JP5667456B2 - フリーラジカルの捕捉による溶解動的核分極(dnp)を使用したnmr分光測定またはmri測定の方法 - Google Patents
フリーラジカルの捕捉による溶解動的核分極(dnp)を使用したnmr分光測定またはmri測定の方法 Download PDFInfo
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/28—Details of apparatus provided for in groups G01R33/44 - G01R33/64
- G01R33/282—Means specially adapted for hyperpolarisation or for hyperpolarised contrast agents, e.g. for the generation of hyperpolarised gases using optical pumping cells, for storing hyperpolarised contrast agents or for the determination of the polarisation of a hyperpolarised contrast agent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
- G01R33/48—NMR imaging systems
- G01R33/54—Signal processing systems, e.g. using pulse sequences ; Generation or control of pulse sequences; Operator console
- G01R33/56—Image enhancement or correction, e.g. subtraction or averaging techniques, e.g. improvement of signal-to-noise ratio and resolution
- G01R33/5601—Image enhancement or correction, e.g. subtraction or averaging techniques, e.g. improvement of signal-to-noise ratio and resolution involving use of a contrast agent for contrast manipulation, e.g. a paramagnetic, super-paramagnetic, ferromagnetic or hyperpolarised contrast agent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
- G01R33/48—NMR imaging systems
- G01R33/54—Signal processing systems, e.g. using pulse sequences ; Generation or control of pulse sequences; Operator console
- G01R33/56—Image enhancement or correction, e.g. subtraction or averaging techniques, e.g. improvement of signal-to-noise ratio and resolution
- G01R33/5605—Image enhancement or correction, e.g. subtraction or averaging techniques, e.g. improvement of signal-to-noise ratio and resolution by transferring coherence or polarization from a spin species to another, e.g. creating magnetization transfer contrast [MTC], polarization transfer using nuclear Overhauser enhancement [NOE]
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- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Magnetic Resonance Imaging Apparatus (AREA)
Description
1.1)適当な、特にガラス形成溶媒に含まれる調査中の溶質に加えて、常磁性体、特にラジカルを含む第1種の凍結ビーズを調製するステップと、
2.1)分極マグネットへその凍結ビーズを挿入するステップと、
3.1)低温の磁場中で、試料中の核の高い分極状態を作り出すステップと、
4.1)試料を室温まで加熱するステップと、
5.1)分極した試料を分極マグネットからMRマグネットへ移すステップと、
6.1)MR測定を行うステップと
を含む、溶解動的核分極による過分極を使用した、磁気共鳴(=MR)測定用の試料を調製する方法に関する。
1.2)溶媒に溶解した還元剤を含む第2種の凍結ビーズを調製するステップ、
2.2)第2種の凍結ビーズを、第1種の凍結ビーズと共に分極マグネットへ挿入するステップ、
を含む。
Claims (15)
- 1.1)溶媒中に、調査中の溶質に加えて、常磁性ラジカルを含む第1種の凍結ビーズを調製するステップと、
2.1)分極マグネットへ前記凍結ビーズを挿入するステップと、
3.1)低温の磁場中で、試料中の核の高い分極状態を作り出すステップと、
4.1)前記試料を室温まで加熱するステップと、
5.1)前記分極化した試料を前記分極マグネットからMRマグネットへ移すステップと、
6.1)MR測定を行うステップとを含む、溶解動的核分極による過分極を使用した、磁気共鳴(=MR)測定用の試料を調製する方法であって、
追加のステップとして、
1.2)他の溶媒に溶解した、フリーラジカルを還元するための還元剤を含む第2種の凍結ビーズを調製するステップと、
2.2)前記第2種の凍結ビーズを、前記第1種の凍結ビーズと共に前記分極マグネットへ挿入するステップとを含むことを特徴とする方法。 - 前記溶媒は、ガラス形成溶媒であることを特徴とする請求項1記載の方法。
- 前記第2種のビーズ中の前記還元剤の濃度を示す第1の濃度が、前記第1種のビーズ中の前記常磁性ラジカルの濃度を示す第2の濃度よりも高いことを特徴とする請求項1又は2記載の方法。
- 前記第1の濃度は、前記第2の濃度の2倍から100倍高いことを特徴とする請求項3記載の方法。
- 前記還元剤が、アスコルビン酸ナトリウムであることを特徴とする請求項1乃至4のいずれか1項に記載の方法。
- 前記還元剤が、水に溶解していることを特徴とする請求項1乃至5のいずれか1項に記載の方法。
- 前記還元剤が溶解している水は重水であることを特徴とする請求項6記載の方法。
- 前記第1種のビーズ中の前記常磁性ラジカルが、ニトロキシルラジカルを含むことを特徴とする請求項1乃至7のいずれか1項に記載の方法。
- 前記第1種のビーズ中の前記常磁性ラジカルが、4−ヒドロキシ−2,2,6,6−テトラメチルピペリジン−1−オキシル(=TEMPOL)を含むことを特徴とする請求項1乃至8のいずれか1項に記載の方法。
- 前記第1種の凍結ビーズの前記溶媒が、グリセロールおよび水の混合物であることを特徴とする請求項1乃至9のいずれか1項に記載の方法。
- 前記混合物中の水は重水であることを特徴とする請求項10記載の方法。
- 前記第1種および前記第2種のビーズが、前記分極マグネットへ同質量挿入されることを特徴とする請求項1乃至11のいずれか1項に記載の方法。
- ステップ3.1)において低温の磁場中でマイクロ波を照射することによって、前記試料中の核の分極を高めた状態を作り出すことを特徴とする請求項1乃至12のいずれか1項に記載の方法。
- ステップ6.1)における前記MR測定が、核磁気共鳴(=NMR)分光計または磁気共鳴映像(MRI)装置で行われることを特徴とする請求項1乃至9のいずれか1項に記載の方法。
- 前記磁気共鳴映像(MRI)装置は断層撮影装置であることを特徴とする請求項14記載の方法。
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EP10150983.4 | 2010-01-18 | ||
EP10150983.4A EP2348327B1 (en) | 2010-01-18 | 2010-01-18 | Method for NMR measurements using dissolution dynamic nuclear polarization (DNP) with elimination of free radicals |
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CN110082381B (zh) * | 2019-05-08 | 2021-12-03 | 中国科学院武汉物理与数学研究所 | 一种利用dnp-mri测量油水分布方法 |
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JP3682044B2 (ja) * | 2000-11-03 | 2005-08-10 | アメルシャム ヘルス アクスイェ セルスカプ | Nmr分析用の過分極固体物質を溶解するデバイス及びその方法 |
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US7906962B2 (en) | 2007-08-24 | 2011-03-15 | The Regents Of The University Of California | Dynamic nuclear polarization enhanced nuclear magnetic resonance of water under ambient conditions |
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EP2348327A1 (en) | 2011-07-27 |
US8564288B2 (en) | 2013-10-22 |
JP2011145297A (ja) | 2011-07-28 |
US20110175611A1 (en) | 2011-07-21 |
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