JP6252058B2 - Method for determining false positive reaction of urinary urobilinogen in qualitative analysis using color reaction and qualitative analyzer using the same determination method - Google Patents
Method for determining false positive reaction of urinary urobilinogen in qualitative analysis using color reaction and qualitative analyzer using the same determination method Download PDFInfo
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本発明は、尿中のウロビリノーゲンと試薬との呈色反応による試薬部の色の変化を光学的に測定する定性分析において尿中ウロビリノーゲンの偽陽性反応を判定する判定方法及び同判定方法を用いた定性分析装置に関する。 The present invention uses a determination method and a determination method for determining a false positive reaction of urinary urobilinogen in a qualitative analysis for optically measuring a change in color of a reagent portion due to a color reaction between urinary urobilinogen and a reagent. The present invention relates to a qualitative analyzer.
従来から、病院等では、尿に含まれる各種成分を分析し、その分析結果から患者の健康状態を判断するために、患者から尿を採取して、これを分析することが行われている。
尿の分析は、大きく分けて定性分析、定量分析及び沈査分析という３種類の分析方法がある。
尿の定性分析は、尿中に特定の成分が存在しているか否かを判断するための分析である。具体的には、定性分析は、被検出物質と呈色反応する試薬部が設けられた試験紙を、試験管の中の検体に浸漬させたり、前記試験紙の試薬部に検体を点着したりする等した後、呈色反応により変化した後の試薬部の色を、反射光度法等の光学的手法により測定する定性分析装置を用いて行われる。
出願人は、前記定性分析装置として、尿を採取したハルンカップから直接、試験紙の試薬部に尿の点着を行い、尿を点着した後の試薬部の呈色反応による色の変化を光学的に測定する定性分析装置を既に提案している（特許文献１）。
出願人が特許文献１で提案した定性分析装置は、複数の試験紙を収容する試験紙保管装置を備え、この試験紙保管装置から試験紙を一枚ずつ取り出し、始めに尿点着位置に移送し、点着後の試験紙を光学測定位置に移送し、測定後の試験紙を廃棄部に移送するように構成されている。この定性分析装置では、光学測定位置において、尿中の特定成分と呈色反応した後の試薬部の色を測定することができるように試験紙を移送するタイミングが決められている。
特許文献１に示す定性分析装置のように、従来の定性分析装置は、検体中の物質と呈色反応した後の試薬部の色を測定するように構成されている。
しかしながら、試薬部の試薬の呈色反応後の色は、必ずしも目的とする被検出物質と一対一の関係にはならず、被検出物質以外の偽反応物質との反応によっても、呈色反応後の色が同一又は類似の色になることがある。
例えば、尿に含まれる偽反応物質による応答は、被検出物質の応答とは、応答速度が異なるが、従来の定性分析装置は、上記したように、検体中の物質と呈色反応した後の試薬部の色を測定するように構成されているため、偽反応物質による応答か、目的とする被検出物質による応答かを判断することはできない。
出願人は、上記した従来の問題点に鑑みて、偽反応物質による応答か、目的とする被検出物質による応答かを判断する定性分析装置を提案した（特許文献２）。
2. Description of the Related Art Conventionally, in hospitals and the like, various components contained in urine are analyzed, and urine is collected from the patient and analyzed in order to determine the health condition of the patient from the analysis result.
There are three types of analysis of urine analysis: qualitative analysis, quantitative analysis and sedimentation analysis.
The qualitative analysis of urine is an analysis for determining whether or not a specific component is present in urine. Specifically, in the qualitative analysis, a test paper provided with a reagent part that performs a color reaction with a substance to be detected is immersed in a specimen in a test tube, or a specimen is spotted on the reagent part of the test paper. Or the like, followed by using a qualitative analyzer that measures the color of the reagent part after the color reaction has changed by an optical technique such as a reflection photometry.
The applicant, as the qualitative analyzer, spotted urine directly on the reagent part of the test paper directly from the Harun cup from which urine was collected, and optically measured the color change due to the color reaction of the reagent part after the urine was spotted. Has already proposed a qualitative analysis device for automatic measurement (Patent Document 1).
The qualitative analysis device proposed by the applicant in Patent Document 1 is provided with a test paper storage device that accommodates a plurality of test papers. The test paper is taken out from the test paper storage device one by one and first transferred to the urine spotting position. The test paper after spotting is transferred to the optical measurement position, and the test paper after measurement is transferred to the disposal unit. In this qualitative analyzer, the timing for transferring the test paper is determined so that the color of the reagent part after color reaction with a specific component in urine can be measured at the optical measurement position.
Like the qualitative analyzer shown in Patent Document 1, the conventional qualitative analyzer is configured to measure the color of the reagent part after color reaction with the substance in the specimen.
However, the color after the color reaction of the reagent in the reagent part does not necessarily have a one-to-one relationship with the target substance to be detected. May be the same or similar colors.
For example, the response due to the pseudo-reactive substance contained in urine is different in response speed from the response of the substance to be detected. However, as described above, the conventional qualitative analyzer has a color reaction with the substance in the specimen as described above. Since it is configured to measure the color of the reagent part, it cannot be determined whether the response is due to a false reaction substance or a target detection target substance.
In view of the above-described conventional problems, the applicant has proposed a qualitative analysis device that determines whether the response is due to a pseudo-reactive substance or the target detected substance (Patent Document 2).
出願人が特許文献２で提案した定性分析装置は、目的とする被検出物質と呈色反応する試薬部を備えた検査体の試薬部の色を、反応前の初期状態を含め検体との接触直後から、呈色反応終了迄の間、所定の時間間隔で複数回検出するように構成されており、これにより、試薬部の呈色反応を経時的に計測することが可能になる。
出願人は、上記した定性分析装置の提案後も、鋭意研究を続け、尿中ウロビリノーゲンの偽陽性反応を判定することができる特性を見出し本発明を発明するに至った。
本発明は、呈色反応を用いた定性分析における尿ウロビリノーゲンの偽陽性反応判定方法及び同判定方法を用いた定性分析装置を提供することを目的としている。
The qualitative analysis apparatus proposed by the applicant in Patent Document 2 is that the color of the reagent part of the specimen including a reagent part that performs a color reaction with the target substance to be detected is brought into contact with the specimen including the initial state before the reaction. It is configured to detect a plurality of times at predetermined time intervals immediately after the completion of the color reaction, thereby making it possible to measure the color reaction of the reagent portion over time.
The applicant has continued intensive research after the proposal of the qualitative analyzer described above, and has found a characteristic capable of determining a false positive reaction of urinary urobilinogen, and has invented the present invention.
An object of the present invention is to provide a false positive reaction determination method for urinary urobilinogen in a qualitative analysis using a color reaction and a qualitative analysis device using the determination method.
上記した目的を達成するために、本発明に係る呈色反応を用いた定性分析における尿ウロビリノーゲンの偽陽性反応判定方法は、尿中ウロビリノーゲンと呈色反応する試薬が設けられた検査体の試薬部に尿を接触させるステップと、呈色反応終了後に試薬部の色を測定するステップと、測定した試薬部の色情報から得られる青成分と緑成分との比率に基づいて尿ウロビリノーゲンの偽陽性反応を判別するステップとから成ることを特徴とする。
前記色情報は、波長６２５ｎｍ〜７４０ｎｍの光を含む赤成分と、波長５００ｎｍ〜５６０ｎｍの光を含む緑成分と、波長４４５ｎｍ〜４８５ｎｍの光を含む青成分とを有し得る。
また、本発明に係る尿ウロビリノーゲンの偽陽性反応判定方法では、尿を接触させていない試薬部の色を測定しておき、該測定結果から得られる色情報を基準として、前記尿を接触させた試薬部の色を測定した色情報から得られる前記赤成分、緑成分及び青成分の色情報を補正してもよい。
さらにまた、本発明に係る尿ウロビリノーゲンの偽陽性反応判定方法では、前記測定した色情報から得られる青成分と緑成分との比率が、所定の値より低ければ偽陽性反応であると判定し得る。
本発明に係る尿定性分析装置は、尿ウロビリノーゲンと呈色反応する試薬部を備えた検査体の試薬部の色を、呈色反応終了後に測定する光学測定手段と、前記光学測定手段により測定された試薬部の色情報から得られる青成分と緑成分との比率に基づいて尿ウロビリノーゲンの陽性反応及び偽陽性反応を判別する判定手段とを備えていることを特徴とする。
In order to achieve the above object, the false positive reaction determination method for urinary urobilinogen in the qualitative analysis using the color reaction according to the present invention is a reagent part of a test body provided with a reagent that performs a color reaction with urinary urobilinogen. Urine contact with urine, step of measuring the color of the reagent part after completion of the color reaction, and false positive reaction of urinary urobilinogen based on the ratio of blue and green components obtained from the color information of the measured reagent part And a step of discriminating between.
The color information may include a red component including light having a wavelength of 625 nm to 740 nm, a green component including light having a wavelength of 500 nm to 560 nm, and a blue component including light having a wavelength of 445 nm to 485 nm.
Further, in the false positive reaction determination method for urine urobilinogen according to the present invention, the color of the reagent part not in contact with urine is measured, and the urine is brought into contact with reference to color information obtained from the measurement result. You may correct | amend the color information of the said red component, green component, and blue component obtained from the color information which measured the color of the reagent part.
Furthermore, in the false positive reaction determination method for urinary urobilinogen according to the present invention, if the ratio of the blue component and the green component obtained from the measured color information is lower than a predetermined value, it can be determined that it is a false positive reaction. .
The urine qualitative analyzer according to the present invention is measured by the optical measuring means for measuring the color of the reagent part of the specimen having a reagent part that is colored with urine urobilinogen after the completion of the color reaction, and the optical measuring means. And determining means for discriminating positive and false positive reactions of urinary urobilinogen based on the ratio of the blue component and the green component obtained from the color information of the reagent part.
本発明に係る呈色反応を用いた定性分析における尿ウロビリノーゲンの偽陽性反応判定方法は、尿中ウロビリノーゲンと呈色反応する試薬が設けられた検査体の試薬部に尿を接触させるステップと、呈色反応終了後に試薬部の色を測定するステップと、測定した試薬部の色情報から得られる青成分と緑成分との比率に基づいて尿ウロビリノーゲンの偽陽性反応を判別するステップとから成るので、呈色反応を用いた定性分析において尿ウロビリノーゲンの偽陽性反応を精度よく判別することが可能になるという効果を奏する。
また、本発明に係る尿定性分析装置は、尿定性分析装置において、尿ウロビリノーゲンと呈色反応する試薬部を備えた検査体の試薬部の色を、呈色反応終了後に測定する光学測定手段と、前記光学測定手段により測定された試薬部の色情報から得られる青成分と緑成分との比率に基づいて尿ウロビリノーゲンの陽性反応及び偽陽性反応を判別する判定手段とを備えているので、呈色反応を用いた定性分析装置において尿ウロビリノーゲンの陽性反応及び偽陽性反応を精度よく判別することが可能になるという効果を奏する。
A false positive reaction determination method for urinary urobilinogen in a qualitative analysis using a color reaction according to the present invention includes a step of bringing urine into contact with a reagent part of a test body provided with a reagent that performs a color reaction with urinary urobilinogen; Since it comprises the steps of measuring the color of the reagent part after completion of the color reaction, and determining the false positive reaction of urinary urobilinogen based on the ratio of the blue component and the green component obtained from the color information of the measured reagent part. In the qualitative analysis using the color reaction, there is an effect that the false positive reaction of urine urobilinogen can be accurately discriminated.
Further, the urine qualitative analyzer according to the present invention is the urine qualitative analyzer, optical measuring means for measuring the color of the reagent part of the test body provided with the reagent part color-reacting with urine urobilinogen after completion of the color reaction. And determining means for discriminating positive and false positive reactions of urinary urobilinogen based on the ratio of the blue component and the green component obtained from the color information of the reagent part measured by the optical measuring means. In the qualitative analyzer using the color reaction, there is an effect that it becomes possible to accurately discriminate between a positive reaction and a false positive reaction of urine urobilinogen.
以下、添付図面に示した一実施例を参照しながら本発明に係る呈色反応を用いた定性分析における尿ウロビリノーゲンの偽陽性反応判定方法の実施の形態について説明していく。 Hereinafter, an embodiment of a false positive reaction determination method for urinary urobilinogen in a qualitative analysis using a color reaction according to the present invention will be described with reference to one embodiment shown in the accompanying drawings.
出願人は、尿試験紙における尿ウロビリノーゲンの呈色反応の経時的な特性を確認するために、尿ウロビリノーゲンを含む尿から成る陽性試料と、試験紙では陽性を示すが確認試験では陰性を示す偽陽性試料とを用意し、各試料を、尿ウロビリノーゲンと呈色反応する試薬が設けられた試験紙の試薬部に点着し、点着直後（本実施例では２秒後）から４秒毎に９回、試薬部の色を測定した。
図１（ａ）及び（ｂ）は、測定結果を赤成分、緑成分及び青成分の三原色に分離してプロットしたグラフであり、各グラフにおいて菱形のプロットは赤成分（Ｒ帯波長光：６２５ｎｍ〜７４０ｎｍ）であり、四角形のプロットは緑成分（Ｇ帯波長光：５００ｎｍ〜５６０ｎｍ）であり、三角形のプロットは青成分（Ｂ帯波長光：４４５ｎｍ〜４８５ｎｍ）である。図１（ａ）は陽性試料の測定結果であり、図１（ｂ）は偽陽性試料の測定結果である。本実施例では、赤成分、緑成分及び青成分は、それぞれの色の反射率である。
図１の測定結果から、陽性試料及び偽陽性試料との間で、赤成分、緑成分及び青成分の経時的変化に顕著な差は見られなかったが、青成分と緑成分との間で逆転傾向が見られた。
図２は、図１に示した測定結果における青成分と緑成分との比率をプロットしたグラフであり、図２（ａ）は陽性試料の測定結果であり、図２（ｂ）は偽陽性試料の測定結果である。
図１及び図２の測定結果から、呈色反応終了後の試薬部の色の青成分と緑成分との比率を用いて尿ウロビリノーゲンの陽性反応と偽陽性反応とを判別することができることを確認した。
In order to confirm the time-dependent characteristics of the urinary urobilinogen color reaction on the urine test paper, the applicant must provide a positive sample consisting of urine containing urinary urobilinogen and a false sample that is positive in the test paper but negative in the confirmation test. A positive sample is prepared, and each sample is spotted on the reagent part of a test paper provided with a reagent that reacts with urine urobilinogen and immediately after spotting (after 2 seconds in this example) every 4 seconds. The color of the reagent part was measured 9 times.
FIGS. 1A and 1B are graphs obtained by separating the measurement results into three primary colors of a red component, a green component, and a blue component, and in each graph, a rhombus plot indicates a red component (R-band wavelength light: 625 nm). The square plot is the green component (G-band wavelength light: 500 nm to 560 nm), and the triangular plot is the blue component (B-band wavelength light: 445 nm to 485 nm). FIG. 1A shows the measurement result of the positive sample, and FIG. 1B shows the measurement result of the false positive sample. In this embodiment, the red component, the green component, and the blue component are the reflectances of the respective colors.
From the measurement results of FIG. 1, there was no significant difference in the temporal change of the red component, the green component, and the blue component between the positive sample and the false positive sample, but between the blue component and the green component. A reversal trend was observed.
2 is a graph in which the ratio of the blue component and the green component in the measurement result shown in FIG. 1 is plotted, FIG. 2 (a) is the measurement result of the positive sample, and FIG. 2 (b) is the false positive sample. It is a measurement result.
From the measurement results of FIG. 1 and FIG. 2, it is confirmed that the positive reaction and false positive reaction of urinary urobilinogen can be discriminated using the ratio of the blue component and the green component of the color of the reagent part after completion of the color reaction. did.
図３は、陽性試料及び偽陽性試料を含む６０個の試料を、それぞれ試験紙の試薬部に点着して呈色反応終了後の試薬部の色を測定し、測定した試薬部の色情報に基づいて青成分と緑成分との比率を算出した結果のグラフである。四角形のプロットが陽性試料であり、菱形のプロットが偽陽性試料である。 FIG. 3 shows 60 pieces of samples including positive samples and false positive samples that are spotted on the reagent part of the test paper, and the color of the reagent part after the completion of the color reaction is measured. It is a graph of the result of having calculated the ratio of a blue component and a green component based on this. Square plots are positive samples and diamond plots are false positive samples.
上記した結果から、尿ウロビリノーゲンと呈色反応する試薬が設けられた検査体の試薬部に尿を接触させ、呈色反応終了後（本実施例では３４秒後）の試薬部の色を測定し、測定した試薬部の色情報から得られる青成分と緑成分との比率に基づいて尿ウロビリノーゲンの陽性反応及び偽陽性反応を判別することが可能になることが確認できる。
具体的には、呈色反応終了後の試薬部の色の青成分と緑成分との比率（青成分／緑成分）が所定の値より低ければ偽陽性反応であると判定することができる。
From the results described above, urine was brought into contact with the reagent part of the specimen provided with a reagent that reacts with urine urobilinogen, and the color of the reagent part after the completion of the color reaction (after 34 seconds in this example) was measured. It can be confirmed that the positive reaction and false positive reaction of urinary urobilinogen can be discriminated based on the ratio of the blue component and the green component obtained from the measured color information of the reagent part.
Specifically, if the ratio (blue component / green component) between the blue component and the green component of the color of the reagent part after the completion of the color reaction is lower than a predetermined value, it can be determined that the reaction is a false positive reaction.
次に、上記した判定方法を実行する尿定性分析装置の実施例について説明していく。
図４は、尿定性分析装置の構成を示す概略ブロック図である。
図中、符号１は検査体を構成する試験紙を示し、この試験紙１には、尿ウロビリノーゲンと呈色反応する試薬が設けられた試薬部１ａが設けられている。
図中符号２は光学測定部であり、該光学測定部２は例えばＣＣＤ等の撮像素子を有し、該撮像素子を用いて試薬部１ａを撮像する。
光学測定部２で撮像された画像は、画像処理部３に送られ、画像処理記憶部３では、撮像された画像から試薬部１ａの赤成分（赤反射率）、緑成分（緑反射率）及び青成分（青反射率）が演算されて記憶される。
判定部４は、画像処理記憶部３に記憶された試薬部の色情報に基づいて、呈色反応終了後の青成分と緑成分との比率を算出し、同比率に基づいて尿ウロビリノーゲンの陽性反応及び偽陽性反応を判別し、プリンタやモニター等からなる出力部５に判定結果を出力する。
Next, an embodiment of a urine qualitative analyzer that executes the above-described determination method will be described.
FIG. 4 is a schematic block diagram showing the configuration of the urine qualitative analyzer.
In the figure, reference numeral 1 denotes a test paper constituting the test body, and this test paper 1 is provided with a reagent part 1a provided with a reagent that reacts with urine urobilinogen.
In the figure, reference numeral 2 denotes an optical measurement unit. The optical measurement unit 2 has an image sensor such as a CCD, and images the reagent unit 1a using the image sensor.
The image captured by the optical measuring unit 2 is sent to the image processing unit 3, and the image processing storage unit 3 uses the captured image to detect the red component (red reflectance) and the green component (green reflectance) of the reagent unit 1 a. And the blue component (blue reflectance) are calculated and stored.
The determination unit 4 calculates the ratio of the blue component and the green component after the completion of the color reaction based on the color information of the reagent unit stored in the image processing storage unit 3, and based on the ratio, the urine urobilinogen is positive The reaction and the false positive reaction are discriminated, and the determination result is output to the output unit 5 including a printer, a monitor and the like.
以下、上記したように構成された尿定性分析装置の作用について簡単に説明していく。
光学測定部２は、尿が点着される前の試薬部１ａの画像を撮像する。同画像は画像処理記憶部３に送られ、画像処理記憶部３は同画像から、尿が点着される前の試薬部１ａの色の赤成分（赤反射率）、緑成分（緑反射率）及び青成分（青反射率）を演算して記憶する。
試薬部１ａに尿が点着されると、光学測定部２は、呈色反応終了後（本実施例では３４秒後）に試薬部１ａを撮像し、その都度、画像処理記憶部３に画像を送る。
画像処理記憶部３は、送られてきた画像から、試薬部１ａの色の赤成分（赤反射率）、緑成分（緑反射率）及び青成分（青反射率）を演算して記憶する。
判定部４は、画像処理記憶部３に記憶された試薬部の色情報に基づいて、呈色反応終了後の青成分と緑成分との比率を算出し、算出した値に基づいて尿ウロビリノーゲンの陽性反応及び偽陽性反応を判別し、プリンタやモニター等からなる出力部５に判定結果を出力する。
具体的には、判定部４は、算出した青成分と緑成分との比率が0.85〜1.0より高い場合には偽陽性反応であると判定して、判定結果を出力部５を介して出力する。
また、陽性と偽陽性が一部混在する領域があるので、グレー領域を0.85〜1.0として、1.0以上を偽陽性、0.85以下を偽陽性と設定することも可能である。
前記判定部４は、必要に応じて、尿が点着される前の試薬部１ａの色の赤成分（赤反射率）、緑成分（緑反射率）及び青成分（青反射率）に基づいて、尿の点着後の画像から得られる色情報を補正して判定をするように構成することができる。
The operation of the urine qualitative analyzer configured as described above will be briefly described below.
The optical measurement unit 2 captures an image of the reagent unit 1a before urine is spotted. The image is sent to the image processing storage unit 3, and the image processing storage unit 3 extracts the red component (red reflectance) and the green component (green reflectance) of the color of the reagent unit 1 a before urine is spotted from the image. ) And the blue component (blue reflectance) are calculated and stored.
When urine is spotted on the reagent part 1a, the optical measuring part 2 images the reagent part 1a after the completion of the color reaction (34 seconds in this embodiment), and in each case, the image processing storage part 3 Send.
The image processing storage unit 3 calculates and stores the red component (red reflectance), green component (green reflectance), and blue component (blue reflectance) of the color of the reagent unit 1a from the sent image.
The determination unit 4 calculates the ratio of the blue component and the green component after completion of the color reaction based on the color information of the reagent unit stored in the image processing storage unit 3, and based on the calculated value, the urinary urobilinogen A positive reaction and a false positive reaction are discriminated, and a determination result is output to the output unit 5 including a printer, a monitor, and the like.
Specifically, the determination unit 4 determines a false positive reaction when the calculated ratio of the blue component and the green component is higher than 0.85 to 1.0, and outputs the determination result via the output unit 5. .
In addition, since there is a region in which some of the positive and false positives are mixed, it is possible to set the gray region as 0.85 to 1.0 and set 1.0 or higher as false positive and 0.85 or lower as false positive.
The determination unit 4 is based on the red component (red reflectance), green component (green reflectance), and blue component (blue reflectance) of the color of the reagent unit 1a before urine is spotted, if necessary. Thus, the determination can be made by correcting the color information obtained from the urine spotted image.
上記した実施例では、点着した後、３４秒後に試薬部の色を測定し、測定結果に基づいて判定を行っているが、測定タイミングは本実施例に限定されることなく、呈色反応が終了した後であれば３４秒後でなくてもよく、たとえば、６０秒後であってもよい。
また、本実施例で設定した0.85〜1.0という値はこれに限定されるものではなく、測定系により変更し得る。
In the above-described embodiment, the color of the reagent portion is measured 34 seconds after spotting, and determination is performed based on the measurement result. However, the measurement timing is not limited to this embodiment, and the color reaction If it is after ending, it may not be 34 seconds later, for example, 60 seconds later.
Further, the value of 0.85 to 1.0 set in the present embodiment is not limited to this and can be changed depending on the measurement system.
１ 試験紙
１ａ 試薬部
２ 光学測定部
３ 画像処理記憶部
４ 判定部
５ 出力部
DESCRIPTION OF SYMBOLS 1 Test paper 1a Reagent part 2 Optical measurement part 3 Image processing memory | storage part 4 Judgment part 5 Output part
Claims (5)
呈色反応終了後に試薬部の色を測定するステップと、
測定した試薬部の赤成分、青成分及び緑成分から成る色情報から得られる青成分と緑成分との比率に基づいて尿ウロビリノーゲンの偽陽性反応を判別するステップと
から成ることを特徴とする
呈色反応を用いた定性分析における尿ウロビリノーゲンの偽陽性反応判定方法。 Contacting the urine with a reagent part of a test body provided with a reagent that reacts with urinary urobilinogen;
Measuring the color of the reagent part after completion of the color reaction;
And determining a false positive reaction of urinary urobilinogen based on the ratio of the blue component and the green component obtained from the measured color information of the red component, the blue component, and the green component. A method for determining a false positive reaction of urinary urobilinogen in a qualitative analysis using a color reaction.
ことを特徴とする請求項１に記載の判定方法。 The color information includes a red component including light having a wavelength of 625 nm to 740 nm, a green component including light having a wavelength of 500 nm to 560 nm, and a blue component including light having a wavelength of 445 nm to 485 nm. The determination method described in 1.
ことを特徴とする請求項１又は２に記載の判定方法。 The red component and the green component obtained from the color information obtained by measuring the color of the reagent part not in contact with urine and measuring the color of the reagent part in contact with urine based on the color information obtained from the measurement result 3. The determination method according to claim 1, wherein the color information of the blue component is corrected.
ことを特徴とする請求項１〜３の何れか一項に記載の判定方法。 The ratio of the blue component and green component obtained from the measured color information is determined to be a false positive reaction if the ratio is lower than a predetermined value. Judgment method.
尿ウロビリノーゲンと呈色反応する試薬部を備えた検査体の試薬部の色を、呈色反応終了後に測定する光学測定手段と、
前記光学測定手段により測定された試薬部の色情報から得られる青成分と緑成分との比率に基づいて尿ウロビリノーゲンの陽性反応及び偽陽性反応を判別する判定手段と
を備えていることを特徴とする尿定性分析装置。 In the urine qualitative analyzer,
An optical measuring means for measuring the color of the reagent part of the test body provided with a reagent part color-reacting with urinary urobilinogen after completion of the color reaction;
And determining means for discriminating a positive reaction and a false positive reaction of urinary urobilinogen based on a ratio between a blue component and a green component obtained from color information of the reagent part measured by the optical measuring means. Urine qualitative analyzer.
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