JP5734014B2 - Particle collector - Google Patents

Description

  The present invention relates to a particle collecting apparatus that collects microparticles such as bacteria from a fluid such as gas and liquid by using a filter or the like.

  In order to analyze fine particles in a fluid such as air or water, a mechanism for collecting the fine particles using a separation layer such as a filter is used (see Patent Document 1). Further, when analyzing fine particles, an analyzer constituted by a micro flow channel having a diameter of several hundred μm or less is often used. By using the microchannel structure, the dead volume of the analyzer is reduced, and a very small amount of sample and reagent can be reacted efficiently, enabling high-sensitivity analysis and reducing the consumption of samples and reagents. I can expect. Further, since the fine particles can be captured in a fine space, it is suitable for detailed observation of the particles.

Japanese Patent No. 2984161

Lab on a Chip, 2006, 6, 1125-1139.

  By the way, in the flow path for performing the analysis described above, in order to analyze the fine particles as the sample, as described above, it is important that the sample can be handled in a smaller volume. In other words, it is required to form finer. For example, a number of techniques for configuring an analyzer with microchannels having a diameter of several hundred μm or less have been developed (see Non-Patent Document 1). However, in these microchannel structures, the channel resistance increases due to the minute tube diameter and the complicated channel structure, and thus the flow rate that can pass through the channel structure is limited. For example, in many cases, it is not suitable for a process of continuously flowing an aqueous solution having a flow rate exceeding several ml / min.

  On the other hand, in order to efficiently collect particles using a filter, it is important to first increase the effective area of the filter and increase the amount of fluid that can be processed per unit time. Further, the path for transporting the fluid to be processed is required to have a large diameter in order to reduce the flow path resistance. In other words, when the filter is used in combination with a fine flow path, the effective area of the filter is reduced and the flow path resistance is increased, so that there is a problem that efficient particle collection cannot be performed.

  The present invention has been made to solve the above problems, and an object of the present invention is to enable more efficient particle collection with a filter combined with a fine flow path.

The particle collection device according to the present invention includes a flow path substrate having a flow path forming region on the inner surface, a bonding region formed on the inner surface surrounding the flow path forming region of the flow path substrate, and a flow channel in the bonding region. A separation layer that is bonded to the substrate and covers the flow path formation region; and a flow path mold including a convex region and a concave region formed in the flow path formation region of the flow channel substrate, the separation layer is a filter, The flow path substrate is formed in a state in which the convex region is separated from the separation layer, and in addition, can be deformed so that the convex region and the separation layer are in contact with each other, and the flow path formation region of the flow path substrate is deformed. Thus, the flow path is formed by the recessed area constituting the flow path mold by bringing the raised area and the separation layer into contact with each other.

  In the particle collecting apparatus, the surface of the separation layer may have hydrophobicity. Further, the separation layer may be capable of transmitting an aqueous solution.

  In the particle collecting apparatus, a support substrate is formed in which a concavo-convex portion corresponding to a flow path mold of the flow path substrate is formed, and the separation layer is sandwiched between the flow path substrate with the concavo-convex portion opposed to the flow path mold. You may make it prepare. In this case, you may make it provide the liquid which has the surface tension which is accommodated in the recessed part of an uneven | corrugated | grooved part and does not permeate | transmit a separation layer. Moreover, the flow path may be formed by the uneven part.

  The particle collecting apparatus may include a support substrate that is disposed in a state in which the separation layer is sandwiched between the flow path substrate. In addition, the support substrate has a gap between the region corresponding to the flow path formation region and the separation layer, and abuts the separation layer at a joint formed so as to surround the region of the flow path formation region. The support substrate in a region corresponding to the flow path forming region may be deformable so as to be in contact with the separation layer.

In addition, you may make it provide the input-output port which penetrates into a flow-path formation area from the outer surface of a flow-path board | substrate .

  As described above, according to the present invention, the flow path substrate is formed in a state where the convex region is separated from the separation layer, and can be deformed so that the convex region and the separation layer are in contact with each other. A filter combined with a simple flow path can provide an excellent effect that particle collection can be performed more efficiently.

FIG. 1 is a cross-sectional view showing the configuration of the particle collecting apparatus according to Embodiment 1 of the present invention. FIG. 2 is a plan view showing a partial configuration of the particle collecting apparatus according to Embodiment 1 of the present invention. FIG. 3 is a cross-sectional view showing a state in which the particle collecting apparatus according to Embodiment 1 of the present invention is deformed. FIG. 4 is a cross-sectional view showing the configuration of the particle collecting apparatus according to Embodiment 2 of the present invention. FIG. 5 is a cross-sectional view showing a state where the particle collecting apparatus in the second embodiment of the present invention is deformed. FIG. 6 is a cross-sectional view showing the configuration of the particle collecting apparatus according to Embodiment 3 of the present invention. FIG. 7 is a cross-sectional view showing the configuration of another particle collection device according to Embodiment 3 of the present invention. FIG. 8 is a cross-sectional view showing the configuration of the particle collecting apparatus according to Embodiment 4 of the present invention. FIG. 9 is a cross-sectional view showing the configuration of the particle collecting apparatus according to Embodiment 5 of the present invention. FIG. 10A is a cross-sectional view showing the configuration of the particle collection device according to Embodiment 6 of the present invention. FIG. 10B is a cross-sectional view showing a state where the particle collecting apparatus according to Embodiment 6 of the present invention is deformed. FIG. 11A is a cross-sectional view showing the configuration of the particle collection device according to Embodiment 7 of the present invention. FIG. 11B is a cross-sectional view showing a state where the particle collecting apparatus according to Embodiment 7 of the present invention is deformed. FIG. 12 is a plan view showing the configuration of the flow path structure of the particle collecting apparatus prepared for the experiment. FIG. 13 is a perspective view showing a partial configuration of a particle collecting apparatus prepared for an experiment. FIG. 14 is a perspective view showing a partial configuration of a particle collecting apparatus prepared for an experiment. FIG. 15 is a characteristic diagram showing the results of measuring the flow rate of air passing through the filter layer by connecting a suction pump to the outlet of the flow path to generate a negative pressure in each of the two states in the experiment.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

[Embodiment 1]
First, Embodiment 1 of the present invention will be described. FIG. 1 is a cross-sectional view showing the configuration of the particle collecting apparatus according to Embodiment 1 of the present invention. FIG. 2 is a plan view showing a partial configuration of the particle collecting apparatus according to Embodiment 1 of the present invention.

  This particle collecting apparatus includes a flow path substrate 101 having a flow path forming region 121 on the inner side surface, a bonding region 102 formed on the inner side surface surrounding the flow path forming region 121 of the flow path substrate 101, and a bonding region 102. The flow path is composed of a filter layer (separation layer) 103 that is bonded to the flow path substrate 101 and covers the flow path formation area 121, and a convex area 104 and a concave area 105 formed in the flow path formation area 121 of the flow path substrate 101. Road type.

  Further, the flow path substrate 101 is formed in a state in which the convex region 104 is separated from the filter layer 103, and can be deformed so that the convex region 104 and the filter layer 103 are in contact with each other. As shown in the plan view of FIG. 2, an input / output port 106 that penetrates from the outer surface of the channel substrate 101 to the channel forming region 121 is provided. In the example shown in FIG. 2, two input / output ports 106 are provided.

  As shown in the cross-sectional view of FIG. 3, the particle collection device according to the present embodiment deforms the flow channel formation region 121 of the flow channel substrate 101 to bring the convex region 104 and the filter layer 103 into contact with each other. The flow path is formed by the recessed area 105 constituting the flow path mold. For example, the flow path formation region 121 may be deformed by pressing the flow path substrate 101 and the filter layer 103 with a clamp or the like so that the convex region 104 and the filter layer 103 are brought into contact with each other. In addition, the inside where the convex region 104 and the concave region 105 are formed is depressurized via the input / output port 106 to reduce the internal pressure to atmospheric pressure or less, thereby forming a flow path due to the pressure difference between the outside and the inside. The convex region 104 and the filter layer 103 may be brought into contact with each other by deforming the region 121.

  First, as described with reference to FIG. 1, the particle collection device described above is configured so that the flow path substrate 101 and the filter inside the bonding region 102 are in a state where the convex region 104 is separated from the filter layer 103 (state A). A space 131 is formed between the layer 103. In addition, in the entire area of the space 131, the surface of the filter layer 103 on the flow path substrate 101 side is exposed.

  On the other hand, as described with reference to FIG. 3, in the state where the convex region 104 and the filter layer 103 are in contact with each other (state B), a flow path by the concave region 105 is formed. In this state, the surface of the filter layer 103 on the side of the flow path substrate 101 is exposed in the space (flow path space) of the recessed area 105 serving as the flow path.

  Here, when comparing the state A and the state B, first, the volume of the space between the flow path substrate 101 and the filter layer 103 is larger in the state A. Further, the area of the filter layer 103 exposed to the flow path substrate 101 side of the filter layer 103 is larger in the state A. In other words, the volume of the space between the flow path substrate 101 and the filter layer 103 is smaller in the state B. Further, the area of the filter layer 103 exposed to the flow path substrate 101 side of the filter layer 103 is smaller in the state B.

  Therefore, first, in the state A, a larger amount of fluid can flow between the flow path substrate 101 and the filter layer 103, and the filter layer 103 having a wider area is brought into contact with the flowed fluid. be able to. For this reason, the collection efficiency of the fine particles in the fluid flowing between the flow path substrate 101 and the filter layer 103 is higher in the state A.

  On the other hand, in the state B, the amount of fluid flowing between the flow path substrate 101 and the filter layer 103 can be limited to a small amount. For this reason, highly sensitive analysis is possible.

[Embodiment 2]
Next, a second embodiment of the present invention will be described. In the present embodiment, the filter layer 103 is prevented from being bent. In order to prevent the filter layer 103 from being bent in the state B described above, for example, a support substrate 401 may be used as shown in FIGS. The support substrate 401 is provided with a concavo-convex portion including a convex portion 402 and a concave portion 403 corresponding to the flow channel type of the flow channel substrate 101, and the filter layer 103 is connected to the flow channel substrate 101 with the concavo-convex portion facing the flow channel mold. Place it in a sandwiched state. Due to the presence of the support substrate 401, in the state B, as shown in FIG. 5, the convex region 104 of the flow path substrate 101 and the convex portion 402 of the support substrate 401 face each other and sandwich the filter layer 103. The bending of the layer 103 can be prevented.

  Further, the flow path may be formed by the convex portion 402 and the concave portion 403 (uneven portion) of the support substrate 401.

[Embodiment 3]
Next, a third embodiment of the present invention will be described. For example, when a reagent or the like is supplied to the flow path in the state B and analysis of the fine particles collected on the filter layer 103 in the flow path is performed, the supplied reagent may leak outside the filter layer 103. If the reagent leaks in this way, it will hinder accurate analysis. In such a case, for example, as shown in FIG. 6, a support substrate 601 that covers the outer surface of the filter layer 103 may be brought into contact with the outer surface of the filter layer 103. The support substrate 601 can suppress leakage of reagents and the like from the filter layer 103.

  Here, as illustrated in FIG. 7, in the state A, the support substrate 701 may be disposed in a state including the gap 702. The support substrate 701 includes a gap 702 between an area corresponding to the flow path forming area 121 and the filter layer 103, and a bonding formed so as to surround the area of the flow path forming area 121 in which the gap 702 is disposed. The portion 703 is brought into contact with the filter layer 103. In this state, filtration by the filter layer 103 can be performed. The support substrate 701 only needs to be deformable so that the region corresponding to the flow path forming region 121 is in contact with the filter layer 103 without the gap 702. This contacted state is the support substrate 601 shown in FIG.

[Embodiment 4]
Next, a fourth embodiment of the present invention will be described. In the present embodiment, for the purpose of suppressing leakage from the filter layer 103, as shown in FIG. 8, even when a liquid 801 having a surface tension that does not pass through the filter layer 103 is accommodated in the recess 403 of the support substrate 401. Good. If the liquid 801 is in contact with the outer surface of the filter layer 103, leakage from the filter layer 103 through the region can be suppressed.

[Embodiment 5]
Next, a fifth embodiment of the present invention will be described. In the present embodiment, in order to maintain the bonded state between the flow path substrate 101 and the filter layer 103 after the convex region 104 is brought into contact with the filter layer 103, as shown in FIG. Is provided with a vacuum chuck groove 901. The vacuum chuck groove 901 communicates with the outside through a connection hole 902 and can be connected to an exhaust mechanism (not shown). The vacuum chuck groove 901 may be formed at a location in contact with the filter layer 103 such as the convex region 104. After bringing the convex region 104 into contact with the filter layer 103, the vacuum chuck groove 901 is brought into a reduced pressure state, whereby the bonded state between the flow path substrate 101 and the filter layer 103 can be maintained more firmly.

[Embodiment 6]
Next, a sixth embodiment of the present invention will be described. 10A and 10B are configuration diagrams showing the configuration of the particle collecting device in the present embodiment. 10A and 10B schematically show cross sections.

  In the particle trapping apparatus, first, as in the first embodiment, the flow path substrate 101 having a flow path formation region (not shown) on the inner surface and the flow path substrate 101 bonded to each other at a bonding region (not shown). And a filter layer 103 covering the flow path forming region. In addition, two input / output ports 106 are provided. A support substrate 701 that covers the outer surface of the filter layer 103 is provided in contact with the outer surface of the filter layer 103. This configuration is the same as that of the third embodiment described above. In addition, although the flow path type | mold which consists of a convex part area | region and a recessed part area | region is formed in the flow path formation area of the flow path substrate 101, it is abbreviate | omitting in the figure.

  In addition to the above-described configuration, a pressure adjustment unit 1001 provided outside the flow path substrate 101 and a pressure adjustment unit 1002 provided on the support substrate 701 side are provided. The pressure adjusting unit 1001 includes a pressure chamber 1011 above the flow path forming region of the flow path substrate 101, and a flow port 1012 is connected to the pressure chamber 1011. Further, an input / output port 1013 communicating with the input / output port 106 is provided in a region other than the pressure chamber 1011. Similarly, the pressure adjusting unit 1002 includes a pressure chamber 1021 above the flow path forming region of the support substrate 701, and the flow port 1022 is connected to the pressure chamber 1021. An input / output port 1023 communicating with the input / output port 106 is provided in a region other than the pressure chamber 1021.

  As shown in FIG. 10B, the particle collecting apparatus in the present embodiment depressurizes the pressure chamber 1011 through the flow port 1012 and depressurizes the pressure chamber 1021 through the flow port 1022. Can be bent (deformed) to the pressure chamber 1011 side to form the space 131, and the support substrate 701 can be bent to the pressure chamber 1021 side to form the gap 702. In this manner, by forming the space 131 and the gap 702, more fluid can flow between the flow path substrate 101 and the filter layer 103, and a wider area with respect to the flowed fluid. The filter layer 103 can be contacted. For this reason, the collection efficiency of the fine particles in the fluid flowing between the flow path substrate 101 and the filter layer 103 can be improved as compared with the state shown in FIG. 10A.

[Embodiment 7]
Next, a seventh embodiment of the present invention will be described. FIG. 11A and FIG. 11B are configuration diagrams showing the configuration of the particle collecting device in the present embodiment. 11A and 11B schematically show a cross section.

  In the particle capturing apparatus, first, similarly to the above-described third embodiment, a flow path substrate 101 having a flow path forming region (not shown) on the inner side surface and a bonding region (not shown) bonded to the flow path substrate 101. And a filter layer 103 covering the flow path forming region. In addition, two input / output ports 106 are provided. A support substrate 701 that covers the outer surface of the filter layer 103 is provided in contact with the outer surface of the filter layer 103. In addition, although the flow path type | mold which consists of a convex part area | region and a recessed part area | region is formed in the flow path formation area of the flow path substrate 101, it is abbreviate | omitting in the figure.

  In addition to the above-described configuration, a pressure adjustment unit 1101 provided outside the flow path substrate 101 and a pressure adjustment unit 1102 provided on the support substrate 701 side are provided. The pressure adjustment unit 1101 includes a pressure chamber 1111 above the flow path formation region of the flow path substrate 101, and includes an input / output port 1113 communicating with the input / output port 106 in a region other than the pressure chamber 1111. Similarly, the pressure adjusting unit 1102 includes a pressure chamber 1121 above the flow path forming region of the support substrate 701, and an input / output port 1123 communicating with the input / output port 106 in a region other than the pressure chamber 1121.

  As shown in FIG. 11B, the particle collection device in the present embodiment, for example, depressurizes the pressure chamber 1121 and the pressure chamber 1111 via the input / output port 1123 with the input / output port 1113 closed. The flow path substrate 101 can be bent (deformed) toward the pressure chamber 1111 to form the space 131, and the support substrate 701 can be bent toward the pressure chamber 1121 to form the gap 702. In this manner, by forming the space 131 and the gap 702, more fluid can flow between the flow path substrate 101 and the filter layer 103, and a wider area with respect to the flowed fluid. The filter layer 103 can be contacted. For this reason, the collection efficiency of the fine particles in the fluid flowing between the flow path substrate 101 and the filter layer 103 can be improved as compared with the state shown in FIG. 11A.

[Experimental result]
Next, with respect to the actually produced particle collection device, the difference in flow path resistance between the state where the flow path formation region of the flow path substrate is in contact with the separation layer (filter layer) and the state where they are separated from each other And the result of having confirmed that the flow-path structure of a flow-path formation area functions as a flow path in the state which contacted is demonstrated.

  First, the flow channel structure shown in the plan view of FIG. 12 was formed. The circular portions at both ends of this flow path structure are the input / output ports 106. In addition, a flow path outlet through which the fluid that has passed through the filter layer passes is provided in the region 1201. The channel outlet provided in the region 1201 is a circle having a diameter of 2 mm in plan view. The flow path substrate having this flow path structure and the support substrate sandwiching the filter layer were made of polydimethylsiloxane (PDMS). The filter layer was composed of a polycarbonate membrane filter having a pore size of 0.4 μm.

  In the experiment, first, a suction pump is connected to the flow path outlet on the filter layer side, and the flow rate of air passing through the filter layer is measured while changing the pressure stepwise. The air flow rate was measured for both the state in which the filter layer and the flow path substrate were in contact and the state in which the filter layer and the flow path substrate were separated from each other, and the effect of pressure loss due to the flow path structure was confirmed.

  Next, in order to confirm that the filter layer and the flow path substrate are in contact with each other and to function as a micro flow path structure, an aqueous solution is injected into the flow path structure from the input / output section to confirm that no leakage occurs. did.

  Here, a state where the flow path substrate and the filter layer are separated is shown in a perspective view of FIG. As shown in FIG. 13, the filter layer 103 is separated from the flow path substrate 101 by separating the support substrate 601 from the flow path substrate 101 in the flow path formation region 121. Note that a flow path outlet 1301 is formed in the support substrate 601. In this way, the experiment was performed with a gap of about 5 mm between the flow path substrate 101 and the filter layer 103. The fluid can pass through the filter layer 103 without being affected by the flow path structure formed on the flow path substrate 101.

  Further, the state in which the flow path substrate and the filter layer are in contact with each other is shown in the perspective view of FIG. 14, and the flow path substrate 101 is brought into contact with the support substrate 601 as shown in FIG. It abuts on the flow path substrate 101. In this way, when the experiment is performed in a state where the flow path substrate 101 is in contact with the filter layer 103, the fluid passes through the filter layer 103 via the flow path structure, resulting in a pressure loss.

  FIG. 15 shows the result of measuring the flow rate of air passing through the filter layer by connecting a suction pump to the outlet of the flow path to generate negative pressure in each of the two states described above. The horizontal axis of the graph shown in FIG. 15 indicates the pressure at the flow path outlet, and the vertical axis indicates the flow rate of air passing through the filter layer. In FIG. 15, black squares indicate a state where the flow path substrate and the filter layer are separated, and “*” indicates a state where the flow path substrate and the filter layer are in contact with each other. In this experiment, for the sake of simplicity, an experiment was performed using a relatively simple flow path structure. In the state where the flow path substrate and the filter layer are separated (open) and in contact, the filter layer It can be confirmed that there is a difference in the flow rate of the air passing through the. In a finer channel structure used in an actual micro-analysis mechanism, it is easily guessed that the influence of pressure loss due to the channel structure becomes more prominent.

  In order to confirm that the filter layer and the flow path substrate are in contact with each other, the aqueous solution was injected into the flow path from one of the input / output ports. The support substrate, the filter layer, and the flow path substrate are in contact with each other due to their own weight and self-adhesiveness, but the aqueous solution flows along the flow path provided in the flow path substrate, and the aqueous solution bleeds into the convex wall surface portion. No leakage occurred and it was confirmed that the microchannel sufficiently functions.

  As described above, according to the present invention, the flow path substrate is formed in a state where the convex region is separated from the separation layer, and can be deformed so that the convex region and the separation layer are in contact with each other. The separation layer combined with the flow channel structure can efficiently collect particles while reducing pressure loss. Further, since the separation layer and the flow path structure for analysis are used in an integrated state, sample collection and analysis operation can be continuously performed in the same structure, and handling is easy. In addition, by configuring the flow path inner surface and the separation layer in a state of being blocked from the outside, an excellent effect is obtained that foreign substances are hardly mixed during the particle collection operation and the analysis operation. As described above, according to the present invention, a separation layer combined with a fine flow path can achieve both a high-flow sample processing and an analysis operation using a micro flow path, thereby enabling more efficient particle collection and analysis. Become.

  If such a structure of the present invention is used, for example, a microorganism contained in a trace amount in a gas and a liquid is separated and concentrated using a separation layer, and then the microorganism and the reagent are reacted using a portion of the microchannel. It is possible to obtain detailed information on the life and death, activity, and variety of microorganisms. There are many requests for cell analysis tools for detecting and analyzing cells in the industrial fields such as medicine, biotechnology, food, and hygiene equipment.

  The present invention is not limited to the embodiment described above, and many modifications and combinations can be implemented by those having ordinary knowledge in the art within the technical idea of the present invention. It is obvious. For example, the wall portion on the outer side (outermost circumference) of the flow path substrate may be constituted by a filter (separation layer), and a filter may be provided on a part of the wall portion. Further, a filter may be provided in the transport route of the flow path by the recessed region of the flow path substrate, and the transported fluid may be filtered by the filter when the flow path is configured.

  Next, the material of the flow path substrate is a material that ensures that the separation layer surface and the convex part of the flow path substrate material are in close contact with each other by contact and pressure bonding to the separation layer surface, and that no fluid leaks from the contact surface. Is desirable. Specifically, elastomers such as silicone rubber, synthetic rubber, and resin film are particularly suitable.

  The flow path substrate may be composed of a single material, or may be composed of a plurality of materials. For example, the deformed portion around the flow channel structure may be formed of a flexible material, and the flow channel structure portion that contacts the separation layer may be formed of a relatively hard material such as metal or resin. Further, even with a flow path substrate in which convex portions are formed of a relatively flexible resin material on a flat substrate such as glass, good adhesion between the separation layer surface and the flow path substrate can be realized.

  The dimensions of the flow path configured on the flow path substrate vary depending on the analysis object and the analysis content. For the purpose of reducing the channel resistance, it is better that the inner diameter of the channel is larger. However, in order to improve the analysis efficiency by using the microchannel structure, it is known that a channel structure with a diameter of several hundred μm or less is desirable. It has been. Moreover, although there is no designation | designated also regarding the cross-sectional shape of a flow path, shapes, such as a rectangle and a semicircle, are used well.

  The separation layer may be made permeable to an aqueous solution or the like. In this way, for example, a reagent or the like can be supplied from the separation layer side to the flow path formation region of the flow path substrate via the separation layer. Note that the particles captured by the separation layer can be washed out by supplying liquid to the flow channel side in a state where the separation layer and the flow channel substrate are in contact with each other. Further, in a contact state, a reagent may be supplied from the outside of the separation layer, and the supplied reagent may be reacted with particles that are thin in the separation layer.

  Further, the surface of the separation layer may have hydrophobicity. By doing in this way, it becomes possible to suppress the chemical solution supplied to the flow path formation region of the flow path substrate from leaking outside from the separation layer.

  The separation layer has a smooth contact surface with the flow path substrate material, and the contact surface between the flow path substrate and the convex portion of the flow path substrate material are securely adhered by contact and pressure bonding of the flow path substrate. A material that does not cause fluid leakage is desirable. The types of separation layers that can be used are track-etched membrane filters, membrane filters, microfiltration membranes, porous structures whose hydrophilicity has been adjusted by surface treatment, and porous structures in which materials that adsorb analytes are exposed. Is suitable. The separation layer can be made of a hydrophilic resin such as polycarbonate or nylon, or a hydrophobic resin such as fluororesin, and a separation layer with various surface treatments can be used to enhance functionality. Is possible.

  Further, the particle collecting apparatus of the present invention is not limited to the analysis of microorganisms, but can also be applied to analysis of bioaerosol, aerosol, chemical substance, dust, pollen, house dust, air pollution and the like. Moreover, not only the sample in air but the sample in a solution can also be used. In addition, the separation layer is not limited to a filter, and if it is replaced with an adsorbent or the like, it can be used for a wide range of analysis of chemical substances as well as solid particles.

  DESCRIPTION OF SYMBOLS 101 ... Channel board | substrate, 102 ... Joining area | region, 103 ... Filter layer (separation layer), 104 ... Projection area | region, 105 ... Concavity area | region, 106 ... Input-output port, 121 ... Channel formation area.

Claims (9)

A flow path substrate having a flow path forming region on the inner surface;
A bonding region formed on the inner surface surrounding the flow channel formation region of the flow channel substrate;
A separation layer that covers the flow path forming area by bonding to the flow path substrate in the bonding area;
A flow path mold comprising a convex region and a concave region formed in the flow channel forming region of the flow channel substrate,
The separation layer is a filter;
The flow path substrate is formed in a state in which the convex region is separated from the separation layer, and in addition, can be deformed into a state in which the convex region and the separation layer are in contact with each other,
The flow path is formed by the recessed area constituting the flow path mold by deforming the flow path forming area of the flow path substrate and bringing the convex area and the separation layer into contact with each other. To collect particles. The particle collection device according to claim 1,
The particle collecting apparatus, wherein the surface of the separation layer has hydrophobicity. The particle collection device according to claim 1,
The particle collecting apparatus, wherein the separation layer is permeable to an aqueous solution. In the particle collection device according to any one of claims 1 to 3,
A concavo-convex portion corresponding to the flow channel mold of the flow channel substrate is formed, and the support substrate is disposed so that the concavo-convex portion faces the flow channel mold and the separation layer is sandwiched between the flow channel substrate. A particle collecting apparatus characterized by that. The particle collecting apparatus according to claim 4,
A particle collecting apparatus comprising: a liquid having a surface tension which is accommodated in a concave portion of the concave and convex portion and does not pass through the separation layer. The particle collecting apparatus according to claim 4,
A particle collecting apparatus, wherein a flow path is formed by the uneven portion. In the particle collection device according to any one of claims 1 to 3,
A particle collecting apparatus comprising: a support substrate disposed in a state of sandwiching the separation layer with the flow path substrate. The particle collecting apparatus according to claim 7, wherein
The support substrate is
Abutting against the separation layer at a joint formed so as to surround the region of the flow path formation region with a gap between the region corresponding to the flow path formation region and the separation layer,
The particle collecting apparatus, wherein the gap is eliminated and the support substrate in a region corresponding to the flow path forming region can be deformed to abut against the separation layer. In the particle collection device according to any one of claims 1 to 8,
A particle collecting apparatus comprising an input / output port penetrating from the outer surface of the flow path substrate to the flow path forming region.