JP5748947B2 - 2-Acylaminothiazole derivative and method for producing the same - Google Patents

Description

  The present invention relates to a thiazole derivative that can be used for analysis or purification of proteins, particularly immunoglobulins, and a method for producing the same.

  In recent years, the demand for medical proteins is expanding, and in particular, establishment of an industrial purification technique that can be carried out easily and on a large scale for immunoglobulins is eagerly desired. Generally, immunoglobulin purification techniques include chromatographic separation techniques such as gel filtration chromatography, ion exchange chromatography, hydrophobic interaction chromatography, reverse phase chromatography, and affinity chromatography. Is industrially implemented, it is difficult to say that the use of a separating agent or apparatus used in a laboratory is not economical and practical. In addition, since many kinds of proteins are mixed in the process of industrially producing immunoglobulins, it is extremely difficult to obtain the target immunoglobulin purely using one of the purification techniques described above. . For this reason, a purification process in which several techniques are combined is usually employed.

  By the way, like the thiazole derivative (1) of the present invention, a hydroxyalkyl group, a formylalkyl group, a mercaptoalkyl group, a carboxyalkyl group, and an aminoxyalkyl group are present at the 5-position of the thiazole ring, and the arylcarbonylamino group at the 2-position There is no report on thiazole derivatives having a group. In recent years, many low molecular weight compounds for purifying immunoglobulins and matrices to which they are bound have been disclosed, but the thiazole derivative (1) of the present invention is useful as a low molecular weight compound for purifying immunoglobulins. There is no report. Although a thiazole derivative having a chemical structure similar to the thiazole derivative (1) of the present invention is reported in Non-Patent Document 1, the thiazole derivative described in this document has a chemical structure in which the 2-position of the thiazole ring is an amino group or There is no description of compounds limited to alkyl groups and having an arylcarbonylamino group at the 2-position as in the thiazole derivative (1) of the present invention, and these compounds are used for affinity analysis for analysis or purification of useful proteins. There is no description that it can be used as a gel. Non-Patent Documents 2 to 8 and Patent Document 1 disclose compounds having a hydroxyalkyl group or a carboxyalkyl group at the 5-position of the thiazole ring, but the thiazole ring such as the thiazole derivative (1) of the present invention is disclosed. There is no description regarding a compound having an arylcarbonylamino group at the 2-position, and there is no description as an affinity gel for analysis or purification of useful proteins.

Japanese Patent No. 384496

Journal of Medicinal Chemistry, 35 (17), 3239-3246, 1992 Journal future Praktice Chemie, 314 (5-6), 961-964, 1972 Journal of Indian Chemical Society, 15, 160, 1938 Chemical Abstracts, 35, 5109, 1941 Berichte, 29, 1042, 1896 Helvetica Chimica Acta, 28, 362, 1945 Farmaco, Editione Scientific, 41 (6), 483-498, 1986. Annalen der Chemie, Justus Liebigs, 596, 80-158, 1955 First Ligand Coupling Handbook, Amersham Biosciences, 2005

  The problem to be solved by the present invention is to provide a thiazole derivative having a strong affinity for proteins, particularly immunoglobulins, and useful for analysis or purification of immunoglobulins, and a method for producing the same.

  As a result of intensive studies to solve the above-mentioned problems, the inventors of the present invention have an arylcarbonylamino group at the 2-position, a hydroxyalkyl group, a formylalkyl group, a mercaptoalkyl group, a carboxyalkyl group, an aminoxyalkyl at the 5-position. The present inventors have found that the thiazole derivative represented by the general formula (1) into which a group is introduced has a strong affinity for proteins, particularly immunoglobulins, and has completed the present invention.

  That is, the present invention relates to the general formula (1)

（式中、Ｒ^１及びＲ^２は水素原子又は置換されていてもよい炭素数１から６のアルキル基を表し、Ｒ^３はヒドロキシ基、ホルミル基、メルカプト基、カルボキシル基、アミノキシ基（−ＯＮＨ_２）、アセトキシ基、又は炭素数１から６のアルコキシチオカルボニルチオ基を表し、Ａｒは置換されていてもよい芳香族基を表し、ｎは１から１２の整数を表す。）で表されるチアゾール誘導体に関するものである。

(In the formula, R ¹ and R ² represent a hydrogen atom or an optionally substituted alkyl group having 1 to 6 carbon atoms, and R ³ represents a hydroxy group, a formyl group, a mercapto group, a carboxyl group, an aminoxy group (—ONH ₂ ), an acetoxy group, or an alkoxythiocarbonylthio group having 1 to 6 carbon atoms, Ar represents an optionally substituted aromatic group, and n represents an integer of 1 to 12. It relates to a thiazole derivative.

  The present invention also provides a general formula (2)

（式中、Ｒ^１及びｎは前記と同じ意味を表し、Ｒ^３ａはフタルイミドオキシ基、アセトキシ基、カルボキシル基又は炭素数１から６のアルコキシチオカルボニルチオ基を表し、Ｙはハロゲン原子を表す。）で表されるα−ハロケトン体と、一般式（３）

(Wherein R ¹ and n represent the same meaning as described above, R ^3a represents a phthalimidooxy group, an acetoxy group, a carboxyl group, or an alkoxythiocarbonylthio group having 1 to 6 carbon atoms, and Y represents a halogen atom. And an α-haloketone body represented by the general formula (3)

（式中、Ｒ^２及びＡｒは前記と同じ意味を表す。）で表されるアシルチオウレア誘導体を反応させ、一般式（１ａ）

(In the formula, R ² and Ar have the same meanings as described above), and an acylthiourea derivative represented by the general formula (1a) is reacted.

（式中、Ｒ^１、Ｒ^２、Ｒ^３ａ、Ａｒ及びｎは前記と同じ意味を表す。）で示されるチアゾール誘導体を製造する方法に関するものである。

(Wherein R ¹ , R ² , R ^3a , Ar and n represent the same meaning as described above).

  Moreover, the present invention provides a compound represented by the general formula (1aa)

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）で表されるチアゾール誘導体を水存在下にてヘキサカルボニルモリブテンと反応させた後に脱保護して、一般式（１ａｂ）

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above), the thiazole derivative represented by the above formula is reacted with hexacarbonylmolybten in the presence of water and then deprotected to give a general formula ( 1ab)

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）で示されるチアゾール誘導体を製造する方法に関するものである。

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above).

  The present invention also provides a compound of the general formula (1ac)

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表し、Ｒ^４は炭素数１から６のアルキル基を表す。）を塩基性条件下にて反応させ、一般式（１ａｄ）

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above, and R ⁴ represents an alkyl group having 1 to 6 carbon atoms) are reacted under basic conditions to give a general formula (1ad )

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）で示されるチアゾール誘導体を製造する方法に関するものである。

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above).

  The present invention also provides a compound of the general formula (1ab)

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）で表されるチアゾール誘導体を酸化させ、一般式（１ａｅ）

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above), and the thiazole derivative represented by the general formula (1ae)

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）で示されるチアゾール誘導体を製造する方法に関するものである。

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above).

  The present invention also provides a general formula (2)

（式中、Ｒ^１、Ｒ^３ａ、Ｙ及びｎは前記と同じ意味を表す。）で表されるα−ハロケトン体と、チオウレア（３ａ）

(Wherein R ¹ , R ^3a , Y and n represent the same meaning as described above) and a thiourea (3a)

を反応させ、一般式（１ｂ）

To give a general formula (1b)

（式中、Ｒ^１、Ｒ^３ａ及びｎは前記と同じ意味を表す。）で示されるチアゾール誘導体を製造する方法に関するものである。

(Wherein R ¹ , R ^3a and n represent the same meanings as described above).

  The present invention also provides a compound represented by the general formula (1b)

（式中、Ｒ^１、Ｒ^３ａ及びｎは前記と同じ意味を表す。）で示されるチアゾール誘導体と、一般式（４）

(Wherein R ¹ , R ^3a and n represent the same meaning as described above) and a general formula (4)

（式中、Ａｒは前記と同じ意味を表し、Ｚはハロゲン原子を表す。）で表される酸ハロゲン化物を反応させ、一般式（１ａｆ）

(Wherein Ar represents the same meaning as described above, and Z represents a halogen atom) is reacted with an acid halide represented by the general formula (1af)

（式中、Ｒ^１、Ｒ^３ａ、Ａｒ及びｎは前記と同じ意味を表す。）で示されるチアゾール誘導体を製造する方法に関するものである。

(Wherein R ¹ , R ^3a , Ar and n represent the same meaning as described above).

  Moreover, the present invention provides a compound represented by the general formula (1aa)

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）で示されるチアゾール誘導体（１ａａ）をアミン類と反応させ、一般式（１ａａａ）

(Wherein R ¹ , R ² , Ar and n represent the same meanings as described above)

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）で示されるチアゾール誘導体を製造する方法に関するものである。

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above).

  Hereinafter, the present invention will be described in more detail.

Definitions of substituents represented by R ¹ , R ² , R ³ , R ^3a , R ⁴ , Ar, Y and Z in the thiazole derivative of the present invention are shown below.

The optionally substituted alkyl group represented by R ¹ and R ² may be linear or branched, and may be a methyl group, an ethyl group, a propyl group, Isopropyl group, butyl group, isobutyl group, sec-butyl group, tert-butyl group, pentyl group, isoamyl group, neopentyl group, 2-pentyl group, 3-pentyl group, tert-pentyl group, hexyl group, isohexyl group, 2 -A hexyl group and a 3-hexyl group can be illustrated. Further, these alkyl groups having 1 to 6 carbon atoms may be substituted with a halogen atom or the like, and more specifically, a difluoromethyl group, a trifluoromethyl group, a 2,2,2-trifluoroethyl group, a per Examples thereof include a fluorobutyl group, a hydroxymethyl group, a carboxymethyl group, a methylthiomethyl group, and a 4-morpholinocarbonylmethyl group. R ¹ is preferably a methyl group, and R ² is preferably a hydrogen atom from the viewpoint of showing good performance in immunoglobulin analysis or purification.

Examples of the alkoxythiocarbonylthio group having 1 to 6 carbon atoms represented by R ³ and R ^3a include methoxythiocarbonylthio group, ethoxythiocarbonylthio group, propoxythiocarbonylthio group, isopropoxythiocarbonylthio group, butoxythio group. Examples thereof include a carbonylthio group and a benzyloxythiocarbonylthio group.

  The aromatic group represented by Ar is not particularly limited, but furan-2-yl group, furan-3-yl group, thiophen-2-yl group, thiophen-3-yl group, pyrrol-2-yl group, Pyrrol-3-yl group, pyrazol-3-yl group, pyrazol-4-yl group, isothiazol-3-yl group, isothiazol-4-yl group, isoxazol-3-yl group, isoxazol-4-yl group Imidazol-2-yl group, imidazol-4-yl group, imidazol-5-yl group, oxazol-2-yl group, oxazol-4-yl group, oxazol-5-yl group, thiazol-2-yl group, Thiazol-4-yl group, thiazol-5-yl group, 1,2,4-triazol-3-yl group, phenyl group, 2-pyridyl group, 3-pyridyl group, 4-pyridyl group, 2-pi Midyl group, 4-pyrimidyl group, 5-pyrimidyl group, 1,2,4-triazin-3-yl group, 1,2,4-triazin-5-yl group, 1,2,4-triazin-6-yl Group, benzodioxolan-3-yl group, benzofuran-2-yl group, benzofuran-3-yl group, benzofuran-4-yl group, benzofuran-5-yl group, benzofuran-6-yl group, benzofuran-7-yl Group, benzothiophen-2-yl group, benzothiophen-3-yl group, benzothiophen-4-yl group, benzothiophen-5-yl group, benzothiophen-6-yl group, benzothiophen-7-yl group, Indol-2-yl group, indol-3-yl group, indol-4-yl group, indol-5-yl group, indol-6-yl group, indol-7-yl group Benzothiazol-2-yl group, benzothiazol-4-yl group, benzothiazol-5-yl group, benzothiazol-6-yl group, benzothiazol-7-yl group, indazol-3-yl group, indazole-4 -Yl group, indazol-5-yl group, indazol-6-yl group, indazol-7-yl group, benzisoxazol-3-yl group, benzisoxazol-4-yl group, benzisoxazole- 5-yl group, benzisoxazol-6-yl group, benzisoxazol-7-yl group, naphthalen-1-yl group, naphthalen-2-yl group, quinolin-2-yl group, quinolin-3- Yl group, quinolin-4-yl group, quinolin-5-yl group, quinolin-6-yl group, quinolin-7-yl group, quinolin-8-yl group, quinoxalinyl Group, quinoxalin-5-yl group, quinoxalin-6-yl group, quinazolinyl group, quinazolin-4-yl group, quinazolin-5-yl group, quinazolin-6-yl group, quinazolin-7-yl group, quinazoline-8 -Yl group, benzoxazin-5-yl group, benzoxazin-6-yl group, benzoxazin-7-yl group, benzoxazin-8-yl group, benzothiazin-5-yl group, benzothiazin-6-yl group, Benzothiazin-7-yl group, benzothiazin-8-yl group, 1,3-benzodioxol-4-yl group, 1,3-benzodioxol-5-yl group, 1,3-benzodioxole Examples include a -6-yl group, a 1,3-benzodioxol-7-yl group, and an anthracen-1-yl group. In particular, Ar is preferably a phenyl group, a 2-pyridyl group, a 3-pyridyl group, a 4-pyridyl group, or a furan-2-yl group in that it exhibits good performance in immunoglobulin analysis or purification.

  The substituent of the optionally substituted aromatic group represented by Ar is not particularly limited, but may be a halogen atom, an optionally substituted alkyl group having 1 to 6 carbon atoms, or an optionally substituted carbon. From an alkoxy group having 1 to 12 carbon atoms, an optionally substituted cycloalkoxy group having 3 to 8 carbon atoms, an optionally substituted alkenyloxy group having 3 to 6 carbon atoms, and an optionally substituted carbon number 3 6 alkynyloxy groups, optionally substituted acyloxy groups having 1 to 6 carbon atoms, optionally substituted alkylthio groups having 1 to 6 carbon atoms, optionally substituted alkylsulfinyl groups having 1 to 6 carbon atoms Group, optionally substituted alkylsulfonyl group having 1 to 6 carbon atoms, optionally substituted alkoxycarbonyl group having 1 to 12 carbon atoms, carboxyl group, nitro group Optionally substituted amino group, hydroxy group or cyano group is preferable.

  Examples of the halogen atom as the substituent for Ar include a fluorine atom, a chlorine atom, a bromine atom and an iodine atom.

  The alkyl group having 1 to 6 carbon atoms as a substituent for Ar may be linear, branched or cyclic, and is a methyl group, an ethyl group, a cyclopropyl group, a propyl group, an isopropyl group, Cyclopropyl group, butyl group, isobutyl group, sec-butyl group, tert-butyl group, pentyl group, isoamyl group, neopentyl group, 2-pentyl group, 3-pentyl group, 2-methylbutyl group, tert-pentyl group, hexyl Examples thereof include a group, an isohexyl group, a 2-hexyl group and a 3-hexyl group. These alkyl groups may be halogen atoms, cycloalkyl groups having 3 to 8 carbon atoms, alkoxy groups having 1 to 6 carbon atoms, alkoxycarbonyl groups having 1 to 6 carbon atoms, carboxyl groups, acyl groups, One or more good aromatic groups may be substituted, and more specifically, 2-chloroethyl group, 3-chloropropyl group, difluoromethyl group, 3-fluoropropyl group, methoxymethyl group, 2-ethoxyethyl group , Cyclopropylmethyl group, cyclopentylmethyl group, cyclohexylmethyl group, 2-methylthioethyl group, 2-ethylthioethyl group, 2-allylthioethyl group, 2-propargylthioethyl group, 2-benzylthioethyl group, 2- (2-chlorobenzyl) thioethyl group, 2- (2,4-dichlorobenzyl) thioethyl group, 2-methyl Sulfinylethyl group, 2-methylsulfonylethyl group, methoxymethyl group, ethoxymethyl group, 2-methoxyethyl group, 2-chloroethoxymethyl group, methoxycarbonylmethyl group, ethoxycarbonylmethyl group, 1-methoxycarbonylethyl group, 1 -Ethoxycarbonylethyl group, 2-ethoxycarbonylethyl group, carboxymethyl group, acetonyl group, 1-acetylethyl group, 3-acetylpropyl group, phenacyl group, 4-chlorophenacyl group, 2,4-difluorophenacyl group 4-methylphenacyl group, 4-isopropylphenacyl group, 4-isobutylphenacyl group, 4-cyclohexylphenacyl group, 4-cyanophenacyl group, 4-nitrophenacyl group, thiophen-2-yl group, 2- (Thiophen-2-yl) ethyl group, -(Thiophen-3-yl) ethyl group, furfuryl group, (5-methylfuran-2-yl) methyl group, (5-ethylfuran-2-yl) methyl group, (5-chlorofuran-2-yl) Methyl group, 2- (5-fluorofuran-2-yl) ethyl group, tetrahydrofurfuryl group, 2-pyridylmethyl group, 3-pyridylmethyl group, 4-pyridylmethyl group, 2- (pyridin-2-yl) Ethyl group, 2- (pyridin-3-yl) ethyl group, 2- (pyridin-4-yl) ethyl group, 3- (pyridin-2-yl) propyl group, 3- (pyridin-3-yl) propyl group , 3- (pyridin-4-yl) propyl group.

  The optionally substituted alkoxy group having 1 to 12 carbon atoms as the substituent for Ar may be linear or branched, and is a methoxy group, ethoxy group, propyloxy group, isopropyloxy Group, butoxy group, isobutyloxy group, sec-butyloxy group, tert-butoxy group, pentyloxy group, isoamyloxy group, neopentyloxy group, 2-pentyloxy group, 3-pentyloxy group, tert-pentyloxy group, Examples thereof include a hexyloxy group, an isohexyloxy group, a 2-hexyloxy group, a 3-hexyloxy group, and a decenyloxy group. These alkoxy groups may be halogen atoms, cycloalkyl groups having 3 to 8 carbon atoms, alkoxy groups having 1 to 6 carbon atoms, alkoxycarbonyl groups having 1 to 6 carbon atoms, carboxyl groups, acyl groups, One or more may be substituted with a good aromatic group, and more specifically, 2-chloroethoxy group, 3-chloropropyloxy group, difluoromethoxy group, 4-trifluoromethoxy group, 3-fluoropropyloxy Group, cyclopropylmethoxy group, cyclopentylmethoxy group, cyclohexylmethoxy group, 2-methylthioethoxy group, 2-ethylthioethoxy group, 2-allylthioethoxy group, 2-propargylthioethoxy group, 2-benzylthioethoxy group, 2 -(2-chlorobenzyl) thioethoxy group, 2- (2,4-dichlorobenzyl) ) Thioethoxy group, 2-methylsulfinylethoxy group, 2-methylsulfonylethoxy group, methoxymethoxy group, ethoxymethoxy group, 2-methoxyethoxy group, 2-chloroethoxymethoxy group, methoxycarbonylmethoxy group, ethoxycarbonylmethoxy group, 1 -Methoxycarbonylethoxy group, 1-ethoxycarbonylethoxy group, 2-ethoxycarbonylethoxy group, carboxymethoxy group, 1-acetylethoxy group, 3-acetylpropoxy group, 2-pyridylmethoxy group, 3-pyridylmethoxy group, 4- Pyridylmethoxy group, 2- (pyridin-2-yl) ethoxy group, 2- (pyridin-3-yl) ethoxy group, 2- (pyridin-4-yl) ethoxy group, 3- (pyridin-2-yl) propoxy Group, 3- (pyridin-3-yl) Propoxy group, can be exemplified benzyl group.

  Examples of the optionally substituted cycloalkoxy group having 3 to 8 carbon atoms as the substituent for Ar include a cyclopropyloxy group, a cyclobutyloxy group, a cyclopentyloxy group, a 2-methylcyclobutyloxy group, a cyclohexyloxy group, Examples include 2-methylcyclopentyloxy group, 3-methylcyclopentyloxy group, 4-methylcyclopentyloxy group, and cyclooctyloxy group.

  Examples of the optionally substituted alkenyloxy group having 3 to 6 carbon atoms as the substituent for Ar include allyloxy group, 2-methyl-2-propenyloxy group, 2-butenyloxy group, and 1-buten-3-yloxy group. , 3-butenyloxy group, 4-pentenyloxy group, and 5-hexenyloxy group. These alkenyloxy groups may be substituted with a halogen atom or the like, and examples thereof include a 3,3-difluoroallyloxy group, a 3,3-dichloroallyloxy group, and a 3,3-dibromoallyloxy group.

  Examples of the optionally substituted alkynyloxy group having 3 to 6 carbon atoms as a substituent for Ar include propargyloxy group, 1-butyn-3-yloxy group, 2-butynyloxy group, 3-butynyloxy group, and 2-pentyl. Nyloxy group, 3-pentynyloxy group, 4-pentynyloxy group, 2-hexynyloxy group, 3-hexynyloxy group, 4-hexynyloxy group, 5-hexynyloxy group can be exemplified, and these alkynyloxy groups are It may be substituted with a halogen atom or the like, and examples thereof include a 4,4,4-trifluorobutyn-2-yl group.

  Examples of the optionally substituted acyloxy group having 1 to 6 carbon atoms as a substituent for Ar include an acetoxy group and a propionyloxy group. These acyloxy groups may be substituted with a halogen atom or the like. A trifluoroacetoxy group, a 2,2,2-trifluoropropionyloxy group, etc. can be illustrated well.

  Examples of the optionally substituted alkylthio group having 1 to 6 carbon atoms as the substituent for Ar include a methylthio group, an ethylthio group, a propylthio group, an isopropylthio group, a butylthio group, an isobutylthio group, a sec-butylthio group, and a pentylthio group. , Isoamylthio group, neopentylthio group, 2-pentylthio group, 3-pentylthio group, 2-methylbutylthio group, hexylthio group, isohexylthio group, 3-methylpentylthio group, 2-methylpentylthio group can do. Further, one or more of these alkylthio groups may be substituted with a halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, and more specifically, a 2-chloroethylthio group, a 3-chloropropylthio group, a difluoro. Examples thereof include a methylthio group, a 3-fluoropropylthio group, a cyclopropylmethylthio group, a cyclopentylmethylthio group, and a cyclohexylmethylthio group.

  Examples of the optionally substituted alkylsulfinyl group having 1 to 6 carbon atoms as the substituent for Ar include methylsulfinyl group, ethylsulfinyl group, propylsulfinyl group, isopropylsulfinyl group, butylsulfinyl group, isobutylsulfinyl group, sec- Butylsulfinyl group, pentylsulfinyl group, isoamylsulfinyl group, neopentylsulfinyl group, 2-pentylsulfinyl group, 3-pentylsulfinyl group, 2-methylbutylsulfinyl group, hexylsulfinyl group, isohexylsulfinyl group, 3-methylpentylsulfinyl group Examples thereof include a 2-methylpentylsulfinyl group. One or more of these alkylsulfinyl groups may be substituted with a halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, and more specifically, 2-chloroethylsulfinyl group, 3-chloropropylsulfinyl group, Examples thereof include a difluoromethylsulfinyl group, a 3-fluoropropylsulfinyl group, a cyclopropylmethylsulfinyl group, a cyclopentylmethylsulfinyl group, and a cyclohexylmethylsulfinyl group.

  Examples of the optionally substituted alkylsulfonyl group having 1 to 6 carbon atoms as the substituent for Ar include a methylsulfonyl group, an ethylsulfonyl group, a propylsulfonyl group, an isopropylsulfonyl group, a butylsulfonyl group, an isobutylsulfonyl group, sec- Butylsulfonyl group, pentylsulfonyl group, isoamylsulfonyl group, neopentylsulfonyl group, 2-pentylsulfonyl group, 3-pentylsulfonyl group, 2-methylbutylsulfonyl group, hexylsulfonyl group, isohexylsulfonyl group, 3-methylpentylsulfonyl Examples thereof include a 2-methylpentylsulfonyl group. In addition, one or more of these alkylsulfonyl groups may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms, and more specifically, 2-chloroethylsulfonyl group, 3-chloropropylsulfonyl group, difluoro Examples thereof include a methylsulfonyl group, a 3-fluoropropylsulfonyl group, a cyclopropylmethylsulfonyl group, a cyclopentylmethylsulfonyl group, and a cyclohexylmethylsulfonyl group.

  Examples of the optionally substituted alkoxycarbonyl group having 1 to 12 carbon atoms as the substituent for Ar include a methoxycarbonyl group, an ethoxycarbonyl group, a propoxycarbonyl group, a butoxycarbonyl group, a hexyloxycarbonyl group, a dodecyloxycarbonyl group, A benzyloxycarbonyl group and a tert-butoxycarbonyl group can be exemplified, and one or more of these alkoxycarbonyl groups may be substituted with a halogen atom or the like, more specifically, a trifluoromethoxycarbonyl group, 2, 2 , 2-trifluoroethoxycarbonyl group and the like.

  Examples of the optionally substituted amino group as the substituent for Ar include an amino group, a methylamino group, a dimethylamino group, an ethylamino group, an N-ethyl-N-methylamino group, a diethylamino group, and an N-methyl-N. -Propylamino group, N-ethyl-N-propylamino group, 2,2,2-trifluoroethylamino group, dipropylamino group, isopropylamino group, N-methyl-N-isopropylamino group, butylamino group, N-butyl-N-methylamino group, isobutylamino group, sec-butylamino group, pentylamino group, isoamylamino group, neopentylamino group, 2-pentylamino group, 3-pentylamino group, 2-methylbutylamino Group, hexylamino group, isohexylamino group, 4-methylpentylamino group, benzenesulfonyl group Group, N-benzenesulfonyl-N-methylamino group, p-toluenesulfonylamino group, 4-chlorobenzenesulfonylamino group, 4-nitrobenzenesulfonylamino group, methylsulfonylamino group, chloromethylsulfonylamino group, trifluoromethylsulfonyl Amino group, N-methylsulfonyl-N-methylamino group, N-methylsulfonyl-N-ethylamino group, N-methylsulfonyl-N-propylamino group, ethylsulfonylamino group, N-ethylsulfonyl-N-methylamino Group, N-ethylsulfonyl-N-ethylamino group, methoxycarbonylamino group, ethoxycarbonylamino group can be exemplified.

Specific examples of the optionally substituted aromatic group represented by Ar include furan-2-yl group, 5-bromofuran-2-yl group, 5-methylfuran-2-yl group, and furan-3-yl. Group, thiophen-2-yl group, 5-chlorothiophen-2-yl group, 5-bromothiophen-2-yl group, 5-methylthiophen-2-yl group, 5-methoxythiophen-2-yl group, 5 -Ethoxythiophen-2-yl group, thiophen-3-yl group, 5-methylthiophen-3-yl group, 5-methoxythiophen-3-yl group, 5-ethoxythiophen-3-yl group, 1-methylpyrrole 2-yl group, 1-methylpyrrol-3-yl group, 1-ethylpyrazol-3-yl group, pyrazol-2-yl group, 3-ethoxy-1-methylpyrazol-2-yl group, 1-ethyl 4-methoxypyrazol-3-yl group, isothiazol-3-yl group, 5-methoxyisothiazol-3-yl group, isothiazol-4-yl group, isoxazol-3-yl group, isoxazol-4-yl group 1-methylimidazol-2-yl group, 1-methylimidazol-4-yl group, 1-methylimidazol-5-yl group, oxazol-2-yl group, oxazol-4-yl group, oxazol-5-yl Group, thiazol-2-yl group, 3-methoxythiazol-2-yl group, 4-methoxythiazol-2-yl group, thiazol-4-yl group, thiazol-5-yl group, 1,2,4-triazole -3-yl group, 1,2,4-triazol-5-yl group, phenyl group, 2-fluorophenyl group, 3-fluorophenyl group, 4-fluoro Phenyl group, 2,3-difluorophenyl group, 3,5-difluorophenyl group, 3,4,5-trifluorophenyl group, 2,3,4,5,6-pentafluorophenyl group, 3-fluoro-4 -Methylphenyl group, 4-trifluoromethoxyphenyl group, 2-chlorophenyl group, 3-chlorophenyl group, 4-chlorophenyl group, 4-chloro-3-methylphenyl group, 4-chloro-3-ethylphenyl group, 4- Chloro-3-methoxyphenyl group, 4-chloro-3-ethoxyphenyl group, 2,3-dichlorophenyl group, 3,5-dichlorophenyl group, 3,4,5-trichlorophenyl group, 3-trifluoromethylphenyl group, 4-trifluoromethylphenyl group, 3-bromophenyl group, 4-bromophenyl group, 4-bromo-3-methylphenyl group 4-bromo-3-ethylphenyl group, 4-bromo-3-methoxyphenyl group, 4-bromo-3-ethoxyphenyl group, 3,5-dibromo-4-hydroxyphenyl group, 3-iodophenyl group, 4 -Iodophenyl group, 4-iodo-3-methylphenyl group, 4-iodo-3-ethylphenyl group, 4-iodo-3-methoxyphenyl group, 4-iodo-3-ethoxyphenyl group, 2-methylphenyl group 3-methylphenyl group, 4-methylphenyl group, 3,4-dimethylphenyl group, 3,4,5-trimethylphenyl group, 3-hydroxy-4-methylphenyl group, 2-trifluorophenylmethylphenyl group, 3-trifluorophenylmethylphenyl group, 4-trifluorophenylmethylphenyl group, 4-methoxymethylphenyl group, 3,4- Ethoxyphenyl group, 3-ethyl-4-methylphenyl group, 4-ethyl-3-methylphenyl group, 4-ethylphenyl group, 3,4-diethylphenyl group, 4-propylphenyl group, 4-isopropylphenyl group, 3-butylphenyl group, 4-butylphenyl group, 4-isobutylphenyl group, 4- (sec-butyl) phenyl group, 4- (tert-butyl) phenyl group, 4-pentylphenyl group, 4-isoamylphenyl group, 4-neopentylphenyl group, 4- (2-pentyl) phenyl group, 4- (3-pentyl) phenyl group, 4- (2-methylbutyl) phenyl group, 4- (tert-pentyl) phenyl group, 4-hexyl Phenyl group, 4-isohexylphenyl group, 4- (2-chloroethyl) phenyl group, 4- (3-chloropropyl) phenol Group, 4- (difluoromethyl) phenyl group, 4- (3-fluoropropyl) phenyl group, 4-methoxymethylphenyl group, 4-ethoxymethylphenyl group, 4-cyclopropylmethylphenyl group, 4-cyclopentylmethylphenyl Group, cyclohexylmethylphenyl group, 4- (2-methylthioethyl) phenyl group, 4- (2-ethylthioethyl) phenyl group, 4- (2-allylthioethyl) phenyl group, 4- (2-propargylthioethyl) ) Phenyl group, 4- (2-benzylthioethyl) phenyl group, 3-methoxyphenyl group, 3-methoxy-4-methylphenyl group, 4-ethyl-3-methoxyphenyl group, 3-methoxy-4-propylphenyl Group, 4-methoxyphenyl group, 4-methoxy-3-methoxyphenyl group, 3,4-dimethoxy Siphenyl group, 3-ethoxy-4-methoxyphenyl group, 4-ethoxy-3-methoxyphenyl group, 4-isopropyloxy-3-methoxyphenyl group, 3-isopropyloxy-4-methoxyphenyl group, 3-ethoxyphenyl group 4-ethoxyphenyl group, 4-ethoxy-3-methylphenyl group, 4-ethoxy-3-ethylphenyl group, 4-ethoxy-3-propylphenyl group, 4-ethoxy-3-isopropylphenyl group, 4-propyl Oxyphenyl group, 4-isopropyloxyphenyl group, 4-butoxyphenyl group, 4-isobutyloxyphenyl group, 4- (sec-butyloxy) phenyl group, 3- (2-chloroethoxy) phenyl group, 3- (2- Chloroethoxy) phenyl group, 4- (2-chloroethoxy) phenyl group, 3- ( -Chloropropyloxy) phenyl group, 4- (3-chloropropyloxy) phenyl group, 3-difluoromethoxyphenyl group, 4-difluoromethoxyphenyl group, 4- (3-fluoropropyloxyphenyl group), 4-cyclopropyl Methoxyphenyl group, 4-cyclopentylmethoxy group, 4-cyclohexylmethoxy group, 3-cyclopropyloxyphenyl group, 4-cyclopropyloxyphenyl group, 4-cyclopropyloxy-3-methoxyphenyl group, 4-cyclopropyloxy- 3-methylphenyl group, 4-cyclobutyloxyphenyl group, 4-cyclopentyloxyphenyl group, 4- (2-methylcyclobutyloxy) phenyl group, 4-cyclohexyloxyphenyl, 4-allyloxyphenyl group, 4- ( 3,3-dichloro Ryloxy) phenyl group, 4-propargyloxyphenyl group, 3-methyl-4-propargyloxyphenyl group, 3-ethyl-4-propargyloxyphenyl group, 3-methoxy-4-propargyloxyphenyl group, 3-ethoxy-4 -Propargyloxyphenyl group, 3-propoxy-4-propargyloxyphenyl group, 4- (1-butyn-3-yloxy) phenyl group, 4- (2-butynyloxy) phenyl group, 3-methylthiophenyl group, 4-methylthio Phenyl group, 4-methyl-3-methylthiophenyl group, 3-methyl-4-methylthiophenyl group, 4-methoxy-3-methylthiophenyl group, 3-ethoxy-4-methylthiophenyl group, 4-ethoxy-3-methylthio Phenyl group, 3-propoxy-4-methylthiophenyl Group, 4-ethylthiophenyl group, 3-propylthiophenyl group, 4-isopropylthiophenyl group, 4-butylthiophenyl group, 4-isobutylthiophenyl group, 2- (sec-butylthio) phenyl group, 2-penthi Ruthiophenyl group, 2-isoamylthiophenyl group, 4-neopentylthiophenyl group, 4- (2-chloroethylthio) phenyl group, 3- (3-chloropropylthio) phenyl group, difluoromethylthiophenyl group, 3- Acetoxyphenyl group, 4-acetoxyphenyl group, 3-methylsulfinylphenyl group, 4-methylsulfinylphenyl group, 4-methoxy-3-methylsulfinylphenyl group, 3-methoxy-4-methylsulfinylphenyl group, 4-ethoxy- 3-methylsulfinylphenyl group, 3-ethoxy- -Methylsulfinylphenyl group, 4-methyl-3-methylsulfinylphenyl group, 3-ethyl-4-methylsulfinylphenyl group, 4- (2-chloroethylsulfinyl) phenyl group, 4- (3-chloropropylsulfinyl) phenyl Group, 4- (difluoromethylsulfinyl) phenyl group, 3-methylsulfonylphenyl group, 4-methylsulfonylphenyl group, 4-methyl-3-methylsulfonylphenyl group, 4-ethyl-3-methylsulfonylphenyl group, 4- Methoxy-3-methylsulfonylphenyl group, 4-ethoxy-3-methylsulfonylphenyl group, 3-methoxy-4-methylsulfonylphenyl group, 3-ethoxy-4-methylsulfonylphenyl group, ethylsulfonylphenyl group, propylsulfonylphenyl Group, Sopropylsulfonylphenyl group, butylsulfonylphenyl group, 2-chloroethylsulfonylphenyl group, 3-chloropropylsulfonylphenyl group, difluoromethylsulfonylphenyl group, 4-carboxyphenyl group, 4-methoxycarbonylphenyl group, 4-ethoxycarbonyl Phenyl group, 3-methylaminophenyl group, 4-methylaminophenyl group, 4-methoxy-3-methylaminophenyl group, 3-methoxy-4-methylaminophenyl group, 4-ethoxy-3-methylaminophenyl group, 3-ethoxy-4-methylaminophenyl group, 4-methyl-3-methylaminophenyl group, 3-methyl-4-methylaminophenyl group, 4-ethyl-3-methylaminophenyl group, 3-ethyl-4- Methylaminophenyl group, 3-dimethyl Aminophenyl group, 4-dimethylaminophenyl group, 4-methoxy-3-dimethylaminophenyl group, 3-methoxy-4-dimethylaminophenyl group, 4-ethoxy-3-dimethylaminophenyl group, 3-ethoxy-4- Dimethylaminophenyl group, 4-methyl-3-dimethylaminophenyl group, 3-methyl-4-dimethylaminophenyl group, 4-ethyl-3-dimethylaminophenyl group, 3-methyl-4-dimethylaminophenyl group, 3 -(N-ethyl-N-methylamino) phenyl group, 4- (N-ethyl-N-methylamino) phenyl group, 4-methoxy-3- (N-ethyl-N-methylamino) phenyl group, 3- Methoxy-4- (N-ethyl-N-methylamino) phenyl group, 4-ethoxy-3- (N-ethyl-N-methylamino) phenyl Group, 3-ethoxy-4- (N-ethyl-N-methylamino) phenyl group, 4-methyl-3- (N-ethyl-N-methylamino) phenyl group, 3-methyl-4- (N- Ethyl-N-methylamino) phenyl group, 4-ethyl-3- (N-ethyl-N-methylamino) phenyl group, 3-methyl-4- (N-ethyl-N-methylamino) phenyl group, 3- Diethylaminophenyl group, 4-diethylaminophenyl group, 4-methoxy-3-diethylaminophenyl group, 3-methoxy-4-diethylaminophenyl group, 4-ethoxy-3-diethylaminophenyl group, 3-ethoxy-4-diethylaminophenyl group, 4-methyl-3-diethylaminophenyl group, 3-methyl-4-diethylaminophenyl group, 4-ethyl-3-diethylaminopheny Group, 3-methyl-4-diethylaminophenyl group, 4-methylsulfonylaminophenyl group, 4-trifluoromethylsulfonylaminophenyl group, 4-acetylaminophenyl group, 2-hydroxyphenyl group, 3-hydroxyphenyl group, 4 -Hydroxyphenyl group, 3-hydroxy-4-methylphenyl group, 3-hydroxy-4-ethylphenyl group, 3-hydroxy-4-methoxyphenyl group, 4-hydroxy-3-methylphenyl group, 4-hydroxy-3 -Ethylphenyl group, 4-hydroxy-3-methoxyphenyl group, 2-cyanophenyl group, 3-cyanophenyl group, 4-cyanophenyl group, 2-nitrophenyl group, 3-nitrophenyl group, 3-nitro-4 -Methylphenyl group, 4-nitrophenyl group, pyridin-2-yl group, 5- Chloropyridin-2-yl group, 5-methylpyridin-2-yl group, 5-ethylpyridin-2-yl group, 5-methoxypyridin-2-yl group, 5-ethoxypyridin-2-yl group, 2, 3-dimethylpyridin-6-yl group, 2,3-dimethoxypyridin-6-yl group, pyridyl-3-yl group, 2-chloropyridin-5-yl group, 2-methylpyridin-5-yl group, 2 -Ethylpyridin-5-yl group, 2-methoxypyridin-5-yl group, 2-ethoxypyridin-5-yl group, 2,3-dimethylpyridin-5-yl group, pyridyl-4-yl group, 2- Methylpyridin-4-yl group, 2-ethylpyridin-4-yl group, 2-methoxypyridin-4-yl group, 2-ethoxypyridin-4-yl group, 2-pyrimidyl group, 5-methylpyrimidin-2- Il group 5-ethylpyrimidin-2-yl group, 2-ethoxypyridin-5-yl group, 2,3-dimethylpyridin-5-yl group, 2-methylpyridin-4-yl group, 2-ethylpyridin-4-yl Group, 2-methoxypyridin-4-yl group, 2-ethoxypyridin-4-yl group, 5-chloropyrimidin-2-yl group, 5-bromopyrimidin-2-yl group, 5-methoxypyrimidin-2-yl Group, pyrimidyl-4-yl group, pyrimidyl-5-yl group, 1,2,4-triazin-3-yl group, 1,2,4-triazin-5-yl group, 1,2,4-triazine- 6-yl group, benzodioxolan-3-yl group, benzofuran-2-yl group, benzofuran-3-yl group, benzofuran-4-yl group, benzofuran-5-yl group, benzofuran-6-yl group, ben Furan-7-yl group, benzothiophen-2-yl group, benzothiophen-3-yl group, benzothiophen-4-yl group, benzothiophen-5-yl group, benzothiophen-6-yl group, benzothiophene- 7-yl group, 1-methylindol-2-yl group, benzothiazol-2-yl group, benzothiazol-4-yl group, benzothiazol-5-yl group, benzothiazol-6-yl group, benzothiazol- 7-yl group, benzisoxazol-3-yl group, benzisoxazol-4-yl group, benzisoxazol-5-yl group, benzisoxazol-6-yl group, benzisoxazole-7 -Yl group, naphthalen-1-yl group, naphthalen-2-yl group, quinolin-2-yl group, quinolin-3-yl group, quinolin-4-yl group, Quinolin-5-yl group, quinolin-6-yl group, quinolin-7-yl group, quinolin-8-yl group, quinoxalin-5-yl group, quinoxalin-6-yl group, quinazolin-4-yl group, quinazoline -5-yl group, quinazolin-6-yl group, quinazolin-7-yl group, quinazolin-8-yl group, benzoxazin-5-yl group, benzoxazin-6-yl group, benzoxazin-7-yl group Benzoxazin-8-yl group, benzothiazin-5-yl group, benzothiazin-6-yl group, benzothiazin-7-yl group, benzothiazin-8-yl group, 1,3-benzodioxol-4-yl group 1,3-benzodioxol-5-yl group, anthracen-1-yl group and the like.

  Ar is a 4-methylphenyl group, a 4-ethylphenyl group, a 3,4-dimethylphenyl group, a 3-ethyl-4-methylphenyl group, a 4-ethyl group because it exhibits good performance in the analysis or purification of immunoglobulins. -3-methylphenyl group, 4-methoxyphenyl group, 4-ethoxyphenyl group, 3,4-dimethoxyphenyl group, 3,4-diethoxyphenyl group, 3-ethoxy-4-methoxyphenyl group, 4-ethoxy- 3-methoxyphenyl group, 4-methoxy-3-methylphenyl group, 4-methyl-3-ethoxyphenyl group, 4-dimethylaminophenyl group, 2-methoxypyridin-5-yl group, 2-ethoxypyridine-5- Yl group, 2,3-dimethylpyridin-5-yl group, 4-pyridyl group, 2-methylpyridin-4-yl group, 2-ethylpyridine-4 Yl group, 2-methoxy-pyridin-4-yl group, 2-ethoxy-4-yl group, 2-chloropyridin-5-yl group.

Examples of the alkyl group having 1 to 6 carbon atoms represented by R ⁴ include a methyl group, an ethyl group, a propyl group, and a butyl group, and these alkyl groups are substituted by one or more halogen atoms. More specifically, a 2,2,2-trifluoroethyl group, a benzyl group, and the like can be exemplified.

  Examples of the halogen group represented by Y include a chlorine atom, a bromine atom, a methylsulfonyloxy group, and a tosyl group. A bromine atom is preferable in that the yield of the target product is good.

  n can be appropriately selected from an integer of 1 to 12, but 3, 4, 5 or 6 is preferable in that the antibody capture ability is high and the selectivity with a contaminating protein is good.

  Next, the manufacturing method of the thiazole derivative of this invention is demonstrated in detail.

  Manufacturing method 1

（式中、Ｒ^１、Ｒ^２、Ｒ^３ａ、Ａｒ、Ｙ及びｎは前記と同じ意味を表す。）
製造方法１はα−ハロケトン体（２）をアシルチオウレア誘導体（３）と反応させ、チアゾール誘導体（１ａ）を製造する方法である。本反応は溶媒中で行なうことが好ましく、反応に害を及ぼさない溶媒であれば使用することができる。溶媒としてはジエチルエーテル、テトラヒドロフラン（ＴＨＦ）、１，２−ジメトキシエタン（ＤＭＥ）、ジオキサン等のエーテル系溶媒、アセトン、メチルエチルケトン等のケトン系溶媒、酢酸エチル、酢酸ブチル等のエステル系溶媒、アセトニトリル、プロピオニトリル等のニトリル類、ベンゼン、トルエン、キシレン、クロロベンゼン等の芳香族炭化水素系溶媒、Ｎ，Ｎ−ジメチルホルムアミド（ＤＭＦ）、Ｎ−メチルピロリドン等のアミド類、ジメチルスルホキシド（ＤＭＳＯ）、水又はこれらの混合溶媒が例示でき、中でも収率がよい点で、ＤＭＦを用いるのが好ましい。反応温度については特に制限はないが、０℃から１５０℃の範囲から適宜選ばれた温度で反応させることにより目的物を得ることができ、収率がよい点で６０℃から１５０℃の範囲で反応させるのが好ましい。本工程では、塩基存在下に行なうこともでき、塩基としては、水素化ナトリウム、ナトリウムアミド、炭酸ナトリウム、炭酸カリウム、ナトリウムメトキシド、ナトリウムエトキシド、カリウム−ｔｅｒｔ−ブトキシド、水酸化ナトリウム、水酸化カリウム等のアルカリ金属塩基、トリエチルアミン、トリブチルアミン、Ｎ−メチルモルホリン、ピリジン、ジメチルアニリン等の有機アミン類を用いることができる。塩基の使用量は特に制限はないが、反応基質に対して１当量以上用いて反応を実施することにより、収率よく目的物を得ることができる。反応終了後は、通常の後処理操作により目的物を得ることができるが、必要であればカラムクロマトグラフィー又は再結晶等の方法により精製することもできる。

(Wherein R ¹ , R ² , R ^3a , Ar, Y and n have the same meaning as described above).
Production method 1 is a method in which an α-haloketone body (2) is reacted with an acylthiourea derivative (3) to produce a thiazole derivative (1a). This reaction is preferably carried out in a solvent, and any solvent that does not harm the reaction can be used. Solvents include ether solvents such as diethyl ether, tetrahydrofuran (THF), 1,2-dimethoxyethane (DME), dioxane, ketone solvents such as acetone and methyl ethyl ketone, ester solvents such as ethyl acetate and butyl acetate, acetonitrile, Nitriles such as propionitrile, aromatic hydrocarbon solvents such as benzene, toluene, xylene, chlorobenzene, amides such as N, N-dimethylformamide (DMF) and N-methylpyrrolidone, dimethyl sulfoxide (DMSO), water Alternatively, a mixed solvent thereof can be exemplified, and it is preferable to use DMF because it has a good yield. Although there is no restriction | limiting in particular about reaction temperature, The target object can be obtained by making it react at the temperature chosen suitably from the range of 0 degreeC to 150 degreeC, and it is the range of 60 degreeC to 150 degreeC by the point with a sufficient yield. It is preferable to react. This step can also be carried out in the presence of a base. Examples of the base include sodium hydride, sodium amide, sodium carbonate, potassium carbonate, sodium methoxide, sodium ethoxide, potassium tert-butoxide, sodium hydroxide, hydroxide Alkali metal bases such as potassium, organic amines such as triethylamine, tributylamine, N-methylmorpholine, pyridine and dimethylaniline can be used. The amount of the base used is not particularly limited, but the target product can be obtained in good yield by carrying out the reaction using 1 equivalent or more of the reaction substrate. After completion of the reaction, the desired product can be obtained by ordinary post-treatment operations, but if necessary, it can be purified by a method such as column chromatography or recrystallization.

  Manufacturing method 2

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）
製造方法２はチアゾール誘導体（１ａａ）を水存在下にてヘキサカルボニルモリブテンで処理し、５−ヒドロキシアルキルチアゾール誘導体（１ａｂ）を製造する方法である。水及びヘキサカルボニルモリブテンの使用量は反応基質（１ａａ）に対して１当量以上用いて反応を実施することにより、収率よく目的物（１ａｂ）を得ることができる。本反応は溶媒中で行なうのが好ましく、反応に害を及ぼさない溶媒であれば使用することができる。溶媒としては、ジエチルエーテル、ＴＨＦ、ＤＭＥ、ジオキサン等のエーテル系溶媒、アセトン、メチルエチルケトン等のケトン系溶媒、酢酸エチル、酢酸ブチル等のエステル系溶媒、アセトニトリル、プロピオニトリル等のニトリル類、ベンゼン、トルエン、キシレン、クロロベンゼン等の芳香族炭化水素系溶媒、ＤＭＦ、Ｎ−メチルピロリドン等のアミド系溶媒、メタノール、エタノール、イソプロピルアルコール等のアルコール系溶媒、ＤＭＳＯ、又はこれらの混合溶媒が例示でき、中でも収率がよい点や後処理が簡便である点でアセトニトリル、プロピオニトリル等のニトリル類溶媒を用いることが好ましい。反応温度については特に制限はないが、０℃から１５０℃の範囲から適宜選ばれた温度で反応させることにより目的物を得ることができる。反応終了後は、通常の後処理操作により目的物を得ることができるが、必要であればカラムクロマトグラフィー又は再結晶等の方法により精製することもできる。

(In the formula, R ¹ , R ² , Ar and n represent the same meaning as described above.)
Production method 2 is a method for producing a 5-hydroxyalkylthiazole derivative (1ab) by treating a thiazole derivative (1aa) with hexacarbonylmolybten in the presence of water. The target product (1ab) can be obtained in good yield by carrying out the reaction using water and hexacarbonylmolybten in an amount of 1 equivalent or more with respect to the reaction substrate (1aa). This reaction is preferably carried out in a solvent, and any solvent that does not harm the reaction can be used. Solvents include ether solvents such as diethyl ether, THF, DME, dioxane, ketone solvents such as acetone and methyl ethyl ketone, ester solvents such as ethyl acetate and butyl acetate, nitriles such as acetonitrile and propionitrile, benzene, Examples include aromatic hydrocarbon solvents such as toluene, xylene and chlorobenzene, amide solvents such as DMF and N-methylpyrrolidone, alcohol solvents such as methanol, ethanol and isopropyl alcohol, DMSO, and mixed solvents thereof. Nitriles solvents such as acetonitrile and propionitrile are preferably used in terms of good yield and simple post-treatment. Although there is no restriction | limiting in particular about reaction temperature, A target object can be obtained by making it react at the temperature chosen suitably from the range of 0 to 150 degreeC. After completion of the reaction, the desired product can be obtained by ordinary post-treatment operations, but if necessary, it can be purified by a method such as column chromatography or recrystallization.

  Manufacturing method 3

（式中、Ｒ^１、Ｒ^２、Ｒ^４、Ａｒ及びｎは前記と同じ意味を表す。）
製造方法３はチアゾール誘導体（１ａｃ）を塩基で処理し、５−メルカプトアルキルチアゾール誘導体（１ａｄ）を製造する方法である。本反応で用いることができる塩基としては、水素化ナトリウム、ナトリウムアミド、炭酸ナトリウム、炭酸カリウム、ナトリウムメトキシド、ナトリウムエトキシド、カリウム−ｔｅｒｔ−ブトキシド、水酸化ナトリウム、水酸化カリウム等のアルカリ金属塩基、メチルアミン、エチルアミン、プロピルアミン、ジメチルアミン、ジエチルアミン等の有機アミン類、又はアンモニアを用いることができる。塩基の使用量は特に制限はないが、反応基質（１ａｃ）に対して１当量以上用いて反応を実施することにより、収率よく目的物（１ａｄ）を得ることができる。本反応は溶媒中で行なうことが好ましく、反応に害を及ぼさない溶媒であれば使用することができる。溶媒としてはジエチルエーテル、ＴＨＦ、ＤＭＥ、ジオキサン等のエーテル系溶媒、アセトン、メチルエチルケトン等のケトン系溶媒、酢酸エチル、酢酸ブチル等のエステル系溶媒、アセトニトリル、プロピオニトリル等のニトリル類、ベンゼン、トルエン、キシレン、クロロベンゼン等の芳香族炭化水素系溶媒、ＤＭＦ、Ｎ−メチルピロリドン等のアミド類、ＤＭＳＯ、水又はこれらの混合溶媒が例示でき、中でも収率がよい点で、ＤＭＦを用いるのが好ましい。また、塩基としてアンモニアを用い、使用する溶媒として水とエタノールの混合溶媒を用いて実施しても収率がよい点で好ましい。また、反応温度については特に制限はないが、０℃から１５０℃の範囲から適宜選ばれた温度で反応させることにより目的物を得ることができ、収率がよい点で０℃から１００℃の範囲で反応させることが好ましい。反応終了後は、通常の後処理操作により目的物を得ることができるが、必要であればカラムクロマトグラフィー又は再結晶等の方法により精製することもできる。

(In the formula, R ¹ , R ² , R ⁴ , Ar and n have the same meaning as described above.)
Production method 3 is a method for producing a 5-mercaptoalkylthiazole derivative (1ad) by treating a thiazole derivative (1ac) with a base. Bases that can be used in this reaction include alkali metal bases such as sodium hydride, sodium amide, sodium carbonate, potassium carbonate, sodium methoxide, sodium ethoxide, potassium tert-butoxide, sodium hydroxide, potassium hydroxide and the like. , Organic amines such as methylamine, ethylamine, propylamine, dimethylamine, and diethylamine, or ammonia can be used. The amount of base used is not particularly limited, but the target product (1ad) can be obtained in good yield by carrying out the reaction using 1 equivalent or more of the reaction substrate (1ac). This reaction is preferably carried out in a solvent, and any solvent that does not harm the reaction can be used. Solvents include ether solvents such as diethyl ether, THF, DME and dioxane, ketone solvents such as acetone and methyl ethyl ketone, ester solvents such as ethyl acetate and butyl acetate, nitriles such as acetonitrile and propionitrile, benzene and toluene. In addition, aromatic hydrocarbon solvents such as xylene and chlorobenzene, amides such as DMF and N-methylpyrrolidone, DMSO, water, or a mixed solvent thereof can be exemplified. Among them, DMF is preferably used because of its good yield. . Moreover, it is preferable at a point with a good yield, even if it implements using ammonia as a base and using the mixed solvent of water and ethanol as a solvent to be used. The reaction temperature is not particularly limited, but the target product can be obtained by reacting at a temperature appropriately selected from the range of 0 ° C to 150 ° C, and the yield is good from 0 ° C to 100 ° C. It is preferable to make it react in the range. After completion of the reaction, the desired product can be obtained by ordinary post-treatment operations, but if necessary, it can be purified by a method such as column chromatography or recrystallization.

  Manufacturing method 4

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）
製造方法４は５−ヒドロキシアルキルチアゾール誘導体（１ａｂ）のヒドロキシ基を酸化し、５−ホルミルアルキルチアゾール誘導体（１ａｅ）を製造する方法である。本反応は一般的な酸化反応を行なうことによって容易に目的物を得ることができ、酸化剤としては、ピリジニウムクロロクロマート、ピリジニウムジクロロクロマート、二酸化マンガン、過マンガン酸カリウム等があげられる。また、Ｓｗｅｒｎ酸化、Ｃｏｒｅｙ−Ｋｉｍ酸化、Ｄｅｓｓ−Ｍａｒｔｉｎ酸化、向山酸化、Ｏｐｐｅｎａｕｅｒ酸化、ＴＥＭＰＯ酸化、パラジウム錯体を用いた酸素酸化等の酸化反応によっても容易に目的物を得ることができるが、収率がよい点でＳｗｅｒｎ酸化を用いることが好ましい。本反応は溶媒中で行なうことが好ましく、反応に害を及ぼさない溶媒であれば使用することができる。溶媒としてはジクロロメタン、クロロホルム、１，２−ジクロロエタン等のハロゲン系溶媒、ジエチルエーテル、ＴＨＦ、ＤＭＥ、ジオキサン等のエーテル系溶媒、アセトン、メチルエチルケトン等のケトン系溶媒、酢酸エチル、酢酸ブチル等のエステル系溶媒、アセトニトリル、プロピオニトリル等のニトリル類、ベンゼン、トルエン、キシレン、クロロベンゼン等の芳香族炭化水素系溶媒、ＤＭＦ、Ｎ−メチルピロリドン等のアミド類、ＤＭＳＯ、水又はこれらの混合溶媒が例示でき、中でも収率がよい点で、ジクロロメタン、クロロホルム、１，２−ジクロロエタン等のハロゲン系溶媒を用いるのが好ましい。反応温度については特に制限はないが、−７８℃から１５０℃の範囲から適宜選ばれた温度で反応させることにより目的物を得ることができる。反応終了後は、通常の後処理操作により目的物を得ることができるが、必要であればカラムクロマトグラフィー又は再結晶等により精製することもできる。

(In the formula, R ¹ , R ² , Ar and n represent the same meaning as described above.)
Production method 4 is a method for producing a 5-formylalkylthiazole derivative (1ae) by oxidizing the hydroxy group of the 5-hydroxyalkylthiazole derivative (1ab). In this reaction, the desired product can be easily obtained by carrying out a general oxidation reaction. Examples of the oxidizing agent include pyridinium chlorochromate, pyridinium dichlorochromate, manganese dioxide, potassium permanganate and the like. Further, the target product can be easily obtained by an oxidation reaction such as Swern oxidation, Corey-Kim oxidation, Dess-Martin oxidation, Mukaiyama oxidation, Openauer oxidation, TEMPO oxidation, oxygen oxidation using a palladium complex. However, it is preferable to use Swern oxidation because of its good point. This reaction is preferably carried out in a solvent, and any solvent that does not harm the reaction can be used. Solvents include halogen solvents such as dichloromethane, chloroform, 1,2-dichloroethane, ether solvents such as diethyl ether, THF, DME, and dioxane, ketone solvents such as acetone and methyl ethyl ketone, and ester solvents such as ethyl acetate and butyl acetate. Examples include solvents, nitriles such as acetonitrile and propionitrile, aromatic hydrocarbon solvents such as benzene, toluene, xylene and chlorobenzene, amides such as DMF and N-methylpyrrolidone, DMSO, water or a mixed solvent thereof. Among them, it is preferable to use a halogen-based solvent such as dichloromethane, chloroform, 1,2-dichloroethane or the like in terms of a good yield. Although there is no restriction | limiting in particular about reaction temperature, A target object can be obtained by making it react at the temperature suitably selected from the range of -78 degreeC to 150 degreeC. After completion of the reaction, the desired product can be obtained by ordinary post-treatment operation, but can be purified by column chromatography or recrystallization if necessary.

  Manufacturing method 5

（式中、Ｒ^１、Ｒ^２、Ａｒ及びｎは前記と同じ意味を表す。）
製造方法５はチアゾール誘導体（１ａａ）を塩基で処理し、５−アミノキシアルキルチアゾール誘導体（１ａａａ）を製造する方法である。塩基としては、水素化ナトリウム、ナトリウムアミド、炭酸ナトリウム、炭酸カリウム、ナトリウムメトキシド、ナトリウムエトキシド、カリウム−ｔｅｒｔ−ブトキシド、水酸化ナトリウム、水酸化カリウム等のアルカリ金属塩基、メチルアミン、エチルアミン、ジメチルアミン等の有機アミン類、ヒドラジン類を用いることができ、収率がよい点でヒドラジン又はメチルアミンが好ましい。塩基の使用量は反応基質（１ａａ）に対して２当量以上用いて反応を実施することにより、収率よく目的物（１ａａａ）を得ることができる。本反応は溶媒中で行なうことが好ましく、反応に害を及ぼさない溶媒であれば使用することができる。溶媒としてはジエチルエーテル、ＴＨＦ、ＤＭＥ、ジオキサン等のエーテル系溶媒、アセトン、メチルエチルケトン等のケトン系溶媒、酢酸エチル、酢酸ブチル等のエステル系溶媒、アセトニトリル、プロピオニトリル等のニトリル類、ベンゼン、トルエン、キシレン、クロロベンゼン等の芳香族炭化水素系溶媒、ＤＭＦ、Ｎ−メチルピロリドン等のアミド系溶媒、メタノール、エタノール、イソプロピルアルコール等のアルコール系溶媒、ＤＭＳＯ、水又はこれらの混合溶媒が例示でき、中でも収率がよい点や後処理が簡便である点で、メタノールやエタノール等のアルコール系溶媒を用いることが好ましい。反応温度については特に制限はないが、０℃から１５０℃の範囲から適宜選ばれた温度で反応させることにより目的物を得ることができる。反応終了後は、通常の後処理操作により目的物を得ることができるが、必要であればカラムクロマトグラフィー又は再結晶等の方法により精製することもできる。

(In the formula, R ¹ , R ² , Ar and n represent the same meaning as described above.)
Production method 5 is a method of treating a thiazole derivative (1aa) with a base to produce a 5-aminoxyalkylthiazole derivative (1aaa). Bases include alkali metal bases such as sodium hydride, sodium amide, sodium carbonate, potassium carbonate, sodium methoxide, sodium ethoxide, potassium tert-butoxide, sodium hydroxide, potassium hydroxide, methylamine, ethylamine, dimethyl Organic amines such as amines and hydrazines can be used, and hydrazine or methylamine is preferred in terms of good yield. The target product (1aaa) can be obtained in good yield by carrying out the reaction using the base in an amount of 2 equivalents or more relative to the reaction substrate (1aa). This reaction is preferably carried out in a solvent, and any solvent that does not harm the reaction can be used. Solvents include ether solvents such as diethyl ether, THF, DME and dioxane, ketone solvents such as acetone and methyl ethyl ketone, ester solvents such as ethyl acetate and butyl acetate, nitriles such as acetonitrile and propionitrile, benzene and toluene. Examples include aromatic hydrocarbon solvents such as xylene and chlorobenzene, amide solvents such as DMF and N-methylpyrrolidone, alcohol solvents such as methanol, ethanol and isopropyl alcohol, DMSO, water or a mixed solvent thereof. It is preferable to use an alcohol solvent such as methanol or ethanol in terms of good yield and simple post-treatment. Although there is no restriction | limiting in particular about reaction temperature, A target object can be obtained by making it react at the temperature chosen suitably from the range of 0 to 150 degreeC. After completion of the reaction, the desired product can be obtained by ordinary post-treatment operations, but if necessary, it can be purified by a method such as column chromatography or recrystallization.

The novel thiazole derivative (1) produced in the above process is immobilized on a matrix having an activating group to which the thiazole derivative (1) can be immobilized and used for analysis or purification of proteins, particularly immunoglobulin G. Can do. The term “activating group” as used herein means that a hydroxy group, formyl group, mercapto group, carboxyl group, or aminoxy group (—ONH ₂ ) defined by R ³ in the general formula (1) is reacted in the presence of a base in some cases. A functional group that can easily form a covalent bond when it is formed, such as N-hydroxysuccinimidoxycarbonyl group, formyl group, carboxyl group, 2,2,2-trifluoroethylsulfonyloxy group, sulfonyl chloride group, tosyl Group, vinylsulfonyl group, and epoxy group. In addition, the type of functional group of the matrix activating group can be converted using a bifunctional reagent such as succinic acid dihydrazide, dimethyl pimelic acid, divinylsulfone, carbonyldiimidazole and the like. In some cases, the formed covalent bond may be treated with a reducing agent or the like.

  The material of the matrix to which the thiazole derivative (1) can be immobilized is not particularly limited. For example, a polypeptide or protein such as cross-linked albumin, agarose, alginate, carrageenan, chitin, cellulose, dextrin, dextran, starch or other polysaccharides, Synthetic polymers such as polyacrylamide, polystyrene, polyacrolein, polyvinyl alcohol, polymethacrylate, poly (2-hydroxyethyl methacrylate), perfluorocarbon, polyurethane, silica, glass, porous diatomaceous earth, alumina, zirconia, iron oxide or other Examples thereof include an inorganic compound such as a metal oxide and a matrix such as a copolymer formed by arbitrarily combining two or more of the above substances. In addition, they contain compounds such as dextran, polyethylene glycol, polyvinyl alcohol or hydrolyzed polymers such as hydrolyzed starch used in liquid phase partitioning, or compounds such as perfluorodecalin used to form emulsions. An included matrix is also included. In particular, as a matrix material in the production of the thiazole derivative-immobilized matrix of the present invention, vinyl polymers such as Toyopearl (manufactured by Tosoh Corporation), agarose, chitosan, dextran, cellulose, silica, and polystyrene are preferable.

  The vinyl polymer here refers to a polymer obtained by polymerizing a monomer having a vinyl group, and examples of the monomer include methyl methacrylate, 2-hydroxyethyl methacrylate, acrylamide, vinyl alcohol and the like. Furthermore, the vinyl polymer here includes a copolymer using two or more kinds of the above-mentioned monomers, and a polymer added with a small amount of a crosslinking agent.

  The matrix may be granular or non-particulate, soluble or insoluble in an aqueous solvent, porous or non-porous.

  The thiazole derivative of the present invention is useful for protein analysis and purification, and particularly useful for industrial purification of medical proteins, particularly immunoglobulins, by using the derivative in the form of an immobilized matrix.

The chromatographic result of the immunoglobulin using the thiazole derivative fixed HiTrap column (the result of the compound 75). The chromatography result of the immunoglobulin using the thiazole derivative fixed vinyl polymer gel (the result of the compound 95). The chromatographic result of the immunoglobulin using the thiazole derivative fixed vinyl polymer gel (result of the compound 110).

  Hereinafter, although an example and a reference example explain the present invention still in detail, the present invention is not limited to these.

  Example 1

Ｎ−［（５−オキソヘキサン−１−イル）オキシ］フタルイミド（１．０ｇ，３．８３ｍｍｏｌ）のＴＨＦ（１５ｍＬ）溶液に臭化銅（ＩＩ）（１．７１ｇ，７．６６ｍｍｏｌ）を加え、２時間加熱還流した。反応液をセライト濾過により固体を瀘別し、濾液の溶媒を減圧留去して油状物を得た。得られた油状物をＤＭＦ（２０ｍＬ）に溶解し、３，４−ジメトキシベンゾイルチオウレア（０．９２ｇ，３．８３ｍｍｏｌ）を加え、８０℃で８時間反応させた。反応終了後、溶媒を減圧留去して得られた粗生成物に１Ｍ塩酸（３０ｍＬ）と酢酸エチル（３０ｍＬ）を加え固体を析出させた。固体を瀘別し、酢酸エチルとエーテルによって充分洗浄し、灰色固体の３，４−ジメトキシ−Ｎ−［４−メチル−５−｛３−（フタルイミドオキシ）プロピル｝チアゾール−２−イル］ベンズアミド（０．５７ｇ，３１％）を得た。
ｍ．ｐ．２１１℃；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ ２．１０（ｍ，２Ｈ），２．５１（ｓ，３Ｈ），３．１３（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），３．９７（ｓ，３Ｈ），４．０４（ｓ，３Ｈ），４．２６（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），７．０２（ｄ，Ｊ＝７．５Ｈｚ，１Ｈ），７．７６から７．８８（ｍ，５Ｈ），７．９９（ｄｄ，Ｊ＝２．５Ｈｚ ａｎｄ ７．５Ｈｚ，１Ｈ）。アミドのプロトンは帰属できなかった。

To a solution of N-[(5-oxohexane-1-yl) oxy] phthalimide (1.0 g, 3.83 mmol) in THF (15 mL) was added copper (II) bromide (1.71 g, 7.66 mmol), Heated to reflux for 2 hours. The reaction solution was filtered to remove solids by Celite filtration, and the solvent of the filtrate was distilled off under reduced pressure to obtain an oil. The obtained oil was dissolved in DMF (20 mL), 3,4-dimethoxybenzoylthiourea (0.92 g, 3.83 mmol) was added, and the mixture was reacted at 80 ° C. for 8 hours. After completion of the reaction, the solvent was distilled off under reduced pressure, and 1M hydrochloric acid (30 mL) and ethyl acetate (30 mL) were added to the crude product to precipitate a solid. The solid was filtered off and washed thoroughly with ethyl acetate and ether to give 3,4-dimethoxy-N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] benzamide ( 0.57 g, 31%).
m. p. 211 H; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 2.10 (m, 2H), 2.51 (s, 3H), 3.13 (t, J = 7.5 Hz, 2H), 3.97 (s, 3H), 4.04 (s, 3H), 4.26 (t, J = 7.5 Hz, 2H), 7.02 (d, J = 7.5 Hz, 1H), 7. 76 to 7.88 (m, 5H), 7.9 (dd, J = 2.5 Hz and 7.5 Hz, 1H). The proton of the amide could not be assigned.

Example 2
In the same manner as in Example 1, 3-fluoro-N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] -4-methylbenzamide was synthesized.
Gray solid, 46%; m. p. 135 to 137 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 2.04 (tt, J = 7.5 and 7.5 Hz, 2H), 2.15 (s, 3H), 2.35 (D, J _HF = 1.8 Hz, 3H), 2.99 (t, J = 7.5 Hz, 2H), 4.22 (t, J = 7.5 Hz, 2H), 7.28 (dd, J = 7.5 and 10 Hz, 1H). 7.73 to 7.88 (m, 6H). The proton of the amide could not be assigned.

Example 3
In the same manner as in Example 1, 2-chloro-N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] benzamide was synthesized.
White solid, 39%; m. p. ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 2.03 (tt, J = 7.5 and 7.5 Hz, 2H), 2.19 (s, 3H), 2.97 (T, J = 7.5 Hz, 2H), 4.24 (t, J = 7.5 Hz, 2H), 7.32 to 7.49 (m, 3H), 7.73 to 7.87 (m, 5H). The proton of the amide could not be assigned.

Example 4
In the same manner as in Example 1, 3-chloro-N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] benzamide was synthesized.
Pale yellow solid, 53%; p. 147 from _{^{155 ℃; 1 H-NMR (}} CDCl 3, TMS, ppm): δ 2.03 (tt, J = 7.5 and 7.5Hz, 2H), 2.15 (s, 3H), 2.99 (T, J = 7.5 Hz, 2H), 4.24 (t, J = 7.5 Hz, 2H), 7.38 (dd, J = 7.5 and 7.5 Hz, 1H), 7.50 7.55 (d, J = 7.5 Hz, 1H), 7.72 to 7.9 (m, 6H). The proton of the amide could not be assigned.

Example 5
In the same manner as in Example 1, N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] -4-iodobenzamide was synthesized.
Pale yellow solid, 29%; m. p. 192 to 200 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 2.03 (tt, J = 7.5 and 7.5 Hz, 2H), 2.18 (s, 3H), 2.99 (T, J = 7.5 Hz, 2H), 4.23 (t, J = 7.5 Hz, 2H), 7.60 (d, J = 7.5 Hz, 2H), 7.74 to 7.77 ( d, J = 7.5 Hz, 2H), 7.82 to 7.87 (m, 4H). The proton of the amide could not be assigned.

Example 6
In the same manner as in Example 1, N- [4-methyl-5- {7- (phthalimidooxy) heptyl} thiazol-2-yl] -4-methylbenzamide was synthesized.
Yellow solid, 58%; m. p. 95 from _{^{99 ℃; 1 H-NMR (}} CDCl 3, TMS, ppm): δ 1.35 from 1.44 (m, 4H), 1.44 from 1.57 (m, 2H), 1.57 to 1 .70 (m, 2H), 1.74 to 1.84 (m, 2H), 2.08 (s, 3H), 2.42 (s, 3H), 2.65 (t, J = 7.5 Hz , 2H), 4.17 (t, J = 7.5 Hz, 2H), 7.26 (d, J = 7.5 Hz, 2H), 7.72 to 7.85 (m, 6H). The proton of the amide could not be assigned.

Example 7
In the same manner as in Example 1, 4-cyclopropyl-N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] benzamide was synthesized.
Brown solid; m. p. ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 0.78 to 0.85 (m, 2H), 1.07 to 1.14 (m, 2H), 1.94 to 2 .01 (m, 1H), 2.05 to 2.15 (m, 2H), 2.51 (s, 3H), 3.13 (t, J = 7.5 Hz, 2H), 4.26 (t , J = 5.0 Hz, 2H), 7.23 (d, J = 7.5 Hz, 2H), 7.74 to 7.88 (m, 4H), 8.19 (d, J = 7.5 Hz, 2H). The proton of the amide could not be assigned.

Example 8
In the same manner as in Example 1, 4-ethoxy-N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] benzamide was synthesized.
White solid; m. p. 151-154 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.46 (t, 3H), 2.10 (m, 2H), 2.51 (s, 3H), 3.12 ( t, J = 7.5 Hz, 2H), 4.13 (q, J = 7.5 Hz, 2H), 4.26 (t, J = 7.5 Hz, 2H), 7.03 (d, J = 7 .5 Hz, 2H), 7.76 to 7.88 (m, 4H), 8.27 (d, J = 7.5 Hz, 2H). The proton of the amide could not be assigned.

Example 9
In the same manner as in Example 1, 3-ethoxy-4-methoxy-N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] benzamide was synthesized.
White solid; m. p. ^{1 1} H-NMR (CDCl ₃ , TMS, ppm): δ 1.48 (t, J = 7.5 Hz, 3H), 2.06 to 2.17 (m, 2H), 2.21 (S, 3H), 3.02 (t, J = 7.5 Hz, 2H), 3.94 (s, 3H), 4.13 (q, J = 7.5 Hz, 2H), 4.26 (t , J = 7.5 Hz, 2H), 6.92 (d, 1H), 7.44 to 7.50 (m, 2H), 7.73 to 7.87 (m, 4H). The proton of the amide could not be assigned.

Example 10
In the same manner as in Example 1, 4-methyl-3-methoxy-N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] benzamide was synthesized.
White solid; m. p. ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 2.03 to 2.18 (m, 2H), 2.18 (s, 3H), 2.27 (s, 3H), 3. 02 (t, J = 7.5 Hz, 2H), 3.86 (s, 3H), 4.26 (t, J = 7.5 Hz, 2H), 7.22 (d, J = 7.5 Hz, 1H ), 7.39 (dd, J = 2.5 and 7.5 Hz, 1H), 7.48 (s, 1H), 7.73 to 7.87 (m, 4H). The proton of the amide could not be assigned.

Example 11
In the same manner as in Example 1, N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] naphthalene-1-carboxamide was synthesized.
Yellow solid, 6.3%; m. p. 150 to 152 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.83 (s, 3H), 2.01 (tt, J = 7.5 and 7.5 Hz, 2H), 2.93 (T, J = 7.5 Hz, 2H), 4.23 (t, J = 7.5 Hz, 2H), 7.43 to 7.62 (m, 3H), 7.73 to 7.81 (m, 3H), 7.83 to 7.91 (m, 3H), 8.00 (dd, J = 2.5 and 7.5 Hz, 1H), 8.44 (dd, J = 2.5 and 7.5 Hz) , 1H). The proton of the amide could not be assigned.

Example 12
In the same manner as in Example 1, N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] thiophene-2-carboxamide was synthesized.
Gray solid, 42%; m. p. 153 to 155 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 2.03 (tt, J = 7.5 and 7.5 Hz, 2H), 2.53 (s, 3H), 3.11 (T, J = 7.5 Hz, 2H), 4.24 (t, J = 7.5 Hz, 2H), 7.23 (d, J = 4.8 Hz, 1H), 7.75 to 7.80 ( m, 3H), 7.83 to 7.89 (m, 2H), 8.49 (d, J = 7.5 Hz, 1H). The proton of the amide could not be assigned.

Example 13
In the same manner as in Example 1, N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] furan-2-carboxamide was synthesized.
Gray solid, 31%; m. p. 220-223 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 2.03 (tt, J = 7.5 and 7.5 Hz, 2H), 2.53 (s, 3H), 3.11 (T, J = 7.5 Hz, 2H), 4.24 (t, J = 7.5 Hz, 2H), 6.62 to 6.67 (m, 1H), 7.73 to 7.80 (m, 4H), 7.83 to 7.89 (m, 2H). The proton of the amide could not be assigned.

  Example 14

３，４−ジメトキシ−Ｎ−［４−メチル−５−｛３−（フタルイミドオキシ）プロピル｝チアゾール−２−イル］ベンズアミド（０．４７ｇ，０．９８ｍｍｏｌ）のアセトニトリル（７．８ｍＬ）と水（０．８ｍＬ）の混合溶液に、ヘキサカルボニルモリブデン（０．２６ｇ，０．９８ｍｍｏｌ）とトリエチルアミン（２．０５ｍＬ，１４．７ｍｍｏｌ）を加え、２時間加熱還流した。反応終了後、反応混合物に１Ｍ塩酸（１００ｍＬ）を加え酢酸エチル（１００ｍＬ）で２回抽出し、有機層を合わせて飽和食塩水（３００ｍＬ）で２回洗浄した。有機層を無水硫酸マグネシウムで乾燥した後、乾燥剤を濾別し溶媒を減圧留去した。得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製）で精製することで、白色固体のＮ−｛５−（３−ヒドロキシプロピル）−４−メチルチアゾール−２−イル｝−３，４−ジメトキシ−ベンズアミド（０．２１ｇ，６３％）を得た。
白色固体；ｍ．ｐ．１３１℃；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．８４から１．９４（ｍ，２Ｈ），２．０４（ｓ，３Ｈ），２．８０（ｔ，Ｊ＝７．５Ｈｚ， ２Ｈ），３．７１（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），３．８６（ｓ，３Ｈ），３．９４（ｓ，３Ｈ），６．９０（ｄｄ，Ｊ＝２．５Ｈｚ ａｎｄ ７．５Ｈｚ，１Ｈ），７．５０から７．５３（ｍ，２Ｈ）。アミドのプロトンとヒドロキシ基のプロトンは帰属できなかった。

3,4-Dimethoxy-N- [4-methyl-5- {3- (phthalimidooxy) propyl} thiazol-2-yl] benzamide (0.47 g, 0.98 mmol) in acetonitrile (7.8 mL) and water ( To a mixed solution of 0.8 mL), hexacarbonylmolybdenum (0.26 g, 0.98 mmol) and triethylamine (2.05 mL, 14.7 mmol) were added and heated to reflux for 2 hours. After completion of the reaction, 1M hydrochloric acid (100 mL) was added to the reaction mixture, and the mixture was extracted twice with ethyl acetate (100 mL). The organic layers were combined and washed twice with saturated brine (300 mL). After drying the organic layer with anhydrous magnesium sulfate, the desiccant was filtered off and the solvent was distilled off under reduced pressure. N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} -3 as a white solid was purified by purifying the obtained mixture with an automatic medium pressure column chromatography system (manufactured by Yamazen). , 4-Dimethoxy-benzamide (0.21 g, 63%) was obtained.
White solid; m. p. ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.84 to 1.94 (m, 2H), 2.04 (s, 3H), 2.80 (t, J = 7.5 Hz) , 2H), 3.71 (t, J = 7.5 Hz, 2H), 3.86 (s, 3H), 3.94 (s, 3H), 6.90 (dd, J = 2.5 Hz and 7 .5Hz, 1H), 7.50 to 7.53 (m, 2H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 15
In the same manner as in Example 14, 3-fluoro-N- {5- (3-hydroxyphthalimidopropyl) -4-methylthiazol-2-yl} -4-methylbenzamide was synthesized.
White solid, 59%; m. p. 142 to 150 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.83 (tt, J = 7.5 and 7.5 Hz, 2H), 2.09 (s, 3H), 2.32 (D, J _HF = 1.8 Hz, 3H), 2.78 (t, J = 7.5 Hz, 2H), 3.69 (t, J = 7.5 Hz, 2H), 7.22 (dd, J = 10 and 10 Hz, 1H), 7.57 to 7.61 (m, 2H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 16
In the same manner as in Example 14, 2-chloro-N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} benzamide was synthesized.
Yellow viscous, 46%; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.82 (tt, J = 7.5 and 7.5 Hz, 2H), 2.04 (s, 3H), 2.77 (t, J = 7.5 Hz, 2H), 3.68 (t, J = 7.5 Hz, 2H), 7.34 to 7.47 (m, 3H), 7.78 (dd, J = 1.8 and 7.5 Hz, 1H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 17
In the same manner as in Example 14, 3-chloro-N- {5- (3-hydroxyphthalimidopropyl) -4-methylthiazol-2-yl} benzamide was synthesized.
White solid, 54%; p. ^{1 1} H-NMR (CDCl ₃ , TMS, ppm): δ 1.81 (tt, J = 7.5 and 7.5 Hz, 2H), 2.10 (s, 3H), 2.79 (T, J = 7.5 Hz, 2H), 3.69 (t, J = 7.5 Hz, 2H), 7.39 (dd, J = 8.0 and 8.0 Hz, 1H), 7.47 ( ddd, J = 1.8, 1.8 and 8.0 Hz, 1H), 7.82 (ddd, J = 1.8, 1.8 and 8.0 Hz, 1H), 7.93 (dd, J = 1.8 and 1.8 Hz, 1H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 18
In the same manner as in Example 14, N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} -4-iodobenzamide was synthesized.
Yellow viscous, 55%; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.86 (tt, J = 7.5 and 7.5 Hz, 2H), 2.03 (s, 3H), 2.78 (t, J = 7.5 Hz, 2H), 3.69 (t, J = 7.5 Hz, 2H), 7.62 (d, J = 7.5 Hz, 2H), 7.79 (d , J = 7.5 Hz, 2H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 19
In the same manner as in Example 14, 4-cyclopropyl-N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} benzamide was synthesized.
White solid; m. p. 118 ° C; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 0.74 to 0.81 (m, 2H), 1.03 to 1.11 (m, 2H), 1.84 to 1.97 (M, 3H), 2.11 (s, 3H), 2.81 (t, J = 7.5 Hz, 2H), 3.71 (t, J = 7.5 Hz, 2H), 7.15 (d , J = 7.5 Hz, 2H), 7.79 (d, J = 7.5 Hz, 2H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 20
In the same manner as in Example 14, N- {5- (7-hydroxyheptyl) -4-methylthiazol-2-yl} -4-methylbenzamide was synthesized.
White solid, 32%; p. 135 from _{^{145 ℃; 1 H-NMR (}} CDCl 3, TMS, ppm): δ 1.00 from 1.39 (m, 6H), 1.50 from 1.67 (m, 4H), 2.19 (s , 3H), 2.42 (s, 3H), 2.65 (t, J = 7.5 Hz, 2H), 3.62 (t, J = 7.5 Hz, 2H), 7.29 (d, J = 7.5 Hz, 2H), 7.86 (d, J = 7.5 Hz, 2H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 21
N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} -3-methoxy-4-methylbenzamide was synthesized in the same manner as in Example 14.
White solid; m. p. 124 to 127 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.82 to 1.92 (m, 5H), 2.25 (s, 3H), 2.77 (t, J = 7) .5 Hz, 2H), 3.70 (t, J = 7.5 Hz, 2H), 3.77 (s, 3H), 7.16 (d, J = 7.5 Hz, 1H), 7.39 (d , J = 7.5 Hz, 1H), 7.40 (s, 1H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 22
In the same manner as in Example 14, 4-ethoxy-N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} benzamide was synthesized.
White solid; m. p. 149 to 153 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.44 (t, J = 7.5 Hz, 3H), 1.83 to 1.94 (m, 2H), 2.06 (S, 3H), 2.80 (t, J = 7.5 Hz, 2H), 3.71 (t, J = 7.5 Hz, 2H), 4.09 (q, J = 7.5 Hz, 2H) 6.93 (d, J = 10.0 Hz, 2H), 7.88 (d, J = 10.0 Hz, 2H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 23
In the same manner as in Example 14, 3-ethoxy-N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} -4-methoxybenzamide was synthesized.
White solid; m. p. ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.45 (t, J = 7.0 Hz, 3H), 1.83 to 1.94 (m, 2H), 1.98 (S, 3H), 2.79 (t, J = 7.5 Hz, 2H), 3.70 (t, J = 6.25 Hz, 2H), 3.93 (s, 3H), 4.03 (q , J = 7.0 Hz, 2H), 6.89 (d, J = 7.5 Hz, 1H), 7.48 (s, 1H), 7.50 (d, J = 7.5 Hz, 1H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 24
In the same manner as in Example 14, N- {5- (3-hydroxyphthalimidopropyl) -4-methylthiazol-2-yl} naphthalene-2-carboxamide was synthesized.
Yellow viscous, 62%; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.74 (s, 3H), 1.81 (tt, J = 7.5 and 7.5 Hz, 2H), 2.71 (t, J = 7.5 Hz, 2H), 3.67 (t, J = 7.5 Hz, 2H), 7.43 to 7.61 (m, 3H), 7.74 (dd, J = 1.0 and 7.0 Hz, 1 H), 7.86 to 7.91 (m, 1 H), 7.99 (dd, J = 1.0 and 7.0 Hz, 1 H), 8.44 to 8. 49 (m, 1H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 25
In the same manner as in Example 14, N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} thiophene-2-carboxamide was synthesized.
Yellow viscous, 21%; m. p. ^{1 2} H-NMR (CDCl ₃ , TMS, ppm): δ 1.83 (tt, J = 7.5 and 7.5 Hz, 2H), 2.20 (s, 3H), 2.78 (T, J = 7.5 Hz, 2H), 3.69 (t, J = 7.5 Hz, 2H), 7.10 to 7.14 (m, 1H), 7.57 to 7.62 (m, 1H), 7.73 to 7.75 (m, 1H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 26
In the same manner as in Example 14, N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} furan-2-carboxamide was synthesized.
Yellow viscous, 13%; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.83 (tt, J = 7.5 and 7.5 Hz, 2H), 2.26 (s, 3H), 2.79 (t, J = 7.5 Hz, 2H), 3.68 (t, J = 7.5 Hz, 2H), 6.57 to 6.59 (m, 1H), 7.38 (dd, J = 1.0 and 3.3 Hz, 1H), 7.55 to 7.60 (m, 1H). The proton of the amide and the proton of the hydroxy group could not be assigned.

  Example 27 Synthesis of N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} -4-methyl-N-methylbenzamide

Ｎ−｛５−（３−ヒドロキシプロピル）−４−メチルチアゾール−２−イル｝−４−メチル−Ｎ−メチルベンズアミド（３００ｍｇ，１．０３ｍｍｏｌ）のＤＭＦ（５ｍＬ）溶液に、ヨウ化メチル（１７６ｍｇ，１．２４ｍｍｏｌ）と炭酸カリウム（１７１ｍｇ，１．２４ｍｍｏｌ）を加え、室温で１２時間反応させた。反応終了後、反応混合物に１Ｍ塩酸（２０ｍＬ）を加え酢酸エチル（２０ｍＬ）で２回抽出し、有機層を合わせて飽和食塩水（３０ｍＬ）で２回洗浄した。有機層を無水硫酸マグネシウムで乾燥した後、乾燥剤を濾別し溶媒を減圧留去した。得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製）で精製することで、白色固体のＮ−｛５−（３−ヒドロキシプロピル）−４−メチルチアゾール−２−イル｝−４−メチル−Ｎ−メチルベンズアミド（４６ｍｇ，１５％）を得た。
白色固体；ｍ．ｐ．１６４から１６６℃；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．７７から１．８８（ｍ，２Ｈ），２．１８（ｓ，３Ｈ），２．３９（ｓ，３Ｈ），２．７０（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），３．６５（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），３．７４（ｓ，３Ｈ），７．２２（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），８．２０（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ）。ヒドロキシ基のプロトンは帰属できなかった。

To a solution of N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} -4-methyl-N-methylbenzamide (300 mg, 1.03 mmol) in DMF (5 mL) was added methyl iodide (176 mg). , 1.24 mmol) and potassium carbonate (171 mg, 1.24 mmol) were added and reacted at room temperature for 12 hours. After completion of the reaction, 1M hydrochloric acid (20 mL) was added to the reaction mixture, and the mixture was extracted twice with ethyl acetate (20 mL). The organic layers were combined and washed twice with saturated brine (30 mL). After drying the organic layer with anhydrous magnesium sulfate, the desiccant was filtered off and the solvent was distilled off under reduced pressure. N- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} -4 as a white solid was obtained by purifying the resulting mixture with an automatic medium pressure column chromatography system (manufactured by Yamazen). -Methyl-N-methylbenzamide (46 mg, 15%) was obtained.
White solid; m. p. 164 to 166 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.77 to 1.88 (m, 2H), 2.18 (s, 3H), 2.39 (s, 3H), 2.70 (t, J = 7.5 Hz, 2H), 3.65 (t, J = 7.5 Hz, 2H), 3.74 (s, 3H), 7.22 (d, J = 7.5 Hz , 2H), 8.20 (d, J = 7.5 Hz, 2H). The proton of the hydroxy group could not be assigned.

Example 28
In the same manner as in Example 14, N- {5- (3-acetoxypropyl) -4-methylthiazol-2-yl} -4-methylbenzamide was synthesized.
Brown oil: ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.90 to 2.01 (m, 2H), 2.04 (s, 3H), 2.07 (s, 3H), 2. 42 (s, 3H), 2.78 (t, J = 7.5 Hz, 2H), 4.09 (t, J = 7.5 Hz, 2H), 7.28 (d, J = 7.5 Hz, 2H) ), 7.81 (d, J = 7.5 Hz, 2H). The proton of the amide could not be assigned.

  Example 29 Synthesis of 2,4-difluoro- {5- (3-hydroxypropyl) -4-methylthiazol-2-yl} benzamide

［５−｛３−（アセトキシ）プロピル｝チアゾール−２−イル］−２，４−ジフルオロベンズアミド（５１２ｍｇ，１．４４ｍｍｏｌ）のエタノール（５ｍＬ）溶液に、炭酸カリウム（５９７ｍｇ，４．３２ｍｍｏｌ）を加え、３０分加熱還流した。反応終了後、反応混合物に１Ｍ塩酸（２０ｍＬ）を加え酢酸エチル（２０ｍＬ）で２回抽出し、有機層を合わせて飽和食塩水（３０ｍＬ）で２回洗浄した。有機層を無水硫酸マグネシウムで乾燥した後、乾燥剤を濾別し溶媒を減圧留去した。得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製）で精製することで、黄色固体の２，４−ジフルオロ−｛５−（３−ヒドロキシプロピル）−４−メチルチアゾール−２−イル｝ベンズアミド（９４ｍｇ，２１％）を得た。
黄色油状；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．８４から１．９３（ｍ，２Ｈ），２．２５（ｓ，３Ｈ），２．８３（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），３．７１（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），６．９１から７．１０（ｍ，２Ｈ），８．１２から８．２２（ｍ，１Ｈ）。アミドのプロトンとヒドロキシ基のプロトンは帰属できなかった。

To a solution of [5- {3- (acetoxy) propyl} thiazol-2-yl] -2,4-difluorobenzamide (512 mg, 1.44 mmol) in ethanol (5 mL) was added potassium carbonate (597 mg, 4.32 mmol). The mixture was heated to reflux for 30 minutes. After completion of the reaction, 1M hydrochloric acid (20 mL) was added to the reaction mixture, and the mixture was extracted twice with ethyl acetate (20 mL). The organic layers were combined and washed twice with saturated brine (30 mL). After drying the organic layer with anhydrous magnesium sulfate, the desiccant was filtered off and the solvent was distilled off under reduced pressure. The resulting mixture was purified by an automatically set medium pressure column chromatography system (manufactured by Yamazen Co., Ltd.), whereby yellow solid 2,4-difluoro- {5- (3-hydroxypropyl) -4-methylthiazole-2- Il} benzamide (94 mg, 21%) was obtained.
Yellow _{^{oil; 1 H-NMR (CDCl 3}} , TMS, ppm): δ 1.84 from 1.93 (m, 2H), 2.25 (s, 3H), 2.83 (t, J = 7.5Hz , 2H), 3.71 (t, J = 7.5 Hz, 2H), 6.91 to 7.10 (m, 2H), 8.12 to 8.22 (m, 1H). The proton of the amide and the proton of the hydroxy group could not be assigned.

Example 30
In the same manner as in Example 14, O-ethyl-S-3- [2- (4-iodobenzoylamino) -4-methylthiazol-5-yl] propyl dithiocarbonate was synthesized.
Yellow viscous, 35%; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.39 (t, J = 7.5 Hz, 3H), 1.98 (tt, J = 7.5 and 7 .5 Hz, 2H), 2.20 (s, 3H), 2.80 (t, J = 7.5 Hz, 2H), 3.13 (t, J = 7.5 Hz, 2H), 4.61 (q , J = 7.5 Hz, 2H), 7.67 (d, J = 8.8 Hz, 2H), 7.85 (d, J = 8.8 Hz, 2H). The proton of the amide could not be assigned.

Example 31
In the same manner as in Example 14, O-ethyl-S-3- [2- (4-bromobenzoylamino) -4-methylthiazol-5-yl] propyl dithiocarbonate was synthesized.
Yellow solid, 53%; m. p. 76 to 80 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.39 (t, J = 7.5 Hz, 3H), 1.98 (tt, J = 7.5 and 7.5 Hz, 2H), 2.02 (s, 3H), 2.79 (t, J = 7.5 Hz, 2H), 3.13 (t, J = 7.5 Hz, 2H), 4.61 (q, J = 7.5 Hz, 2H), 7.59 (d, J = 7.5 Hz, 2H), 7.74 (d, J = 7.5 Hz, 2H). The proton of the amide could not be assigned.

Example 32
In the same manner as in Example 14, O-ethyl-S-3- [2- (2,4-difluorobenzoylamino) -4-methylthiazol-5-yl] propyl dithiocarbonate was synthesized.
Brown oil; ¹ H-NMR (CDCl ₃ , TMS, ppm): 1.42 (t, J = 7.5 Hz, 3H), 1.99 to 2.10 (m, 2H), 2.28 (s 3H), 2.84 (t, J = 7.5 Hz, 2H), 3.17 (t, J = 7.5 Hz, 2H), 4.65 (q, J = 7.5 Hz, 2H), 6 .92 to 7.00 (m, 1H), 7.01 to 7.12 (m, 1H), 8.19 to 8.29 (m, 1H), 9.69 (brs, 1H).

Example 33
In the same manner as in Example 14, O-ethyl-S-3- [2- (4-ethoxybenzoylamino) -4-methylthiazol-5-yl] propyl dithiocarbonate was synthesized.
Brown oil; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.39 to 1.48 (m, 6H), 1.98 to 2.09 (m, 2H), 2.16 (s, 3H), 2.82 (t, J = 7.5 Hz, 2H), 3.16 (t, J = 7.5 Hz, 2H), 4.10 (q, J = 7.5 Hz, 2H), 4. 65 (q, J = 7.5 Hz, 2H), 6.96 (d, J = 7.5 Hz, 2H), 7.90 (d, J = 7.5 Hz, 2H).

Example 34
In the same manner as in Example 14, O-ethyl-S-3- [2- (3-ethoxy-4-methoxybenzoylamino) -4-methylthiazol-5-yl] propyl dithiocarbonate was synthesized.
¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.42 to 1.51 (m, 6H), 2.01 to 2.12 (m, 2H), 2.12 (s , 3H), 2.82 (t, J = 7.5 Hz, 2H), 3.16 (t, J = 7.5 Hz, 2H), 3.94 (s, 3H), 4.11 (q, J = 7.5 Hz, 2H), 4.65 (q, J = 7.5 Hz, 2H), 6.91 (d, J = 7.5 Hz, 1H), 7.47 (dd, J = 2.5 and 7.5 Hz, 1 H), 7.49 (d, J = 2.5 Hz, 1 H). The proton of the amide could not be assigned.

  Example 35 Synthesis of O-ethyl-S-3- [2- (4-chlorobenzoylamino) -4-methylthiazol-5-yl] propyldithiocarbonate

Ｏ−エチル−Ｓ−３−（２−アミノ−４−メチルチアゾール−５−イル）プロピルジチオカーボネート（０．７０ｇ，２．５３ｍｍｏｌ）のクロロホルム（１０ｍＬ）溶液に氷冷下４−クロロベンゾイルクロリド（０．４２ｍＬ，３．２９ ｍｍｏｌ）を滴下した。次いでトリエチルアミン（０．５ｍＬ，７．９８ｍｍｏｌ）のクロロホルム（５ｍＬ）溶液を加え、一晩反応させた。反応終了後、反応混合物を１Ｍ塩酸（２０ｍＬ）で２回洗浄し、飽和食塩水（２０ｍＬ）で２回洗浄して、有機層を無水硫酸マグネシウムで乾燥した後、乾燥剤を濾別し溶媒を減圧留去した。得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製）で精製し、Ｏ−エチル−Ｓ−３−［２−（４−クロロベンゾイルアミノ）−４−メチルチアゾール−５−イル］プロピルジチオカーボネートの白色固体（０．３２９ｇ，３１％）を得た。
白色固体；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．４２（ｔ，Ｊ＝７．５Ｈｚ，３Ｈ），１．９９から２．０５（ｍ，５Ｈ），２．８１（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），３．１６（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），４．６５（ｑ，Ｊ＝７．５Ｈｚ，２Ｈ），７．４３（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），７．８３（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），１１．１８（ｂｒ ｓ，１Ｈ）。

To a solution of O-ethyl-S-3- (2-amino-4-methylthiazol-5-yl) propyldithiocarbonate (0.70 g, 2.53 mmol) in chloroform (10 mL) was added 4-chlorobenzoyl chloride ( 0.42 mL, 3.29 mmol) was added dropwise. Next, a solution of triethylamine (0.5 mL, 7.98 mmol) in chloroform (5 mL) was added and allowed to react overnight. After completion of the reaction, the reaction mixture was washed twice with 1M hydrochloric acid (20 mL) and twice with saturated brine (20 mL). The organic layer was dried over anhydrous magnesium sulfate, and then the desiccant was filtered off and the solvent was removed. Distilled under reduced pressure. The resulting mixture was purified by an automatic medium pressure column chromatography system (manufactured by Yamazen Co., Ltd.) and O-ethyl-S-3- [2- (4-chlorobenzoylamino) -4-methylthiazol-5-yl]. A white solid (0.329 g, 31%) of propyl dithiocarbonate was obtained.
¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.42 (t, J = 7.5 Hz, 3H), 1.99 to 2.05 (m, 5H), 2.81 (t , J = 7.5 Hz, 2H), 3.16 (t, J = 7.5 Hz, 2H), 4.65 (q, J = 7.5 Hz, 2H), 7.43 (d, J = 7. 5 Hz, 2H), 7.83 (d, J = 7.5 Hz, 2H), 11.18 (br s, 1H).

  Example 36 Synthesis of O-ethyl-S-3- [2- (4-methylbenzoylamino) -4-methylthiazol-5-yl] propyldithiocarbonate

Ｏ−エチル−Ｓ−５−オキシヘキシルチオカーボネート（２．０ｇ，９．０８ｍｍｏｌ）のＴＨＦ（２０ｍＬ）溶液に臭化銅（ＩＩ）（３．８５ｇ，１７．３ｍｍｏｌ）を加え１時間加熱還流したところ白色の沈澱物が生成した。沈澱物をろ過し、ろ液をエバポレーターで減圧濃縮し、黄色の油状物を得た。この油状物にエタノール（５０ｍＬ）とＮ−４−メチルベンゾイルチオウレア（１．７６ｇ，９．０８ｍｍｏｌ）を加え、８０℃で８時間反応させた。反応終了後、沈澱物をろ過し、クロロホルム（２０ｍＬ）とエタノール（２０ｍＬ）の混合溶媒で洗浄した。ろ液を合わせて減圧留去し、得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製）で精製し、褐色油状のＯ−エチル−Ｓ−３−［２−（４−メチルベンゾイルアミノ）−４−メチルチアゾール−５−イル］プロピルチオカーボネート（０．４３６ｇ，１２％）を得た。
褐色油状物；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：１．４２（ｔ，Ｊ＝７．５Ｈｚ，３Ｈ），２．０１から２．０９（ｍ，５Ｈ），２．４２（ｓ，３Ｈ），２．８１（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），３．１６（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），４．６５（ｑ，Ｊ＝７．５Ｈｚ，２Ｈ），７．２６（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），７．７９（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ）。アミドのプロトンは帰属できなかった。

Copper (II) bromide (3.85 g, 17.3 mmol) was added to a THF (20 mL) solution of O-ethyl-S-5-oxyhexylthiocarbonate (2.0 g, 9.08 mmol), and the mixture was heated to reflux for 1 hour. A white precipitate was formed. The precipitate was filtered, and the filtrate was concentrated under reduced pressure using an evaporator to obtain a yellow oil. Ethanol (50 mL) and N-4-methylbenzoylthiourea (1.76 g, 9.08 mmol) were added to the oil, and the mixture was reacted at 80 ° C. for 8 hours. After completion of the reaction, the precipitate was filtered and washed with a mixed solvent of chloroform (20 mL) and ethanol (20 mL). The filtrates were combined and evaporated under reduced pressure, and the resulting mixture was purified by an automatic medium pressure column chromatography system (manufactured by Yamazen Co., Ltd.) to give brown oily O-ethyl-S-3- [2- (4-methyl). Benzoylamino) -4-methylthiazol-5-yl] propylthiocarbonate (0.436 g, 12%) was obtained.
Brown oil; ¹ H-NMR (CDCl ₃ , TMS, ppm): 1.42 (t, J = 7.5 Hz, 3H), 2.01 to 2.09 (m, 5H), 2.42 (s 3H), 2.81 (t, J = 7.5 Hz, 2H), 3.16 (t, J = 7.5 Hz, 2H), 4.65 (q, J = 7.5 Hz, 2H), 7 .26 (d, J = 7.5 Hz, 2H), 7.79 (d, J = 7.5 Hz, 2H). The proton of the amide could not be assigned.

  Example 37 Synthesis of S-3- (2-amino-4-methylthiazol-5-yl) -O-ethylpropyl dithiocarbonate

Ｏ−エチル−Ｓ−５−オキシヘキシルチオカーボネート（４．０ｇ，１８．２ｍｍｏｌ）のＴＨＦ（４０ｍＬ）溶液に臭化銅（ＩＩ）（７．７０ｇ，３４．６ｍｍｏｌ）を加え１．５時間加熱還流したところ白色の沈澱物が生成した。沈澱物をろ過し、ろ液をエバポレーターで減圧濃縮し、黄色の油状物を得た。この油状物にエタノール（４０ｍＬ）とチオウレア（１．３８ｇ，１８．２ｍｍｏｌ）を加え、８０℃で８時間反応させた。反応終了後、溶媒を減圧留去し，反応混合物に１Ｍ塩酸（３０ｍＬ）を加え酢酸エチル（５０ｍＬ）で２回抽出し、有機層を合わせて飽和食塩水（３０ｍＬ）で２回洗浄した。有機層を無水硫酸マグネシウムで乾燥した後、乾燥剤を濾別し溶媒を減圧留去した。得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製）で精製し、得られた白色固体を少量の酢酸エチル／エタノールの混合溶媒で洗浄することによって、白色固体のＯ−エチル−Ｓ−３−（２−アミノ−４−メチルチアゾール−５−イル）プロピルジチオカーボネート（１．５６ｇ，３１％）を得た。
^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．４３（ｔ，Ｊ＝７．５Ｈｚ，３Ｈ），１．８２から２．０２（ｍ，２Ｈ），２．２４（ｓ，３Ｈ），２．６８（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），３．１５（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），４．６５（ｑ，Ｊ＝７．５Ｈｚ，２Ｈ），８．６５（ｂｒ ｓ，２Ｈ）。

Copper (II) bromide (7.70 g, 34.6 mmol) was added to a THF (40 mL) solution of O-ethyl-S-5-oxyhexylthiocarbonate (4.0 g, 18.2 mmol) and heated for 1.5 hours. Upon reflux, a white precipitate was formed. The precipitate was filtered, and the filtrate was concentrated under reduced pressure using an evaporator to obtain a yellow oil. Ethanol (40 mL) and thiourea (1.38 g, 18.2 mmol) were added to this oily substance and reacted at 80 ° C. for 8 hours. After completion of the reaction, the solvent was distilled off under reduced pressure, 1M hydrochloric acid (30 mL) was added to the reaction mixture, and the mixture was extracted twice with ethyl acetate (50 mL). The organic layers were combined and washed twice with saturated brine (30 mL). After drying the organic layer with anhydrous magnesium sulfate, the desiccant was filtered off and the solvent was distilled off under reduced pressure. The obtained mixture was purified by an automatic medium pressure column chromatography system (manufactured by Yamazen Co., Ltd.), and the obtained white solid was washed with a small amount of a mixed solvent of ethyl acetate / ethanol to obtain a white solid O-ethyl- S-3- (2-amino-4-methylthiazol-5-yl) propyl dithiocarbonate (1.56 g, 31%) was obtained.
¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.43 (t, J = 7.5 Hz, 3H), 1.82 to 2.02 (m, 2H), 2.24 (s, 3H) , 2.68 (t, J = 7.5 Hz, 2H), 3.15 (t, J = 7.5 Hz, 2H), 4.65 (q, J = 7.5 Hz, 2H), 8.65 ( br s, 2H).

  Example 38 Synthesis of 4-chloro-N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] benzamide

Ｏ−エチル−Ｓ−３−［２−（４−クロロベンゾイルアミノ）−４−メチルチアゾール−５−イル］プロピルジチオカーボネート（０．０８ｇ，０．２４１ｍｍｏｌ）のエタノール（１ｍＬ）溶液に２８％アンモニア水（０．８ｍＬ）を加え常温で一晩反応させた。反応終了後、４Ｍ塩酸（１０ｍＬ）を加え、酢酸エチル（２０ｍＬ）で抽出し、有機相を飽和食塩水（２０ｍＬ）で洗浄した。有機層を無水硫酸マグネシウムで乾燥した後、乾燥剤を濾別し溶媒を減圧留去した。得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製）で精製し、４−クロロ−Ｎ−［５−（３−メルカプトプロピル）−４−メチルチアゾール−２−イル］ベンズアミドの白色固体（０．０４３ｇ，５５％）を得た。
白色固体；ｍ．ｐ．１８０から１８３℃；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：１．９９から２．０５（ｍ，５Ｈ），２．７０（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），２．８０（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），７．４１（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），７．８２（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），１０．８（ｂｒ ｓ，１Ｈ）。メルカプト基のプロトンは帰属できなかった。

28% ammonia in a solution of O-ethyl-S-3- [2- (4-chlorobenzoylamino) -4-methylthiazol-5-yl] propyldithiocarbonate (0.08 g, 0.241 mmol) in ethanol (1 mL). Water (0.8 mL) was added and reacted at room temperature overnight. After completion of the reaction, 4M hydrochloric acid (10 mL) was added, extracted with ethyl acetate (20 mL), and the organic phase was washed with saturated brine (20 mL). After drying the organic layer with anhydrous magnesium sulfate, the desiccant was filtered off and the solvent was distilled off under reduced pressure. The resulting mixture was purified by an automatic medium pressure column chromatography system (manufactured by Yamazen Co., Ltd.) and white of 4-chloro-N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] benzamide. A solid (0.043 g, 55%) was obtained.
White solid; m. p. 180 to 183 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): 1.99 to 2.05 (m, 5H), 2.70 (t, J = 7.5 Hz, 2H), 2.80 ( t, J = 7.5 Hz, 2H), 7.41 (d, J = 7.5 Hz, 2H), 7.82 (d, J = 7.5 Hz, 2H), 10.8 (brs, 1H) . The proton of the mercapto group could not be assigned.

Example 39
In the same manner as in Example 38, 2,4-difluoro-N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] benzamide was synthesized.
Brown solid; m. p. 151 to 155 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.98 to 2.14 (m, 2H), 2.26 (s, 3H), 2.72 (t, J = 7 .5 Hz, 2H), 2.84 (t, J = 7.5 Hz, 2H), 6.91 to 7.00 (m, 1H), 7.03 to 7.11 (m, 1H), 8.15 To 8.27 (m, 1 H), 9.88 (br s, 1 H). The proton of the mercapto group could not be assigned.

Example 40
In the same manner as in Example 38, 4-ethoxy-N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] benzamide was synthesized.
Brown solid; m. p. 180 to 183 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.44 (t, J = 7.5 Hz, 3H), 2.07 to 2.14 (m, 2H), 2.17 (S, 3H), 2.70 (t, J = 7.5 Hz, 2H), 2.80 (t, J = 7.5 Hz, 2H), 4.09 (q, J = 7.5 Hz, 2H) 6.93 (d, J = 7.5 Hz, 2H), 7.87 (d, J = 7.5 Hz, 2H). The protons of amide and mercapto groups could not be assigned.

Example 41
In the same manner as in Example 38, 3-ethoxy-N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] -4-methoxybenzamide was synthesized.
White solid; m. p. 89 to 94 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.48 (t, J = 7.5 Hz, 3H), 1.99 to 2.10 (m, 2H), 2.16 (S, 3H), 2.71 (t, J = 7.5 Hz, 2H), 2.81 (t, J = 7.5 Hz, 2H), 3.93 (s, 3H), 4.12 (q , J = 7.5 Hz, 2H), 6.90 (d, J = 7.5 Hz, 1H), 7.45 (dd, J = 1.0 and 7.5 Hz, 1H), 7.49 (d, J = 1.0 Hz, 1H), 10.1 (brs, 1H). The proton of the mercapto group could not be assigned.

  Example 42 Synthesis of N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] -4-methylbenzamide

Ｏ−エチル−Ｓ−３−［２−（４−メチルベンゾイルアミノ）−４−メチルチアゾール−５−イル］プロピルジチオカーボネート（０．４１６ｇ，１．０５ｍｍｏｌ）のエタノール（４ｍＬ）溶液に２８％アンモニア水（１ｍＬ）を加え常温で一晩反応させた。反応終了後、４Ｍ塩酸（１０ｍＬ）を加え、酢酸エチル（２０ｍＬ）で抽出し、有機相を飽和食塩水（２０ｍＬ）で洗浄した。有機層を無水硫酸マグネシウムで乾燥した後、乾燥剤を濾別し溶媒を減圧留去した。得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製）で精製し、Ｎ−［５−（３−メルカプトプロピル）−４−メチルチアゾール−２−イル］−４−メチルベンズアミドの白色固体（０．１４３ｇ，４５％）を得た。
白色固体；ｍ．ｐ．１８０から１８３℃；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．９６から２．１４（ｍ，５Ｈ），２．４２（ｓ，３Ｈ），２．７０（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），２．８０（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），７．２８（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），７．７９（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），１０．２（ｂｒ ｓ，１Ｈ）。メルカプト基のプロトンは帰属できなかった。

28% ammonia in a solution of O-ethyl-S-3- [2- (4-methylbenzoylamino) -4-methylthiazol-5-yl] propyldithiocarbonate (0.416 g, 1.05 mmol) in ethanol (4 mL). Water (1 mL) was added and reacted at room temperature overnight. After completion of the reaction, 4M hydrochloric acid (10 mL) was added, extracted with ethyl acetate (20 mL), and the organic phase was washed with saturated brine (20 mL). After drying the organic layer with anhydrous magnesium sulfate, the desiccant was filtered off and the solvent was distilled off under reduced pressure. The resulting mixture was purified by an automatic medium pressure column chromatography system (manufactured by Yamazen Co., Ltd.) and white of N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] -4-methylbenzamide. A solid (0.143 g, 45%) was obtained.
White solid; m. p. 180 to 183 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.96 to 2.14 (m, 5H), 2.42 (s, 3H), 2.70 (t, J = 7 .5 Hz, 2H), 2.80 (t, J = 7.5 Hz, 2H), 7.28 (d, J = 7.5 Hz, 2H), 7.79 (d, J = 7.5 Hz, 2H) , 10.2 (br s, 1H). The proton of the mercapto group could not be assigned.

  Example 43 Synthesis of 4-iodo-N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] benzamide

Ｏ−エチル−Ｓ−３−［２−（４−ヨードベンゾイルアミノ）−４−メチルチアゾール−５−イル］プロピルジチオカーボネート（０．２５ｇ，０．４９ｍｍｏｌ）のエタノール（２ｍＬ）懸濁液に２８％アンモニア水（１ｍＬ）を加え、室温で一晩撹拌した。反応終了後、反応液に１Ｎ塩酸（１０ｍＬ）と酢酸エチル（１０ｍＬ）を加えて抽出し、有機層を水（１０ｍＬ）で洗浄した。得られた有機層に硫酸マグネシウムを加えて乾燥させた後、乾燥剤を濾別し溶媒を減圧留去した。得られた残渣をシリカゲルカラムクロマトグラフィーで精製することにより、４−ヨード−Ｎ−［５−（３−メルカプトプロピル）−４−メチルチアゾール−２−イル］ベンズアミドの淡黄色固体（７１％，０．１６ｇ）を得た。
ｍ．ｐ．１３０から１４０℃；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．９９（ｔｔ，Ｊ＝７．５ ａｎｄ ７．５Ｈｚ，２Ｈ），２．０６（ｓ，３Ｈ），２．６７（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），２．７８（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），７．５７（ｄ，Ｊ＝８．８Ｈｚ，２Ｈ），７．８０（ｄ，Ｊ＝８．８Ｈｚ，２Ｈ）。メルカプト基のプロトンとアミドのプロトンは帰属できなかった。

28 To a suspension of O-ethyl-S-3- [2- (4-iodobenzoylamino) -4-methylthiazol-5-yl] propyl dithiocarbonate (0.25 g, 0.49 mmol) in ethanol (2 mL) % Aqueous ammonia (1 mL) was added, and the mixture was stirred overnight at room temperature. After completion of the reaction, 1N hydrochloric acid (10 mL) and ethyl acetate (10 mL) were added to the reaction solution for extraction, and the organic layer was washed with water (10 mL). Magnesium sulfate was added to the obtained organic layer for drying, and then the desiccant was filtered off and the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography to give 4-iodo-N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] benzamide as a pale yellow solid (71%, 0 .16 g) was obtained.
m. p. 130 to 140 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.99 (tt, J = 7.5 and 7.5 Hz, 2H), 2.06 (s, 3H), 2.67 (T, J = 7.5 Hz, 2H), 2.78 (t, J = 7.5 Hz, 2H), 7.57 (d, J = 8.8 Hz, 2H), 7.80 (d, J = 8.8 Hz, 2H). Mercapto group protons and amide protons could not be assigned.

  Example 44 Synthesis of 4-bromo-N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] benzamide

Ｏ−エチル−Ｓ−３−［２−（４−ブロモベンゾイルアミノ）−４−メチルチアゾール−５−イル］プロピルジチオカーボネート（０．３０ｇ，０．６５ｍｍｏｌ）のエタノール（２ｍＬ）懸濁液に２８％アンモニア水（１ｍＬ）を加え、室温で一晩撹拌した。反応終了後、反応液に１Ｎ塩酸（１０ｍＬ）と酢酸エチル（１０ｍＬ）を加えて抽出し、有機層を水（１０ｍＬ）で洗浄した。得られた有機層に硫酸マグネシウムを加えて乾燥させた後、乾燥剤を濾別し溶媒を減圧留去した。得られた残渣をシリカゲルカラムクロマトグラフィーで精製することにより、４−ブロモ−Ｎ−［５−（３−メルカプトプロピル）−４−メチルチアゾール−２−イル］ベンズアミドの白色固体（６０％，０．１５ｇ）を得た。
ｍ．ｐ．９９から１０５℃；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．９６（ｔｔ，Ｊ＝７．５ ａｎｄ ７．５Ｈｚ，２Ｈ），２．１０（ｓ，３Ｈ），２．６７（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），２．７８（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），７．５９（ｄ，Ｊ＝８．８Ｈｚ，２Ｈ），７．７４（ｄ，Ｊ＝８．８Ｈｚ，２Ｈ）。メルカプト基のプロトンとアミドのプロトンは帰属できなかった。

28 To a suspension of O-ethyl-S-3- [2- (4-bromobenzoylamino) -4-methylthiazol-5-yl] propyl dithiocarbonate (0.30 g, 0.65 mmol) in ethanol (2 mL) % Aqueous ammonia (1 mL) was added, and the mixture was stirred overnight at room temperature. After completion of the reaction, 1N hydrochloric acid (10 mL) and ethyl acetate (10 mL) were added to the reaction solution for extraction, and the organic layer was washed with water (10 mL). Magnesium sulfate was added to the obtained organic layer for drying, and then the desiccant was filtered off and the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography to give 4-bromo-N- [5- (3-mercaptopropyl) -4-methylthiazol-2-yl] benzamide as a white solid (60%, .0. 15 g) was obtained.
m. p. 99 to 105 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.96 (tt, J = 7.5 and 7.5 Hz, 2H), 2.10 (s, 3H), 2.67 (T, J = 7.5 Hz, 2H), 2.78 (t, J = 7.5 Hz, 2H), 7.59 (d, J = 8.8 Hz, 2H), 7.74 (d, J = 8.8 Hz, 2H). Mercapto group protons and amide protons could not be assigned.

  Example 45 Synthesis of 4-cyclopropyl-N- {4-methyl-5- (2-formylethyl) thiazol-2-yl} -N-methylthiomethylbenzamide

二塩化オキサリル（４１６ｍｇ，３．２８ｍｍｏｌ）の塩化メチレン（５ｍＬ）溶液にＤＭＳＯ（１７６ｍｇ，１．２４ｍｍｏｌ）の塩化メチレン（５ｍＬ）溶液を加え、−７８℃で３０分間撹拌した後、４−シクロプロピル−Ｎ−｛４−メチル−５−（３−ヒドロキシプロピル）チアゾール−２−イル｝ベンズアミド（８２８ｍｇ，２．６２ｍｍｏｌ）のクロロホルム（２０ｍＬ）溶液を滴下した。３０分間撹拌した後に、トリエチルアミンを（１．４６ｇ，１４．４ｍｍｏｌ）を加えて−７８℃で３時間反応させた。反応終了後、反応混合物に１Ｍ塩酸（４０ｍＬ）を加え、クロロホルム（１０ｍＬ）で２回抽出し、有機層を合わせて飽和食塩水（３０ｍＬ）で２回洗浄した。有機層を無水硫酸マグネシウムで乾燥した後、乾燥剤を濾別し溶媒を減圧留去した。得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製）で精製することで、黄色油状の４−シクロプロピル−Ｎ−｛４−メチル−５−（２−ホルミルエチル）チアゾール−２−イル｝ベンズアミド（２５１ｍｇ，３９％）と黄色油状の４−シクロプロピル−Ｎ−｛４−メチル−５−（２−ホルミルエチル）チアゾール−２−イル｝−Ｎ−メチルチオメチルベンズアミド（３８６ｍｇ，３０％）を得た。

To a solution of oxalyl dichloride (416 mg, 3.28 mmol) in methylene chloride (5 mL) was added a solution of DMSO (176 mg, 1.24 mmol) in methylene chloride (5 mL), stirred at −78 ° C. for 30 minutes, and then 4-cyclopropyl A solution of -N- {4-methyl-5- (3-hydroxypropyl) thiazol-2-yl} benzamide (828 mg, 2.62 mmol) in chloroform (20 mL) was added dropwise. After stirring for 30 minutes, triethylamine (1.46 g, 14.4 mmol) was added and reacted at −78 ° C. for 3 hours. After completion of the reaction, 1M hydrochloric acid (40 mL) was added to the reaction mixture, extracted twice with chloroform (10 mL), and the organic layers were combined and washed twice with saturated brine (30 mL). After drying the organic layer with anhydrous magnesium sulfate, the desiccant was filtered off and the solvent was distilled off under reduced pressure. The obtained mixture was purified by an automatic medium pressure column chromatography system (manufactured by Yamazen Co., Ltd.) to give 4-cyclopropyl-N- {4-methyl-5- (2-formylethyl) thiazole-2 as a yellow oil. -Il} benzamide (251 mg, 39%) and 4-cyclopropyl-N- {4-methyl-5- (2-formylethyl) thiazol-2-yl} -N-methylthiomethylbenzamide (386 mg, 30%) as a yellow oil %).

4-cyclopropyl-N- {4-methyl-5- (2-formylethyl) thiazol-2-yl} benzamide; yellow oil; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 0.74 to 0 .80 (m, 2H), 1.01 to 1.08 (m, 2H), 1.91 to 1.98 (m, 4H), 2.78 (t, J = 7.5 Hz, 2H), 3 .01 (t, J = 7.5 Hz, 2H), 7.11 (d, J = 10.0 Hz, 2H), 7.78 (d, J = 10.0 Hz, 2H), 9.82 (s, 1H). The proton of the amide could not be assigned.

4-cyclopropyl-N-methylthiomethyl-N- {4-methyl-5- (2-formylethyl) thiazol-2-yl} benzamide; yellow oil: ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 0.72 to 0.78 (m, 2H), 1.00 to 1.08 (m, 2H), 1.88 to 1.96 (m, 1H), 2.14 (s, 3H), 2. 30 (s, 3H), 2.78 (t, J = 7.5 Hz, 2H), 3.01 (t, J = 7.5 Hz, 2H), 5.24 (s, 2H), 7.12 ( d, J = 7.5 Hz, 2H), 7.44 (d, J = 7.5 Hz, 2H), 9.81 (s, 1H).

Example 46
In the same manner as in Example 45, 4-methoxy-3-ethoxy- {4-methyl-5- (2-formylethyl) thiazol-2-yl} benzamide was synthesized.
Yellow _{^{oil; 1 H-NMR (CDCl 3}} , TMS, ppm): δ 1.46 (t, J = 5.0Hz, 3H), 2.05 (s, 3H), 2.80 (t, J = 7 .5 Hz, 2H), 3.01 (t, J = 7.5 Hz, 2H), 3.94 (s, 3H), 4.08 (q, J = 7.5 Hz, 2H), 6.91 (d , J = 7.5 Hz, 1H), 7.49 (d, J = 7.5 Hz, 1H), 7.50 (s, 1H), 9.83 (s, 1H). The proton of the amide could not be assigned.

Example 47
In the same manner as in Example 45, 4-methoxy-3-ethoxy-N-methylthiomethyl- {4-methyl-5- (2-formylethyl) thiazol-2-yl} benzamide was synthesized.
White solid; m. p. 103 ° C; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.47 (t, J = 7.5 Hz, 3H), 2.20 (s, 3H), 2.31 (s, 3H), 2.78 (t, J = 7.5 Hz, 2H), 3.01 (t, J = 7.5 Hz, 2H), 3.92 (s, 3H), 4.11 (q, J = 7.5 Hz) , 2H), 5.26 (s, 2H), 6.90 (d, J = 7.5 Hz, 1H), 7.11 to 7.18 (m, 2H), 9.81 (s, 1H).

Example 48
In the same manner as in Example 45, 4-ethoxy- {4-methyl-5- (2-formylethyl) thiazol-2-yl} benzamide was synthesized.
Yellow solid; m. p. 110 to 114 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.44 (t, J = 7.5 Hz, 3H), 2.03 (s, 3H), 2.80 (t, J = 7.5 Hz, 2H), 3.00 (t, J = 7.5 Hz, 2H), 4.10 (q, J = 7.5 Hz, 2H), 6.93 (d, J = 10.0 Hz, 2H), 7.91 (d, J = 10.0 Hz, 2H), 9.82 (s, 1H). The proton of the amide could not be assigned.

Example 49
In the same manner as in Example 45, 4-methyl- {4-methyl-5- (2-formylethyl) thiazol-2-yl} benzamide was synthesized.
White solid; m. p. ¹ ₃ H-NMR (CDCl ₃ , TMS, ppm): δ 2.05 (s, 3H), 2.43 (s, 3H), 2.80 (t, J = 7.5 Hz, 2H) ), 3.02 (t, J = 7.5 Hz, 2H), 7.28 (d, J = 7.5 Hz, 2H), 7.79 (d, J = 7.5 Hz, 2H), 9.83 (S, 1H). The proton of the amide could not be assigned.

Example 50
In the same manner as in Example 45, 4-methyl-N-methylthiomethyl- {4-methyl-5- (2-formylethyl) thiazol-2-yl} benzamide was synthesized.
White solid; m. p. 93 to 99 ° C .; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 2.14 (s, 3H), 2.30 (s, 3H), 2.40 (s, 3H), 2.78 ( t, J = 7.5 Hz, 2H), 3.02 (t, J = 7.5 Hz, 2H), 5.24 (s, 2H), 7.25 (d, J = 7.5 Hz, 2H), 7.44 (d, J = 7.5 Hz, 2H), 9.81 (s, 1H).

  Example 51 Synthesis of N- [5- (3-aminoxypropyl) -4-methylthiazol-2-yl] -4-methylbenzamide

Ｎ−［５−（３−フタルイミドオキシプロピル）−４−メチルチアゾール−２−イル］４−メチルベンズアミド（１．７０ｇ、３．９０ｍｍｏｌ）のエタノール（２０ｍＬ）溶液に、ヒドラジン一水和物（０．４５ｍＬ、８９．９ｍｍｏｌ）を室温にて加え、一晩撹拌した。反応終了後、反応溶液を減圧留去し、得られた混合物を自動設定中圧カラムクロマトグラフィーシステム（山善社製；アミノプロピルシリカゲルを使用）で精製し、Ｎ−［５−（３−アミノキシプロピル）−４−メチルチアゾール−２−イル］−４−メチルベンズアミドの淡黄色油状物（０．９７５ｇ，収率：８２％）を得た。
淡黄色油状物；^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．８４から１．９９（ｍ，２Ｈ），２．１２（ｓ，３Ｈ），２．４３（ｓ，３Ｈ），２．７６（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），３．７０（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），５．２８（ｂｒ ｓ，２Ｈ），７．２８（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），７．８０（ｄ，Ｊ＝７．５Ｈｚ，２Ｈ），１０．１（ｂｒ ｓ，１Ｈ）。

To a solution of N- [5- (3-phthalimidooxypropyl) -4-methylthiazol-2-yl] 4-methylbenzamide (1.70 g, 3.90 mmol) in ethanol (20 mL) was added hydrazine monohydrate (0 .45 mL, 89.9 mmol) was added at room temperature and stirred overnight. After completion of the reaction, the reaction solution was distilled off under reduced pressure, and the resulting mixture was purified with an automatic medium pressure column chromatography system (manufactured by Yamazen; using aminopropyl silica gel), and N- [5- (3-aminoxy A pale yellow oil (0.975 g, yield: 82%) of propyl) -4-methylthiazol-2-yl] -4-methylbenzamide was obtained.
Pale yellow oil; ¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.84 to 1.99 (m, 2H), 2.12 (s, 3H), 2.43 (s, 3H), 2.76 (t, J = 7.5 Hz, 2H), 3.70 (t, J = 7.5 Hz, 2H), 5.28 (brs, 2H), 7.28 (d, J = 7. 5 Hz, 2H), 7.80 (d, J = 7.5 Hz, 2H), 10.1 (brs, 1H).

  Example 52 Synthesis of 8- (2-toluylamino-4-methylthiazol-5-yl) octenoic acid

１０−オキソウンデカン酸（１．０ｇ，４．９９ｍｍｏｌ）のＴＨＦ（５０ｍＬ）溶液に臭化銅（ＩＩ）（２．２３ｇ，９．９８ｍｍｏｌ）を加え、３時間加熱還流したところ白色の沈澱物が生成した。沈澱物をろ過し、ろ液をエバポレーターで減圧濃縮し、黄色の油状物を得た。この油状物にＤＭＦ（２０ｍＬ）と４−メチルベンゾイルチオウレア（０．７７５ｇ，３．９９ｍｍｏｌ）を加え、８０℃で１２時間反応させた。反応終了後反応混合物に１Ｍ塩酸（１００ｍＬ）を加え酢酸エチル（１００ｍＬ）で２回抽出し、有機層を合わせて水（２００ｍＬ）で２回洗浄した。有機層を無水硫酸マグネシウムで乾燥した後、乾燥剤を濾別し溶媒を減圧留去し、固体を析出させた。得られた固体をエーテルで洗浄することによって、白色固体の８−［２−（４−メチルベンゾイル）アミノ−４−メチルチアゾール−５−イル］オクテン酸（０．２８５ｇ，１５％）を得た。
^１Ｈ−ＮＭＲ（ＣＤＣｌ_３，ＴＭＳ，ｐｐｍ）：δ １．３０から１．３８（ｍ，６Ｈ），１．５８から１．６４（ｍ，４Ｈ），２．２２（ｓ，３Ｈ），２．３０（ｔ，Ｊ＝７．５Ｈｚ，２Ｈ），２．４２（ｓ，３Ｈ），２．６７（ｔ，Ｊ＝７．５Ｈｚ，３Ｈ），７．２９（ｄ，Ｊ＝８．０Ｈｚ，２Ｈ），７．９７（ｄ，Ｊ＝８．０Ｈｚ，２Ｈ）。アミドのプロトンとカルボン酸のプロトンは帰属できなかった。

Copper (II) bromide (2.23 g, 9.98 mmol) was added to a solution of 10-oxoundecanoic acid (1.0 g, 4.99 mmol) in THF (50 mL) and heated to reflux for 3 hours. As a result, a white precipitate was obtained. Generated. The precipitate was filtered, and the filtrate was concentrated under reduced pressure using an evaporator to obtain a yellow oil. To this oil was added DMF (20 mL) and 4-methylbenzoylthiourea (0.775 g, 3.99 mmol), and the mixture was reacted at 80 ° C. for 12 hours. After completion of the reaction, 1M hydrochloric acid (100 mL) was added to the reaction mixture, and the mixture was extracted twice with ethyl acetate (100 mL). The organic layers were combined and washed twice with water (200 mL). After drying the organic layer over anhydrous magnesium sulfate, the desiccant was filtered off and the solvent was distilled off under reduced pressure to precipitate a solid. The obtained solid was washed with ether to obtain white solid 8- [2- (4-methylbenzoyl) amino-4-methylthiazol-5-yl] octenoic acid (0.285 g, 15%). .
¹ H-NMR (CDCl ₃ , TMS, ppm): δ 1.30 to 1.38 (m, 6H), 1.58 to 1.64 (m, 4H), 2.22 (s, 3H), 2 .30 (t, J = 7.5 Hz, 2H), 2.42 (s, 3H), 2.67 (t, J = 7.5 Hz, 3H), 7.29 (d, J = 8.0 Hz, 2H), 7.97 (d, J = 8.0 Hz, 2H). The amide proton and the carboxylic acid proton could not be assigned.

Example 53
In the same manner as in Example 14, N- [5- (3-hydroxypropyl) -4-methylthiazol-2-yl] -4-methylbenzamide was synthesized.
Gray solid; m. p. ¹ ₃ H-NMR (CDCl ₃ , TMS, ppm): 1.83 to 1.95 (m, 2H), 2.10 (s, 3H), 2.42 (s, 3H), 2 .81 (t, J = 7.5 Hz, 2H), 3.71 (t, J = 7.5 Hz, 2H), 7.27 (d, J = 7.5 Hz, 2H), 7.80 (d, J = 7.5 Hz, 2H). The proton of the amide and the proton of the hydroxy group could not be assigned.

  The thiazole derivatives listed in Tables 1 to 10 were obtained according to the aforementioned synthesis method.

以下の参考例では、本発明のチアゾール誘導体を活性化基含有マトリックスに固定化し、免疫グロブリンの分析又は精製を行なった。なお、化合物番号は表１から１０記載の化合物番号に対応する。

In the following Reference Examples, the thiazole derivative of the present invention was immobilized on an activation group-containing matrix, and immunoglobulin analysis or purification was performed. The compound numbers correspond to the compound numbers described in Tables 1 to 10.

The amount of thiazole derivative immobilized was determined by measuring the amount of thiazole derivative in the solution used for the immobilization reaction and the amount of thiazole derivative in the solution recovered after the immobilization reaction (including the washing liquid, hereinafter referred to as the recovered liquid) Calculated by comparison. The concentration of the thiazole derivative is measured by measuring the absorbance at the maximum ultraviolet absorption wavelength of the derivative using a U-2900 spectrophotometer (trade name) (manufactured by Hitachi, Ltd.) (hereinafter referred to as the absorbance method), or by high performance liquid chromatography. Was used to determine the peak area in the ultraviolet absorption chromatogram of the derivative (hereinafter referred to as HPLC method). The HPLC _{method, TSK-GEL ODS-80T M} ( trade name) as a column using a (Tosoh Corp.), acetonitrile / water / trifluoroacetic acid = 45/55 / 0.05 as eluent (volume ratio) (hereinafter, Simply referred to as eluent).

Reference Example 1 Immobilization of Compound 75 on Agarose Gel (Part 1)
HiTrap NHS-activated HP column (1 mL) (trade name) (trade name, manufactured by GE Healthcare Biosciences), agarose gel activated with N-hydroxysuccinimide (hereinafter referred to as NHS): 10 μmol / mL (Gel), hereinafter referred to as HiTrap column) was used as an activation group-containing matrix packed column.

  First, 5 mL of ice-cold 1 mM HCl was passed through the HiTrap column. Succinic acid dihydrazide was dissolved in 0.2 M sodium carbonate buffer (pH 8.3) containing 0.5 M NaCl to a concentration of 100 mM, and 2 mL thereof was passed through a HiTrap column. After standing for 1 hour, 0.2 M sodium carbonate buffer (pH 8.3) containing 10 mL of 0.5 M NaCl was passed through. Next, after passing 10 mL of pure water, 5 mL of DMSO was passed. Next, Compound 75 was dissolved in DMSO to a concentration of 10 mM, and 2 mL thereof was passed through. After standing for 2 hours, 3 mL of DMSO was passed through. Next, 2 mL of a pyridine-borane solution in DMSO (20 mM) was passed through, allowed to stand for 14 hours, and then 5 mL of DMSO was passed through. Finally, 10 mL of pure water was passed through the column to obtain a gel in which compound 75 was immobilized.

  The amount of compound 75 immobilized was calculated by subtracting the amount of compound 75 recovered from the column from the amount of compound 75 added to the column (20 μmol). The amount of compound 75 immobilized measured by the absorbance method (detection wavelength 306 nm) was 7.3 μmol / mL (gel).

Reference Example 2 Absorption and Desorption of Immunoglobulin Using Agarose Gel Immobilized with Compound 75 The HiTrap column with Compound 75 immobilized, prepared by the method described in Reference Example 1, was used as a chromatographic apparatus, AKTAprime plus (product) Name) (manufactured by GE Healthcare Bioscience), 10 mM sodium phosphate-10 mM sodium citrate buffer solution (pH 7.5) (hereinafter referred to as buffer solution A) containing 0.7 M sodium sulfate was flowed at a flow rate of 1 mL / min. And the column was equilibrated. Next, 0.5 mL of a 1 mg / mL human plasma-derived immunoglobulin preparation (manufactured by Kakenken) dissolved in buffer A is added to the column, and after passing 10 mL of buffer A, 10 mM sodium phosphate- Concentration chromatography of sodium sulfate into 10 mM sodium citrate buffer (pH 7.5) was performed.

  Immunoglobulin eluted from the column was detected by measuring the absorbance at 280 nm. The eluate was fractionated by 1 mL, the absorbance at 280 nm of each fraction was measured with a U-2900 spectrophotometer (trade name) (manufactured by Hitachi, Ltd.), and the sum of them was measured at 280 nm of the immunoglobulin added to the column. The recovery rate was calculated by comparing with the absorbance. The result of chromatography is shown in FIG. The recovery rate of immunoglobulin in this chromatography was 100%.

  After the adsorption / desorption operation, 10 mL of 0.1 M sodium hydroxide was passed through to regenerate the column. The HiTrap column on which Compound 75 was immobilized could be restored to the initial state by the regeneration treatment, and its characteristics did not change even after repeated use.

Reference Example 3 Immobilization of Compound 75 on Vinyl Polymer Gel Compound 75 using TOYOPEARL AF-Amino-650M (trade name) (produced by Tosoh Corporation, amino group content: 89 μmol / mL (gel)) as an activation group-containing matrix Was fixed.

  Compound 75 is dissolved to a concentration of 22 mM in a mixed solvent (volume ratio of 1: 1) of 0.1M N-cyclohexyl-2-aminoethanesulfonic acid (hereinafter referred to as CHES) -NaOH buffer (pH 9.0) and acetonitrile. Then, 9.0 mL of this solution and 2.0 mL of TOYOPEARL AF-Amino-650M gel were mixed and shaken. After shaking for 30 minutes, 0.11 mL (1.1 mmol) of pyridine-borane was added, and the mixture was further shaken at 25 ° C. for 16 hours, and the gel after the fixation reaction was separated by suction filtration. The gel separated by filtration was washed 5 times with 5 mL of acetonitrile to obtain a gel in which compound 75 was immobilized. In addition, what combined this filtrate and the washing | cleaning liquid was made into the collection | recovery liquid.

  Separately, a solution containing pyridine-borane and Compound 75 was diluted with an eluent and analyzed by HPLC. As a result, Compound 75 was reduced to Compound 67. With this in mind, the reaction solution was analyzed by the HPLC method (detection wavelength 300 nm). As a result, it was found that 90 μmol of compound 67 was present in the recovered solution. That is, Compound 75 consumed by the immobilization reaction was 108 μmol, and the immobilization amount of Compound 75 was 54 μmol / mL (gel).

Reference Example 4 Immobilization of Compound 75 on Agarose Gel (Part 2)
Compound 75 was immobilized using EAH Sepharose 4B (trade name) (manufactured by GE Healthcare Biosciences, amino group content: 12 μmol / mL (gel)) as an activation group-containing matrix.

  5 mL of a solution of Compound 75 having the same composition as Reference Example 3 was added to 3.2 mL of EAH Sepharose 4B gel, and 0.29 mL (2.9 mmol) of pyridine-borane was further added, followed by shaking at 25 ° C. for 8 hours. . The gel after the immobilization reaction was separated by suction filtration, and the gel was washed 5 times with 5 mL of acetonitrile to obtain a gel immobilizing Compound 75.

  As a result of analyzing the combined liquid of the filtrate and the washing liquid by the same method as in Reference Example 3, the amount of Compound 75 immobilized was 27 μmol / mL (gel).

Reference Example 5 Immobilization of Compound 95 to Vinyl Polymer Gel Compound 95 using TOYOPEARL AF-Epoxy-650M (trade name) (manufactured by Tosoh Corporation, epoxy group content: 50 μmol / mL (gel)) as an activation group-containing matrix Was fixed.

  DMSO (5.5 mL), distilled water (4.18 mL), and 1 M NaOH aqueous solution (1.32 mL) were added to compound 95 (50.6 mg) to prepare a solution containing 15 mM of compound 95. 10 mL of this solution and 3 mL of TOYOPEARL AF-Epoxy-650M gel were mixed in a centrifuge tube and shaken at 40 ° C. for 70 hours. After the reaction, the centrifuge tube was centrifuged (100 × g) for 1 minute to separate the gel and the supernatant. After removing the supernatant, add 10 mL of DMSO to the gel, mix by stirring, centrifuge for 1 minute (100 × g), separate the gel and supernatant, wash the gel, and perform the above operations three times. It was. The reaction solution supernatant and the washing solution were mixed, and the volume up to 40 mL with DMSO was used as the recovered solution. Distilled water (30 mL) was added to the gel after washing with DMSO, mixed with stirring, centrifuged for 1 minute (100 × g), the gel and the supernatant were separated, and the supernatant was removed by suction. The above operation was performed 5 times to obtain a gel on which Compound 95 was immobilized.

  The amount of compound 95 immobilized measured by the absorbance method (detection wavelength: 348 nm) was 30 μmol / mL (gel).

Reference Example 6 Absorption and Desorption of Immunoglobulin Using Vinyl Polymer Gel Immobilized with Compound 95 1 mL of TOYOPEARL AF-Epoxy-650M gel immobilized with Compound 95 prepared by the method described in Reference Example 5 was added to a TRICORN column ( (Product Name) (GE Healthcare Biosciences Inc.) filled and attached to AKTAprime plus (Product Name) (GE Healthcare Biosciences Inc.) 0.5 mg human plasma-derived immunoglobulin preparation as in Reference Example 2 Were separated by chromatography on a sodium sulfate gradient. The chromatographic results are shown in FIG.

  After the adsorption / desorption operation, 10 mL of 0.1 M sodium hydroxide was passed through to regenerate the column. The epoxy toyopearl gel in which the compound 95 is immobilized can be restored to its initial state by this regeneration treatment, and its properties are not changed by repeated use.

Reference Example 7 Immobilization of Compound 95 on Agarose Gel Epoxy-activated Sepharose 6B (trade name) (manufactured by GE Healthcare Biosciences, epoxy group content: 19 to 40 μmol / mL (gel)) as an activation group-containing matrix The compound 95 was used for immobilization.

  Compound 95 is dissolved in a mixed solvent of 70 mM sodium hydroxide and N, N-dimethylacetamide (volume ratio 1: 1) to a concentration of 16 mM, and 6.0 mL of this solution and 3.1 mL of Epoxy-activated Sepharose 6B gel are mixed. And shaken at 40 ° C. for 2 hours. After the reaction, the gel was separated by suction filtration, and the gel was washed 5 times with 5 mL of the mixed solvent. These filtrate and washing solution were combined to obtain a recovered solution. The gel after washing with the mixed solvent was further washed twice with 10 mL of water to obtain a gel on which Compound 95 was immobilized.

  The amount of Compound 95 immobilized measured by the absorbance method (detection wavelength: 305 nm) was 32 μmol / mL (gel).

Reference Example 8 Immobilization of Compound 107 on Vinyl Polymer Gel Compound 107 using TOYOPEARL AF-Amino-650M (trade name) (produced by Tosoh Corporation, amino group content: 89 μmol / mL (gel)) as an activation group-containing matrix Was fixed.

  Compound 107 is dissolved in dehydrated DMF (manufactured by Wako Pure Chemical Industries, Ltd.) so that 21 mM, 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride is 41 mM, and NHS is 42 mM, and 15 mL of this solution and TOYOPEARL AF -2.1 mL of Amino-650M gel was mixed and shaken at 25 ° C for 4 days. After the reaction, the gel was separated by suction filtration, and washed with 5 mL of DMF 5 times. The filtrate and the washing solution were combined to obtain a recovered solution. The washed gel was suspended in 5 mL of acetic anhydride in DMF (acetic anhydride / DMF solution = 1/4 (volume ratio)) and shaken at 25 ° C. for 2 hours. Next, the gel was immobilized by suction filtration and washed with water to obtain a gel having the compound 107 immobilized thereon.

  The amount of immobilized Compound 107 measured by the absorbance method (detection wavelength 305 nm) was 11 μmol / mL (gel).

Reference Example 9 Immobilization of Compound 110 on Agarose Gel After passing 5 mL of DMSO through a HiTrap column, 2 mL of a solution dissolved in DMSO so that Compound 110 was 10 mM and triethylamine was 40 mM was passed. After standing for 1 hour, 3 mL of DMSO was passed through to remove unreacted compound 110. The liquid leaked from the column of the compound 110 solution used for the reaction and the liquid leaked from the DMSO column used for washing were combined to obtain a recovered liquid.

  The amount of compound 110 immobilized was calculated by subtracting the amount of compound 110 recovered from the column from the amount of compound 110 added to the column (20 μmol). A DMSO solution and a collected solution of Compound 110 prepared before immobilization were appropriately diluted according to “B. Acidic Condition Method” among the methods described in Non-Patent Document 9, and the absorbance at 306 nm was measured. From this method, the amount of the compound 110 immobilized on the HiTrap column was 2.3 μmol / mL (gel).

Reference Example 10 Immobilization of Compound 110 on Vinyl Polymer Gel Immobilization of Compound 110 using TOYOPEARL AF-Formyl-650M (trade name, manufactured by Tosoh Corporation, formyl group content 60 μmol / mL (gel)) as an activation group-containing matrix Was made.

  Compound 110 was dissolved in a mixed solvent of 0.1 M CHES-NaOH buffer (pH 9.0) and acetonitrile (volume ratio 1: 1) to a concentration of 36 mM, and 5.8 mL of this solution was added to TOYOPEARL AF-Formyl-650M. Mix and shake with 3.0 mL of gel, and after 30 minutes, add 0.17 mL (1.7 mmol) of pyridine-borane and shake for 16 hours at 25 ° C., and then filter the gel by suction filtration The gel separated by filtration was washed 5 times with 5 mL of acetonitrile. A combination of the filtrate and the washing solution was used as a recovered solution. The gel after washing was further washed twice with 10 mL of water to obtain a gel on which compound 110 was immobilized.

  The amount of Compound 107 immobilized measured by the HPLC method (detection wavelength: 254 nm) was 40 μmol / mL (gel).

Reference Example 11 Absorption and Desorption of Immunoglobulin Using Vinyl Polymer Gel Immobilized with Compound 110 1 mL of TOYOPEARL AF-Formyl-650M Gel Immobilized with Compound 110 prepared by the method described in Reference Example 10 was applied to a TRICORN column. Packed and attached to AKTAprime plus (trade name) (manufactured by GE Healthcare Biosciences), 0.5 mg of human plasma-derived immunoglobulin preparation was separated by chromatography with sodium sulfate concentration gradient in the same manner as in Reference Example 2. . The result of chromatography is shown in FIG.

  After the adsorption / desorption operation, 10 mL of 0.1M sodium hydroxide was passed through to regenerate the column. The TOYOPEARL AF-Formyl-650M gel on which Compound 110 was immobilized could be restored to its initial state by this regeneration treatment, and its properties did not change even after repeated use.

Claims (7)

General formula (1)

(Wherein R ¹ and R ² represent a hydrogen atom or a halogen atom, a hydroxy group, a carboxyl group, a methylthio group or a morpholinocarbonyl group optionally substituted with an alkyl group having 1 to 6 carbon atoms, and R ³ represents a mercapto group. A group, an aminoxy group (—ONH ₂ ), a phthalimidooxy group, or an alkoxythiocarbonylthio group having 1 to 6 carbon atoms, wherein Ar is one or more substituents selected from the following [1] to [16] in an optionally substituted phenyl group, a thiazole derivative pyridyl group or an furan-2-yl radical, n is represented by the representative.) an integer of 3 to 6.
[1] Halogen atom
[2] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, and a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a phenacyl group, a thienyl group, a furfuryl group, a pyridyl group or an acyl group, which may be substituted with an alkyl group having 1 to 6 carbon atoms
[3] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, or a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 12 carbon atoms which may be substituted with an alkoxycarbonyl group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a pyridyl group or an acyl group
[4] A C3-C8 cycloalkoxy group optionally substituted with a methyl group
[5] An alkenyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[6] An alkynyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[7] An acyloxy group having 1 to 6 carbon atoms which may be substituted with a halogen atom
[8] An alkylthio group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[9] An alkylsulfinyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[10] An alkylsulfonyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[11] An alkoxycarbonyl group having 1 to 12 carbon atoms which may be substituted with a halogen atom
[12] Carboxyl group
[13] Nitro group
[14] Methyl group, ethyl group optionally substituted with fluoro group, propyl group, isopropyl group, butyl group, isobutyl group, sec-butyl group, pentyl group, isoamyl group, neopentyl group, 2-methylbutyl group, hexyl Group, isohexyl group, 4-methylpentyl group, benzenesulfonyl group, p-toluenesulfonyl group, 4-chlorobenzenesulfonyl group, 4-nitrobenzenesulfonyl group, methylsulfonyl group, chloromethylsulfonyl group, trifluoromethylsulfonyl group, ethylsulfonyl Group, methoxycarbonyl group or amino group optionally substituted by ethoxycarbonyl group
[15] Hydroxy group
[16] Cyano group General formula (2)

(Wherein R ¹ represents a hydrogen atom or a halogen atom, a hydroxy group, a carboxyl group, a methylthio group or a morpholinocarbonyl group which may be substituted with an alkyl group having 1 to 6 carbon atoms, and n is an integer of 3 to 6) R ^3a represents a phthalimidooxy group or an alkoxythiocarbonylthio group having 1 to 6 carbon atoms, and Y represents a halogen atom.) And an α-haloketone body represented by the general formula (3)

(Wherein R ² represents a hydrogen atom or a halogen atom, a hydroxy group, a carboxyl group, a methylthio group or a morpholinocarbonyl group which may be substituted with a C 1-6 alkyl group, and Ar represents the following [1] To a phenyl group, a pyridyl group or a furan-2-yl group which may be substituted with one or more substituents selected from [16] .), And reacting with an acylthiourea derivative represented by And general formula (1a)

(Wherein R ¹ , R ² , R ^3a , Ar and n represent the same meaning as described above).
[1] Halogen atom
[2] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, and a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a phenacyl group, a thienyl group, a furfuryl group, a pyridyl group or an acyl group, which may be substituted with an alkyl group having 1 to 6 carbon atoms
[3] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, or a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 12 carbon atoms which may be substituted with an alkoxycarbonyl group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a pyridyl group or an acyl group
[4] A C3-C8 cycloalkoxy group optionally substituted with a methyl group
[5] An alkenyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[6] An alkynyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[7] An acyloxy group having 1 to 6 carbon atoms which may be substituted with a halogen atom
[8] An alkylthio group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[9] An alkylsulfinyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[10] An alkylsulfonyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[11] An alkoxycarbonyl group having 1 to 12 carbon atoms which may be substituted with a halogen atom
[12] Carboxyl group
[13] Nitro group
[14] Methyl group, ethyl group optionally substituted with fluoro group, propyl group, isopropyl group, butyl group, isobutyl group, sec-butyl group, pentyl group, isoamyl group, neopentyl group, 2-methylbutyl group, hexyl Group, isohexyl group, 4-methylpentyl group, benzenesulfonyl group, p-toluenesulfonyl group, 4-chlorobenzenesulfonyl group, 4-nitrobenzenesulfonyl group, methylsulfonyl group, chloromethylsulfonyl group, trifluoromethylsulfonyl group, ethylsulfonyl Group, methoxycarbonyl group or amino group optionally substituted by ethoxycarbonyl group
[15] Hydroxy group
[16] Cyano group General formula (1aa)

(In the formula, R ¹ and R ² represent a hydrogen atom or a halogen atom, a hydroxy group, a carboxyl group, a methylthio group or a morpholinocarbonyl group optionally substituted with an alkyl group having 1 to 6 carbon atoms ; (A phenyl group, a pyridyl group, or a furan-2-yl group which may be substituted with one or more substituents selected from [1] to [16] are represented, and n represents an integer of 3 to 6.) A thiazole derivative represented by the general formula (1ab) is reacted with hexacarbonylmolybten in the presence of water.

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above).
[1] Halogen atom
[2] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, and a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a phenacyl group, a thienyl group, a furfuryl group, a pyridyl group or an acyl group, which may be substituted with an alkyl group having 1 to 6 carbon atoms
[3] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, or a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 12 carbon atoms which may be substituted with an alkoxycarbonyl group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a pyridyl group or an acyl group
[4] A C3-C8 cycloalkoxy group optionally substituted with a methyl group
[5] An alkenyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[6] An alkynyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[7] An acyloxy group having 1 to 6 carbon atoms which may be substituted with a halogen atom
[8] An alkylthio group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[9] An alkylsulfinyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[10] An alkylsulfonyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[11] An alkoxycarbonyl group having 1 to 12 carbon atoms which may be substituted with a halogen atom
[12] Carboxyl group
[13] Nitro group
[14] Methyl group, ethyl group optionally substituted with fluoro group, propyl group, isopropyl group, butyl group, isobutyl group, sec-butyl group, pentyl group, isoamyl group, neopentyl group, 2-methylbutyl group, hexyl Group, isohexyl group, 4-methylpentyl group, benzenesulfonyl group, p-toluenesulfonyl group, 4-chlorobenzenesulfonyl group, 4-nitrobenzenesulfonyl group, methylsulfonyl group, chloromethylsulfonyl group, trifluoromethylsulfonyl group, ethylsulfonyl Group, methoxycarbonyl group or amino group optionally substituted by ethoxycarbonyl group
[15] Hydroxy group
[16] Cyano group General formula (1ac)

(In the formula, R ¹ and R ² represent a hydrogen atom or a halogen atom, a hydroxy group, a carboxyl group, a methylthio group or a morpholinocarbonyl group optionally substituted with an alkyl group having 1 to 6 carbon atoms ; [1] represents a phenyl group, a pyridyl group or a furan-2-yl group which may be substituted with one or more substituents selected from [16] , n represents an integer of 3 to 6, and R ⁴ represents an alkyl group having 1 to 6 carbon atoms) and is reacted under basic conditions (1ad)

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above).
[1] Halogen atom
[2] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, and a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a phenacyl group, a thienyl group, a furfuryl group, a pyridyl group or an acyl group, which may be substituted with an alkyl group having 1 to 6 carbon atoms
[3] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, or a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 12 carbon atoms which may be substituted with an alkoxycarbonyl group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a pyridyl group or an acyl group
[4] A C3-C8 cycloalkoxy group optionally substituted with a methyl group
[5] An alkenyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[6] An alkynyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[7] An acyloxy group having 1 to 6 carbon atoms which may be substituted with a halogen atom
[8] An alkylthio group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[9] An alkylsulfinyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[10] An alkylsulfonyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[11] An alkoxycarbonyl group having 1 to 12 carbon atoms which may be substituted with a halogen atom
[12] Carboxyl group
[13] Nitro group
[14] Methyl group, ethyl group optionally substituted with fluoro group, propyl group, isopropyl group, butyl group, isobutyl group, sec-butyl group, pentyl group, isoamyl group, neopentyl group, 2-methylbutyl group, hexyl Group, isohexyl group, 4-methylpentyl group, benzenesulfonyl group, p-toluenesulfonyl group, 4-chlorobenzenesulfonyl group, 4-nitrobenzenesulfonyl group, methylsulfonyl group, chloromethylsulfonyl group, trifluoromethylsulfonyl group, ethylsulfonyl Group, methoxycarbonyl group or amino group optionally substituted by ethoxycarbonyl group
[15] Hydroxy group
[16] Cyano group General formula (2)

(Wherein R ¹ represents a hydrogen atom or a halogen atom, a hydroxy group, a carboxyl group, a methylthio group or a morpholinocarbonyl group which may be substituted with a C 1-6 alkyl group, and R ^3a represents a phthalimidooxy group or An α-haloketone body represented by an alkoxythiocarbonylthio group having 1 to 6 carbon atoms, Y represents a halogen atom, and n represents an integer of 3 to 6, and a thiourea (3a)

In general formula (1b)

(Wherein R ¹ , R ^3a and n represent the same meaning as described above). General formula (1b)

(Wherein R ¹ represents a hydrogen atom or a halogen atom, a hydroxy group, a carboxyl group, a methylthio group or a morpholinocarbonyl group which may be substituted with a C 1-6 alkyl group, and R ^3a represents a phthalimidooxy group or A thiazole derivative represented by an alkoxythiocarbonylthio group having 1 to 6 carbon atoms, and n represents an integer of 3 to 6, and a general formula (4)

(In the formula, Ar represents a phenyl group, a pyridyl group or a furan-2-yl group which may be substituted with one or more substituents selected from the following [1] to [16] , and Z represents a halogen atom. An acid halide represented by the general formula (1af),

(Wherein R ¹ , R ^3a , Ar and n represent the same meaning as described above).
[1] Halogen atom
[2] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, and a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a phenacyl group, a thienyl group, a furfuryl group, a pyridyl group or an acyl group, which may be substituted with an alkyl group having 1 to 6 carbon atoms
[3] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, or a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 12 carbon atoms which may be substituted with an alkoxycarbonyl group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a pyridyl group or an acyl group
[4] A C3-C8 cycloalkoxy group optionally substituted with a methyl group
[5] An alkenyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[6] An alkynyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[7] An acyloxy group having 1 to 6 carbon atoms which may be substituted with a halogen atom
[8] An alkylthio group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[9] An alkylsulfinyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[10] An alkylsulfonyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[11] An alkoxycarbonyl group having 1 to 12 carbon atoms which may be substituted with a halogen atom
[12] Carboxyl group
[13] Nitro group
[14] Methyl group, ethyl group optionally substituted with fluoro group, propyl group, isopropyl group, butyl group, isobutyl group, sec-butyl group, pentyl group, isoamyl group, neopentyl group, 2-methylbutyl group, hexyl Group, isohexyl group, 4-methylpentyl group, benzenesulfonyl group, p-toluenesulfonyl group, 4-chlorobenzenesulfonyl group, 4-nitrobenzenesulfonyl group, methylsulfonyl group, chloromethylsulfonyl group, trifluoromethylsulfonyl group, ethylsulfonyl Group, methoxycarbonyl group or amino group optionally substituted by ethoxycarbonyl group
[15] Hydroxy group
[16] Cyano group General formula (1aa)

(In the formula, R ¹ and R ² represent a hydrogen atom or a halogen atom, a hydroxy group, a carboxyl group, a methylthio group or a morpholinocarbonyl group optionally substituted with an alkyl group having 1 to 6 carbon atoms ; A phenyl group, a pyridyl group or a furan-2-yl group which may be substituted with one or more substituents selected from [1] to [16] , and n represents an integer of 3 to 6. A thiazole derivative (1aa) represented by the general formula (1aa) is reacted under basic conditions

(Wherein R ¹ , R ² , Ar and n represent the same meaning as described above).
[1] Halogen atom
[2] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, and a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a phenacyl group, a thienyl group, a furfuryl group, a pyridyl group or an acyl group, which may be substituted with an alkyl group having 1 to 6 carbon atoms
[3] A halogen atom, a cycloalkyl group having 3 to 8 carbon atoms, a benzylthio group optionally substituted with a chloro group, a methylsulfinyl group, a methylsulfonyl group, or a carbon number 1 to 6 optionally substituted with a chloro group An alkoxy group having 1 to 12 carbon atoms which may be substituted with an alkoxycarbonyl group having 1 to 6 carbon atoms, a carboxyl group, an acetyl group, a pyridyl group or an acyl group
[4] A C3-C8 cycloalkoxy group optionally substituted with a methyl group
[5] An alkenyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[6] An alkynyloxy group having 3 to 6 carbon atoms which may be substituted with a halogen atom
[7] An acyloxy group having 1 to 6 carbon atoms which may be substituted with a halogen atom
[8] An alkylthio group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[9] An alkylsulfinyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[10] An alkylsulfonyl group having 1 to 6 carbon atoms which may be substituted with a halogen atom or a cycloalkyl group having 3 to 8 carbon atoms
[11] An alkoxycarbonyl group having 1 to 12 carbon atoms which may be substituted with a halogen atom
[12] Carboxyl group
[13] Nitro group
[14] Methyl group, ethyl group optionally substituted with fluoro group, propyl group, isopropyl group, butyl group, isobutyl group, sec-butyl group, pentyl group, isoamyl group, neopentyl group, 2-methylbutyl group, hexyl Group, isohexyl group, 4-methylpentyl group, benzenesulfonyl group, p-toluenesulfonyl group, 4-chlorobenzenesulfonyl group, 4-nitrobenzenesulfonyl group, methylsulfonyl group, chloromethylsulfonyl group, trifluoromethylsulfonyl group, ethylsulfonyl Group, methoxycarbonyl group or amino group optionally substituted by ethoxycarbonyl group
[15] Hydroxy group
[16] Cyano group

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