JP5713920B2 - グリコーゲン蓄積生物体におけるpha生産を最大にするためのプロセス - Google Patents
グリコーゲン蓄積生物体におけるpha生産を最大にするためのプロセス Download PDFInfo
- Publication number
- JP5713920B2 JP5713920B2 JP2011549694A JP2011549694A JP5713920B2 JP 5713920 B2 JP5713920 B2 JP 5713920B2 JP 2011549694 A JP2011549694 A JP 2011549694A JP 2011549694 A JP2011549694 A JP 2011549694A JP 5713920 B2 JP5713920 B2 JP 5713920B2
- Authority
- JP
- Japan
- Prior art keywords
- biomass
- gao
- vfa
- pha
- anaerobic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/343—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of grease, fat, oil
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
Claims (10)
- 混合培養バイオマスにおいて高ポリヒドロキシアルカノエート(PHA)生産能を有するグリコーゲン蓄積生物体(GAO)を選択することによって、廃水を処理し、且つポリヒドロキシアルカノエート(PHA)を生産する方法であって、
揮発性脂肪酸(VFA)及び混合培養バイオマスを含む廃水を供給すること;
相対的に高いレベルの細胞内貯蔵グリコーゲンを含むGAOを有するGAO優占バイオマスを生じる交互の嫌気性処理及び好気性又は無酸素処理に、該バイオマスを曝すこと;
該嫌気性処理中、該GAO優占バイオマス中のGAOが該VFA及び該細胞内貯蔵グリコーゲンを消費してPHAを生産するように、該好気性又は無酸素処理中より該嫌気性処理中のバイオマスへ、より多いVFAを供給すること;
供給された総浮遊固形物(TSS)の1gあたり少なくとも0.08gのVFAの比率で、嫌気性処理中のバイオマスに該VFAを供給すること
によって、
該GAO優占バイオマスを選択し、該バイオマスにおいて該GAOが増殖して、他の微生物より優占になるようにすること;
及び該GAO優占バイオマス内に該PHAを蓄積させることを含む、上記方法。 - GAO優占バイオマスを選択する前に、嫌気性条件下での前記廃水の処理、及び酸生成発酵を通して有機化合物を前記VFAに変換することをさらに含む、請求項1に記載の方法。
- 選択されたバイオマスを副流PHA生産ゾーンへ注ぎ、該PHA生産ゾーン内のバイオマスにVFAを給送することを含む、請求項1に記載の方法。
- 好気性又は無酸素処理相中、前記GAO優占バイオマスを収集し、該GAO優占バイオマスを第1のセパレータへ注ぎ、該GAO優占バイオマスを前記廃水から分離すること、及びその後、収集されたGAO優占バイオマスを、分離されたGAO優占バイオマスにVFAが給送される副流PHA生産ユニットへ注ぐことを含む、請求項1に記載の方法。
- 嫌気性条件下で処理される間に前記バイオマスに供給される前記VFAが、供給された総浮遊固形物(TSS)の1gあたり0.08〜0.50gのVFAである、請求項1に記載の方法。
- 前記バイオマス及び廃水を混合して混合液を形成し、該バイオマスを交互の嫌気性処理及び好気性又は無酸素処理に曝すことによって、該混合液中の総浮遊固形物に対して、好気性又は無酸素条件下で少なくとも18%の細胞内貯蔵グリコーゲンを含むGAOを有するGAO優占バイオマスが生じる、請求項1に記載の方法。
- 前記バイオマス及び廃水を混合して混合液を形成し、該バイオマスを交互の嫌気性処理及び好気性又は無酸素処理に曝すことによって、該混合液中のTSSに対して、好気性又は無酸素条件下で少なくとも30%の細胞内貯蔵グリコーゲンを含むGAOを有するGAO優占バイオマスが生じる、請求項1に記載の方法。
- 前記GAOが選択された後、引き続いて、VFAを前記バイオマスに給送して、該バイオマス中の該GAOが該VFAを消費し、PHAを生産するようにし、分離されたバイオマスに引き続いてVFAを給送するによって、前記廃水中のTSSに対して少なくとも45%のPHAが生じる、請求項1に記載の方法。
- 前記廃水をリアクターに注ぎ、該廃水を前記バイオマスで生物学的に処理することを含み、前記GAO優占バイオマス中のGAOが前記VFA及び前記細胞内貯蔵グリコーゲンを消費して、PHAを生産する、請求項1に記載の方法。
- 請求項9に記載の方法であって、前記廃水が相当なVFAを含むか、又は該方法が前記廃水中の有機物をVFAに変換することを含み、前記嫌気性処理相中にVFAが前記バイオマスに利用可能になっており、且つ該方法が、好気性又は無酸素処理相中のVFA負荷と比較して相対的に高いVFA負荷を前記嫌気性処理相中に供給することを伴う、上記方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US15194009P | 2009-02-12 | 2009-02-12 | |
US61/151,940 | 2009-02-12 | ||
PCT/IB2010/000281 WO2010092472A2 (en) | 2009-02-12 | 2010-02-12 | Process for maximizing pha production in glycogen accumulating organisms |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2012517237A JP2012517237A (ja) | 2012-08-02 |
JP5713920B2 true JP5713920B2 (ja) | 2015-05-07 |
Family
ID=42539532
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011549694A Expired - Fee Related JP5713920B2 (ja) | 2009-02-12 | 2010-02-12 | グリコーゲン蓄積生物体におけるpha生産を最大にするためのプロセス |
Country Status (8)
Country | Link |
---|---|
US (1) | US8187462B2 (ja) |
EP (1) | EP2396414A2 (ja) |
JP (1) | JP5713920B2 (ja) |
KR (1) | KR20110116045A (ja) |
CN (1) | CN102482694B (ja) |
AU (1) | AU2010212543B8 (ja) |
CA (1) | CA2752330C (ja) |
WO (1) | WO2010092472A2 (ja) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102770549B (zh) | 2009-12-10 | 2014-11-26 | 威立雅水务技术支持公司 | 使用在线监测进料速率控制和过程终止在生物质中积累聚羟基烷酸酯的方法 |
KR101220498B1 (ko) * | 2011-02-28 | 2013-01-10 | 한국과학기술원 | 유기산을 원료로 하여 미생물의 체내산물을 생산하는 방법 |
US9150445B2 (en) | 2011-08-09 | 2015-10-06 | Hsin-Ying Liu | Polyhydroxyalkanoate production during wastewater treatment |
US10807893B2 (en) | 2011-08-09 | 2020-10-20 | Hsinying Liu | Polyhydroxyalkanoate production during wastewater treatment |
ES2426671B2 (es) * | 2012-03-23 | 2014-05-14 | Universidade Da Coruña | Procedimiento de obtención de polihidroxialcanoatos a partir del agua residual de la industria cervecera. |
WO2013158042A1 (en) * | 2012-04-18 | 2013-10-24 | Nanyang Technological University | Method and apparatus for carbon dioxide capture and energy recovery enhancement |
SG11201406724WA (en) * | 2012-04-18 | 2014-11-27 | Univ Nanyang Tech | Method and apparatus for use in the treatment of water |
JP6016062B2 (ja) * | 2012-04-25 | 2016-10-26 | 国立大学法人広島大学 | ポリヒドロキシアルカノエートの生産装置及び生産方法 |
CN105189369B (zh) * | 2013-01-11 | 2017-05-03 | 威立雅水务技术支持公司 | 提高在混合培养生物质中的多羟基烷酸酯积聚的能力的生物废水处理方法 |
BR112015015650A2 (pt) | 2013-01-11 | 2017-07-11 | Veolia Water Solutions & Tech | método alimentado em batelada de alimentação sob demanda para produzir uma biomassa rica em pha a partir de culturas mistas abertas |
CN103332829B (zh) * | 2013-07-03 | 2015-01-14 | 同济大学 | 基于聚羟基烷酸酯代谢调控的污水增强生物除磷脱氮方法 |
EP3186200A2 (en) | 2014-08-04 | 2017-07-05 | Veolia Water Solutions & Technologies Support | Biofilm process for treating water with continuous or semi-continuous production of biomass with enhanced polyhydroxyalkanoate content |
US20170233271A1 (en) | 2014-08-07 | 2017-08-17 | Veolia Water Solutions & Technologies Support | Process for enhancing polyhydroxyalkanoate accumulation in activated sludge biomass |
MA40767A (fr) | 2014-10-01 | 2017-08-08 | Eggplant S R L | Procédés de production de composites à matrice biopolymère |
WO2016081902A1 (en) | 2014-11-20 | 2016-05-26 | Full Cycle Bioplastics Inc. | Producing polyhydroxyalkanoate copolymers from organic waste products |
CN106978346B (zh) * | 2017-04-21 | 2020-05-05 | 哈尔滨工业大学 | 一种提升pha合成菌产出段菌种排放量的方法 |
CN108486175A (zh) * | 2018-03-21 | 2018-09-04 | 河南师范大学 | 一种利用生物转换将污水中的碳源转化为pha并进行回收的方法 |
CN111596021B (zh) * | 2020-05-26 | 2022-02-11 | 同济大学 | 一种水体碳源质量评价方法、设备、装置及可读存储介质 |
CN114410565B (zh) * | 2022-01-27 | 2023-06-02 | 北京林业大学 | 一种利用污泥厌氧发酵液富集pha合成菌的方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6737263B2 (en) * | 2000-09-08 | 2004-05-18 | E. I. Du Pont De Nemours And Company | Polyhydroxyalkanoate levels as an indicator of bioreactor health |
-
2010
- 2010-02-12 CA CA2752330A patent/CA2752330C/en not_active Expired - Fee Related
- 2010-02-12 WO PCT/IB2010/000281 patent/WO2010092472A2/en active Application Filing
- 2010-02-12 JP JP2011549694A patent/JP5713920B2/ja not_active Expired - Fee Related
- 2010-02-12 AU AU2010212543A patent/AU2010212543B8/en not_active Ceased
- 2010-02-12 CN CN201080007749.9A patent/CN102482694B/zh not_active Expired - Fee Related
- 2010-02-12 EP EP10710423A patent/EP2396414A2/en not_active Withdrawn
- 2010-02-12 KR KR1020117020975A patent/KR20110116045A/ko active IP Right Grant
- 2010-02-12 US US12/704,732 patent/US8187462B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN102482694A (zh) | 2012-05-30 |
JP2012517237A (ja) | 2012-08-02 |
CA2752330A1 (en) | 2010-08-19 |
AU2010212543A8 (en) | 2014-10-02 |
AU2010212543B2 (en) | 2014-09-25 |
US20100200498A1 (en) | 2010-08-12 |
WO2010092472A3 (en) | 2010-12-02 |
AU2010212543B8 (en) | 2014-10-02 |
WO2010092472A2 (en) | 2010-08-19 |
CA2752330C (en) | 2017-01-03 |
EP2396414A2 (en) | 2011-12-21 |
US8187462B2 (en) | 2012-05-29 |
CN102482694B (zh) | 2014-07-09 |
KR20110116045A (ko) | 2011-10-24 |
AU2010212543A1 (en) | 2011-09-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5713920B2 (ja) | グリコーゲン蓄積生物体におけるpha生産を最大にするためのプロセス | |
Bengtsson et al. | A process for polyhydroxyalkanoate (PHA) production from municipal wastewater treatment with biological carbon and nitrogen removal demonstrated at pilot-scale | |
Guo et al. | Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor | |
EP2749650B1 (en) | Method for producing polyhydroxyalkanoates by microorganisms | |
Din et al. | Development of Bio-PORec® system for polyhydroxyalkanoates (PHA) production and its storage in mixed cultures of palm oil mill effluent (POME) | |
Bengtsson | The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates | |
CN112812280B (zh) | 一种生产聚羟基脂肪酸酯耦合反硝化脱氮的方法 | |
Tu et al. | Reducing the effect of non-volatile fatty acids (non-VFAs) on polyhydroxyalkanoates (PHA) production from fermented thermal-hydrolyzed sludge | |
EP3148942B1 (en) | Process for producing a microbial storage compound | |
Jayakrishnan et al. | Waste as feedstock for polyhydroxyalkanoate production from activated sludge: Implications of aerobic dynamic feeding and acidogenic fermentation | |
US9150445B2 (en) | Polyhydroxyalkanoate production during wastewater treatment | |
TWI661047B (zh) | 利用發酵廢棄污泥產生揮發性脂肪酸以增加污泥中聚羥基烷酸酯(polyhydroxyalkanoates,PHAs)含量之方法 | |
US11046604B2 (en) | Process for the treatment of wastewater containing organic material and ammonia | |
Salmiati et al. | Intracellular biopolymer productions using mixed microbial cultures from fermented POME | |
JP5869208B2 (ja) | 排水の処理方法及び排水の処理装置 | |
US11401537B2 (en) | Process for producing polyhydroxyalkanoate | |
Hamid et al. | The processing and treatment of other types of oil palm biomass | |
PT108093B (pt) | Processo para conversão de resíduos, efluentes e subprodutos orgânicos em materiais valorizáveis | |
Md Din et al. | Polyhydroxybutyrate (PHB) production from mixed cultures of sewage sludge and palm oil mill effluent (POME): The influence of C/N ratio and slow accumulation factor | |
Fei | Impact of additional carbon on poly-hydroxybutyrates (PHB) accumulation and nutrient removal in a sustainable anaerobic/anoxic/oxic (A2O) membrane bioreactor | |
Bratby et al. | The dirty secret of primary sludge prefermenters | |
Sardo | Selection Of Phb-producing Bacteria By Using Waste Streams | |
Vanotti et al. | Streamlined ammonia removal from wastewater using biological deammonification process | |
UJANG_______ | BORANG PENGESAHAN STATUS TESIS♦ JUDUL: POLYHYDROXYBUTYRATE (PHB) PRODUCTION FROM GREY WATER USING AEROBIC-INTERMITTENT FEEDING | |
JP2005287317A (ja) | 水素の製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20130107 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140613 |
|
A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140826 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20150303 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20150310 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5713920 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
LAPS | Cancellation because of no payment of annual fees |