JP6606352B2 - Determination method of ethanol and glucose in moromi and filtration device - Google Patents

Description

  The present invention relates to a method for quantifying ethanol and glucose in moromi and a filtration device.

  The present invention also relates to a method for correcting the influence of Raman scattering peaks due to turbidity and / or coloring.

  Conventionally, in the brewing process of sake and the like, the alcohol content is quantified by gas chromatography, but the burden on time and cost is large. In addition, the sugar content may be simply measured using a diabetes meter for diabetics, but the investigation by the present inventors has revealed that the cost of consumables is high and there is a problem in measurement accuracy. Yes. On the other hand, measuring instruments utilizing near-infrared spectroscopic measurement are sold for alcohol content (for example, alcoholicizer TS for sake made by Anton Paar, http://www.k-tsukamoto.co.jp/htmls/s_bunseki) /Images/alcolyzer.pdf).

  However, near-infrared spectroscopic measurement has inherently low spectral resolution, so accurate identification and quantification of each component in a solution containing multiple components is difficult, and is affected by temperature and other atmospheric conditions. There is a problem that the apparatus is large and the portability is poor, the sample volume is relatively large as 30 mL, and the cost is high. In particular, it has been confirmed by the inventor's original examination that the alcohol quantitative value by near-infrared spectroscopic measurement is significantly affected by the coexistence of components such as starch, which causes a decrease in the reliability of the measurement results. .

  Non-Patent Document 1 individually measures the glucose concentration in the cellulose saccharification process and the ethanol concentration in the fermentation process by Raman spectroscopy, and Non-Patent Document 2 analyzes the content of various sugars in soft drinks. Document 3 discloses a method for quantifying ethanol, acetone, and methanol dilute aqueous solution by Raman spectroscopy, and Non-Patent Document 4 analyzes the process of ethanol fermentation from glucose in situ by Raman spectroscopy.

Determination of glucose and ethanol after enzymatic hydration and fermentation of biomass using Raman spectroscopy, Chien-Ju Shih, Emily. Smith, Analytica Chimica Acta 653 (2009) 200-206. Visible micro-Raman spectroscopy for determining glucose content in beverage industry, I.I. Delfino, C.I. Cameringo, M.M. Portaccio, B.M. Della Ventura, L.A. Mita, D.D. G. Mita, M.M. Lepore, Food Chemistry 127 (2011) 735-742 The Direct Analysis of Fermentation Products by Raman Spectroscopy, THOMAS B. SHOPE, THOMAS J. et al. Vickers, and CHARLES K. MANN, APPLIED SPECTROCOPY, Volume 41, Number 5, 1987, 906. Noninvasive, On-Line Monitoring of the Biotransformation by Yeast of Glucose to Ethanol Usage Dispersive Raman Spectroscopy and ChemimetricRI. SHAW, NAHEED KADERBHAI, ALUN JONES, ANDREW M. WOODWARD, ROYSTON GOODACCRE, JEM J. ROWLAND, and DOUGLAS B.R. KELL, APPLIED SPECTROCOPY, Volume 53, Number 11, 1999, 1419.

  An object of the present invention is to provide a simple and accurate method for quantifying ethanol and glucose in moromi and a filtration device.

  In the brewing process of sake, etc., it is necessary to measure the alcohol content and sugar content as needed for process control and product quality control. The present inventors can measure the alcohol and glucose concentrations contained in moromi during the alcoholic beverage production process easily, quickly, accurately, and at low cost by using a Raman spectroscopic device and a filtration method devised uniquely. I found a new method.

The present invention provides the following ethanol and sugar determination method and filtration device, and a method for correcting the influence of the Raman scattering peak.
Item 1. Including the step of filtering the mash of the moromi by one-stage filtration or multi-stage filtration, and the step of simultaneously quantifying the ethanol concentration and glucose concentration in the obtained filtrate with a Raman spectroscopic device; Simultaneous determination method of glucose concentration.
Item 2. Item 2. The method for simultaneous determination of ethanol concentration and glucose concentration in mash according to item 1, comprising a step of filtering mash using filter paper and a filter having a pore size of 5 µm or less.
Item 3. Item 2. The method for simultaneous determination of ethanol concentration and glucose concentration in mash according to item 1, comprising a step of filtering mash using filter paper and a filter having a pore size of 1 µm or less.
Item 4. Item 4. The method for simultaneous quantification of ethanol concentration and glucose concentration in mash according to Item 3, further comprising a step of filtering the mash with a filter paper and further performing two-stage filtration using a filter having a pore size of 1 µm or less.
Item 5. Item 5. The method for simultaneous determination of ethanol concentration and glucose concentration in a mash according to Item 3 or 4, wherein the filter is a filter having a pore size of 0.45 µm or less.
Item 6. Item 6. The method for simultaneously quantifying the ethanol concentration and glucose concentration in the mash according to any one of Items 1 to 5, wherein the excitation light of the Raman spectroscopic device is excitation light in the near infrared region.
Item 7. Item 7. A method for simultaneous determination of ethanol concentration and glucose concentration in mash according to Item 6, wherein the excitation light is 785 nm laser light.
Item 8. Item 8. The method for simultaneous determination of ethanol concentration and glucose concentration in mash according to any one of Items 1 to 7, wherein the Raman spectroscopic device is a portable device.
Item 9. Item 9. The method for simultaneously quantifying ethanol concentration and glucose concentration in mash according to any one of items 1 to 8, wherein the mash is mash of sake, shochu, awamori or makgeolli.
Item 10. A filtration device for quantifying the concentration of ethanol and glucose in moromi,
A pressure load container for extruding the mash while applying pressure from one side;
A first opening for the flow of the moromi loaded with pressure, a region for mounting the first filter for filtering the mash, and a liquid material filtered by the first filter are sent out. A first filter unit having a second opening and an attachment for mounting or replacing the first filter;
A first opening through which the liquid material fed from the second opening of the first filter unit flows, and further for filtering the liquid material, wherein the first filter And a second filter unit having a second filter unit having a region where a second filter having a smaller pore diameter is mounted and a second opening for delivering a liquid component filtered by the second filter. Filtration instrument.
Item 11. Item 11. The method for simultaneous determination of ethanol concentration and glucose concentration in mash according to Item 1, comprising a step of filtering solid components in the mash by two-stage filtration using the filtration device according to Item 10.

  According to the present invention, alcohol and glucose concentrations contained in moromi during the production process of alcoholic beverages such as sake can be measured easily, quickly, accurately and at low cost. Furthermore, it can be used for manufacturing process management of foods and various products containing alcohol and the like and quality control of products.

It is a Raman scattering spectrum figure (measurement time 10 seconds) of the mash sample which has not performed the filtration process, ethanol aqueous solution, and glucose aqueous solution. (A) It is a Raman scattering spectrum figure (measurement time 10 second) of the mash sample which filtered with a 0.45-micrometer filter, ethanol aqueous solution, and glucose aqueous solution. (B) It is a figure showing the correlation of the Raman scattering intensity (measurement time 10 second) in 872 cm < ^-1 > of the mash sample and ethanol aqueous solution which performed the filtration process with the 0.45 micrometer filter, and ethanol concentration. (A) Raman scattering spectrum diagram of glucose aqueous solution in the presence of 20 vol / vol% ethanol (measurement time 60 seconds). (B) It is a figure showing the correlation of the Raman scattering intensity (measurement time 60 second) in 1120cm < ^-1 > of the glucose aqueous solution in the coexistence of 20 vol / vol%, and glucose concentration. It is a Raman scattering spectrum figure (measurement time 10 second) of 872 cm < ^-1 > vicinity of the Makgeolli sample which performed various filtration processes. It is a figure which shows the structure of the two-stage filtration and measurement unit of this invention. One-stage filtration with a 0.45 μm filter, or No. It is a Raman scattering spectrum figure (measurement time 10 second) of 872 cm ^-1 vicinity of a sake sample on a laurel wreath subjected to two-stage filtration with 5A filter paper and a 0.45 μm filter.

  Raman spectroscopy is vibrational spectroscopy, and shows distinct peaks that are more separated compared to near-infrared spectroscopy, and is therefore characterized by the ease of identifying and quantifying specific components in complex mixtures. In addition, it has low sensitivity to water and is suitable for measurement in an aqueous solution.

  The present invention comprises a filtration technique and a technique for determining the concentration of each component. In the case of a sample in which the solid content is suspended in the mash, the present inventors have found that it is almost impossible to accurately measure the concentration of ethanol or glucose, and that the size is above a certain level by an appropriate filtration technique. It was found that accurate quantification would be possible by removing the solid matter.

  The moromi includes moromi of sake, shochu, awamori, makgeolli, etc., but the koji is prepared by attaching koji molds to raw materials such as rice, wheat (barley, wheat, etc.) and sweet potato, and then fermented with yeast. Any moromi obtained can be measured by the method of the present invention.

  As a filtration method for moromi, in order to remove solid matter, a filter having a pore size of 1 μm or less, for example, a filter having a pore size of 0.45 μm or less is preferably used, and one or more types of filters are selected. Alternatively, multistage filtration is performed. In particular, the time and labor required for filtration can be greatly reduced by employing a two-stage filtration system. When the mash is filtered through a filter having a pore size of 0.45 μm or less, it takes a very long time to filter due to clogging. For example, the mash is filtered with a filter paper to filter large solids, then filtered with a filter with a pore size of 5 μm, and further filtered with a filter with a pore size of 1 μm or less (for example, a pore size of 0.45 μm). Then, by filtering with a filter having a pore size of 1 μm or less (for example, a pore size of 0.45 μm), the time required for filtration can be greatly shortened, and more accurate quantitative measurement can be realized.

  Although the measured value can be obtained even by mashing before the filtration by the Raman spectroscopic device, the present inventor has confirmed that this measured value has no correlation with the ethanol concentration. In a filtrate obtained by filtering mash with a filter paper, the correlation between the ethanol concentration and the measured value by the Raman spectroscopic device may be insufficient depending on the mash sample, and the measurement error may increase. By filtering with a filter having a pore size of 1 μm or less (for example, a pore size of 0.45 μm), such a measurement error is suppressed to an allowable range. Filtration with a filter paper may be performed by natural filtration at normal pressure, but may be performed by vacuum filtration or pressure filtration. For example, a filter unit containing filter paper inside is connected to the tip of the syringe, and the filtrate is drawn into the syringe by pulling the plunger, and then the filter unit is replaced with a filter unit with a pore size of 1 μm or less (for example, 0.45 μm pore size). Then, two-stage filtration can be performed by pushing the plunger.

  Depending on the filter material, the filter can be reused by washing. Alternatively, the filter can be changed after each filtration. For example, when using a filter unit including filter paper as a filter, it can be used repeatedly by exchanging the filter paper. A filter unit including a filter having a pore size of less than 1 μm, such as a membrane filter, can be used a plurality of times as long as it is not clogged. However, a disposable filter unit that does not replace the filter is preferable from the viewpoint of improving work efficiency.

  The pore size of the filter is preferably 0.45 μm or less, but a pore size of 0.025 μm or more is preferable so as not to increase the filtration time. Furthermore, a pore diameter of 0.20 μm or more is more preferable.

  A portable handheld Raman spectrometer is available on the market. Using a handheld Raman spectrometer can easily determine the concentration of ethanol and glucose at the brewing site such as sake and shochu. It is preferable to do. As a handy type Raman spectroscope, a handy Raman spectroscope (Thirstec Indicator) sold by ST Japan Co., Ltd. is preferably exemplified.

  Laser light is used as the excitation light of the Raman spectroscopic device. The laser beam is preferably a laser beam in the near-infrared region, for example, a 785 nm laser beam. For example, laser light of 532 nm may be used as excitation light, but in this case, measurement sensitivity is reduced due to noise due to fluorescence.

  On the other hand, in the case of a handy Raman spectroscopy apparatus using excitation light having a relatively long wavelength such as 785 nm, it is not disturbed by fluorescence.

Ethanol quantification can be performed using a scattering peak around 872 cm ⁻¹ , characteristic of alcohol, and glucose quantification can use scattering peaks around 594 and 1120 cm ⁻¹ , characteristic of glucose. it can. Based on the intensity of the scattering peak peculiar to these alcohols and glucose, the ratios of ethanol and glucose can be set in a wide concentration range (ethanol 0.1 to 100%, glucose 0.1 to 30 by the univariate analysis / multivariate analysis). %) And can be measured accurately and simultaneously with good reproducibility. The sample amount required for measurement is sufficient if it is 1 mL, and even about 0.75 mL can be measured sufficiently. Although other components such as starch coexist in moromi, their presence does not adversely affect the determination of ethanol and glucose.

The size of the cell used for Raman scattering measurement should be as thin and small as possible in order to minimize the required measurement target sample. However, if the cell is too thin or too small, the intensity of the Raman scattering signal decreases, and sensitivity and accuracy are reduced. It is not preferable because it takes a long time to reduce the measurement and the measurement. The cell thickness is preferably 0.5 to 10 mm and the light receiving surface size is preferably about 10 to 400 mm ² , and the cell thickness is preferably 1 to 2 mm and the light receiving surface size is more preferably about 25 to 100 mm ² .

The cell material is preferably made of glass or transparent plastic having a transmittance of 90% or more with respect to light having a wavelength of 750 to 1000 nm.
Correlation analysis methods include ratioometry, univariate analysis / multivariate analysis, and the correlation equation depends on the installation position and cell size of the sample to be measured and the standard sample, but is linear, exponential, polynomial, logarithmic, power An approximation method or the like can be applied.
The filtration instrument of the present invention includes a pressure load container for extruding the mash while applying pressure from one side, a first filter unit, and a second filter unit (FIG. 5). In FIG. 5, the pressure load container is a syringe, and includes a syringe and a plunger.

  The first filter unit includes a first opening through which the crumb loaded with pressure flows, a region for mounting the first filter for filtering the mash, and the first filter. It has the 2nd opening part which sends out the filtered liquid substance, and the attachment part for mounting | wearing with or replacing the said 1st filter. The first opening is an upper opening in FIG. 5, and is connected to the pressure load container by a joint. The second opening is a lower opening in FIG. 5 and is connected to the second filter unit by a joint. In FIG. 5, the first filter is a filter paper and is attached to the region. The first filter can be mounted or replaced with an attachment such as a Seal ring as shown in FIG. When filter paper is used as the first filter, it is desirable to change the filter paper every time it is filtered. If the filter paper is replaced, the first filter unit can be used repeatedly.

  The second filter unit is a first opening through which the liquid material fed from the second opening of the first filter unit flows, and further for filtering the liquid material. And a second region through which a liquid component filtered by the second filter is sent out, and a second filter having a smaller pore diameter than the first filter. The first opening is an upper opening in FIG. 5 and is connected to the first filter unit by a joint. The second opening is a lower opening in FIG. 5 and is connected to a quartz cell or a glass cell by a joint. In FIG. 5, the second filter may be a finer filter than the first filter, and finer filter paper may be used, but a 0.45 μm membrane filter is more preferred. When a membrane filter is used as the second filter, it can be used a plurality of times (for example, 2 to 4 times) without replacement. An attachment part for mounting or replacing the second filter on the second filter unit may be further provided, and the second filter may be replaced.

  According to the present invention, it is possible to provide a measurement method that is dramatically lighter and smaller than the conventional method, excellent in portability, economically advantageous, and remarkably superior in terms of performance.

  Hereinafter, the present invention will be described in more detail with reference to Reference Examples, Examples and Test Examples, but these do not limit the present invention.

In addition, as a handy type Raman spectroscope, a Thurstec indicator sold by ST Japan Co., Ltd. was used, and measurement was performed at an exposure time (10.0 sec, 60.0 sec) and an excitation wavelength of 785 nm. It was.
Example 1
1. Experiment 1.1) Preparation of sake moromi sample and standard sample
About 10 mL of three kinds of sake moromi (provided by sake maker, No. 2, sake mash preparation No. 8, and sake mash preparation No. 18) provided by a Japanese sake maker are collected in a glass syringe, and 0.45 μm pores are collected. A colorless transparent sample was prepared by filtration using a filter having a diameter of 25 mm. Moromi No. 2, No. 8, and No. 18 are moromi at the end of the brewing stage, the middle stage, and the initial stage, respectively, and the alcohol concentrations analyzed using a gas chromatograph are 20.1 vol / vol%, 16. 9 vol / vol% and 5.1 vol / vol%. On the other hand, an aqueous solution containing 5-30 vol / vol% ethanol (Wako Pure Chemical Industries) was prepared as a sample for calibration. Similarly, an aqueous solution containing 1 to 300 g / L (0.1 to 10 wt%) of glucose (Wako Pure Chemical Industries) was prepared.

1.2) Raman scattering spectrum measurement of sake mash sample About 4 mL of sake mash sample that has not been filtered is added to a Pyrex (registered trademark) glass Bayer bottle, and a portable Raman spectrometer (ST Japan) A Raman scattering spectrum was measured in the backscattering mode. The excitation laser wavelength was 785 nm, and the measurement time was set to 10 seconds or 60 seconds. The measurement range was 400 cm ^{−1 to} 1400 cm ⁻¹ .

FIG. 1 shows Raman scattering spectra of three types of mashes that were not subjected to the filtration treatment, ethanol 20 vol / vol% aqueous solution, and glucose 2.0 wt% aqueous solution at a measurement time of 10 seconds. The crumbly sample and the aqueous ethanol solution showed scattering peaks due to ethanol at 872 cm ⁻¹ , 1020 cm ⁻¹ , 1080 cm ⁻¹ , and 1450 cm ⁻¹ , but the linear intensity is clearly between the scattering intensity and the concentration. No correlation was observed, and quantification with the required minimum accuracy (about 0.5% for both ethanol and glucose) was difficult. For example, Moromi Sample No. 2 at the end of the brewing stage contains approximately 20 vol / vol% ethanol, but its scattering intensity was significantly lower than that of the 20 vol / vol% ethanol aqueous solution.

Then, when the influence of the pretreatment of the moromi sample by various methods was examined, it was found that quantitative measurement of ethanol became possible by filtering using a filter having 0.45 μm pores. FIG. 2 (a) shows Raman scattering spectra of three types of mash subjected to filtration treatment, ethanol 20 vol / vol% aqueous solution, and glucose 20 g / L (2.0 wt%) containing aqueous solution at a measurement time of 10 seconds. . Compared with the case where the filtration treatment was not performed, the scattering intensity of the mash sample was increased, and a scattering peak due to glucose was observed in the vicinity of 1120 cm ⁻¹ . FIG. 2 (b) shows the results of plotting the scattering intensity at 872 cm ⁻¹ of the mash sample subjected to the filtration treatment, purified water, and an aqueous ethanol solution having a known concentration against the ethanol concentration. The coefficient of determination R ² is 0.9983, observed very good linear relationship was shown to be capable of quantification of almost required accuracy.

In order to evaluate the calibration limit and accuracy of glucose when ethanol and glucose coexist, Raman scattering measurement was performed for an aqueous solution containing 10 vol / vol% ethanol and 1 to 100 g / L glucose at a measurement time of 60 seconds. The results are shown in FIG. 3 (a) (the vertical axis indicates the numerical value converted to absorbance). The intensity of the scattering peak due to ethanol at 872 cm ⁻¹ is saturated and does not show a quantitative change, while the intensity of the other scattering peaks increases, and the scattering peak due to glucose is clearly around 1120 cm ^−1. Admitted. FIG. 3B shows the result of plotting the scattering intensity at 1120 cm ⁻¹ against the glucose concentration. The coefficient of determination ^{R 2} is 0.9956, observed very good linear relationship was found to be confirmed the presence or absence of 0.1% or more glucose. In addition, due to the fact that the absorption peak at 1080 cm ⁻¹ due to ethanol extends to this wavelength region, a constant scattering intensity was shown even when glucose was not contained.

By univariate and multivariate analysis of the above results, simultaneous determination of ethanol and glucose is possible. The mash sample subjected to the filtration treatment was subjected to univariate analysis based on the scattering peak intensities of 872 cm ⁻¹ and 1120 cm ⁻¹ , and moromi 2 had an alcohol concentration of 20.4 vol / vol% and a glucose concentration of 7. 2 wt%, Moromi No. 8 has an alcohol concentration of 16.9 vol / vol%, glucose concentration of 9.8 wt%, Moromi No. 18 has an alcohol concentration of 5.1 vol / vol% and a glucose concentration of 25.6 wt%. The measurement result by the method almost completely coincided (± 0.2% or less).

1.3) Examination of filtration method In the case of sake mash, filtration with a filter having pores of 0.45 μm was necessary. However, it takes 30 minutes or more to complete the 10 mL filtration. Therefore, for commercial makgeolli (both 375 mL bottles), the effect of filters with various pore diameters was examined, and the speeding up of processing by multistage filtration was examined. In FIG. 4, in addition to the Teflon (registered trademark) (PTFE) filter, it affects the Raman scattering spectrum of Macgoli at a measurement time of 10 seconds, and is filtered by a filter paper (No. 2), a glass filter (G3), a triangular width, gauze, etc. Shows the influence on the scattering intensity at 872 cm ⁻¹ . It should be noted that no clear scattering peak was observed in this region without filtration treatment. As a result, it was possible to quantify with good reproducibility by filtration with a filter having 0.45 μm pores, and no significant change was observed even when filtration was performed with a filter having 0.20 μm pores. On the other hand, quantitative analysis was impossible by filtration with filter paper.

  On the other hand, by using the instrument shown in FIG. 5 and performing two-stage filtration with a 0.45 μm PTFE filter after filtration with filter paper, the filtration can be completed efficiently and quickly. For example, for a laurel wreath (300 mL bottle), when a 0.45 μm PTFE filter with a diameter of 32 mm is attached to a glass syringe with a diameter of 9.2 mm, the initial filtration to less than 0.5 mL is a pressure of about 5 kg. However, after that, the force required for filtration increased rapidly to 10 kg or more, and filtration became very difficult. This is probably because solid components derived from garlic liquor accumulated on the filter. On the other hand, No. 1 having a diameter of 25 mm (effective diameter of 21 mm) shown in FIG. When a jig combining a 5A filter paper and a 0.45 μm PTFE filter is used, it can be rapidly filtered within 1 minute with a load of 5 kg or less up to the initial 1.0 mL. Furthermore, since the filter paper can be easily replaced, it is possible to select an optimal filter paper according to the amount of solid components in the sample and the particle diameter. Furthermore, by replacing only the filter paper, the PTFE syringe filter is repeatedly used about 2 to 4 times, and the cost of consumables required for filtration can be greatly reduced.

FIG. 6 shows a sample obtained by filtering laurel wreath (300 mL bottle, ethanol 10.5%) only with a 0.45 μm PTFE filter, A sample obtained by performing two-stage filtration with a 5A filter paper and a 0.45 μm PTFE filter, and a Raman scattering spectrum when nigari liquor was directly measured were shown. In the absence of filtration, almost no peaks due to ethanol at 872 cm ^-1 were observed, but in the case of one-stage and two-stage filtration, clear peaks of equal intensity were confirmed, and the effectiveness of two-stage filtration was demonstrated. It was done.

In addition, although the example which used the two-stage filtration system and the 0.45 micrometer filter was shown in the filtration method of a present Example, after filtering with a filter paper, and filtering with a 5-micrometer filter, it filters with a 0.45-micrometer filter, and further three-stage filtration But it has been confirmed that good results are obtained.
Moreover, although the present Example demonstrated the case of the filtration instrument shown in FIG. 5, this invention is not limited to this. For example, in the present embodiment, a glass syringe having a diameter of 9.2 mm was used, but any structure may be used as long as pressure can be applied to push the scum into the first filter unit. Furthermore, as the filters arranged in the first filter unit and the second filter unit, the pore size of the filter of the second filter unit is made smaller than the pore size of the filter of the first filter unit, so that it can be made in a short time. A filtrate capable of efficient and highly accurate quantitative measurement can be obtained.

  INDUSTRIAL APPLICABILITY The present invention can be used for manufacturing process management of a product containing brewed liquor / distilled liquor such as sake and shochu, alcohol, etc., or inspection of a product.

Claims (9)

Filtering the mash with a filter paper and a filter having a pore size of 1 μm or less, and filtering the solid components in the mash, and simultaneously quantifying the ethanol concentration and glucose concentration in the obtained filtrate with a Raman spectroscopic device, Simultaneous determination of ethanol concentration and glucose concentration in moromi. The method for simultaneously quantifying ethanol concentration and glucose concentration in mash according to claim 1, further comprising a step of filtering the mash with filter paper and further performing two-stage filtration using a filter having a pore size of 1 µm or less. The method for simultaneous determination of ethanol concentration and glucose concentration in mash according to claim 1 or 2, wherein the filter is a filter having a pore size of 0.45 µm or less. The method for simultaneous determination of ethanol concentration and glucose concentration in mash according to any one of claims 1 to 3, wherein the excitation light of the Raman spectroscopic device is excitation light in the near infrared region. The method for simultaneous determination of ethanol concentration and glucose concentration in a mash according to claim 4, wherein the excitation light is 785 nm laser light. The method for simultaneous determination of ethanol concentration and glucose concentration in mash according to any one of claims 1 to 5, wherein the Raman spectroscopic device is a portable device. The method for simultaneous determination of ethanol concentration and glucose concentration in mash according to any one of claims 1 to 6, wherein the mash is mash of sake, shochu, awamori or makgeolli. A filtration device for quantifying the concentration of ethanol and glucose in moromi,
A pressure load container for extruding the mash while applying pressure from one side;
A first opening for the flow of the moromi loaded with pressure, a region for mounting the first filter for filtering the mash, and a liquid material filtered by the first filter are sent out. A first filter unit having a second opening and an attachment for mounting or replacing the first filter;
A first opening through which the liquid material fed from the second opening of the first filter unit flows, and further for filtering the liquid material, wherein the first filter And a second filter unit having a second filter unit having a region where a second filter having a smaller pore diameter is mounted and a second opening for delivering a liquid component filtered by the second filter. Filtration instrument. The method for simultaneous determination of ethanol concentration and glucose concentration in mash according to claim 1, comprising a step of filtering solid components in the mash by two-stage filtration using the filtration device according to claim 8.