscience)
・ PrP in the brain of 263K strain hamster infected with scrapie (pellet state)
(Treatment solution)
・ MC Water (CA-SI-01)
・ MC water (CA-C-01, main component: carbon, calcium)
・ Block Ace (control)
“CA-C-01” is the mineral water of Example 1, “CA-SI-01” is the mineral water of the reference example, and “Black Ace” is the control. CA-SI-01 manufactured by Riken Technosystem Co., Ltd. (development product number) is a mineral functional water containing silicon as a main component, and has a pH of 10.

(Method)
First, a sample was adsorbed to a plate, and nonspecific reaction was blocked with Block Ace. Then, it was washed with TBST and MC water was added. After washing with TBST, 6H4-HRP antibody was added. Further, after washing with TBST, TMB was added and reacted. After terminating the reaction by adding a reaction terminating solution, the absorbance was measured at a wavelength of 450 nm.
In addition, in order to perform quantification, a dilution series of known PrP was prepared, and a significant difference between treatment groups was examined by a t-test.

  FIG. 15 shows the change in the amount of mouse recombinant PrP before and after the treatment with mineral water. FIG. 16 shows the change in the amount of abnormal prion in hamsters infected with scrapie.

  From FIG. 15 and FIG. 16, it was confirmed that the mineral-functional water of Example 1 was able to significantly reduce the amounts of mouse recombinant PrP and scrapie-infected hamster abnormal prions. From this, it was confirmed that the abnormal prion protein can be at least partially degraded or denatured by treating with mineral-functional water.

<2> Evaluation by Western Blotting Method 200 μL of brain homogenate containing 200 μg protein (263K infected hamster brain) and 20 μL of MC water (CA-C-01, main component: carbon, calcium) were mixed and reacted at room temperature for 1 hour. Was. Next, 20 μg / mL of proteinase K was added, and the mixture was treated at 37 ° C. for 1 hour. By treating with proteinase K, normal prion protein (PK-sensitive PrP) is degraded, and thus the obtained sample contains only abnormal prion protein. The obtained sample was evaluated by Western blotting.
As a control, a sample prepared by performing the same operation using 200 μL of a brain homogenate (normal hamster brain, uninfected) containing 200 μg of protein instead of 200 μL of brain homogenate (263K infected hamster brain) was prepared and evaluated by Western blotting. did.

  In addition, a sample not treated with proteinase K was similarly evaluated by Western blotting. The sample not treated with proteinase K is a sample containing an abnormal prion protein (PK-resistant PrP) and a normal prion protein (PK-sensitive PrP).

The results are shown in FIG. 17 and FIG. FIG. 17 shows the result of 6 μL / each Lane, and FIG. 18 shows the result of 3 μL / each Lane. PK (+) and PK (-) in the figure represent the presence or absence of proteinase K treatment.
As shown in FIGS. 17 and 18, the samples treated with MC water had reduced abnormal prion protein, but had no effect on normal prion protein. From this, it was confirmed that the contact between the abnormal prion protein and the mineral-functional water allowed the overall degradation of the abnormal prion protein and reduced the infectivity of the abnormal prion protein.

<3> Evaluation in vivo A total of five 263K strain Syrian hamsters, 8 weeks old, males were used.
First, a scrapie-infected hamster 10% brain emulsion and MC water (CA-C-01 or CA-SI-01) were mixed at a ratio of 1: 1 and allowed to stand for 1 hour. As shown in Table 1, 100 μL of the obtained solution was inoculated into Syrian hamsters in the brain.
For individuals showing the symptoms of scrapie (positive control, hamster numbers P-1, P-2), brains were collected 62 days or 64 days later. Brains were collected 72 days later from individuals (hamster numbers S-1, S-2, S-3) in which no symptoms of scrapie appeared. The ingested brain tissue was stored at -80 ° C.

Next, after the brain tissue was thawed, the tissue embedded in 10% neutral buffered formalin and embedded in paraffin was sliced to prepare a tissue section. Tissue sections were immunostained using the anti-prion protein antibody 3F4 as a primary antibody, and then the accumulation and distribution of PrP ^Sc (abnormal prion protein) in the brain was observed by immunohistological examination.

  Table 2 shows the results of metastasis and immunohistological examination. 19 to 21 show tissue observation photographs of hamster numbers P-1, S-1, and S-3 shown in Tables 1 and 2.

As shown in FIG. 19, the P-1 individual (positive control) showed signs of scrapie, and brown PrP ^Sc was observed in the brain. The same was true for P-2.
On the other hand, as shown in FIG. 20, in the individual S-1, which was administered with the scrapie-infected hamster brain emulsion reacted with MC water (CA-SI-01), PrP ^Sc was observed in the brain, which was surprising. Fortunately, there were no signs of scrapie. The same was true for the individual S-2.
Furthermore, S-3, an individual to whom a scrapie-infected hamster brain emulsion that had been reacted with MC water (CA-C-01), did not show any signs of scrapie, and as shown in FIG. No PrP ^Sc was observed.

  From the above results, it is expected that prion molecules can be safely inactivated by using MC water.

  ADVANTAGE OF THE INVENTION According to this invention, the preventive treatment of the disease derived from abnormal prion protein can be performed.

Claims (6)

Abnormal prion protein, the infectivity of abnormal prion protein to reduce the infectivity of abnormal prion protein by contacting mineral functional water containing a mineral component that emits electromagnetic waves ,
The mineral functional water containing the electromagnetic wave-radiating mineral component comprises the mineral-containing water (A) formed in the following step (1) and the mineral-containing water (B) formed in the following step (2). A method for reducing infectivity of abnormal prion protein, characterized by being mineral-functional water obtained by mixing at a ratio of 1: 5 to 1:20 (weight ratio) (however, excluding medical practice for humans) .

Step (1):
A conductive wire covered with an insulator, a plant material consisting of a plant of the family Asteraceae and a plant of the family Rosaceae, and a woody plant comprising at least one woody plant selected from maple, birch, pine and cedar; And a mineral-imparting material (A) containing a plant material, immersed in water, conducting a direct current to the conductive wire, generating a water flow in the same direction as the direct current in the water around the conductive wire, A step of forming a raw mineral aqueous solution (A) by applying ultrasonic vibration to water, and then irradiating the raw mineral aqueous solution (A) with far infrared rays (wavelength 6 to 14 μm) to form a mineral-containing water (A) And
The amount of the mineral-imparting material (A) added to water is 10 to 15% by weight, and the current value and the voltage value of the direct current conducted to the conductive wire are in the range of 0.05 to 0.1 A and 8000 to 8600 V, respectively. And
The mineral imparting material (A)
As the plant material, wild thistle (leaves, stems and flowers), mugwort (leaves and stems), and camellia (leaves and stems) are respectively 8 to 12% by weight and 55 to 65% by weight. , 27-33% by weight, dried and crushed Asteraceae plant after being dried and crushed, and
17-23% by weight, 8-12% by weight, 65-75% by weight of wild rose (leaf, flower), radish (leaf and stem), and raspberry (leaf, stem and flower), respectively Using a dry and crushed Rosaceae plant that was mixed and dried at a ratio of
A plant material (A1) obtained by mixing the dry and crushed Asteraceous plant and the dry and crushed Rosaceae plant in a ratio of 1: 0.8 to 1: 1.2 (weight ratio);
As the woody plant raw materials, maple (leaves and stems), birch (leaves, stems, and bark), cedars (leaves, stems, and bark) are each 22 to 28% by weight, A woody plant raw material (A2) comprising a dry and crushed product obtained by mixing at a ratio of 22 to 28% by weight and 45 to 55% by weight, drying, and then crushing the mixture.
Step of being a mineral-imparting material (A ′) obtained by mixing the plant material (A1) and the woody plant material (A2) in a weight ratio of 1: 2.7 to 1: 3.3.

Step (2):
Mineral-containing water (B) filled with different kinds of inorganic mineral-imparting materials, and water is passed through six water-passing containers from a first water-passing container to a sixth water-passing container connected in series, and mineral-containing water (B) is a step of forming
In the six water containers,
The mineral-imparting material (B1) in the first water passage container contains 65 to 75% by weight, 12.5 to 17.5% by weight, and 12.5 to 17.5% by weight of limestone, fossil coral, and shell, respectively. mixture,
The mineral-imparting material (B2) in the second water passage container contains limestone, fossil coral, shells, and activated carbon in an amount of 37 to 43% by weight, 12.5 to 17.5% by weight, 37 to 43% by weight, and 2.5%, respectively. A mixture comprising ~ 7.5% by weight;
The mineral-imparting material (B3) in the third water-passage container contains limestone, fossil coral, and shells in 75 to 85% by weight, 12.5 to 17.5% by weight, and 2.5 to 7.5% by weight, respectively. mixture,
The mineral-imparting material (B4) in the fourth water passage container contains 85 to 95% by weight, 2.5 to 7.5% by weight, and 2.5 to 7.5% by weight of limestone, fossil coral, and shell, respectively. mixture,
The mineral-imparting material (B5) in the fifth water passage container contains limestone, fossil coral, and shells respectively in 75 to 85% by weight, 7.5 to 12.5% by weight, and 7.5 to 12.5% by weight. mixture,
A mixture in which the mineral imparting material (B6) in the sixth water passage container contains 55 to 65% by weight, 27 to 33% by weight, and 7.5 to 12.5% by weight of limestone, fossil coral, and shells;
The process that is Abnormal prion protein, the infectivity of abnormal prion protein to reduce the infectivity of abnormal prion protein by contacting mineral functional water containing a mineral component that emits electromagnetic waves ,
A method for reducing infectivity of an abnormal prion protein , wherein the mineral component is a mineral component derived from mineral-functional water CAC-717 manufactured by Riken Technosystem Co., Ltd. (however, excluding medical practices for humans) . Abnormal prion protein, the infectivity of abnormal prion protein to reduce the infectivity of abnormal prion protein by contacting mineral functional water containing a mineral component that emits electromagnetic waves ,
A method for reducing infectivity of an abnormal prion protein , wherein the mineral component is a mineral component derived from a mineral functional water A20ACA-717 manufactured by Riken Technosystem Co., Ltd. (however, excluding medical practices for humans) . The method according to any one of claims 1 to 3, wherein the mineral component is a mineral component that emits an electromagnetic wave including a terahertz wave. It is a pharmaceutical composition for the prevention and treatment of prion disease, comprising as an active ingredient mineral functional water containing an electromagnetic wave emitting mineral component ,
A pharmaceutical composition for preventing or treating prion disease, wherein the mineral component is a mineral component derived from mineral functional water CAC-717 manufactured by Riken Technosystem Co., Ltd. It is a pharmaceutical composition for the prevention and treatment of prion disease, comprising as an active ingredient mineral functional water containing an electromagnetic wave emitting mineral component ,
A pharmaceutical composition for preventing or treating prion disease, wherein the mineral component is a mineral component derived from a mineral functional water A20ACA-717 manufactured by Riken Technosystem Co., Ltd.