JP6598450B2 - Gene detection method for skin property determination - Google Patents
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Description
本発明は、皮膚性状判定のための遺伝子検出方法に関する。 The present invention relates to a gene detection method for skin property determination.
個々のヒトにおける、皮膚の粘弾性が強い、皮膚色が明るい、紫外線に対する感受性が高い、シワが少ない、角層水分量が多い、皮脂分泌量が多い、バリア機能が高い等の皮膚性状を知ることは、健康状態の把握だけでなく、化粧料の選択、化粧方法の選択にとっても重要である。従来、これらの皮膚性状は、年齢、環境、食事等により変化するものと考えられており、個々の皮膚性状を実際に測定し、それに対応する処置や化粧が行なわれてきた。例えば、角層水分量や皮脂分泌量を測定し、その量によって化粧方法を決定することなどが行なわれてきた。 To know skin properties such as strong skin viscoelasticity, bright skin color, high sensitivity to UV rays, low wrinkles, high stratum corneum moisture, high sebum secretion, high barrier function, etc. This is important not only for grasping the health condition, but also for selecting a cosmetic and a makeup method. Conventionally, these skin properties are considered to change depending on age, environment, meal, etc., and individual skin properties are actually measured, and corresponding treatments and makeup are performed. For example, the stratum corneum moisture content and sebum secretion amount are measured, and the makeup method is determined based on the amount.
一方、ミトコンドリアＤＮＡの遺伝子多型のバリエーションを指標として、民族の分類、各種疾患との関連性が指摘されている。例えば、脳梗塞、心筋梗塞、２型糖尿病、メタボリック症候群等の危険性がミトコンドリアハプログループの検出により判定する方法が報告されている（特許文献１〜４等）。 On the other hand, it has been pointed out that the mitochondrial DNA gene polymorphism variation is used as an index, and that it relates to ethnic classification and various diseases. For example, methods for determining the risk of cerebral infarction, myocardial infarction, type 2 diabetes, metabolic syndrome, etc. by detection of a mitochondrial haplogroup have been reported (Patent Documents 1 to 4, etc.).
皮膚性状に関する多くの項目をそれぞれ測定するのは煩雑であり、測定技術によって判定が困難であることから、個々の肌質（皮膚性状の一定の傾向）が一つの測定手段により判定できることが望ましい。
従って、本発明の課題は、皮膚性状の傾向を一つの手段により判定する方法を提供することにある。
Since it is cumbersome to measure many items related to skin properties, and it is difficult to determine by measurement techniques, it is desirable that individual skin quality (a certain tendency of skin properties) can be determined by one measuring means.
Therefore, an object of the present invention is to provide a method for determining a tendency of skin properties by one means.
そこで本発明者は、皮膚性状の傾向の判定手段につき種々検討し、９６２名の日本人女性のミトコンドリアＤＮＡのハプログループと皮膚性状との相関性を検討したところ、当該ハプログループを検出することにより、皮膚性状を判定できることを見出し、本発明を完成した。 Therefore, the present inventor examined various means for determining the tendency of the skin property and examined the correlation between the haplogroup of mitochondrial DNA and the skin property of 962 Japanese women. By detecting the haplogroup, The present inventors have found that skin properties can be determined and completed the present invention.
すなわち、本発明は、被験者の皮膚性状を判定する目的で、当該被験者のミトコンドリアＤＮＡのハプログループを検出することを特徴とする遺伝子検出方法を提供するものである。 That is, the present invention provides a gene detection method characterized by detecting a haplogroup of mitochondrial DNA of a subject for the purpose of determining the skin property of the subject.
本発明によれば、ミトコンドリアＤＮＡのハプログループの検出により、個々のヒトの各種皮膚性状の傾向が予想できるため、皮膚の管理方法、例えば化粧料の選択等が容易になる。 According to the present invention, by detecting the haplogroup of mitochondrial DNA, the tendency of various human skin properties can be predicted, so that a skin management method, for example, selection of cosmetics, etc. is facilitated.
本発明は、被験者の皮膚性状を判定する目的で、当該被験者のミトコンドリアＤＮＡのハプログループを検出することを特徴とする。 The present invention is characterized by detecting a haplogroup of mitochondrial DNA of a subject for the purpose of determining the skin property of the subject.
被験者としては、女性が好ましく、特に日本人女性が好ましい。 As a subject, a woman is preferable, and a Japanese woman is particularly preferable.
ミトコンドリアＤＮＡのハプログループの検出方法としては、従来公知の方法、例えばＤＮＡシークエンス、ＤＮＡチップ、ＤＮＡマイクロアレイ、ＲＦＬＰ、ＰＣＲ−ＳＳＯＰ−Ｌｕｍｉｎｅｘ法を用いることができるが、ＰＣＲ−ＳＳＯＰ−Ｌｕｍｉｎｅｘ法を用いるのが好ましい。ＰＣＲ−ＳＳＯＰ−Ｌｕｍｉｎｅｘ法は、前記の特許文献１〜４及びＩｍｍｕｎｏｇｅｎｅｔｉｃｓ（２００５）５７：７１７−７２９に記載されている。ハプログループ検出のためのミトコンドリアＤＮＡサンプルは、例えば血餅ＤＮＡを用いることができる。 As a method for detecting a haplogroup of mitochondrial DNA, a conventionally known method such as DNA sequence, DNA chip, DNA microarray, RFLP, PCR-SSOP-Luminex method can be used, but PCR-SSOP-Luminex method is used. Is preferred. The PCR-SSOP-Luminex method is described in the aforementioned Patent Documents 1 to 4 and Immunogenetics (2005) 57: 717-729. As a mitochondrial DNA sample for haplogroup detection, for example, clot DNA can be used.
ミトコンドリアＤＮＡのハプログループは、日本人の場合、Ａ、Ｂ、Ｄ４、Ｄ５、Ｆ、Ｇ１、Ｇ２、Ｍ７ａ、Ｍ７ｂ、Ｍ７ｃ、Ｎ９ａ、及びＮ９ｂの１２グループに分類されている（特許文献１〜４等）。当該各ハプログループ特有の遺伝子多型も既に知られている（特許文献１〜４等）。 The haplogroup of mitochondrial DNA is classified into 12 groups of A, B, D4, D5, F, G1, G2, M7a, M7b, M7c, N9a, and N9b in Japanese (Patent Documents 1 to 4). etc). The gene polymorphism peculiar to each haplo group is already known (Patent Documents 1 to 4, etc.)
本発明においては、日本人女性９６２名のミトコンドリアＤＮＡのハプログループと、皮膚性状との相関性を検討した。ミトコンドリアＤＮＡのハプログループＡ、Ｂ、Ｄ４、Ｄ５、Ｆ、Ｇ、Ｍ７ａ、Ｍ７ｂ、Ｎ９ａ及びＮ９ｂから選ばれる１０のグループと皮膚性状との間に相関性があることが認められた。 In the present invention, the correlation between the haplogroup of mitochondrial DNA of 962 Japanese women and the skin properties was examined. It was found that there was a correlation between skin properties and 10 groups selected from haplogroups A, B, D4, D5, F, G, M7a, M7b, N9a and N9b of mitochondrial DNA.
皮膚性状としては、皮膚粘弾性、皮膚色、紫外線感受性、シワ、角層水分量、皮脂分泌量及びバリア機能から選ばれる１種又は２種以上が挙げられる。皮膚粘弾性については、キュートメーター（Ｃｏｕｒａｇｅ ＋ Ｋｈａｚａｋａ社製）を用いて皮膚の粘弾性を測定し、Ｒ５（ハリ）、Ｒ６（たるみ）、Ｒ７（ハリ）を求めた。皮膚色については、分光測色計（ＣＭ−２６００ｄ、コニカミノルタセンシング(株)製）を用いて皮膚色（明るさ、赤味、黄味、）を測定した。また、皮膚色については、メラニン量指数、ヘモグロビン量指数も測定した。紫外線感受性については、皮膚に紫外線を照射し、翌日ＭＥＤ（肉眼的にもっとも軽い紅斑が出現するのに必要な紫外線エネルギー量：１ＭＥＤ＝６０〜８０ｍＪ／ｃｍ2）を判定した。シワについては、白色シルフロレプリカ剤（(株)アミックグループ製）を用いて目尻部のレプリカを採取し、非接触３Ｄ測定装置 ＰＲＩＭＯＳ ＰＩＣＯ（ＧＦＭｅｓｓｔｅｃｈｎｉｋ社）を用いてシワパラメータ（最大シワ深さ(Ｒｚ)、最大シワ最大深さ（Ｒｍａｘ）、総シワ平均深さ（Ｒａ））を測定した。角層水分量については、コルネオメーター（Ｃｏｕｒａｇｅ ＋ Ｋｈａｚａｋａ社製）を用いて角層水分量を測定した。皮脂分泌量については、セブメーター（Ｃｏｕｒａｇｅ ＋ Ｋｈａｚａｋａ社製）を用いて測定した。バリア機能については、ＴＥＷＡメーター（Ｃｏｕｒａｇｅ ＋ Ｋｈａｚａｋａ社製）を用いて経皮水分蒸散量（ＴＥＷＬ）を測定した。 Examples of skin properties include one or more selected from skin viscoelasticity, skin color, UV sensitivity, wrinkles, stratum corneum moisture, sebum secretion, and barrier function. Regarding skin viscoelasticity, the viscoelasticity of the skin was measured using a cutometer (Courage + Khazaka), and R5 (harness), R6 (sag), and R7 (harness) were obtained. About skin color, the skin color (brightness, redness, yellowness) was measured using the spectrophotometer (CM-2600d, Konica Minolta Sensing Co., Ltd. product). For skin color, the melanin index and hemoglobin index were also measured. For ultraviolet sensitivity, the skin was irradiated with ultraviolet rays, and the next day MED (the amount of ultraviolet energy required for the appearance of the lightest erythema to the naked eye: 1 MED = 60 to 80 mJ / cm 2 ) was determined. For wrinkles, a replica of the corner of the eye was collected using a white silflo replica agent (manufactured by Amic Group), and wrinkle parameters (maximum wrinkle depth (maximum wrinkle depth) (non-contact 3D measuring device PRIMOS PICO (GFMestechnik)) Rz), maximum wrinkle maximum depth (Rmax), and total wrinkle average depth (Ra)). Regarding the stratum corneum moisture content, the stratum corneum moisture content was measured using a Corneometer (Courage + Kazaka). The amount of sebum secretion was measured using a cebumeter (Courage + Kazaka). As for the barrier function, the transdermal water evaporation (TEWL) was measured using a TEWA meter (Courage + Khazaka).
その結果、ミトコンドリアＤＮＡのハプログループと皮膚性状との間には、相関性が認められ、具体的には、ハプログループの検出により以下の皮膚性状が判定できる。
（１）ハプログループＡのヒトは上腕のハリが弱く、頬のたるみが多く、上腕及び頬の皮膚色が明るく、紫外線感受性が高く、シワが少なく、角層水分量が少ないと判定する。
（２）ハプログループＢのヒトは皮脂分泌量が少なく、頬の皮膚色の赤味が強く、頬のハリが弱く、シワが多いと判定する。
（３）ハプログループＤ４のヒトは上腕及び頬の皮膚色が暗く、赤味が少なく、紫外線感受性が低く、バリア機能が高いと判定する。
（４）ハプログループＤ５のヒトは上腕のハリが強く、上腕の皮膚色が暗いと判定する。
（５）ハプログループＦのヒトは上腕の皮膚のヘモグロビン量が少ないと判定する。
（６）ハプログループＧのヒトは上腕のたるみが多く、頬のたるみが少なく、ハリが強く、上腕の皮膚色が黄味が強いと判定する。
（７）ハプログループＭ７ａのヒトは頬の皮膚色の赤味が強く、メラニン量が多く、上腕のヘモグロビン量が多く、頬の皮膚色が暗く、頬のたるみが多いと判定する。
（８）ハプログループＭ７ｂのヒトは上腕の皮膚色の黄味が強く、皮脂分泌量が多く、バリア機能が低いと判定する。
（９）ハプログループＮ９ａのヒトは上腕の皮膚色が暗いと判定する。
（10）ハプログループＮ９ｂのヒトは上腕及び頬のヘモグロビン量が少なく、上腕の皮膚色が明るく、頬の皮膚色が明るく、赤味が弱く、上腕のたるみが多く、紫外線感受性が低いと判定する。
As a result, a correlation is recognized between the haplogroup of mitochondrial DNA and the skin properties. Specifically, the following skin properties can be determined by detecting the haplogroup.
(1) Humans of haplogroup A are judged to have weak upper arm, large cheek sagging, bright upper arm and cheek skin color, high UV sensitivity, less wrinkle, and less stratum corneum moisture.
(2) Humans of haplogroup B are judged to have a low sebum secretion amount, a strong reddish cheek skin color, weak cheek tension, and a lot of wrinkles.
(3) Humans of haplogroup D4 are judged to have dark upper arm and cheek skin color, less redness, low UV sensitivity, and high barrier function.
(4) The human of the haplogroup D5 is determined to have strong upper arm tension and dark skin color of the upper arm.
(5) A haplogroup F human is determined to have a low amount of hemoglobin in the upper arm skin.
(6) It is determined that the humans of the haplogroup G have a lot of upper arm sagging, little cheek sagging, firmness, and upper arm skin color is strongly yellowish.
(7) Humans of the haplogroup M7a are judged to have a strong reddish cheek skin color, a large amount of melanin, a large amount of hemoglobin in the upper arm, a dark skin color on the cheek, and a large amount of sagging on the cheek.
(8) Humans of the haplogroup M7b are judged to have a strong yellowish skin color of the upper arm, a large amount of sebum secretion, and a low barrier function.
(9) The human of the haplogroup N9a determines that the upper arm skin color is dark.
(10) Humans of haplogroup N9b are judged to have less hemoglobin in the upper arm and cheek, brighter upper skin color, brighter cheek skin color, less reddish, more upper arm sagging, and low UV sensitivity .
本発明方法によれば、ミトコンドリアＤＮＡのハプログループ検出を行えば、そのヒトの皮膚性状の傾向が予想できるため、皮膚性状の管理、化粧方法等が決定できる。 According to the method of the present invention, if the haplogroup detection of mitochondrial DNA is performed, the tendency of the human skin property can be predicted, so the management of the skin property, the cosmetic method, etc. can be determined.
上述した実施形態に関し、本発明はさらに以下の方法を開示する。 In relation to the above-described embodiment, the present invention further discloses the following method.
＜１＞被験者の皮膚性状を判定する目的で、当該被験者のミトコンドリアＤＮＡのハプログループを検出することを特徴とする遺伝子検出方法。 <1> A gene detection method comprising detecting a haplogroup of mitochondrial DNA of a subject for the purpose of determining the subject's skin properties.
＜２＞皮膚性状が、皮膚粘弾性、皮膚色、紫外線感受性、シワ、角層水分量、皮脂分泌量及びバリア機能から選ばれる１種又は２種以上である＜１＞記載の遺伝子検出方法。
＜３＞ミトコンドリアＤＮＡのハプログループが、Ａ、Ｂ、Ｄ４、Ｄ５、Ｆ、Ｇ、Ｍ７ａ、Ｍ７ｂ、Ｎ９ａ及びＮ９ｂから選ばれるグループである＜１＞又は＜２＞記載の遺伝子検出方法。
＜４＞ハプログループＡのヒトは上腕のハリが弱く、頬のたるみが多く、上腕及び頬の皮膚色が明るく、紫外線感受性が高く、シワが少なく、角層水分量が少ないと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜５＞ハプログループＢのヒトは皮脂分泌量が少なく、頬の皮膚色の赤味が強く、頬のハリが弱く、シワが多いと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜６＞ハプログループＤ４のヒトは上腕及び頬の皮膚色が暗く、赤味が少なく、紫外線感受性が低く、バリア機能が高いと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜７＞ハプログループＤ５のヒトは上腕のハリが強く、上腕の皮膚色が暗いと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜８＞ハプログループＦのヒトは上腕の皮膚のヘモグロビン量が少ないと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜９＞ハプログループＧのヒトは上腕のたるみが多く、頬のたるみが少なく、ハリが強く、上腕の皮膚色が黄味が強いと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜10＞ハプログループＭ７ａのヒトは頬の皮膚色の赤味が強く、メラニン量が多く、上腕のヘモグロビン量が多く、頬の皮膚色が暗く、頬のたるみが多いと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜11＞ハプログループＭ７ｂのヒトは上腕の皮膚色の黄味が強く、皮脂分泌量が多く、バリア機能が低いと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜12＞ハプログループＮ９ａのヒトは上腕の皮膚色が暗いと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜13＞ハプログループＮ９ｂのヒトは上腕及び頬のヘモグロビン量が少なく、上腕の皮膚色が明るく、頬の皮膚色が明るく、赤味が弱く、上腕のたるみが多く、紫外線感受性が低いと判定する＜１＞〜＜３＞のいずれかに記載の遺伝子検出方法。
＜14＞被験者が、日本人女性である＜１＞〜＜１３＞のいずれかに記載の遺伝子検出方法。
<2> The gene detection method according to <1>, wherein the skin property is one or more selected from skin viscoelasticity, skin color, UV sensitivity, wrinkles, stratum corneum moisture content, sebum secretion amount, and barrier function.
<3> The gene detection method according to <1> or <2>, wherein the haplogroup of mitochondrial DNA is a group selected from A, B, D4, D5, F, G, M7a, M7b, N9a, and N9b.
<4> Haplo group A humans are judged to have weak upper arms, large cheek sagging, bright upper arm and cheek skin color, high UV sensitivity, low wrinkles, and low stratum corneum moisture <1 > The gene detection method according to any one of <3>.
<5> The haplogroup B human has a low sebum secretion amount, a strong redness of the cheek skin color, a weak cheek tension, and a lot of wrinkles. <1> to <3> Gene detection method.
<6> The gene detection according to any one of <1> to <3>, wherein humans of haplogroup D4 are judged to have dark upper arm and cheek skin color, less redness, low UV sensitivity, and high barrier function Method.
<7> The gene detection method according to any one of <1> to <3>, wherein humans of haplogroup D5 are determined to have strong upper arm tension and dark upper arm skin color.
<8> The gene detection method according to any one of <1> to <3>, wherein a human of haplogroup F determines that the amount of hemoglobin in the upper arm skin is small.
<9> The human of the haplo group G has a lot of sagging of the upper arm, little cheek sagging, strong firmness, and the skin color of the upper arm is judged to be yellowish. <1> to <3> Gene detection method.
<10> Humans of haplogroup M7a are judged to have strong reddish cheek skin color, high melanin content, high hemoglobin content in the upper arm, dark cheek skin color, and high cheek sagging <1> to <3> The gene detection method according to any one of the above.
<11> The gene detection method according to any one of <1> to <3>, wherein humans of the haplogroup M7b are judged to have a strong yellowish skin color of the upper arm, a large amount of sebum secretion, and a low barrier function.
<12> The gene detection method according to any one of <1> to <3>, wherein the human of the haplogroup N9a determines that the skin color of the upper arm is dark.
<13> Haplo group N9b humans are judged to have low hemoglobin content in the upper arms and cheeks, lighter upper arm skin color, brighter cheek skin color, weak redness, more upper arm sagging, and lower UV sensitivity The gene detection method according to any one of <1> to <3>.
<14> The gene detection method according to any one of <1> to <13>, wherein the subject is a Japanese woman.
次に実施例を挙げて本発明を詳細に説明する。 EXAMPLES Next, an Example is given and this invention is demonstrated in detail.
実施例１（ミトコンドリアＤＮＡのハプログループの検出）
日本人女性９６２名から得られた血液から分離した血餅からＤＮＡを常法に従って抽出した。ＤＮＡ（１μｇ）を鋳型として使用し、遺伝子増幅する。ミトコンドリアＤＮＡ（ｍｔＤＮＡ）のコード領域をｍｕｌｔｉｐｌｅｘ ＰＣＲ法により増幅し、Ｌｕｍｉｎｅｘ１００システムを用いてミトコンドリアゲノム多型を分析する。これまでに、日本人６７２人のｍｔＤＮＡ全塩基配列の分析に基づいてミトコンドリアゲノムの分子系統樹を得ている（Ｔａｎａｋａ ｅｔ ａｌ．Ｇｅｎｏｍｅ Ｒｅｓ １４：１８３２−１８５０，２００４）。この系統樹に基づいて多型部位を選定、一斉分析によりミトコンドリアゲノムの型をＡ、Ｂ、Ｄ４、Ｄ５、Ｆ、Ｇ、Ｍ７ａ、Ｍ７ｂ、Ｎ９ａ、Ｎ９ｂのハプログループに分類する。
このミトコンドリアＤＮＡの遺伝子多型の分析及びハプログループの分類は、詳細には、特許文献１〜４の記載に従って行った。
Example 1 (Detection of haplogroup of mitochondrial DNA)
DNA was extracted from blood clots isolated from blood obtained from 962 Japanese women according to a conventional method. Gene amplification is performed using DNA (1 μg) as a template. The coding region of mitochondrial DNA (mtDNA) is amplified by the multiplex PCR method, and the mitochondrial genomic polymorphism is analyzed using the Luminex100 system. So far, a molecular phylogenetic tree of the mitochondrial genome has been obtained based on the analysis of the entire mtDNA base sequence of 672 Japanese (Tanaka et al. Genome Res 14: 1832-1850, 2004). Based on this phylogenetic tree, polymorphic sites are selected, and mitochondrial genome types are classified into haplogroups of A, B, D4, D5, F, G, M7a, M7b, N9a, and N9b by simultaneous analysis.
The analysis of gene polymorphism of mitochondrial DNA and the classification of haplogroups were carried out in detail according to the descriptions in Patent Documents 1 to 4.
実施例２（皮膚性状の計測）
実施例１でハプログループの分類を行った被験者に対し、（表１）の皮膚性状計測を行った。
Example 2 (Measurement of skin properties)
The skin property measurement of (Table 1) was performed on subjects who performed haplogroup classification in Example 1.
表１中の皮膚計測の詳細は以下の如く行った。 The details of the skin measurement in Table 1 were as follows.
（１）紫外線感受性（上腕）
被験者の片側上腕内側に、１ｍＷ／ｃｍ2の照射率で紫外線（ＵＶ）を照射する。この際に照射時間を７点振って異なるエネルギー量（７段階）の紫外線（ＵＶ）に暴露させる（０．５ｃｍ2×７ヶ所）。紫外線（ＵＶ）照射の翌日、研究者の目視により研究者のＭＥＤを判定した。
ここで、ＭＥＤには個人差があり、１ＭＥＤ＝６０〜８０ｍＪ／ｃｍ2であるとされている。
１ｍＷ・ｓｅｃ＝１ｍＪ。したがって、１ｍＷ／ｃｍ2の照射率で６０秒間照射した場合は、６０ｍＪ／ｃｍ2となる。
紫外線を皮膚に照射すると照射後２４時間をピークにして紅斑が出現するが、肉眼的にもっとも軽い紅斑が出現するのに必要なエネルギー量をＭＥＤという。
日本においては真夏の関東周辺の海岸で１ＭＥＤに要する暴露時間は２０分程度であり、１時間あたり３ＭＥＤを受光することになる。
個人差はあるが一般に、かゆみや軽い痛みを伴う日焼けでは４ＭＥＤ、水疱が出来るようなケースでは８ＭＥＤ以上を連続して受光したものと考えられている。
(1) UV sensitivity (upper arm)
The inside of the upper arm of one side of the subject is irradiated with ultraviolet rays (UV) at an irradiation rate of 1 mW / cm 2 . At this time, the irradiation time is changed by 7 points and exposed to ultraviolet rays (UV) having different energy amounts (7 levels) (0.5 cm 2 × 7 locations). The day after the ultraviolet (UV) irradiation, the researcher's MED was determined visually by the researcher.
Here, there are individual differences in MED, and 1 MED = 60 to 80 mJ / cm 2 .
1 mW · sec = 1 mJ. Therefore, when irradiation is performed at an irradiation rate of 1 mW / cm 2 for 60 seconds, it is 60 mJ / cm 2 .
When the skin is irradiated with ultraviolet rays, erythema appears at a peak 24 hours after irradiation, and the amount of energy required for the appearance of the lightest erythema to the naked eye is called MED.
In Japan, the exposure time required for 1 MED on the coast around Kanto in midsummer is about 20 minutes, and 3 MED is received per hour.
Although there are individual differences, in general, it is considered that 4 MED is continuously received in the case of sunburn accompanied by itching and mild pain, and 8 MED or more is continuously received in cases where blisters are formed.
（２）皮膚粘弾性（頬、上腕）
キュートメーター（Ｃｏｕｒａｇｅ ＋ Ｋｈａｚａｋａ社製）を用いて片側頬部、片側上腕内側部の弾力性を計測した。計測値（Ｒ５、Ｒ６、Ｒ７）を取得した。
(2) Skin viscoelasticity (cheek, upper arm)
The elasticity of one side cheek and one side upper arm was measured using a cutometer (Courage + Khazaka). Measurement values (R5, R6, R7) were obtained.
（３）皮膚色（頬、上腕）
分光測色計（ＣＭ−２６００ｄ、コニカミノルタセンシング株式会社製）を用いて頬部、上腕内側部の肌色を計測した。
明るさ（Ｌ＊値）、赤味（ａ＊値）、黄味（ｂ＊値）、メラニン量指数、ヘモグロビン量指数を取得した。
(3) Skin color (cheek, upper arm)
Using a spectrocolorimeter (CM-2600d, manufactured by Konica Minolta Sensing Co., Ltd.), the skin color of the cheek and the inner side of the upper arm was measured.
Brightness (L * value), redness (a * value), yellowness (b * value), melanin index, and hemoglobin index were obtained.
（４）シワ（目尻）
白色シルフロレプリカ剤（株式会社アミックグループ製）を用いて片側目尻部のレプリカを採取した。非接触３Ｄ測定装置ＰＲＩＭＯＳ ＰＩＣＯ（ＧＦＭｅｓｓｔｅｃｈｎｉｋ社）を用いてシワに関するパラメーター（＊）を測定した。
シワパラメーターは、最大シワ平均深さ（Ｒｚ）、最大シワ最大深さ（Ｒｍａｘ）、総シワ平均深さ（Ｒａ）である。
(4) Wrinkles
A replica of one eye corner was collected using a white Sylphlo replica agent (manufactured by Amic Group). A wrinkle parameter (*) was measured using a non-contact 3D measuring device PRIMOS PICO (GFMestechnik).
The wrinkle parameters are maximum wrinkle average depth (Rz), maximum wrinkle maximum depth (Rmax), and total wrinkle average depth (Ra).
（５）角層水分量（上腕）
コルネオメーター（Ｃｏｕｒａｇｅ ＋ Ｋｈａｚａｋａ社製）を用いて片側上腕内側部の水分量を計測した。コルネオメーターは、皮膚表面から約１５μｍ（主に角層）に含まれる水分量を測定するとされている。
(5) stratum corneum moisture content (upper arm)
The water content of the inner part of one upper arm was measured using a Corneometer (Courage + Khazaka). The Corneometer is supposed to measure the amount of water contained in about 15 μm (mainly stratum corneum) from the skin surface.
（６）バリア機能（頬、上腕）
ＴＥＷＡメーター（Ｃｏｕｒａｇｅ ＋ Ｋｈａｚａｋａ社製）を用いて片側頬部、片側上腕内側部の経皮水分蒸散量（ＴＥＷＬ）を計測した。
（７）皮脂分泌量（額）
セブメーター（Ｃｏｕｒａｇｅ ＋ Ｋｈａｚａｋａ社製）を用いて額部の皮脂量（回復値）を計測した。
(6) Barrier function (cheek, upper arm)
Using a TEWA meter (Courage + Kazaka), the transdermal water transpiration (TEWL) of one side cheek and one side upper arm was measured.
(7) Sebum secretion (forehead)
The amount of sebum (recovery value) in the forehead was measured using a cebu meter (Courage + Khazaka).
実施例３（統計処理）
皮膚計測データとハプログループ間の相関性を解析した。解析にあたり、各計測項目について、計測値と人数構成をもとに中央値を算出後、低値群（Ｌｏｗｅｒ）と高値群（Ｈｉｇｈｅｒ）とに分類した。
各計測値の高低におけるｍｔハプログループの特徴について、Ｆｉｓｈｅｒの相関性係数、オッズ比と９５％信頼性区間を算出した。
解析には、ＪＭＰ（ｖｅｒ．９，ＳＡＳ ｉｎｔｅｒｎａｔｉｏｎａｌ）を使用した。
Ｆｉｓｈｅｒの相関性係数の算出値（Ｐ値）が０．１よりも小さかった場合に、その皮膚計測データとミトコンドリアハプログループとに関連性があると判断した。
Example 3 (statistical processing)
The correlation between skin measurement data and haplogroup was analyzed. In the analysis, each measurement item was classified into a low value group (Lower) and a high value group (Higher) after calculating the median based on the measurement value and the number of people.
Fisher's correlation coefficient, odds ratio and 95% confidence interval were calculated for the features of the mt haplogroup at each measurement level.
JMP (ver. 9, SAS international) was used for the analysis.
When the calculated value (P value) of Fisher's correlation coefficient was smaller than 0.1, it was determined that the skin measurement data and the mitochondrial haplogroup were related.
結果を表２〜表３に示す。 The results are shown in Tables 2 to 3.
表２〜表３より、ミトコンドリアハプログループの検出により、以下の皮膚性状が判定できることがわかった。 From Tables 2 to 3, it was found that the following skin properties can be determined by detection of the mitochondrial haplogroup.
（１）ハプログループＡのヒトは上腕のハリが弱く、頬のたるみが多く、上腕及び頬の皮膚色が明るく、紫外線感受性が高く、シワが少なく、角層水分量が少ないと判定できる。
（２）ハプログループＢのヒトは皮脂分泌量が少なく、頬の皮膚色の赤味が強く、頬のハリが弱く、シワが多いと判定できる。
（３）ハプログループＤ４のヒトは上腕及び頬の皮膚色が暗く、赤味が少なく、紫外線感受性が低く、バリア機能が高いと判定できる。
（４）ハプログループＤ５のヒトは上腕のハリが強く、上腕の皮膚色が暗いと判定できる。
（５）ハプログループＦのヒトは上腕の皮膚のヘモグロビン量が少ないと判定できる。
（６）ハプログループＧのヒトは上腕のたるみが多く、頬のたるみが少なく、ハリが強く、上腕の皮膚色が黄味が強いと判定できる。
（７）ハプログループＭ７ａのヒトは頬の皮膚色の赤味が強く、メラニン量が多く、上腕のヘモグロビン量が多く、頬の皮膚色が暗く、頬のたるみが多いと判定できる。
（８）ハプログループＭ７ｂのヒトは上腕の皮膚色の黄味が強く、皮脂分泌量が多く、バリア機能が低いと判定できる。
（９）ハプログループＮ９ａのヒトは上腕の皮膚色が暗いと判定できる。
（10）ハプログループＮ９ｂのヒトは上腕及び頬のヘモグロビン量が少なく、上腕の皮膚色が明るく、頬の皮膚色が明るく、赤味が弱く、上腕のたるみが多く、紫外線感受性が低いと判定できる。
(1) It can be determined that humans of haplogroup A have weak upper arm, much sagging cheeks, bright skin color of upper arm and cheeks, high UV sensitivity, less wrinkles, and less stratum corneum moisture.
(2) It can be determined that humans of haplogroup B have a small amount of sebum secretion, strong reddish cheek skin color, weak cheek tension, and many wrinkles.
(3) It can be determined that humans of the haplogroup D4 have dark upper arm and cheek skin colors, less redness, low UV sensitivity, and high barrier function.
(4) It can be determined that humans of the haplogroup D5 have strong upper arm tension and dark upper arm skin color.
(5) It can be determined that humans of haplogroup F have a small amount of hemoglobin in the upper arm skin.
(6) It can be determined that humans of the haplogroup G have much upper arm sagging, little cheek sagging, firmness, and upper arm skin color is strongly yellowish.
(7) It can be determined that the humans of the haplogroup M7a have a strong reddish skin color on the cheek, a large amount of melanin, a large amount of hemoglobin in the upper arm, a dark skin color on the cheek, and a lot of cheek sagging.
(8) It can be determined that humans of the haplogroup M7b have a strong yellowish skin color of the upper arm, a large amount of sebum secretion, and a low barrier function.
(9) It can be determined that humans of the haplogroup N9a have dark upper arm skin color.
(10) It can be determined that humans of haplogroup N9b have low amounts of hemoglobin on the upper arm and cheek, bright upper skin color, bright cheek skin color, weak redness, high upper arm sagging, and low UV sensitivity. .
Claims (1)
（１）ハプログループＡは上腕のハリが弱く、頬のたるみが多く、上腕及び頬の皮膚色が明るく、紫外線感受性が高く、シワが少なく、角層水分量が少ない、
（２）ハプログループＢは皮脂分泌量が少なく、頬の皮膚色の赤味が強く、頬のハリが弱く、シワが多い、
（３）ハプログループＤ４は上腕及び頬の皮膚色が暗く、赤味が少なく、紫外線感受性が低く、バリア機能が高い、
（４）ハプログループＤ５は上腕のハリが強く、上腕の皮膚色が暗い、
（５）ハプログループＦは上腕の皮膚のヘモグロビン量が少ない、
（６）ハプログループＧは上腕のたるみが多く、頬のたるみが少なく、ハリが強く、上腕の皮膚色が黄味が強い、
（７）ハプログループＭ７ａは頬の皮膚色の赤味が強く、メラニン量が多く、上腕のヘモグロビン量が多く、頬の皮膚色が暗く、頬のたるみが多い、
（８）ハプログループＭ７ｂは上腕の皮膚色の黄味が強く、皮脂分泌量が多く、バリア機能が低い、
（９）ハプログループＮ９ａは上腕の皮膚色が暗い、
（10）ハプログループＮ９ｂは上腕及び頬のヘモグロビン量が少なく、上腕の皮膚色が明るく、頬の皮膚色が明るく、赤味が弱く、上腕のたるみが多く、紫外線感受性が低い。 Detecting a haplogroup selected from A, B, D4, D5, F, G, M7a, M7b, N9a and N9b of mitochondrial DNA in a sample containing mitochondrial DNA derived from a Japanese female subject, A gene detection method relating to skin properties, wherein the gene detection method is classified into at least one of the following (1) to (10).
(1) Haplo group A has weak upper arm, large sagging cheeks, bright upper arm and cheek skin color, high UV sensitivity, less wrinkles, less stratum corneum moisture,
(2) Haplo group B has a small amount of sebum secretion, strong reddish cheek skin color, weak cheek tension, and lots of wrinkles,
(3) Haplo group D4 has dark upper arm and cheek skin color, less redness, low UV sensitivity, and high barrier function.
(4) Haplo group D5 has strong upper arm tension and dark upper arm skin color.
(5) Haplo group F has less hemoglobin in the upper arm skin,
(6) Haplo group G has a lot of upper arm sagging, little cheek sagging, firmness, and upper arm skin color is strongly yellowish.
(7) The haplogroup M7a has a strong reddish skin color on the cheeks, a large amount of melanin, a large amount of hemoglobin on the upper arm, a dark skin color on the cheeks, and a lot of sagging on the cheeks.
(8) The haplogroup M7b has a strong yellowish skin color of the upper arm, a large amount of sebum secretion, and a low barrier function.
(9) Haplo group N9a has dark upper arm skin color,
(10) The haplogroup N9b has a small amount of hemoglobin in the upper arm and cheek, has a bright upper arm skin color, a bright cheek skin color, weak redness, a large upper arm sagging, and low UV sensitivity.
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