JP6347006B1 - Seamless capsule and method for producing the same - Google Patents

Abstract

A seamless capsule provided with a capsule film, wherein the capsule film contains a galactose partial decomposition product of galactoxyloglucan and polyphenol.

Description

  The present invention relates to a seamless capsule and a method for producing the same.

Conventionally, capsules have been used as orally used products such as various pharmaceuticals and foods.
Seamless capsules are used as such capsules. Such a seamless capsule is provided with a capsule film, and the capsule film functions as a container for containing contents such as active ingredients in the inside or the film itself.

As this type of seamless capsule, a seamless capsule in which the capsule film contains sodium alginate and a calcium salt has been proposed (see Patent Document 1).
In addition, a seamless capsule in which the capsule film has a multi-layered structure film has been proposed (see Patent Document 2).

  On the other hand, by containing a galactose partial degradation product of galactoxyloglucan and polyphenol, a composition capable of exhibiting thickening and gelling action has been proposed (see Patent Document 3).

Japanese Unexamined Patent Publication No. 1-331421 Japanese Unexamined Patent Publication No. 2006-232760 Japanese Unexamined Patent Publication No. 2000-354460

  However, in the technique of Patent Document 1, a calcium salt, that is, a metal ion is required to form a capsule film. Therefore, it becomes difficult to add a component that reacts with metal ions as the content, and as a result, the type of the content may be limited. In addition, if the content contains metal ions, the capsule film is insolubilized accordingly, and as a result, the capsule film may be unnecessarily delayed in dissolution or elution.

In addition, in the technique of Patent Document 1, it is necessary to form a sodium alginate in a capsule shape and then cross-link the surface with calcium. Therefore, high technical ability and special equipment are required, and the production is complicated. There is a risk.
Further, in the technique of Patent Document 2, it is necessary to form a plurality of constituent films, and accordingly, high technical ability and special equipment are required, and the production may be complicated.

  On the other hand, Patent Document 3 does not disclose that a capsule film of a seamless capsule can be formed by using a galactose partial decomposition product of galactoxyloglucan and polyphenol.

  In view of the above circumstances, an object of the present invention is to provide a seamless capsule that can be filled with a wider range of contents than before and that can be easily manufactured, and a method for manufacturing the same.

The present inventors diligently studied the reactivity of a galactose partial degradation product of galactoxyloglucan (hereinafter sometimes simply referred to as “galactose partial degradation product”) with polyphenol.
Surprisingly, when an aqueous solution of a partial decomposition product of galactose is dropped into an aqueous solution of polyphenol, the surface of the droplet of the aqueous solution of partial decomposition product of galactose reacts instantaneously with polyphenol, resulting in gelation, which causes Was found to be covered with a film. That is, it has been found that a capsule film of a seamless capsule can be easily formed.

  Moreover, it discovered that the thickness of the capsule film | membrane changed according to the time which hold | maintains the droplet of galactose partial decomposition product aqueous solution in polyphenol aqueous solution, and the density | concentration of polyphenol aqueous solution. Further, the present invention is completed by finding that the polyphenol penetrates more into the inside of the droplet depending on the time and concentration, and that the entire droplet can finally be gelled. It came.

That is, the seamless capsule according to the present invention is
A seamless capsule with a capsule coating,
The capsule film contains a galactose partial decomposition product of galactoxyloglucan and polyphenol.

  According to such a configuration, the capsule film contains a galactose partial decomposition product and polyphenol, so that it becomes a seamless capsule that can be filled with a wider range of contents than ever and can be easily manufactured.

In the seamless capsule of the above configuration,
It is preferable that the galactose partial degradation product is obtained by decomposing 30 to 55% of galactose.

According to such a configuration, the galactose partial decomposition product is obtained by decomposing 30 to 55% of galactose, whereby the reactivity with polyphenol is improved, and thus a relatively uniform film can be formed in a relatively short time.
Therefore, a seamless capsule having a capsule film with higher strength can be produced in a shorter time.

In the seamless capsule of the above configuration,
It is preferable that the polyphenol is a tea extract.

  According to this configuration, since the polyphenol is a tea extract, the reactivity with the partially decomposed product of galactose is improved, so that a seamless capsule having a capsule film with higher strength can be produced in a shorter time.

The method for producing a seamless capsule according to the present invention includes:
Capsules containing the galactose partial decomposed product and the polyphenol by dropping a first aqueous solution containing a galactose partial decomposed product of galactoxyloglucan and water into a second aqueous solution containing polyphenol and water. It is a method provided with the process of forming the seamless capsule which has a membrane | film | coat.

According to this configuration, a capsule capsule can be formed simply by dropping the first aqueous solution into the second aqueous solution, so that seamless capsules can be easily produced.
In addition, as described above, the obtained seamless capsule can accommodate a wider range of contents than before.
Therefore, a seamless capsule that can be filled with a wider range of contents than before can be easily produced.

In the method for producing a seamless capsule having the above-described configuration,
The concentration of the galactose partial decomposition product in the first aqueous solution is preferably 0.5 to 3.0% by mass.

  According to such a configuration, by setting the concentration of the galactose partial decomposition product in the first aqueous solution to 0.5 to 3.0% by mass, the first aqueous solution has a viscosity suitable for dropping, and the galactose partial decomposition product. Therefore, a seamless capsule with higher strength can be easily produced in a shorter time.

In the method for producing a seamless capsule having the above-described configuration,
The concentration of the polyphenol in the second aqueous solution is preferably 0.25 to 10% by mass.

  According to this structure, since the reactivity of a galactose partial decomposition product and polyphenol can be improved by making the density | concentration of the polyphenol in a 2nd aqueous solution into 0.25-10 mass%, higher intensity | strength seamless Capsules can be manufactured in a shorter time.

In the method for producing a seamless capsule having the above-described configuration,
It is preferable to use a tea extract as the polyphenol.

  According to such a configuration, by using the tea extract as the polyphenol, the reactivity between the partially decomposed galactose and the polyphenol is improved, so that a seamless capsule with higher strength can be produced in a shorter time.

The photograph of the seamless capsule which has each shape of one embodiment of the present invention Photograph showing an example of the maximum diameter of each shape seamless capsule of this embodiment

  Hereinafter, embodiments of the seamless capsule and the method for producing the same according to the present invention will be described.

  The seamless capsule of the present embodiment is a seamless capsule provided with a capsule film, and the capsule film contains a galactose partial decomposition product of galactoxyloglucan and polyphenol.

The galactoxyloglucan is a constituent component of the cell wall (primary wall) of higher plants such as dicotyledonous and monocotyledonous plants, and is a nonionic polymer polysaccharide that exists as a storage polysaccharide for some plant seeds. is there.
This galactoxyloglucan has glucose, xylose and galactose as constituent sugars, has β-1,4-linked glucose as a main chain, has xylose as a side chain, and further binds to the xylose Galactose.
The galactoxyloglucan may be any plant-derived galactoxyloglucan, and can be obtained from, for example, tamarind, jatoba, nastathium seed, soybean, mung bean, kidney bean, rice, barley, or other fruit epidermis. Considering that it is most easily available and has a large content, galactoxyloglucan derived from legume tamarind seeds is preferable. As such galactoxyloglucan, a commercially available product can be adopted. Examples of commercially available products include Griloid (registered trademark, manufactured by DSP Gokyo Food & Chemical Co., Ltd.).

The galactose partial degradation product of galactoxyloglucan is a substance obtained by partially decomposing and removing the side chain galactose of galactoxyloglucan.
In addition, in this embodiment, galactoxyloglucan means galactoxyloglucan (complete galactoxyloglucan) from which side chain galactose has not been removed by partial decomposition by enzyme treatment described later. Such complete galactoxyloglucan may also be referred to as native galactoxyloglucan.
An enzyme is used for the partial decomposition. Examples of the enzyme include β-galactosidase.

  The β-galactosidase is an enzyme that releases galactose by hydrolyzing the bond between galactose and xylose contained in galactoxyloglucan. β-galactosidase may be either plant-derived or microorganism-derived, but an enzyme derived from the microorganism Aspergillus oryzae or Bacillus circulans or an enzyme in seeds containing galactoxyloglucan is preferable. As such β-galactosidase, a commercially available product can be adopted.

  In this enzymatic reaction with β-galactosidase, side chain galactose is partially removed as the reaction proceeds, and when the removal rate reaches around 30%, the reaction solution rapidly thickens and gels. When the removal rate of galactose is in the range of 30 to 55%, the gel has a reversible thermal response gelation property that gels by heating and sols by cooling. If the galactose removal rate is less than 30%, the reactivity with polyphenol is reduced, and if it exceeds 55%, the solubility of the galactose partial degradation product in water is reduced, making it difficult to prepare a uniform aqueous solution of galactose partial degradation product, It tends to form a non-uniform film.

  Considering this point, it is preferable to use the above-mentioned partial decomposition product of galactose obtained by partial decomposition of 30 to 55% of galactose. When the galactose partial decomposition product is partially decomposed by 30 to 55% of galactose and reacted with polyphenol, a relatively uniform film can be formed in a relatively short time. Therefore, a seamless capsule having a capsule film with higher strength can be produced in a shorter time.

Galactoxyloglucan usually contains about 37% side chain xylose and about 17% side chain galactose (see Gidley et al., Carbohydrate Research, 214 (1991) 219-314). Therefore, it is calculated that the galactose partial decomposition product obtained by partially decomposing 30 to 55% of galactose contains 39 to 41% of side chain xylose and 8 to 12% of side chain galactose.
In addition, the partial decomposition rate of galactose (that is, the removal rate of galactose) is determined by measuring the amount of galactoxyloglucan oligosaccharide produced by cellulase decomposition of the obtained partial decomposition product using high performance liquid chromatography (hereinafter referred to as HPLC). It can be calculated by measuring with (amino column).

Examples of the polyphenol include chemically synthesized polyphenols and plant-derived polyphenols (plant polyphenols). Among these, plant polyphenols are preferred.
Plant polyphenols are green tea (matcha sencha, etc.), black tea, oolong tea, puer tea (black tea), mate tea and other tea leaves, white vegetables, citrus peels and seeds, grape peels and seeds (red wine, red grape juice, etc.) , Apples, strawberries, peaches, none, onion peels, chestnuts, burdock, coffee beans, cacao beans, and the like (including solid and liquid extracts). This extract is rich in monomeric polyphenols and polymer polyphenols.

Examples of the monomer polyphenol include flavonoids, chlorogenic acid, gallic acid, and ellagic acid.
Examples of the flavonoids include catechins, quercetin, rutin, and anthocyanins.
Examples of the catechins include (+)-catechin, (+)-gallocatechin, (−)-epicatechin, (−)-epigallocatechin, and gallic acid esters thereof. Among these, as the catechins, gallic acid ester (epigallocatechin gallate) of (−)-epigallocatechin is preferable.

Examples of the polymer polyphenol include condensed and hydrolyzed tannins.
Examples of the condensed tannin include proanthocyanidins and the like, and examples of the proanthocyanidins include thealvidin and prodelphinidin.
Examples of the hydrolyzable tannin include tannic acid, gallotannin, and ellagitannin.

When the above-mentioned plant polyphenol is used, the above-mentioned extract itself may be used, or a polyphenol which is an active ingredient may be concentrated (extracted) from the extract, and further the extract The active ingredient polyphenols may be purified (separated). Of these extracts, tea extract, which is an extract extracted from tea leaves, is preferable.
In addition, the said plant polyphenol is not specifically limited to said thing, As long as it is obtained from the plant containing polyphenol, any may be sufficient.
In addition, polyphenol may be used as a 1 type, or multiple types of mixture.

The capsule film contains a galactose partial decomposition product, polyphenol, and water.
Such a capsule film may be in the form of a gel or a film. A gel-like capsule film is obtained by mixing a partially decomposed galactose product, polyphenol, and water, and a film-like capsule film is obtained by reducing the water content of the obtained mixture by drying or the like.

  The content of the galactose partial decomposition product in the capsule film may be appropriately set according to the desired strength of the capsule film, and is not particularly limited.

  The content of polyphenol in the capsule film is appropriately set according to the degree to which the galactose partial degradation product can gel and the degree of gelation in the thickness direction of the capsule film, that is, according to the desired capsule film thickness. There is no particular limitation as long as it is sufficient.

  The size of the seamless capsule (that is, the size of the capsule film) is not particularly limited. For example, as shown in FIG. 2, the seamless capsule passes through the center of the seamless capsule, and both ends of the seamless capsule (virtual straight lines passing through the center are seamless capsules. The maximum length (maximum diameter) that connects the two intersections intersecting the surface of the surface may be 2 to 10 mm. The maximum diameter corresponds to the diameter when the seamless capsule is spherical. In the case of a teardrop shape, this corresponds to the maximum diameter of the spherical portion.

The thickness of the capsule film is not particularly limited, but is preferably about 3 to 40% of the maximum diameter, for example. The thickness ratio can be measured as a coating rate by the method described in Examples described later.
When the thickness of the capsule film is 3% or more, the film has a sufficient strength, and when it is 40% or less, a volume capable of accommodating other components inside the film can be secured.
In addition, even if it has the part which is not gelatinized inside a membrane | film | coat, the capsule membrane | film | coat may be in the solid state from which the whole surface is gelatinized from the surface of a membrane | film | coat. When the capsule film has a portion not gelled on the inside, an aqueous solution of a partially decomposed galactose solution is usually present on the inside.

  The capsule film of this embodiment may contain various additives that are usually added to the capsule film as necessary. Examples of such additives include plasticizers, preservatives, water activity lowering agents, pH adjusters and the like. Examples of the plasticizer include glycerin, sorbitol, propylene glycol, polyethylene glycol and the like. In addition to the above, additives for coating include excipients such as various polysaccharides such as various starches (including modified starch, modified starch, starch, starch degradation products, etc.). Is mentioned.

In the seamless capsule of the present embodiment, the contents can be accommodated in the capsule film (that is, in the aqueous solution of a partially decomposed galactose product) or in the capsule film (its own).
The contents are not particularly limited. Examples of the contents include oil agents such as olive oil and canola oil. Moreover, the thing etc. by which the active ingredient was disperse | distributed or melt | dissolved in this oil agent are mentioned. In addition, before dripping at the polyphenol aqueous solution, the said oil agent may be mixed and emulsified with the galactose partial decomposition product, and may be dripped at the polyphenol aqueous solution with the galactose partial decomposition product in this state. In this case, the oil agent is contained in the capsule film and the capsule film in an emulsified state with the aqueous solution of a partially decomposed galactose solution.
The ratio of the volume of the content to the volume of the entire seamless capsule is preferably 20 to 93% by volume. In this case, it is preferable that the coating rate of the capsule coating is 3 to 40%.

  Next, a method for producing the seamless capsule of this embodiment will be described.

The manufacturing method of the seamless capsule of this embodiment is as follows:
Capsules containing the galactose partial decomposed product and the polyphenol by dropping a first aqueous solution containing a galactose partial decomposed product of galactoxyloglucan and water into a second aqueous solution containing polyphenol and water. It is a method provided with the process of forming the seamless capsule which has a membrane | film | coat.

  Specifically, in the method for producing a seamless capsule of the present embodiment, a first aqueous solution is prepared by dissolving a galactose partial decomposition product in water, a second aqueous solution is prepared by dissolving polyphenol in water, The aqueous solution of 1 is dropped into the second aqueous solution. When the droplet of the first aqueous solution comes into contact with the second aqueous solution, it gels from the surface side of the droplet from the moment of dropping, and the second aqueous solution penetrates into the droplet as time passes, Gelation proceeds from the surface side toward the center. As a result, a capsule film is formed.

The density | concentration of the galactose partial decomposition product in 1st aqueous solution is not specifically limited, It can set suitably so that a capsule film | membrane may be formed. For example, the higher the concentration of the partially decomposed galactose, the higher the reactivity with polyphenol. On the other hand, if the concentration is too high, the viscosity tends to be excessively high and dripping tends to be difficult.
Therefore, for example, considering such a viewpoint, the concentration of the galactose partial decomposition product in the first aqueous solution is preferably 0.25 to 5.0% by mass, and preferably 0.5 to 3.0% by mass. Is more preferable.
By setting the concentration of the galactose partial decomposition product in the first aqueous solution to 0.25 to 5.0% by mass, the first aqueous solution has a viscosity suitable for dripping, and the reactivity between the galactose partial decomposition product and polyphenols. Therefore, a seamless capsule with higher strength can be easily produced in a shorter time.

The concentration of polyphenol in the second aqueous solution is not particularly limited, and can be appropriately set so that a capsule film can be formed. For example, the higher the concentration of polyphenol, the higher the reactivity with the partially decomposed product of galactose. On the other hand, when the concentration is too high, bitterness tends to be felt when the formed seamless capsule is contained in the oral cavity. .
Therefore, for example, in view of such a viewpoint, the concentration of the polyphenol in the second aqueous solution is preferably 0.1 to 20% by mass, and more preferably 0.25 to 10% by mass.
By setting the concentration of polyphenol in the second aqueous solution to 0.25 to 10% by mass, the reactivity between the partially decomposed galactose and polyphenol can be improved. Can be manufactured with. Moreover, the occurrence of bitterness when taken can be suppressed.

The temperature of the 1st aqueous solution at the time of dripping and the 2nd aqueous solution is not specifically limited, It can set suitably. For example, when the temperature of these aqueous solutions is high at the time of dripping, it tends to be difficult to form a capsule film.
Therefore, for example, considering this viewpoint, the temperature of the first aqueous solution and the second aqueous solution at the time of dropping is preferably 5 to 25 ° C.

The holding time of the 1st aqueous solution in the 2nd solution after dripping is not specifically limited, It can set suitably. For example, as the holding time becomes longer, polyphenol penetrates into the droplets and gels, and as a result, the coating tends to be thicker. Moreover, when holding time becomes still longer, the whole capsule film tends to be in a gelled state.
Therefore, for example, considering this viewpoint, it is preferable that the retention time after dropping is 10 seconds to 2 hours.
The supply of the second aqueous solution to the droplet is stopped by removing the droplet from the second aqueous solution. When the second aqueous solution that has not reacted (not yet involved in gelation) remains in the droplets, even after removal, the polyphenol diffuses into the interior, and gelation occurs. On the other hand, if the second aqueous solution can no longer participate in the reaction, gelation stops.

  The method of dripping the first liquid into the second liquid is not particularly limited, and a conventionally known dripping method can be adopted. Examples of the dropping method include a method using a cylindrical member having an opening at the tip. Examples of such a cylindrical member include a nozzle and a pipette.

  Moreover, the method of dripping in a 2nd liquid is also mentioned, accommodating the 3rd liquid different from this 1st liquid and a 2nd liquid in the inside of a 1st liquid. As such a method, for example, by using a double nozzle in which an inner nozzle is arranged inside an outer nozzle, the first liquid from the outer nozzle, the third liquid from the inner nozzle, Dropping into the second liquid may be mentioned so that the third liquid droplet is accommodated in the liquid droplet. In addition, by using two nozzles in which the other nozzle having a smaller diameter than the one nozzle is arranged outside the one nozzle, the first liquid droplet is discharged from the one nozzle. The third liquid droplet may be dropped into the second liquid so that the third liquid droplet is accommodated in the first liquid droplet.

Two or more types of droplets may be accommodated in the first droplet.
Alternatively, a capsule that is smaller than the droplets is dispersed in the first liquid, and this dispersion is dropped into the second liquid to produce a seamless capsule in which the capsule is accommodated inside the capsule film. Good.

  The size (maximum diameter) of the seamless capsule can be changed, for example, by changing the diameter (diameter) of the tip of the cylindrical member.

As described above, the seamless capsule of this embodiment is
A seamless capsule with a capsule coating,
The capsule film contains a galactose partial decomposition product of galactoxyloglucan and polyphenol.

  According to the seamless capsule of the present embodiment, the capsule film contains a galactose partial degradation product and polyphenol, so that it becomes a seamless capsule that can be filled with a wider range of contents than before and can be easily manufactured.

In addition, the seamless capsule of this embodiment has acid resistance because the capsule film contains a galactose partial decomposition product and polyphenol.
Furthermore, the seamless capsule of this embodiment can be decomposed by cellulase (cellulase degradability) because the capsule film contains a galactose partial decomposition product and polyphenol.
Therefore, the seamless capsule of this embodiment is suitable for applications where acid resistance is required and applications where cellulase degradability is required.
In particular, since it has both acid resistance and cellulase degradability, the seamless capsule of this embodiment is useful as an enteric seamless capsule.
Moreover, the seamless capsule of this embodiment can also exhibit the effect derived from polyphenol by having polyphenol. For example, it can exhibit antibacterial properties.

In the seamless capsule of this embodiment,
It is preferable that the galactose partial degradation product is obtained by decomposing 30 to 55% of galactose.
According to such a configuration, the galactose partial decomposition product is obtained by decomposing 30 to 55% of galactose, whereby the reactivity with polyphenol is improved, and thus a relatively uniform film can be formed in a relatively short time.
Therefore, a seamless capsule having a capsule film with higher strength can be produced in a shorter time.

In the seamless capsule of this embodiment,
It is preferable that the polyphenol is a tea extract.
According to this configuration, since the polyphenol is a tea extract, the reactivity with the partially decomposed product of galactose is improved, so that a seamless capsule having a capsule film with higher strength can be produced in a shorter time.

The manufacturing method of the seamless capsule of this embodiment is as follows:
Capsules containing the galactose partial decomposed product and the polyphenol by dropping a first aqueous solution containing a galactose partial decomposed product of galactoxyloglucan and water into a second aqueous solution containing polyphenol and water. It is a method provided with the process of forming the seamless capsule which has a membrane | film | coat.

According to the method for producing a seamless capsule of the present embodiment, a capsule film can be formed simply by dropping the first aqueous solution into the second aqueous solution, so that the seamless capsule can be produced easily.
In addition, as described above, the obtained seamless capsule can accommodate a wider range of contents than before.
Therefore, a seamless capsule that can be filled with a wider range of contents than before can be easily produced.

In the manufacturing method of the seamless capsule of this embodiment,
The concentration of the galactose partial decomposition product in the first aqueous solution is preferably 0.5 to 3.0% by mass.
According to such a configuration, by setting the concentration of the galactose partial decomposition product in the first aqueous solution to 0.5 to 3.0% by mass, the first aqueous solution has a viscosity suitable for dropping, and the galactose partial decomposition product. Therefore, a seamless capsule with higher strength can be easily produced in a shorter time.

In the manufacturing method of the seamless capsule of this embodiment,
The concentration of the polyphenol in the second aqueous solution is preferably 0.25 to 10% by mass.
According to this structure, since the reactivity of a galactose partial decomposition product and polyphenol can be improved by making the density | concentration of the polyphenol in a 2nd aqueous solution into 0.25-10 mass%, higher intensity | strength seamless Capsules can be manufactured in a shorter time.

In the manufacturing method of the seamless capsule of this embodiment,
It is preferable to use a tea extract as the polyphenol.
According to such a configuration, by using the tea extract as the polyphenol, the reactivity between the partially decomposed galactose and the polyphenol is improved, so that a seamless capsule with higher strength can be produced in a shorter time.

  As described above, according to the present embodiment, a seamless capsule that can be filled with a wider range of contents than conventional ones and that can be easily manufactured and a method for manufacturing the same are provided.

  The seamless capsule of the present embodiment has excellent acid resistance and is decomposed by cellulase derived from intestinal bacteria. Therefore, the seamless capsule can be widely used in foods, pharmaceuticals, cosmetics, industrial applications, and particularly used in foods. It is useful. Furthermore, when encapsulating lactic acid bacteria in a capsule, the lactic acid bacteria are protected from the gastric juice by the capsule film showing acid resistance, and after moving into the intestine, the capsules are decomposed by the enzyme, and the lactic acid bacteria are released. Is useful in that it can be used as an enteric capsule that is delivered to the intestine without being killed and then dissolved in the intestine.

  Although the present embodiment has been described above, the present invention is not particularly limited to the above-described embodiment, and can be appropriately modified within the scope intended by the present invention.

  Hereinafter, although this invention is demonstrated in detail, referring an Example, this invention is not limited to these Examples.

(Production Example 1) Production of partial decomposition product of galactose 1 g of galactoxyloglucan (manufactured by DSP Gokyo Food & Chemical Co., Ltd., Griloid (registered trademark)) is added to 99 g of water, and stirred at 75 ° C. for 15 minutes. A 1% by mass galactoxyloglucan aqueous solution containing 1% by mass was obtained. By using a purified enzyme β-galactosidase, a 1% by mass galactoxyloglucan aqueous solution is reacted at an enzyme concentration of 2.4 × 10 ⁻⁵ % by mass, pH 5.6, 50 ° C., and then heated at 100 ° C. for 20 minutes. The reaction was stopped. The reaction solution gelled about 15 hours after the start of the reaction, thereby obtaining a gel-like composition. The galactose removal rate in the obtained gel composition was calculated by the following method.

  1 mL of a cellulase Onozuka RS (Yakult Pharmaceutical Co., Ltd.) 0.15% by weight (50 mM acetate buffer, pH 4.0) was added to 7 g of a gel-like composition (1% by weight aqueous solution) at 50 ° C. overnight. It was made to react with. The 1 mass% galactoxyloglucan aqueous solution prepared above was reacted in the same manner as a control. After the reaction, the reaction solution was heated at 98 ° C. for 30 minutes to inactivate the enzyme to obtain a sample. Thereafter, the obtained sample was applied to a pretreatment cartridge (manufactured by Tosoh Corporation, IC-SP) and a 0.45 μm cellulose acetate membrane filter, and 10 μL of the obtained filtrate was acetonitrile: water = 60: 40 (v / v) was applied to an HPLC amino column flowing at 0.6 mL / min, and oligosaccharides of galactoxyloglucan (7 sugars (0 galactose), 8 sugars (1 galactose), 9 sugars (2 galactoses) ) Was detected with a differential refractometer. Next, the amount of galactose per unit (7 sugars) was calculated by (8 sugar area + (9 sugar area × 2)) / (7 sugar area + 8 sugar area + 9 sugar area). When the reduction rate from the galactose amount calculated for the control galactoxyloglucan was further calculated as the galactose removal rate (%) of the galactose amount calculated for the gel composition using the above formula, it was about 45%. there were.

  The gel-like composition obtained above was blown and dried, then pulverized and sieved to obtain a powdered galactose partial decomposition product.

(Test Example 1)
<Relationship between the concentration of galactose partial degradation products and the concentration of polyphenol>

(Preparation of galactose partial decomposition product aqueous solution (first aqueous solution))
Add 195.0 g of deionized water to a 300 mL stainless beaker, add 5.0 g of the partially decomposed galactose obtained in Production Example 1 and mix at room temperature to obtain a 2.50 mass% dispersion of the partially decomposed galactose. Prepared. The dispersion was allowed to stand at −20 ° C. for 2 hours, and then allowed to stand at room temperature for 30 minutes to thaw, and then the thawed product was stirred with a stirrer for 1 hour to homogenize. The obtained 2.50 mass% aqueous solution of the partially decomposed galactose was diluted with deionized water to obtain 0.25, 0.50, 0.75, 1.00, 1.25, 1.50, 2.00. A 2.50 mass% aqueous solution of a partially decomposed galactose product was prepared.

(Preparation of galactoxyloglucan aqueous solution (comparative example))
As a comparative example, 197.5 g of deionized water was placed in a 300 mL stainless beaker, and 2.5 g of galactoxyloglucan (galactoxyloglucan in which galactose was not partially decomposed) was added and stirred at room temperature for 1 hour. A 1.25% by mass aqueous solution of xyloglucan was prepared.

(Preparation of polyphenol aqueous solution (second aqueous solution))
Add 97.5 g of deionized water to a glass beaker with a capacity of 200 mL, add 2.5 g of Sanphenon 90-S (tea extract, containing 70% or more of catechins, Taiyo Kagaku Co., Ltd.) as a polyphenol, and mix. A 90-S 2.50 mass% aqueous solution was prepared. The obtained Sanphenon 90-S 2.50 mass% aqueous solution was diluted with deionized water to obtain 0.25, 0.50, 0.75, 1.00, 1.25, 1.50, 2.00, 2. A 50 mass% aqueous solution was prepared.

10 g of each concentration of Sanphenon 90-S aqueous solution was put into a glass beaker with a capacity of 10 mL, and each concentration of galactose partial degradation product aqueous solution and galactoxyloglucan aqueous solution was added dropwise with a dropper (caliber 1 mm). One to two minutes after dropping, the state of the dropped galactose partial decomposition product aqueous solution and galactoxyloglucan aqueous solution is observed with the naked eye, and it is evaluated whether or not a seamless capsule is formed. The shape was evaluated according to the following criteria.
In addition, when a seamless capsule is formed, the obtained seamless capsule is collected, washed with water, and its strength (breaking stress) is measured with a creep meter (model: RE2-30005S, manufactured by Yamaden Co., Ltd.). And measured. (40 mm plunger, 1 mm / sec speed, unit: Pa). The measurement was performed three times, and the average value of the obtained measurement values was defined as the strength of the seamless capsule.
The results are shown in Table 1. The shape of the seamless capsule is shown in FIG.

(Criteria)
-Judgment criteria for whether or not seamless capsules are formed (formability) ○: Seamless capsules are formed, and the strength of the capsules can be crushed with a spoonful and used for the measurement of strength by the creep meter. it was high.
Δ: A seamless capsule was formed, but the film was broken when scooped up with a spoon. That is, the strength was so low that it could not be used for measuring the strength by the creep meter.
X: Seamless capsules were not formed.

・ Criteria for seamless capsule shape Sphere: Spherical seamless capsule Tear: Tears-shaped seamless capsule Strain sphere: Spherical but distorted seamless capsule Flat: Seamless capsule with flat shape

As shown in Table 1, it was found that seamless capsules could be formed when the concentration of the partially decomposed galactose was 0.50% by mass or more. When the concentration is 0.75% by mass or more, a seamless capsule having a strength that can be scooped up with a spoon is formed, and when the concentration is 1.00 to 1.50% by mass or more, It was found that spherical seamless capsules having such a strength that can be scooped up can be formed.
On the other hand, it was found that if the polyphenol concentration is 0.25% by mass or more, seamless capsules can be formed. In addition, it was found that when the concentration is 0.50% by mass or more, spherical seamless capsules having such strength that can be scooped up with a spoonful can be formed.
As for the shape of the seamless capsule, when the concentration of the partially decomposed galactose product is a relatively low concentration of about 0.75% by mass, when the aqueous solution of the partially decomposed galactose solution is dropped, liquid droplets are dropped when falling into the polyphenol aqueous solution. Due to the impact force applied to the capsule film, the shape of the capsule film tends to be flat or distorted as described above. On the other hand, when the concentration is 1.00 to 1.50% by mass, the shape of the droplet tends to be maintained even when the impact force is applied due to sufficient viscosity (surface tension) and tends to be spherical. It was. Furthermore, when the concentration is higher than 2.00% by mass, the viscosity becomes too high, and the droplet is dropped while pulling and is fixed as it is, so that it tends to be a tear-shaped seamless capsule.

(Test Example 2)
<Influence of other polyphenol aqueous solution>

(Preparation of polyphenol aqueous solution (second aqueous solution))
The following polyphenol-containing drinking water was used as the polyphenol aqueous solution.
In other words, Sencha (trade name: “Oi Ocha” manufactured by ITO EN), Sencha (dark) (Product name: “OI Ocha Dark Tea” manufactured by ITO EN), Oolong Tea (trade name: “Oolong Tea” Suntory Ltd.) ), Black tea (trade name: “Afternoon Tea (Clear Straight)” Kirin Beverage Co., Ltd.) and coffee (trade name: “Premium Boss Black” Suntory Co., Ltd.), grape juice (trade name: “Welch”) Grape 100 "Asahi Beverage Co., Ltd.) was used.
Moreover, 0.25, 0.50, 1.00, 1.50 mass% aqueous solution of theabigo (contains 94% or more of epigallocatechin gallate, manufactured by Taiyo Kagaku Co., Ltd.) was also used as the polyphenol aqueous solution.
Furthermore, 0.25, 0.50, 1.00, 1.50 mass% aqueous solution of tannic acid (manufactured by Nacalai Tesque) was also used as the polyphenol aqueous solution.

10 g of polyphenol aqueous solution was put into a glass beaker having a capacity of 10 mL, and the galactose partial decomposition product aqueous solution and galactoxyloglucan aqueous solution of each concentration prepared in Test Example 1 were dropped into the polyphenol aqueous solution with a dropper (1 mm in diameter). After 1 to 2 minutes from dropping, the state of the dropped galactose partial decomposition product aqueous solution and galactoxyloglucan aqueous solution is observed with the naked eye, and using the above-mentioned determination criteria, whether or not a seamless capsule is formed is evaluated, When formed, the shape was evaluated according to the aforementioned criteria.
When seamless capsules were formed, their strength (unit: Pa) was evaluated by the measurement method described above.
The results are shown in Table 2.

As shown in Table 2, in the polyphenol-containing beverage, seamless capsules were not formed 1 to 2 minutes after dropping. This is presumably due to the low concentration of polyphenols.
However, when galactose partial decomposition product aqueous solution was dropped into Sencha (dark), black tea, and grape juice, and the droplets were held for 1 hour, seamless capsules were formed.
From this, it was found that seamless capsules can be produced by holding for a long time even when droplets of a partially decomposed galactose aqueous solution are dropped on a polyphenol-containing beverage (polyphenol aqueous solution having a relatively low concentration). .
In addition, when Teavigo (epigallocatechin gallate) is used, spherical seamless capsules having such strength that can be scooped up with a spoonful even with a low concentration solution of 0.25% by mass are formed. I knew I would get.
Furthermore, when tannic acid was used, spherical seamless capsules tended to be easily formed although the strength of the film was slightly weaker than that of Teabigo.

(Test Example 3)
<Gelization rate of capsule film>
A 1.25% by mass aqueous solution of the partially decomposed galactose prepared in Test Example 1 was dropped into a 1.25% by mass aqueous solution of Sanphenon 90-S with a dropper (1 mm in diameter). After dripping, confirm the state of the capsule film when it is allowed to stand for 10 minutes, 30 seconds, 1 minute, 5 minutes, 10 minutes, 30 minutes, 60 minutes, 120 minutes. The coating rate and strength were measured.
The results are shown in Table 3.

Criteria for determining the capsule film state A: A film is formed, and when pressed with a finger, it is crushed and comes out with liquid from the inside.
B: Although a film is formed, it is not easily crushed even when pressed with a finger (has high strength), and a small amount of liquid comes out from the inside.
C: A film is formed, gels up to the center, hardly crushes even when pressed with a finger (has higher strength), and no liquid comes out from the inside.

・ Measurement method of capsule film ratio (thickness index) Seamlessly immersed for 10 seconds, 30 seconds, 1, 5, 10, 30, 60, 120 minutes after dropping a partially decomposed galactose aqueous solution into a polyphenol aqueous solution Capsule is collected and bisected in the center with scissors, and the film is gently spread on a polyethylene / nylon film, and the film thickness (film thickness + film thickness) is measured with the digital outer micrometer. (Model: MCD130-25, manufactured by Niigata Seiki Co., Ltd.) Measurement was performed three times for each immersion time, an average value was calculated, and the thickness of the film was calculated by subtracting the film thickness (average value of the three times measurement value) from the average value.
Furthermore, after dripping as mentioned above and having passed for 10 minutes (it was immersed as it is for 10 minutes), a seamless capsule was taken out, the 10 largest thickness was measured using a digital outside micrometer, and the average value This average value was taken as the maximum diameter of the seamless capsule.
And the film rate was computed by the following formula | equation from the thickness of a film | membrane, and the maximum diameter of a seamless capsule.
Film rate (%) = {(film thickness) × 2 / (maximum diameter of seamless capsule)} × 100
In this test example, in the above-described measurement of the coating rate, the capsule coating after 10 minutes was spherical, and the maximum diameter (diameter) was 4.0 mm. Therefore, the coating rate was calculated using this maximum diameter.

As shown in Table 3, a capsule film was formed only by holding droplets of the partially decomposed galactose in an aqueous polyphenol solution for about 10 seconds. Since this capsule film is not crushed even when taken out from the polyphenol aqueous solution, it was found that it can be taken out and handled for various uses.
In addition, the longer the holding time, the thicker the film and the stronger the film.
Furthermore, when it hold | maintained for 60 to 120 minutes, it turned out that it can gelatinize to the center part of a droplet.
In addition, after dripping the galactose partial decomposition product aqueous solution to the polyphenol aqueous solution, each seamless capsule obtained by being immersed for 1 minute and 5 minutes was taken out and immersed in deionized water. Became almost 100%. From this, even after taking out from the polyphenol aqueous solution, it is presumed that the polyphenol remaining in the film permeated gradually toward the inside to form a gel, thereby thickening the film.

(Test Example 4)
<Seamless capsule size>
A 1.25% by mass aqueous solution of a galactose partially decomposed product of galactoxyloglucan was added dropwise to a 1.25% by mass aqueous solution of sunphenone 90-S 10 times with each instrument shown in Table 4 below. After each minute, each droplet was collected, washed with deionized water, and then accommodated in deionized water to obtain 10 seamless capsules each. The obtained seamless capsules were all spherical. For each instrument used, the maximum diameter of the obtained seamless capsule (capsule film) was measured by the digital outer micrometer, and the average value of 10 seamless capsules was calculated. As shown in FIG. 2, as the maximum diameter of the seamless capsule, the length indicated by the arrow in each shape of FIG. 2 was measured with the digital outer micrometer.
The results are shown in Table 4.

  As shown in Table 4, although the size of the seamless capsule obtained by the instrument used tends to be different, the seamless capsule is produced in the range of a maximum diameter of about 2.0 to 9.0 mm regardless of which instrument is used. It turns out that can be done.

(Test Example 5)
<Acid resistance of seamless capsules>
A 1.25% by mass aqueous solution of the galactose partial decomposition product of galactoxyloglucan was dropped into a 1.25% by mass aqueous solution of Sanphenon 90-S with a dropper (1 mm in diameter), collected 1 minute after the dropwise addition, and washed with deionized water. Then, it was accommodated in deionized water to obtain a seamless capsule.
The obtained seamless capsules are put into an aqueous solution having each pH (hydrochloric acid on the acidic side and sodium hydroxide added to deionized water on the alkaline side), 1, 5, 10, 30, 60, The state after 120, 180, 240 minutes and 1 day was visually observed, and the acid resistance was evaluated according to the following criteria.

・ Criteria for acid resistance ○: No change to seamless capsules.
Δ: The seamless capsule is dissolved and becomes smaller.
X: The seamless capsule dissolves and disappears.

  As shown in Table 5, the obtained seamless capsule maintained its shape and did not dissolve even when exposed to a strong acid having a pH of 1.0. On the other hand, it was dissolved in an alkaline solution having a pH of 11.0 or higher. As a result, it was found that the seamless capsule of this embodiment is excellent in acid resistance.

(Test Example 6)
<Acid resistance of seamless capsules containing gelatin in capsule film>
Gelatin (manufactured by Morinaga & Co., Ltd.) (0.3 g) was added to 9.7 g of deionized water and stirred while heating at 95 ° C. for 1 minute to dissolve the gelatin in deionized water. An aqueous gelatin solution was obtained. While maintaining the obtained gelatin aqueous solution at 40 ° C. or higher, it was dropped into silicone oil (KF-96-10CS, manufactured by Shin-Etsu Chemical Co., Ltd.) cooled to 5 ° C. or lower with ice water. Ten minutes after dropping, the drop was collected, and excess silicone oil adhering to the surface was gently wiped off with a Kim wipe to obtain a seamless capsule containing gelatin in the capsule film.

The obtained seamless capsule is put in a hydrochloric acid aqueous solution of pH 1.0 and tap water of pH 7.3, and 1, 5, 10, 30, 60, 120, 180, 240 minutes, the state after one day is visually observed. Observed.
As a result, in tap water, the seamless capsule maintained its shape and did not dissolve even after 1 day. On the other hand, in an aqueous solution of a strong acid having a pH of 1.0, when trying to scoop up with a spoon after 10 minutes, the seamless capsule was broken, and it was found that the seamless capsule was dissolved.
As a result, it was found that the seamless capsule containing the galactose partial degradation product and polyphenol in the capsule film had better acid resistance than the seamless capsule containing gelatin in the capsule film.

  Gelatin is an animal raw material and its use may be restricted due to BSE and religious problems, whereas galactose partial degradation products and polyphenols are plant raw materials, and such use restrictions are limited. Can be avoided. In addition, when gelatin is used, it is necessary to heat the gelatin in order to dissolve it in water. Therefore, it is difficult to accommodate contents with low thermal stability, and the selection range of contents is narrowed. When partially decomposed products and polyphenols are used, it is not necessary to heat them, so it is possible to accommodate contents with low thermal stability, and the range of selection of contents is wider than when gelatin is used. .

(Test Example 7)
<Dissolution of seamless capsules with cellulase>
A 1.25% by mass aqueous solution of the galactose partial decomposition product of galactoxyloglucan is dropped into a 1.25% by mass aqueous solution of Sanphenon 90-S with a dropper (caliber: 1 mm), and recovered after 1 minute from dropping, with deionized water. After washing, the capsules were accommodated in deionized water to obtain seamless capsules.
Cellulase Onozuka RS (manufactured by Yakult Pharmaceutical Co., Ltd.) 0.015% by mass aqueous solution (pH 4.0), the obtained seamless capsules are put into a water bath at room temperature or 40 ° C. The appearance after 5, 10, 30, 60, 120, 180, 240 minutes and 1 day was visually observed, and the solubility by cellulase was evaluated according to the following criteria.
The results are shown in Table 6.

-Criteria for solubility by cellulase ○: No change to seamless capsules.
Δ: The seamless capsule is dissolved and becomes smaller.
X: The seamless capsule dissolves and disappears.

As shown in Table 6, seamless capsules were dissolved by cellulase. Moreover, it melt | dissolved faster than the room temperature at the temperature of 40 degreeC.
Here, cellulase is known to be produced in the intestine by intestinal bacteria.
Therefore, as a result of the above, when a seamless capsule is taken, it is expected that the seamless capsule will be disintegrated and dissolved in the intestine by cellulase produced by intestinal bacteria.
Reference A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes, Nature. 2014 February 27; 506 (7489): 498-502 shows that human intestinal bacteria assimilate xyloglucans. ing.
Since the film of the seamless capsule of the present embodiment is formed by galactoxyloglucan derived from tamarind, that is, xyloglucans, it is sufficiently conceivable that the seamless capsule disintegrates and dissolves in human intestinal bacteria.

  As is clear from the above results, it was found that a seamless capsule that has acid resistance and that disintegrates and dissolves in the intestine can be obtained by a simple method of dropping a galactose partial decomposition product aqueous solution into a polyphenol aqueous solution. . Thus, it was found useful for enteric capsules.

(Test Example 8)
<Seamless capsule containing xanthan gum aqueous solution inside capsule film>
A 1.5% by mass aqueous solution of a galactose partial decomposition product of galactoxyloglucan, a 1.25% by mass aqueous solution of Sanphenon 90-S, and Keldent (xanthan gum, manufactured by DSP Gokyo Food & Chemical Co., Ltd.) 2.0% by mass An aqueous solution was used.
When the galactose partial decomposition product aqueous solution was dropped into the Sanphenon 90-S aqueous solution with a pipette (8 mm in diameter), the Keldent aqueous solution was dropped into the droplets of the galactose partial decomposition product aqueous solution with a dropper (1 mm in diameter). After dropping, the drop was collected after 4 hours, washed with deionized water, and then accommodated in deionized water to obtain seamless capsules.

The obtained seamless capsule was strong enough to be scooped up with a spoon, but when pressed with a finger, the capsule film was crushed and liquid came out from the inside. When the film ratio of the obtained seamless capsule was measured by the above-described measuring method, it was 18%.
Here, when only a 1.5% by mass aqueous solution of the galactose partial decomposition product was dropped into a 1.25% by mass aqueous solution of Sanphenon 90-S, the gelation had occurred to the center 4 hours after the dropping. Considering this, in the seamless capsule containing the Keldent aqueous solution, the part other than the part where the Keldent aqueous solution exists (the outer part where the galactose partial degradation product exists) gels, and the Keldent aqueous solution is in a sol state It was found that the capsule film was retained inside.
The viscosity of a 2.0 mass% aqueous solution of Keldent was measured with a B-type viscometer (model: TVB-25L, manufactured by Toki Sangyo Co., Ltd.) and found to be 3192 mPa · s (25 ° C., 30 rpm). The 2.0 mass% aqueous solution of was a liquid with a viscosity higher than water.

(Test Example 9)
<Seamless capsules containing glycerin in the capsule film>
In Test Example 8, concentrated glycerin for cosmetics (glycerin, manufactured by Kao Corporation) was used in place of the Keldent 2.0% by mass aqueous solution. Otherwise, seamless capsules were obtained in the same manner as in Test Example 8.
The obtained seamless capsule was strong enough to be scooped up with a spoon, but when pressed with a finger, the capsule film was crushed and liquid came out from the inside. The film rate of the obtained seamless capsules was measured by the above measurement method and found to be 37%.
As a result, as in the case of the Keldent aqueous solution of Test Example 8, it was found that glycerin (having a specific gravity greater than that of water) was retained inside the capsule film in a sol state.

(Test Example 10)
<Seamless capsules containing olive oil inside capsule film>
In Test Example 8, the galactose partial decomposition product aqueous solution was dropped with a dropper (4 mm diameter) instead of a pipette (caliber 8 mm), and a 2.0 mass% aqueous solution of Keldent was dropped into the droplets of the galactose partial decomposition product (1 mm diameter). The olive oil refined (manufactured by DSP Gokyo Food & Chemical Co., Ltd.) was injected with a Pasteur pipette (caliber 1 mm). Otherwise, seamless capsules were obtained in the same manner as in Test Example 8.
The obtained seamless capsule was strong enough to be scooped up with a spoon, but when pressed with a finger, the capsule film was crushed and liquid came out from the inside. The film rate of the obtained seamless capsules was 30% when measured by the above measuring method.
As a result, it was found that olive oil was retained inside the capsule film in a sol state as in the case of the Keldent aqueous solution of Test Example 8 and the glycerin of Test Example 9.

(Test Example 11)
<Seamless capsules containing emulsified olive oil inside capsule film>
15 g of olive oil refined (made by DSP Gokyo Food & Chemical Co., Ltd.) and 10 g of deionized water are added to 25 g of a 2.5% by weight aqueous solution of a galactose partial decomposition product of galactoxyloglucan, and a homogenizer (model: M2-f, Primix) is added. The mixture was stirred at 5000 rpm for 30 minutes and mixed to prepare an emulsion.
The obtained emulsion was dropped into a 1.25% by mass aqueous solution of Sanphenon 90-S with a dropper (caliber 1 mm). After dropping, the dropped product was recovered after 3 hours, washed with deionized water, and then accommodated in deionized water to obtain seamless capsules.
The obtained seamless capsules were strong enough to be scooped with a spoon. Moreover, even if it pressed with the finger | toe, the capsule membrane | film | coat was not crushed and the liquid did not come out from the inside. When the film ratio of the obtained seamless capsule was measured by the above measuring method, it was 100%.
As a result, it is considered that the entire droplet gels while the olive oil is uniformly dispersed, and the olive oil is held in the entire capsule film.

(Test Example 12)
<Seamless capsules containing rutin in the capsule film>
Add 2.5 g of rutin (manufactured by Nacalai Tesque Co., Ltd.) and 10 g of deionized water to 12.5 g of a 2.5% by weight aqueous solution of galactoxyloglucan galactose partial decomposition product, and stir with a stir bar to mix. A dispersion in which rutin was dispersed in the decomposition product aqueous solution was obtained.
The obtained dispersion was dropped into a 1.25 mass% aqueous solution of Sanphenon 90-S with a dropper (1 mm in diameter). After dripping, it collect | recovered after progress for 3 hours, and after washing | cleaning with deionized water, it accommodated in deionized water and obtained the seamless capsule.
The obtained seamless capsules were strong enough to be scooped with a spoon. Moreover, even if it pressed with the finger | toe, the capsule membrane | film | coat was not crushed and the liquid did not come out from the inside. When the film ratio of the obtained seamless capsule was measured by the above measuring method, it was 100%.
As a result, it is considered that the entire droplet is gelled while the solid rutin is uniformly dispersed, and the rutin is held in the entire capsule film.

(Test Example 13)
<Seamless capsules dried with olive oil contained inside the capsule membrane>
Concentrated glycerol 0.25 g and deionized water 12.25 g are added to 12.5 g of a 2.5% by mass aqueous solution of a galactoxyloglucan galactose partial decomposition product, and the mixture is stirred and mixed with a stir bar to obtain a galactose partial decomposition product 1.25 A mixed aqueous solution of mass% and glycerin 1.0 mass% was obtained.
When the obtained mixed aqueous solution was dropped into a 1.25 mass% aqueous solution of Sanphenon 90-S with a dropper (4 mm in diameter), olive oil refined (DSP Gokyo Food & Chemical Co., Ltd.) was placed inside the droplet of the mixed aqueous solution. The product was added dropwise with a Pasteur pipette (1 mm in diameter). After dropping, the drop was collected after 1 hour, and was left in an air dryer (model: SPH-201, manufactured by Espec Corp.) and dried at 50 ° C. for 2 hours to obtain a seamless capsule.

The obtained seamless capsule was strong enough to be scooped up with a spoon, but when pressed with a finger, the capsule film was crushed and liquid came out from the inside. This capsule film was film-like. When the film ratio of the obtained seamless capsule was measured by the above-described measuring method, it was 13%.
As a result, it was found that olive oil was retained inside the capsule film in the sol state as in Test Examples 10 and 11, even if the capsule film was in the form of a film.

As a result of Test Examples 8 to 13, it is possible to add a solution having a higher viscosity than water, a solution having a higher specific gravity, an oil agent, a water-insoluble powder, or the like as a content to the seamless capsule of the present embodiment. all right.
Moreover, as shown in Table 5 and Table 6 described above, when the seamless capsules of Test Examples 8 to 13 are decomposed by cellulase, it is assumed that each content is released to the outside.
Even when the galactose partial degradation product obtained by further purification of the galactose partial degradation product obtained in Production Example 1 was used, the same results as in Test Examples 1 to 5 and 7 to 13 were obtained. Does not affect the results.

Therefore, the seamless capsule and the method for producing the same of the present embodiment are effective for a seamless capsule that delivers lactic acid bacteria to the intestine while alive, a pharmaceutical, an animal drug, a supplement, and the like in which an active ingredient is released and exhibited in the intestine.
In addition, since the capsule film does not require the metal ions contained in conventional enteric seamless capsules, it is possible to encapsulate drugs and active ingredients that react with metal ions, and the range of choice of contents is expanded. There is expected.

Furthermore, the inclusion of polyphenol in the capsule film is expected to have antibacterial and antioxidative effects, and the capsule can be stored for a longer period of time.
From the above, the seamless capsule of the present embodiment is useful in a wide range of fields such as food, cosmetics, and pharmaceuticals, and more particularly useful in the food and pharmaceutical fields.

Claims (6)

A seamless capsule with a capsule coating,
The capsule film is a seamless capsule containing a galactose partial degradation product of galactoxyloglucan and polyphenol.   The seamless capsule according to claim 1, wherein the galactose partial degradation product is obtained by decomposing 30 to 55% of galactose.   The seamless capsule according to claim 1 or 2, wherein the polyphenol is a tea extract. The seamless capsule according to claim 1, wherein the capsule film has acid resistance. Capsules containing the galactose partial decomposed product and the polyphenol by dropping a first aqueous solution containing a galactose partial decomposed product of galactoxyloglucan and water into a second aqueous solution containing polyphenol and water. Comprising a step of forming a seamless capsule having a film ;
The concentration of the partial decomposition product of galactose in the first aqueous solution is 0.5 to 3.0% by mass,
A method for producing a seamless capsule , wherein the concentration of the polyphenol in the second aqueous solution is 0.25 to 10% by mass . The method for producing a seamless capsule according to claim 5 , wherein a tea extract is used as the polyphenol.

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