JP6608217B2 - Solid composition - Google Patents

Description

  The present invention relates to a solid composition containing a plant-derived protein and / or peptide and a foaming component.

Many solid products that use a mechanism in which carbon dioxide gas is generated by the neutralization reaction of carbonate and organic acid are on the market. One of them is oral solid preparations such as effervescent tablets and expanded granules, and confectionery such as tablet confectionery. Solid preparations containing foaming ingredients are not only excellent in portability and storage, but are also popular because they can foam when contacted with saliva and water in the mouth and give a unique refreshing sensation in the oral cavity. It is.
It is also known that the foaming component facilitates ingestion of a solid composition containing a high concentration of wheat albumin when combined with wheat albumin which is a water-soluble protein (Patent Document 1).

As another use of the foamed preparation, there is a gastrointestinal expansion agent for diagnosing gastrointestinal tract in which foaming components are not foamed in the mouth but are foamed in the digestive tract such as the stomach after ingestion. This gastrointestinal dilator is designed to defoam immediately after foaming in the stomach so as not to interfere with contrast.
On the other hand, carbon dioxide gas (bubbles) is quickly generated in the stomach, and when the stomach is inflated, it is known that the center of satiety of the brain is stimulated, resulting in abdominal (stomach) fullness and fullness ( Non-patent document 1).

JP 2013-87062 A

J Nutr Sci Vitamol, 2012, Vol. 58, No. 5, p. 333-338

However, since the above-mentioned gastrointestinal dilator is used for the purpose of assisting in imaging of the gastrointestinal tract and projection, a large amount of foam is required promptly. Therefore, it contains a very large amount of foaming ingredients and is not suitable for daily intake or continuous intake.
On the other hand, in order to improve foaming in the stomach, it is necessary to consider that the saliva is almost neutral, whereas the gastric juice is strongly acidic (pH 1.0-2.0). However, a solid composition having a good flavor that is excellent in bubbling in the stomach and suitable for continuous oral intake on a daily basis has not been known.
Control foaming after ingestion and improve foaming in the stomach, for example, to give a feeling of fullness and satiety, prevent early eating, and control food intake It is considered useful.
Accordingly, the present invention relates to providing a solid composition having excellent foaming in the stomach and good flavor.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors combined foaming components with plant-derived proteins and / or peptides that have a large amount of insoluble components, so that foaming in the stomach is good. Moreover, it discovered that the balance of a sour taste and a bitter taste was taken by containing carbonate and an organic acid in a predetermined ratio, and it can be set as a solid composition with favorable flavor.

That is, the present invention includes the following components (A), (B) and (C):
(A) a plant-derived protein and / or peptide having an insoluble component amount of 25% by mass or more,
(B) carbonate 5-30% by mass,
(C) Organic acid 5-30 mass%
And a solid composition having an equivalent ratio of component (C) to component (B) [equivalent of (C) / equivalent of (B)] of 0.4 to 4.

  According to the present invention, it is possible to provide a solid composition excellent in foaming in the stomach and having a good balance between acidity and bitterness.

The (A) plant-derived protein and / or peptide used in the present invention has an insoluble component amount of 25% by mass (hereinafter, simply referred to as “%”) or more. By setting it as this range, the amount of foam generated in the stomach can be increased.
The amount of insoluble components can be measured by the following method.
[Method for measuring the amount of insoluble components]
Collect plant-derived protein and / or peptide in a container. Add 20 times the amount of water collected and shake for 20 minutes. After shaking, the mixture is centrifuged at 3000 r / min for 20 minutes, the supernatant is removed, and the precipitate is collected. This operation was repeated three times, and the final recovered precipitate was freeze-dried and measured as the amount of insoluble components.

The insoluble component is a component hardly soluble in water, and is mainly a water-insoluble protein and / or a water-insoluble peptide. In the solid composition of the present invention, the nitrogen content of the insoluble component contained in the plant-derived protein and / or peptide is preferably 10% or more, and more preferably 10 to 16%.
The nitrogen content can be measured by the following method.
[Method for measuring nitrogen content]
Measuring device: Oxygen circulation combustion / TCD detection method NCH quantification device Sumigraph NCH-22F type Measurement conditions: Reaction temperature 850 ° C
・ Reduction temperature 600 ℃
Separation / detection Porous polymer bead packed column / TCD
Standard sample Elemental determination standard sample Acetanilide

(A) Examples of plant-derived proteins include soy protein, pea protein, wheat protein, rice protein, and the like.
Examples of the (A) plant-derived peptide include hydrolysates and enzyme-decomposed products of the plant-derived proteins, products obtained by fermentation, and peptides purified or isolated from them.
Of these, soy protein, wheat protein, and rice protein are preferred because of high protein content, and soy protein is particularly preferred.
The molecular weight of proteins and peptides is not particularly limited.

  (A) The plant-derived protein and / or peptide can be obtained by, for example, extracting from a plant using a known method, and the extraction conditions can also be set as appropriate. Commercial products can also be used.

(A) Plant origin protein and / or peptide can be used by 1 type or in combination of 2 or more types. In the solid composition of the present invention, the content of the (A) plant-derived protein and / or peptide having an insoluble component amount of 25% or more is 3.5 from the point that the amount of foam generated in the stomach is large. % Or more, preferably 10% or more. In the solid composition, the content of (A) plant-derived protein and / or peptide having an insoluble component amount of 25% or more is 70% or less, further 50% or less, and further 25% or less. preferable.
In the present specification, the content of protein and / or peptide is the amount of one of protein and peptide when it contains only one, and the total amount when it contains both. Further, the protein and / or peptide content is defined as an N-equivalent protein amount that can be measured according to the method described in the Examples below.

In addition to the component (A), the solid composition of the present invention may contain proteins and / or peptides other than the component (A), but the amount of foam generated in the stomach is large. In view of this, the nitrogen content of insoluble components in the total protein and / or total peptide in the solid composition is preferably 10 to 20%.
Further, the nitrogen content of the insoluble component in the solid composition is preferably 0.3 to 15%.

The solid composition of the present invention contains (B) carbonate and (C) organic acid as foaming components.
Examples of the carbonate (B) used in the present invention include sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, calcium carbonate, magnesium carbonate, sodium sesquicarbonate and the like. Among these, sodium carbonate and sodium hydrogen carbonate are preferable, and sodium hydrogen carbonate is more preferable because the amount of foam generated in the stomach is large and the texture is good.

  (B) As carbonate, it can be used by 1 type or in combination of 2 or more types. In the solid composition of the present invention, the content of (B) carbonate is 5 to 30% from the viewpoint that the amount of foam generated in the stomach is large and bitterness is reduced. From the same point, the content of (B) carbonate in the solid composition is preferably 10 to 25%, and more preferably 10 to 20%.

As the organic acid (C) used in the present invention, an edible acid can be used. Examples thereof include organic acids such as citric acid, succinic acid, ascorbic acid, acetic acid, gluconic acid, malic acid, tartaric acid, fumaric acid and adipic acid.
Among these, citric acid or malic acid is preferable, and citric acid is more preferable from the viewpoint that an appropriate sourness is obtained in the oral cavity.

  (C) As an organic acid, it can be used 1 type or in combination of 2 or more types. In the solid composition of the present invention, the content of (C) the organic acid is 5 to 30% from the point that the amount of foam generated in the stomach is large and a proper acidity is felt. From the same point, the content of the (C) organic acid in the solid composition is preferably 10 to 27%, more preferably 10 to 25%.

  Further, in the solid composition of the present invention, the total content of (B) carbonate and (C) organic acid is 10 to 10 in that the amount of foam generated in the stomach is large and bitterness is reduced. 60%, further 20 to 50%, and further 30 to 50% are preferable.

In the solid composition of the present invention, the equivalent ratio of the component (C) to the component (B) [equivalent of (C) / equivalent of (B)] is 0.4 to 4 in terms of the balance of flavor. .
In the present specification, the “equivalent ratio” is a value obtained by dividing the equivalent of (C) organic acid contained in the solid composition by the equivalent of (B) carbonate.
The equivalent ratio of the component (C) to the component (B) [equivalent of (C) / equivalent of (B)] is 0.6 to 3.5, more preferably 0.9 to 1.5 from the viewpoint of the balance of flavor. Is preferred.

  In this invention, content of the plant-derived protein and / or peptide whose (A) insoluble component amount in a solid composition is 25 mass% or more with respect to content of (B) carbonate in a solid composition. The ratio (containing mass ratio) [(A) / (B)] is 0.3 to 5, more preferably 0.6 to 5, and more preferably 1 to 5 in terms of the amount of foam generated in the stomach Is preferred.

  In the solid composition of the present invention, in addition to the above components, minerals (for example, calcium, magnesium, iron, zinc, chromium, selenium, manganese, molybdenum, copper, iodine, Phosphorus, potassium, sodium), vitamins (eg, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin E, folic acid and their salts, or esters thereof), sweeteners (eg, fructose) Monosaccharides such as glucose, galactose and xylose; oligosaccharides such as sucrose, lactose, maltose, trehalose, isomaltoligosaccharide, galactooligosaccharide, fructooligosaccharide, dairy oligosaccharide, soybean oligosaccharide, isomaltulose and coupling sugar Sugar alcohol, saccharin, sucralose, stevia, a Synthetic sweeteners such as sul Fam potassium), surfactant, component (C) other than the acidulant, flavor, coloring agents, preservatives, etc. may be appropriately blended.

  As a form of the solid composition of this invention, a solid thing is meant at room temperature (15-25 degreeC), For example, solid forms, such as a powder, solid, a granule, are mentioned. In addition, specific preparations (dosage forms) include capsules, granules, powders, tablets, pills, troches and the like. Among these, tablets, granules, powders, pills, and lozenges that are effervescent tablets are preferable from the viewpoint of easy intake and intake as food, and tablets, granules, A powder is more preferable.

  To prepare the solid composition, an acceptable carrier can be blended as necessary. For example, excipients (eg, starches, crystalline cellulose, light anhydrous silicic acid, calcium hydrogen phosphate, etc.), binders (eg, hydroxypropylmethylcellulose, hydroxypropylcellulose, gelatin, pregelatinized starch, polyvinylpyrrolidone, polyvinyl alcohol , Pullulan, methylcellulose, hydrogenated oil, etc.), disintegrating agents (eg, carmellose, carmellose calcium, croscarmellose sodium, crospovidone, corn starch, low substituted hydroxypropylcellulose, etc.), lubricants (eg, calcium stearate, Magnesium stearate, sodium stearyl fumarate, talc, silicon dioxide and the like), fillers, dispersants, buffers, diluents and the like.

The solid composition of the present invention generates carbon dioxide in the presence of saliva or water in the mouth. In particular, after oral ingestion, it reacts with water in the stomach and quickly foams, and is excellent in foaming in the stomach. Therefore, the solid composition of the present invention is suitable as a product or the like that gives an early feeling of fullness or fullness, prevents early eating, and controls food intake.
The solid composition of the present invention is preferably taken orally at the time of feeding or before feeding, and more preferably taken within 5 to 30 minutes before feeding, from the viewpoint of effectively exerting the effect.
The pH of gastric juice is generally 1.0 to 2.0, although the acidity varies depending on the situation.

In the solid composition of the present invention, the measured value (mL) of the amount of foam after 1 minute by foaming evaluation using a gastric juice model described in Examples described later is 8 mL or more, and further 11 mL or more. preferable.
In addition, the solid composition of the present invention preferably has a relative foam amount (%) of 30% or more after 1 minute according to foaming evaluation using a gastric juice model described in Examples described later. It is preferable that it is 40% or more. It can be judged that the larger this value, the better the foaming in the stomach.

The solid composition of the present invention is produced according to a conventional method without any particular limitation.
For example, when producing a powder, (A) a plant-derived protein and / or peptide having an insoluble component amount of 25% or more, (B) a carbonate, (C) an organic acid, and additives added as necessary And the mixture may be used as it is, or the mixture may be pulverized. The powder preferably passes through the entire size of No. 18 (850 μm) sieve, and the amount remaining on the No. 300 (500 μm) sieve is more preferably 5% or less of the total amount.
The granule is prepared by mixing (A) a plant-derived protein and / or peptide having an insoluble component amount of 25% or more, (B) carbonate, (C) an organic acid, and an additive added as necessary. The mixture can be obtained by granulation using a dry granulation method, a wet granulation method or the like. Examples of the granulation method include extrusion granulation method, crushing granulation method, rolling granulation method, stirring granulation method, fluidized bed granulation method and the like. The average particle diameter of the granulated product is preferably 45 μm to 850 μm, and more preferably 100 μm to 500 μm.
When manufacturing a tablet, the raw material powder may be directly compressed and molded, or granulated using the dry granulation method, wet granulation method, etc., and then the granulated product may be compressed and molded. good.
When a tablet is manufactured by directly or compressing the granulated product, a tableting machine such as a rotary tableting machine or a single-shot tableting machine can be used.
The weight per tablet is preferably 0.05 to 3 g from the viewpoint of simplicity and effectiveness.

[Protein analysis]
The mass of the protein was calculated by multiplying the nitrogen amount determined by the combustion method by a nitrogen-protein conversion factor. Nitrogen-protein conversion factors are milk: 6.38, egg white: 6.25, rice: 5.95, wheat: 5.7, soybean: 5.71.
Nitrogen-protein conversion factor used for the calculation of nitrogen amount is based on Table 7 of the Ministry of Education, Culture, Sports, Science and Technology's 5th revised Japanese food standard composition table, Chapter 1 2) Listed component items, etc. Conversion factors for foods other than those were used.

[Analysis of carbonate]
The method for analyzing the content of carbonate in the solid composition is as follows.
0.1 to 0.2 g of a solid composition was sampled, and 10 mL of water and 2 mL of 50% phosphoric acid were added and sealed. After sonication for 10 minutes, one hour left headspace gas determine the amount of CO ₂ was subjected to gas chromatography, was calculated from the generated amount of CO _2.
<Gas chromatograph operating conditions>
Model: GC-14B [Shimadzu Corporation]
Detector: TCD
Column: Chromosorb 101, 80-100 mesh
Glass tube, φ3.2mm × 2m
Temperature: Column 50 ° C, inlet and detector 100 ° C
Cell current 75mA
Gas pressure: Helium (carrier gas) 100 kPa
Injection volume: 0.2 mL of headspace gas

[Analysis of organic acids]
The analysis method of the content of the organic acid in the solid composition is as follows.
1 g of the solid composition was sampled, 20 mL of 5% perchloric acid was added, and the mixture was extracted by shaking for 10 minutes. This was made up to 200 mL with water and sonicated for 10 minutes. It used for the high performance liquid chromatograph after filtration.
<High-performance liquid chromatograph operating conditions>
Model: LC-20AD [Shimadzu Corporation]
Detector: UV-visible spectrophotometer SPD-20AV [Shimadzu Corporation]
Column: Shim-pack SCR-102H 300 × 80 (length × inner diameter (mm)) [Shimadzu Corporation]
Column temperature: 40 ° C
Mobile phase: 3 mmol / L perchloric acid reaction solution: 0.2 mmol / L bromthymol blue-containing 15 mmol / L disodium hydrogen phosphate solution Flow rate: mobile phase 1.0 mL / min, reaction solution 1.4 mL / min
Measurement wavelength: 445 nm

[Raw materials] Raw materials having the origin and composition shown in Table 1 were used as proteins or peptides.
P-1: NUTRALYS W (Rocket Japan Co., Ltd.)
P-2: NUTRALYS F85M (Rocket Japan Co., Ltd.)
P-3: Ripro80 (Oryza Oil Co., Ltd.)
P-4: SUPRO XT 219D IP (DuPont)
P-5: Harmony A100 (Nippon Shinyaku Co., Ltd.)
P-6: Milk protein (carbohydrate: 11.3%, lipid: 25.1%, protein: 54.1%, moisture: 4.9%, ash: 4.6%)
P-7: WGE80GPU (Nippon Shinyaku Co., Ltd.)

The following raw materials were used.
Sodium bicarbonate: baking soda (food additive C), Tosoh citric acid: anhydrous citric acid MS, Fuso Chemical Industry Co., Ltd.
Malic acid: Fuso S, Fuso Chemical Industry Co., Ltd.
Calcium stearate: Orlabrite CA-65, NOF Corporation
Maltitol: Amarti MR-50, Mitsubishi Corporation Foodtech Co., Ltd.

Test Examples 1-7 ( Test Examples 4 and 5 are reference examples)
Preparation of solid shape Composition
Each raw material component was mixed with the composition shown in Table 2 to obtain a powdery composition. The parentheses in the component (A) indicate the origin of the protein. Table 2 shows the ratio of the content of the component (A) to the content of the protein and the content of the component (B) (content mass ratio) in each composition.

[Evaluation of foam amount]
The amount of foam in the gastric juice model was evaluated as follows for the product of the present invention and the comparative product obtained above.
(1) Definition and calculation of theoretical amount of foam per gram The number of moles of carbon dioxide produced by the reaction of carbonate and organic acid in the solid composition was obtained by dividing the mass of carbonate by 1 gram equivalent of carbonate. It is the smallest value (Mi) among the value and the value obtained by dividing the mass of the organic acid by 1 gram equivalent of the organic acid. On the other hand, the volume of 1 mol of ideal gas is 22.7 liters under standard conditions (0 ° C., 1 bar). Therefore, in the present specification, the value obtained by multiplying the Mi by 22.7 liters is defined as the theoretical amount of foam (mL) per 1 g of the solid composition.

(2) Gastric juice model solution Disintegration test first solution (pH 1.2) based on the 16th edition revised Japanese Pharmacopoeia was used as a gastric juice model solution (Kanto Chemical Co., Ltd.).

(3) Definition and calculation of relative foam amount The actual foam amount (measured amount) (mL) generated from 1 g of the solid composition poured into the gastric juice model solution is the theoretical foam amount (mL) per 1 g of the solid composition. The value expressed as a percentage was taken as the relative foam amount (%).
Relative foam amount (%) = [actually measured amount (mL)] / [theoretical bubble amount per 1 g (mL)] × 100
The measured amount (mL) was obtained by putting 1 g of a sample into a 50 mL graduated cylinder, adding 2 mL of the above gastric juice model solution, taking 0 minute immediately after the addition, and reading the scale at the top of the foam after 1 minute.
Table 2 shows the actually measured amount (mL) and the relative bubble amount (%) after 1 minute for each of the product of the present invention and the comparative product.

〔sensory evaluation〕
The sensory evaluation was performed on the product of the present invention and the comparative product obtained above by three specialized panels. The evaluation was performed according to the following criteria for the balance between sourness and bitterness felt when eating a sample, and the score was determined through consultation. The results are shown in Table 2.
(Balance between acidity and bitterness)
5: Strong bitterness 4: Slightly strong bitterness 3: Good balance between acidity and bitterness 2: Slightly strong acidity 1: Strong acidity

Test Examples 8 to 23 ( Test Examples 16 and 17 are reference examples)
Preparation of solid shape Composition
Each raw material component was mixed with the composition shown in Table 3 to obtain a powdery composition. The parentheses in the component (A) indicate the origin of the protein. Table 3 shows the amount of protein in each composition, the ratio of the content of component (A) to the content of component (B) (content mass ratio), and the equivalent ratio of component (C) to component (B). . Test Example 23 is shown in Table 4.

Test Example 24
(Preparation of solid composition)
Each raw material component was mixed with the composition shown in Table 4. Next, uncoated tablets were obtained using a single-type tableting machine (manufactured by RIKEN).

[Evaluation of foam amount]
The present invention and the comparative product obtained above were evaluated in the same manner as the amount of foam. The measured amount (mL) and the relative bubble amount (%) after 1 minute are shown in Tables 3 and 4.

〔sensory evaluation〕
About this invention product obtained above and a comparative product, the balance of the acidity and bitterness which are felt when eating a sample was similarly evaluated. The results are shown in Tables 3 and 4.

As is apparent from Tables 2 to 4, the product of the present invention in which a plant-derived protein having a predetermined amount of insoluble components is blended is less foamed than a comparative product in which animal-derived proteins are blended or a comparative product having a small amount of insoluble components. It was confirmed that it was good.
The product of the present invention had a good flavor with little bitterness while having an appropriate acidity.

Claims (6)

The following components (A), (B) and (C):
(A) Wheat protein or rice protein having an insoluble component amount of 25% by mass or more,
(B) carbonate 5-30% by mass,
(C) Organic acid 5-30 mass%
A solid composition for oral administration in which the equivalent ratio of component (C) to component (B) [equivalent of (C) / equivalent of (B)] is 0.4-4. The oral solid composition according to claim 1, wherein the nitrogen content of the insoluble component (A) is 10 to 16% by mass. The oral solid composition according to claim 1 or 2, wherein the content of the component (A) is 3.5 to 70% by mass. The solid composition for oral administration according to any one of claims 1 to 3, wherein the mass ratio [(A) / (B)] of the component (A) and the component (B) is 0.3 to 5. Component (C) is a citric acid, malic acid, or these combination, The oral solid composition of any one of Claims 1-4. It is an effervescent tablet or an expanded granule, The solid composition for oral administration of any one of Claims 1-5.