JP6151695B2 - Nucleic acid chromatography test tool and method for producing the same - Google Patents

Description

  The present invention relates to a nucleic acid chromatography test tool. More specifically, the present invention relates to a nucleic acid chromatography test tool having a low content of a nucleic acid probe in a capture nucleic acid solid phase and having good visibility of a display portion that is a surface portion of the capture nucleic acid solid phase, and a method for producing the same.

  By examining the presence or absence of nucleic acids (DNA, RNA) derived from viruses or bacteria, the presence or absence of nucleic acids derived from mutated genes related to specific diseases or constitutions, hereditary diseases such as infections and tumors, constitutions, etc. It is possible to diagnose accurately. For example, when diagnosing the presence or absence of virus infection, a mucous membrane or the like is collected from a patient, and a process of amplifying only the nucleic acid derived from the virus using the PCR method is performed. If a nucleic acid derived from a virus is detected in the sample obtained by this treatment, it can be determined that the patient is infected with the virus. Today, in addition to the accumulation of information on the nucleotide sequence of nucleic acids as a result of the development of genomic analysis, the PCR method has been improved to selectively amplify nucleic acids derived from viruses and bacteria and nucleic acids derived from specific mutant genes. It is easy to do. Therefore, a technique for simply determining the presence or absence of a specific nucleic acid is required in order to more generally spread the application of the above-described diagnostic method for nucleic acids.

  As a technique for easily determining the presence or absence of a specific nucleic acid (hereinafter referred to as “target nucleic acid”), a nucleic acid chromatography test tool has been proposed (Patent Documents 1 and 2). A method for detecting a target nucleic acid using this nucleic acid chromatography test tool is as follows. First, a liquid sample is developed in a porous sheet that constitutes a nucleic acid chromatography tester by the same mechanism as paper chromatography. And the target nucleic acid contained in a sample is captured by the nucleic acid probe fixed to the surface of the porous sheet. And the detection of the target nucleic acid is displayed on the surface of the porous sheet by coloring the captured target nucleic acid. That is, in the nucleic acid chromatography test tool, when the target nucleic acid is contained in the sample, an indication appears on the surface of the porous sheet. On the other hand, when the target nucleic acid is not contained in the sample, the porous sheet No indication appears on the surface. The nucleic acid chromatography test tool can determine the presence or absence of the target nucleic acid with such a very simple mechanism.

  In general, a nucleic acid chromatography test tool is widely used in the form of a test piece (usually referred to as a “strip”) made by attaching an elongated rectangular porous sheet to an elongated rectangular mount (backing member). (Patent Documents 3 and 4). For example, if the target nucleic acid was previously labeled with a dye when preparing the sample, insert the tip of the above-mentioned strip into the test tube containing the sample, and then perform any operation. Even if not performed, the sample develops in the porous sheet by capillary action. The target nucleic acid in the sample is captured and accumulated in the porous sheet at the portion where the nucleic acid probe is fixed (display portion), so that the surface of the porous sheet (display portion) can be colored. In this way, the presence or absence of the target nucleic acid can be determined by very simple handling in which only the tip of the strip is inserted into the test tube containing the sample. Therefore, when a nucleic acid chromatography test tool is used, there is an advantage that a large number of samples can be tested simultaneously.

JP 2001-157598 A JP 2005-503556 A JP 2006-201062 A JP 2010-14507 A

  The above-described nucleic acid chromatography test tool (chromatography strip) includes a porous sheet having a display portion on the test surface. The porous sheet has a sheet-like porous substrate on which the nucleic acid probe is fixed, and the surface of the nucleic acid probe (capture nucleic acid solid phase) fixed on the porous substrate (exposed portion on the test surface). Is the display.

  The nucleic acid probe can be immobilized by applying a solution containing the nucleic acid probe (nucleic acid probe-containing solution) to the porous substrate and drying it. When a solution containing a nucleic acid probe is applied to a porous substrate, the solution containing the nucleic acid probe penetrates into the porous substrate, and a phase (layer) containing the nucleic acid probe is formed in the porous substrate. At this time, the nucleic acid probe-containing solution penetrates deep into the porous substrate. Then, the nucleic acid probe-containing solution enters the porous substrate, and the nucleic acid probe is fixed in the porous substrate to form a capture nucleic acid solid phase. As a result, the surface of the capture nucleic acid solid phase (the portion exposed to the test surface) becomes the display unit.

  Thus, when the nucleic acid probe-containing solution penetrates deep into the porous substrate and the capture nucleic acid solid phase is formed deep into the porous substrate, the deeply penetrated part is Does not contribute to the visibility. For this reason, there has been a problem that the nucleic acid probe has been wasted for the deep penetration. And despite the large amount of nucleic acid probes used, there was a problem that the visibility of the display part was not improved.

  In general, color development involves binding of a color developing material consisting of particles larger than the nucleic acid probe or target nucleic acid to the target nucleic acid, and the target nucleic acid is captured by the nucleic acid probe in the porous sheet, resulting in aggregation of the color developing material. This is visually recognized. Therefore, the colored nucleic acid probe solid phase prevents the subsequent flow of the target nucleic acid to be captured by the target nucleic acid, particularly the nucleic acid probe downstream of the nucleic acid probe. As a result, when detecting multiple (plural types) nucleic acids, there is a problem in that the detection sensitivity is deteriorated and the detection time is extended on the downstream side in the direction in which the sample solution is developed.

  The present invention has been made to solve such problems. The main object of the present invention is to provide a nucleic acid chromatography test tool having a low content of nucleic acid probes in a capture nucleic acid solid phase and a method for producing the same. Furthermore, the main object of the present invention is to provide a nucleic acid chromatography test tool and a method for producing the same, in which the visibility of the display part which is the surface part of the capture nucleic acid solid phase (the part exposed on the test surface) is good. . Furthermore, the main object of the present invention is to provide a nucleic acid chromatography test tool capable of efficiently detecting a plurality of types of nucleic acids even in the detection of multiple (plural types of) nucleic acids and a method for producing the same.

  In order to solve the above-described problems, the present invention provides the following nucleic acid chromatography test tool and a method for producing the same.

[1] A porous sheet that is long in one direction on which a sample containing a target nucleic acid can be developed, and the porous sheet has a sheet-like porous substrate having one surface as a display portion forming surface. The porous substrate has a capture nucleic acid solid phase formed such that a nucleic acid probe for capturing a target nucleic acid penetrates from the display portion formation surface and is partially exposed to the display portion formation surface. The portion of the capture nucleic acid solid phase exposed on the display portion forming surface of the porous substrate is a display portion, and the display portion is a strip extending at an angle intersecting the longitudinal direction of the porous sheet, The nucleic acid chromatography test tool, wherein the concentration of the nucleic acid probe is higher as the capture nucleic acid solid phase is closer to the display unit in the thickness direction.

[2] The nucleic acid chromatography test device according to [1], wherein the thickness of the capture nucleic acid solid phase is non-uniform and less than 1 times the thickness of the porous sheet.

[3] The nucleic acid chromatography test tool according to [1] or [2], further comprising a sheet-like backing member disposed on the other surface of the porous substrate.

[4] The nucleic acid chromatography test tool according to any one of [1] to [3], wherein the porous sheet is a multi-layer sheet.

[5] above [1] to [4] it is any method for producing a nucleic acid chromatographic test device according to the, one which is the display portion forming surface of the unidirectional long sheet of the porous substrate On the surface, a coating nucleic acid solution is applied in a strip shape by a plurality of discharges of a micropipette and permeated into the porous substrate, and the capture nucleic acid solution is 0.5 to 5 at 20 to 50 ° C. dried time, the production method of the nucleic acid chromatographic test device having a drying step of forming the capture nucleic acid solid phase.
[ 6 ] The method for producing a nucleic acid chromatography test tool according to [5], wherein the discharge amount of the capture nucleic acid solution by one discharge operation of the micropipette is 0.02 to 0.4 μl.

[ 7 ] The application step includes at least one substrate on which a cavity filled with the capture nucleic acid solution is formed, and a piezoelectric / electrostrictive element disposed on at least one wall surface of the substrate forming the cavity. with the door, by the micropipette that is configured to the capture nucleic acid solution in the cavity is discharged by operation of the piezoelectric / electrostrictive element, said capture nucleic acid solution on the detection surface of the porous substrate The method for producing a nucleic acid chromatography test tool according to the above [5] or [6] , which is a step of applying in a strip shape and permeating the porous substrate.

  In the nucleic acid chromatography test tool of the present invention, the concentration of the nucleic acid probe in the capture nucleic acid solid phase is higher in the thickness direction as it is closer to the display unit. Thus, since the concentration of the nucleic acid probe in the solid phase of the capture nucleic acid is higher in the thickness direction as it is closer to the display portion, the capture nucleic acid can be obtained even if the content of the nucleic acid probe in the capture nucleic acid solid phase is small. The visibility of the display portion which is the surface portion of the solid phase is good. Furthermore, since the concentration of the nucleic acid probe in the capture nucleic acid solid phase is higher as it is closer to the display part, the concentration of the nucleic acid probe at a deep position in the thickness direction (thickness direction of the porous sheet) of the capture nucleic acid solid phase is Therefore, the content of the nucleic acid probe is reduced accordingly. In addition, since the concentration of the nucleic acid probe at a deep position is low, even when the target nucleic acid is captured on the upstream capture nucleic acid solid phase, the target nucleic acid to be captured with the nucleic acid probe on the downstream capture nucleic acid solid phase is It can preferentially pass through a portion (deep position) where the probe concentration is low. As a result, the flow of the target nucleic acid is not hindered. As a result, when detecting multiple (plural types) nucleic acids, the detection sensitivity is deteriorated or the detection time is extended on the downstream side in the direction in which the sample liquid is developed. Can be avoided.

It is a top view which shows typically one Embodiment of the nucleic acid chromatography test | inspection tool of this invention. It is a side view which shows typically one Embodiment of the nucleic acid chromatography test | inspection tool of this invention. It is a schematic diagram which shows a part of cross section which is parallel to a longitudinal direction and perpendicular | vertical to a detection surface of one Embodiment of the nucleic acid chromatography test | inspection tool of this invention. It is a schematic diagram which shows the cross section of the micropipette used for one Embodiment of the manufacturing method of the nucleic acid chromatography test | inspection tool of this invention. It is a schematic diagram which shows the cross section of the dispensing apparatus used for one Embodiment of the manufacturing method of the nucleic acid chromatography test | inspection tool of this invention formed by arranging two or more micropipettes.

  Hereinafter, embodiments of the present invention will be specifically described with reference to the drawings. The present invention is not limited to the following embodiments. It should be understood that modifications and improvements as appropriate to the following embodiments are also included in the scope of the present invention based on ordinary knowledge of those skilled in the art without departing from the spirit of the present invention. It is.

(1) Nucleic acid chromatography test tool:
As shown in FIGS. 1 to 3, one embodiment of the nucleic acid chromatography test tool of the present invention (nucleic acid chromatography test tool 1) can develop a “sample containing a target nucleic acid” in one direction. A long porous sheet 10 is provided. The porous sheet 10 has a sheet-like porous substrate 11 having one surface as a display portion forming surface 12. The porous substrate 11 is formed so that a nucleic acid probe for capturing a target nucleic acid penetrates from the display part forming surface 12 and is “exposed partly on the display part forming surface 12”. (16a, 16b). The portion of the capture nucleic acid solid phase 16 (16a, 16b) exposed on the display portion forming surface 12 of the porous substrate 11 is the display portion 15 (15a, 15b). And the display part 15 (15a, 15b) is a strip | belt shape extended in the angle which cross | intersects the longitudinal direction of the porous sheet 10. FIG. Furthermore, the thickness of the capture nucleic acid solid phase 16 (16a, 16b) is non-uniform. Furthermore, the thickness of the capture nucleic acid solid phase 16 (16a, 16b) is less than 1 times the thickness of the porous sheet 10. Furthermore, the concentration of the nucleic acid probe in the capture nucleic acid solid phase 16 (16a, 16b) is higher in the thickness direction as it is closer to the display unit 15 (15a, 15b). The sample containing the target nucleic acid is a liquid sample. In addition, the display portion forming surface 12 of the porous substrate 11 is used as a detection surface 13 in the porous sheet 10.

  FIG. 1 is a plan view schematically showing one embodiment of the nucleic acid chromatography test tool of the present invention. FIG. 2 is a side view schematically showing one embodiment of the nucleic acid chromatography test tool of the present invention. FIG. 3 is a schematic view showing a part of a cross section that is parallel to the longitudinal direction and perpendicular to the detection surface, in one embodiment of the nucleic acid chromatography test tool of the present invention.

  Thus, in the nucleic acid chromatography test tool 1 of the present embodiment, the concentration of the nucleic acid probe of the capture nucleic acid solid phase 16 is higher as it is closer to the display unit 15 in the thickness direction. The concentration of the nucleic acid probe at a deep position in the vertical direction is low. Therefore, the content of the nucleic acid probe in the capture nucleic acid solid phase 16 is reduced accordingly. Furthermore, since the concentration of the nucleic acid probe is higher as the capture nucleic acid solid phase 16 is closer to the display unit 15 in the thickness direction, the capture nucleic acid can be obtained even if the content of the nucleic acid probe in the capture nucleic acid solid phase 16 is small. The visibility of the display unit 15 which is the surface part of the solid phase 16 is good.

  In the nucleic acid chromatography test tool 1 of the present embodiment, the porous sheet 10 is a porous sheet that is long in one direction. The porous sheet 10 expands a liquid sample by immersing one end (sample collection unit 45) in the longitudinal direction in a sample (liquid sample) containing a target nucleic acid. In the nucleic acid chromatography test tool 1 of the present embodiment, the porous sheet 10 is formed so that one end in the longitudinal direction (sample collection part 45) is formed in a tapered shape so that even a small number of samples can be easily developed. ing. The shape of the porous sheet 10 is not particularly limited except that the shape is long in one direction. For example, the shape of the porous sheet 10 viewed from the detection surface side (the shape of the detection surface) is a rectangle, “a shape in which rectangular corners are curved”, and “a rectangular corner is chamfered linearly. Or the like ".

  The length in the longitudinal direction of the porous sheet 10 is preferably 15 to 100 mm, and particularly preferably 40 to 50 mm. If it is shorter than 15 mm, it may be difficult to detect the target nucleic acid in the porous sheet. When the length is longer than 100 mm, the length required for detecting the target nucleic acid is significantly exceeded, and it may be unnecessarily long. The length of the porous sheet 10 in the direction (width direction) orthogonal to the longitudinal direction and parallel to the detection surface 13 is preferably 2 to 8 mm, particularly preferably 3 to 5 mm. If it is shorter than 2 mm, it may be difficult to detect the target nucleic acid in the porous sheet. When the length is longer than 8 mm, the width necessary for detecting the target nucleic acid is significantly exceeded, and it may be unnecessarily long (thick). Further, the length (thickness) of the porous sheet 10 in the longitudinal direction and the direction (thickness direction) orthogonal to the detection surface 13 is preferably 0.05 to 0.5 mm, and preferably 0.2 to 0.3 mm. Particularly preferred. The porous sheet includes a thin sheet such as a membrane.

  In the nucleic acid chromatography test tool 1 of the present embodiment, the porous sheet 10 has a sheet-like porous base material 11 "with one surface as the display portion forming surface 12". In the nucleic acid chromatography test tool 1 of the present embodiment, the porous sheet 10 is obtained by forming the display unit 15 (capture nucleic acid solid phase 16) and the position marker 14 on the porous base material 11. Therefore, the shape of the porous sheet 10 and the shape of the porous substrate 11 are the same shape.

  Moreover, it is also a preferable aspect that the porous sheet 10 is a multi-layer sheet. When the porous sheet 10 is a sheet having a plurality of layers, the porous substrate 11 is a substrate having a plurality of layers. When the porous substrate 11 is a substrate having a plurality of layers, the porous substrate 11 preferably has 2 to 4 layers.

  Examples of the material of the porous substrate 11 include nitrocellulose, PET (polyethylene terephthalate), cellulose, nylon, PVDF (polyvinylidene fluoride), polyethersulfone, and the like. The average pore diameter of the porous substrate 11 is preferably 0.15 to 0.9 μm, and particularly preferably 0.25 to 0.65 μm. If it is smaller than 0.15 μm, it may be difficult to develop a sample containing the target nucleic acid. If it is larger than 0.9 μm, it may be difficult to separate the target nucleic acid from other nucleic acids. When the porous base material 11 is a base material having a plurality of layers, each layer is preferably made of the above material.

  In addition, the porous base material 11 has a capture nucleic acid anchor formed so that a nucleic acid probe for capturing a target nucleic acid penetrates from the display portion forming surface 12 and is “partially exposed on the display portion forming surface 12”. It has phase 16 (16a, 16b). And the part (part) exposed to the display part formation surface 12 of the porous base material 11 of the capture nucleic acid solid phase 16 (16a, 16b) is the display part 15 (15a, 15b). Since the display portion forming surface 12 of the porous substrate 11 is the detection surface 13 in the porous sheet 10, the porous sheet 10 has the display portion 15 on the detection surface 13. In other words, “the display unit 15 of the porous sheet 10 is exposed to the detection surface 13 of the capture nucleic acid solid phase 16 formed by the penetration of the nucleic acid probe for capturing the target nucleic acid from the detection surface 13”. It can also be said to be “part”.

  When the sample containing the target nucleic acid is developed with the porous sheet 10, the target nucleic acid in the sample is captured by the nucleic acid probe in the capture nucleic acid solid phase 16, and the supplemental nucleic acid solid phase 16 is colored. A target nucleic acid is detected. When the supplemental nucleic acid solid phase 16 is colored, the display unit 15 that is “the portion of the supplemental nucleic acid solid phase 16 exposed on the detection surface 13” is also colored, so the color of the display unit 15 is colored. The target nucleic acid can be detected by displaying such as. That is, the display unit 15 is a part that detects and displays the target nucleic acid.

  In the nucleic acid chromatography test tool 1 of the present embodiment, the display unit 15 (15a, 15b) has a strip shape extending at an angle that intersects the longitudinal direction of the porous sheet 10. As shown in FIG. 1, the angle between the direction in which the display unit 15 extends and the longitudinal direction of the porous sheet 10 is preferably a right angle (90 °). The width of the display unit 15 (when the angle between the extending direction of the display unit 15 and the longitudinal direction of the porous sheet 10 is a right angle) is preferably 2 to 8 mm, 3 to 5 mm is particularly preferable. There may be one display unit 15 or a plurality of display units 15. The number of display units 15 can be appropriately determined depending on the number of target nucleic acids to be captured. Further, the arrangement of the display unit 15 on the porous sheet 10 is not particularly limited, and can be appropriately determined depending on the type of the target nucleic acid.

  In the nucleic acid chromatography test tool 1 of the present embodiment, the concentration of the nucleic acid probe in the capture nucleic acid solid phase 16 is higher as it is closer to the display unit 15 in the thickness T direction. For this reason, the content of the nucleic acid probe at a deep position in the capture nucleic acid solid phase 16 is reduced, thereby reducing the content of the nucleic acid probe in the entire capture nucleic acid solid phase 16. Furthermore, even if the content of the nucleic acid probe in the capture nucleic acid solid phase 16 is small, the visibility of the display unit 15 that is the surface portion of the capture nucleic acid solid phase 16 is good. Even if the nucleic acid probe at a deep position of the capture nucleic acid solid phase 16 (position away from the display unit 15 in the “thickness direction of the porous sheet 10”) captures the target nucleic acid and develops color, the display unit 15 It cannot be seen. Therefore, the higher the concentration of the nucleic acid probe at the “shallow position of the capture nucleic acid solid phase 16” that can be visually recognized on the display unit 15, the higher the visibility of the display unit 15. Further, the smaller the number of nucleic acid probes in the deep position of the capture nucleic acid solid phase 16 (the position away from the display unit 15 in the “thickness direction of the porous sheet 10”), the more nucleic acid probes need not be used. Get better. Therefore, by increasing the concentration of the nucleic acid probe of the capture nucleic acid solid phase 16 in the thickness T direction as it is closer to the display unit 15, the visibility of the display unit 15 is improved while reducing the amount of nucleic acid probe used. It becomes possible.

  It can be confirmed as follows that “the closer the capture nucleic acid solid phase 16 is to the display unit 15 in the thickness T direction, the higher the concentration of the nucleic acid probe”. That is, the density of color of the supplemented nucleic acid solid phase cross section (enlarged 20 to 50 times) imaged with a microscope is compared with the color sample of each captured nucleic acid concentration to identify the concentration and confirm the concentration distribution. Then, from the concentration distribution obtained by such a method, it is confirmed that “the concentration of the nucleic acid probe is higher as the capture nucleic acid solid phase 16 is closer to the display unit 15 in the thickness T direction”. As the microscope, for example, “Nikon MEASURING MICROSCOPE MM-40” manufactured by Nikon Corporation can be used.

  The thickness T of the capture nucleic acid solid phase 16 may be less than 1 times the thickness t of the porous sheet 10. The thickness T is preferably 1/10 to 4/5 times the thickness t of the porous sheet 10, and more preferably 1/5 to 3/5 times. When the thickness T of the capture nucleic acid solid phase 16 is such a thickness, the content of the nucleic acid probe in the capture nucleic acid solid phase can be further reduced. If the thickness is the same as that of the porous sheet 10, a lot of nucleic acid probes may be used unnecessarily. Further, the thickness of the capture nucleic acid solid phase may be non-uniform. This is because the thickness may be non-uniform when the capture nucleic acid solution is allowed to penetrate into the porous substrate. The thickness T of the capture nucleic acid solid phase 16 when compared with the thickness t of the porous sheet 10 is the thickness of the thickest portion (deep portion) in the thickness of the non-uniform capture nucleic acid solid phase 16. That is.

  The shape of the capture nucleic acid solid phase 16 “cross section parallel to the“ longitudinal direction of the porous sheet 10 ”and perpendicular to the detection surface 13” (cross-sectional shape of the capture nucleic acid solid phase) is not particularly limited. The shape can be as follows. That is, as shown in FIG. 3, the cross-sectional shape of the capture nucleic acid solid phase is “a rectangle,“ a side that is parallel to the detection surface 13 and is located inside the porous sheet 10 ”is a curved shape that protrudes outward. The “formed shape” is preferred. In addition, the cross-sectional shape of the capture nucleic acid solid phase may be a rectangle.

  In the nucleic acid chromatography test tool 1 of the present embodiment, the porous sheet 10 preferably has a band-shaped position marker 14 (14a, 14b) extending perpendicularly to the longitudinal direction on the detection surface 13. The number of position markers is not particularly limited. The position marker is preferably arranged at a position on the outer side (side closer to the end) with respect to the display unit 15. The position marker 14 (14a, 14b) serves as an index for specifying the display unit 15 that is colored when the display unit 15 is colored. That is, the target nucleic acid corresponding to the colored display unit 15 can be specified by the positional relationship (for example, distance) between the colored display unit 15 and the position marker 14 (14a, 14b). The position marker 14 (14a, 14b) is preferably formed by applying a liquid containing pigment-based ink to the porous substrate 11 and drying it.

  As shown in FIG. 2, the nucleic acid chromatography test tool 1 of the present embodiment is a sheet-like backing member disposed on the other surface of the porous sheet 10 (the surface opposite to the detection surface 13). 20 is preferable. By providing the backing member 20, the shape of the nucleic acid chromatography test tool 1 can be easily maintained. A known backing member is used as the backing member 20, and for example, a laminated membrane card can be used. Further, as the laminated membrane card, those manufactured by Merck Millipore are preferably used.

  As shown in FIGS. 1 and 2, the nucleic acid chromatography test tool 1 of the present embodiment absorbs at the end (the other end) opposite to the sample collection unit 45 (the one end). A pad 40 is preferably provided. In the nucleic acid chromatography test tool 1 of the present embodiment, the absorption pad 40 is disposed on the surface of the porous sheet 10. The nucleic acid chromatography test tool 1 of the present embodiment has the absorption pad 40, so that the following effects can be obtained. That is, the sample collection part 45 of the nucleic acid chromatography test tool 1 is immersed in the sample, the sample is developed on the porous sheet 10, and the sample is made up to the “end opposite to the sample collection part 45” of the porous sheet 10. May expand the amount of sample absorbed from the sample collection unit 45. Therefore, when the absorption pad 40 is disposed at the “end opposite to the sample collection unit 45” of the nucleic acid chromatography test tool 1, the absorption pad 40 absorbs the sample, and thus absorbs from the sample collection unit 45. It is possible to suppress a decrease in the amount of sample (absorption rate of the sample).

  Examples of the material of the absorbent pad 40 include cellulose. The absorbent pad 40 is preferably porous so that the sample can be absorbed. Although the thickness of the absorption pad 40 is not specifically limited, 0.3-1.0 mm is preferable. The length of the absorbent pad 40 in the “longitudinal direction of the porous sheet” is not particularly limited, but is preferably 5 to 50 mm. The length of the absorbent pad 40 in the “direction perpendicular to the longitudinal direction of the porous sheet” is not particularly limited, but is preferably 2 to 8 mm. Moreover, it is also a preferable aspect that the length of the absorbent pad 40 in the “direction orthogonal to the longitudinal direction of the porous sheet” is the same as the length of the porous sheet in the “direction orthogonal to the longitudinal direction”.

(2) Manufacturing method of nucleic acid chromatography test tool:
One embodiment of the method for producing a nucleic acid chromatography test tool of the present invention includes a coating process and a drying process. The application step is a step in which the capture nucleic acid solution is applied in a band shape on one surface, which is the display portion forming surface of the sheet-like porous substrate that is long in one direction, and penetrates the porous substrate. The drying step is a step of drying the capture nucleic acid solution at 20 to 50 ° C. for 0.5 to 5 hours to form a capture nucleic acid solid phase. The capture nucleic acid solid phase is formed “so that a part is exposed” on the display portion forming surface. And the nucleic acid chromatography test | inspection tool of this invention mentioned above can be manufactured with the manufacturing method of the nucleic acid chromatography test | inspection tool of this embodiment. In addition, what provided the display part on the sheet-like porous base material (when it does not have a position marker), or what provided the display part and the position marker on the porous base material is a porous sheet. . Therefore, when a porous sheet does not have a position marker, a porous sheet can be obtained by forming a display part in a drying process. Further, when the porous sheet has a position marker, the porous sheet is formed by forming a “pre-drying position marker” on the porous substrate before the drying step, and forming the display portion and the position marker in the drying step. Can be obtained. When forming the “pre-drying position marker” on the porous substrate, it is preferable to apply a liquid containing pigment-based ink to the porous substrate. In addition, either the application of the capture nucleic acid solution or the application of a liquid containing pigment-based ink may be performed first.

  In the method for producing the nucleic acid chromatography test tool of the present embodiment, the capture nucleic acid solution applied to the porous substrate is dried at 20 to 50 ° C. for 0.5 to 5 hours in the drying step. At this time, most of the solvent of the capture nucleic acid solution evaporates from the application surface (the portion that becomes the display portion on the display portion forming surface). For this reason, during drying, the nucleic acid probe in the “capture nucleic acid solution that has entered the inside of the porous substrate” gradually moves toward the display portion forming surface (detection surface). Thereby, the thickness of the supplemented nucleic acid solid phase containing the nucleic acid probe is reduced compared to the thickness immediately after the capture nucleic acid solution is applied. Further, the nucleic acid probe gradually moves toward the display unit formation surface (detection surface), so that the concentration of the nucleic acid probe in the supplemental nucleic acid solid phase increases in the thickness direction as it approaches the display unit.

(2-1) Application process;
In the method for producing a nucleic acid chromatography test tool of the present embodiment, the coating step is to apply a capture nucleic acid solution in a band shape to the display portion forming surface of the sheet-like porous substrate, and display the capture nucleic acid solution containing the nucleic acid probe. This is a step of permeating into the porous base material from the part forming surface. In addition, the display part formation surface of a porous base material turns into a detection surface of a porous sheet. In addition, the conditions for the porous substrate are preferably the same as the conditions for the porous substrate that are preferable in the nucleic acid chromatography tester of the present invention described above.

  The application step is preferably a step of applying the capture nucleic acid solution in a band shape to the detection surface of the porous substrate with a micropipette 61 as shown in FIG. 4 and penetrating it into the porous sheet. Here, as shown in FIG. 4, the micropipette 61 has at least one base 63 formed with a cavity 62 filled with a capture nucleic acid solution, and at least one wall surface 64 of the base 63 forming the cavity 62. The piezoelectric / electrostrictive element 65 is provided. The micropipette 61 is configured such that the capture nucleic acid solution in the cavity 62 is discharged by the operation of the piezoelectric / electrostrictive element 65. In addition, the micropipette 61 is formed with an injection port 67 for injecting the capture nucleic acid solution into the cavity 62 and an ejection port 68 for discharging the capture nucleic acid solution injected into the cavity 62 to the outside. FIG. 4 is a schematic view showing a cross-section of a micropipette used in one embodiment of the method for producing a nucleic acid chromatography test tool of the present invention.

  The piezoelectric / electrostrictive element 65 is provided with electrode portions 66 on both sides. When a voltage is applied to the electrode portion 66, the piezoelectric / electrostrictive element 65 vibrates (deforms) in the vibration direction X indicated by “open arrow”. As a result, the vibrating portion 69, which is “one wall of the base 63” where the piezoelectric / electrostrictive element 65 is disposed, vibrates (deforms), and the volume of the cavity 62 changes. By this operation (vibration) (by repeating the deformation of the vibration unit 69), the capture nucleic acid solution is injected into the cavity 62 through the injection port 67, and the injected capture nucleic acid solution is discharged through the discharge port 68. In FIG. 4, the flow Y of the capture nucleic acid solution is indicated by an arrow.

  The discharge amount of the capture nucleic acid solution by one discharge operation (one deformation of the vibrating portion) of the micropipette 61 is preferably 0.02 to 0.4 μl (microliter). In this way, a desired thin capture nucleic acid solid phase can be formed by discharging the capture nucleic acid solution little by little with the micropipette 61 and applying the capture nucleic acid solution to the display portion forming surface of the porous substrate. It becomes easier. When the discharge amount of the capture nucleic acid solution by the single discharge operation of the micropipette 61 is less than 0.02 μl (microliter), the number of discharges for discharging the required amount of the capture nucleic acid solution increases and the application time is long. May be. When the amount is larger than 0.4 μl (microliter), the thickness of the capture nucleic acid solid phase increases, and many nucleic acid probes may be used in vain.

  The material of the piezoelectric / electrostrictive element 65 is: lead zirconate, lead titanate, lead magnesium niobate, lead magnesium tantalate, lead nickel niobate, lead zinc niobate, lead manganese niobate, lead antimony stannate, manganese A ceramic containing at least one selected from the group consisting of lead tungstate, lead cobalt niobate and barium titanate is preferable.

  The material of the base 63 of the micropipette 61 is preferably stabilized zirconia, partially stabilized zirconia, alumina, magnesia, silicon nitride, or the like.

  Furthermore, it is preferable to apply the capture nucleic acid solution using a dispensing device 71 formed by arranging a plurality of micropipettes 61 on a fixed plate 72 as shown in FIG. Thereby, the capture nucleic acid solution can be efficiently applied to the display portion forming surface of the porous substrate. The material of the fixing plate 72 is not particularly limited, and may be a material that is preferable as the material of the base 63 of the micropipette 61, for example. FIG. 5 is a schematic view showing a cross section of a dispensing device 71 formed by arranging a plurality of micropipettes 61 used in an embodiment of the method for producing a nucleic acid chromatography test tool of the present invention.

  The number and arrangement of the micropipettes 61 provided in the dispensing device 71 are not particularly limited, and can be appropriately determined according to the nucleic acid chromatography test tool to be manufactured.

(2-2) “Pre-drying position marker” forming step;
When preparing a nucleic acid chromatography test tool having a position marker, it is preferable to form a “position marker before drying” on the display portion forming surface of the sheet-like porous substrate before drying. The “position marker before drying” is the “position marker” after drying in the drying process.

  The “pre-drying position marker” is formed by applying “a liquid containing pigment-based ink” or the like in the “method of applying the capture nucleic acid solution in a strip shape to the display surface forming surface of the porous substrate” in the application step. It is preferable that it is a method to do. The pre-drying position marker is preferably a porous substrate formed by impregnating a liquid containing pigment-based ink or the like (portion impregnated with a liquid containing pigment-based ink).

(2-3) drying step;
In the method for producing a nucleic acid chromatography test tool of the present embodiment, the drying step is to dry the capture nucleic acid solution at 20 to 50 ° C. for 0.5 to 5 hours. As described above is the step of forming a capture nucleic acid solid phase. When the position marker is not formed, a porous sheet is formed thereby. Further, when the backing member and the absorption pad are not formed, a nucleic acid chromatography test tool can be obtained thereby.

  As described above, in the method for producing a nucleic acid chromatography test tool of this embodiment, it is preferable to dry the capture nucleic acid solution applied to the porous substrate at a low temperature by extending the drying time. Thereby, the capture nucleic acid solution applied to the porous substrate can be moved to the display portion forming surface side of the porous substrate. Then, by moving the capture nucleic acid solution (nucleic acid probe) to the display portion forming surface side of the porous substrate, the thickness of the supplemental nucleic acid solid phase can be reduced. Furthermore, the above drying conditions (20 to 50 ° C., drying for 0.5 to 5 hours) allow sufficient time for the capture nucleic acid solution to move to the “display surface of the porous substrate” side. At the same time, the manufacturing time can be prevented from becoming too long. Furthermore, since the capture nucleic acid solution (nucleic acid probe) can be moved gradually toward the display portion forming surface (detection surface) side under the above drying conditions, the concentration of the nucleic acid probe in the capture nucleic acid solid phase can be adjusted in the thickness direction. The closer to the display, the darker it can be.

  The drying temperature is preferably 20 to 50 ° C. When the drying temperature is lower than 20 ° C., it is not preferable because drying of the capture nucleic acid solution takes a long time and the manufacturing time becomes long. A drying temperature higher than 50 ° C. is not preferable because a solid phase is formed before the concentration distribution is formed. The drying time is preferably 0.5 to 5 hours. A drying time shorter than 0.5 hour is not preferable because the capture nucleic acid solution is not sufficiently dried. If the drying time is longer than 5 hours, the production time becomes longer, which is not preferable.

  The drying method is not particularly limited, and examples thereof include a drying method using a thermostat.

(2-4) backing member forming step;
It is preferable to attach a sheet-like backing member to the “surface opposite to the detection surface 13” of the porous sheet. Each condition for the backing member is preferably the same as the preferable condition for the backing member provided in the nucleic acid chromatography test instrument of the present invention. In the case where an absorbent pad is not formed, a nucleic acid chromatography test tool is obtained thereby.

(2-5) absorption pad forming step;
It is preferable to obtain a nucleic acid chromatography test tool by attaching an absorption pad to the surface of the porous sheet at the end (the other end) opposite to the sample collection part (the one end). Each condition for the absorbent pad is preferably the same as the preferred condition for the absorbent pad provided in the nucleic acid chromatography test instrument of the present invention.

  EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples.

Example 1
The capture nucleic acid solution was applied in a band shape to one surface, which is the display portion forming surface of the sheet-like porous substrate that is long in one direction.

  As the porous substrate, “Hi-Flow Plus membrane sheet HF180 4 mil Mylar backing” (30.5 cm × 25 cm) manufactured by Merck Millipore was used. The porous substrate is composed of two layers: a nitrocellulose layer with a thickness of 135 μm (± 19 μm) and a Mylar backing (PET) layer with a thickness of 100 μm. The surface of the nitrocellulose layer is formed on the display part. The surface.

  Two types were prepared by dissolving a synthetic oligo DNA as a nucleic acid probe in “Tris-EDTA buffer” as a solvent, SSC buffer solution (Saline-sodium citrate solution) and bromophenol blue. The obtained ones are capture nucleic acid solutions A and B, which are different in type of synthetic oligo DNA. The synthetic oligo DNA in the capture nucleic acid solution A had the sequence “GTCCGAAAATTACAACGAAGCGAA”, and the synthetic oligo DNA in the capture nucleic acid solution B had the sequence “TGCTTCTACGAGTTGATAGGTGTA”.

  The capture nucleic acid solution was applied to the porous substrate by a method using a micropipette (GENESHOT (registered trademark) spot method using a GENESHOT (registered trademark) spotter). The GENESHOT (registered trademark) spotter is an apparatus using a discharge unit (inkjet method) described in Japanese Patent Application Laid-Open No. 2003-75305. The application frequency of the capture nucleic acid solutions A and B (application amount per unit length (cm) in the “length direction of the applied part”) was 0.15 μl (microliter) / cm, respectively. The concentrations of the capture nucleic acid solutions A and B were “6” when the standard of the concentration of the capture nucleic acid solution was “1”. In addition, the density | concentration of the capture nucleic acid solution in the comparative example 2 mentioned later was made into the standard ("1") of the density | concentration of a capture nucleic acid solution.

  The capture nucleic acid solution A is applied at a position of 16 mm and the capture nucleic acid solution B is applied at a position of 19 mm from one end (sample collection part) of the porous substrate on the display portion forming surface of the porous substrate. did. The capture nucleic acid solution was applied so as to extend in a direction perpendicular to the longitudinal direction of the porous substrate. The length of the capture nucleic acid solution (the length of the coated portion in the length direction) was the same as the width of the porous substrate (3.5 mm). The length direction of the “applied portion” of the capture nucleic acid solution was the width direction of the porous substrate. The width of the capture nucleic acid solution (the length in the “longitudinal direction of the porous substrate”) was 0.5 mm.

  Next, pigment-based magenta ink was applied to the porous substrate to form a pre-drying position marker. For the application of the pigment-based magenta ink, the above-described micropipette method (GENESHOT (registered trademark) spot method) was used.

  Next, the porous substrate coated with the capture nucleic acid solution and the pigment-based magenta ink was dried using a thermostat (DK600 manufactured by Yamato Scientific Co., Ltd.) to obtain a porous sheet. Drying conditions were 50 ° C. and 0.5 hour. A capture nucleic acid solid phase A was formed by the capture nucleic acid solution A, and a capture nucleic acid solid phase B was formed by the capture nucleic acid solution B.

  Next, a backing member was disposed on the surface of the obtained porous sheet opposite to the detection surface. As the backing member, “Laminated membrane card” (60 mm × 300 mm) manufactured by Merck Millipore was used.

  Next, “Merck Millipore's absorbent pad (20 mm × 300 mm)” is attached to the porous sheet using an adhesive tape (25 mm × 300 mm) so as to cover about 5 mm as an adhesive surface, and a composite sheet is obtained. It was. About the obtained composite sheet, it cut | disconnected using the cutting machine and obtained 85 nucleic acid chromatography test | inspection tools of width 3.5mm and length 60mm.

  In the obtained nucleic acid chromatography test tool, the thickness of the capture nucleic acid solid phase was ½ times the thickness of the porous sheet. The thickness of the capture nucleic acid solid phase is a value obtained by taking a microscope image and measuring the thickness using “Nikon MEASURING MICROSCOPE MM-40” manufactured by Nikon.

  Further, in the obtained nucleic acid chromatography test tool, the concentration of the nucleic acid probe in the thickness direction of the capture nucleic acid solid phase was higher as it was closer to the detection surface (display portion). The concentration (concentration distribution) of the nucleic acid probe in the thickness direction of the capture nucleic acid solid phase is compared with the color density of the cross-section of the complementary nucleic acid solid phase (enlarged by 20 times) imaged with a microscope and the color sample for each capture nucleic acid concentration. And determined by specifying the concentration. As the microscope, “Nikon MEASURING MICROSCOPE MM-40” manufactured by Nikon was used.

  Using the obtained nucleic acid chromatography test tool, “Target Nucleic Acid Detection Test 1” and “Target Nucleic Acid Detection Test 2” were performed by the method shown below. The results are shown in Table 1.

  In Table 1, the column “Capture Nucleic Acid” shows the concentration of the capture nucleic acid solutions A and B (standard is 1) and the coating density (note that the concentrations of the capture nucleic acid solutions A and B are the same). .) The column “Thickness of capture nucleic acid solid phase” indicates how many times the thickness of the capture nucleic acid solid phase is greater than the thickness of the porous sheet. The column of “Nucleic acid probe concentration” shows the concentration distribution of the nucleic acid probe in the capture nucleic acid solid phases A and B (the concentration distribution of the capture nucleic acid solid phases A and B was the same). In the column of “Nucleic acid probe concentration”, “A” indicates that the concentration of the nucleic acid probe in the capture nucleic acid solid phase is higher as it is closer to the display portion, and “B” indicates that the concentration in the capture nucleic acid solid phase is higher. This indicates that the closer the concentration of the nucleic acid probe is to the display portion, the higher the state is.

(Target nucleic acid detection test 1)
“Sample DNA having a base sequence complementary to the base sequence of the capture nucleic acid solution A (modified with an amino group)” is immobilized on carboxylated latex particles. The carboxylated latex particles on which the sample DNA is immobilized are dispersed with sterilized water to obtain a sample (sample A). Sample A is put in a test tube, and the sample collection part of the nucleic acid chromatography test tool is immersed in sample A in the test tube. After 5 minutes, observe the detection surface of the nucleic acid chromatography test tool. The evaluation results are “C” when the color is confirmed, “B” when the clear color is confirmed, and “A” when the color darker than “B” is confirmed. Assess the evaluation result “A”.

(Target nucleic acid detection test 2)
"Sample DNA having a base sequence complementary to the base sequence of the capture nucleic acid solution A (modified with an amino group)" and "Sample DNA having a base sequence complementary to the base sequence of the capture nucleic acid solution B (note that amino Is modified) on the carboxylated latex particles. The carboxylated latex particles on which the sample DNA is immobilized are dispersed with sterilized water to obtain a sample (sample B). Sample B is put in a test tube, and the sample collection part of the nucleic acid chromatography test tool is immersed in sample B in the test tube. The time (color development time interval) from when the capture nucleic acid solid phase A is colored until the capture nucleic acid solid phase B is colored is "A" for less than 1 minute, "B" for less than 1 to 3 minutes, 3 minutes The above is referred to as “C”. Further, after 5 minutes, the detection surface of the captured nucleic acid solid phase B of the nucleic acid chromatography test tool is observed. As an evaluation result (color development evaluation of the capture nucleic acid solid phase B), “C” was confirmed when color development was confirmed, and “B” and color development darker than “B” were confirmed when clear color development was confirmed. The case is “A”. Assess the evaluation result “A”.

(Example 2, Comparative Examples 1-3)
A nucleic acid chromatography test tool was prepared in the same manner as in Example 1 except that the conditions were as shown in Table 1. In the same manner as in Example 1, target nucleic acid detection test 1 and target nucleic acid detection test 2 were performed. The results are shown in Table 1. In addition, Table 1 shows “the thickness of the capture nucleic acid solid phase” and “the concentration of the nucleic acid probe”. Further, in the nucleic acid chromatography test tools of Examples 1 and 2 and Comparative Example 1, the total coating amount for each of the capture nucleic acid solutions A and B was the same amount. In the nucleic acid chromatography test tool of Comparative Example 2, the total coating amount for each of the capture nucleic acid solutions A and B was the same as the coating amount in Examples 1 and 2 and Comparative Example 1.

  From Table 1, since the nucleic acid chromatography test tools of Examples 1 and 2 were dried at a low temperature for a long time, "the state where the concentration of the nucleic acid probe in the capture nucleic acid solid phase was closer to the display portion was higher" there were. On the other hand, since the nucleic acid chromatography test tool of Comparative Example 1 was dried at a high temperature for a short time, it was not “a state where the concentration of the nucleic acid probe in the capture nucleic acid solid phase was higher as it was closer to the display portion”. In addition, the nucleic acid chromatography test tool of Comparative Example 2 has the same total amount of capture nucleic acid as that of Examples 1 and 2, but was dried at a high temperature for a short time, so that “the concentration of the nucleic acid probe in the solid phase of the capture nucleic acid” Was not so high that it was closer to the display. In the nucleic acid chromatography test tool of Comparative Example 3, the coating concentration was the same as in Examples 1 and 2, and the coating frequency was 1.00 μl / cm and a large amount of capture nucleic acid was added. However, since the drying was performed at a high temperature for a short time, it was not “a state where the concentration of the nucleic acid probe in the capture nucleic acid solid phase was higher as it was closer to the display portion”.

  Moreover, it can be seen from the results of the target nucleic acid detection test 1 in Table 1 that the nucleic acid chromatography test tools of Examples 1 and 2 have a deep color in the display part and are excellent in the visibility of the display part. This is because “the concentration of the nucleic acid probe in the solid phase of the capture nucleic acid is higher as it is closer to the display portion”. The nucleic acid chromatography test tool of Comparative Example 1 was confirmed to have a clear color, but not a sufficient color. This is because it was not “a state where the concentration of the nucleic acid probe in the capture nucleic acid solid phase was higher as it was closer to the display portion”. In the nucleic acid chromatography test tool of Comparative Example 2, since the coating concentration of the capture nucleic acid was low, color development was confirmed, but the color development was not sufficient. Although the nucleic acid chromatography test tool of Comparative Example 3 was confirmed to develop color, it was inferior to Examples 1 and 2. This is because it was not “a state where the concentration of the nucleic acid probe in the capture nucleic acid solid phase was higher as it was closer to the display portion”.

  In addition, from the results of the target nucleic acid detection test 2 in Table 1, the nucleic acid chromatography test tool of Examples 1 and 2 developed a fast color display when detecting a plurality of nucleic acids, and the display unit (particularly the captured nucleic acid fixation). It can be seen that the visibility of the phase B display portion) is excellent. Since the nucleic acid chromatography test tool of Comparative Example 1 was dried at a high temperature for a short time, it was not “a state where the concentration of the nucleic acid probe in the capture nucleic acid solid phase was lowered at a deep position”. It became long. In addition, although clear color development was confirmed for the capture nucleic acid solid phase B, it was not sufficient color development. Since the nucleic acid chromatography test tool of Comparative Example 2 was not “in a state where the concentration of the nucleic acid probe in the capture nucleic acid solid phase was lowered at a deep position”, the color development time interval became very long. Furthermore, since the coating concentration of the capture nucleic acid solution was low, color development was confirmed for the capture nucleic acid solid phase B, but the color development was not sufficient. As described above, the nucleic acid chromatography test tool of Comparative Example 3 had the same coating concentration as in Examples 1 and 2 and a coating frequency of 1.00 μl / cm and a large amount of capture nucleic acid was added. However, since drying was performed at high temperature for a short time, it was not “a state where the concentration of the nucleic acid probe in the capture nucleic acid solid phase was lowered at a deep position”. Therefore, the color development time interval became very long. Furthermore, since the nucleic acid chromatography test tool of Comparative Example 3 was not in a “state where the concentration of the nucleic acid probe in the capture nucleic acid solid phase was lowered at a deep position”, color development on the capture nucleic acid solid phase B was confirmed. The results were inferior to those of Examples 1 and 2.

  The nucleic acid chromatography test tool of the present invention can be used for diagnosis related to infectious diseases and allergies, and detection of bacteria and viruses. Moreover, the method for producing a nucleic acid chromatography test tool of the present invention can be used for the production of such a nucleic acid chromatography test tool of the present invention.

1: Nucleic acid chromatography test tool, 10: Porous sheet, 11: Porous base material, 12: Display part forming surface, 13: Detection surface, 14, 14a, 14b: Position marker, 15, 15a, 15b: Display part 16, 16a, 16b: capture nucleic acid solid phase, 20: backing member, 40: absorption pad, 45: sample collection part, 61: micropipette, 62: cavity, 63: substrate, 64: wall surface, 65: piezoelectric / electric Strain element, 66: electrode part, 67: injection port, 68: discharge port, 69: vibration part, 71: dispensing device, 72: fixing plate, X: vibration direction, Y: flow of capture nucleic acid solution, T: capture Nucleic acid solid phase thickness, t: porous sheet thickness.

Claims (7)

A porous sheet that is long in one direction that can develop a sample containing the target nucleic acid,
The porous sheet has a sheet-like porous substrate having one surface as a display portion forming surface,
The porous substrate has a capture nucleic acid solid phase formed so that a nucleic acid probe for capturing a target nucleic acid permeates from the display part formation surface and a part is exposed on the display part formation surface,
The portion of the capture nucleic acid solid phase exposed on the display portion forming surface of the porous substrate is a display portion,
The display unit is a strip extending at an angle intersecting the longitudinal direction of the porous sheet,
The nucleic acid chromatography test tool, wherein the concentration of the nucleic acid probe is higher as the capture nucleic acid solid phase is closer to the display unit in the thickness direction.   The nucleic acid chromatography test tool according to claim 1, wherein the thickness of the capture nucleic acid solid phase is non-uniform and less than 1 times the thickness of the porous sheet.   The nucleic acid chromatography test tool according to claim 1, further comprising a sheet-like backing member disposed on the other surface of the porous substrate.   The nucleic acid chromatography test instrument according to any one of claims 1 to 3, wherein the porous sheet is a multi-layer sheet. A method for producing a nucleic acid chromatography test instrument according to any one of claims 1 to 4,
On one side the A display unit forming surface of a long sheet of the porous substrate in one direction, is applied to the strip to penetrate into the porous substrate capture nucleic acid solution by a plurality of ejection of micropipette Application process;
The capture nucleic acid solution, at 20 to 50 ° C., dried 0.5-5 hours, the production method of the nucleic acid chromatographic test device having a drying step of forming the capture nucleic acid solid phase. 6. The method for producing a nucleic acid chromatography test instrument according to claim 5, wherein a discharge amount of the capture nucleic acid solution by one discharge operation of the micropipette is 0.02 to 0.4 [mu] l. The coating step includes one or more substrates on which cavities filled with the capture nucleic acid solution are formed, and piezoelectric / electrostrictive elements disposed on at least one wall surface of the substrate forming the cavities. , coating said by configured the micropipette such that the capture nucleic acid solution in the cavity is discharged by operation of the piezoelectric / electrostrictive element, said capture nucleic acid solution to strip the detection surface of the porous substrate The method for producing a nucleic acid chromatography test tool according to claim 5 or 6 , wherein the method is a step of permeating into the porous substrate.

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