JP5971597B2 - Mushroom bed cultivation bag outer needle hole inoculation method - Google Patents
Mushroom bed cultivation bag outer needle hole inoculation method Download PDFInfo
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- JP5971597B2 JP5971597B2 JP2013168367A JP2013168367A JP5971597B2 JP 5971597 B2 JP5971597 B2 JP 5971597B2 JP 2013168367 A JP2013168367 A JP 2013168367A JP 2013168367 A JP2013168367 A JP 2013168367A JP 5971597 B2 JP5971597 B2 JP 5971597B2
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この発明は、きのこ菌床栽培でのオガ種菌の接種方法に関するものであり殺菌培地袋外に取り付けたオガ種菌上より針で殺菌培地袋に穴を開けて行なう接種方法のことである。 The present invention relates to a method for inoculating Oga inoculum in mushroom fungus bed cultivation, and is an inoculation method in which a hole is made in a sterilization medium bag from above the Oga inoculum attached outside the sterilization medium bag.
現在、きのこ菌床栽培でのオガ種菌接種までの行程では、培地を栽培袋に詰め袋口を仮封して、高温殺菌して冷却してから仮封した袋口を開き袋内の殺菌培地上にオガ種菌を接種している、このため、培地を殺菌後、冷却及び接種時、殺菌培地が外気にふれるきかいも多く、雑菌混入を防ぐため、設備費のかかるクリーンな接種室を必要とし、接種室の消毒に薬剤も多く使用している。 Currently, in the process up to the inoculation with mushroom fungus bed cultivation, the culture medium is packed in a cultivation bag, the bag mouth is temporarily sealed, the pasteurized bag mouth is opened after being sterilized by high temperature sterilization, and the bag is sterilized. Oga seeds are inoculated on the top. Therefore, after sterilizing the medium, the sterilizing medium is exposed to the outside air at the time of cooling and inoculation, so a clean inoculation room that requires equipment costs is necessary to prevent contamination with germs. And many drugs are used to disinfect the inoculation room.
きのこ菌床袋栽培での菌床製造行程での殺菌培地袋内にオガ種菌を直接、接種する方法には、次の様な欠点があった。接種時に袋口を開くため、殺菌前に、培地袋口を完全に封することができず、仮封の手間と、高温殺菌してから冷却までと、接種時に袋口より殺菌培地が外気にふれるきかいが多く、雑菌混入を防ぐため、設備費のかかるクリーンな接種室を必要とし接種室の消毒に薬剤も多く使用している。本発明は以上のような欠点をなくすためになされたものである。 The method of directly inoculating Oga seeds in a sterilized medium bag during the fungus bed production process in mushroom fungus bed cultivation has the following drawbacks. Since the bag mouth is opened at the time of inoculation, the medium bag mouth cannot be completely sealed before sterilization. In order to prevent contamination by many bacteria, a clean inoculation room that requires equipment costs is required, and a lot of drugs are used to disinfect the inoculation room. The present invention has been made to eliminate the above drawbacks.
殺菌培地袋(3)外に、オガ種菌を個包装(1)して取り付け、その上より、清潔な針(4)で、殺菌培地袋(3)に、外部より穴を開けて接種する方法により、仮封の手間と、冷却時及び、接種時の外気による雑菌混入の心配も少ない、きのこ菌床栽培袋外針穴接種法で解決できる。 A method of inoculating outside the sterilization medium bag (3) with a clean needle (4) and opening a hole in the sterilization medium bag (3) from outside. Therefore, it can be solved by the mushroom fungus bed cultivation bag outer needle hole inoculation method, which is less troublesome for temporary sealing and less worrying about contamination by outside air during cooling and inoculation.
本発明は、殺菌培地袋外より接種するため、殺菌前に袋口を完全に封することができ、仮封の手間が省け、冷却時と接種時も、殺菌培地が外気にふれることがほとんどないため、設備費のかかるクリーンな接種室でなくても、簡易な場所でも外気による雑菌混入の心配もなくおこなえ、接種室消毒の薬剤も減らすことができる。殺菌培地と袋の密着面であれば細長い菌床、平たく大きな菌床等、形、大きさ、接種位置を気にせず復数場所でも接種でき、培養期間も短縮可能であり、きのこ栽培上有利になる。 Since the present invention is inoculated from outside the sterilization medium bag, the bag mouth can be completely sealed before sterilization, the need for temporary sealing can be saved, and the sterilization medium can be exposed to the outside air during cooling and inoculation. Therefore, even if it is not a clean inoculation room that requires equipment costs, it can be performed in a simple place without worrying about contamination by outside air, and the disinfecting agent for inoculation room can be reduced. As long as the sterilization medium and the bag are in close contact with each other, it can be inoculated in repetitive places without worrying about the shape, size, inoculation position, etc., and the cultivation period can be shortened. become.
以下、本発明を実施するための形態について説明する。
殺菌培地袋(3)外に、オガ種菌を直接、粘着テープ等で覆いかぶせて取りつけてもよいが、作業効率を孝え、先に、オガ種菌を4cm3程度に個包装(1)して、両面テープか接着剤等で取り付け、その上より清潔な針(4)で殺菌培地袋(3)に穴を開ける、針を抜く時、外気が入らないように指で押さえておくと良い、殺菌培地(2)が外気にふれることもほとんどなく接種でき0.数mmの穴より菌糸がのび殺菌培地(2)に活着する。
本発明は、以上のような接種方法で行なうことである。Hereinafter, modes for carrying out the present invention will be described.
Oga inoculum may be directly covered with adhesive tape, etc. outside the sterilization medium bag (3), but the working efficiency is enhanced, and the Oga inoculum is first wrapped (about 4 cm 3 ). , Attach with double-sided tape or adhesive, etc., and make a hole in the sterilization medium bag (3) with a clean needle (4), and hold it with your fingers so that outside air does not enter when removing the needle, Sterilization medium (2) can be inoculated with little contact with outside air. The mycelium grows from the hole of several mm and settles on the sterilization medium (2).
The present invention is performed by the inoculation method as described above.
1 個包装したオガ菌
2 殺菌培地
3 殺菌培地袋
4 針1 packaged Oga bacteria 2
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