JP6589037B1 - Sampling instrument and sampling method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a sampling device and a sampling method capable of stably collecting a predetermined amount of sample. A sample collecting device 1 is fixed to a main body 2, a main body 2 and a sampling part 3 capable of collecting a sample, and is detachably attached to the main body 2. 9 is mainly composed of a quantitative unit 4 that exposes a part of the sampling unit 3. By attaching or removing the quantification unit 4 to or from the main body unit 2, the sampling unit 3 passes through the through-hole 9 in a constant contact state, and the amount of the sample collected by the sampling unit 3 is adjusted. In this way, the amount of the sample remaining in the collection unit 3 is stabilized, so that the accuracy of the inspection using the sample is improved. [Selection] Figure 2

Description

  The present invention relates to a sample collection device and a sample collection method, and more particularly to a sample collection device and a sample collection method for collecting samples such as blood, saliva and other body fluids, tissues, plaques, and excreta.

  Conventionally, there are sampling devices for collecting samples such as blood, saliva and other body fluids, tissues, plaques, and excreta.

  For example, Patent Document 1 discloses a dropper-like sampling device that sucks or blows a sample. The subject to be used directly injects saliva as a sample into the saliva inlet of the sample collection device.

  Patent Document 2 discloses a pick-like sampling device that scrapes a sample. Using a minute flat plaque collecting part provided at the tip of the sample collecting device, plaque as a sample is collected from the tooth surface.

  Furthermore, Patent Document 3 discloses a spatula-like sampling device that scoops up a sample. Using the sampling piece provided on the stopper, the stool or the like as a sample is scooped up and collected.

JP 2006-275809 A JP 2006-198172 A JP 2018-072264 A

  However, any of the above-described conventional sample collection instruments can be performed until the amount of the collected sample is adjusted to a predetermined amount (hereinafter, the adjustment to the predetermined amount may be expressed as “quantitative”). It was difficult.

  The collected sample may be used as a specimen for PCR and other examinations and analyses. At that time, even if a sufficient amount was collected for the inspection, if the amount of the sample was too large, there was a risk of hindering the reaction related to the inspection.

  Although it is possible to measure a desired amount by using a laboratory instrument such as a micropipette, it is cumbersome to use such a laboratory instrument separately from the sampling instrument, and depending on the skill of the user. There was also a risk that the test results would change. Therefore, stable quantification of the collected sample has been awaited because it leads to an improvement in inspection accuracy.

  The present invention has been made to solve the above-described problems, and an object of the present invention is to provide a sampling device and a sampling method that can stably obtain a predetermined amount of a sample.

  In order to achieve the above-mentioned object, the invention according to claim 1 is characterized in that a main body part, a sampling part fixed to the main body part and capable of collecting a sample, and detachably attached to the main body part, A quantitative unit that exposes at least a part of the sampling part from the through-hole, and the quantitative part is a cap body that includes a through-hole at the tip and covers a part of the main body part in the attached state, , A shaft member extending in the first direction, and a bendable collecting member provided on at least a part of the shaft member for collecting a sample. Constructed in a rod shape, the sampling member includes a filament material that is fixed in a spiral gap between the wires and protrudes in a direction different from the first direction from the shaft material, and is attached or detached from the main body of the quantitative unit Sampling at the sampling section By member passes through the through hole, a sampling unit where the amount of sample taken in collecting portion is adjusted.

  If comprised in this way, the quantity of the sample remaining in a collection part will be stabilized. In addition, the quantitative unit can be attached and detached without requiring a complicated operation. Furthermore, sample collection and sample dispersion after quantification are facilitated.

  According to a second aspect of the present invention, in the configuration of the first aspect of the invention, the passage diameter of the through hole is set larger than the diameter of the shaft member and smaller than the diameter of the sampling member before bending, and the fixed portion is attached. Or the part exceeding the predetermined amount is removed among the extract | collected samples because the collection member in a collection part passes a through-hole by removal.

  If comprised in this way, the residual amount of the extract | collected sample can be designed by the setting of the passage diameter of a through-hole.

  According to a third aspect of the present invention, in the configuration of the second aspect of the invention, the fixed amount portion moves from the upstream side in the fixed direction to the downstream side in attachment or detachment, and the through hole is configured by an upstream opening edge. The surface area is larger than the area formed by the downstream opening edge.

If comprised in this way, fixed_quantity | quantitative_assay will advance gradually when a collection member is passed through a through-hole from the upstream.
According to a fourth aspect of the present invention, in the configuration of the first aspect of the present invention, the diameter of the sampling member before bending is 1.0 mm to 15.0 mm, and the minimum passage hole of the sampling portion The diameter is 0.4 mm to 2.5 mm, the passage diameter of the through hole of the fixed amount portion is 0.3 mm to 5.0 mm, and the passage diameter of the through hole is 10% with respect to the diameter of the sampling member before bending. ~ 80%.
If comprised in this way, in a fixed_quantity | assay process, a fixed_quantity | quantitative_assay part can be stably removed from a main-body part with a fixed contact state with respect to a collection part.

  The invention according to claim 5 is the configuration of the invention according to any one of claims 1 to 4, further comprising an overcap that encloses the sampling part and the quantitative part and fits outside the quantitative part. is there.

  If comprised in this way, a fixed_quantity | quantitative_assay part can be simultaneously removed by removing the overcap attached after extraction.

  The invention described in claim 6 includes a step of collecting a sample by a sampling part fixed to the main body part, and a fixed amount that is detachably attached to the main body part and exposes at least a part of the sampling part from the through hole in the attached state. And removing the part of the sample collected in the sampling part by attaching to or removing from the main part in a fixed contact state with the sampling part. And a sampling body that covers a part of the main body part in the attached state, the sampling part being provided on at least a part of the shaft member extending in the first direction and collecting the sample. The shaft member is configured in a rod shape as a whole shaft member by twisting the wire in a spiral shape, and the sampling member is fixed in the spiral gap between the wires, and the shaft member has a first direction from the shaft member. Is a filler that protrudes in different directions Containing cement material is a sampling method.

  With this configuration, a desired amount of sample can be obtained regardless of the amount collected. In addition, the quantitative unit can be attached and detached without requiring a complicated operation. Furthermore, sample collection and sample dispersion after quantification are facilitated.

  As described above, according to the first aspect of the present invention, since the amount of the sample remaining in the sampling part is stabilized, the accuracy of the inspection using the sample is improved. In addition, since the quantitative unit can be attached and detached without requiring a complicated operation, the simplicity of quantitative measurement is improved. Furthermore, since sample collection and sample dispersion after quantification are facilitated, sample collection efficiency and usability are improved.

  In addition to the effect of the invention described in claim 1, the invention described in claim 2 can design the residual amount of the collected sample by setting the passage diameter of the through-hole, so that the certainty of determination is improved. .

In addition to the effect of the invention described in claim 2, the invention described in claim 3 is improved in quantification because the quantification proceeds gradually when the sampling member is passed through the through-hole from the upstream side.
In addition to the effect of the invention according to any one of claims 1 to 3, the invention described in claim 4 removes the quantification part from the main body part in a stable and constant contact state with the sampling part in the quantification step. Therefore, the quantitative efficiency is good.

  In addition to the effect of the invention according to any one of claims 1 to 4, the invention according to claim 5 can remove the quantification unit at the same time by removing the overcap attached after collection. Can be removed without directly touching, improving the certainty of determination.

  According to the sixth aspect of the present invention, since a desired amount of sample can be obtained regardless of the amount to be collected, the accuracy of the inspection using the sample is improved. In addition, since the quantitative unit can be attached and detached without requiring a complicated operation, the simplicity of quantitative measurement is improved. Furthermore, since sample collection and sample dispersion after quantification are facilitated, sample collection efficiency and usability are improved.

It is a front view which shows the external appearance shape of the sample-collecting instrument by 1st Embodiment of this invention. It is the front view which fractured | ruptured the fixed_quantity | quantitative_assay part so that the center cross-sectional structure might be represented among the sampling devices shown in FIG. 1, (1) is the state before attachment, (2) is the state after attachment. . FIG. 3 is an enlarged view of the “A” portion shown in FIG. 2, where (1) is a state before the quantification process, (2) is an intermediate state of the quantification process, and (3) is a state after the quantification process. is there. It is the front view partly fractured | ruptured so that the central section structure of the fixed_quantity | quantitative_assay part and an overcap could be represented among the sampling devices by 2nd Embodiment of this invention. It is a front view of the sampling instrument shown in FIG. 4, Comprising: (1) is an attachment state, (2) is a removal state.

  FIG. 1 is a front view showing the external shape of a sampling device according to the first embodiment of the present invention, and FIG. 2 shows a central sectional structure of a quantitative portion of the sampling device shown in FIG. It is the front view which fractured | ruptured, Comprising: (1) is the state before attachment, (2) is the state after attachment.

  Referring to these drawings, a sample collection device 1 includes a polyethylene main body 2 gripped by a user, a collection unit 3 that is fixed to the tip of the main body 2 and can collect a sample, and a main body. 2 is mainly composed of a fixed portion 4 made of a polypropylene cap body detachably attached to 2.

The main body portion 2 and the sampling portion 3 constitute a so-called interdental brush-like shape. The sampling unit 3 includes a stainless steel shaft 6 extending in the same direction (longitudinal direction) as the main body 2 extends, and a sampling member 7 that is provided in a substantially entire peripheral region of the shaft 6 and collects a sample. It is mainly composed. The sampling member 7 includes a nylon filament material protruding radially from the shaft member 6 in the lateral direction, and can be bent so as to be deformed in the lateral direction by pressure from the longitudinal direction or the lateral direction. More specifically, referring to (1) of FIG. 3 described later, the shaft 6 has a diameter (D ₁ ) as a whole of the shaft 6 by twisting a wire (not shown) having a diameter of 0.2 mm spirally. It is configured in a rod shape of 0.3 mm, and a large number of filament materials having a diameter of 0.055 mm are fixed in the spiral gap between the wires, and the diameter before bending (D ₂ ) is 2.0 mm. Therefore, the minimum passing diameter of the sampling part 3 in a state where the filament material is bent at the time of use is theoretically about 0.41 mm. The minimum passage hole diameter measured according to the test method defined in “Union Voluntary Standard for Interdental Brush” (All Nippon Brush Industry Cooperative Association, 1999) is 0.60 mm.

  In this specification, “sample” refers to a property such as a sample collected for various tests, examinations, and analysis. Specifically, in the present embodiment, a plaque is assumed. .

  The diameter means a diameter unless otherwise noted, and when the edge is not circular, it means a portion where the distance between the edges is the smallest.

The fixed amount portion 4 has a through hole 9 formed at the tip thereof. The passage diameter (D ₄ ) of the through hole 9 is 0.55 mm, which is larger than the diameter (D ₁ ) of the shaft member 6 (0.3 mm), and the diameter (D ₂ ) of the sampling member 7 before bending. It is set smaller than 0 mm. Therefore, from the removed state shown in (1) of FIG. 2, a part of the sampling part 3 (the shaft member 6 and the sampling member 7) is attached by attaching the fixed quantity part 4 in the direction of the main body part 2 as shown by the arrow 11. It exposes from the through-hole 9, and transfers to the attachment state shown to (2) of FIG. At this time, the rear end portion 14 of the fixed amount portion 4 is loosely fitted to the main body portion 2.

  Moreover, as shown by the arrow 12, it is also possible to move and remove the fixed quantity portion 4 in the direction opposite to the attachment described above.

  In the present specification, the “passing diameter” means a diameter measured according to the measuring method of the diameter of the hole of the gauge defined in the above “union standard for interdental brushes”. When the diameter changes, it means the diameter of the narrowest part.

  Next, a quantitative process using the quantitative unit 4 of the sampling device 1 will be described.

  FIG. 3 is an enlarged view of the “A” portion shown in FIG. 2, wherein (1) is a state before the quantification process, (2) is an intermediate state of the quantification process, and (3) is after the quantification process. It is a state.

  First, before quantification, the shaft 6 or the bent sampling member 7 is brought into contact with a space between teeth (not shown) in the attached state shown in FIG. . As a result, as shown in (1) of the figure, the sample 5 collected in the collection unit 3 is held before the determination step.

Here, the through-hole 9 has a diameter D of the opening edge 20 on the upstream side (the side that comes into contact first when the quantification unit 4 moves in a certain direction, and in the case of the removal described in FIG. 5). _3, (a the side quantification unit 4 is in contact after when moving in a certain direction, in the case of removal to be described in FIG rear) downstream is set to be larger than the diameter D ₄ of the opening edge 21 of the . That is, the area of the surface formed by the upstream opening edge 20 is larger than the area of the surface formed by the downstream opening edge 21. In other words, the through hole 9 is formed so as to gradually narrow from the upstream side to the downstream side. Incidentally, passage diameter of the through-holes 9 at this time is 0.55mm point of _{D 4.}

  Next, as shown by the arrow 16, the quantification unit 4 is moved in the distal direction (upward in the figure) in a constant contact state with the sampling unit 3.

  With reference to (2) of the same figure which shows the middle state of a fixed_quantity | quantitative_assay process, the collection member 7 contacts the through-hole 9 from back, and passes the through-hole 9 gradually by bending. On the other hand, the sample (not shown) that is no longer held by the bent sampling member 7 is removed without passing through the through hole 9.

  At this time, as described above, the through hole 9 is formed so as to be gradually narrowed from the upstream side to the downstream side, so that when the sampling member 7 is passed through the through hole 9 from the upstream side, the bent sampling member 7 is Since it becomes easy to be drawn and the quantification proceeds gradually, the usability for quantification is improved.

  Then, by further moving the quantification unit 4 in the distal direction as indicated by the arrow 17, the quantification unit 4 is completely removed from the main body 2, and the state shown in (3) of FIG. The part of the sample 5 that has mainly passed through the through-hole 9 remains in the sampling part 3, is adjusted to a predetermined amount, and the quantitative process is completed.

  In addition, although the sample-collecting method which quantifies by removing the fixed_quantity | quantitative_assay part 4 from the main-body part 2 was demonstrated here, it can quantify similarly by attaching the fixed_quantity | quantitative_assay part 4 to the main-body part 2 conversely. At this time, if the through hole is tapered in the direction opposite to that of the present embodiment, it is preferable because the usability is improved.

  In this way, the collected sample 5 (plaque) is stably adjusted to a predetermined amount by the quantitative process. The sample 5 for which the quantification process has been completed is dispersed in a liquid such as water, and a test or the like for confirming the presence of periodontal disease bacteria using PCR or the like can be performed.

  As described above, according to the present invention, since the amount of the sample 5 remaining in the sampling unit 3 is stabilized, the accuracy of the inspection using the sample 5 is improved.

  Further, as described above, since the quantitative unit 4 is a cap body having the through-hole 9 at the tip thereof, the quantitative unit can be attached and detached without requiring a complicated operation, so that the simplicity of quantitative measurement is improved. .

  Further, as described above, the sampling portion 3 includes the shaft member 6 and the sampling member 7, and the through hole 9 has a predetermined dimensional relationship. Since the residual amount remaining can be designed, the certainty of the determination is improved.

  Furthermore, since the sampling member 7 includes the filament material as described above, sampling of the sample 5 and dispersion of the sample 5 after quantification are facilitated, so that sampling efficiency and usability of the sample 5 are improved. In the conventional sample collection device, the collected sample is often agglomerated, and it is difficult to disperse appropriately in liquids such as water, and the reaction related to the inspection may be hindered. is there.

  Next, FIG. 4 is a front view partly broken to show the central cross-sectional structure of the quantitative portion and the overcap in the sampling device according to the second embodiment of the present invention, and FIG. 5 is shown in FIG. FIG. 2 is a front view of the sample-collecting device, where (1) is in an attached state and (2) is in a removed state.

  Note that the basic configuration of the sampling device 31 according to the second embodiment is the same as that of the sampling device 1 according to the first embodiment described above, and therefore the following description will be focused on the differences.

  First, referring to FIG. 4, the sample collection device 31 further includes an overcap 38 that encloses the collection unit 33 and the determination unit 34. The overcap 38 is a cap body made of polypropylene, and a convex portion 41 projecting in a ring shape is formed on the entire rear side (in the direction from the overcap 38 to the fixed amount portion 34) at the rear end portion 40 thereof. In addition, a recessed portion 43 that is recessed inward is formed in the rear end portion 42 of the fixed amount portion 34. As a result, the overcap 38 is fitted to the outside of the fixed amount portion 34.

  Next, referring to (1) of FIG. 5, the collected sample 35 is held in the collection unit 33 as in the case of the first embodiment.

  When the overcap 38 is moved in the distal direction as shown by the arrow 45 while pressing the fitting portion with the fixed amount portion 34 (in the case of the present embodiment, the rear end portion 40 of the overcap 38), the overcap At the same time as 38, the quantification unit 34 can be removed, and the removal state shown in FIG.

  As a result, the quantification unit 34 has the same removal mode as in the first embodiment, so that the collected sample 35 is partially removed and adjusted to a predetermined amount.

  By providing the overcap 38 in this manner, the quantification unit 34 can be removed at the same time by removing the overcap 38 attached after the collection, so that the quantification unit 34 can be removed without directly touching, and the sample 35 The possibility of contamination is reduced and the certainty of the determination is improved. In addition, when the sample 35 is a substance whose contact is not recommended, such as plaque or blood, the safety is improved.

  In each embodiment of the present invention, each part of the sampling device is a specific material or a specific shape, but may be another material or another shape.

  In each embodiment of the present invention, each part of the sampling device has a specific dimensional relationship, but may have other dimensional relationships. In addition, when making a main-body part and an extraction part into an interdental brush-like, the diameter of the wire of the shaft member of an extraction member is 0.1 mm-1.0 mm from a viewpoint of making the extraction efficiency with respect to a tooth | gear and the fixed quantity efficiency of a plaque good. The diameter of the entire shaft material is preferably 0.2 mm to 3.0 mm, the diameter of the filament material is preferably 0.04 mm to 0.20 mm, and the diameter of the sampling member before bending is preferably Is preferably 1.0 mm to 15.0 mm, the minimum passage hole diameter of the sampling part is preferably 0.4 mm to 2.5 mm, and the passage diameter of the through hole of the quantitative part is 0.3 mm to 5. It is preferably 0 mm. Moreover, the passage diameter of the through hole is preferably 10% to 80%, more preferably 20% to 70%, relative to the diameter of the sampling member before bending. If comprised in this way, in a fixed_quantity | quantitative_assay process, a fixed_quantity | quantitative_assay part can be stably removed from a main-body part with a fixed contact state with respect to a collection | collection part, and quantitative efficiency becomes favorable.

  Furthermore, in each embodiment of the present invention, the main body part and the sampling part are interdental brush-like, but may be any of I-shaped, L-shaped, curved, etc. Depending on the case, it may be a brush-like object such as a swab, a cotton swab, or a sponge body. Further, although the sampling member includes the filament material, the sampling member may not include the filament material. Furthermore, the collection unit may not include a shaft member or a collection member. For example, even if the collection part is a cotton swab-like or dropper-like, the amount of the sample collected by passing through the through-hole can be adjusted, such a cotton swab-like or dropper-like collection part, Any shape that can be bent as long as it is deformed in the horizontal direction by pressure from the vertical or horizontal direction is included. Furthermore, the main body portion may be integrated with the sampling portion, and such a configuration is also included in one aspect of fixing the sampling portion to the main body portion.

  Furthermore, in each embodiment of the present invention, the quantification unit is moved in a certain direction with respect to the main body, but the position of the quantification unit may be fixed and the main body may be moved. It is included in the present invention as long as the quantitative unit moves relative to the main body.

  Furthermore, in each embodiment of the present invention, the through hole of the fixed amount portion is gradually narrowed from the upstream side to the downstream side, but other modes may be used. For example, one that gradually narrows from the downstream side to the upstream side can be mentioned.

  Furthermore, in each embodiment of the present invention, the fixed-quantity portion is a cap having a specific shape. However, it is sufficient that at least a part of the sampling portion is exposed from the through hole in the attached state.

  Furthermore, in each embodiment of the present invention, the sample collection method was implemented using a specific sample collection device, but other sample collection devices may be used, and the quantification unit is fixed to the collection unit. What is necessary is just to remove a part of sample collected by the collection part by attaching or removing in a contact state. In this way, since a desired amount of sample can be obtained regardless of the amount collected, the accuracy of the inspection using the sample is improved.

  Furthermore, in each embodiment of the present invention, the quantitative unit and the main body have a specific shape, but it is more preferable if a structure for guiding the quantitative unit to move in a certain direction is provided. For example, a shape in which the fixed amount portion follows the main body portion until the sampling portion completely passes through the through hole, or a guide groove is provided in the main body portion, and a protrusion that engages with this is provided in the fixed amount portion. .

  Hereinafter, the present invention will be described with reference to specific examples. In addition, this invention is not limited to the Example shown below.

<Test 1>
1. Preparation of equipment used The following equipment was prepared.
-PCR tube as a quantification part (manufactured by TreffLab, product name: PCR-SINGLE TUBE, PP, CLEAR, 0.2 mL)
-Interdental brush (Dental Pro (registered trademark) interdental brush I-shaped size 0 (4S) manufactured by Dental Pro Co., Ltd., size of the minimum passage hole diameter 0.6 mm, etc.) as the main body part and the sampling part The same as the sampling device of the first embodiment of
・ Precision pin vise (commercially available)
Paraffin (mixing ratio of solid paraffin and liquid paraffin: weight ratio 2 (solid): 3 (liquid), solid paraffin: manufactured by Kanto Chemical Co., Ltd., product name: Paraffin, content: 500 g, liquid paraffin: manufactured by Kanto Chemical Co., Ltd. , Product name: Liquid Paraffin, Content: 500 mL)
・ Precision electronic balance (commercially available)

2. Preparation of specimens Each specimen of Examples 1 to 3 was prepared by the following procedure.

  First, drills with diameters of 0.55 mm, 0.60 mm, and 1.10 mm were attached to the precision pin vise, and through holes with the diameters as the passage diameters were provided at the bottom of the PCR tube.

  Next, Example 1 (diameter 0.55 mm) and Example 2 (diameter 0.60 mm) were combined with an interdental brush and a PCR tube (diameter 0.55 mm and 0.60 mm) provided with through holes. ) Was prepared.

  Also, only the sampling part was removed from the interdental brush, and Example 3 (diameter 1.10 mm) was prepared in combination with a PCR tube having a diameter of 1.10 mm.

  Compared with Example 1 to Example 3, the PCR tube with a hole is such that the tip of the collection part of the interdental brush passes through the hole of the PCR tube from the inside, and the PCR tube becomes a cap-shaped quantitative part. Install in the test described below.

3. Test conditions Weight was measured using a precision electronic balance in a room at room temperature of 25 ° C. as follows.

First, the following procedure a to procedure d were performed before the quantitative process.
a. The weights of the interdental brushes of Example 1 and Example 2 and the sampling part of Example 3 were measured.
b. A PCR tube of each diameter was attached to Example 1 and Example 2, and the weight was measured.
c. Each sampling member of Examples 1 to 3 was passed through the paraffin and adhered to the whole, and 10 mg or more of the paraffin was adhered, and the paraffin was sampled and the weight was measured.
d. About Example 1 and Example 2, the weight measured by said procedure b was subtracted from the weight measured by said procedure c, and the weight of paraffin (collection amount) was calculated | required. Moreover, about Example 3, the weight measured by said procedure a was subtracted from the weight measured by said procedure c, and the weight of the paraffin (collected amount) was calculated | required.

  And as a fixed_quantity | quantitative_assay process, the PCR tube was removed from the interdental brush of Example 1 and Example 2, and a part of paraffin was removed. Moreover, the PCR tube was removed so as to pass in one direction with respect to the sampling part of Example 3, and a part of the paraffin was removed.

Next, the following procedure e to procedure h were performed after the quantification step.
e. The total weight of the interdental brush and paraffin (residual amount) of Example 1 and Example 2 was measured. Moreover, the total weight of the collection part of Example 3 and paraffin (residual amount) was measured.
f. The weight measured in the procedure a was subtracted from the weight measured in the procedure e to obtain the weight of paraffin (residual amount).

In addition, the above procedure a-procedure f was repeated 10 times in this order, and the arithmetic mean value was taken.
g. The standard deviation of 10 iterations was determined.
h. The coefficient of variation (%) for 10 iterations was determined.

4). Test results The test results are shown in Table 1 below.

以上の実施例１の結果から、定量工程により、定量部の貫通孔の通過径０．５５ｍｍとしたとき、約０．５ｍｇの試料を安定して採取できることを確認した。又、実施例２及び実施例３の結果からも同様に、貫通孔の通過径０．６０ｍｍ、１．１０ｍｍのとき、それぞれ約０．８ｍｇ、約４．５ｍｇの試料を安定して採取できることを確認した。

From the results of Example 1 above, it was confirmed that a sample of about 0.5 mg could be stably collected when the passage diameter of the through hole of the quantification part was 0.55 mm by the quantification process. Similarly, from the results of Example 2 and Example 3, when the through-hole passage diameters are 0.60 mm and 1.10 mm, it is possible to stably sample about 0.8 mg and about 4.5 mg, respectively. confirmed.

  Moreover, it was confirmed that the residual amount of the collected sample can be designed by appropriately setting the diameter of the passage diameter.

<Test 2>
1. Preparation of used instrument and test specimen As Example 4, the instrument basically similar to that of Example 1 of Test 1 described above was used except for the quantification unit.

  As the quantification unit, the quantification unit of the cap body described in the first embodiment was used.

  The passage diameter of the through-hole of the quantification part was 0.523 mm in actual measurement (10 arithmetic average values).

2. Test conditions A test basically similar to that of Example 1 of Test 1 described above was performed except for Procedure b and Procedure d.

  As a procedure b ′ instead of the procedure b, the weight of the fixed portion of the cap body was directly measured.

  Further, as the procedure d ′ instead of the procedure d, the weight measured in the procedure a and the procedure b ′ described above was subtracted from the weight measured in the procedure c to obtain the weight of paraffin (collected amount).

3. Test results The test results are shown in Table 2 below.

以上の実施例４の結果から、定量部の形状を変更しても、定量部に貫通孔があれば、定量工程において定量部を採取部に対し一定の接触状態で取り外すことができ、所定量の試料を安定して採取できることを確認した。

From the results of Example 4 above, even if the shape of the quantification part is changed, if the quantification part has a through hole, the quantification part can be removed from the sampling part in a constant contact state in the quantification step, and a predetermined amount It was confirmed that these samples could be collected stably.

DESCRIPTION OF SYMBOLS 1, 31 ... Sample collecting instrument 2 ... Main-body part 3, 33 ... Collecting part 4, 34 ... Fixed_quantity | quantitative_assay part 5, 35 ... Sample 6 ... Shaft material 7 ... Collecting member 9 ... Through-hole 20 ... Opening edge 21 ... Opening edge 38 ... Overcap In addition, the same code | symbol in each figure shows the same or equivalent part.

Claims (6)

The main body,
A collecting part fixed to the main body part and capable of collecting a sample;
A detachable attachment to the main body, and a fixed amount portion that exposes at least a part of the sampling part from the through-hole in the attached state;
The quantitative portion is provided with the through-hole at the tip thereof, and is a cap body that covers a part of the main body portion in an attached state,
The sampling unit includes a shaft extending in a first direction, and a bendable sampling member provided on at least a part of the shaft and collecting a sample,
The shaft member is configured in a rod shape as the entire shaft member by twisting a wire in a spiral shape,
The sampling member includes a filament material that is fixed in a spiral gap between the wires and protrudes in a direction different from the first direction from the shaft material,
A sample collection device in which the amount of the sample collected in the collection unit is adjusted when the collection member in the collection unit passes through the through hole by attaching or removing the quantitative unit from the main body. The passage diameter of the through hole is set to be larger than the diameter of the shaft member and smaller than the diameter of the sampling member before bending,
The sampling device according to claim 1, wherein a portion exceeding a predetermined amount is removed from the collected sample when the sampling member in the sampling portion passes through the through-hole by attaching or removing the quantitative portion. The quantitative unit moves from the upstream side in a certain direction to the downstream side in the attachment or removal,
The sampling tool according to claim 2, wherein the through hole has a surface area constituted by the upstream opening edge larger than an area area constituted by the downstream opening edge. The diameter of the sampling member before bending is 1.0 mm to 15.0 mm,
The minimum passage hole diameter of the sampling part is 0.4 mm to 2.5 mm,
The through-diameter of the through-hole of the quantitative portion is 0.3 mm to 5.0 mm,
The sampling diameter according to any one of claims 1 to 3, wherein the through-diameter of the through hole is 10% to 80% with respect to the diameter of the sampling member before bending.   The sample collection instrument according to any one of claims 1 to 4, further comprising an overcap that encloses the collection unit and the quantitative unit and fits outside the quantitative unit. A step of collecting a sample by a collecting portion fixed to the main body, and
A fixed amount part that is detachably attached to the main body part and exposes at least a part of the sampling part from the through hole in the attached state is attached to the main body part in a fixed contact state with respect to the sampling part or from the main body part A step of removing a part of the sample collected in the collection part by removing,
The quantitative portion is provided with the through-hole at the tip thereof, and is a cap body that covers a part of the main body portion in an attached state,
The sampling unit includes a shaft extending in a first direction, and a bendable sampling member provided on at least a part of the shaft and collecting a sample,
The shaft member is configured in a rod shape as the entire shaft member by twisting a wire in a spiral shape,
The sample collection method, wherein the collection member includes a filament material fixed in a spiral gap between the wires and protruding from the shaft member in a direction different from the first direction.

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