JP6351187B2 - Sample collection tool, sample collection method, sample inspection method - Google Patents

Description

  The present invention relates to a sampling tool used for sampling such as stool collection, a sampling method using the sampling tool, and a sample inspection method.

As a sample collection tool used for sample collection such as stool collection, for example, one disclosed in Patent Document 1 is known.
The sample collection tool (sample collection device) described in Patent Document 1 has a tubular body having one end closed and an opening at the other end, a sealing plug capable of sealing the opening of the tubular body, and a size that can be accommodated in the tubular body. It is the structure which has the collar part for sampling of the length, and the handle part which connects a collar part and a sealing stopper.
In this sampling tool, after a sample such as feces is collected on the heel of the handle tip (on the side opposite to the sealing plug), the heel and handle on which the sample is placed are inserted into the tubular body through the opening. The collected sample can be stored in the tubular body by sealing the opening of the tubular body with a sealing plug.

FIG. 2 of Patent Document 1 discloses a sampling tool having a configuration in which an angle θ1 formed by the collar portion and the handle portion is 0 to 20 degrees. Further, FIG. 6 of Patent Document 1 discloses a sampling tool having a configuration in which an angle θ2 formed by the collar portion and the handle portion is about 40 degrees.
The handle portion of the sample collection tool shown in FIGS. 2 and 6 of Patent Document 1 is arranged at the center in the cross-sectional direction perpendicular to the axial direction of the tubular body when the opening of the tubular body is sealed with a sealing plug. Moreover, the extension dimension from the handle part tip of the collar part of this sampling tool is much smaller than the extension dimension from the sealing plug of the handle part.

JP-A-6-201684

  The sample collection tool disclosed in FIGS. 2 and 6 of Patent Document 1 has a small sample collection amount because the size of the buttock is small. For this reason, for example, there arises a disadvantage that it cannot be applied to collection of a sufficient amount of sample (such as stool) for various tests.

  The problem to be solved by the present invention is that a sample collection tool that can easily realize an increase in the amount of sample collection and is advantageous for preventing the sample from adhering to the stopper during sample collection work, a sample collection method using the sample collection tool, It is to provide a sample inspection method.

In order to solve the above problems, the present invention provides the following aspects.
In the first aspect, a container in which an opening is formed on the other end side opposite to the bottom wall on the one end side, and a sampling protrusion for collecting a sample from a plug body that is desorbed on the other end side of the container are provided. A plug with a plastic sampling piece that extends, the plug being capable of sealing the opening of the container and being inserted into the opening when the opening of the container is sealed. A cylindrical side wall that protrudes from the inner peripheral portion of the annular top plate portion that is in contact with the peripheral edge of the opening of the container in the stopper to one side in the axial direction thereof. And a plug bottom wall portion that closes the protruding end side of the cylindrical side wall portion of the inner space of the cylindrical side wall portion and forms the bottom surface of the plug body, and the sampling protrusion is a bottom of the plug body A sampling piece portion extending from the outer periphery of the surface or the tip of the support piece projecting from the outer periphery of the bottom surface, wherein the sampling piece is the plug Opposite the central axis of the plug body through the central axis of the plug body, which coincides with the central axis of the opening of the container when the opening of the container is sealed from the bottom or the tip of the support piece The sampling projection piece is entirely the sampling piece portion, or the extension length from the support piece portion and the support piece portion is that of the plug body. The sampling tool having the sampling piece portion larger than the projecting dimension of the support piece portion from the bottom surface .
A second aspect is the sample collecting tool according to the first aspect, wherein a tip of the collection piece portion is located on an extension of a central portion of the bottom surface in a central axis direction of the plug body.
A third aspect is characterized in that a base end portion on the plug body side of the sampling piece portion is formed in an arc plate shape extending in a curved cross-sectional shape along the outer peripheral portion of the bottom surface of the plug body. It is a sampling tool of the 1st or 2nd aspect .
A fourth aspect is the sampling device according to any one of the first to third aspects, wherein a concave portion into which a finger can be inserted is formed in the plug body from the side opposite to the bottom surface. is there.
A fifth aspect is a sampling method, wherein a sample is collected using the sampling tool of any one of the first to fourth aspects.
A sixth aspect is a sample inspection method characterized by collecting a sample by the sample collection method of the fifth aspect and subjecting the collected sample to inspection.

According to the sample collecting tool according to some embodiments of the present invention, it is possible to easily increase the size of the collection piece portion of the collection protrusion and increase the sample collection amount due to the configuration.
The sampling piece of the sampling tool is extended from the outer periphery of the bottom surface of the plug. When the stopper with a sampling piece is oriented so that the sampling protrusion is located below the center of the bottom surface of the stopper, the portion of the stopper located below the sampling protrusion is located above the sampling protrusion. The size is small compared to the part located at. For this reason, the sample collection tool is used to carry out operations such as scooping up the sample on the collection protrusion whose tip is slid on the mounting surface on which the sample is placed. By performing in the direction positioned at the position, it is easy to secure the distance from the sample located below the plug body, which is advantageous for preventing the sample from adhering to the plug body.

1 is an exploded front sectional view showing a sampling tool of one embodiment according to the present invention. It is a front sectional view which shows the state which sealed the container opening part with the stopper of the stopper with a collection piece of the sample collection tool of FIG. It is a perspective view which shows the structure which looked at the stopper with a collection piece of the sample collection tool of FIG. 1 from the collection protrusion piece side. It is a bottom view which shows the structure which looked at the stopper with a collection piece of the sample collection tool of FIG. 1 from the protrusion piece for collection. It is a side view of the stopper with a collection piece of the sample collection tool of FIG. 1, and shows the structure which looked at the stopper with a collection piece from the curved inner peripheral side (concave surface side) of the arcuate plate-like collection piece. It is a figure explaining the raising operation of the stopper with a collection piece in the sample collection method using the sample collection tool of FIG.

Several preferred embodiments of the present invention will be described below with reference to the drawings.
1 and 2 show a sampling tool 10 according to a preferred embodiment of the present invention.
The sample collection tool 10 illustrated in FIGS. 1 and 2 includes a container 20 and a plug 30 with a collection piece configured so that a collection protrusion 32 extends from a plug 31 that can seal the opening 21 of the container 20. Have.

  The sample collection tool 10 collects samples such as food, human feces, human secretions, organic wastes such as soil, mud, and garbage by using the collection protrusion 32 of the plug 30 with the collection piece. The container 20 can be stored and stored. That is, it can be used for collecting various samples regardless of whether they are hygienic samples or non-hygienic samples.

  The container 20 is formed of a material that can ensure water shielding, such as plastic, glass, metal, and the like. Further, the stopper 30 with the collection piece is a material that can ensure the strength to stably perform the piercing operation of the collection protrusion 32 to the stool or the like without causing large deformation, destruction, damage or the like to the stopper 30 with the collection piece. Formed by. Examples of the material for forming the stopper 30 with the collection piece include plastic and metal.

  It is preferable that the container 20 of the sample collection tool 10 and the stopper 30 with the collection piece are each formed of a material that can be sterilized by γ-ray irradiation. Moreover, the material for forming the container 20 of the sampling tool 10 and the stopper 30 with the sampling piece is preferably plastic in terms of cost reduction.

  The container 20 and the stopper 30 with the collection piece of the sample collection tool 10 illustrated in FIGS. 1 and 2 are plastic members that can be sterilized by γ-ray irradiation. Examples of the plastic forming the container 20 and the stopper 30 with the collection piece include general-purpose resins such as polycarbonate, polyethylene terephthalate, acrylic, polyvinyl alcohol, polyamide, polysulfone, ABS resin, and polystyrene.

The container 20 of the sampling tool 10 has a cylindrical wall portion 22 and a bottom wall 23 that closes one axial end thereof, and the other end opposite to the bottom wall 23 in the axial direction of the cylindrical wall portion 22 is open. It is formed in the bottomed cylindrical shape (one side bottomed cylindrical shape). An opening 21 of the container 20 indicates an opening on the other end side of the cylindrical wall portion 22.
The cylindrical wall portion 22 (hereinafter also referred to as a container cylindrical wall portion) of the container 20 of the sample collection tool 10 illustrated in FIGS. 1 and 2 is specifically cylindrical. The container 20 is formed in a cylindrical shape with one bottom. The container 20 is an integrally molded product made of plastic.

As shown in FIGS. 1 to 3, the stopper 30 with the sampling piece of the sampling tool 10 includes a stopper 31 and a sampling protrusion 32 extending from the stopper 31.
The stopper 31 of the stopper 30 with the collection piece illustrated in FIGS. 1 to 3 is an annular shape that can abut on the entire periphery of the mouth portion around the opening 21 of the container 20 (hereinafter also referred to as the container mouth portion). The top wall 33 includes an insertion protrusion 34 that can be inserted into and removed from the opening 21 of the container 20 (hereinafter also referred to as a container opening), and a screwing wall 35.

The container mouth edge of the container 20 of the sample collection tool 10 illustrated in FIGS. 1 to 3 is specifically the end of the cylindrical wall 22 around the container opening 21 (hereinafter referred to as the open end of the cylindrical wall 22). Point).
On the one side in the axial direction of the annular top wall 33, an abutting surface 33a that abuts on the container mouth edge is formed. The contact surface 33 a of the annular top wall 33 can contact the entire circumference of the end surface of the cylindrical wall 22 around the container opening 21.

The contact surface 33 a of the plug 31 illustrated in FIGS. 1 to 3 is formed in an annular shape along a circumference coaxial with the inner circumference of the annular ceiling wall portion 33. The contact surface 33a of the plug 31 illustrated in FIGS. 1 to 3 is formed as a flat surface perpendicular to the central axis of the inner periphery of the annular top wall 33.
In the present specification, the plug body 31 passes through the center of curvature of the annular contact surface 33a and is perpendicular to an imaginary plane including the circumference centered on the contact surface 33a (hereinafter referred to as the contact surface 33a). Are also treated as the central axis 31L of the plug 31.
In the plug body 31 illustrated in FIGS. 1 to 3, the central axis of the inner periphery of the annular ceiling wall 33 coincides with the plug body central axis 31 </ b> L.

  The annular top wall 33 of the plug 31 illustrated in FIGS. 1 to 3 is specifically formed in a ring plate shape. The inner periphery and the outer periphery of the annular ceiling wall 33 are concentrically formed coaxially with the plug central axis 31L.

  As shown in FIG. 2, the inner diameter of the annular top wall 33 is smaller than the inner diameter of the container opening 21. The outer diameter of the annular ceiling wall portion 33 of the ring plate-shaped plug body 31 illustrated in FIGS. 1 to 3 matches the outer diameter of the container mouth edge or is slightly larger than the outer diameter of the container mouth edge.

As shown in FIGS. 1 to 3, the insertion protrusion 34 of the plug 31 includes a cylindrical side wall 34 a that protrudes from the inner periphery of the annular top wall 33 to one side in the axial direction, and an inner side of the cylindrical side wall 34 a. It has a plug body bottom wall part 34b that closes the protruding side of the cylindrical side wall part 34a of the space.
The cylindrical side wall portion 34 a is formed in a cylindrical shape extending coaxially with the inner peripheral portion of the annular top wall portion 33.

In the plug body 31 shown in FIGS. 1 and 2, the surface opposite to the contact surface 33a of the annular top wall 33 is hereinafter also referred to as a plug body top surface 33b.
As shown in FIGS. 1 and 2, the side opposite to the plug bottom wall 34 b of the inner space 34 c of the insertion protrusion 34 is opened. A region on the inner side from the inner periphery of the annular ceiling wall 33 (the radially inner side of the inner periphery of the annular ceiling wall 33) is an opening of the inner space 34 c of the insertion protrusion 34. The inner space 34c of the insertion protrusion 34 is a recess that is recessed from the inner periphery of the plug top surface 33b. Hereinafter, the inner space 34c of the insertion protrusion 34 is also referred to as a stopper recess.
Further, the plug recess 34c of the plug 30 with the collection piece shown in FIGS. 1 and 2 is inserted into the user's finger of the plug 30 with the collection piece from the opening side (the side opposite to the plug bottom wall 34b). It is formed in a possible size.

  As shown in FIGS. 1 to 4, the screwing wall portion 35 protrudes from the entire outer periphery of the annular top wall portion 33 to the same side as the cylindrical side wall portion 34 a in the axial direction (center axis direction) of the plug body 31. It is formed in a cylindrical shape. The annular top wall 33 is located inside the end of one side in the axial direction of the cylindrical threaded wall 35. The screwing wall portion 35 of the plug body 31 shown in FIGS. 1 to 4 is specifically formed in a cylindrical shape centered on the plug body central axis 31L.

  As shown in FIGS. 1 to 4, an inner screw portion 35 a is formed on the inner peripheral side of the screwing wall portion 35 of the plug body 31. The inner screw portion 35a is a female screw portion centered on the plug body central axis 31L. On the outer peripheral side of the opening end of the cylindrical wall portion 22, an external screw portion 22 a that can be screwed with the internal screw portion 35 a of the screwing wall portion 35 of the plug 31 is formed.

The plug body 31 can be screwed onto the cylindrical wall portion 22 of the container 20 by screwing the inner screw portion 35a of the screwing wall portion 35 with the outer screw portion 22a on the outer periphery of the opening end portion of the cylindrical wall portion 22 of the container 20.
The plug body 31 is screwed to the container cylinder wall portion 22 with its center axis 31L aligned with the center axis of the opening end of the container cylinder wall portion 22.

As shown in FIGS. 1 to 4, the sampling protrusion 32 of the plug 31 has a surface 34 d (on the opposite side of the annular top wall 33 of the plug bottom wall 34 b of the insertion protrusion 34 of the plug 31 ( A bottom surface (hereinafter also referred to as a bottom surface of the plug body) extends from the outer peripheral portion to the side opposite to the annular ceiling wall portion 33.
As shown in FIGS. 1 to 3, the sampling protrusion 32 extends from the support piece portion 32 a on the plug body 31 side and the tip of the support piece portion 32 a (the end opposite to the plug body 31). And a collection piece 32b.

The support piece 32a protrudes (extends) from a part of the outer periphery of the plug bottom surface 34d (specifically, the plug bottom wall 34b) to the side opposite to the plug top surface 33b.
The support piece 32a of the sampling protrusion 32 of the plug 30 with the sampling piece shown in FIGS. 1 to 4 protrudes along the axial direction of the plug 31 from the plug bottom 34d in an arcuate section along the outer periphery of the plug bottom 34d. It is formed in a circular arc plate shape. 1 to 4 protrudes with a constant cross-sectional dimension over the entire length in the protruding direction from the plug bottom surface 34d. The cross-sectional dimension of the support piece portion 32a in the outer peripheral direction of the plug bottom surface 34d is preferably 1/8 or more and half or less of the outer periphery of the plug bottom surface 34d, and is 1/6 or more and half of the outer periphery of the plug bottom surface 34d. The following is more preferable.

As shown in FIGS. 3 and 4, the collection piece 32b of the collection protrusion 32 of the stopper 30 with the collection piece is formed in an arc plate shape extending in a curved cross-sectional shape from the tip of the support piece 32a. Portions other than the tip of the collection piece portion 32b extend with the same cross-sectional dimensions as the support piece portion 32a. The arcuate plate-shaped sampling piece portion 32b has a concave curved surface on the curved inner peripheral side in contact with a curved curved surface on the curved inner peripheral side of the support piece portion 32a, and a convex curved surface on the curved outer peripheral side on the curved outer peripheral side of the support piece portion 32a. It is formed in a cross-sectional curved shape in a direction in contact with the convex curved surface.
A cross section perpendicular to the extending direction of the collection piece portion 32b of the base end portion (end portion on the plug body 31 side) of the collection piece portion 32b extends in a circular arc shape along the outer peripheral portion of the bottom surface of the plug body 31.

As shown in FIGS. 1 and 2, the collection piece portion 32b is formed from the distal end (protrusion end) of the support piece portion 32a protruding from the plug bottom surface 34d toward the opposite side of the plug body 31 to the plug body central axis 31L. In contrast to the plug body central axis 31L, it extends to the opposite side (side opposite to the tip of the support piece 32a).
The collection piece portion 32b is supported with respect to the plug body 31 via the support piece portion 32a.

  The support piece 32a of the plug 30 with the collection piece shown in FIGS. 1 to 4 protrudes in the axial direction of the plug 31 from the plug bottom surface 34d. For this reason, the collection piece part 32b is inclined and extended not only with respect to the plug body central axis 31L but also with respect to the protruding direction from the plug bottom face 34d of the support piece part 32a.

The protruding dimension of the support piece part 32a of the stopper 30 with the collection piece 30a of the embodiment illustrated in FIGS. 1 to 4 from the bottom face 34d is the extension dimension of the collection piece part 32b (extension length from the support piece part 32a). Is smaller than The projecting dimension of the support piece portion 32a from the bottom surface 34d of the stopper is ensured to be, for example, 15 to 35% of the extending dimension of the collection piece portion 32b.
The inclination angle θ (see FIG. 1) of the collection piece portion 32b of the collection piece-attached plug body 30 with respect to the protruding direction from the bottom surface 34d of the support piece portion 32a is preferably 10 to 70 degrees, and 30 to 60 degrees. It is more preferable that

As shown in FIGS. 4 and 5, the distal end portion of the collection piece portion 32b is reduced as the extension direction dimension of the curved cross section perpendicular to the extension direction of the collection piece portion 32b goes to the distal end side of the collection piece portion 32b. It is formed in a tapered shape.
The distal end of the tapered collection piece 32b has an advantage of facilitating the puncture of the collection piece 32b into solid feces or the like.

The fact that the cross-sectional dimension in the outer peripheral direction of the plug bottom surface 34d of the arcuate plate-like support piece 32a is less than half and the cross-sectional dimension of the collection piece 32b is the same as that of the support piece 32a is that This is advantageous in terms of easy molding and cost reduction.
In addition, the cross-sectional dimension of the collection piece part 32b does not necessarily need to be the same as that of the support piece part 32a, and may differ from the cross-sectional dimension of the support piece part 32a. The sampling protrusion 32 has, for example, an extending direction dimension of a curved cross section perpendicular to the extending direction of a portion other than the tip end portion of the arc plate-shaped sampling piece portion 32b, and that of the arc plate-shaped support piece portion 32a. A configuration larger than the dimension of the curved cross section in the extending direction can also be adopted.

The stopper 30 with the sampling piece removed from the container 20 inserts the sampling protrusion 32 and the insertion protrusion 34 of the stopper 31 into the container opening 21, and the stopper 31 is opened at the opening of the container cylinder wall 22. And the inner threaded portion 35a of the threaded wall portion 35 is screwed into the outer threaded portion 22a of the opening end portion of the container tube wall portion 22 so that the plug 31 is opened at the open end of the container tube wall portion 22. Can be screwed to the part. Then, the stopper 30 with the collection piece is screwed into the opening end of the container cylinder wall 22 of the stopper 31 screwed to the opening end of the container cylinder wall 22 so that the annular top wall 33 is pressed against the container mouth. The container opening 21 can be sealed by tightening the stopper 31 to the container 20 with the contact surface 33a in contact. Further, it is possible to enhance the sealing performance by sandwiching packing 33 (a sealing member, a sealing member, a sealing member, etc.) made of a material such as silicon or rubber in 33a.
The stopper 31 screwed into the opening end of the container tube wall 22 can be detached from the container 20 by rotating the container 31 against the opening of the container tube wall 22 in the direction opposite to that during screwing. .

When the container opening 21 is hermetically sealed by screwing and tightening the stopper 31 of the stopper 30 with the collection piece into the opening end of the container cylinder wall 22, The tip is formed with an extension dimension that reaches the vicinity of the bottom wall 23 of the container 20.
When the container opening 21 is sealed by the stopper 31 of the stopper 30 with the sampling piece, the tip of the sampling protrusion 32 of the stopper 30 with the sampling piece is near the center axis of the container cylinder wall 22 (the container cylinder wall 22 at or near the center axis).

The stopper 31 of the stopper 30 with a collection piece illustrated in FIGS. 1 and 2 has a sealing auxiliary protruding wall 36 that protrudes from the annular top wall 33 to the side opposite to the stopper top surface 33b. The sealing auxiliary protruding wall 36 is formed in a ring shape centered on the plug body central axis 31L. However, the sealing auxiliary protruding wall 36 is smaller in diameter than the screwing wall part 35 and is formed to be separated from the screwing wall part 35 toward the plug body central axis 31L. The contact surface 33 a of the annular ceiling wall 33 is located between the auxiliary sealing projection wall 36 and the screwing wall 35 on the opposite side of the annular ceiling wall 33 from the plug top surface 33 b.
Between the sealing auxiliary projecting wall 36 and the screwing wall portion 35, the container cylindrical wall portion 22 opening end is inserted when the container opening portion 21 is sealed by the stopper 31. The sealing auxiliary protruding wall 36 abuts on the inner peripheral surface of the opening end of the container cylinder wall portion 22 inserted between the sealing auxiliary protruding wall 36 and the screwing wall portion 35, so that the sample 42 in the container 20 is in the container lip. In order to prevent leakage from between the opening portion and the plug body 31 (specifically, the annular ceiling wall section 33), it plays a role of improving the sealing performance of the container opening 21 by the plug body 31.
In addition, the structure which does not have the sealing auxiliary | assistant protrusion wall 36 is also employable for the stopper with a collection piece.

The stopper 30 with the collection piece of the sample collection tool 10 is used for collecting a sample such as stool in a state of being removed from the container 20 (a state of being detached). Sample collection by the plug 30 with the state collection piece removed from the container 20 is performed by, for example, operating the user with a finger holding the plug 31 and collecting a sample such as feces in the collection piece 32b.
The sample collection tool 10 includes a plug 31 that is inserted into the container opening 21 together with the collected sample, and the plug 31 that is screwed into the opening end of the container tube wall 22. By tightening the container 20 and sealing the container opening 21, the collected sample can be stored and stored in the container 20.

A sample of solid stool or the like collected in the collection piece portion 32b of the stopper 30 with the collection piece is mainly between the collection piece portion 32b of the collection projection piece 32 inserted into the container cylinder wall portion 22 and the container 20. The sample is collected and stored in the sample collection tool 10.
The sample collection tool is a sample collection part 32b that is obtained when only the collection piece 32b of the stopper 30 with the collection piece is changed from the sample collection tool 10 shown in FIGS. 1 and 2 and the container opening 21 is sealed by the stopper 31. A configuration in which the base end side portion of the tip of the tube intersects the central axis of the container tube wall portion 22 and the tip of the collection piece portion 32b is disposed close to the inner peripheral surface of the container tube wall portion 22 (hereinafter referred to as the proximity of the tip of the collection portion) (Also known as a shape collection tool). However, in the case of the sample collection tool 10 shown in FIGS. 1 and 2, the space between the collection piece portion 32 b and the inner peripheral surface of the container cylindrical wall portion 22 when the container opening 21 is sealed with the stopper 31 is provided. It is possible to secure a larger size than that of the sampling part tip proximity type sampling tool. For this reason, when the container opening 21 is sealed by the stopper 31, the sample collection tool 10 having a configuration in which the tip of the collection protrusion 32 is arranged near the central axis of the container cylinder wall portion 22 is provided with the collection portion tip proximity collection. It is advantageous in increasing the sample capacity compared to the tool.

The protruding dimension from the bottom surface 34d of the support piece 32a of the sampling part tip proximity type collecting tool is ensured to be, for example, 5 to 35% of the extension dimension of the collecting piece part 32b.
The inclination angle θ (see FIG. 1) of the collection piece portion 32b of the collection portion tip proximity collection tool with respect to the protruding direction of the support piece portion 32a from the plug bottom surface 34d is preferably 10 to 70 degrees, and preferably 30 to 60 degrees. More preferably.

  When the sample is stool, the sample collection by the stopper 30 with the collection piece removed from the container 20 is taken out to the collection piece portion 32b stabbed into the solid or clay-like stool and placed on which the stool is placed. It can be realized by scooping up the collection piece 32b moved along the surface (see FIG. 6) or the like.

FIG. 6 is a diagram for explaining the sample collecting method of the present invention, and in particular, a scooping operation for picking up and collecting the sample 42 on the mounting surface 41 extending in the horizontal direction onto the collection piece 32b. FIG. As shown in FIG. 6, the scooping operation is performed by placing the stopper 30 with the sampling piece removed from the container 20 so that the stopper central axis 31L is closer to the stopper 31 than the sampling piece 32b. The tip of the collection piece 32b is brought into contact with the mounting surface 41 in a direction inclined with respect to the mounting surface 41 so that the separation distance from the surface 41 is increased. Moreover, the stopper 30 with the collection piece is used in such a direction that the portion of the stopper bottom wall portion 34b that is continuous with the base end portion of the collection protrusion 32 is located at the lowermost portion of the stopper bottom wall portion 34b.
The scooping operation is performed by moving the stopper 30 with the sampling piece along the mounting surface 41 while keeping the tip of the sampling piece portion 32b in contact with the mounting surface 41 in the above-described direction. A sample 42 is picked up from 41 and collected. The sample 42 is collected on the concave curved surface side of the collection piece 32b.

In the scooping operation of the sample 42 in FIG. 6, the stopper 31 (specifically, the screwing wall portion 35) of the stopper 30 with the collection piece and the finger of the user holding the stopper 31 are placed on the mounting surface 41. In order to prevent the sample from adhering to the upper sample 42, the plug 31 is separated from the mounting surface 41 and supported. Note that the sample may be dispersed over a wide range of the mounting surface 41. Supporting the plug body 31 by separating it upward from the placement surface 41 means that the finger of the user who holds the plug body 31 (specifically, the screwing wall portion 35) or the plug body 31 is placed on the placement surface 41. The sample is prevented from coming into contact with a sample that is dispersed in a wide range.
On the other hand, in the scooping operation, there is a case where the sample is desired to be scooped up by bringing the collection piece 32b close to the placement surface 41 in order to collect the sample on the collection piece 32b.

The sampling protrusion 32 of the stopper 30 with the sampling piece extends from the outer periphery of the bottom wall 34b of the stopper.
Here, the plug 30 with the collection piece is modified so that the collection protrusion 32 extends from the central portion of the plug bottom wall portion 34b (the plug bottom surface 34d) (hereinafter, the collection portion central extension type plug). (Also called body).
The stopper 30 with the sampling piece is used for a scooping operation in a direction in which the base end portion of the sampling bottom 32 of the stopper bottom wall 34b is located at the lowermost part of the stopper bottom wall 34b, The distance (height) from the mounting surface 41 of the plug body 31 can be ensured to be large (high) as compared with the collection part central extension type plug body. That is, when the stopper 30 with the sampling piece is used for a scooping operation in a direction in which the base end of the sampling protrusion 32 of the stopper bottom wall 34b is located at the lowermost part of the stopper bottom wall 34b. The distance (height) from the mounting surface 41 of the plug body 31 is ensured to be larger (higher) than that of the sampling section central extension type plug body having the same inclination angle with respect to the mounting surface 41 of the sampling piece portion 32b. it can.

  For this reason, the stopper 30 with the sampling piece has the stopper 31 and the user's finger holding the stopper 31 on the mounting surface 41 during the scooping operation compared to the central extension extending stopper of the sampling part. This is advantageous in that it prevents the sample from adhering to the sample 42. The stopper 30 with the collection piece is more advantageous for improving the workability of collecting the sample by the scooping operation than the central extension plug body of the collection part.

The handle portion of the sample collection tool (sample collection device) described in Patent Document 1 is tubular when the opening of the tubular body in the sealing plug is sealed, as can be seen with reference to FIGS. 2 and 6 of Patent Document 1. It extends from the center of the surface facing the body space.
Compared with the sample collection tool described in Patent Document 1, the stopper 30 with the collection piece is advantageous in improving workability of sample collection by the scooping operation.

  The extension dimension from the handle | tip part of the handle part of the collar part of the sampling tool of patent document 1 is remarkably small compared with the extension dimension from the sealing stopper of a handle part. On the other hand, the stopper 30 with the sampling piece whose protrusion dimension from the stopper bottom surface 34d of the support piece 32a is smaller than the extension dimension of the sampling piece part 32b is increased in size and sampling of the sampling piece part 32b. It is advantageous to increase the amount. In addition, the stopper 30 with the sampling piece has the workability of sampling by the scooping operation even if the protruding dimension of the support piece 32a from the bottom surface 34d of the supporting piece 32a is 15 to 35% of the extension dimension of the sampling piece 32b. Can be secured satisfactorily.

  In addition, the sample collection tool described in Patent Document 1 has a horizontal direction when the extension dimension from the front end of the handle part of the collar part is larger than the extension dimension from the sealing plug of the handle part. The distance (height) of the sealing plug from the mounting surface 41 is reduced during the scooping operation for scooping up the sample on the mounting surface 41 extending to the surface. As a result, the sample collection tool described in Patent Document 1 has poor sample collection workability due to the scooping operation.

  The operation of the stopper 30 with the collection piece is performed, for example, by the user holding only the outer peripheral portion (specifically, the screwing wall portion 35) of the stopper 31 with a plurality of fingers, but is not limited thereto. The operation of the stopper 30 with the sampling piece is performed by using, for example, fingers inserted into the stopper recess 34c of the stopper 31 and by using a plurality of fingers, the outer surface of the screwing wall 35 of the stopper 31 and the stopper recess 34c. You may hold | grip the part located between.

  For example, when the plug 30 with the collection piece is inserted into a narrow work space and collected by a scooping operation, it may be difficult to wrap the fingers under the plug 31 or both left and right sides. However, the stopper 30 with the sampling piece is used for the operation of scooping up the outer surface of the screwing wall 35 of the stopper 31 and the stopper recess 34c using the fingers inserted into the stopper recess 34c of the stopper 31. It is also possible to efficiently perform a scooping operation in a narrow work space by gripping a portion located between the fingers.

Next, the invention of the sample inspection method using the sampling tool 10 of the present invention will be described. In the sample inspection method of the present invention, first, the sample collection method shown in FIG. 6 is executed. Specifically, for example, in FIG. 6, if the sample 42 is the stool of the subject, the stopper with a collection piece 30 is used according to the embodiment described in FIG. 6, and the sample 42 (feces) is scooped up by the collection piece portion 32 b. To collect.
In that state, the stopper 30 with the sampling piece is inserted into the container opening 21 with the sampling protrusion 32 and the insertion protrusion 34 of the stopper 31, and the stopper 31 is relative to the opening end of the container cylinder wall 22. By rotating, the inner threaded portion 35a of the threaded wall portion 35 is screwed into the outer threaded portion 22a at the opening end portion of the container tube wall portion 22, and the stopper 31 is screwed into the opening end portion of the container tube wall portion 22. Thereby, the sampling tool 10 is in the state shown in FIG. However, although not shown in FIG. 2, the sample 42 (feces) collected in the collection piece 32 b exists in the internal space of the container 20.
In this state, the collected sample 42 (feces) can be subjected to an auxiliary test for cedar pollinosis described in, for example, Japanese Patent No. 5264239. In this case, the number of bacteria of one or more bacterial species selected from the group consisting of Bacteroides Uniformis and Bacteroides kaykae in the collected sample 42 (stool) is less than 1 million per gram of sample 42 (stool). In this case, it is determined that there is a high possibility that the subject is suffering from cedar pollinosis.

  As described above, the sample collection method of the present invention can be used in various inspection methods. For example, if the sample 42 is human stool, the water content in stool, amino acids, fatty acids, etc. Ingredient composition of intestinal metabolites (including intestinal secretion), bacteriological methods (including culture methods) or PCR methods, bacterial flora by next-generation sequencers, etc. Can do. Moreover, if the sample 42 is a food, it can be used for tests such as hygiene tests such as nutrient composition, bacteria count, and environmental hormone measurement. Furthermore, if the sample 42 is soil, mud, etc., it can be used for inspections such as environmental tests.

Although the present invention has been described based on the best mode, the present invention is not limited to the above-described best mode, and various modifications can be made without departing from the gist of the present invention.
The collection protrusion of the stopper with the collection piece is not limited to a configuration in which the projecting dimension of the support piece portion 32a from the bottom surface 34d of the support piece 32a is smaller than the extension dimension of the collection piece portion 32b. A configuration in which the projecting dimension from the bottom surface 34d is equal to or greater than the extending dimension of the collection piece 32b can also be employed.
The collection piece of the stopper with the collection piece is not limited to the configuration having the support piece portion 32a, and a configuration in which the support piece portion 32a does not exist and the collection piece portion extends directly from the bottom surface of the plug body can also be adopted. .

The insertion protrusion 34 constituted by the cylindrical side wall 34a and the plug bottom wall 34b of the plug 31 of the plug 30 with the sampling piece illustrated in FIG. 1, FIG. 2, etc., and the annular top wall 33 are: A plug body top wall portion that closes one axial end of the threaded wall portion 35 of the plug body 31 is configured.
However, it is possible to adopt a configuration in which the plug body concave portion 34c does not exist in the plug body of the plug body with the collection piece.
The stopper of the stopper with the sampling piece has a structure in which the stopper recess 34c is not formed and has a solid insertion protrusion, or an insertion protrusion does not exist and closes one end in the axial direction of the screwing wall 35. A configuration having a disk-shaped plug top wall portion can also be adopted. The plug body that is not formed with the plug body recess 34c and has a solid insertion protrusion includes a disk-shaped plug body top wall portion that closes one end portion in the axial direction of the screwing wall portion 35, and the plug body ceiling. It is the structure which has the solid insertion protrusion which protrudes from the surface direction center part of a wall part to the side from which the screwing wall part 35 protrudes.

In the plug body top wall portion of the plug body 30 with the collection piece illustrated in FIGS. 1 and 2, only the surface opposite to the contact surface 33 a of the annular top wall portion 33 is the plug body top surface 33 b. The plug body top surface of the plug body having a disc-shaped plug body top wall portion that closes one end portion in the axial direction of the screw wall portion 35 is the side from which the screw wall portion 35 of the plug body top wall projects. The entire surface on the opposite side.
The outer periphery of the surface of the disk-shaped plug top wall opposite to the top surface of the plug body that closes one end in the axial direction of the screwing wall 35 contacts the container mouth when the container opening is sealed. A contact surface to be contacted is secured.
In addition, the sampling projection of the stopper with a sampling piece having a disk-like stopper top wall that closes one end in the axial direction of the screwing wall 35 faces the inner space of the container when the container opening of the stopper is sealed. It extends from the outer periphery of the bottom surface of the plug body to the side opposite to the top surface of the plug body. The bottom surface of the plug body is the end surface of the solid insertion protrusion of the plug body, or the surface on the opposite side of the top surface of the disk-shaped plug top wall where the insertion protrusion is not formed. It is an inner side part from the contact surface of an outer peripheral part.

  The screwing wall portion of the stopper of the stopper with a collection piece is not limited to a cylindrical configuration centered on the stopper central axis. The screwing wall portion may have a configuration in which a cross-sectional shape perpendicular to a plug central axis on the outer periphery thereof is a polygonal shape such as a rectangle or a hexagon.

The stopper with the collection piece is not limited to a configuration having a screwing wall part that can be screwed to the outer periphery of the opening end of the container tube wall part 22.
The stopper with the collection piece does not have a screwing wall part that can be screwed to the outer periphery of the opening end of the container tube wall part 22, and can be inserted into the opening end part of the container tube wall part 22 to be detachable from the container tube wall part 22. It is also possible to employ a configuration having a plug body in which a portion to be attached (hereinafter also referred to as an insertion attachment portion) is secured. The plug body may have a configuration having an insertion mounting portion that can be screwed to the inner periphery of the opening end portion of the container cylinder wall portion 22 or a configuration having an insertion mounting portion that can be fitted to the opening end portion of the container cylinder wall portion 22. .
The bottom surface of the plug body having the insertion mounting portion is a surface facing the inner space of the container when the insertion mounting portion is inserted into the opening end of the container cylinder wall portion 22. The plug body having no insertion wall portion and having an insertion mounting portion has a plug body top surface on the side opposite to the bottom surface of the plug body. The sampling projection of the stopper with a sampling piece that does not have a threaded wall and has an insertion mounting portion extends from the outer periphery of the bottom surface of the stopper secured to the insertion mounting portion to the side opposite to the top surface of the plug body. .

  DESCRIPTION OF SYMBOLS 10 ... Sample collection tool, 20 ... Container, 21 ... Opening part of (container), 22 ... Cylindrical wall part of (container), 22a ... External thread part, 23 ... Bottom wall, 30 ... Plug body with collection piece, 31 ... Plug Body, 31L ... center axis of (plug body), 32 ... sampling piece, 32a ... support piece portion, 32b ... collection piece portion, 33 ... annular top wall portion, 33a ... contact surface, 33b ... top surface of plug body 34 ... (plug body) insertion protrusion, 34a (cylindrical side wall) 34b ... plug body bottom wall part, 34c ... plug body recess (inside space), 34d ... (plug body) Bottom surface (bottom surface), 35... Screwing wall portion, 35 a... Inner thread portion, 41.

Claims (6)

A container having an opening formed on the other end side opposite to the bottom wall on one end side, and a plastic projecting piece for collecting a sample from a stopper attached to the other end side of the container . A stopper with a collection piece,
The stopper is capable of sealing the opening of the container , and has an insertion protrusion that is inserted into the opening when the opening of the container is sealed. A cylindrical side wall portion projecting from the inner peripheral portion of the annular top plate portion in contact with the peripheral edge portion of the opening of the container to one side in the axial direction thereof, and the cylindrical side wall of the inner space of the cylindrical side wall portion A plug bottom wall that closes the protruding end side and forms the bottom surface of the plug,
The harvesting protrusion has taken piece extending from the tip of the support piece projecting from the outer peripheral portion of the outer peripheral portion or the bottom surface of the bottom surface of the plug member, the collecting piece, the plug body Through the center axis of the plug body with respect to the central axis of the plug body that coincides with the central axis of the opening of the container when the opening of the container is sealed from the bottom surface or the tip of the support piece It is inclined and extended to the opposite side ,
The collection projecting piece is entirely the collection piece part, or the extension dimension from the support piece part and the support piece part is the projecting dimension of the support piece part from the bottom surface of the plug body. A sampling tool characterized in that the sampling piece portion is larger than the sampling piece portion .   The sample collection tool according to claim 1, wherein a tip of the collection piece portion is located on an extension of a central portion of the bottom surface in a central axis direction of the plug body.   The base end portion on the plug body side of the sampling piece portion is formed in an arc plate shape extending in a curved cross-sectional shape along the outer peripheral portion of the bottom surface of the plug body. The sampling tool as described. The sampling tool according to any one of claims 1 to 3 , wherein the stopper is formed with a recess into which a finger can be inserted from a side opposite to the bottom surface. A sample collection method comprising collecting a sample using the sample collection tool according to any one of claims 1 to 4 . A sample inspection method, comprising: collecting a sample by the sample collection method according to claim 5; and subjecting the collected sample to inspection.

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