JP5608435B2 - Anti-ice nuclear activator and method for producing the same - Google Patents

Description

  The present invention relates to an anti-ice nucleus activator containing an extract from sake and a method for producing the same.

  In general, water coagulates at a temperature below the melting point (0 ° C.), but pure water containing no foreign matter does not coagulate to −39 ° C. Thus, the phenomenon in which water does not solidify even when cooled to a temperature below the melting point is called a supercooling phenomenon. This supercooling phenomenon is necessary and sufficient activation to generate ice nuclei, which are the seeds of ice necessary for water to become ice, as the temperature of water decreases and the kinetic energy of water molecules decreases. This is caused by the lack of energy.

  Such a substance that promotes supercooling is called a supercooling promotion (anti-ice nucleus activity) substance, and as a substance exhibiting anti-ice nucleus activity, eugenol (see Non-Patent Document 1) that is a component of spices Low molecular weight compounds such as hinokitiol-iron (see Non-Patent Document 2), a component of Taiwan cypress, proteins derived from Acinetobacter calcoaceticus (see Non-Patent Document 3), polysaccharides derived from Bacillus thuringiensis (see Non-Patent Document 4), etc. It has also been reported that polymer compounds exhibit anti-ice nucleus activity.

  Further, as an anti-ice nucleus active substance exhibiting higher ice nucleus activity, flavonoid glycosides isolated from softwood wig parenchyma cells have been clarified (see Patent Document 1). Of the saccharides, it has been reported that a compound in which the hydroxyl group at the 7-position is glucosylated exhibits high activity (see Non-Patent Document 5).

  However, these anti-ice nucleoactive substances can reduce the increase in the supercooling point due to ice nucleation active bacteria contained in water, but the supercooling point due to foreign matter showing ice nucleation activity such as silver iodide. In addition, these compounds have been difficult to use in the food field due to safety and productivity problems.

  Therefore, in order to make use in the food field and to make effective use of food waste, the present applicant has found that the extract extracted from the koji produced in the production process of ginger shows anti-ice nuclear activity, A patent application has already been filed for an anti-freezing active agent containing such an extract of koji (see Patent Document 2). Thus, by obtaining an anti-ice nucleus active substance from straw, the obtained anti-active substance can be applied not only to the food field but also to effectively use food waste.

WO2008 / 007684 JP 2010-121052 A

H. Kawahara et al, J. Antibact. Antifung. Agents, 24, 95-100 2006 H. Kawahara et al, Biosci. Biotech. Biochem., 64, 2651-2656, 2000 H. Kawahara et al, Biocontrol Sci, 1, 11-17, 1996 Y. Yamashita et al, Biosci, Biotech. Biochem., 66, 948-954, 2002 J. Kasuga et al., Cryobiology, 60, 240-243 2010

  However, the amount of anti-ice nuclear active substance derived from straw depends on the amount of straw produced, that is, the amount of ginger produced, and the supply of anti-ice nuclear active substance may become unstable. Therefore, it has been desired to obtain an anti-ice nucleus active substance from foods other than koji.

  On the other hand, sake has been marketed in the past, but the remaining sake and the expiration date of the sake after the market are collected by the company, the amount of which is larger than the amount of sake. If the sake collected in this way can be used effectively, waste is reduced in both the cost and the environment of the company.

  In view of the above-mentioned problems, the present invention exhibits a wide range of anti-ice nucleation activity for ice nuclei active substances, can be applied to the food field, and can be obtained from other than anti-ice nuclei activity. It is an object to provide an agent and a method for producing the same.

  As a result of intensive studies to solve such problems, the present inventor has found that the extracted component extracted from sake has excellent anti-ice nuclear activity, and has completed the present invention. That is, the present invention is an anti-ice nucleus activator characterized by containing an extract component from sake. As a result, the anti-ice nucleus activator of the present invention exhibits a wide anti-ice nucleus activity action not only on ice nucleus active bacteria but also on ice nucleus active substances such as silver iodide, and is an extracted component from Japanese sake. Can be obtained from sake other than sake.

In addition, the anti-ice nucleus activator of the present invention adsorbs a component of sake to a styrene-divinylbenzene synthetic adsorbent and contains an adsorbate adsorbed on the synthetic adsorbent as an extract component from sake . Thereby, the anti-ice nucleus activity of an extraction component can be improved more.

The anti-ice nucleus activator of the present invention contains the adsorbate extracted with ethyl acetate, and the extract extracted with the ethyl acetate is contained as an extraction component from sake . Thereby, the anti-ice nucleus activity of an extraction component can be improved more.

In addition, the anti-ice nucleus activator of the present invention adsorbs a component of sake to a styrene-divinylbenzene synthetic adsorbent, and the adsorbate adsorbed on the synthetic adsorbent is fractionated to a molecular weight of 3000 or less by an ultrafiltration membrane. And a fraction having a molecular weight of 3000 or less is contained as an extract component from sake . Thereby, the anti-ice nucleus activity of an extraction component can be improved more.

Further, the anti-ice nucleus activator of the present invention fractionates the extract extracted with ethyl acetate into a fraction having a molecular weight of 3000 or less by an ultrafiltration membrane, and contains the fraction having a molecular weight of 3000 or less as an extract component from sake. It is preferable to do. Thereby, the anti-ice nucleus activity of an extraction component can be improved more.

The present invention, styrene-containing component sake - an adsorption step of adsorbing the divinylbenzene-based synthetic adsorbent, a adsorbate adsorbed on the synthetic adsorbent, and a extraction step of extracting with ethyl acetate, the ethyl acetate It is the manufacturing method of the anti-ice nucleus activator characterized by including the extract extracted by (5) as an extraction component from sake. As a result, it exhibits a wide range of anti-ice nucleation activity not only for ice nucleation active bacteria but also ice nucleation active substances such as silver iodide, and since it is an extracted component from sake, it can be applied to the food field. It is possible to obtain an anti-ice nucleus active agent that can be obtained from other sake.
The present invention also includes an adsorption step of adsorbing sake-containing components on a styrene-divinylbenzene synthetic adsorbent and fractionating the adsorbate adsorbed on the synthetic adsorbent into a fraction having a molecular weight of 3000 or less by an ultrafiltration membrane. A fractionation step, wherein the fraction having a molecular weight of 3000 or less is contained as an extraction component from sake.
In addition, the present invention further includes a fractionation step of fractionating the extract extracted with ethyl acetate into a fraction having a molecular weight of 3000 or less by an ultrafiltration membrane, and the fraction having a molecular weight of 3000 or less is used as an extraction component from sake. It is preferable to contain.

  ADVANTAGE OF THE INVENTION According to this invention, the anti-ice nucleus active agent which shows the anti-ice nucleus activity action widely about an ice nucleus active substance, can be applied to the food field | area, and can be obtained from things other than a candy, and its manufacture A method can be provided.

Chart showing the ultraviolet absorption of each fraction when gel filtration chromatography is performed on the adsorbed fraction 2A is a fraction showing peak 1 in FIG. 1, FIG. 2B is a fraction showing peak 2 in FIG. 1, and FIG. 2C is an ultraviolet absorption of a fraction showing peak 3 in FIG. Chart showing spectrum

  The anti-ice nucleus activator of the present invention is an anti-ice nucleus activator containing an extract component from sake. In the present invention, "containing an extract component from sake" means that the anti-ice nucleus activator of the present invention may contain only an extract component from sake, and in addition to an extract component from sake, It means that other components may be contained.

  Sake is also known as refined sake, which is made by fermenting rice, rice bran and water as raw materials, and by adding auxiliary materials such as brewed alcohol, sugar, acidulant, umami seasoning and sake lees to these ingredients. Say something. There are no particular limitations on the variety or production area of rice, which is the raw material for sake.

  Such an anti-ice nucleus activator of the present invention has a broad anti-ice nucleus activity action not only on ice nucleus active bacteria but also on ice nucleus active substances such as silver iodide, and since it is an extractive component from Japanese sake, It can be applied and can be obtained from sake other than sake. In addition, since the collected sake can be used, it can be produced in larger quantities, and the food waste can be used more effectively.

  The anti-ice nucleus activator of the present invention preferably contains a sake-containing component adsorbed on a synthetic adsorbent, and contains an adsorbate adsorbed on the synthetic adsorbent as an extraction component from sake. Thereby, since the component which has anti-ice nucleus activity is contained more in an extraction component, anti-ice nucleus activity can be exhibited more. The synthetic adsorbent is preferably a styrene-divinylbenzene synthetic adsorbent. Examples of the styrene-divinylbenzene synthetic adsorbent include Diaion HP21 (Mitsubishi Chemical Co., Ltd.), which is a styrene-divinylbenzene copolymer, and Sepabead SP207 (Mitsubishi, Ltd.) obtained by adding bromine to a styrene-divinylbenzene copolymer. Chemical Co., Ltd.).

  Moreover, it is preferable that the anti-ice nucleus activator of this invention extracts the said adsorbate with ethyl acetate, and contains the extract extracted with this ethyl acetate as an extraction component from sake. Thereby, since the extract component contains more components having anti-ice nucleus activity, the anti-ice nucleus activity can be further improved.

  Moreover, it is preferable that the anti-ice nucleus activator of this invention fractionates the said adsorbate into a fraction with a molecular weight of 3000 or less by an ultrafiltration membrane, and contains this fraction with a molecular weight of 3000 or less as an extraction component from sake. Thereby, since the extract component contains more components having anti-ice nucleus activity, the anti-ice nucleus activity can be further improved.

  Moreover, it is preferable that the anti-ice nucleus activator of this invention fractionates the said extract into a molecular weight 3000 or less fraction with an ultrafiltration membrane, and contains this molecular weight 3000 or less fraction as an extraction component from sake. Thereby, since the extract component contains more components having anti-ice nucleus activity, the anti-ice nucleus activity can be further improved.

  Thus, when fractionating into fractions having a molecular weight of 3000 or less, the adsorbate adsorbed on the synthetic adsorbent can be fractionated, or the extract extracted with ethyl acetate can be fractionated. However, considering the viewpoint of further improving the anti-ice nucleus activity, it is more preferable that the anti-ice nucleus activator of the present invention fractionates an extract extracted with ethyl acetate into a fraction having a molecular weight of 3000 or less. .

  In order to obtain the anti-ice nucleus activator as described above, the method for producing an anti-ice nucleus activator of the present invention includes an adsorption step of adsorbing sake-containing components to the synthetic adsorbent, and is adsorbed to the synthetic adsorbent. The adsorbent is contained as an extract component from sake. Thereby, since the extraction component in which the component which has anti-ice nucleus activity is contained more can be obtained, the anti-ice nucleus activity of an extraction component can be improved more. Specifically, the components of sake can be adsorbed on the synthetic adsorbent in the adsorption step, and the adsorbate adsorbed on the synthetic adsorbent can be dissolved in ethanol, and then concentrated and dried with an evaporator to obtain the extracted component. it can.

  The method for producing an anti-ice nucleus activator of the present invention includes an extraction step of extracting the adsorbate adsorbed on the synthetic adsorbent in the adsorption step with ethyl acetate, and the extract extracted with ethyl acetate is extracted from sake. It is preferable to contain as an extraction component. Thereby, since the extraction component in which the component which has anti-ice nucleus activity is contained more can be obtained, the anti-ice nucleus activity of an extraction component can be improved more. Specifically, after the extraction components concentrated and dried by an evaporator in the adsorption step are dissolved in water, the conditions are set to acidic conditions. After further adding ethyl acetate, the ethyl acetate layer is separated with a separatory funnel, etc. The extract component can be obtained by concentrating and drying the ethyl layer.

  The method for producing an anti-ice nuclear activator of the present invention comprises a fractionation step of fractionating the adsorbate adsorbed on the synthetic adsorbent in the adsorption step into a fraction having a molecular weight of 3000 or less using an ultrafiltration membrane. It is preferable to contain a fraction having a molecular weight of 3000 or less as an extract component from sake. Thereby, since the extraction component in which the component which has anti-ice nucleus activity is contained more can be obtained, the anti-ice nucleus activity of an extraction component can be improved more.

  The method for producing an anti-ice nucleus activator of the present invention includes a fractionation step of fractionating the extract extracted with ethyl acetate in the extraction step into a fraction having a molecular weight of 3000 or less using an ultrafiltration membrane. It is preferable to contain a fraction having a molecular weight of 3000 or less as an extract component from sake. Thereby, since the extraction component in which the component which has anti-ice nucleus activity is contained more can be obtained, the anti-ice nucleus activity of an extraction component can be improved more. As described above, considering the viewpoint of further improving the anti-ice nucleus activity, the method for producing the anti-ice nucleus activator of the present invention is to separate the extract extracted with ethyl acetate into a fraction having a molecular weight of 3000 or less. More preferably.

  The extraction component obtained by the above production method may be used as a solid as it is, or may be used after further purification. In addition, various means and methods can be used to purify the extracted component, such as various chromatographic methods such as ion exchange chromatography and gel filtration chromatography, ultrafiltration using a cellulose membrane or a synthetic membrane, Separation methods such as reverse osmosis, adsorption, and organic solvent fractionation can be used.

  If necessary, if the extracted component becomes a precipitate in the solvent, centrifugation may be performed. As described later, most of the anti-ice nucleus activity in the extractive component of the present invention is present in a fraction having a molecular weight of 3000 or less when fractionated using an ultrafiltration membrane. It is preferable from the viewpoint of efficiency improvement.

  The anti-ice nucleus activator of the present invention contains an extracted component from sake as an active ingredient. In this case, the extracted component may be used after being processed into a liquid or solid (powder, granule, etc.). In addition, when the extraction component is dissolved in a solvent, it may be used by appropriately concentrating or diluting, and examples of the solvent include water. In any case, the form of the extraction component can be appropriately designed according to its use.

  Furthermore, the dosage form for formulating the anti-ice nucleus active agent of the present invention is not particularly limited, and may be a solution, suspension, emulsion, tablet, capsule, granule, powder, cream, ointment, etc. Any type.

  The anti-ice nucleus activator of the present invention is a food field such as a frozen food quality preservation agent, a frost damage control agent, an environmental field such as an unfrozen beverage, a frost adhesion inhibitor, and a medical field such as a cell preservation solution and an organ preservation solution. Can be widely applied to etc.

  Examples of the present invention will be described below.

(Example 1) Acquisition of extracted components by adsorption onto a synthetic adsorbent Japanese sake (manufactured by Kikumasou Shuzo, raw material name: rice, rice bran, milled rice ratio: 65%) 3.6L, an evaporator set at 60 ° C Concentrate to 400 mL.

  135 g of Diaion HP21 (manufactured by Mitsubishi Chemical Corporation, hereinafter referred to as HP21 resin), which is a styrene-divinylbenzene synthetic adsorbent, was added to the concentrated liquid obtained, and the mixture was stirred for 18 hours at 4 ° C. and 120 rpm using a stirrer. Thereafter, suction filtration was performed using filter paper. 50% ethanol was added to the HP21 resin remaining on the filter paper, and the mixture was stirred overnight at 4 ° C. and 120 rpm using a stirrer to dissolve the adsorbate adsorbed on the HP21 resin in ethanol.

  Thereafter, the mixed solution of HP21 resin and ethanol was suction filtered again using filter paper, and the obtained filtrate (adsorbed solution) was concentrated and dried with an evaporator set at 60 ° C., and adsorbed from sake to the HP21 resin. As an extraction component of Example 1, 4.81 g of an adsorbed fraction was obtained.

(Example 2) Acquisition of extracted components by extraction with ethyl acetate The extracted components of Example 1 were dissolved in ultrapure water to adjust the concentration to 10 mg / mL, and 12N HCl was added thereto to add acidic pH 3.0. The amount was adjusted to the conditions, twice as much ethyl acetate as the aqueous solution was added, and separated into an aqueous layer and an ethyl acetate layer using a separatory funnel. The separated ethyl acetate layer was concentrated and dried with an evaporator set at 60 ° C. to obtain 1.01 g of an ethyl acetate fraction as an extraction component of Example 2.

(Example 3) Acquisition of extracted components by ultrafiltration membrane (1)
The extraction component of Example 2 was dissolved in ultrapure water and adjusted to a concentration of 10 mg / mL, and an ultrafiltration membrane with a molecular weight cut off of 3000 (manufactured by Nihon Millipore, ultrafiltration disc, Ultracell, PL, regenerated) Ultrafiltration was performed using an ultrafiltration apparatus (Nippon Millipore, Amicon Stirring Cell Model 18400) equipped with cellulose (3000NMWL). The fraction with a molecular weight of 3000 or less was collected and freeze-dried to obtain 0.97 g of a fraction with a molecular weight of 3000 or less as an extraction component of Example 3.

(Anti-ice nuclear activity measurement)
The concentration of each sample was 1 mg / mL. Anti-ice nuclear activity was measured by Vali's droplet freezing method. The principle of Vali's droplet freezing method is that an aluminum film is placed on a copper plate, and 10 μL of each sample and blank sample are dropped on the surface of 30 points, and the temperature is lowered at a rate of 1.0 ° C. per minute. Let T50 be the temperature at which 50% of the 30 droplets freeze.

  Assuming that the temperature at the time of freezing the droplet of each sample is Sample T50 and the temperature at the time of freezing the droplet of the blank sample is Blank T50, the anti-ice nucleus activity value ΔT50 (° C.) is ΔT50 (° C.) = Blank T50−Sample T50. Desired. Each sample is obtained by adding silver iodide to the extracted components of Examples 1 to 3, and the blank sample is composed only of silver iodide.

  Before measuring the anti-ice nucleus activity by the Vali droplet freezing method as described above, it is necessary to add each sample to the phosphate buffer as described later. In this example, 150 mM potassium phosphate buffer (pH 7.0) was used as the phosphate buffer. The potassium phosphate buffer was sterilized in advance by passing through a 0.2 μm filter (Cellulose Acetate 0.2 μm, manufactured by ADVANTEC).

  The principle of Vali's droplet freezing method is as described above. In this embodiment, a micro water droplet freezing measuring device is used, and as such a micro water droplet freezing measuring device, Mitsuwa Model K-1 (manufactured by Yamamoto Technical Co., Ltd.) is used. Using.

And each extraction component of Examples 1-3 was dissolved in ultrapure water, adjusted to a concentration of 1 mg / mL, passed through a 0.2 μm filter (ADVANTEC, Cellulose Acetate 0.2 μm), and then this solution 100 μL was added to 1 mL of a blank prepared by adding silver iodide to potassium phosphate buffer to a concentration of 1 mg / mL, and the anti-ice nucleus activity value was measured. The results are shown in Table 1.

  As shown in Table 1, the anti-ice nucleus activity values of the extracted components of Examples 1, 2, and 3 are 1.4 ° C., 3.6 ° C., and 4.3 ° C., respectively. It was found that the anti-ice nucleus activity value was increased by extraction, and the anti-ice nucleus activity value was further increased by extraction with ethyl acetate and fractionation into fractions having a molecular weight of 3000 or less. In addition, the anti-ice nucleus activity value of the water tank after fractionating the ethyl acetate layer in Example 2 was 0 ° C.

(Example 4) Acquisition of extraction component by ultrafiltration membrane (2)
The extraction component (adsorbed fraction) obtained in the same manner as in Example 1 was dissolved in ultrapure water and adjusted so that the dry weight concentration was 1 mg / mL. Similarly, ultrafiltration was performed using an ultrafiltration membrane having a fractional molecular weight of 3000. The fraction having a molecular weight of 3000 or less thus fractionated was collected and lyophilized to obtain 4.37 g of a fraction having a molecular weight of 3000 or less as an extraction component of Example 4. About the obtained extraction component, the anti-ice nucleus activity value was measured like the above. The results are shown in Table 2.

  As shown in Table 2, the anti-ice nucleus activity value was 2.1 ° C. From this, the case of obtaining an ethyl acetate fraction extracted with ethyl acetate from the adsorbed fraction (Example 2) is more effective than the case of obtaining a fraction having a molecular weight of 3000 or less as it is from the adsorbed fraction (Example 4). It was found that it was effective to extract with ethyl acetate after the nuclear activity value increased and the adsorbed fraction was obtained. Moreover, it was suggested that the component soluble in ethyl acetate shows high anti-ice nucleus activity.

(Examples 5 to 7) Acquisition of extraction components by ultrafiltration membrane (3)
The extraction component (adsorbed fraction) obtained in the same manner as in Example 1 was dissolved in ultrapure water and adjusted to a dry weight concentration of 1 mg / mL. I made a picture. Sephadex LH-20 (manufactured by GE Healthcare Japan) was used as the carrier, and ultrapure water was used as the eluent. The column size was 15 mm × 100 mm, and the flow rate was 0.3 ml / min. As gel filtration chromatography, flash chromatograph SYS16020 (manufactured by Tokyo Science Instruments Co., Ltd.) was used.

  The obtained chart is shown in FIG. In FIG. 1, the horizontal axis represents the fraction number, and the vertical axis represents the absorbance at 275 nm (A275). According to FIG. 1, three peaks were observed (peak 1, peak 2, peak 3 in FIG. 1), and ultraviolet absorption spectra were measured for the fractions 37, 51 and 70 indicating the peak vertices of peaks 1 to 3. The obtained ultraviolet absorption spectrum charts are shown in FIGS. 2 (a) to 2 (c).

  As shown in FIGS. 2 (a) to (c), a peak was observed in the vicinity of a wavelength of 215 nm for each fraction, suggesting that a peptide bond was present in each fraction. Further, as shown in FIG. 2 (c), a shoulder peak was observed in the vicinity of a wavelength of 275 nm in the fraction showing peak 3, suggesting the possibility of containing an aromatic.

Then, the three fractions 37, 51, and 70 showing the peaks 1, 2, and 3 were freeze-dried in the same manner as in Example 3 to obtain the extracted components of Examples 5, 6, and 7, and the same as above. The anti-ice nucleus activity value was measured. The results are shown in Table 3.

  As shown in Table 3, the anti-ice nucleus activity values of the extracted components of Examples 5, 6, and 7 are 1.8 ° C, 2.0 ° C, and 5.3 ° C, respectively. The anti-ice nucleus activity value was the largest. As a result, it was suggested that the component having a lower molecular weight exhibits higher anti-ice nucleus activity.

(Examples 8 to 13) Measurement of anti-ice nuclear activity values for various components extracted from Japanese sake As shown in Table 4, Daiginjo sake, Ginjo sake, Junmai sake, Honjozo, non-filtered raw sake, ordinary sake 6 types of sake were used.

  Here, Daiginjo Sake is made from white rice, rice bran, water, and brewed alcohol with a rice polishing ratio of 50% or less, and Ginjo Sake has white rice, rice bran and water with a rice polishing ratio of 60% or less, Alternatively, these are produced using brewed alcohol as a raw material. Pure rice sake is produced using white rice, rice bran and water as raw materials, and this brewed sake is produced from white rice and rice bran with a rice polishing ratio of 70% or less. Brewed alcohol and water are used as raw materials, and unfiltered raw sake is not filtered or heat-treated after production, and ordinary sake is the above-mentioned Daiginjo sake, Ginjo sake, etc. It is a sake other than the specially named sake, and is a commonly distributed sake.

From these six types of sake, adsorbed fractions were obtained in the same manner as in Example 1, and fractions having a molecular weight of 3000 or less were obtained from the ethyl acetate fraction by ultrafiltration in the same manner as in Example 3. The anti-ice nucleus activity value was measured in the same manner as described above. The results are shown in Table 4.

  As shown in Table 4, in any sake, the extracted component obtained by adsorption fractionation with a styrene-divinylbenzene synthetic adsorbent exhibits anti-ice nucleus activity, and this is extracted with ethyl acetate, and the molecular weight It was confirmed that the extracted component obtained by fractionation into a fraction of 3000 or less showed higher anti-ice nucleus activity. In particular, it was confirmed that the extracted components of Example 8 extracted from Daiginjo Sake, Example 10 extracted from Junmai Sake, and Example 12 extracted from unfiltered raw sake had high ice nuclei activity.

  In addition, the anti-ice nucleus activity value of the extract components of Example 9, Example 11, and Example 13 was low because brewing alcohol was added to Ginjo Sake, Honjo Sake and Ordinary Sake in addition to rice and koji. It is speculated that this is due in part to the fact that the absolute amount of ice core active substances contained in sake is small.

  On the other hand, in view of the fact that the ice nucleation activity value of the extracted component of Example 8 was the highest, Daiginjo sake has a high ratio of rice shaving (rice polishing ratio), so it is not outside the rice with a large amount of protein, It was suggested that the heart white part in the center of rice contributed to ice core activity.

Claims (6)

The components contained in sake are adsorbed on a styrene-divinylbenzene synthetic adsorbent, the adsorbate adsorbed on the synthetic adsorbent is extracted with ethyl acetate, and the extract extracted with ethyl acetate is extracted from sake. anti ice nucleation active agent characterized by containing as. The components contained in sake are adsorbed on a styrene-divinylbenzene synthetic adsorbent, and the adsorbate adsorbed on the synthetic adsorbent is fractionated into a fraction having a molecular weight of 3000 or less by an ultrafiltration membrane, and the fraction having a molecular weight of 3000 or less is obtained. anti ice nucleation active agent characterized by containing as an extract component from sake.   The anti-ice nucleus activator according to claim 1, wherein the extract is fractionated into a fraction having a molecular weight of 3000 or less by an ultrafiltration membrane, and the fraction having a molecular weight of 3000 or less is contained as an extraction component from sake. . The method includes an adsorption step of adsorbing a component of sake to a styrene-divinylbenzene synthetic adsorbent and an extraction step of extracting an adsorbate adsorbed on the synthetic adsorbent with ethyl acetate, and the extracted component is extracted with ethyl acetate. A method for producing an anti-ice nucleation activator characterized in that an extract is contained as an extract component from sake. An adsorption step of adsorbing the components of sake on the styrene-divinylbenzene synthetic adsorbent, and a fractionation step of fractionating the adsorbate adsorbed on the synthetic adsorbent into a fraction having a molecular weight of 3000 or less by an ultrafiltration membrane. A method for producing an anti-ice nucleation activator comprising: containing a fraction having a molecular weight of 3000 or less as an extraction component from sake. The method further comprises a fractionation step of fractionating the extract extracted with ethyl acetate into a fraction having a molecular weight of 3000 or less by an ultrafiltration membrane, and the fraction having a molecular weight of 3000 or less is contained as an extraction component from sake. The manufacturing method of the anti-ice nucleus active agent of Claim 4.

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