JP6175519B2 - Condensed tricyclic compounds as Raf kinase inhibitors - Google Patents

Description

  Disclosed herein are fused tricyclic compounds, pharmaceutical compositions comprising at least one such fused tricyclic compound, methods for their preparation, and their use in therapy. Disclosed herein are certain tricyclic compounds that can be useful for inhibiting Raf kinase and for treating disorders mediated by Raf kinase.

  Of interest in cell survival, growth, proliferation and tumorigenesis is the Raf / MEK / ERK pathway (Zebisch et al., Curr Med Chem. 14 (5): 601-623, 2007; Roberts and Der, Oncogene 26 (22): 3291-3310, 2007; Montagut and Settleman, Cancer Lett. 283 (2): 125-134, 2009). Stimulation of the Raf / MEK / ERK signaling pathway can occur after the ligand has bound to the membrane-bound receptor tyrosine kinase. GTP-bound RAS can be activated, which can subsequently promote activation of Raf family proteins (A-Raf, B-Raf, and Raf1, formerly known as C-Raf) (Wellbrock et al., Nat. Rev. Mol. Cell Biol. 5: 875-885, 2004). Mutations of various RAS GTPases and B-Raf kinases in the Raf / MEK / ERK signaling pathway have been reported to constitutively activate the MAPK pathway and increase cell division and survival (Bos, Cancer Res 49: 4682-4689, 1989; Hoshino et al., Oncogene. 18 (3): 813-822, 1999). For example, B-Raf mutations are reportedly found in the majority of human melanoma and thyroid cancer (Davies et al., Nature417: 949-954, 2002) (Cohen et al., J. Nat Cancer Inst. 95 (8): 625-627, 2003; Kimura et al., Cancer Res. 63 (7): 1454-1457, 2003; Pollock and Meltzer, Cancer Cell 2: 5-7, 2002). In addition, a lesser but still significant frequency of B-Raf mutations were found in Barrett's adenocarcinoma (Garnett et al., Cancer Cell 6: 313-319, 2004; Sommerer et al., Oncogene 23 (2) : 554-558, 2004), breast cancer (Davies et al., Nature417: 949-954, 2002), cervical cancer (Moreno-Bueno et al., Clin. Cancer Res. 12 (12): 365-3866, 2006), cholangiocarcinoma (Tannapfel et al., Gut. 52 (5): 706-712, 2003), glioblastoma (Knobbe et al., Acta Neuropathol. (Berl.). 108 (6): 467 -470, 2004), colorectal cancer (Yuen et al., Cancer Res. 62 (22): 6451-6455, 2002; Davies et al., Nature 417: 949-954, 2002), gastric cancer (Lee et al. , Oncogene22 (44): 6942-6945), lung cancer (Brose et al., Cancer Res. 62 (23): 6997-7000, 2002), ovarian cancer (Russell and McCluggage, J. Pathol. 203 (2): 617-619, 2004; Davies et al., Nature 417: 949-954, 2002), pancreatic cancer (Ishimura et al., Cancer Lett. 199 (2): 169-173, 2003), prostate cancer (Cho et al., Int. J. Cancer. 119 (8): 1 858-1862, 2006), and blood cancer (Garnett and Marais, Cancer Cell 6: 313-319, 2004). These reports suggest that B-Raf is one of the most frequently mutated genes in human cancer. B-Raf kinase may be an excellent target for anti-cancer therapy based on preclinical target validation, epidemiology and dragability.

  Inhibitors of Raf kinase are in the inhibition of tumor cell growth and thus cancers such as melanoma, colorectal cancer including colorectal cancer, histiocytic lymphoma, lung adenocarcinoma, small cell lung cancer, pancreas In the treatment of cancer and breast cancer (Crump, Current Pharmaceutical Design 8: 2243-2248, 2002; Sebastien et al., Current Pharmaceutical Design 8: 2249-2253, 2002) and / or cerebral ischemia, stroke after cardiac arrest And its use in the treatment or prevention of disorders related to neurodegeneration resulting from ischemic events, including multiple infarct dementia. Inhibitors of Raf kinase have been found after cerebral ischemic events such as those occurring during head injury, surgery and / or childbirth (York et al., Mol. And Cell. Biol. 20 (21): 8069-8083 , 2000; Chin et al., Neurochem. 90: 595-608, 2004), as well as use in polycystic kidneys (Nagao et al., Kidney Int. 63 (2): 427-437, 2003). I came.

  In addition, certain hyperproliferative disorders may be characterized by excessive activation of Raf kinase function, such as protein mutation or overexpression. Thus, inhibitors of Raf kinase may be useful in the treatment of hyperproliferative disorders such as cancer.

  Small molecule inhibitors of B-Raf kinase are under development for anticancer therapy. Nexavar® (sorafenib tosylate) is a multi-targeted kinase inhibitor, including inhibition of B-Raf kinase, approved for the treatment of patients with advanced renal cell carcinoma and unresectable hepatocellular carcinoma Has been. Other Raf inhibitors such as SB-590885, RAF-265, PLX-4032, GSK2118436 and XL-281 are also disclosed or are in clinical trials.

  Other B-Raf inhibitors are also known. For example, U.S. Patent Application Publication No. 2006/0189627, U.S. Patent Application Publication No. 2006/0281751, U.S. Patent Application Publication No. 2007/0049603, International Publication No. 2007/002325, International Publication No. 2007/002433, See International Publication No. 03/068773 and International Publication No. 2007/013896.

  Certain nitrogen-containing heteroaryl substituted aryl bicyclic compounds have been identified as Raf inhibitors. See, for example, International Publication No. 2007/067444 and US Patent Application Publication No. 2010/0197924.

  Specific Raf kinase inhibitors have also been identified. For example, International Publication No. 2005/062795, International Publication No. 2008/079906, International Publication No. 2008/079909, International Publication No. 2006/066913, International Publication No. 2008/028617 and International Publication No. See 2009/012283.

Disclosed herein are compounds that can inhibit Raf kinases, such as B-Raf kinase. Provided is at least one compound selected from a compound of formula I, stereoisomers thereof, and pharmaceutically acceptable salts thereof:
Where
Q is selected from C and N;
W is selected from C and N;
X is selected from CH ₂ and O;
Y is selected from NR ¹² , O, and S;
Z is selected from O, S, NR ¹³ , CO, SO, SO ₂ , and CR ¹³ R ¹⁴ ;
R ¹ , R ² , R ³ , R ⁴ , R ⁵ , and R ⁶ may be the same or different and are each hydrogen, halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, Alkynyl, -CN, -NR ¹³ R ¹⁴ , -OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ ,- Selected from NR ¹³ CONR ¹⁴ R ¹⁵ , -NR ¹³ CO ₂ R ¹⁴ , -SO ₂ R ¹³ , -NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , and -NR ¹³ SO ₂ R ¹⁴ , where alkyl, alkenyl, alkynyl , Cycloalkyl, heteroaryl, aryl, and heterocyclyl may be substituted with at least one substituent R ¹⁶ , or (R ¹ and R ² ), and / or (R ³ and R ⁴ ), and / or (R ⁵ and R ⁶⁾ is of substitution together with the ring to which they are attached, at least one of the substituents R ¹⁶ A condensed ring selected from even heterocyclyl and heteroaryl rings optionally form; however, when Q is N, R ¹ is absent, and when W is N, R ⁵ is absent;
R ⁷ is selected from hydrogen, halogen, alkyl, —O-alkyl, and —S-alkyl;
R ¹² is selected from hydrogen and alkyl;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''', and NR'SO ₂ R '', where R ', R'', and R''' are H, haloalkyl , Alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R ′ and R ″) and / or (R ″ and R ′ ″) are Together with the atoms to which is attached forms a ring selected from heterocyclyl and heteroaryl rings.

  Comprising at least one pharmaceutically acceptable carrier and at least one compound selected from the compounds of formula (I) described herein, stereoisomers thereof, and pharmaceutically acceptable salts thereof. A pharmaceutical composition is also provided.

  A method of treating a cancer responsive to inhibition of Raf kinase, as described herein, in an amount effective to treat the cancer in a subject in need of such cancer treatment. Also provided is a method comprising administering at least one compound selected from compounds of formula (I), stereoisomers thereof, and pharmaceutically acceptable salts thereof.

  Use of at least one compound selected from a compound of formula (I), a stereoisomer thereof, and a pharmaceutically acceptable salt thereof as described herein in the manufacture of a medicament for inhibiting Raf kinase provide.

  The use of at least one compound selected from a compound of formula (I), a stereoisomer thereof, and a pharmaceutically acceptable salt thereof described herein in the manufacture of a medicament for treating cancer provide.

  The following words, phrases and symbols used herein are generally intended to have the meanings indicated below, except where indicated by the context in which they are used: The following abbreviations and terms have the meanings given throughout.

The term “alkyl” as used herein is selected from straight-chain and branched saturated hydrocarbon groups containing 1 to 18 carbon atoms, such as 1 to 12, and even 1 to 6. A hydrocarbon group is meant. Examples of alkyl groups are methyl, ethyl, 1-propyl or n-propyl ("n-Pr"), 2-propyl or isopropyl ("i-Pr"), 1-butyl or n-butyl ("n-Bu "), 2-methyl-1-propyl or isobutyl (" i-Bu "), 1-methylpropyl or s-butyl (" s-Bu "), and 1,1-dimethylethyl or t-butyl (" t -Bu "). Other examples of alkyl groups are 1-pentyl (n-pentyl, --CH ₂ CH ₂ CH ₂ CH ₂ CH ₃ ), 2-pentyl (--CH (CH ₃ ) CH ₂ CH ₂ CH ₃ ), 3 -Pentyl (--CH (CH ₂ CH ₃ ) ₂ ), 2-methyl-2-butyl (--C (CH ₃ ) ₂ CH ₂ CH ₃ ), 3-methyl-2-butyl (--CH (CH _{3) CH (CH 3) 2} ), 3- methyl-1-butyl _{(--CH 2 CH 2 CH (CH} 3) 2), 2- methyl-1-butyl _{(--CH 2 CH (CH 3)} CH ₂ CH ₃ ), 1-hexyl (--CH ₂ CH ₂ CH ₂ CH ₂ CH ₂ CH ₃ ), 2-hexyl (--CH (CH ₃ ) CH ₂ CH ₂ CH ₂ CH ₃ ), 3-hexyl ( --CH (CH ₂ CH ₃ ) (CH ₂ CH ₂ CH ₃ )), 2-methyl-2-pentyl (--C (CH ₃ ) ₂ CH ₂ CH ₂ CH ₃ ), 3-methyl-2-pentyl (--CH (CH ₃ ) CH (CH ₃ ) CH ₂ CH ₃ ), 4-methyl-2-pentyl (--CH (CH ₃ ) CH ₂ CH (CH ₃ ) ₂ ), 3-methyl-3- Pentyl (--C (CH ₃ ) (CH ₂ CH ₃ ) ₂ ), 2-methyl-3-pentyl (--CH (CH ₂ CH ₃ ) CH (CH ₃ ) ₂ ), 2,3-dimethyl-2 -Butyl (--C (CH ₃ ) ₂ CH (CH ₃ ) ₂ ) and 3,3-dimethyl-2-butyl (--CH (CH ₃ ) C (CH ₃ ) ₃ groups may be selected.

The term “alkenyl” as used herein is selected from straight and branched hydrocarbon groups containing 2 to 18 carbon atoms, such as at least one C═C double bond and 2 to 6. A hydrocarbon group is meant. Examples of alkenyl groups include ethenyl or vinyl (--CH = CH _2), prop-1-enyl (--CH = CHCH _3), prop-2-enyl _{(--CH 2 CH = CH 2)} , 2- Methylprop-1-enyl, but-1-enyl, but-2-enyl, but-3-enyl, buta-1,3-dienyl, 2-methylbuta-1,3-diene, hexa-1-enyl, hexa- It can be selected from 2-enyl, hexa-3-enyl, hexa-4-enyl, and hexa-1,3-dienyl groups.

As used herein, the term “alkynyl” refers to a carbon selected from linear and branched hydrocarbon groups containing 2 to 18 carbon atoms, such as at least one C≡C triple bond and 2 to 6. It means a hydrogen group. Examples of alkynyl groups include ethynyl (—C≡CH), 1-propynyl (—C≡CCH ₃ ), 2-propynyl (propargyl, —CH ₂ C≡CH), 1-butynyl, 2-butynyl, and 3-butynyl group is included.

  The term “cycloalkyl” as used herein refers to a hydrocarbon selected from saturated and partially unsaturated cyclic hydrocarbon groups, including monocyclic and polycyclic (eg, bicyclic and tricyclic) groups. Means group. For example, a cycloalkyl group can contain 3 to 12 carbon atoms, such as 3 to 8, and even 3 to 6, 3 to 5, or 3 to 4, and so on. Further, for example, the cycloalkyl group may be selected from monocyclic groups containing 3 to 12 carbon atoms, such as 3 to 8, 3 to 6, and the like. Examples of monocyclic cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, 1-cyclopent-1-enyl, 1-cyclopent-2-enyl, 1-cyclopent-3-enyl, cyclohexyl, 1-cyclohex-1- Included are the enyl, 1-cyclohex-2-enyl, 1-cyclohex-3-enyl, cyclohexadienyl, cycloheptyl, cyclooctyl, cyclononyl, cyclodecyl, cycloundecyl, and cyclododecyl groups. Examples of bicyclic cycloalkyl groups include bicyclic rings selected from [4,4], [4,5], [5,5], [5,6] and [6,6] ring systems Or having 7 to 12 ring atoms arranged as a bridged bicyclic ring selected from or as bicyclo [2.2.1] heptane, bicyclo [2.2.2] octa, and bicyclo [3.2.2] nonane Things are included. The ring may be saturated, or may have at least one double bond (ie, partially unsaturated), but is not fully conjugated and not aromatic, aromatics are herein Defined in the document.

As used herein, the term “aryl” refers to a group selected from:
5- and 6-membered aromatic carbocycles such as phenyl;
A bicyclic ring system such as a 7 to 12 membered bicyclic ring system wherein at least one ring is carbocyclic and aromatic, for example selected from naphthalene, indane, and 1,2,3,4-tetrahydroquinoline A cyclic ring system; and a tricyclic ring system, such as a 10-15 membered tricyclic ring system, wherein at least one ring is carbocyclic and aromatic, eg, fluorene.

  For example, an aryl group is from a 5- to 7-membered heterocycle or a 5- and 6-membered aromatic carbocycle fused to a cycloalkyl that may contain at least one heteroatom selected from N, O, and S. Selected, provided that when the aromatic carbocycle is fused to a heterocycle, the point of attachment is on the aromatic carbocycle, and if the aromatic carbocycle is fused to a cycloalkyl group, the point of attachment is aromatic carbon It can be in a ring or a cycloalkyl group. A divalent group formed from a substituted benzene derivative and having a free valence at a ring atom is called a substituted phenylene group. By removing one hydrogen atom from a carbon atom having a free valence, a divalent group derived from a monovalent polycyclic hydrocarbon group whose name ends with “-yl” is the corresponding monovalent For example, a naphthyl group having two points of attachment is called naphthylidene. However, aryl does not encompass heteroaryl, which is defined separately below, or does not overlap in any manner. Thus, when one or more aromatic carbocycles are fused with an aromatic heterocycle, the resulting ring system is heteroaryl as defined herein and not aryl.

  As used herein, the term “halogen” or “halo” means F, Cl, Br or I.

As used herein, the term “heteroaryl” refers to a group selected from:
At least one heteroatom selected from N, O, and S, for example 1 to 4, or in some embodiments, 1 to 3 heteroatoms, with the remaining ring atoms being carbon; A 5- to 7-membered aromatic monocyclic ring;
At least one heteroatom selected from N, O, and S, for example 1 to 4, or in some embodiments, 1 to 3, or in other embodiments, 1 or 2 heteroatoms And the remaining ring atoms are carbon, an 8- to 12-membered bicyclic ring, wherein at least one ring is aromatic and at least one heteroatom is present in the aromatic ring; and
At least one heteroatom selected from N, O, and S, for example 1 to 4, or in some embodiments, 1 to 3, or in other embodiments, 1 or 2 heteroatoms Wherein the remaining ring atom is carbon, an 11 to 14 membered tricyclic ring, wherein at least one ring is aromatic and at least one heteroatom is present in the aromatic ring.

  For example, a heteroaryl group includes a 5 to 7 membered aromatic heterocycle fused to a 5 to 7 membered cycloalkyl ring. For such fused bicyclic heteroaryl ring systems in which only one of the rings contains at least one heteroatom, the point of attachment may be on an aromatic heterocycle or cycloalkyl ring.

  When the total number of S and O atoms in the heteroaryl group is 2 or more, these heteroatoms are not adjacent to one another. In some embodiments, the total number of S and O atoms in the heteroaryl group is 2 or less. In some embodiments, the total number of S and O atoms in the heteroaryl group is 1 or less.

  Examples of heteroaryl groups include pyridyl (such as 2-pyridyl, 3-pyridyl, or 4-pyridyl, numbered from the linking position assigned to priority 1), cinnolinyl, pyrazinyl, 2,4-pyrimidinyl, 3,5-pyrimidinyl, 2,4-imidazolyl, imidazopyridinyl, isoxazolyl, oxazolyl, thiazolyl, isothiazolyl, thiadiazolyl, tetrazolyl, thienyl, triazinyl, benzothienyl, furyl, benzofuryl, benzimidazolyl, indolyl, isoindolyl, indolinyl, intarinyl, Pyrazinyl, pyridazinyl, pyrrolyl, triazolyl, quinolinyl, isoquinolinyl, pyrazolyl, pyrrolopyridinyl (such as 1H-pyrrolo [2,3-b] pyridin-5-yl), pyrazolopyridinyl (1H-pyrazolo [3,4-b] Pyridine-5-yl), benzoxazo (Benzo [d] oxazol-6-yl, etc.), pteridinyl, purinyl, 1-oxa-2,3-diazolyl, 1-oxa-2,4-diazolyl, 1-oxa-2,5-diazolyl, 1- Oxa-3,4-diazolyl, 1-thia-2,3-diazolyl, 1-thia-2,4-diazolyl, 1-thia-2,5-diazolyl, 1-thia-3,4-diazolyl, flazanil, Benzofurazanyl, benzothiophenyl, benzothiazolyl, benzoxazolyl, quinazolinyl, quinoxalinyl, naphthyridinyl, furopyridinyl, benzothiazolyl (such as benzo [d] thiazol-6-yl), indazolyl (such as 1H-indazol-5-yl) and 5,6,7, This includes, but is not limited to, 8-tetrahydroisoquinoline.

  As used herein, the term “heterocyclic” or “heterocycle” or “heterocyclyl” is selected from oxygen, sulfur, and nitrogen, such as 1-4 heteroatoms, or even 1-3 4- to 12-membered monocyclic, bicyclic and tricyclic, saturated, containing at least one heteroatom plus at least one carbon atom, such as 1 or 2 heteroatoms And a ring selected from partially unsaturated rings. As used herein, “heterocycle” refers to at least one heterocycle selected from N, O, and S fused with a 5, 6, and / or 7-membered cycloalkyl, aromatic carbocycle or aromatic heterocycle. 5- to 7-membered heterocycle containing atoms, provided that when the heterocycle is fused with an aromatic carbocycle or aromatic heterocycle, the point of attachment is at the heterocycle and the heterocycle is fused with cycloalkyl In that case, the point of attachment also means a ring which can be a cycloalkyl or heterocycle. As used herein, “heterocycle” means an aliphatic spiro ring containing at least one heteroatom selected from N, O and S, provided that the point of attachment is in the heterocycle. The ring may be saturated or may have at least one double bond (ie, partially unsaturated). Heterocycles may be substituted with oxo. The point of attachment may be a carbon or heteroatom in the heterocycle. A heterocycle is not a heteroaryl as defined herein.

  Examples of heterocycles include 1-pyrrolidinyl, 2-pyrrolidinyl, 2,4-imidazolidinyl, 2,3-pyrazolidinyl, 1-piperidinyl, 2-piperidinyl (numbered from the linking position assigned priority 1), 3-piperidinyl, 4-piperidinyl, 2,5-piperazinyl, pyranyl, 2-morpholinyl, 3-morpholinyl, oxiranyl, aziridinyl, thiranyl, azetidinyl, oxetanyl, thietanyl, 1,2-dithietanyl, 1,3-dithietanyl, dihydropyri Dinyl, tetrahydropyridinyl, thiomorpholinyl, thioxanyl, piperazinyl, homopiperazinyl, homopiperidinyl, azepanyl, oxepanyl, thiepanyl, 1,4-oxathianyl, 1,4-dioxepanyl, 1,4-oxathiepanyl, 1,4-oxaazepanyl, 1, 4-dithiepanyl, 1,4-thiazepanyl and 1,4-diazepane 1,4-di Anil, 1,4-azathianyl, oxazepinyl, diazepinyl, thiazepinyl, dihydrothienyl, dihydropyranyl, dihydrofuranyl, tetrahydrofuranyl, tetrahydrothienyl, tetrahydropyranyl, tetrahydrothiopyranyl, 1-pyrrolinyl, 2-pyrrolinyl, 3- Pyrrolinyl, indolinyl, 2H-pyranyl, 4H-pyranyl, 1,4-dioxanyl, 1,3-dioxolanyl, pyrazolinyl, pyrazolidinyl, dithianyl, dithiolanyl, pyrazolidinyl, imidazolinyl, pyrimidinonyl, 1,1-dioxo-thiomorpholinyl, 3-azabicyclo [ 3.1.0] hexanyl, 3-azabicyclo [4.1.0] heptanyl and azabicyclo [2.2.2] hexanyl include, but are not limited to. Substituted heterocycles include ring systems substituted with one or more oxo moieties, such as piperidinyl N-oxide, morpholinyl-N-oxide, 1-oxo-1-thiomorpholinyl and 1,1-dioxo-1-thiomorpholinyl Is also included.

  As used herein, the term “fused ring” refers to a polycyclic ring system in which two rings share only two ring atoms and one bond, for example, a bicyclic or tricyclic ring system. To do. Examples of fused rings are arranged as bicyclic rings selected from the aforementioned [4,4], [4,5], [5,5], [5,6] and [6,6] ring systems. A fused bicyclic cycloalkyl ring such as those having 7 to 12 ring atoms; a fused bicyclic aryl ring such as the aforementioned 7 to 12 membered bicyclic aryl ring system; the aforementioned 10 to 15 membered Fused tricyclic aryl rings such as the above-mentioned tricyclic aryl ring systems; fused bicyclic heteroaryl rings such as the aforementioned 8- to 12-membered bicyclic heteroaryl rings; and the aforementioned 11- to 14-membered tricyclic hetero rings Fused tricyclic heteroaryl rings such as aryl rings; as well as fused bicyclic or tricyclic heterocyclyl rings as described above.

  The compounds described herein may contain asymmetric centers and may therefore exist as enantiomers. If the compounds described herein have more than one asymmetric center, they may also exist as diastereomers. Enantiomers and diastereomers fall into a broader class of stereoisomers. All possible stereoisomers, such as substantially pure resolved enantiomers, racemic mixtures thereof, and mixtures of diastereomers are intended to be included. All stereoisomers of the compounds disclosed herein and / or pharmaceutically acceptable salts thereof are intended to be included. Unless otherwise stated, references to one isomer apply to any possible isomer. Whenever an isomeric composition is not specified, all possible isomers are included.

  As used herein, the term “substantially pure” means that the desired stereoisomer is 35% or less, such as 30% or less, such as 25% or less, or even 20% or less. It is meant to include up to% by weight of any other stereoisomer. In some embodiments, the term “substantially pure” refers to any other stereoisomer in which the desired stereoisomer is 10% or less, such as 5% or less, such as 1% or less. It means to include.

  Where a compound described herein contains an olefinic double bond, unless specified otherwise, such a double bond will include both E and Z geometric isomers.

Some of the compounds described herein may exist with different points of hydrogen bonding, referred to as tautomers. For example, a carbonyl -CH ₂ C (O) - group compounds including (keto forms) may undergo tautomerism hydroxyl -CH = C (OH) - may generate a group (enol form). Both keto and enol forms are intended to be included individually and mixtures thereof where applicable.

  It may be advantageous to separate reaction products from one another and / or from starting materials. The desired product of each stage or series of stages is separated and / or purified (hereinafter separated) to the desired degree of homogeneity by techniques common in the art. Typically such separations include multiphase extraction, crystallization from a solvent or solvent mixture, distillation, sublimation, or chromatography. Chromatography includes, for example: reverse phase and normal phase; size exclusion; ion exchange; high pressure, medium pressure and low pressure liquid chromatography methods and instruments; small scale analysis; simulated moving bed (“SMB”) and preparative thin or thick layer chromatography And any number of methods, including small scale thin layer and flash chromatography techniques. Those skilled in the art will apply techniques most likely to achieve the desired separation.

  Diastereomeric mixtures can be separated into their individual diastereomers on the basis of their physicochemical differences by methods well known to those skilled in the art, such as chromatography and / or fractional crystallization. Enantiomers are converted to diastereomeric mixtures by reaction of the enantiomeric mixture with a suitable optically active compound (eg, a chiral auxiliary such as a chiral alcohol or Mosher acid chloride) to separate the diastereomers. The individual diastereomers can be separated by conversion (eg, hydrolysis) to the corresponding pure enantiomers. Enantiomers can also be separated by use of chiral HPLC column.

  Single stereoisomers, eg, substantially pure enantiomers, may be obtained by resolution of racemic mixtures using methods such as generating diastereomers with optically active resolving agents ( Eliel, E. and Wilen, S. Stereochemistry of Organic Compounds. New York: John Wiley & Sons, Inc., 1994; Lochmuller, CH, et al. '' Chromatographic resolution of enantiomers: Selective review. '' J. Chromatogr. , 113 (3) (1975): pp. 283-302). Racemic mixtures of the chiral compounds of the invention can be separated and isolated by any suitable method including: (1) Formation of ionic diastereomeric salts with chiral compounds and fractional crystallization or other Separation by methods, (2) formation of diastereomeric compounds with chiral derivatization reagents, separation of diastereomers, and conversion to pure stereoisomers, and (3) substantially pure or enriched stereoisomerism Direct separation of the body under chiral conditions. See Wainer, Irving W., Ed. Drug Stereochemistry: Analytical Methods and Pharmacology. New York: Marcel Dekker, Inc., 1993.

“Pharmaceutically acceptable salts” include, for example, salts with inorganic acids selected from hydrochloride, phosphate, diphosphate, hydrobromide, sulfate, sulfinate, and nitrate. And, for example, malate, maleate, fumarate, tartrate, succinate, citrate, lactate, methanesulfonate, p-toluenesulfonate, 2-hydroxyethylsulfonate An organic acid selected from alkanoates such as benzoates, salicylates, stearates, acetates, and salts with HOOC- (CH ₂ ) _n —COOH, wherein _n is selected from 0 to 4 But not limited thereto. Similarly, examples of pharmaceutically acceptable cations include, but are not limited to, sodium, potassium, calcium, aluminum, lithium, and ammonium.

  In addition, when the compounds disclosed herein are obtained as acid addition salts, the free base can be obtained by basifying a solution of the acid salt. Conversely, when the product is a free base, an addition salt, such as a pharmaceutically acceptable addition salt, is prepared according to the usual procedure for preparing acid addition salts from basic compounds and the free base is converted into a suitable organic solvent. And may be produced by treating the solution with an acid. Those skilled in the art will appreciate the various synthetic methods that can be used without undue experimentation to prepare non-toxic pharmaceutically acceptable addition salts.

  As defined herein, a “pharmaceutically acceptable salt thereof” refers to a salt of at least one compound of formula I, II, III, IV, and / or V, and a salt of an enantiomer, and / or It includes stereoisomeric salts of at least one compound of formula I, II, III, IV, and / or V, such as diastereomeric salts.

  “Treating”, “treating”, or “treatment” or “reduction” refers to at least one compound and / or at least one stereoisomer thereof and / or at least one thereof disclosed herein. It means that a pharmaceutically acceptable salt is administered to a subject who has, for example, cancer and is recognized as in need thereof.

  The term “effective amount” refers to a disease or disorder of interest of at least one compound disclosed herein and / or at least one stereoisomer thereof, and / or at least one pharmaceutically acceptable salt thereof. Means an amount effective to “treat” as defined above.

The term “at least one substituent” disclosed herein includes from 1 to 4 substituents, such as, for example, 1 to 3 and even 1 or 2. For example, as disclosed herein, “at least one substituent R ¹⁶ ” is selected from the list of R ¹⁶ described herein, such as 1 to 3 or even 1 or 2 or the like Of 1 to 4 substituents.

Provided is at least one compound selected from a compound of formula I, stereoisomers thereof, and pharmaceutically acceptable salts thereof:

Where
Q is selected from C and N;
W is selected from C and N;
X is selected from CH ₂ and O;
Y is selected from NR ¹² , O, and S;
Z is selected from O, S, NR ¹³ , CO, SO, SO ₂ , and CR ¹³ R ¹⁴ ;
R ¹ , R ² , R ³ , R ⁴ , R ⁵ , and R ⁶ may be the same or different and are each hydrogen, halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, Alkynyl, -CN, -NR ¹³ R ¹⁴ , -OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ ,- Selected from NR ¹³ CONR ¹⁴ R ¹⁵ , -NR ¹³ CO ₂ R ¹⁴ , -SO ₂ R ¹³ , -NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , -NR ¹³ SO ₂ R ¹⁴ , and -NR ¹³ SO ₂ aryl, Wherein alkyl, alkenyl, alkynyl, cycloalkyl, heteroaryl, aryl, and heterocyclyl may be substituted with at least one substituent R ¹⁶ or (R ¹ and R ² ), and / or (R ³ and R ^4), and / or (R ⁵ and R ^6), together with the ring to which they are attached, at least one Substituted with a substituent R ¹⁶ form a fused ring selected from heterocyclyl and heteroaryl ring; provided that when Q is N, if R ¹ is absent, and W is N, R ⁵ is not present;
R ⁷ is selected from hydrogen, halogen, alkyl, —O-alkyl, and —S-alkyl;
R ¹² is selected from hydrogen and alkyl;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings.

In some embodiments, X is CH _2.

  In some embodiments, X is O.

  In some embodiments, Y is NH and W is C.

  In some embodiments, Y is S and W is C.

  In some embodiments, Q is C.

In some embodiments, Q is N and R ¹ is absent.

  In some embodiments, Z is O.

In some embodiments, R ¹ and R ² are each hydrogen.

In some embodiments, R ³ and R ⁴ together with the ring to which they are attached are naphthyridinyl (eg, dihydronaphthyridinyl), pyrrolopyridinyl (eg, pyrrolo [2,3-b] pyridine-4- Yl), and a heterocyclic or heteroaryl ring such as purinyl, which may be substituted with at least one substituent R ¹⁶ such as oxo.

In some embodiments, R ³ and R ⁴ can be the same or different and each is hydrogen, —CONR ¹³ R ¹⁴ such as —CONHCH ₃ , and at least one substituent R ¹⁶ such as at least one haloalkyl. Selected from heteroaryl (eg, imidazole) optionally substituted with, wherein haloalkyl is, for example, —CF ₃ .

In some embodiments, R ⁵ and R ⁶ are taken together with the ring to which they are attached, benzimidazolyl (eg, 1H-benzo [d] imidazol-2-yl), and imidazopyridinyl (eg, 3H— Forming a fused ring that is a heteroaryl ring, such as imidazo [4,5-c] pyridin-2-yl), wherein the heteroaryl ring is a haloalkyl (eg, —CF ₃ ), alkyl (eg, methyl, tert -Butyl), halogen, CN, haloalkyloxy (eg OCF ₃ ), alkyloxy (eg methoxy), hydroxyl, and optionally substituted with at least one substituent R ¹⁶ such as phenyl.

In some embodiments, R ⁵ and R ⁶ may be the same or different and each is hydrogen, aryl such as phenyl, and haloalkyl (eg, —CF ₃ ), alkyl (eg, methyl, tert-butyl) , Halogen, CN, haloalkyloxy (eg OCF ₃ etc.), alkyloxy (eg methoxy), hydroxyl, and heteroaryl optionally substituted by at least one substituent R ¹⁶ such as phenyl (eg pyridyl) Selected from.

In some embodiments, at least one compound selected from a compound of formula (I), a stereoisomer thereof, and a pharmaceutically acceptable salt thereof is a compound of formula (II), a stereoisomer thereof: , And pharmaceutically acceptable salts thereof:
Where
Q is selected from C and N;
X is selected from CH ₂ and O;
Y is selected from NR ¹² , O, and S;
Z is selected from O, S, NR ¹³ , CO, SO, SO ₂ , and CR ¹³ R ¹⁴ ;
R ¹ , R ² , R ³ , R ⁴ , R ⁸ , R ⁹ , R ¹⁰ and R ¹¹ may be the same or different and are each hydrogen, halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, Heterocyclyl, heteroaryl, alkynyl, -CN, -NR ¹³ R ¹⁴ , -OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ , -NR ¹³ CONR ¹⁴ R ¹⁵ , -NR ¹³ CO ₂ R ¹⁴ , -SO ₂ R ¹³ , -NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , and -NR ¹³ SO ₂ R ¹⁴ Alkyl, alkenyl, alkynyl, cycloalkyl, heteroaryl, aryl, and heterocyclyl may each be substituted with at least one substituent R ¹⁶ , or (R ¹ and R ² ), and / or (R ³ and R ⁴ ), and / or (R ⁸ and R ⁹ ), and / or (R ⁹ and R ¹⁰ ), and / or (R ¹⁰ and R ¹¹ ) together with the ring to which they are attached form a fused ring selected from heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ ; When is N, R ¹ is absent;
R ⁷ is selected from hydrogen, halogen, alkyl, —O-alkyl, and —S-alkyl;
R ¹² is selected from hydrogen and alkyl;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings.

  In some embodiments, X of formula (II) is O.

In some embodiments, X of formula (II) is CH _2.

  In some embodiments, Y of formula (II) is S.

  In some embodiments, Z of formula (II) is O.

  In some embodiments, Q of formula (II) is C.

In some embodiments, Q of formula (II) is N and R ¹ of formula (II) is absent.

In some embodiments, R ³ and R ⁴ together with the ring to which they are attached are naphthyridinyl (eg, dihydronaphthyridinyl), pyrrolopyridinyl (eg, pyrrolo [2,3-b] pyridine-4- Yl), and a heterocyclic or heteroaryl ring such as purinyl, which may be substituted with at least one substituent R ¹⁶ such as oxo.

In some embodiments, R ¹ and R ² are each hydrogen.

In some embodiments, R ³ and R ⁴ can be the same or different and each is hydrogen, —CONR ¹³ R ¹⁴ such as —CONHCH ₃ , and at least one substituent R ¹⁶ such as at least one haloalkyl. Selected from heteroaryl (eg, imidazole) optionally substituted with, wherein haloalkyl is, for example, —CF ₃ .

In some embodiments, R ⁸ , R ⁹ , R ¹⁰ , and R ¹¹ in formula (II) can be the same or different and are each alkyl (eg, methyl, tert-butyl), hydrogen, haloalkyl ( For example, selected from —CF ₃ ), halogen, hydroxy, —CN, —O alkyl (eg, methoxy), and —O haloalkyl (eg, OCF ₃ ), and aryl (eg, phenyl).

In some embodiments, at least one compound selected from a compound of formula (I), a stereoisomer thereof, and a pharmaceutically acceptable salt thereof is a compound of formula (III), a stereoisomer thereof, and Selected from its pharmaceutically acceptable salts:
Where
X is selected from CH ₂ and O;
Z is selected from O, S, NR ¹³ , CO, SO, SO ₂ , and CR ¹³ R ¹⁴ ;
A is _{-CH 2 -, - CH 2 CH} 2 -, - CH = CH -, = CH -, - NR 13 -, - CH 2 -O -, - O-, and is selected from -S-;
M is selected from = CH- and -C (O)-; or M is absent;
R ¹ , R ² , R ⁸ , R ⁹ , R ¹⁰ and R ¹¹ may be the same or different and are each hydrogen, halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, alkynyl , -CN, -NR ¹³ R ¹⁴ , -OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ , -NR ¹³ CONR ¹⁴ R ¹⁵ , —NR ¹³ CO ₂ R ¹⁴ , —SO ₂ R ¹³ , —SO ₂ aryl, —NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , and —NR ¹³ SO ₂ R ¹⁴ , where alkyl , Alkenyl, alkynyl, cycloalkyl, heteroaryl, aryl, and heterocyclyl may each be substituted with at least one substituent R ¹⁶ ,
Or (R ¹ and R ² ) and / or (R ⁸ and R ⁹ ) and / or (R ⁹ and R ¹⁰ ) and / or (R ¹⁰ and R ¹¹ ) are the rings to which they are attached Together with a fused ring selected from heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ ;
R ¹² is selected from hydrogen and alkyl;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings.

In some embodiments, the AM of formula (III) is —CH ₂ —CH ₂ —C (O) —.

  In some embodiments, A-M of formula (III) is —CH═CH—.

In some embodiments, the AM of formula (III) is —CH ₂ —OC (O) —.

In some embodiments, R ¹² of formula (III) is H.

  In some embodiments, X of formula (III) is O.

In some embodiments, X of formula (III) is CH _2.

  In some embodiments, Z of formula (III) is O.

In some embodiments, R ⁸ , R ⁹ , R ¹⁰ , and R ¹¹ in formula (III) can be the same or different and are each alkyl (eg, methyl, tert-butyl), hydrogen, haloalkyl ( For example, selected from —CF ₃ ), halogen, hydroxy, —CN, —O alkyl (eg, methoxy), —O haloalkyl (eg, OCF ₃ ), and aryl (eg, phenyl).

In some embodiments, at least one compound selected from a compound of formula (I), a stereoisomer thereof, and a pharmaceutically acceptable salt thereof is a compound of formula (IV), a stereoisomer thereof, and Selected from its pharmaceutically acceptable salts:
Where
B is selected from CH ₂ , O, and NR ¹³ ;
X is selected from CH ₂ and O;
R ¹ , R ² , R ⁸ , R ⁹ , R ¹⁰ and R ¹¹ may be the same or different and are each hydrogen, halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, alkynyl , -CN, -NR ¹³ R ¹⁴ , -OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ , -NR ¹³ CONR ¹⁴ R ¹⁵ , —NR ¹³ CO ₂ R ¹⁴ , —SO ₂ R ¹³ , —SO ₂ aryl, —NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , and —NR ¹³ SO ₂ R ¹⁴ , where alkyl , Alkenyl, alkynyl, cycloalkyl, heteroaryl, aryl, and heterocyclyl may each be substituted with at least one substituent R ¹⁶ or (R ¹ and R ² ) and / or (R ⁸ and R ⁹ ), and / or (R ⁹ and R ^10), and / or (R ¹⁰ and R ^11), they are sintered With to that ring, form a fused ring selected from at least one of which may be substituted with a substituent R ¹⁶ heterocyclyl and heteroaryl ring;
R ¹² is selected from hydrogen and alkyl;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings.

In some embodiments, R ¹ and R ² can be the same or different and are each selected from hydrogen, alkyl, and halo.

In some embodiments, R ¹² of formula (IV) is H.

  In some embodiments, X of formula (IV) is O.

In some embodiments, X of formula (IV) is CH _2.

In some embodiments, R ⁸ , R ⁹ , R ¹⁰ , and R ¹¹ in formula (IV) can be the same or different and are each alkyl (eg, methyl, tert-butyl), hydrogen, haloalkyl ( For example, selected from —CF ₃ ), halogen, hydroxy, —CN, —O alkyl (eg, methoxy), —O haloalkyl (eg, OCF ₃ ), and aryl (eg, phenyl).

In some embodiments, at least one compound selected from a compound of formula (I), a stereoisomer thereof, and a pharmaceutically acceptable salt thereof is a compound of formula (V), a stereoisomer thereof, and Selected from its pharmaceutically acceptable salts:
Where
X is selected from CH ₂ and O;
R ⁸ , R ⁹ , R ¹⁰ and R ¹¹ may be the same or different and are each hydrogen, halogen, alkyl, alkenyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, alkynyl, -NR ¹³ R ¹⁴ ,- OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ , -NR ¹³ CONR ¹⁴ R ¹⁵ , -NR ¹³ CO ₂ R ¹⁴ , —SO ₂ R ¹³ , —SO ₂ aryl, —NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , and —NR ¹³ SO ₂ R ¹⁴ , wherein alkyl, alkenyl, alkynyl, cycloalkyl, heteroaryl, Aryl and heterocyclyl may each be substituted with at least one substituent R ¹⁶ or (R ⁸ and R ⁹ ) and / or (R ⁹ and R ¹⁰ ) and / or (R ¹⁰ and R ¹¹ ) Is substituted with at least one substituent R ¹⁶ together with the ring to which they are attached Forming a fused ring selected from optionally heterocyclyl and heteroaryl rings;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings.

  In some embodiments, at least one compound of formula (V) is optically pure.

  In some embodiments, X of formula (V) is O.

In some embodiments, X of formula (V) is CH _2.

In some embodiments, R ⁸ , R ⁹ , R ¹⁰ , and R ¹¹ in formula (III) can be the same or different and are each alkyl (eg, methyl, tert-butyl), hydrogen, haloalkyl ( For example, selected from —CF ₃ ), halogen, hydroxy, —CN, —O alkyl (eg, methoxy), —O haloalkyl (eg, OCF ₃ ), and aryl (eg, phenyl).

Also provided herein is at least one compound selected from the following compounds, stereoisomers thereof, and pharmaceutically acceptable salts thereof.

Also provided herein is at least one compound selected from the following compounds that exhibit the following stereochemistry:

  The compounds disclosed herein and / or their pharmaceutically acceptable salts can be synthesized from commercially available starting materials in conjunction with the disclosure herein. The following schemes illustrate some methods for preparing the compounds disclosed herein.

Scheme I
Pr = protecting group;
PrO = protected hydroxy group;
Alk = alkyl group;
Halo = halogen.

In this scheme, the hydroxyl group of a commercially available hydroxyl benzofuran or hydroxyl indene of formula 1 is replaced with a hydroxyl protecting group (methyl, ethyl, isopropyl, benzyl, p-methoxybenzyl, trityl, methoxymethyl, tetrahydropyranylacetyl, benzoate, trimethylsilyl, Protection of Formula 2 by protection with triethylsilyl, tri-isopropylsilyl, tert-butyldimethylsilyl or tert-butyldiphenylsilyl, and even benzyl from benzyl bromide and tert-butyldiphenylsilyl from TBSCl) Hydroxybenzofuran or hydroxyindene is provided. A compound of formula 2 reacts with an alkyl diazoacetate (such as ethyl diazoacetate) in the presence of Rh or Cu catalyst to provide a cyclopropane derivative of formula 3. Chiral derivatives of formula 3 may be obtained using chiral catalysts and chiral amino alcohols formed in situ from Cu (OOCCF ₃ ) 2 or using commercially available chiral Rh catalysts. The compound of formula 3 is deprotected as described above to provide the phenol derivative of formula 4 (eg, the TBS protecting group may be removed by treatment with hydrogen fluoride pyridine). The resulting phenol derivative of formula 4 is reacted with a haloheteroaryl derivative of formula 5 (such as a fluoro-substituted heteroaryl derivative of formula 5) to provide a compound of formula 6, followed by a base such as sodium hydroxide. Is hydrolyzed to the free acid of formula 7. Further coupling and cyclization of acid 7 is achieved under standard conditions known in the art to provide compounds of formula I.

  A method of treating a cancer responsive to inhibition of Raf kinase, wherein a subject such as a mammal or a human in need of treatment for cancer is treated with a compound of formula (I) (eg, formula ( A method comprising administering an effective amount of at least one compound selected from the compounds of II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof. Are also provided herein.

  At least one compound selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof is May be used alone or in combination with at least one other therapeutic agent for treatment. In some embodiments, selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof. The at least one compound can also be used in combination with at least one additional therapeutic agent. The at least one additional therapeutic agent can be selected from, for example, anti-hyperproliferative agents, anti-cancer agents, and chemotherapeutic agents. At least one compound and / or at least one pharmaceutically acceptable salt disclosed herein may be administered with at least one other therapeutic agent, in a single dosage form, or as separate dosage forms. May be. When administered as separate dosage forms, the at least one other therapeutic agent is prior to, concurrent with, or after administration of at least one compound and / or at least one pharmaceutically acceptable salt disclosed herein. It may be administered. A “chemotherapeutic agent” is a chemical compound useful in the treatment of cancer, regardless of mechanism of action. Chemotherapeutic agents include compounds used in “targeted therapy” and conventional chemotherapy. Suitable chemotherapeutic agents can be selected, for example, from: agents that induce apoptosis; polynucleotides (eg, ribozymes); polypeptides (eg, enzymes); drugs; biological mimetics; alkaloids; Antitumor antibiotics; antimetabolites; hormones; platinum compounds; monoclonal antibodies conjugated to anticancer drugs, toxins, and / or radionuclides; biological response modifiers (eg, IFN-a Interleukins such as interferon and IL-2); adoptive immunotherapy agents; hematopoietic growth factors; agents that induce tumor cell differentiation (eg, all-trans retinoic acid); gene therapy reagents; antisense therapy reagents and nucleotides; tumor vaccines As well as angiogenesis inhibitors.

  Examples of chemotherapeutic agents include erlotinib (TARCEVA®, Genentech / OSI Pharm.); Bortezomib (VELCADE®, Millennium Pharm.); Fulvestrant (FASLODEX®, AstraZeneca); sunitinib (SUTENT®, Pfizer); letrozole (FEMARA®, Novartis); imatinib mesylate (GLEEVEC®, Novartis); PTK787 / ZK 222584 (Novartis); oxaliplatin (Eloxatin®) ), Sanofi); 5-FU (5-fluorouracil); leucovorin; rapamycin (sirolimus, RAPAMUNE®, Wyeth); rapatinib (TYKERB®, GSK572016, Glaxo Smith Kline); lonafarnib (SCH 66336); Sorafenib (NEXAVAR®, Bayer); irinotecan (CAMPTOSAR®, Pfizer) and gefitinib (IRESSA®, AstraZeneca); AG1478, AG1571 (SU 5271, Sugen); And alkylating agents such as CYTOXAN® cyclosphosphamide; alkyl sulfonates such as busulfan, improsulfan and piperosulfan; benzodopa, carbodopa, meturedopa, and uredopa Aziridines such as; altretamine, triethylenemelamine, triethylenephosphoramide, triethylenethiophosphoramide, and trimethylomelamine; and ethyleneimine and methylamelamine; acetogenin (such as bratasin and brataginone); camptothecin (such as synthetic analog topotecan) Bryostatin; calistatin; CC-1065 and its adzelesin, calzeresin and bizelesin synthetic analogues; cryptophycin (cryptoficin 1 and Toficin 8); Dolastatin; Duocarmycin and its synthetic analogs such as KW-2189 and CB1-TM1; Eluterobin; Panclatistatin; Sarcodictin; Spongestatin; Chlorambucil, Chlomaphazine, Chlorophosphamide, Nitrogen mustards such as estramustine, ifosfamide, mechlorethamine, mechloretamine oxide hydrochloride, melphalan, nobubiquin, phenesterine, prednisomus, trophosphamide, uracil mustard; Nitrosurea; enediyne antibiotics such as calicheamicin gamma 1I and calicheamicin omega I1 Antibiotics such as calicheamicin (Angew Chem. Intl. Ed. Engl. (1994) 33: 183-186); Dynemicins such as Dynemicin A; Bisphosphonates such as Clodronate; Esperamicins; and the neocardinostatin chromophore and related Chromoprotein enediyne antibiotic chromophore, aclacinomycin, actinomycin, authramycin, azaserine, bleomycin, cactinomycin, carabicin, carminomycin, cardinophylline, chromomycin, dactino Mycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, ADRIAMYCIN® (doxorubicin), morpholino-doxorubicin, cyanomorpholino-doxorubicin, 2-pyrrolino-doxorubicin and deoxyxorubicin), Mitomycin such as pyrubicin, esorubicin, idarubicin, marcelomycin, mitomycin C, mycophenolic acid, nogaramycin, olivomycin, peplomycin, porphyromycin, puromycin, keramycin (quelamycin), rhodorubicin, streptonigrin, streptozocin, Tubercidine, ubenimex, dinostatin, zorubicin; antimetabolites such as methotrexate and 5-fluorouracil (5-FU); folic acid analogs such as denopterine, methotrexate, pteropterin, trimetrexate; fludarabine, 6-mercaptopurine, thiampurine, thioguanine, etc. Purine analogues of: ancitabine, azacitidine, 6-azauridine, carmofur, cytarabine, dideoxyuridine, doxif Pyrimidine analogues such as lysine, enocitabine, and furoxyuridine; androgens such as carsterone, drmostanolone propionate, epithiostanol, mepithiostan, and test lactone; anti-adrenal agents such as aminoglutethimide, mitotane, and trilostane; frolinic acid, etc. Folate supplements; acegraton; aldophosphamide glycosides; aminolevulinic acid; eniluracil; amsacrine; vesturacil; bisantrene; edatraxate; defofamine; demecorsin; diazicon; elformithine; Eliptynium; Epothilone; Etogluside; Gallium nitrate; Hydroxyurea; Lentinan; Lonidamine; Maytansinoids such as maytansine and ansamitocin; Toguazone; Mitoxantrone; mopidanmol; nitraerine; pentostatin; phenmet; pirarubicin; rosoxantrone; podophyllic acid; 2-ethylhydrazide; procarbazine; PSK® polysaccharide complex (JHS Natural Products, Eugene, Razoxan; Rhizoxin; Schizophyllan; Spirogermanium; Tenuazonic acid; Triadicon; 2,2 ', 2' '-Trichlorotriethylamine; Trichothecene (T-2 toxin, verracurin A, Loridin A and Anguidine, etc.); Urethane; vindesine; dacarbazine; mannomustine; mitoblonitol; mitracitol; pipobroman; gacytosine; arabinoside (“Ara-C”); cyclophosphamide; thiotepa; taxoids such as TAXOL® (paclita) Bristol-Myers Squibb Oncology, Princeton, NJ), ABRAXANE® (not including Cremophor), paclitaxel albumin modified nanoparticle formulation (American Pharmaceutical Partners, Schaumberg, Ill.), And TAXOTERE® ( Rhone-Poulenc Rorer, Antony, France; chlorambucil; GEMZAR® (gemcitabine); 6-thioguanine; mercaptopurine; methotrexate; platinum analogs such as cisplatin and carboplatin; vinblastine; etoposide (VP- 16); Ifosfamide; Mitoxantrone; Vincristine; NAVELBINE (R) (vinorelbine); Novantrone; Teniposide; Edatrexate; Daunomycin; Aminopterin; Capecitabine (XELODA (R)); Ibandronate; CPT-11; Topoisomerase inhibition Agent RFS2000; Le Oro methyl ornithine (DMFO); retinoids such as retinoic acid; and any pharmaceutically acceptable salts of the above, include acid and derivatives.

A “chemotherapeutic agent” can also be selected, for example, from: (i) an anti-hormonal agent that acts to modulate or inhibit hormonal action on tumors, such as tamoxifen (NOLVADEX®; tamoxifen citrate) And estrogen and selective estrogens, including raloxifene, droloxifene, 4-hydroxy tamoxifen, trioxyphene, keoxifene, LY117018, onapristone, and FARESTON® (toremifine citrate) Receptor modulators (SERM); (ii) aromatase inhibitors that inhibit aromatase, an enzyme that regulates estrogen production in the adrenal glands, such as 4 (5) -imidazole, aminoglutethimide, MEGASE® (acetic acid Megestrol), AROMASIN (registered trademark) (Exemesta) Pfizer, formestanie, fadrozole, RIVISOR® (borozole), FEMARA® (Letolezole; Novartis), and ARIMIDEX® (anastrozole; AstraZeneca); (iii) Antiandrogens, such as flutamide, nilutamide, bicalutamide, leuprolide, and goserelin; and toloxacitabine (1,3-dioxolane nucleoside cytosine analog); (iv) protein kinase inhibitors; (v) lipid kinase inhibitors; Sense oligonucleotides, such as those that inhibit the expression of genes in signal transduction pathways involved in abnormal cell growth, such as PKC-alpha, Ralf and H-Ras; (vii) ribozymes, such as VEGF expression inhibitors (eg, , ANGIOZYME®) and HER ² expression inhibitors; (viii) vaccines, eg For example, gene therapy vaccines such as ALLOVECTIN®, LEUVECTIN®, and VAXID®; PROLEUKIN® rIL-2; topoisomerase 1 inhibitors such as LURTOTECAN®; ABARELIX (Ix) rmRH; (ix) anti-angiogenic agents such as bevacizumab (AVASTIN®, Genentech); and (x) pharmaceutically acceptable salts, acids and derivatives of any of the above.

  “Chemotherapeutic agents” include, for example, therapeutic antibodies such as alemtuzumab (Campath), bevacizumab (AVASTIN®, Genentech); cetuximab (ERBITUX®, Imclone); panitumumab (VECTIBIX®), Amgen), Rituximab (RITUXAN®, Genentech / Biogen Idec), Pertuzumab (OMNITARG®, 2C4, Genentech), Trastuzumab (HERCEPTIN®, Genentech), Tositumomab (Bexxar, Corixia), and antibodies It can also be selected from the drug complex, gemtuzumab ozogamicin (MYLOTARG®, Wyeth).

  At least one compound selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof; A humanized monoclonal antibody having therapeutic potential as a chemotherapeutic agent in combination may be selected, for example, from: alemtuzumab, apolizumab, acelizumab, atorizumab, bapineuzumab, bevacizumab, bivacuzumab Mertansine, Cantuzumab Mertansine, Cedelizumab, Sertolizumab Pegor, Cidfusituzumab, Cidtuzumab, Daclizumab, Eculizumab, Efalizumab, Epratuzumab, Erlizumab, Fontrizumab, Felbizumab Zome Inotuzumab ozogamicin Ipilimumab, labetuzumab, lintuzumab, matuzumab, mepolizumab, motavizumab, Motobizumabu (motovizumab), natalizumab, nimotuzumab, Norobizumabu (nolovizumab), Nubabizumabu (numavizumab), ocrelizumab, omalizumab, palivizumab, Pasukorizumabu, Pekufushitsuzumabu (pecfusituzumab), Pekutsuzumabu (pectuzumab), pertuzumab , Pexelizumab, ralivizumab (ralivizumab), ranibizumab, reslivizumab (reslivizumab), resrizumab, resyzizumab (resyvizumab), robelizumab (rovelizumab), rupizumab (ruplizumab), tiblizumab tadocizumab), tarizumab, tefivazumab, tocilizumab, tralizumab, Trastuzumab, tucotuzumab selmoloikin, tucusituzumab, umavizumab, urtoxazumab, and vizilizumab.

  At least one compound selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof; Also provided herein is a composition comprising at least one pharmaceutically acceptable carrier.

  At least one compound selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof, The containing composition can be administered in a variety of known ways, such as oral, topical, rectal, parenteral, inhalation spray, or an implanted reservoir, although the most suitable route in any given case is specific The host and the nature and severity of the condition to which the active ingredient is being administered. The term “parenteral” as used herein refers to subcutaneous, intradermal, intravenous, intramuscular, intraarticular, intraarterial, intrasynovial, intrasternal, intrathecal, intralesional and intracranial injection. Or including injection techniques. The compositions disclosed herein are conveniently presented in unit dosage form and may be prepared by any method known in the art.

  At least one compound selected from Formula (I) (eg, Formula (II), (III), (IV) and (V)), its stereoisomers, and pharmaceutically acceptable salts thereof is a capsule It can be administered orally in solid dosage forms such as drugs, tablets, troches, dragees, granules and powders, or liquid dosage forms such as elixirs, syrups, emulsions, dispersions and suspensions. Selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers, and pharmaceutically acceptable salts thereof disclosed herein. The at least one compound may also be administered parenterally in sterile liquid dosage forms such as dispersions, suspensions or solutions. Ointments, creams, drops, transdermal patches or powders for topical administration, ophthalmic solutions or suspension formulations for administration to the eye, ie, aerosol sprays or powders for inhalation or intranasal administration as eye drops As a composition or as a cream, ointment, spray or suppository for rectal or vaginal administration, the compounds of formula (I) disclosed herein (eg, formulas (II), (III), (IV) and (V) )), Its stereoisomers, and its pharmaceutically acceptable salts, other dosage forms can also be used to administer at least one compound.

  Also used is a gelatin capsule comprising at least one compound disclosed herein and / or at least one pharmaceutically acceptable salt thereof and a powder carrier such as lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, etc. Can do. Similar diluents can be used to make compressed tablets. Both tablets and capsules can be manufactured as sustained release products to provide for continuous release of medication over a period of time. Compressed tablets can be sugar coated or film coated to mask any unpleasant taste and protect the tablet from the environment, or enteric coated for selective disintegration in the gastrointestinal tract .

  Liquid dosage forms for oral administration can further comprise at least one agent selected from colorants and flavors to enhance patient acceptance.

  In general, water, suitable oils, saline, aqueous dextrose (glucose), and related sugar solutions and glycols such as propylene glycol or polyethylene glycol may be examples of suitable carriers for parenteral solutions. Solutions for parenteral administration may contain a water-soluble salt of at least one compound described herein, at least one suitable stabilizer, and, if necessary, at least one buffering substance. . Antioxidants such as sodium bisulfite, sodium sulfite, or ascorbic acid may be examples of suitable stabilizers, either alone or in combination. Citric acid and its salts and sodium EDTA can also be used as examples of suitable stabilizers. In addition, parenteral solutions can further comprise at least one preservative selected from, for example, benzalkonium chloride, methyl and propylparaben, and chlorobutanol.

  A pharmaceutically acceptable carrier is, for example, a carrier that is compatible with the active ingredient of the composition (and can stabilize the active ingredient in some embodiments) and is not harmful to the subject being treated. Selected from. For example, solubilizers such as cyclodextrins (which may form certain, more soluble complexes with at least one compound and / or at least one pharmaceutically acceptable salt disclosed herein) Can be used as pharmaceutical excipients for the delivery of active ingredients. Examples of other carriers include colloidal silicon dioxide, magnesium stearate, cellulose, sodium lauryl sulfate, and dyes such as D & C Yellow # 10. Suitable pharmaceutically acceptable carriers are described in Remington's Pharmaceutical Sciences, A. Osol, a standard reference text in the art.

  Selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers, and pharmaceutically acceptable salts thereof disclosed herein. The at least one compound can be further tested by an in vivo assay for efficacy in treating cancer. For example, at least one compound disclosed herein and / or at least one pharmaceutically acceptable salt thereof is administered to an animal having cancer (eg, a mouse model) and its therapeutic efficacy is evaluated. can do. A positive result in one or more of such tests is sufficient to increase the treasury of scientific knowledge and is therefore sufficient to demonstrate the utility of the tested compounds and / or salts. Based on the results, an appropriate dosage range and administration route for an animal such as a human can also be determined.

  For administration by inhalation, the compounds of formula (I) disclosed herein (eg, formulas (II), (III), (IV) and (V)), their stereoisomers, and pharmaceutically At least one compound selected from acceptable salts may be conveniently delivered from a compressed pack or nebulizer in the form of an aerosol propellant. Selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers, and pharmaceutically acceptable salts thereof disclosed herein. The at least one compound may be delivered as an optionally formulated powder and the powder composition may be inhaled utilizing an insufflated powder inhaler. One exemplary delivery system for inhalation can be a metered dose inhalation (MDI) aerosol, which is disclosed in Formula (I) (eg, Formula (II), (III), (IV) and ( At least one suitable propellant selected from, for example, fluorinated hydrocarbons and hydrocarbons, of at least one compound selected from the compounds of V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof It may be formulated as a suspension or solution therein.

  For administration to the eye, the ophthalmic formulation comprises a compound of formula (I) disclosed herein (eg, formulas (II), (III), (IV) and (V)), its stereoisomers And in at least one compound selected from pharmaceutically acceptable salts thereof in a suitable ophthalmic medium such that the compound continues to contact the ocular surface for a sufficient period of time to allow the compound to penetrate the cornea and internal regions of the eye. A suitable weight percentage of a compound of formula (I) disclosed herein (eg, formulas (II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable thereof Formulation may be made with a solution or suspension of at least one compound selected from the salts to be prepared.

  Selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers, and pharmaceutically acceptable salts thereof disclosed herein. Pharmaceutical dosage forms useful for administration of at least one compound include, but are not limited to, gelatin hard and soft capsules, tablets, parenteral injections, and oral suspensions. Absent.

  The dose to be administered will depend on factors such as the age, health and weight of the recipient, the extent of the disease, the type of combination treatment, the frequency of treatment, and the nature of the desired effect. Generally, the daily dose of active ingredient can vary, for example, from 0.1 to 2000 milligrams per day. For example, 10-500 milligrams a day may be effective one or more times to obtain the desired result.

  In some embodiments, standard two-part gelatin hard capsules are each, for example, powders of formula (I) (eg, formulas (II), (III), (IV) and (V) disclosed herein. )) Compound, its stereoisomer, and at least one compound selected from pharmaceutically acceptable salts thereof, 100 mg, lactose 150 mg, cellulose 50 mg, and magnesium stearate 6 mg A number of unit capsules can be prepared.

  In some embodiments, selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof. Preparing a mixture of digestible oils such as soybean oil, cottonseed oil or olive oil of at least one compound and injecting it into gelatin by a positive displacement pump to form a gelatin soft capsule containing 100 milligrams of the active ingredient it can. The capsule is washed and dried.

  In some embodiments, the dosage unit is, for example, a compound of formula (I) (eg, formula (II), (III), (IV) and (V)), a stereoisomer thereof, and a pharmaceutically acceptable salt thereof. By normal procedures to include 100 mg of at least one compound selected from the salts to be prepared, 0.2 mg of colloidal silicon dioxide, 5 mg of magnesium stearate, 275 mg of microcrystalline cellulose, 11 mg of starch and 98.8 mg of lactose, A number of tablets can be prepared. Appropriate coatings may be applied to increase palatability or delay absorption.

  In some embodiments, a parenteral composition suitable for administration by injection comprises 1.5% by weight of at least one compound disclosed herein and / or its at least one enantiomer, dia, in 10% by volume propylene glycol. It can be prepared by stirring a stereomer or a pharmaceutically acceptable salt. Sterilize the solution to the desired volume with water for injection.

  In some embodiments, aqueous suspensions can be prepared for oral administration. For example, at least one selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof 5 milliliters each of an aqueous suspension containing 100 milligrams of compound microparticles, 100 milligrams of sodium carboxymethylcellulose, 5 milligrams of sodium benzoate, 1.0 grams of sorbitol solution USP, and 0.025 milliliters of vanillin can be used.

  At least one compound selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers thereof, and pharmaceutically acceptable salts thereof, The same dosage form can generally be used when administered stepwise or together with at least one other therapeutic agent. When drugs are administered in physical combination, the dosage form and route of administration should be selected according to the compatibility of the combined drugs. Thus, the term “simultaneous administration” is understood to include administration of at least two agents simultaneously or sequentially or as a combination of fixed doses of at least two active ingredients.

  Selected from compounds of formula (I) (eg, formulas (II), (III), (IV) and (V)), stereoisomers, and pharmaceutically acceptable salts thereof disclosed herein. At least one second active ingredient selected as the sole active ingredient or other active ingredients known to be useful, for example, in treating cancer in a patient Can be administered in combination.

  The following examples are intended to be purely illustrative and should not be considered limiting in any way. Efforts have been made to ensure accuracy with respect to numbers used (eg amounts, temperature, etc.) but some experimental errors and deviations will be included. Unless otherwise noted, temperatures are in degrees Celsius. Reagents were purchased from commercial suppliers such as Sigma-Aldrich, Alfa Aesar, or TCI and used without further purification unless otherwise noted.

  Unless indicated otherwise, the reactions shown below are carried out under a positive pressure of nitrogen or argon, or in an anhydrous solvent, using a drying tube; the reaction flask is equipped with a rubber stopper for introducing the substrate and reagents from a syringe. The glassware was dried in a dryer and / or heat dried.

  Unless otherwise stated, column chromatography purification is performed on a Biotage system (manufacturer: Dyax Corporation) or silica SepPak cartridge (Waters) with a silica gel column, or using a silica gel cartridge pre-packed with a Teledyne Isco Combiflash purification system. I went.

¹ H NMR spectra were recorded on a Varian instrument operating at 400 MHz. ¹ H-NMR spectrum is CDCl ₃ , CD ₂ Cl ₂ , CD ₃ OD, D ₂ O, d ₆ -DMSO, d ₆ -acetone or (CD ₃ ) ₂ CO as solvent and tetramethylsilane (0.00 ppm as reference standard) ) Or residual solvent (CDCl ₃ : 7.25 ppm; CD ₃ OD: 3.31 ppm; D ₂ O: 4.79 ppm; d ₆ -DMSO: 2.50 ppm; d ₆ -acetone: 2.05; (CD ₃ ) ₂ CO: 2.05) Obtained. When reporting peak multiplicity, the following abbreviations are used: s (single line), d (double line), t (triple line), q (quadruple line), qn (quintet line), sx (six Double line), m (multiple line), br (wide), dd (double double line), dt (triple double line). If a coupling constant is indicated, report it in hertz (Hz). All compound names except reagents were created with ChemDraw version 12.0.

In the examples below, the following abbreviations are used.
AcOH acetic acid
Aq Aqueous saline NaCl saturated aqueous solution
Bn benzyl
BnBr Benzyl bromide
CH ₂ Cl ₂ dichloromethane
DMF N, N-dimethylformamide
Dppf 1,1 ''-bis (diphenylphosphino) ferrocene
DBU 1,8-diazabicyclo [5.4.0] undec-7-ene
DIEA or DIPEA N, N-diisopropylethylamine
DMAP 4-N, N-dimethylaminopyridine
DMF N, N-dimethylformamide
DMSO Dimethyl sulfoxide
EtOAc ethyl acetate
EtOH ethanol
Et ₂ O or ether diethyl ether
G grams
h or hr time
HATU 2- (1H-7-azabenzotriazol-1-yl) -1,1,3,3-tetramethyluronium hexafluorophosphate methanaminium
HCl hydrochloric acid
HPLC high performance liquid chromatography
IPA 2-propanol
i-PrOH Isopropyl alcohol
Mg milligram
mL milliliter
Mmol mmol
MeCN Acetonitrile
MeOH methanol
Min minutes
ms or MS mass spectrum
Na ₂ SO ₄ sodium sulfate
PE petroleum ether
PPA polyphosphate
Rt retention time
Rt or rt Room temperature
TBAF Tetrabutylammonium fluoride
TBSCl tert-butyldimethylsilyl chloride
TFA trifluoroacetic acid
THF tetrahydrofuran
TLC thin layer chromatography μL microliter

Example 1: Synthesis of compounds 1.1-1.3 Compound 1.1

Step A: 6- (Benzyloxy) -2,3-dihydroinden-1-one
Stir a solution of 6-hydroxy-2,3-dihydroinden-1-one (30 g, 0.203 mol) in DMF (300 mL) to which K ₂ CO ₃ (70 g, 0.507 mol) is added followed by BnBr (38.2 g, 0.225 mol) was added dropwise at 0 ° C. The mixture was returned to ambient temperature and stirred for 20 hours. The mixture was diluted with EtOAc (500 mL) and then filtered to remove solids. The filtrate was washed with brine (200 mL × 5), dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The resulting solid was recrystallized in ether to give the title compound (44.5 g, 92%) as a white solid.

Step B: 6- (Benzyloxy) -2,3-dihydro-1H-inden-1-ol
A solution of the product from Step A (44 g, 0.185 mol) in MeOH (500 mL) was stirred and to this was added NaBH ₄ (7.1 g, 0.186 mol) in portions at 0 ° C. The mixture was returned to ambient temperature and stirred for 20 hours. The reaction mixture was concentrated and 200 mL of aqueous NaOH (20%) was added. The resulting mixture was extracted with EtOAc (200 mL × 3). The combined organic extracts were washed with brine (200 mL × 3), dried and concentrated to give the title compound (32.0 g, 72%) as a white solid.

Step C: 5- (Benzyloxy) -1H-indene
Stir a solution of the product from Step B (15.0 g, 62.5 mmol) in toluene (200 mL) to which p-TsOH (1.0 g, 6.25 mmol) is added at ambient temperature and the mixture is 1.5 hours at 80 ° C. Heated. The mixture was concentrated and purified by column chromatography (eluting with PE: EtOAc = 30: 1) to give the title compound (12.3 g, 89%) as a white solid.

Step D: ethyl (±) -exo-3- (benzyloxy) -1,1a, 6,6a-tetrahydrocyclopropa [a] indene-1-carboxylate
To a solution of the product from Step C (10 g, 45 mmol) and copper (I) trifluoromethanesulfonate (2: 1 complex with toluene, 0.23 g, 4.5 mmol) in dichloromethane (200 mL), dichloromethane (50 mL) In ethyl diazoacetate (47 mL, 450 mol) was added from a syringe pump at room temperature over 10 hours. The mixture was stirred at room temperature for a further 2 hours. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with PE = 100%) to give the title compound as a yellow oil (5.5 g, 40%).

Step E: ethyl (±) -exo-3-hydroxy-1,1a, 6,6a-tetrahydrocyclopropa [a] indene-1-carboxylate
To a mixture of Pd / C (0.4 g) in MeOH (20 mL) was added a solution of the product from Step D (4 g, 0.013 mol) in MeOH (20 mL) at room temperature. The mixture was stirred at room temperature for 2 hours under H ₂ atmosphere. The mixture was filtered and the filtrate was concentrated. The residue was purified by silica gel chromatography (eluting with EtOAc: PE = 1: 10) to give the title compound (2.3 g, 82%) as a colorless oil.

Step F: (±) -exo-3-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1,1a, 6,6a-tetrahydrocyclo Prop [a] indene-1-carboxylate
Product from Step E (2.0 g, 9 mmol), 5-fluoro-3,4-dihydro-1,8-naphthyridin-2 (1H) -one (1.5 g, 9 mmol) and cesium carbonate in DMF (30 mL) A mixture of (6 g, 18 mmol) was stirred at 120 ° C. for 2 hours. The reaction mixture was diluted with water (30 mL) and extracted with ethyl acetate (2 × 40 mL). The combined organic phases were washed with brine (50 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with EtOAc: PE = 1: 5 to 1: 1) to give the title compound (1.4 g, 42%) as a white solid.

Step G: (±) -exo-3-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1,1a, 6,6a-tetrahydrocyclo Propa [a] indene-1-carboxylic acid (Intermediate I)
Sodium hydroxide solution (7.7 mL, 2M, 15 mmol) was added to a stirred solution of the product from Step F (1.4 g, 3.8 mmol) in THF (24 mL) and methanol (24 mL) at room temperature. The mixture was stirred at 60 ° C. for 2 hours. The solvent was removed under reduced pressure and the residue was dissolved in water (20 mL). The solution was neutralized with HCl (1 mol / L) to pH = 7 and a white solid precipitated from the solution. The white solid was collected by filtration and air dried to give the title compound (0.9 g, 70%).

Stage H: (±) -exo-N- (2-aminophenyl) -3-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1 , 1a, 6,6a-Tetrahydrocyclopropa [a] indene-1-carboxamide
DIPEA (0.1 mL) was added to a solution of Intermediate I (30 mg, 0.089 mmol), benzene-1,2-diamine (9.6 mg, 0.089 mmol) and HATU (37 mg, 0.101 mmol) in DMF (1 mL) at room temperature. added. The mixture was stirred at room temperature overnight. The reaction mixture was diluted with water (5 mL) and extracted with ethyl acetate (2 × 10 mL). The combined organic phases were washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was used directly in the next step without further purification. MS: M / e 427 (M + 1) ⁺ .

Stage I: (±) -exo-5-((1- (1H-benzo [d] imidazol-2-yl) -1,1a, 6,6a-tetrahydrocyclopropa [a] inden-3-yl) oxy ) -3,4-Dihydro-1,8-naphthyridin-2 (1H) -one (compound 1.1)
A mixture of the product from Step H (38 mg, 0.089 mmol) in acetic acid (2 mL) was stirred at 80 ° C. for 3 hours. The solvent was removed and a solution of NaOH (2 mL, 2 mol / L) was added to the residue. The mixture was extracted with EtOAc (2 × 10 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound as a white solid.

Compound 1.2: (±) -exo-5-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1,1a, 6,6a-tetrahydrocyclopropa [a ] Inden-3-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one
To a solution of Intermediate I (338 mg, 1.0 mmol), 4- (trifluoromethyl) benzene-1,2-diamine (190 mg, 1.1 mmol) and DIPEA (500 mg, 3.9 mmol) in DMF (10 mL) was added HATU ( 405 mg, 1.1 mmol) was added at room temperature. The mixture was stirred at room temperature for 20 hours. The reaction mixture was diluted with ethyl EtOAc (50 mL). The mixture was washed with brine (3 × 20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure.

The residue was dissolved in acetic acid (10 mL) and the mixture was stirred at 90 ° C. for 5 hours. The solvent was removed. The residue was diluted with EtOAc (50 mL). The organic phase was washed with saturated sodium bicarbonate solution (2 × 20 mL), brine (2 × 20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with DCM: MeOH = 50: 1 to 20: 1) to give the title compound (245 mg, 51%) as a white solid.

Compound 1.2 was separated into two enantiomeric isomers (compound 1.2a, front peak, and compound 1.2b, back peak) by chiral preparative HPLC. The chiral separation conditions are shown below.

Compound 1.3

Step A: (±) -exo-N- (2-mercaptophenyl) -3-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1 , 1a, 6,6a-Tetrahydrocyclopropa [a] indene-1-carboxamide
To a solution of intermediate I (34 mg, 0.1 mmol) in CH ₂ Cl ₂ (3.0 mL) was added (COCl) ₂ (0.03 mL, 0.4 mmol) and DMF (catalytic amount) at room temperature under N ₂ . The mixture was stirred for 1.5 hours. The solvent was then removed and the residue was dissolved in CH ₂ Cl ₂ (3.0 mL), then DIPEA (0.07 mL, 0.4 mmol) and 2-aminobenzenethiol (14 mg, 0.11 mmol) were added at 0 ° C. under N _2. added. The mixture was stirred at room temperature for 2 hours. The mixture was diluted with CH ₂ Cl ₂ , washed with brine, dried over Na ₂ SO ₄ , filtered and concentrated. The residue was purified by preparative TLC (CH ₂ Cl ₂ : MeOH = 10: 1) to give the crude product (20 mg) as a yellow solid, which was used directly in the next step.

Step B: (±) -exo-5-((1- (benzo [d] thiazol-2-yl) -1,1a, 6,6a-tetrahydrocyclopropa [a] inden-3-yl) oxy)- 3,4-Dihydro-1,8-naphthyridin-2 (1H) -one (compound 1.3)
To a solution of the product from Step A (20 mg, crude) in toluene (4.0 mL) was added PPA (200 mg). The mixture was stirred at 140 ° C. for 3 hours. The reaction mixture was cooled to room temperature and the solvent was removed. The residue was dissolved with CH ₂ Cl ₂ (10 mL), washed with saturated NaHCO ₃ solution and extracted with CH ₂ Cl ₂ (2 × 15 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (3.8 mg, 9%, 2 steps) as a pale yellow solid.

Example 2: Synthesis of compounds 2.1-2.37 Compound 2.1

Stage A: (Benzofuran-5-yloxy) (tert-butyl) dimethylsilane
To a stirred solution of benzofuran-5-ol (5.2 g, 0.039 mol) in DMF (50 mL) was added imidazole (5.3 g, 0.078 mol) and TBSCl (6.1 g, 0.041 mol) at room temperature. The mixture was stirred at room temperature for 20 hours. Saturated NaHCO ₃ solution (150 mL) was then added to the reaction mixture and the mixture was extracted with EtOAc (3 × 100 mL). The combined organic phases were washed with brine (3 × 100 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with 100% PE) to give the title compound (7.8 g, 81%) as a colorless oil.

Step B: (±) -Exo-5-((tert-butyldimethylsilyl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate
To a solution of the product from Step A (9.25 g, 0.037 mol) and copper (I) trifluoromethanesulfonate (2: 1 complex with toluene, 1.9 g, 3.7 mmol) in dichloromethane (200 mL) was added dichloromethane ( 50 mL) was added ethyl diazoacetate (38.7 mL, 0.37 mol) from a syringe pump over 10 hours. The mixture was then stirred at room temperature for a further 2 hours. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with PE = 100%) to give the title compound. The product was used in the next step without further purification.

Step C: (±) -Exo-5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate
To a solution of the product from Step B (10 g, 0.030 mol) in THF (25 mL), TBAF (15 mL, 1 M, 0.015 mol) in THF was added dropwise at 0 ° C. The mixture was then stirred at room temperature for 1 hour. The reaction mixture was diluted with water (30 mL) and extracted with ethyl acetate (2 × 50 mL). The combined organic phases were washed with brine (2 × 50 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with EtOAc: PE = 1: 10) to give the title compound (3.8 g, 46% yield over 2 steps) as a colorless oil.

Step D: (±) -exo-5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cycloprop b] Ethyl benzofuran-1-carboxylate
Product from Step C (3.8 g, 0.017 mol), 5-fluoro-3,4-dihydro-1,8-naphthyridin-2 (1H) -one (2.85 g, 0.017 mol) in DMF (50 mL) and A mixture of cesium carbonate (11.2 g, 0.034 mol) was stirred at 120 ° C. for 2 hours. The reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (2 × 30 mL). The combined organic phases were washed with brine (50 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with EtOAc: PE = 1: 5 to 1: 1) to give the title compound (2.8 g, 44%) as a white solid.

Step E: (±) -exo-5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cycloprop b] Benzofuran-1-carboxylic acid (Intermediate II)
Aqueous sodium hydroxide (18 mL, 2M, 36 mmol) was added to a stirred solution of the product from Step D (2.8 g, 7.7 mmol) in THF (54 mL) and methanol (54 mL) at room temperature. The mixture was stirred at 60 ° C. for 2 hours. The solvent was removed under reduced pressure and the residue was dissolved in water (20 mL). The solution was neutralized with HCl (1 mol / L) to pH = 7 and a white solid precipitated from the solution. The white solid was collected by filtration and air dried to give the title compound (2.4 g, 92.7%).

Step F: (±) -Exo-N- (2-aminophenyl) -5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a , 6b-Dihydro-1H-cyclopropa [b] benzofuran-1-carboxamide
Product from Step E (30 mg, 0.088 mmol), benzene-1,2-diamine (9.6 mg, 0.088 mmol), DIPEA (17.2 mg, 0.130 mmol) and HATU (37 mg, 0.101 mmol) in DMF (1 mL) The mixture was stirred at room temperature overnight. The reaction mixture was diluted with water (5 mL) and extracted with ethyl acetate (2 × 10 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

Stage G: (±) -exo-5-((1- (1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy)- 3,4-Dihydro-1,8-naphthyridin-2 (1H) -one (Compound 2.1)
A mixture of the product from Step F (37 mg, 0.088 mmol) in acetic acid (1 mL) was stirred at 80 ° C. for 3 hours. The solvent was removed and a solution of NaOH (2 mL, 2 mol / L) was added to the residue. The mixture was extracted with EtOAc (2 × 10 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (10 mg, 41%) as a white solid.

Stereoselective synthesis of ethyl (1S, 1aS, 6bR) -5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylate (intermediate III)

Step A: (1S, 1aS, 6bR) -5-((tert-butylmethylsilyl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate copper trifluoromethanesulfonate (I ) (2: 1 complex with toluene, 250 mg, 2.4%) and (S, S)-(+)-2,2-isopropylidenebis (4-tert-butyl) -2-oxazoline (205 mg, 3.4% ) In dichloromethane (15 mL) under N ₂ atmosphere at ambient temperature for 1 hour. A solution of (benzofuran-5-yloxy) (tert-butyl) dimethylsilane (5.0 g, 20.2 mmol) in dichloromethane (85 mL) was added, followed by ethyl diazoethanoate (40 mL, 380 mmol) using a syringe pump for 10 hours. Slowly added between. The mixture was concentrated and purified by column chromatography to give the crude compound (7.3 g), which was used in the next step without further purification.

Step B: (1S, 1aS, 6bR) -5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate (Intermediate III)
Tetra-n-butylammonium fluoride (TBAF, 1M in THF, 4 mmol) was added dropwise at 0 ° C. to a solution of the product of Step A (7.3 g, crude) in THF (100 mL). The reaction mixture was stirred at room temperature for 30 minutes. The mixture was concentrated and purified by column chromatography to give the title compound as a solid (2.52 g, 57% in two steps, 72% ee).

Compound 2.2: (±) -exo-5-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran -5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one
Intermediate II (1 g, 2.96 mmol), 4- (trifluoromethyl) benzene-1,2-diamine (0.52 g, 2.96 mmol), DIPEA (1.15 g, 8.88 mmol) and HATU (1.69) in DMF (5 mL) g, 4.44 mmol) was stirred at room temperature overnight. The reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (2 × 30 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure.

The residue was dissolved in acetic acid (10 mL). The mixture was stirred at 80 ° C. for 3 hours. The solvent was removed and a solution of NaOH (20 mL, 2 mol / L) was added to the residue. The mixture was extracted with EtOAc (2 × 30 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (330 mg, 23.6%) as a white solid.

Compound 2.2 was separated into two enantiomeric stereoisomers (compound 2.2a, front peak, and compound 2.2b, back peak) by chiral preparative HPLC. The chiral separation conditions are shown below:

Compound 2.2b: 5-(((1R, 1aS, 6bR) -1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [ b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one

Step A: (1S, 1aS, 6bR) -5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro-1H- Cyclopropa [b] ethyl benzofuran-1-carboxylate
Intermediate III (400 mg, 1.8 mmol), 5-fluoro-3,4-dihydro-1,8-naphthyridin-2 (1H) -one (250 mg, 1.5 mmol) and cesium carbonate (801 mg, DMF (20 mL) 2.3 mol) was stirred at 120 ° C. for 2 hours. The reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (2 × 10 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with EtOAc: PE = 3: 1) to give the title compound (360 mg, 54.6%) as a white solid.

Step B: (1S, 1aS, 6bR) -5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro-1H- Cyclopropa [b] benzofuran-1-carboxylic acid
A solution of the ester product of Step A (360 mg, 0.98 mmol) in THF (3 mL) and methanol (3 mL) was stirred and to this was added aqueous sodium hydroxide (1 mL, 2M, 2 mmol) at room temperature. The mixture was stirred at 60 ° C. for 2 hours. The solvent was removed under reduced pressure and the residue was dissolved in water (5 mL). The solution was neutralized with HCl (2 mol / L) to pH = 7 and a white solid precipitated from the solution. The white solid was collected by filtration and air dried to give the title compound (230 mg, 69.3%).

Step C: 5-(((1R, 1aS, 6bR) -1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b ] Benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one
Stage B product (50 mg, 0.15 mmol), 4- (trifluoromethyl) benzene-1,2-diamine (26 mg, 0.15 mmol), DIPEA (0.1 mL) and HATU (84 mg, 0.22) in DMF (1 mL) mmol) was stirred at room temperature overnight. The reaction mixture was diluted with water (5 mL) and extracted with ethyl acetate (2 × 10 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure.

  The residue was dissolved in acetic acid (1 mL). The mixture was stirred at 80 ° C. for 3 hours. The solvent was removed and a solution of NaOH (2 mL, 2 mol / L) was added to the residue. The mixture was extracted with EtOAc (2 × 5 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (10 mg, 14.1% yield; 69.6% ee) as a white solid.

Compound 2.3: (±) -exo-5-((1- (5,6-dichloro-1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5 -Yl) oxy) -3,4-dihydro-1,8-naphthyridine-2 (1H) -one
Intermediate II (600 mg, 1.8 mmol), 4,5-dichlorobenzene-1,2-diamine (312 mg, 1.8 mmol), DIPEA (0.69 g, 5.4 mmol) and HATU (1.01 g, 2.7) in DMF (10 mL) mmol) was stirred at room temperature overnight under a nitrogen atmosphere. The reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (2 × 30 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure.

The residue was dissolved in acetic acid (10 mL). The mixture was stirred at 80 ° C. for 3 hours. The solvent was removed and a solution of NaOH (10 mL, 2 mol / L) was added to the residue. The mixture was extracted with EtOAc (2 × 30 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (320 mg, 37.6%) as a white solid.

Compound 2.3 was separated into two enantiomeric stereoisomers (compound 2.3a, front peak, and compound 2.3b, back peak) by chiral preparative HPLC. The chiral separation conditions are shown below:

Compound 2.4: (±) -exo-5-((1- (5-fluoro-6-methyl-1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran -5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one
To a solution of Intermediate II (338 mg, 1.0 mmol), 4-fluoro-5-methylbenzene-1,2-diamine (169 mg, 1.2 mmol), and triethylamine (300 mg, 3.0 mmol) in DMF (7 mL) was added HATU ( 420 mg, 1.1 mmol) was added at room temperature. The mixture was stirred at room temperature for 16 hours. The reaction mixture was diluted with water (30 mL) and a white solid precipitated. The mixture was filtered and the solid was thoroughly dried under reduced pressure.

The solid was dissolved in acetic acid (7 mL) and the mixture was stirred at 85 ° C. for 8 hours. The solvent was removed. The residue was diluted with DCM (20 mL) and the organic phase was washed with a solution of NaOH (2 mol / L, 5 mL). The aqueous phase was further extracted with 50 mL DCM. The combined organic phases were washed with brine (2 × 20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with DCM: MeOH = 50: 1 to 20: 1) to give the title compound (230 mg, 52%) as a brown solid.

Compound 2.4 was separated into two enantiomeric stereoisomers (compound 2.4a, front peak, and compound 2.4b, back peak) by chiral preparative HPLC. The chiral separation conditions are shown below:

Compound 2.5: (±) -exo-5-((1- (5-chloro-6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one
To a solution of intermediate II (300 mg, 0.887 mmol) and triethylamine (500 mg, 5 mmol) in DMF (5 mL) was added HATU (370 mg, 0.97 mmol) at 0 ° C. After the mixture was stirred for 15 minutes, 4-chloro-5- (trifluoromethyl) benzene-1,2-diamine (228 mg, 1.09 mmol) in DMF (1 mL) was added to the reaction mixture. The mixture was allowed to warm to room temperature and stirred for 20 hours. The reaction mixture was diluted with water (50 mL) and a white solid precipitated. The mixture was filtered and the solid was thoroughly dried under reduced pressure.

The solid was dissolved in acetic acid (5 mL) and the mixture was stirred at 85 ° C. for 5 h. The solvent was removed. The residue was purified by silica gel chromatography (eluting with DCM: MeOH = 50: 1 to 20: 1) to give the title compound (205 mg, 45%) as a pale yellow solid.

Compound 2.5 was separated into two enantiomeric isomers (compound 2.5a, front peak, and compound 2.5b, back peak) by chiral preparative HPLC. The chiral separation conditions are shown below:

  Compounds 2.6-2.25 were prepared according to the procedure described for compound 2.1 using the corresponding benzofuran and diamine as starting materials under appropriate conditions that would be understood by one skilled in the art.

Compound 2.6

Compound 2.7

Compound 2.8

Compound 2.9

Compound 2.10

Compound 2.11

Compound 2.12

Compound 2.13

Compound 2.14

Compound 2.15

Compound 2.16

Compound 2.17

Compound 2.18

Compound 2.19

Compound 2.20

Compound 2.21

Compound 2.22

Compound 2.23

Compound 2.24

Compound 2.25

Compound 2.26: (±) -exo-5-((1- (7-chloro-1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl ) Oxy) -3,4-dihydro-1,8-naphthyridine-2 (1H) -one
To a solution of intermediate II (30 mg, 0.09 mmol) and 3-chloro-2-nitrobenzenamine (15.3 mg, 0.09 mmol) in pyridine (1 mL) was added phosphoryl trichloride (2 drops) at 0 ° C. The mixture was then stirred at room temperature for 30 minutes. The reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (2 × 10 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was dissolved in acetic acid (2 mL) and iron powder (15 mg, 0.27 mmol) was added to the reaction mixture. The mixture was stirred at 70 ° C. for 2 hours. The reaction mixture was diluted with methanol (20 ml) and filtered through a celite pad. The filtrate was concentrated under reduced pressure and the residue was purified by preparative HPLC to give the title compound (6 mg, 15%) as a white solid.

Compound 2.27: (±) -exo-5-((1- (6-hydroxy-1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl ) Oxy) -3,4-dihydro-1,8-naphthyridine-2 (1H) -one

Stage A: 4-((tert-Butyldimethylsilyl) oxy) -2-nitroaniline
To a solution of 4-amino-3-nitrophenol (1.0 g, 6.5 mmol) and TBSCl (1.32 g, 8.8 mmol) in DMF (10 mL) was added imidazole (0.88 g, 13.0 mmol) at room temperature. The mixture was stirred at room temperature overnight. The mixture was diluted with ethyl acetate (300 mL), washed with brine (100 mL), dried over anhydrous sodium sulfate and concentrated. The residue was purified by silica gel chromatography (eluting with PE: EtOAc 2: 1) to give the title compound (1.85 g, 100%) as a gray solid.

Step B: 4-((tert-Butyldimethylsilyl) oxy) benzene-1,2-diamine
A mixture of 4- (tert-butyldimethylsilyloxy) -2-nitrobenzenamine (0.5 g, 1.9 mmol), Fe powder (1.1 g, 19 mmol) and acetic acid (1.0 mL, 18 mmol) in EtOH (10 mL) was refluxed. For 4 hours. The mixture was filtered through a celite pad and the filtrate was concentrated. The residue was diluted with ethyl acetate (100 mL), washed with saturated NaHCO ₃ solution (3 × 30 mL) and brine (30 mL), dried over anhydrous sodium sulfate and concentrated. The residue was purified by flash silica gel chromatography (eluting with PE: EtOAc 2: 1) to give the title compound (0.34 g, 77%) as a gray liquid.

Step C: (±) -exo-5-((1- (6-((tert-butyldimethylsilyl) oxy) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H- Cyclopropa [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one
Intermediate II (30 mg, 0.09 mmol), 4- (tert-butyldimethylsilyloxy) benzene-1,2-diamine (9.6 mg, 0.089 mmol), DIPEA (0.5 mL) and HATU (37 mg) in DMF (2 mL) , 0.101 mmol) was stirred at room temperature overnight. The reaction mixture was diluted with ethyl acetate (20 mL), washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

  A mixture of the above products in acetic acid (2 mL) was stirred at 80 ° C. for 3 hours. The solvent was removed and the residue was diluted with water and adjusted to pH 7-8 with 2N NaOH. The mixture was extracted with DCM (2 × 10 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound as a white solid (20 mg, 40%).

Stage D: (±) -exo-5-((1- (6-hydroxy-1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl ) Oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one (compound 2.27)
To a solution of the above product (30 mg, 0.037 mmol) in THF (2 mL), TBAF in THF (0.01 mL, 1 M, 0.01 mmol) was added dropwise at 0 ° C. The mixture was then stirred at room temperature for 30 minutes. The reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (20 mL × 2). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (10 mg, 63%) as a white solid.

Compound 2.28: (±) -exo-5-((1- (6-phenyl-1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl ) Oxy) -3,4-dihydro-1,8-naphthyridine-2 (1H) -one
5-((1- (6-Bromo-1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] in a mixed solution of dioxane and water (2 mL, 4/1) ] Benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one (22 mg, 0.045 mmol), phenylboronic acid (16.5 mg, 0.135 mmol), Pd (PPh ₃ A mixture of) ₄ (20 mg, 0.017 mmol) and K ₂ CO ₃ (21 mg, 0.15 mmol) was stirred at reflux temperature for 5 h under N ₂ . The mixture was diluted with brine (2 mL) and extracted with ethyl acetate (2 mL × 3). The combined organics were washed with brine (2 mL × 3), dried over Na ₂ SO ₄ and concentrated. The residue was purified by preparative HPLC to give the title compound (12 mg, 55%) as a white solid.

Compound 2.29: (±) -exo-5-((1- (3-phenyl-1,2,4-oxadiazol-5-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5 -Yl) oxy) -3,4-dihydro-1,8-naphthyridine-2 (1H) -one

Stage A: (±) -exo-N-((hydroxyimino) (phenyl) methyl) -5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) Oxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxamide
A mixture of Intermediate II (30 mg, 0.088 mmol), N-hydroxybenzimidoamide (12 mg, 0.088 mol), HATU (37 mg, 0.1 mmol) and DIEPA (17 mg, 0.13 mmol) in DMF (1 mL) at room temperature. Stir overnight. The reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (2 × 15 mL). The combined organic phases were washed with brine (2 × 5 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue (50 mg) was used directly in the next step without further purification.

Step B: (±) -exo-5-((1- (3-phenyl-1,2,4-oxadiazol-5-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5 -Yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one (compound 2.29)
A mixture of the product from Step A (50 mg, crude) in pyridine (1 mL) was stirred at 100 ° C. for 2 hours. The reaction mixture was cooled to room temperature and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (10 mg, 26%) as a white solid.

Compound 2.30: (±) -exo-5-((1- (4-phenyl-1H-imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy)- 3,4-Dihydro-1,8-naphthyridin-2 (1H) -one

Step A: (±) -exo-5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cycloprop b] 2-oxo-2-phenylethyl benzofuran-1-carboxylate
A mixture of Intermediate II (30 mg, 0.088 mmol), 2-bromo-1-phenylethanone (35 mg, 0.176 mol) and K ₂ CO ₃ (36 mg, 0.264 mmol) in DMF (1 mL) was stirred at room temperature for 0.5 h. did. The reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (2 × 15 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue (50 mg) was used directly in the next step without further purification.

Step B: (±) -exo-5-((1- (4-phenyl-1H-imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy)- 3,4-Dihydro-1,8-naphthyridin-2 (1H) -one (compound 2.30)
A mixture of the product from Step A (50 mg, crude) and NH ₄ OAc in AcOH (1 mL) was stirred at 120 ° C. for 10 hours. The reaction mixture was cooled to room temperature and the mixture was diluted with saturated NaHCO ₃ solution (30 mL) and extracted with ethyl acetate (2 × 15 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (7 mg, 18%) as a white solid.

Compound 2.31
Compound 2.31 was prepared using 2-bromo-1- (pyridin-4-yl) ethanone following the procedure described for compound 2.30.

Compound 2.32
Compound 2.32 was prepared using 2-bromo-1- (2-chlorophenyl) ethanone following the procedure described for compound 2.30.

Compound 2.33: (±) -exo-5-((1- (4- (trifluoromethyl) -1H-imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl ) Oxy) -3,4-dihydro-1,8-naphthyridine-2 (1H) -one

Stage A: (±) -exo-5-((1- (hydroxymethyl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8 -Naphthyridine-2 (1H) -one
5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran in THF (10 mL) To a mixture of ethyl-1-carboxylate (Intermediate II, 217 mg, 0.59 mmol) was added LiAlH ₄ (27 mg, 0.71 mmol). The reaction mixture was stirred at room temperature for 0.25 hours. Water (10 mL) was added dropwise and the mixture was extracted with ethyl acetate (2 × 15 mL). The combined organic layers were washed with brine (10 mL), dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue (190 mg) was used directly in the next step without further purification.

Step B: (±) -exo-5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cycloprop b] benzofuran-1-carbaldehyde
A mixture of Step A product (190 mg, crude) and PCC (255 mg, 1.2 mmol) in DCM (5 mL) was stirred at room temperature overnight. The mixture was diluted with saturated water (30 mL) and extracted with DCM (2 × 15 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative TLC (DCM / MeOH = 15/1) to give the title compound (35 mg, 17% over 2 steps) as a white solid.

Step C: (±) -exo-5-((1- (4- (trifluoromethyl) -1H-imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl ) Oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one (compound 2.33)
3,3-Dibromo-1,1,1-trifluoropropan-2-one (30 mg, 0.11 mmol) and NaOAc (8 mg, 0.11 mmol) were dissolved in water (1 mL) and heated at 100 ° C. for 1 hour. The mixture was added to a solution of the product of Step B (35 mg, 0.1 mmol) and ammonium hydroxide (0.5 mL) in methanol (3 mL). The resulting mixture was stirred at room temperature for 40 minutes and then heated to reflux for 1 hour. After cooling, the mixture was concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (8 mg, 19%) as a white solid.

Compound 2.34: (±) -exo-5-((1- (1- (2-hydroxyethyl) -5- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b- Dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one

Stage A: 2-((2-Nitro-4- (trifluoromethyl) phenyl) amino) ethanol
To a solution of 2-aminoethanol (1.76 g, 28.8 mmol) in THF (10 mL) was added 1-fluoro-2-nitro-4- (trifluoromethyl) benzene (2.0 g, 9.6 mmol) at 0 ° C. . The solution was returned to room temperature and stirred at room temperature for 2 hours. The solvent was removed under reduced pressure and the residue was diluted with EtOAc (100 mL), washed with brine (30 mL × 3), dried over anhydrous sodium sulfate, filtered and concentrated to give the title compound (2.4 g, 100 %) Which was used in the next step without further purification.

Step B: N- (2-((tert-butyldimethylsilyl) oxy) ethyl) -2-nitro-4- (trifluoromethyl) aniline
To a solution of the product of Step A (2.4 g, 9.6 mmol) and TBSCl (1.73 g, 11.5 mmol) in DMF (15 mL) was added imidazole (1.3 g, 19.2 mmol) at room temperature. The mixture was stirred at room temperature overnight. The mixture was diluted with ethyl acetate (100 mL), washed with brine (30 mL × 3), dried over anhydrous sodium sulfate and concentrated. The residue was purified by silica gel chromatography (eluting with PE) to give the title compound (3.5 g, 100%) as a yellow solid.

Step C: N1- (2-((tert-butyldimethylsilyl) oxy) ethyl) -4- (trifluoromethyl) benzene-1,2-diamine
A mixture of Step B product (3.5 g, 9.6 mmol) and Pd / C (0.3 g) in MeOH (20 mL) was stirred at room temperature for 4 h under H ₂ atmosphere. The mixture was filtered through a celite pad and the filtrate was concentrated. The residue was then diluted with ethyl acetate (100 mL), washed with brine (30 mL × 3), dried over anhydrous sodium sulfate and concentrated to give the title compound (3.1 g, 90%) as a purple solid. .

Step D: (±) -exo-5-((1- (1- (2-hydroxyethyl) -5- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b- Dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one (compound 2.34)
A mixture of Intermediate II (300 mg, 0.9 mmol), Step C product (440 mg, 1.45 mmol), DIPEA (1 mL), and HATU (551 mg, 1.45 mmol) in DMF (5 mL) was stirred at room temperature overnight. The reaction mixture was diluted with ethyl acetate (20 mL), washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

A mixture of the crude product in acetic acid (5 mL) was stirred at 80 ° C. for 8 hours. The solvent was removed and the residue was diluted with water. The pH value of the resulting mixture was adjusted to 7-8 with 2N NaOH. The mixture was extracted with DCM (2 × 10 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by flash silica gel chromatography (eluting with PE: EtOAc 1: 3) to give the crude product, which was then further purified by preparative HPLC to give the title compound as a white solid (61 mg, 13% ).

Compound 2.35: (±) -exo-5-((3-methyl-1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one

Stage A: 2-bromo-4-methoxyphenol
To a solution of 4-methoxyphenol (10 g, 81 mmol) in CHCl ₃ (50 mL), BR ² (4 mL, 78 mmol) was added at 0 ° C. The mixture was stirred at 0 ° C. for 1 hour, then allowed to warm to room temperature and the mixture was stirred at room temperature for 1 hour. The resulting mixture was diluted with dichloromethane (500 mL), washed with saturated NaHSO3 (3 × 100 mL) and brine (200 mL), dried over anhydrous sodium sulfate, filtered and concentrated to give the title compound (16.6 g, 100%) was obtained as an off-white solid, which was used in the next step.

Step B: 2-Bromo-1- (2,2-diethoxyethoxy) -4-methoxybenzene
Mixture of product from Step A (1.01 g, 5 mmol), 2-bromo-1,1-diethoxyethane (1.05 g, 5.25 mmol) and Cs ₂ CO ₃ (2.04 g, 6 mmol) in DMF (10 mL) Was stirred at 120 ° C. for 2 hours. The reaction mixture was cooled to room temperature. The mixture was diluted with EtOAc (200 mL), washed with brine (50 mL), dried over anhydrous sodium sulfate and concentrated. The residue was purified by column chromatography (eluting with PE: EtOAc = 6: 1) to give the title compound (2.7 g, 85%) as an oil.

Stage C: 7-Bromo-5-methoxybenzofuran
To a mixture of the product from Step B (2.7 g, 8.5 mmol) in toluene (10 mL) was added PPA (1.0 mL). The reaction mixture was heated at 70 ° C. for 1 hour. The reaction mixture was cooled to room temperature. The mixture was diluted with EtOAc (200 mL) and the aqueous phase was adjusted to pH 7-8 with NaOH (2 mol / L). The organic phase was washed with brine (50 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica column chromatography (eluting with PE) to give the title compound (0.27 g, 16%) as an oil.

Stage D: 5-methoxy-7-methylbenzofuran
Product from Step C (3.6 g, 16 mmol), methylboronic acid (1.4 g, 24 mmol), Pd (dppf) ₂ Cl ₂ (0.65 g, in 1,4-dioxane (50 mL) and H ₂ O (10 mL) 0.8 mmol) and Cs ₂ CO ₃ (13.0 g, 40 mmol) were refluxed for 3 h under N ₂ atmosphere. The reaction mixture was cooled to room temperature and filtered through a celite pad. The filtrate was diluted with EtOAc (200 mL), washed with brine (50 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica column chromatography (eluting with PE) to give the title compound (1.0 g, 38%) as an oil.

Stage E: 7-Methylbenzofuran-5-ol
To a mixture of the product from Step D (850 mg, 5.2 mmol) and K ₂ CO ₃ in acetonitrile (10 mL) was added TMSI (1.1 mL). The mixture was refluxed for 2 hours. The reaction mixture was cooled to room temperature. The mixture was diluted with EtOAc (100 mL), washed with brine (30 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting with PE: EtOAc = 5: 1) to give the title compound (200 mg, 25%) as an oil.

Step F: tert-Butyldimethyl ((7-methylbenzofuran-5-yl) oxy) silane
A solution of the product from Step E (200 mg, 1.4 mmol), TBSCl (225 mg, 1.5 mmol) and imidazole (190 mg, 2.8 mmol) in DMF (5 mL) was stirred at room temperature for 1 hour. The mixture was diluted with EtOAc (100 mL), washed with brine (30 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluting with PE) to give the title compound as an oil (260 mg, 71%).

Step G: (±) -Exo-5-((tert-butyldimethylsilyl) oxy) -3-methyl-1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate
To a solution of the product from Step F (260 mg, 1 mmol) and copper (I) trifluoromethanesulfonate (2: 1 complex with toluene, 1 mg, 0.03 mmol) in dichloromethane (2 mL) in dichloromethane (10 mL) Of diazoacetate (1.0 mL, 10 mol) was added from a syringe pump over 10 hours. The solvent was removed under reduced pressure and the residue was purified by silica gel chromatography (eluting with PE) to give the title compound (200 mg, crude), which was used in the next step without further purification.

Stage H: (±) -Exo-5-hydroxy-3-methyl-1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate
To a solution of the product from Step G (200 mg, 0.6 mmol) in THF (5 mL), TBAF in THF (0.15 mL, 1M, 0.15 mmol) was added dropwise at 0 ° C. The mixture was then stirred at room temperature for 10 minutes. The reaction mixture was concentrated and purified by silica gel chromatography (eluting with EtOAc: PE = 1: 10) to give the title compound (100 mg, 43% yield over 2 steps) as a colorless oil, which in the next step Used directly.

Step I: (±) -exo-3-methyl-5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro- 1H-Cyclopropa [b] ethyl benzofuran-1-carboxylate
Product from Stage H (90 mg, 0.38 mmol), 5-fluoro-3,4-dihydro-1,8-naphthyridin-2 (1H) -one (64 mg, 0.38 mmol) and cesium carbonate in DMF (5 mL) A mixture of (188 mg, 0.58 mmol) was stirred at 120 ° C. for 2 hours. The reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (2 × 20 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was used in the next step without further purification.

Step J: (±) -exo-3-methyl-5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro- 1H-cyclopropa [b] benzofuran-1-carboxylic acid
Sodium hydroxide solution (3 mL, 2M) was added to a stirred solution of the ester product from Step I (60 mg, 3.8 mmol) in methanol (9 mL) at room temperature. The mixture was stirred at room temperature overnight. The solvent was removed under reduced pressure and the residue was dissolved in water (10 mL). The solution was neutralized with HCl (2 mol / L) to pH = 7 and extracted with EA (2 × 10 mL). The combined organic phases were washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was used in the next step without further purification.

Step K: (±) -exo-5-((3-methyl-1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cycloprop [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one (compound 2.35)
Product from Step J (45 mg, 0.13 mmol), 4- (trifluoromethyl) benzene-1,2-diamine (25 mg, 0.14 mmol), DIPEA (0.5 ml) and HATU (61 mg, in DMF (2 mL) 0.16 mmol) was stirred at room temperature for 2 hours. Water (2 mL) was added and the precipitated solid was collected and washed with water (2 mL) to give the intermediate amide product.

A solution of the intermediate amide product in acetic acid (2 mL) was stirred at 80 ° C. for 2 hours. The solvent was removed and an aqueous solution of NaOH (2 mL, 2 mol / L) was added to the residue. The mixture was extracted with EtOAc (2 × 10 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (6 mg, 9.5%) as a white solid.

Compound 2.36: (±) -exo-5-((4-chloro-1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one

Step A: (±) -Exo-4-chloro-5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate
To a solution of ethyl 5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylate (product from Step C in the synthesis of Compound 2.1, 500 mg, 2.3 mmol) in acetonitrile (10 mL) , NCS (302 mg, 2.3 mmol) was added at room temperature. The mixture was stirred at room temperature overnight. The reaction mixture was diluted with water (20 mL) and extracted with EtOAc (2 × 10 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was used in the next step without further purification.

Stage B: (±) -exo-4-chloro-5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro- 1H-Cyclopropa [b] ethyl benzofuran-1-carboxylate
Product A from Stage A (577 mg, 2.3 mmol), 5-fluoro-3,4-dihydro-1,8-naphthyridin-2 (1H) -one (377 mg, 2.3 mmol) and carbonic acid in DMF (20 mL) A mixture of cesium (1.1 g, 3.4 mmol) was stirred at 120 ° C. for 2 hours. The reaction mixture was diluted with water (40 mL) and extracted with ethyl acetate (2 × 30 mL). The combined organic phases were washed with brine (40 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with EtOAc: PE = 1: 5 to 1: 1) to give the title compound (320 mg, 80%) as a white solid.

Step C: (±) -exo-4-chloro-5-((7-oxo-5,6,7,8-tetrahydro-1,8-naphthyridin-4-yl) oxy) -1a, 6b-dihydro- 1H-cyclopropa [b] benzofuran-1-carboxylic acid
Sodium hydroxide solution (1 mL, 2M) was added to a stirred solution of the ester product from Step B (320 mg, 0.8 mmol) in methanol (3 mL) at room temperature. The mixture was stirred at room temperature for 2 hours. The solvent was removed under reduced pressure and the residue was dissolved in water (10 mL). The solution was neutralized with HCl (1 mol / L) to pH = 7 and a white solid precipitated from the solution. The white solid was collected by filtration and air dried to give the title compound (230 mg, 77.2%).

Step D: (±) -exo-5-((4-chloro-1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cycloprop [b] Benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one (compound 2.36)
To a solution of the product from Step C (60 mg, 0.16 mmol), 4-fluoro-5-methylbenzene-1,2-diamine (22.6 mg, 0.16 mmol), and DIPEA (0.1 mL) in DMF (2 mL) , HATU (92 mg, 0.24 mmol) was added at room temperature. The mixture was stirred at room temperature for 20 hours. The reaction mixture was extracted with EtOAc (5 mL). The mixture was washed with brine (3 × 5 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure.

The residue was dissolved in acetic acid (5 mL) and the mixture was stirred at 80 ° C. for 3 hours. The solvent was removed and a solution of NaOH (2 mol / L, 10 mL) was added to the residue. The aqueous phase was extracted with EtOAc (2 × 20 mL). The combined organic phases were washed with brine (20 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (20 mg, 26.0%) as a white solid.

Compound 2.37: (±) -exo-5-((4-chloro-1- (6-fluoro-5-methyl-1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cycloprop [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one
Compound 2.37 is obtained for compound 2.36 by using 4-fluoro-5-methylbenzene-1,2-diamine from the product of Step C in the synthesis of compound 2.36 under appropriate conditions that can be understood by one skilled in the art. Prepared according to the procedure described.

Example 3: Synthesis of compounds 3.1 to 3.8 Compound 3.1: (±) -exo -N-methyl-4-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazole- 2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy) picolinamide

Step A: (±) -Exo-5-((2- (methylcarbamoyl) pyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate
Ethyl 5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylate (compound from step C in compound 2.1, 50 mg, 0.23 mmol), 4-chloro-N in DMF (4 mL) A mixture of methylpicolinamide (39 mg, 0.23 mol) and cesium carbonate (225 mg, 0.69 mmol) was stirred at 120 ° C. for 30 minutes. The reaction mixture was diluted with water (10 mL) and extracted with ethyl acetate (3 × 30 mL). The combined organic phases were washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 2) to give the title compound (40 mg, 50%) as a white solid.

Step B: (±) -Exo-5-((2- (methylcarbamoyl) pyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylic acid
A mixture of the product from Step A (60 mg, 0.17 mmol), aqueous sodium hydroxide (0.34 mL, 2M, 0.68 mmol), THF (2 mL) and methanol (2 mL) was stirred at 60 ° C. for 1 hour. The solution was neutralized with HCl (2 mol / L) to pH = 7 and the solvent was removed. The residue was dissolved in ethyl acetate (3 × 30 mL), washed with water (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (57 mg, 100%). This was used directly in the next step. MS: M / e 327 (M + 1) ⁺ .

Step C: (±) -exo-N-methyl-4-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] Benzofuran-5-yl) oxy) picolinamide (Compound 3.1)
Product B from Step B in DMF (3 mL) (57 mg, 0.17 mmol), 4- (trifluoromethyl) benzene-1,2-diamine (34 mg, 0.19 mmol), DIPEA (0.035 mL, 0.20 mmol) and A mixture of HATU (72 mg, 0.19 mmol) was stirred at room temperature overnight. The reaction mixture was extracted with ethyl acetate (3 × 20 mL), washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

The crude product was dissolved in acetic acid (3 mL) and stirred at 60 ° C. for 6 hours. The mixture was extracted with EtOAc (50 mL), basified with aqueous NaHCO ₃ solution, washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 2) to give the title compound (50 mg, 63%) as a white solid.

Compound 3.2: (±) -exo-2- (5- (pyrimidin-4-yloxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-yl) -6- (trifluoromethyl) -1H -Benzo [d] imidazole

Step A: (±) -Exo-5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylic acid
Ethyl 5-((tert-butyldimethylsilyl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylate in methanol (13.5 mL) (formation from step B in the synthesis of compound 2.1 Product, 1.5 g, 4.5 mmol) was stirred and aqueous sodium hydroxide (4.5 mL, 2M, 9.0 mmol) was added to this at room temperature. The mixture was stirred at 60 ° C. for 2 hours. The solvent was removed under reduced pressure and the residue was dissolved in water (30 mL). The solution was neutralized with HCl (2 mol / L) to pH = 7 and a white solid precipitated from the solution. The white solid was collected by filtration and air dried to give the title compound (450 mg, 52%), which was used directly in the next step.

Step B: (±) -exo-1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-ol
Product from Step A (100 mg, 0.52 mmol), 4- (trifluoromethyl) benzene-1,2-diamine (101 mg, 0.57 mmol), DIPEA (0.11 mL, 0.62 mmol) and HATU in DMF (5 mL) A mixture of (217 mg, 0.57 mmol) was stirred at room temperature overnight. The reaction mixture was CH ₂ Cl ₂ (3 × 30 mL), washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

The crude product was dissolved in acetic acid (3 mL) and stirred at 60 ° C. for 1.5 hours. The mixture was basified with aqueous NaHCO ₃ , extracted with EtOAc (3 × 30 mL), washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 2) to give the title compound (30 mg, 17%) as a brown oil.

Step C: (±) -exo-2- (5- (pyrimidin-4-yloxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-yl) -6- (trifluoromethyl) -1H -Benzo [d] imidazole (compound 3.2)
A mixture of the product from Step B (20 mg, 0.06 mmol), 4-chloropyrimidine (8 mg, 0.06 mol) and cesium carbonate (60 mg, 0.18 mmol) in DMF (2 mL) was stirred at 100 ° C. for 2 hours. The reaction mixture was extracted with ethyl acetate (3 × 30 mL). The combined organic phases were washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 2) to give the crude compound (16 mg) as a pale yellow solid, then further purified by preparative HPLC to give the desired product (5 mg, 24.6% ) Was obtained as a pale yellow solid.

Compound 3.3

Step A: (±) -exo-5-((1-((2- (trimethylsilyl) ethoxy) methyl) -1H-pyrrolo [2,3-b] pyridin-4-yl) oxy) -1a, 6b- Dihydro-1H-cyclopropa [b] benzofuran-1-carboxylate ethyl
Ethyl 5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylate (compound from step C in the synthesis of compound 2.1, 100 mg, 0.46 mmol), 4-bromo in toluene (5 mL) -1-((2- (trimethylsilyl) ethoxy) methyl) -1H-pyrrolo [2,3-b] pyridine (180 mg, 0.46 mol), K ₂ CO ₃ (158 mg, 1.15 mmol), Pd ₂ (dba) ₃ A mixture of (catalytic amount) and X-PHOS (22 mg, 0.046 mmol) was degassed and stirred in a microwave at 130 ° C. for 60 minutes. The mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 4) to give the title compound (120 mg, 56%) as a yellow oil.

Step B: (±) -exo-5-((1-((2- (trimethylsilyl) ethoxy) methyl) -1H-pyrrolo [2,3-b] pyridin-4-yl) oxy) -1a, 6b- Dihydro-1H-cyclopropa [b] benzofuran-1-carboxylic acid
Aqueous sodium hydroxide (0.54 mL, 2M, 1.08 mmol) was added to a stirred solution of the product from Step A (125 mg, 0.27 mmol) in THF (4 mL) and methanol (4 mL) at room temperature. The mixture was stirred at 60 ° C. for 10 minutes. The solvent was removed under reduced pressure and the residue was dissolved in water (5 mL). The solution was neutralized with HCl (2 mol / L) to pH = 7 and extracted with ethyl acetate (3 × 30 mL). The combined organic phases were washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (120 mg, 100%) which was used in the next step. Used directly.

Step C: (±) -Exo-6- (trifluoromethyl) -2- (5-((1-((2- (trimethylsilyl) ethoxy) methyl) -1H-pyrrolo [2,3-b] pyridine- 4-yl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-yl) -1H-benzo [d] imidazole
To a solution of the product from Step B (120 mg, 0.28 mmol) in DMF (5 mL) was added DIPEA (0.049 mL, 0.33 mmol) and HATU (115 mg, 0.31 mmol). After stirring for 30 minutes, 4- (trifluoromethyl) benzene-1,2-diamine (53.4 mg, 0.31 mmol) was added overnight at room temperature. The mixture was stirred overnight. The reaction mixture was extracted with ethyl acetate (3 × 20 mL), washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

The crude product was dissolved in acetic acid (5 mL) and the mixture was stirred at 60 ° C. for 6 hours. The mixture was extracted with EtOAc (3 × 20 mL), basified with aqueous NaHCO ₃ solution, washed with brine (10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 2) to give the title compound (100 mg, 63%) as a white solid. MS: M / e 579 (M + 1) ⁺ .

Step D: (±) -exo-2- (5-((1H-pyrrolo [2,3-b] pyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1 -Il) -6- (Trifluoromethyl) -1H-benzo [d] imidazole (Compound 3.3)
A solution of the product from Step C (43 mg, 0.074 mmol), ethane-1,2-diamine (0.5 mL) and a solution of TBAF in THF (0.3 mL, 1.0 M) in THF (10 mL) under N ₂ atmosphere Under stirring at 60 ° C. After stirring for 10 hours, additional TBAF in THF (0.5 mL, 1.0 M) was added and stirred for an additional 10 hours. The reaction mixture was treated with EtOAc (10 mL), washed with brine, dried over Na ₂ SO ₄ , concentrated and purified by preparative TLC (EtOAc: PE = 1: 2) to give the title product (20 mg , 60.3%) was obtained as a white solid.

Compound 3.4: (±) -exo-6- (trifluoromethyl) -2- (5-((2- (4- (trifluoromethyl) -1H-imidazol-2-yl) pyridin-4-yl) oxy ) -1a, 6b-Dihydro-1H-cyclopropa [b] benzofuran-1-yl) -1H-benzo [d] imidazole

Step A: (±) -Exo-5-((2-cyanopyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate
Ethyl 5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylate (compound from step C in the synthesis of compound 2.1, 100 mg, 0.46 mmol), 4-chloro in DMF (6 mL) A mixture of picolinonitrile (64 mg, 0.46 mol) and cesium carbonate (450 mg, 1.3 mmol) was stirred at 100 ° C. for 120 minutes. The reaction mixture was extracted with ethyl acetate (3 × 30 mL). The combined organic phases were washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 3) to give the title compound (100 mg, 67.5%) as a white solid.

Step B: (±) -Exo-5-((2-carbamimidoylpyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] methyl benzofuran-1-carboxylate
To a solution of the product from Step A (45 mg, 0.14 mmol) in methanol (3 mL), NaOMe (15 mg, 0.28 mmol) was added at room temperature. The mixture was stirred at room temperature for 3 hours. NH ₄ Cl (12 mg, 0.21 mmol) was then added at room temperature and the mixture was stirred for 1 hour and then at 60 ° C. for 1.5 hours. The solvent was removed and the residue was used directly in the next step without further purification.

Step C: (±) -exo-5-((2- (4- (trifluoromethyl) -1H-imidazol-2-yl) pyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cycloprop [b] Methyl benzofuran-1-carboxylate
To a solution of the crude product from Step B in CH ₃ CN (3 mL) was added K ₂ CO ₃ (78 mg, 0.56 mmol) and 3-bromo-1,1,1-trifluoropropan-2-one (32 mg, 0.17 mmol) was added at room temperature. The mixture was stirred at 60 ° C. for 2 hours. The solvent was removed under reduced pressure and the residue was dissolved in ethyl acetate (100 mL). The organic phase was washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 2) to give the title compound (10 mg, 17%) as a yellow solid.

Step D: (±) -exo-5-((2- (4- (trifluoromethyl) -1H-imidazol-2-yl) pyridin-4-yl) oxy) -1a, 6b-dihydro-1H-cycloprop [b] benzofuran-1-carboxylic acid
Aqueous sodium hydroxide (0.28 mL, 2M, 0.56 mmol) was added to a stirred solution of the product from Step C (60 mg, 0.14 mmol) in THF (1 mL) and methanol (1 mL) at room temperature. The mixture was stirred at 50 ° C. for 1 hour. The solvent was removed under reduced pressure and the residue was dissolved in CH ₂ Cl ₂ (20 mL) and water (5 mL). The solution is neutralized with HCl (2 mol / L) to pH = 7, extracted with CH ₂ Cl ₂ (3 × 30 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the title compound ( 40 mg, 72%) was obtained as a yellow solid, which was used directly in the next step. MS: M / e 404 (M + 1) ⁺ .

Step E: (±) -Exo-6- (trifluoromethyl) -2- (5-((2- (4- (trifluoromethyl) -1H-imidazol-2-yl) pyridin-4-yl) oxy ) -1a, 6b-Dihydro-1H-cyclopropa [b] benzofuran-1-yl) -1H-benzo [d] imidazole (compound 3.4)
Solution of the product from Step D (40 mg, 0.1 mmol), 4- (trifluoromethyl) benzene-1,2-diamine (19 mg, 0.11 mmol), DIPEA (0.02 mL, 0.12 mmol) in DMF (2 mL) To HATU (42 mg, 0.11 mmol) was added. The mixture was stirred at room temperature for 8 hours. The reaction mixture was extracted with ethyl acetate (3 × 30 mL), washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

The crude product was dissolved in acetic acid (2 mL) and stirred at 60 ° C. for 2 hours. The solvent was removed and the residue was purified by preparative HPLC to give the title compound (5.1 mg, 10%) as a white solid.

Compound 3.5: (±) -exo-6-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran -5-yl) oxy) -9H-purine

Stage A: (±) -exo-5-((9-((2- (trimethylsilyl) ethoxy) methyl) -9H-purin-6-yl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] Benzofuran-1-carboxylate
Ethyl 5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylate (compound from step C in the synthesis of compound 2.1, 61 mg, 0.28 mmol), 6-chloro in toluene (5 mL) -9-((2- (trimethylsilyl) ethoxy) methyl) -9H-purine (80 mg, 0.28 mol), K ₂ CO ₃ (96.6 mg, 0.70 mmol), Pd ₂ (dba) ₃ (catalytic amount), X- A mixture of PhOS (13.3 mg, 0.028 mmol) was degassed and stirred in a microwave at 130 ° C. for 2.5 hours. The mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 2) to give the title compound (73 mg, 56%) as a colorless oil. MS: M / e 469 (M + 1) ⁺ .

Step B: (±) -exo-5-((9-((2- (trimethylsilyl) ethoxy) methyl) -9H-purin-6-yl) oxy) -1a, 6b-dihydro-1H-cyclopropa [b] Benzofuran-1-carboxylic acid
A solution of the product from Step A (65 mg, 0.14 mmol), aqueous sodium hydroxide (0.28 mL, 2M, 0.56 mmol) and THF (4 mL) was stirred and refluxed for 6 hours. The solvent was removed under reduced pressure and the residue was neutralized with HCl (2 mol / L) to pH = 4-5 and extracted with ethyl acetate (3 × 30 mL). The combined organic phases were washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (62 mg, 100%), which in the next step Used directly.

Step C: (±) -exo-6-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran -5-yl) oxy) -9-((2- (trimethylsilyl) ethoxy) methyl) -9H-purine
To a solution of the product from Step B (62 mg, 0.14 mmol) in DMF (5 mL) was added DIPEA (21.7 mg, 0.188 mmol) and HATU (58.5 mg, 0.154 mmol). After stirring for 0.5 hour, (trifluoromethyl) benzene-1,2-diamine (27.1 mg, 0.15 mmol) was added and the mixture was stirred at room temperature overnight. Water was added and the mixture was extracted with ethyl acetate (3 × 20 mL). The combined organic phases were washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

The crude product was dissolved in acetic acid (5 mL) and stirred at 60 ° C. for 5 hours. The mixture was extracted with EtOAc (3 × 20 mL). The combined organic phases were washed with aqueous Na2CO3 solution and brine (10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc) to give the title compound (50 mg, 61.4%) as a white solid. MS: M / e 581 (M + 1) ⁺ .

Stage D: (±) -exo-6-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran -5-yl) oxy) -9H-purine (compound 3.5)
To a solution of the product from Step C (50 mg, 0.086 mmol), ethane-1,2-diamine (0.5 mL) in THF (10 mL), add a solution of TBAF in THF (0.3 mL, 1.0 M) to N _2. Added under atmosphere. The mixture was stirred at 60 ° C. for 5 hours. The reaction mixture was treated with EtOAc (20 mL), washed with brine, dried over Na ₂ SO ₄ , filtered and concentrated. The residue was purified by preparative TLC (CH ₂ Cl ₂ : MeOH = 10: 1) to give the title product (10 mg, 26%) as a white solid.

Compound 3.6: (±) -exo-2- (5- (pyridin-4-yloxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-yl) -6- (trifluoromethyl) -1H -Benzo [d] imidazole

Step A: (±) -Exo-5- (pyridin-4-yloxy) -1a, 6b-dihydro-1H-cyclopropa [b] ethyl benzofuran-1-carboxylate
Ethyl 5-hydroxy-1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylate (compound from step C in the synthesis of compound 2.1, 150 mg, 0.69 mmol), 4-bromo in DMF (5 mL) A mixture of pyridine hydrochloride (134 mg, 0.69 mol), cesium carbonate (681 mg, 2.07 mmol) and copper powder (catalytic amount) was stirred in a microwave at 150 ° C. for 30 minutes. The reaction mixture was filtered and the filtrate was diluted with water (20 mL) and extracted with ethyl acetate (5 × 10 mL). The combined organic phases were washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 2) to give the title compound (20 mg, 10%) as a colorless oil. MS: M / e 298 (M + 1) ⁺ .

Step B: (±) -Exo-5- (pyridin-4-yloxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-carboxylic acid
A mixture of the product from Step A (20 mg, 0.077 mmol) and aqueous sodium hydroxide (0.15 mL, 2M, 0.30 mmol) in THF (4 mL) was stirred at 60 ° C. for 5 hours. The solvent was removed under reduced pressure and the mixture was neutralized with HCl (2 mol / L) to pH = 7 and concentrated under reduced pressure to give the title crude compound, which was used directly in the next step.

Step C: (±) -exo-2- (5- (pyridin-4-yloxy) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-1-yl) -6- (trifluoromethyl) -1H -Benzo [d] imidazole (Compound 3.6)
To a solution of the product from Step B (0.077 mmol) in DMF (3 mL) was added DIPEA (20 mg, 0.155 mmol) and HATU (32.4 mg, 0.0853 mmol). After 30 minutes 4- (trifluoromethyl) benzene-1,2-diamine (15 mg, 0.0853 mmol) was added and the mixture was stirred at room temperature overnight. Water was added and the mixture was extracted with ethyl acetate (3 × 20 mL). The combined organic phases were washed with brine (2 × 10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

The crude product was dissolved in acetic acid (4 mL) and the mixture was refluxed at 60 ° C. for 6 hours. The mixture was extracted with EtOAc (3 × 20 mL). The combined organic phases were washed with aqueous Na ₂ CO ₃ solution and brine, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (10 mg, 31.6%, 3 steps) as a white solid.

Compound 3.7: (±) -exo-5-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-H-cyclopropa [b] benzofuran -5-yl) oxy) -1H-pyrido [2,3-d] [1,3] oxazin-2 (4H) -one

Stage A: tert-butyl (4-fluoro-3-formylpyridin-2-yl) carbamate
Stir a solution of tert-butyl 4-fluoropyridin-2-ylcarbamate (1 g, 4.72 mmol) in THF (20 mL) and add n-BuLi (4.7 mL, 11.8 mmol) dropwise at −78 ° C. did. After stirring for 0.5 h, a solution of DMF (2 mL) in THF (2 mL) was added dropwise at -78 ° C. TLC showed completion of reaction. The reaction was stopped with 1N HCl at −78 ° C. to pH = 4. Water (20 mL) was then added and extracted with ethyl acetate (3 × 20 mL). The combined organic phases were washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by column chromatography (EtOAc: PE = 1: 2) to give the title compound (0.65 g, 57%) as a white solid.

Step B: tert-butyl 4-fluoro-3- (hydroxymethyl) pyridin-2-ylcarbamate
To a solution of the product of Step A (480 mg, 2 mmol) in MeOH (3 mL), NaBH ₄ (76 mg, 2 mmol) was added at 0 ° C. The reaction mixture was stirred at 0 ° C. for 30 minutes. The reaction was quenched with saturated NH ₄ Cl (1 mL) and water (5 mL) and extracted with ethyl acetate (2 × 15 mL). The combined organic phases were dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the title compound (460 mg, 95%) as a white solid, which was used directly in the next step.

Step C: tert-butyl 3- (bromomethyl) -4-fluoropyridin-2-ylcarbamate
CBr ₄ (531 mg, 1.6 mmol) was added to a solution of the product of Step B (242 mg, 1 mmol) in THF (3 mL). A solution of triphenylphosphine in THF (1 mL) was then added dropwise and the mixture was stirred at room temperature for 3 hours. The mixture was loaded onto a silica gel column. Elution with (EtOAc: PE = 1: 3) gave the title compound (160 mg, 52%) as a white solid.

Step D: 5-Fluoro-1H-pyrido [2,3-d] [1,3] oxazin-2 (4H) -one
A solution of the product of Step C (120 mg, 0.4 mmol) in DMSO (1 mL) was stirred at 60 ° C. for 4 hours under N ₂ . Water (10 mL) was then added and extracted with ethyl acetate (3 × 15 mL). The combined organic phases were washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative TLC (EtOAc: PE = 1: 1) to give the title compound (20 mg, 30%) as a solid.

Step E: (±) -exo-5-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran -5-yl) oxy) -1H-pyrido [2,3-d] [1,3] oxazin-2 (4H) -one
A mixture of Step 3.2 product of Compound 3.2 (16 mg, 0.1 mmol), Step D product (33 mg, 0.1 mmol) and Cs ₂ CO ₃ (98 mg, 0.3 mmol) in DMF (2 mL) at 110 ° C. 3 Stir for hours. The mixture was cooled to room temperature, water (10 mL) was added and extracted with ethyl acetate (3 × 15 mL). The combined organic phases were washed with brine, dried over anhydrous sodium sulfate and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (7 mg, 15%) as a white solid.

Compound 3.8: (±) -exo-6-chloro-5-((1- (6- (trifluoromethyl) -1H-benzo [d] imidazol-2-yl) -1a, 6b-dihydro-1H-cyclopropa [b] benzofuran-5-yl) oxy) -3,4-dihydro-1,8-naphthyridin-2 (1H) -one
To a stirred solution of compound 2.2 (70 mg, 0.15 mmol) in AcOH (2 mL) was added NCS (19 mg, 0.15 mmol) at ambient temperature and the mixture was heated at 60 ° C. for 3 h. The mixture was concentrated under reduced pressure, and 2 mL of 2N NaOH aqueous solution was added to the residue, followed by extraction with EtOAc (2 mL × 3). The combined extracts were washed with brine (2 mL × 3), dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by preparative TLC to give the title compound (9 mg, 12%) as a white solid.

Raf IC ₅₀ Assay Protocol Compounds disclosed herein can be tested in a time-resolved fluorescence energy transfer assay against B-Raf (V600E) (PV3849, Invitrogen) or C-Raf (Y340D / Y341D) (PV3805, Invitrogen) Tested. Assay, 0.0625nM B-Raf or 0.5 nM C-Raf, 25 mM Tris _{pH7.4,10mM MgCl 2, 0.5mM EGTA, 0.5mM} Na 3 BO 4, 5mM beta - glycerophosphate, 0.01% Triton X-100,2.5 mM DTT, 0.1% BSA, 0.1 mM ATP, 13.7 nM GST-labeled MEK1 (full-length protein with K97R mutation, recombinant protein purified from bacterial expression system) and 0-5 μM of the compound disclosed herein (final concentration) 1% DMSO) was performed in the reaction mixture (10 μL). The enzyme was incubated with the compound for 60 minutes at room temperature and the reaction was initiated by the addition of ATP and GST-MEK1. After incubation at room temperature for 60 minutes, 25 mM Tris pH 7.4, 400 mM KF, 50 mM EDTA, 0.01% BSA, 0.01% Triton X-100, Eu3 + cryptate conjugated rabbit polyclonal antibody anti-phospho MEK1 / 2 (Ser217 / 221) The reaction was stopped by adding the same amount of stop buffer containing one test and one test of d2 conjugated mouse monoclonal antibody anti-glutathione S-transferase. The plate was sealed and incubated for 2 hours at room temperature, then the TR-FRET signal was read on a BMG PHERAstar FS instrument. The IC ₅₀ for each compound was calculated by non-linear regression with Graphpad Prism software.

Compounds 1.1-1.3, 2.1-2.37 and 3.1-3.8 inhibited B-Raf (V600E) / C-Raf with IC ₅₀ values ranging from 0.1 nM to 10 μM.

(Table 1) IC ₅₀

Claims (9)

Barrett's adenocarcinoma, breast cancer, cervical cancer, bile duct cancer, glioblastoma, colorectal cancer containing a compound of formula (I), a stereoisomer thereof, or a pharmaceutically acceptable salt thereof Treatment for stomach cancer, lung cancer, ovarian cancer, pancreatic cancer, prostate cancer, blood cancer, melanoma, histiocytic lymphoma, lung adenocarcinoma, small cell lung cancer, or thyroid cancer:

Where
Q is selected from C and N;
W is selected from C and N;
X is selected from CH ₂ and O;
Y is selected from NR ¹² , O, and S;
Z is selected from O, S, NR ¹³ , CO, SO, SO ₂ , and CR ¹³ R ¹⁴ ;
R ¹ , R ² , R ³ , R ⁴ , R ⁵ , and R ⁶ may be the same or different and are each hydrogen, halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, Alkynyl, -CN, -NR ¹³ R ¹⁴ , -OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ ,- Selected from NR ¹³ CONR ¹⁴ R ¹⁵ , -NR ¹³ CO ₂ R ¹⁴ , -SO ₂ R ¹³ , -NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , -NR ¹³ SO ₂ R ¹⁴ , and -NR ¹³ SO ₂ aryl, Wherein alkyl, alkenyl, alkynyl, cycloalkyl, heteroaryl, aryl, and heterocyclyl may be substituted with at least one substituent R ¹⁶ or (R ¹ and R ² ), and / or (R ³ and R ^4), and / or (R ⁵ and R ^6), together with the ring to which they are attached, at least one Substituted with a substituent R ¹⁶ form a fused ring selected from heterocyclyl and heteroaryl ring; provided that when Q is N, if R ¹ is absent, and W is N, R ⁵ is not present;
R ⁷ is selected from hydrogen, halogen, alkyl, —O-alkyl, and —S-alkyl;
R ¹² is selected from hydrogen and alkyl;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings. The therapeutic agent according to claim 1, wherein the compound of the formula (I) is a compound of the formula (II):

Where
Q is selected from C and N;
X is selected from CH ₂ and O;
Y is selected from NR ¹² , O, and S;
Z is selected from O, S, NR ¹³ , CO, SO, SO ₂ , and CR ¹³ R ¹⁴ ;
R ¹ , R ² , R ³ , R ⁴ , R ⁸ , R ⁹ , R ¹⁰ and R ¹¹ may be the same or different and are each hydrogen, halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, Heterocyclyl, heteroaryl, alkynyl, -CN, -NR ¹³ R ¹⁴ , -OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ , -NR ¹³ CONR ¹⁴ R ¹⁵ , -NR ¹³ CO ₂ R ¹⁴ , -SO ₂ R ¹³ , -NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , and -NR ¹³ SO ₂ R ¹⁴ Alkyl, alkenyl, alkynyl, cycloalkyl, heteroaryl, aryl, and heterocyclyl may each be substituted with at least one substituent R ¹⁶ , or (R ¹ and R ² ), and / or (R ³ and R ⁴ ), and / or (R ⁸ and R ⁹ ), and / or (R ⁹ and R ¹⁰ ), and / or (R ¹⁰ and R ¹¹ ) together with the ring to which they are attached form a fused ring selected from heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ ; When is N, R ¹ is absent;
R ⁷ is selected from hydrogen, halogen, alkyl, —O-alkyl, and —S-alkyl;
R ¹² is selected from hydrogen and alkyl;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings. The therapeutic agent according to claim 1, wherein the compound of the formula (I) is a compound of the formula (III):

Where
X is selected from CH ₂ and O;
Z is selected from O, S, NR ¹³ , CO, SO, SO ₂ , and CR ¹³ R ¹⁴ ;
A is _{-CH 2 -, - CH 2 CH} 2 -, - CH = CH -, = CH -, - NR 13 -, - CH 2 -O -, - O-, and is selected from -S-;
M is selected from = CH- and -C (O)-; or M is absent;
R ¹ , R ² , R ⁸ , R ⁹ , R ¹⁰ and R ¹¹ may be the same or different and are each hydrogen, halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, alkynyl , -CN, -NR ¹³ R ¹⁴ , -OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ , -NR ¹³ CONR ¹⁴ R ¹⁵ , —NR ¹³ CO ₂ R ¹⁴ , —SO ₂ R ¹³ , —SO ₂ aryl, —NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , and —NR ¹³ SO ₂ R ¹⁴ , where alkyl , Alkenyl, alkynyl, cycloalkyl, heteroaryl, aryl, and heterocyclyl may each be substituted with at least one substituent R ¹⁶ ,
Or (R ¹ and R ² ) and / or (R ⁸ and R ⁹ ) and / or (R ⁹ and R ¹⁰ ) and / or (R ¹⁰ and R ¹¹ ) are the rings to which they are attached Together with a fused ring selected from heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ ;
R ¹² is selected from hydrogen and alkyl;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings. The therapeutic agent according to claim 1, wherein the compound of the formula (I) is a compound of the formula (IV):

Where
B is selected from CH ₂ , O, and NR ¹³ ;
X is selected from CH ₂ and O;
R ¹ , R ² , R ⁸ , R ⁹ , R ¹⁰ and R ¹¹ may be the same or different and are each hydrogen, halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, alkynyl , -CN, -NR ¹³ R ¹⁴ , -OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ , -NR ¹³ CONR ¹⁴ R ¹⁵ , —NR ¹³ CO ₂ R ¹⁴ , —SO ₂ R ¹³ , —SO ₂ aryl, —NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , and —NR ¹³ SO ₂ R ¹⁴ , where alkyl , Alkenyl, alkynyl, cycloalkyl, heteroaryl, aryl, and heterocyclyl may each be substituted with at least one substituent R ¹⁶ or (R ¹ and R ² ) and / or (R ⁸ and R ⁹ ), and / or (R ⁹ and R ^10), and / or (R ¹⁰ and R ^11), they are sintered With to that ring, form a fused ring selected from at least one of which may be substituted with a substituent R ¹⁶ heterocyclyl and heteroaryl ring;
R ¹² is selected from hydrogen and alkyl;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings. The therapeutic agent according to claim 1, wherein the compound of the formula (I) is a compound of the formula (V):

Where
X is selected from CH ₂ and O;
R ⁸ , R ⁹ , R ¹⁰ and R ¹¹ may be the same or different and are each hydrogen, halogen, alkyl, alkenyl, cycloalkyl, aryl, heterocyclyl, heteroaryl, alkynyl, -NR ¹³ R ¹⁴ ,- OR ¹³ , -COR ¹³ , -CO ₂ R ¹³ , -CONR ¹³ R ¹⁴ , -C (= NR ¹³ ) NR ¹⁴ R ¹⁵ , -NR ¹³ COR ¹⁴ , -NR ¹³ CONR ¹⁴ R ¹⁵ , -NR ¹³ CO ₂ R ¹⁴ , —SO ₂ R ¹³ , —SO ₂ aryl, —NR ¹³ SO ₂ NR ¹⁴ R ¹⁵ , and —NR ¹³ SO ₂ R ¹⁴ , wherein alkyl, alkenyl, alkynyl, cycloalkyl, heteroaryl, Aryl and heterocyclyl may each be substituted with at least one substituent R ¹⁶ or (R ⁸ and R ⁹ ) and / or (R ⁹ and R ¹⁰ ) and / or (R ¹⁰ and R ¹¹ ) Is substituted with at least one substituent R ¹⁶ together with the ring to which they are attached Forming a fused ring selected from optionally heterocyclyl and heteroaryl rings;
R ¹³ , R ¹⁴ and R ¹⁵ may be the same or different and are each selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl; or (R ¹³ And R ¹⁴ ) and / or (R ¹⁴ and R ¹⁵ ) are selected from the heterocyclyl and heteroaryl rings optionally substituted with at least one substituent R ¹⁶ together with the atoms to which they are attached. Each forming a ring;
R ¹⁶ is halogen, haloalkyl, alkyl, alkenyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, alkynyl, oxo, -CN, -OR ', - NR'R '', - COR', - CO 2 R ', - CONR'R '', -C (= NR ') NR''R''',-NR'COR'',-NR'CONR'R'',-NR'CO ₂ R '', -SO ₂ R ', -SO ₂ aryl, -NR'SO ₂ NR''R''',NR'SO ₂ R '', and -NR'SO ₂ aryl, where R ', R'', and R '''Is independently selected from H, haloalkyl, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, and heteroaryl, or (R' and R '') and / or (R '' and R ''') Together with the atoms to which they are attached form a ring selected from heterocyclyl and heteroaryl rings. The compound of the above formula (I) is the following compound:


The therapeutic agent according to claim 1, wherein The compound of the above formula (I) is the following compound:
The therapeutic agent according to claim 1, wherein The compound of the above formula (I) is the following compound:
The therapeutic agent according to claim 7, wherein The therapeutic agent according to any one of claims 1 to 8, in combination with at least one other therapeutic agent.