JP6268834B2 - Label for cell culture containers - Google Patents

Description

  The present invention relates to a label for attaching to a cell culture container, a cell culture container and a label kit, and a cell culture container to which a label is attached.

  The in vitro fertilized egg (zygote) is produced by fertilizing sperm and ovum in a culture system, and the fertilized egg goes through the cleavage, morula, and blastocyst stages, and then emerges from the zona pellucida It is possible to culture up to the blastocyst stage. Assistive reproductive technology (ART) to transfer a fertilized egg from the cleavage to the blastocyst stage to the uterus to give birth to a baby is not limited to the livestock region. Established in infertility medicine.

  In such fields as infertility medical care and reproductive assistance medical care, there is a problem of mix-up of specimens such as sperm, ovum, and fertilized egg. In particular, in this type of treatment, the number of times that the specimen is transferred from container to container is large. Also in fields such as regenerative medicine, it is very important to prevent specimens from being mixed because tissues and cells collected from patients are cultured and transplanted again into the body of the person.

  In order to prevent such a mix-up, a patient name or ID is usually written on the container itself, and a label describing the patient name is attached. In order to automate reading, a method using a bar code or an IC chip has been proposed.

  For example, as described in Patent Document 1, if a label is affixed to the side of a container, there is a high risk of spilling contents when reading the information described on the label. It was necessary to affix on the surface. However, when a label is attached to the container lid, there is an essential problem that it is mistaken for a lid of another container at the time of operation, and it is important to attach a label to the container body. In addition, when the label is attached to the container, information such as the patient name is entered, or when a record is added when the container is taken out of the storage location, the label is attached to the side or back of the container. It was difficult to re-enter in practice.

Japanese Patent No. 4862704

  An object of this invention is to provide the label for cell culture containers which can be affixed on a cell culture container main body in the state which can read information easily, and can be filled in after sticking.

The present inventors have a label comprising a sheet-like base material, an adhesive layer formed on one side of the sheet-like base material, and a release base material that is detachably disposed on the sheet-like base material via the adhesive layer. In
When the release substrate is at least partially peeled from the adhesive layer, the exposed surface of the part of the release substrate that is not peeled from the adhesive layer, the exposed surface of the sheet-like substrate where the adhesive layer is not formed, or the sheet shape Of the surface on which the adhesive layer of the substrate is formed, the exposed surface where the adhesive layer is not formed constitutes the entry area,
When the adhesive layer exposed surface exposed by peeling at least partially from the adhesive layer is attached to the outside of the bottom or side wall of the cell culture container, in a top view from the opening side of the cell culture container, It has been found that the above problem can be solved by configuring the writing area to exist outside the outer periphery of the bottom.

That is, the present invention includes the following inventions.
(1) A label for attaching to the outside of the bottom of the cell culture container or the outside of the side wall of the cell culture container having a bottom, a side wall, and an opening,
A sheet-like base material, an adhesive layer formed on one side of the sheet-like base material, and a release base material that is detachably disposed on the sheet-like base material via the adhesive layer,
When the release substrate is at least partially peeled from the adhesive layer, the exposed surface of the release substrate that is not peeled from the adhesive layer, the side of the sheet-like substrate on which the adhesive layer is not formed Of the exposed surface, or the exposed surface of the sheet-like substrate on which the adhesive layer is formed, the exposed surface on which the adhesive layer is not formed constitutes an entry area,
When the adhesive layer exposed surface that is exposed by at least partially peeling the release substrate from the adhesive layer is affixed to the outside of the bottom of the cell culture vessel or the outside of the side wall, from the opening side of the cell culture vessel. In the top view, the filling area is configured to exist outside the bottom outer periphery,
label.
(2) A label for applying to the outside of the bottom of the cell culture container,
A score line is formed in the release substrate, and the release substrate is configured to be partially removable from the adhesive layer.
The exposed surface of the part that is not peeled off from the adhesive layer of the peeling substrate constitutes the entry area,
The label according to (1).
(3) A label for attaching to the outside of the bottom of a cell culture container having a circular bottom,
The cut line of the peeling substrate is formed in an arc shape along the outer periphery of the circular bottom of the cell culture container when the label is attached to the outside of the bottom of the cell culture container.
The label according to (2).
(4) A label for attaching to the outside of the bottom of a cell culture container having a culture region that can be observed through the center of the bottom,
When the label is attached to the outside of the bottom of the cell culture container, it has a cut portion in an area corresponding to the culture area,
The label according to (2) or (3).
(5) The label according to any one of (1) to (4), wherein the sheet-like substrate and the adhesive layer are transparent.
(6) A cell culture container having a bottom, a side wall, and an opening, a sheet-like base material, an adhesive layer formed on one side of the sheet-like base material, and peeling to the sheet-like base material via the adhesive layer A label comprising a release substrate which is arranged in a possible manner,
When the release substrate is at least partially peeled from the adhesive layer, the exposed surface of the release substrate that is not peeled from the adhesive layer, the side of the sheet-like substrate on which the adhesive layer is not formed Of the exposed surface, or the exposed surface of the sheet-like substrate on which the adhesive layer is formed, the exposed surface on which the adhesive layer is not formed constitutes an entry area,
When the adhesive layer exposed surface that is exposed by at least partially peeling the release substrate from the adhesive layer is affixed to the outside of the bottom of the cell culture vessel or the outside of the side wall, from the opening side of the cell culture vessel. In a top view, a label configured such that the entry area exists outside the outer periphery of the bottom,
Including kit.
(7) The kit according to (6), wherein the cell culture container has a culture region that can be permeated and observed at the center of the bottom.
(8) A cell culture container having a bottom, a side wall, and an opening, the label being affixed to the outside of the bottom or the outside of the side wall,
The label includes a sheet-like base material, an adhesive layer formed on one side of the sheet-like base material, and a release base material that is detachably disposed on the sheet-like base material via the adhesive layer,
An adhesive layer exposed surface that is exposed by at least partially peeling the release substrate from the adhesive layer is affixed to the outside of the bottom or side wall of the cell culture container;
An exposed surface of a portion of the release substrate that is not peeled from the adhesive layer, an exposed surface of the sheet-like substrate on which the adhesive layer is not formed, or the adhesive layer of the sheet-like substrate is formed. The exposed surface of the surface on which the adhesive layer is not formed constitutes an entry area,
In the top view from the opening side of the cell culture container, the label entry area exists outside the outer periphery of the bottom,
The cell culture container.
(9) The cell culture container according to (8), wherein the cell culture container has a culture region in which transmission observation is possible at the center of the bottom.

  According to the present invention, a label for a cell culture container that can be attached to a cell culture container body in an easy-to-read state and can be filled in after the application is provided. The problem of sample mix-up can be avoided.

It is the schematic which shows the upper surface view of one Embodiment of the label of this invention. It is the schematic which shows the vertical sectional view of one Embodiment of the label of this invention. In one Embodiment of the label of this invention, it is the schematic which shows the upper surface view from the opposite side to a sheet-like base material when the peeling base material of a label is partially peeled from the adhesion layer. In one Embodiment of the label of this invention, it is the schematic which shows a vertical sectional view when the peeling base material of a label is partially peeled from the adhesion layer. In one Embodiment of the label of this invention, it is the schematic which shows the top view from the container opening part state of the state which affixed the label on the bottom outer side of the cell culture container. In one Embodiment of the label of this invention, it is the schematic which shows the vertical sectional view of the state which affixed the label on the bottom outer side of the cell culture container. In one Embodiment of the label of this invention, it is the schematic which shows the vertical sectional view of the state which affixed the label on the outer side wall of the cell culture container. It is the schematic which shows the upper surface view of one Embodiment of the label of this invention. It is the schematic which shows the vertical sectional view of one Embodiment of the label of this invention. In one Embodiment of the label of this invention, it is the schematic which shows the upper surface view from the opposite side to a sheet-like base material when the peeling base material of a label peels from an adhesion layer. In one Embodiment of the label of this invention, it is the schematic which shows a vertical sectional view when the peeling base material of a label peels from an adhesion layer. In one Embodiment of the label of this invention, it is the schematic which shows the top view from the container opening part state of the state which affixed the label on the bottom outer side of the cell culture container. In one Embodiment of the label of this invention, it is the schematic which shows the vertical sectional view of the state which affixed the label on the bottom outer side of the cell culture container. In one Embodiment of the label of this invention, it is the schematic which shows the vertical sectional view of the state which affixed the label on the outer side wall of the cell culture container. It is the schematic which shows the upper surface view of one Embodiment of the label of this invention. In one Embodiment of the label of this invention, it is the schematic which shows the top view from the container opening part state of the state which affixed the label on the bottom outer side of the cell culture container. In one Embodiment of the label of this invention, it is the schematic which shows the vertical sectional view of the state which affixed the label on the bottom outer side of the cell culture container. It is the schematic which shows the upper surface view of one Embodiment of the label of this invention. In one Embodiment of the label of this invention, it is the schematic which shows the top view from the container opening part state of the state which affixed the label on the bottom outer side of the cell culture container. In one Embodiment of the label of this invention, it is the schematic which shows the vertical sectional view of the state which affixed the label on the bottom outer side of the cell culture container.

  The present invention will be described below.

In one embodiment, the label is for applying to the bottom outer side or side wall outer side of a cell culture container having a bottom, a side wall, and an opening,
A sheet-like base material, an adhesive layer formed on one side of the sheet-like base material, and a release base material that is detachably disposed on the sheet-like base material via the adhesive layer,
When the release substrate is at least partially peeled from the adhesive layer, the exposed surface of the part of the release substrate that is not peeled from the adhesive layer, the exposed surface of the sheet-like substrate where the adhesive layer is not formed, or the sheet shape Of the surface on which the adhesive layer of the substrate is formed, the exposed surface where the adhesive layer is not formed constitutes the entry area,
When the adhesive layer exposed surface exposed by peeling at least partially from the adhesive layer is attached to the outside of the bottom or side wall of the cell culture container, in a top view from the opening side of the cell culture container, The writing area is configured to exist outside the outer periphery of the bottom.

  In one embodiment, the label can be affixed to a cell culture container having a bottom, a side wall, and an opening commonly used in the art. For example, it can be attached to a general-purpose petri dish capable of culturing cells at the bottom. As a general-purpose petri dish, an opening part and a bottom part are preferably circular, and an opening width is preferably 30 to 60 mm, particularly 35 mm. In one embodiment, the label is configured to be applied to the outside of the bottom or side wall of the cell culture container. The bottom outer side refers to the opposite side to the inner side of the bottom container where cells are placed and cultured in the cell culture container, in other words, the back side of the container. The side wall outside refers to the opposite side to the inside of the container housing part that contains cells and culture solution in the cell culture container, in other words, the outside of the container housing part. Hereinafter, the cell culture container may be simply referred to as a container.

  In one embodiment, the label is suitably used for sticking to the outside of the bottom of a cell culture container, preferably having a round bottom. In one embodiment, the label is also preferably used to be applied to the outside of the bottom of a cell culture container having a culture region that can be observed through the center of the bottom. The culture region is a region where cells are arranged and cultured at the bottom of the cell culture container. The center portion does not need to be an exact center at the bottom portion, and may not be an end of the bottom portion. The culture region is often arranged at the center of a conventional cell culture vessel from the viewpoint of operability during culture and observation. If the label is transparent, there is no problem, but if the label is not transparent, it is preferable that the label and the culture region are attached so as not to overlap when the label is attached to the outside of the bottom. Therefore, it is preferable that when the label is applied to the outside of the bottom portion, the label is distinguished from the other region of the bottom portion so that it can be easily understood. For example, the culture region may be distinguished by surrounding with an inner wall, or may be distinguished by simply surrounding with a line.

  The culture region is preferably a region that is located in the center of the bottom of the cell culture container and that allows permeation observation. The culture region where transmission observation is possible is preferably transparent, preferably made of a transparent material, and is preferable because it is easy to observe when transmitting light through the culture region and observing it under a microscope. Translucent includes translucent, and the transparent material includes translucent material. The label is preferably configured and / or affixed so as not to impair the transmission observation of the culture region. Parts other than the culture region at the bottom of the cell culture container may be transparent, or the entire cell culture container may be formed of a transparent material. Since cell culture containers such as petri dishes commonly used in this technical field are often transparent as a whole, in one embodiment, the label can be suitably used for conventional petri dishes.

  In one embodiment, the label includes a sheet-like substrate, an adhesive layer formed on one side of the sheet-like substrate, and a release substrate that is detachably disposed on the sheet-like substrate via the adhesive layer. Although the shape of the label is not particularly limited, an elongated shape is preferable because, for example, an entry region exists outside the outer periphery of the bottom when attached to a cell culture container, and for example, a strip shape. Specific examples include an elongated circle (such as an ellipse) and a polygon (such as a rectangle), preferably a rectangle. The length of the label in the longitudinal direction (long side length in the case of a rectangle) is preferably 1 to 10 cm, and the width of the label (short side length in the case of a rectangle) is preferably 0.5 to 5 cm. is there. The label is preferably affixed so that the longitudinal direction protrudes outside the outer periphery of the bottom. If it is such a size, sufficient space can be secured for entry, and it does not get in the way during installation or work.

  When the release substrate is at least partially peeled from the adhesive layer, the exposed surface of the part of the release substrate that is not peeled from the adhesive layer, the exposed surface of the sheet-like substrate where the adhesive layer is not formed, or the sheet shape Of the surface on which the adhesive layer of the substrate is formed, the exposed surface where the adhesive layer is not formed constitutes the writing area. In an embodiment in which the release substrate is partially peeled from the adhesive layer, the release substrate is preferably formed with a score line (for example, a half cut). By forming the score line, partial peeling of the peeling substrate can be easily performed. The cut line may be perforated.

  When the adhesive layer exposed surface that is exposed by at least partially peeling the release substrate is attached to the bottom outer side or the side wall outer side of the cell culture container, in the top view from the opening side of the cell culture container, from the outer periphery of the bottom It is configured so that an entry area exists outside. The presence of the writing area outside the outer periphery of the bottom means that the writing area protrudes outward from the outer periphery of the bottom when the adhesive layer exposed surface of the label is attached to the cell culture container. Since the label can be pasted so that the entry area protrudes outward from the outer periphery of the bottom, information can be additionally written after the label is pasted. In addition, unlike the case of appending to a label simply affixed to the side wall of the container (that is, when appending to the side wall of the container), the cell culture container can be added without being lifted. The risk of spilling and the risk that cells such as fertilized eggs move at the bottom of the container and individual cells cannot be identified can be avoided.

  It is preferable that the writing area exists in one direction outside the outer periphery of the bottom. The outer one direction means that the protrusion outward from the outer periphery of the bottom is only one direction and does not protrude in two or more directions. For example, when a strip-shaped label is affixed to a cell culture container, it can be easily held without interfering with the label by holding it from the opposite side to the protruding direction. Also, by gathering information in one place, there is an effect that it is easy to see.

  The entry area is an area for entering necessary information, for example, information on a specimen cultured in a cell culture container to which a label is attached. The information may be completed or entered before the user applies the label. When the adhesive layer exposed surface exposed by peeling the release substrate is applied to the outside of the bottom of the cell culture container, the exposed surface of the part that does not peel from the adhesive layer of the release substrate or the adhesive layer of the sheet-like substrate is formed The exposed surface where the adhesive layer is not formed becomes the entry area. In that case, the label is affixed so that the outer side of the bottom of the container and the exposed surface of the adhesive layer are in contact with each other without being folded. On the other hand, when the adhesive layer exposed surface exposed by peeling the release substrate is affixed to the outside of the side wall of the cell culture container, the exposed surface on the side where the adhesive layer of the sheet-like substrate is not formed becomes the entry area. . In that case, the label is folded in the vicinity of the boundary between the exposed surface of the adhesive layer and the other part so that the exposed surface of the adhesive layer is on the outside (preferably at a right angle, valley-folded when viewed from the sheet-like substrate side) The layer exposed surface is affixed to the outside of the side wall of the container, and the portions other than the affixed portion of the label are arranged on substantially the same plane as the outside of the container bottom. Although it is not necessary to bend it strictly at the boundary between the adhesive layer exposed surface and other portions, it is preferable from the viewpoint of operability that the adhesive layer exposed surface is all attached to the outside of the side wall of the container. This is because if the exposed surface of the adhesive layer is left, it may adhere to a laboratory table or the like, or contaminants may adhere. In any of these embodiments, when the cell culture container with the label attached is placed on a surface such as a table, the label entry area is also exposed on the surface of the table, that is, the upper surface from the container opening side. Since it is arranged in an exposed state, it can be entered in the entry area with the label attached. That is, since the cell culture container and the label are grounded on the same surface, it is possible to easily fill in the entry area.

  Examples of information entered in the entry area include a patient name, a patient identification number (such as a medical record number and a culture control number), a specimen name, a date, and a process name. Also, an identification symbol such as a bar code, QR code, or Anoto dot may be entered / printed. These pieces of information may be entered in advance in the entry area depending on the contents, may be entered by the user, or may be added to the information entered in advance. In addition, when the label is attached to the container and the filling area is transparent (for example, when the sheet-like base material or the adhesive layer is transparent), the adhesive layer of the sheet-like base material is attached to the outside of the bottom of the container. When sticking outside the side wall of the container to the exposed surface on the side where the film is not formed, the exposed surface of the part not peeled off from the adhesive layer of the release substrate or the exposed surface on the side where the adhesive layer of the sheet-like substrate is not formed In addition, it may be printed with reversed characters or the like so that the information can be visually observed from the cell culture container side. In that case, it is preferable that the reversed characters are printed in advance because it is difficult for the user to enter them.

  The adhesive layer exposed surface is a region where the adhesive layer is exposed by peeling the release substrate from the adhesive layer, and the label can be applied to the outside of the bottom or side wall of the cell culture container via the adhesive layer exposed surface. it can. In one embodiment, the release substrate is preferably formed with a score line so as to be partially peelable from the adhesive layer, and the release substrate is partially peeled along the score line to thereby provide an adhesive layer. Is partially exposed to form an adhesive layer exposed surface. In that case, preferably, the pressure-sensitive adhesive layer is formed on the entire surface of one side of the sheet-like base material, and the peeling base material is disposed on the whole surface of the pressure-sensitive adhesive layer before peeling of the peeling base material, and a part thereof is cut. It peels along the line. In another embodiment, the pressure-sensitive adhesive layer is partially formed on one side of the sheet-like substrate, and the release substrate disposed on the entire surface of the partially formed pressure-sensitive adhesive layer is peeled off to be bonded. The area where the layer is exposed is the adhesive layer exposed surface.

From the viewpoint of having a writing area outside the outer periphery of the bottom in the top view from the opening side of the cell culture container when the label is attached to the bottom or side wall of the cell culture container via the adhesive layer exposed surface, The exposed surface is preferably formed at the end of the label. A configuration in which the adhesive layer exposed surface is present in a strip shape along one short side of the rectangular label is preferable. The larger the area of the adhesive layer exposed surface, the more stable the label can be affixed to the container, thereby reducing the risk that the label during work will be peeled off. On the other hand, the smaller the area of the exposed surface of the adhesive layer, the larger the area of the entry area, which is the remaining part, so that more information can be entered. Therefore, it is preferable to adjust the area of the adhesive layer exposed surface while taking into account the amount of information to be entered. For example, the area of the adhesive layer exposed surface with respect to the area of the label is preferably 10 to 80%, more preferably 20 to 50%. Moreover, from the viewpoint of sticking the label stably, the area of the adhesive layer exposed surface is preferably 0.4 to 9 cm ² .

  In one embodiment, the sheet-like substrate and the adhesive layer are transparent, preferably made of a transparent material, and the adhesive layer exposed surface is preferably transparent. The term “transparent” is sufficient if it is transparent to the extent that transmission can be observed, and translucency is included. Transparent materials also include translucent materials. If the adhesive layer exposed surface is transparent, when the label is applied to the bottom of the cell culture container, observation of the specimen in the culture region is not hindered even if the culture region and the adhesive layer exposed surface overlap. Therefore, the adhesive layer exposed surface, that is, the adhesive surface can be widened, and the label can be stably applied. For example, when the label is attached to the bottom of the cell culture container, almost the entire bottom of the cell culture container (for example, 70% or more, 80% or more, or 90% or more) is in contact with the adhesive layer exposed surface. It can be configured. When the outer periphery of the bottom of the cell culture container is circular, the adhesive layer exposed surface of the label is also preferably circular.

  The peeling substrate is formed with a score line, the peeling substrate is partially peeled along the score line, and the adhesive layer exposed surface is attached to the outside of the bottom of the cell culture container, thereby allowing the adhesion layer of the peeling substrate. In the embodiment in which the exposed surface of the part that is not peeled off is used as the entry area, the cut line of the peeling base material follows the outer periphery of the bottom shape of the cell culture container when the label is applied to the outside of the bottom of the cell culture container. Preferably it is formed. When sticking to the outside of the bottom of a cell culture container with a circular bottom, when the cut line of the peeling substrate is attached to the outside of the bottom of the cell culture container, along the circular outer periphery of the cell culture container, For example, it is preferably formed in an arc shape such as a semicircular shape. Thereby, at the time of label sticking, the area which an adhesion layer and a container bottom part contact can be taken wide, and more stable sticking becomes possible. When the adhesive layer is partially formed on one side of the sheet-like substrate, the boundary between the exposed surface of the adhesive layer and the entry area is determined when the label is applied to the outside of the bottom of the cell culture container. For example, it is preferably formed in a circular arc shape such as a semicircular shape along a circular outer periphery.

  In addition, the label for applying to the outside of the bottom of the cell culture container having a culture region that can be observed through the center of the bottom is an area corresponding to the culture region when the label is attached to the outside of the bottom of the cell culture container, That is, it is preferable to have a cut portion that does not have a label in a region overlapping the culture region. In other words, having a cut portion means that the label has a shape having a dent or chip. Since the label is affixed to the bottom outer side through the adhesive layer exposed surface, and the cut portion is present in the region corresponding to the culture region, the cut portion is preferably present on the adhesive layer exposed surface. For example, in a mode in which a rectangular label is attached to a cell culture container having a circular culture region at the bottom center, a shape corresponding to the circular culture region is provided so that the circular culture region and the rectangular label do not overlap at the time of application. It is preferable that a cut portion is formed. In that case, the shape of the cut portion may be a circle corresponding to the entire culture region, or a shape lacking a circle partially corresponding to the culture region. Due to the presence of the cut portion, even when the label is attached to the bottom of the container, there is no overlap between the culture region and the label, so that observation of the specimen in the culture region is not hindered.

  In one embodiment, the label is formed by forming a pressure-sensitive adhesive layer on one side of a sheet-like base material using a method usually used in the art, and disposing the release base material so as to be peelable from the sheet-like base material via the pressure-sensitive adhesive layer Can be manufactured. In one embodiment, the adhesive layer is formed on the entire surface of one side of the sheet-like substrate, and the release substrate is also disposed on the entire surface of the adhesive layer before peeling. In another embodiment, the pressure-sensitive adhesive layer is formed on a part of one side, preferably the end, of the sheet-like substrate, and the release substrate is disposed on the entire surface of the pressure-sensitive adhesive layer before peeling.

  The sheet-like substrate is not particularly limited, and for example, paper and plastic film can be used. As the paper, high-quality paper, coated paper, craft paper, art paper, other various paperboards, processed paper, synthetic paper, and the like can be used. The sheet-like substrate is preferably a material having water resistance. By making it water resistant, it can be used even in a high humidity environment, and damage can be avoided even if it comes into contact with culture medium or water during work. In the embodiment in which the sheet-like base material constitutes the entry area, at least the area constituting the entry area of the sheet-like base material is preferably formed of a fillable material. Only the surface of the area constituting the writing area may be formed of a writable material. Specific examples of materials that can be entered include laser peach paper manufactured by Nisshinbo Postal Chemical Co., Ltd. Examples of the water-resistant and fillable material include YUPO (registered trademark) paper manufactured by YUPO Corporation.

  Although it does not restrict | limit especially as an adhesive used for an adhesion layer, For example, a rubber-type adhesive which has synthetic rubbers as a main component, such as natural rubber, a butadiene-styrene rubber, an acrylic rubber, acrylic acid, butyl acrylate, etc. as a main component Commonly used pressure-sensitive adhesives such as acrylic pressure-sensitive adhesives, silicon-based pressure-sensitive adhesives, and fluorine-based pressure-sensitive adhesives are used. Of these, rubber adhesives and acrylic adhesives are generally used. The pressure-sensitive adhesive layer may be formed of a weakly sticky pressure-sensitive adhesive that can be reapplied. By forming the adhesive layer with a weak adhesive, the label can be pasted on the container once, and then the label can be peeled off from the container, filled in, and pasted again. As a result, when there is a pasting error, the container can be pasted without throwing it away, and when there is an entry mistake, the corrected contents can be pasted without transferring the contents to another container. There are advantages such as being able to fix.

  As a method for applying the pressure-sensitive adhesive, for example, a roll coater, a reverse roll coater, a die coater, a comma coater, or the like can be used. Depending on the viscosity of the adhesive, a gravure coater, a blade coater or the like can be used.

  The release substrate is not particularly limited, and for example, a plastic film can be used. Also, glassine paper, kraft paper or the like formed with a silicon film as a release layer can be suitably used. Moreover, what has a resin laminate layer between paper and a peeling layer can also be used. A plastic film formed with a silicon film as a release layer can also be used. The release substrate is preferably a material having water resistance. By making it water resistant, it can be used even in a high humidity environment, and damage can be avoided even if it comes into contact with culture medium or water during work. As a method of forming the cutting line in the peeling substrate, it can be formed by pressing using a punching blade having a desired shape, a Thomson punching die in which a half-cutting blade is embedded in a wooden mold, or a die.

  The present invention also provides a cell culture container having a bottom, a side wall, and an opening, a kit including the label, and a cell culture container having a bottom, a side wall, and an opening, and the label is attached to the outside of the bottom or the outside of the side wall. In addition, the present invention relates to a cell culture container in which a label writing region exists outside the outer periphery of the bottom part in a top view from the opening side of the cell culture container.

  The shape and the like of the cell culture container are as described above, and the material is not particularly limited. Specifically, inorganic materials such as metal, glass and silicon, plastics (for example, polystyrene resin, polyethylene resin, polypropylene resin, ABS resin, nylon, acrylic resin, fluorine resin, polycarbonate resin, polyurethane resin, methylpentene resin, And organic materials represented by phenol resin, melamine resin, epoxy resin, and vinyl chloride resin). The cell culture vessel can be produced by a method known to those skilled in the art. For example, when a culture container made of a plastic material is manufactured, it can be manufactured by a conventional molding method such as injection molding.

  The cell culture vessel may be subjected to surface hydrophilization treatment such as plasma treatment on the inside from the viewpoint of preventing non-specific adhesion of cultured cells and preventing the drop of culture medium from being biased by surface tension. preferable. More preferably, sterilization such as γ-ray sterilization is performed. The cell culture container with the label attached is preferably sterilized in the same manner. The number of bacteria (bioburden number) adhering to the container before sterilization is preferably 100 cfu / container or less. The label is also preferably sterilized, and more preferably sterilized while wrapped in a packaging bag. The label before sterilization preferably has a number of attached bacteria (number of bioburdens) of 1000 cfu / label or less, and more preferably 100 cfu / label or less. When the number of bacteria before sterilization is small, sterilization is possible without increasing the irradiation dose so much, and there is an advantage that restrictions on the label material are less likely to occur. For example, a material with low gamma ray resistance can be used. In addition, if the number of bacteria on the label side is large, it is necessary to irradiate more than the minimum gamma ray irradiation amount for sterilizing the container alone, and there is a restriction in terms of the gamma ray resistance of the container material. Since the number of bacteria on the label side is equal to or less than that of the container, there is no restriction on the irradiation dose even if the container and the label are put in the same packaging bag.

  The cell culture container may be surface-treated or surface-coated so as to promote the development of a fertilized egg. In particular, in order to promote the development of a fertilized egg, when co-culturing with cells of other organs (for example, endometrial cells or fallopian tube epithelial cells), it is necessary to adhere these cells to a culture vessel in advance. . In such a case, it is advantageous to coat a cell adhesive material on the inner surface of the culture vessel.

  The cells to be cultured are not particularly limited, and examples include fertilized eggs, egg cells, ES cells (embryonic stem cells), and iPS cells (artificial pluripotent stem cells). An egg cell refers to an unfertilized egg cell, and includes an immature oocyte and a mature oocyte. After fertilization, the fertilized egg increases in number of cells from the 2 cell stage, the 4 cell stage, and the 8 cell stage by cleavage, and develops into a blastocyst through a morula. Fertilized eggs include early embryos such as 2-cell embryos, 4-cell embryos and 8-cell embryos, morulas, blastocysts (including early blastocysts, expanded blastocysts and escaped blastocysts). A blastocyst means an embryo composed of external cells with the potential to form the placenta and internal cell masses with the potential to form embryos. ES cells refer to undifferentiated pluripotent or totipotent cells obtained from the inner cell mass of a blastocyst. An iPS cell refers to a cell having a pluripotency similar to that of an ES cell by introducing several types of genes (transcription factors) into somatic cells (mainly fibroblasts). That is, the cell includes an aggregate of a plurality of cells such as a fertilized egg and a blastocyst.

  The cell culture vessel is preferably suitable for culturing mammalian and avian cells, particularly mammalian cells. Mammals refer to warm-blooded vertebrates, for example, primates such as humans and monkeys, rodents such as mice, rats and rabbits, pets such as dogs and cats, and domestic animals such as cattle, horses and pigs. Is mentioned.

  Hereinafter, preferred embodiments of the present invention will be described with reference to the drawings, but the present invention is not limited to the following embodiments.

  One embodiment of the label of the present invention is shown in FIGS. FIG. 1 is a top view of the label as viewed from the peeling substrate side. A vertical sectional view thereof is shown in FIG. In this embodiment, FIG. 3 shows a top view from the side opposite to the sheet-like substrate when the release substrate of the label is partially peeled from the adhesive layer, and FIG. 4 shows a vertical sectional view thereof. Moreover, the top view from the container opening part state of the state which affixed the label on the bottom outer side of the cell culture container is shown in FIG. 5, and the vertical sectional view is shown in FIG.

  As shown in FIGS. 1 and 2, the label 1 has a rectangular strip shape, and is a sheet-like base material 2, an adhesive layer 3 formed on one side of the sheet-like base material 2, and a sheet via the adhesive layer 3. The base material 2 and the peeling base material 4 (4a and 4b) adhere | attached so that peeling is possible. The peeling substrate 4 is formed with a cut line (half cut) 7 so that the peeling substrate can be partially peeled from the adhesive layer. The pressure-sensitive adhesive layer is formed on the entire surface of one side of the sheet-like substrate, and the release substrate is disposed on the entire surface of the pressure-sensitive adhesive layer before the release substrate is peeled off. As shown in FIGS. 3 and 4, when a part (4a) of the peeling substrate 4 is peeled from the adhesive layer 3, the exposed surface of the part (4b) that is not peeled off from the adhesive layer of the peeling substrate is an entry region. 5 is configured. The pressure-sensitive adhesive layer exposed surface 6 is formed at the end of the label, and exists in a strip shape along one short side of the rectangular label. 5 and 6, when the adhesive layer exposed surface 6 exposed by peeling a part (4a) of the peeling substrate 4 is attached to the bottom outer side 9 ′ of the cell culture container 8, the cell In the top view from the opening 11 side of the culture container, the writing region 5 is configured to exist in one direction outside the outer periphery of the bottom. The label is affixed so that the bottom outer side 9 'of the container and the adhesive layer exposed surface 6 are in contact with each other without being bent.

  The cell culture vessel 8 has a bottom portion 9, a side wall 10, and an opening portion 11, the outer periphery of the bottom portion 9 is circular, has a circular culture region 12 in the center, and has an inner wall 13 surrounding the culture region 12. The cell culture container is preferably made of a transparent material as a whole including the culture region. In this embodiment, since the label and the culture region do not overlap with the label attached, the sheet-like substrate and the adhesive region of the label may not be transparent, but may be transparent.

  FIG. 7 shows a vertical sectional view when the label is attached to the outside of the side wall 10 of the cell culture container 8. When sticking the adhesive layer exposed surface 6 exposed by partially peeling the release substrate to the outside of the side wall 10 of the cell culture vessel 8, the exposed side of the sheet-like substrate 2 on which the adhesive layer 3 is not formed is exposed. The surface becomes the entry area 5. In that case, the label is folded at a right angle at the boundary between the pressure-sensitive adhesive layer exposed surface 6 and the other part so that the pressure-sensitive adhesive layer exposed surface 6 is outside (valley fold as viewed from the sheet-like substrate side), and the pressure-sensitive adhesive layer is exposed. The surface 6 is affixed to the outside of the side wall 10 of the container, and a portion other than the affixed portion of the label, that is, the writing area 5 is arranged on substantially the same plane as the container bottom outer side 9 ′.

  Another embodiment of the label of the present invention is shown in FIGS. FIG. 8 is a top view of the label as viewed from the peeling substrate side. A vertical sectional view thereof is shown in FIG. In this embodiment, when the peeling base material of the label is peeled from the adhesive layer, a top view from the side opposite to the sheet-like base material is shown in FIG. 10, and a vertical sectional view thereof is shown in FIG. Further, FIG. 12 shows a top view from the container opening side in a state where the label is attached to the outside of the bottom of the cell culture container, and FIG. 13 shows a vertical sectional view thereof.

  Similar to the above embodiment, the label 1 is in the form of a rectangular strip, the sheet-like substrate 2, the adhesive layer 3 formed on one side of the sheet-like substrate 2, and the sheet-like substrate via the adhesive layer 3. The material 2 and the peeling base material 4 adhere | attached so that peeling is possible are provided. In this embodiment, the pressure-sensitive adhesive layer is partially formed on one end of the sheet-like substrate, and the release substrate is disposed on the entire surface of the pressure-sensitive adhesive layer before the release substrate is peeled off. Then, as shown in FIGS. 10 and 11, the portion exposed by peeling the release substrate 4 from the adhesive layer 3 becomes the adhesive layer exposed surface 6. The pressure-sensitive adhesive layer 3 and the pressure-sensitive adhesive layer exposed surface 6 are formed at the end of the label, and exist in a strip shape along one short side of the rectangular label. Moreover, the exposed surface in which the adhesion layer 3 is not formed among the surfaces in which the adhesion layer 3 of the sheet-like base material 2 is formed constitutes the writing area 5. Then, as shown in FIGS. 12 and 13, when the adhesive layer exposed surface 6 exposed by peeling the release substrate 4 is attached to the bottom outside 9 ′ of the cell culture container 8, the opening 11 of the cell culture container is used. In the top view from the side, the writing area 5 is configured to exist in one direction outside the outer periphery of the bottom. The label is affixed so that the bottom outer side 9 'of the container and the adhesive layer exposed surface 6 are in contact with each other without being bent. The cell culture container 8 is the same as that of the said embodiment.

  FIG. 14 shows a vertical sectional view when the label is attached to the outside of the side wall 10 of the cell culture container 8. When the adhesive layer exposed surface 6 exposed by peeling the release substrate is attached to the outside of the side wall 10 of the cell culture vessel 8, the exposed surface of the sheet-like substrate 2 on the side where the adhesive layer 3 is not formed is filled in. Region 5 is entered. In that case, the label is folded at a right angle at the boundary between the pressure-sensitive adhesive layer exposed surface 6 and the other part so that the pressure-sensitive adhesive layer exposed surface 6 is outside (valley fold as viewed from the sheet-like substrate side), and the pressure-sensitive adhesive layer is exposed. The surface 6 is affixed to the outside of the side wall 10 of the container, and a portion other than the affixed portion of the label, that is, the writing area 5 is arranged on substantially the same plane as the container bottom outer side 9 ′. Also in this embodiment, since the label and the culture region do not overlap with the label attached, the sheet-like substrate and the adhesive region of the label may not be transparent, but may be transparent.

  15, 16, and 17, the label sheet-like substrate 2 and the adhesive layer 3 are made of a transparent material, the adhesive layer exposed surface 6 is transparent, and the label 1 is attached to the bottom outside 9 ′ of the cell culture container 8. In this case, an embodiment in which almost the entire bottom portion of the cell culture vessel 8 is in contact with the adhesive layer exposed surface 6 will be described. FIG. 15 is a top view of the label before the peeling substrate 4 (4a and 4b) is peeled from the peeling substrate side. The label 1 has a strip shape in which a rectangle and a circle are connected, and can be peeled from the sheet-like substrate via the sheet-like substrate, the adhesive layer formed on one side of the sheet-like substrate, and the adhesive layer. And a release substrate 4 (4a and 4b) to be bonded. The pressure-sensitive adhesive layer is formed on the entire surface of one side of the sheet-like substrate, and the release substrate is disposed on the entire surface of the pressure-sensitive adhesive layer before the release substrate is peeled off. A cut line (half cut) 7 is formed in the peeling substrate 4. When the part 4a of the peeling substrate 4 is peeled off and the adhesive layer exposed surface 6 is affixed to the outside of the bottom of the cell culture container, the cut line 7 is a semicircle along the circular outer periphery of the cell culture container. It is formed in a shape. FIG. 16 shows a top view from the container opening side in a state where the label is attached to the outside of the bottom of the cell culture container, and FIG. 17 shows a vertical sectional view thereof. In this embodiment, the outer periphery of the bottom of the cell culture vessel 8 is circular, and the part 4a of the label peeling substrate and the adhesive layer exposed surface 6 are also circular. The cell culture container 8 is the same as that of the said embodiment. Although the adhesive layer exposed surface of the label overlaps with the culture region, since the sheet-like base material and the adhesive layer are made of a transparent material, observation of the specimen in the culture region is not hindered. In addition, since almost the entire bottom surface of the cell culture container is in contact with the exposed surface of the adhesive layer, the label can be stably attached.

  FIG. 18, FIG. 19 and FIG. 20 show an embodiment of a label having a cut portion such that the label does not exist in an area corresponding to the culture area when pasted on the outside of the bottom of the cell culture container. In this embodiment, the cell culture container to which the label is attached has a circular bottom portion and a culture region where transmission observation is possible at the center portion. The label 1 includes a sheet-like substrate 2, an adhesive layer 3 formed on one side of the sheet-like substrate 2, and a release substrate 4 (4 a) that is detachably bonded to the sheet-like substrate via the adhesive layer 3. And 4b). The pressure-sensitive adhesive layer 3 is formed on the entire surface of one side of the sheet-like substrate 2, and the release substrate 4 is disposed on the entire surface of the pressure-sensitive adhesive layer 3 before the release substrate 4 is peeled off. A cut line (half cut) 7 is formed in the peeling substrate 4. When the part 4a of the peeling substrate 4 is peeled off and the adhesive layer exposed surface 6 is attached to the outside of the bottom of the cell culture container, the cut line 7 has an arc shape so as to follow the circular outer periphery of the cell culture container. Is formed. FIG. 18 is a top view of the label before the peeling substrate 4 (4a and 4b) is peeled from the peeling substrate side. FIG. 19 shows a top view from the container opening side in a state where the label is attached to the outside of the bottom of the cell culture container, and FIG. 20 shows a vertical sectional view thereof. The cell culture container 8 is the same as that of the said embodiment. The label 1 has a cut portion 14 having a shape in which a circle partially corresponding to the culture region is missing in a rectangular strip shape. In this embodiment, the cut part 14 exists in the part 4a and the adhesion layer exposure surface 6 after the peeling of the peeling base material. The label is affixed so that the culture region and the cut portion overlap each other, and since the sheet-like substrate, the adhesive layer, and the release substrate do not exist in the cut portion, observation of the specimen in the culture region is not hindered. In addition, since the contact area between the bottom of the cell culture container and the exposed surface of the adhesive layer can be increased, stable labeling can be performed.

1: Label 2: Sheet-like substrate 3: Adhesive layer 4: Release substrate 5: Filling area 6: Adhesive layer exposed surface 7: Cut line 8: Cell culture vessel 9: Bottom portion 9 ': Bottom outside 10: Side wall 11: Opening portion 12: culture region 13: inner wall 14: cut portion

Claims (9)

A label for applying to the outside of the bottom or the outside of the side wall of the cell culture container having a bottom, a side wall and an opening,
A sheet-like base material, an adhesive layer formed on one side of the sheet-like base material, and a release base material arranged to be partially peelable on the sheet-like base material via the adhesive layer,
The release substrate when peeling the tacky layer or al section batchwise, the exposed surface of the not peeled from the adhesive layer portion of the release substrate, or the not the adhesive layer of the sheet-like substrate is formed The exposed surface of the side constitutes the entry area,
When affixed adhesive layer exposed surface which is exposed by peeling off the release substrate to the adhesive layer or al section batchwise at the bottom outside or side wall outside of the cell culture vessel, from the opening side of the cell culture vessel In the top view, the configuration is such that the entry area exists outside the outer periphery of the bottom,
label. A label for attaching to the outside of the bottom of the cell culture container,
A score line is formed in the release substrate, and the release substrate is configured to be partially removable from the adhesive layer.
The label of Claim 1 in which the exposed surface of the part which is not peeled from the said adhesion layer of the said peeling base material comprises the said entry area. It is a label for attaching to the outside of the bottom of a cell culture container having a circular bottom,
The cut line of the peeling substrate is formed in an arc shape along the outer periphery of the circular bottom of the cell culture container when the label is attached to the outside of the bottom of the cell culture container.
The label according to claim 2. It is a label for attaching to the outside of the bottom of a cell culture container having a culture region that can be observed through the center of the bottom,
When the label is attached to the outside of the bottom of the cell culture container, it has a cut portion in an area corresponding to the culture area,
The label according to claim 2 or 3.   The label in any one of Claims 1-4 whose said sheet-like base material and the said adhesion layer are transparent. A cell culture container having a bottom, a side wall, and an opening, a sheet-like base material, an adhesive layer formed on one side of the sheet-like base material, and partially peeled to the sheet-like base material via the adhesive layer A label comprising a release substrate which is arranged in a possible manner,
The release substrate when peeling the tacky layer or al section batchwise, the exposed surface of the not peeled from the adhesive layer portion of the release substrate, or the not the adhesive layer of the sheet-like substrate is formed The exposed surface of the side constitutes the entry area,
When affixed adhesive layer exposed surface which is exposed by peeling off the release substrate to the adhesive layer or al section batchwise at the bottom outside or side wall outside of the cell culture vessel, from the opening side of the cell culture vessel In the top view of the label, the label configured so that the entry area exists outside the outer periphery of the bottom,
Including kit.   The kit according to claim 6, wherein the cell culture container has a culture region that allows permeation observation at the center of the bottom. A cell culture container having a bottom, a side wall and an opening, and a label is affixed to the outside of the bottom or the outside of the side wall,
The label is a sheet-like base material, an adhesive layer formed on one side of the sheet-like base material, and a peeling base material that is arranged to be partially peelable on the sheet-like base material via the adhesive layer; With
The adhesive layer exposed surface of the release substrate is exposed by peeling off the sticky layer or al section batchwise are affixed to the bottom outside or side wall outside of the cell culture vessel,
The exposed surface of the part that is not peeled off from the adhesive layer of the release substrate, or the exposed surface on the side where the adhesive layer of the sheet-like substrate is not formed constitutes an entry area,
In the top view from the opening side of the cell culture container, the label entry area exists outside the outer periphery of the bottom,
The cell culture container.   The cell culture container according to claim 8, wherein the cell culture container has a culture region capable of being permeated and observed at the center of the bottom.

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