JP5563985B2 - Phenylisonicotinic acid derivative and pharmaceutical use thereof - Google Patents

Description

  The present invention relates to phenylisonicotinic acid derivatives useful as pharmaceuticals.

  More specifically, the present invention relates to phenylisonicotinic acid derivatives or prosthetics thereof having xanthine oxidase and URAT1 (uric acid transporter 1) inhibitory activity and useful as preventive or therapeutic agents for diseases caused by abnormal serum uric acid levels. It relates to a drug or a pharmacologically acceptable salt thereof.

  Uric acid is the end product of purine metabolism in humans. In many mammals, unlike humans, uric acid is further decomposed into allantoin by the urate oxidase (uricase) in the liver and excreted from the kidney. The main route of uric acid excretion in humans is the kidney, about 2/3 is excreted in the urine and the rest is excreted from the stool. Hyperuricemia occurs due to excessive uric acid production or decreased uric acid excretion. Hyperuricemia is classified into an excessive uric acid production type, a reduced uric acid excretion type, and a mixed type thereof. This classification of hyperuricemia is clinically important, and therapeutic drugs in each classification are selected in consideration of reducing the side effects of the therapeutic drugs (see, for example, Non-Patent Document 1).

In uric acid-producing hyperuricemia, urinary uric acid excretion is increased, and if urinary uric acid excretion is further increased by the use of a uric acid excretion-promoting drug, urinary calculi may be combined. Therefore, in principle, allopurinol, which is a uric acid production inhibitor (or uric acid synthesis inhibitor, hereinafter referred to as “uric acid production inhibitor”), is used for the uric acid production excessive type.
Uric acid is finally produced from xenotin oxidized by xanthine oxidase from diet-derived and endogenously produced purines. Allopurinol is the only uric acid production inhibitor developed as a xanthine oxidase inhibitor and used in the medical field. However, allopurinol has been reported to be effective against hyperuricemia and various diseases resulting from it, but on the other hand, poisoning syndrome (hypersensitivity vasculitis), Stevens-Johnson syndrome, exfoliative dermatitis, aplasticity Serious side effects such as anemia and liver dysfunction have also been reported (for example, see Non-Patent Document 2). As one of the causes, it has been pointed out that allopurinol has a nucleic acid-like structure and inhibits the pyrimidine metabolic pathway (see, for example, Non-Patent Document 3).

  On the other hand, in uric acid excretion-type hyperuricemia, the excretion of uric acid is decreased, and when allopurinol, which is metabolized by oxypurinol excreted from the kidney by the same mechanism as uric acid, is used, the excretion of oxypurinol is also reduced. It has been reported that the frequency of liver damage increases and the frequency of liver damage increases (see, for example, Non-Patent Document 4). Therefore, in principle, uric acid excretion promoting agents such as probenecid and benzbromarone are used for the urate excretion-reducing type. However, these uric acid excretion promoting agents also exhibit side effects such as gastrointestinal disorders and urinary calculi. In particular, benzbromarone is known to cause fulminant hepatitis in patients with idiosyncratic constitution (see, for example, Non-Patent Documents 5 and 6).

  As described above, it is said that both existing uric acid production inhibitors and uric acid excretion promoters have limitations on use and severe side effects on patients, and development of easy-to-use therapeutic agents such as hyperuricemia is eagerly desired.

  Uric acid is mainly excreted from the kidney, but the dynamics of uric acid in the kidney have been studied by experiments using brush border membrane vesicles (BBMV) prepared from the renal cortex (for example, Non-Patent Document 7 and 8). It has been clarified that uric acid freely passes through the glomeruli in humans in humans, and there is a mechanism of reabsorption and secretion of uric acid in the proximal tubule (see, for example, Non-Patent Document 9).

  In recent years, a gene (SLC22A12) encoding a human renal urate transporter has been identified (see, for example, Non-Patent Document 10). A transporter (urate transporter 1, hereinafter referred to as “URAT1”) encoded by this gene is a 12-transmembrane molecule belonging to the OAT family. URAT1 specifically expressed mRNA in the kidney, and was further localized on the proximal tubular lumen side in human kidney tissue sections. Experiments with the Xenopus oocyte expression system showed uric acid uptake via URAT1. Furthermore, its uptake of uric acid is transported by exchange with organic anions such as lactic acid, pyrazinecarboxylic acid (PZA) and nicotinic acid, and urate uptake through URAT1 is inhibited by probenecid and benzbromarone, which are uric acid excretion promoters. It became clear that. Therefore, it was strongly suggested to be an urate / anion exchanger, which was expected by experiments using membrane vesicles. That is, it was revealed that URAT1 is a transporter that plays an important role in uric acid reabsorption in the kidney (see, for example, Non-Patent Document 10).

  In addition, the relationship between URAT1 and diseases has also been clarified. Idiopathic renal hypouricemia is a disease in which uric acid excretion is increased due to abnormal uric acid dynamics in the kidney and serum uric acid level is low. In this disease, it is known that there are many complications of urinary calculi and acute renal failure after exercise. URAT1 was identified as a causative gene of this renal hypouricemia (for example, refer nonpatent literature 10). The above also strongly suggests that URAT1 is involved in the regulation of blood uric acid levels.

  Therefore, substances having a URAT1 inhibitory activity are therapeutic agents for diseases involving high blood uric acid levels, that is, hyperuricemia, gouty nodules, gout arthritis, renal disorders due to hyperuricemia, urolithiasis and the like. It is useful as a preventive drug.

  In the treatment of hyperuricemia, it was reported that the combined use of allopurinol, a uric acid production inhibitor, and a drug that promotes excretion of uric acid, showed a stronger decrease in serum uric acid level compared to allopurinol alone. (For example, see Non-Patent Documents 11 and 12). That is, when the effect of the conventional treatment with a single agent is not sufficient, a higher therapeutic effect can be expected by the combined use of a uric acid production inhibitor and a uric acid excretion promoter. Furthermore, for uric acid excretion-type hyperuricemia, urinary uric acid excretion can be reduced by lowering the blood uric acid level. Is thought to be reduced. Moreover, a high therapeutic effect can be expected for mixed hyperuricemia. As described above, a drug having both a uric acid production inhibitory action and a uric acid excretion promoting action is expected to be a very useful preventive or therapeutic agent for hyperuricemia and the like.

  As a compound having both xanthine oxidase inhibitory action and URAT1 inhibitory action, natural product morin is known (see Non-Patent Document 13). Biaryl or diaryl ether compounds are known as compounds having a uric acid excretion promoting action (see Patent Document 1).

As a xanthine oxidase inhibitor, certain 2-phenylisonicotinic acid derivatives (for example, see Patent Documents 2 to 4) and 2-phenylnicotinic acid derivatives (for example, see Patent Document 5) are known. However, the 2-phenyl-5-hydroxyisonicotinic acid compound of the present invention is not disclosed at all. In addition, it has been reported that certain 2-phenyl-5-hydroxyisonicotinic acid derivatives have anti-inflammatory action and the like (see Patent Document 6 and Non-Patent Document 14), but the compounds of the present invention are disclosed. Not. None of the above references describe or suggest that the compounds of the present invention have a URAT1 inhibitory action.
JP 2000-001431 A International Publication No. 2006/022374 Pamphlet International Publication No. 2007/043457 Pamphlet International Publication No. 2007/099743 Pamphlet International Publication No. 2008/072658 Pamphlet US Pat. No. 3,703,582 Tatsuo Taniguchi and one other, Modern Physician, 2004, Vol. 24, No. 8, pp. 1309-1312 Kazuhide Kanno and two others, Japanese Clinical, 2003, Volume 61, Special Number 1, p.197-201 Hideki Horiuchi and 6 others, Life Science, 2000, 66, 21, p.2051-2070 Toru Yamanaka and two others, hyperuricemia and gout, published by Medical Review, 1994, Vol. 2, No. 1, p.103-111 Robert A Terkeltaub, N. Engl. J. Med., 2003, 349, p. 1647-1655 Ming-Han H. Lee and 3 others, Drug Safety, 2008, 31, p.643-665 Francoise Roch-Ramel and two others, Am. J. Physiol., 1994, 266 (Renal Fluid Electrolyte Physiol. 35), F797-F805 Francoise Roch-Ramel and two others, J. Pharmacol. Exp. Ther., 1997, 280, pp. 839-845 Gim Gee Teng and two others, Drugs, 2006, 66, p. 1547-1563 Atsushi Enomoto and 18 others, Nature, 2002, 417, pp. 447-452 S Takahashi and 5 others, Ann. Rheum. Dis., 2003, 62, 572-575 MDFeher and 4 others, Rheumatology, 2003, 42, pp.321-325 Zhifeng Yu and two others, J. Pharmacol. Exp. Ther., 2006, 316, pp. 169-175 GL Walford and two others, Journal of Medicinal Chemistry, 1971, Vol. 14, No. 4, p. 339-344

  An object of the present invention is to provide a preventive or therapeutic agent for diseases caused by abnormal serum uric acid levels, which has a uric acid production inhibitory effect.

  As a result of intensive studies to solve the above problems, the present inventors have shown that phenylisonicotinic acid derivatives represented by the following formula (I) exhibit excellent xanthine oxidase and URAT1 inhibitory activities, and have remarkable serum uric acid levels. Therefore, the present inventors have found that it can be a novel preventive or therapeutic agent for diseases caused by abnormal serum uric acid levels, and has led to the present invention.

〔式中、
Ｒ^１は、シアノ、トリフルオロメチル又は塩素原子；
Ｒ^２は、水素原子、フッ素原子、塩素原子、水酸基、アミノ、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、ヒドロキシＣ_１−６アルキル、Ｃ_１−６アルコキシＣ_１−６アルキル、フッ素化Ｃ_１−６アルコキシＣ_１−６アルキル、Ｃ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシ、ヒドロキシＣ_１−６アルコキシ、Ｃ_１−６アルコキシＣ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシＣ_１−６アルコキシ、Ｃ_１−６アルキルチオ、フッ素化Ｃ_１−６アルキルチオ、Ｃ_２−６アルケニル、モノ（ジ）Ｃ_１−６アルキルアミノ、ヒドロキシ（モノ（ジ）Ｃ_１−６アルキルアミノ）、Ｃ_１−６アルコキシＣ_１−６アルキルアミノ、Ｃ_１−６アルコキシＣ_１−６アルキル（Ｃ_１−６アルキル）アミノ、Ｃ_３−８シクロアルキル、３〜８員環ヘテロシクロアルキル、Ｃ_３−８シクロアルキルＣ_１−６アルキル、３〜８員環ヘテロシクロアルキルＣ_１−６アルキル、Ｃ_３−８シクロアルキルＣ_１−６アルコキシ、３〜８員環ヘテロシクロアルキルＣ_１−６アルコキシ、Ｃ_３−８シクロアルコキシ又は３〜８員環ヘテロシクロアルコキシ（ここで、当該Ｃ_３−８シクロアルキル、３〜８員環ヘテロシクロアルキル、Ｃ_３−８シクロアルコキシ及び３〜８員環ヘテロシクロアルコキシ、並びにＣ_３−８シクロアルキルＣ_１−６アルキル、３〜８員環ヘテロシクロアルキルＣ_１−６アルキル、Ｃ_３−８シクロアルキルＣ_１−６アルコキシ及び３〜８員環ヘテロシクロアルキルＣ_１−６アルコキシにおける３〜８員環ヘテロシクロアルキル及びＣ_３−８シクロアルコキシ部分は、それぞれ置換基群αから選択される同一又は異なる基を１〜３個有していてもよい）；
置換基群αは、ハロゲン原子、水酸基、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、ヒドロキシＣ_１−６アルキル、Ｃ_１−６アルコキシＣ_１−６アルキル、フッ素化Ｃ_１−６アルコキシＣ_１−６アルキル、Ｃ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシ、ヒドロキシＣ_１−６アルコキシ、Ｃ_１−６アルコキシＣ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシＣ_１−６アルコキシ；
Ｒ^３は、Ｗ又は−Ｘ−Ｙ−Ｚで表される基；
Ｗは、オクタヒドロシクロペンタ［ｃ］ピロリル、オクタヒドロイソインドリル、２−オキサ−５−アザビシクロ［２．２．１］ヘプタ−５−イル、Ｃ_３−８シクロアルキルアミノ、又はそれぞれ、ハロゲン原子、水酸基、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、ヒドロキシＣ_１−６アルキル、Ｃ_１−６アルコキシＣ_１−６アルキル、フッ素化Ｃ_１−６アルコキシＣ_１−６アルキル、Ｃ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシ、ヒドロキシＣ_１−６アルコキシ、Ｃ_１−６アルコキシＣ_１−６アルコキシ及びフッ素化Ｃ_１−６アルコキシＣ_１−６アルコキシからなる群から選択される同一又は異なる基を１〜３個有していてもよいＣ_３−８シクロアルキル、３〜８員環ヘテロシクロアルキル、Ｃ_３−８シクロアルコキシ、３〜８員環ヘテロシクロアルコキシ、Ｃ_６−１０アリールオキシ又は５又は６員環ヘテロアリールオキシ；
Ｘは、単結合、−Ｎ（Ｒ^５）−、−Ｏ−又は−Ｓ−（Ｒ^５は、水素原子又はＣ_１−６アルキル）；
Ｙは、Ｃ_１−６アルキレン又はＣ_２−６アルケニレン；
Ｚは、水素原子；水酸基；Ｃ_１−６アルコキシ；フッ素化Ｃ_１−６アルコキシ；チオール；Ｃ_１−６アルキルチオ；フッ素化Ｃ_１−６アルキルチオ；又はそれぞれハロゲン原子、水酸基、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、Ｃ_１−６アルコキシ及びフッ素化Ｃ_１−６アルコキシからなる群から選択される同一又は異なる基を１〜３個有していてもよいＣ_３−８シクロアルキル、３〜８員環ヘテロシクロアルキル、Ｃ_６−１０アリールもしくは５又は６員環ヘテロアリール（但し、Ｃ_６−１０アリールが環内の隣り合った２つの原子上にそれぞれＣ_１−６アルコキシを有するときは、これらが一緒になって−ＯＣＨ_２Ｏ−、−Ｏ（ＣＨ_２）_２Ｏ−、−ＯＣＦ_２Ｏ−又は−Ｏ（ＣＦ_２）_２Ｏ−を形成してもよい）；である〕で表されるフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔２〕 Ｒ^３が、Ｗ^０又は−Ｘ−Ｙ−Ｚで表される基（Ｗ^０は、それぞれ、ハロゲン原子、水酸基、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、ヒドロキシＣ_１−６アルキル、Ｃ_１−６アルコキシＣ_１−６アルキル、フッ素化Ｃ_１−６アルコキシＣ_１−６アルキル、Ｃ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシ、ヒドロキシＣ_１−６アルコキシ、Ｃ_１−６アルコキシＣ_１−６アルコキシ及びフッ素化Ｃ_１−６アルコキシＣ_１−６アルコキシからなる群から選択される同一又は異なる基を１〜３個有していてもよいＣ_３−８シクロアルキル、３〜８員環ヘテロシクロアルキル、Ｃ_３−８シクロアルコキシ、３〜８員環ヘテロシクロアルコキシ、Ｃ_６−１０アリールオキシ又は５又は６員環ヘテロアリールオキシであり、Ｘ、Ｙ及びＺは前記と同じ意味である）である、前記〔１〕記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔３〕 Ｒ^１がシアノである前記〔１〕又は〔２〕記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；That is, the present invention
[1] Formula (I)

[Where,
R ¹ is a cyano, trifluoromethyl or chlorine atom;
R ² represents a hydrogen atom, a fluorine atom, a chlorine atom, a hydroxyl group, amino, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, fluorine C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy, fluorinated C _1-6 Alkoxy C _1-6 alkoxy, C _1-6 alkylthio, fluorinated C _1-6 alkylthio, C _2-6 alkenyl, mono (di) C _1-6 alkylamino, hydroxy (mono (di) C _1-6 alkylamino _{), C 1-6} alkoxy _{C 1-6 alkylamino, C 1-6} alkoxy _{C 1-6} alkyl _{(C 1-6} alkyl) _{amino, C 3-8} cycloalkyl , 3-8 membered _{heterocycloalkyl, C 3-8} cycloalkyl _{a C 1-6} alkyl, 3-8 membered heterocycloalkyl _{C 1-6 alkyl, C 3-8} cycloalkyl _{a C 1-6} alkoxy, 3 8-membered heterocycloalkyl C _1-6 alkoxy, C _3-8 cycloalkoxy or 3-8 membered heterocycloalkoxy (wherein the C _3-8 cycloalkyl, 3-8 membered heterocycloalkyl, C _{3 -8} cycloalkoxy and 3-8 membered heterocycloalkoxy, and C _3-8 cycloalkyl C _1-6 alkyl, 3-8 membered heterocycloalkyl C _1-6 alkyl, C _3-8 cycloalkyl C _{1- 6} alkoxy and 3-8 membered heterocycloalkyl _{C 1-6} 3-8 membered heterocycloalkyl and _{C 3-8} Shikuroaruko in alkoxy Shi moiety may have 1 to 3 identical or different groups selected from α each substituent group);
Substituent group α is halogen atom, hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy, fluorinated C _1-6 alkoxy C _1-6 alkoxy ;
R ³ represents a group represented by W or —X—Y—Z;
W is octahydrocyclopenta [c] pyrrolyl, octahydroisoindolyl, 2-oxa-5-azabicyclo [2.2.1] hept-5-yl, C _3-8 cycloalkylamino, or halogen, respectively Atom, hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C Selected from the group consisting of _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy C _1-6 alkoxy identical or different radicals from 1 to 3 have good _{C 3-8} also be cycloalkyl, 3-8 membered _{heterocycloalkyl, C 3-8} cycloalk Alkoxy, 3-8 membered heterocycloalkyl _{alkoxy, C 6-10} aryloxy, or 5 or 6-membered heteroaryloxy;
X is a single bond, —N (R ⁵ ) —, —O— or —S— (R ⁵ is a hydrogen atom or C _1-6 alkyl);
Y is C _1-6 alkylene or C _2-6 alkenylene;
Z is hydrogen atom; hydroxyl group; C _1-6 alkoxy; fluorinated C _1-6 alkoxy; thiol; C _1-6 alkylthio; fluorinated C _1-6 alkylthio; or halogen atom, hydroxyl group, C _1-6 alkyl, respectively. C _3-8 cycloalkyl optionally having 1 to 3 identical or different groups selected from the group consisting of fluorinated C _1-6 alkyl, C _1-6 alkoxy and fluorinated C _1-6 alkoxy , 3-8 membered heterocycloalkyl, C _6-10 aryl or 5 or 6 membered heteroaryl (wherein C _6-10 aryl is each substituted with C _1-6 alkoxy on two adjacent atoms in the ring, when having the, -OCH ₂ O these together _{-, - O (CH 2)} 2 O -, - OCF 2 O- or -O _{(CF 2)} may form a 2 O-); Phenyl isonicotinic acid derivative represented by certain] or a prodrug thereof, or a pharmaceutically acceptable salt thereof;
[2] R ³ is a group represented by W ⁰ or —X—Y—Z (W ⁰ is a halogen atom, a hydroxyl group, C _1-6 alkyl, a fluorinated C _1-6 alkyl, or a hydroxy C _{1, respectively. -6} alkyl, C _1-6 alkoxy C _1-6 alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C C _3-8 cycloalkyl optionally having 1 to 3 identical or different groups selected from the group consisting of _1-6 alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy C _1-6 alkoxy , 3-8 membered _{heterocycloalkyl, C 3-8} cycloalkoxy, a 3-8 membered heterocycloalkyl _{alkoxy, C 6-10} aryloxy, or 5 or 6 membered heteroaryloxy, , Y and Z have the same meaning and is) and the said [1] phenyl isonicotinic acid derivative or a prodrug thereof, or a pharmaceutically acceptable salt thereof according;
[3] The phenylisonicotinic acid derivative according to the above [1] or [2], wherein R ¹ is cyano, or a prodrug thereof, or a pharmacologically acceptable salt thereof;

〔式中、Ｗ^１は、結合部位が窒素原子であり、ハロゲン原子、水酸基、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、ヒドロキシＣ_１−６アルキル、Ｃ_１−６アルコキシＣ_１−６アルキル、フッ素化Ｃ_１−６アルコキシＣ_１−６アルキル、Ｃ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシ、ヒドロキシＣ_１−６アルコキシ、Ｃ_１−６アルコキシＣ_１−６アルコキシ及びフッ素化Ｃ_１−６アルコキシＣ_１−６アルコキシからなる群から選択される同一又は異なる基を１〜３個有していてもよい３〜８員環ヘテロシクロアルキルである〕で表される前記〔１〕〜〔３〕の何れかに記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔５〕 Ｗ^１が、それぞれ、ハロゲン原子、水酸基、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、ヒドロキシＣ_１−６アルキル、Ｃ_１−６アルコキシＣ_１−６アルキル、フッ素化Ｃ_１−６アルコキシＣ_１−６アルキル、Ｃ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシ、ヒドロキシＣ_１−６アルコキシ、Ｃ_１−６アルコキシＣ_１−６アルコキシ及びフッ素化Ｃ_１−６アルコキシＣ_１−６アルコキシからなる群から選択される同一又は異なる基を１〜３個有していてもよい、ピロリジン、ピペリジン、アゼパンもしくはモルホリンである、前記〔４〕記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔６〕 Ｗ^１が、下記式（ＩＩａ）

〔式中、Ｒ^３ａは、水素原子、ハロゲン原子、水酸基、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、ヒドロキシＣ_１−６アルキル、Ｃ_１−６アルコキシＣ_１−６アルキル、フッ素化Ｃ_１−６アルコキシＣ_１−６アルキル、Ｃ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシ、ヒドロキシＣ_１−６アルコキシ、Ｃ_１−６アルコキシＣ_１−６アルコキシ又はフッ素化Ｃ_１−６アルコキシＣ_１−６アルコキシ；
Ｒ^３ｂは、水素原子又はフッ素原子；である〕で表されるピペリジンである、前記〔４〕記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔７〕 Ｒ^３ａ及びＲ^３ｂがいずれもフッ素原子である、前記〔６〕記載のビアリールイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔８〕 Ｗ^１が、それぞれ置換基を有していないモルホリン又は１，４−オキサゼパンである、前記〔４〕記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；[4] Formula (Ia)

[Wherein, W ¹ is a nitrogen atom at the bonding site, a halogen atom, a hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _{1- 6} alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy is a 3- to 8-membered heterocycloalkyl which may have 1 to 3 identical or different groups selected from the group consisting of C _1-6 alkoxy]. ] The phenylisonicotinic acid derivative or prodrug thereof or the pharmacologically acceptable salt thereof according to any one of [3] to [3];
[5] W ¹ is a halogen atom, a hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, or fluorinated C _{1, respectively. -6} alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy C ₁ A phenylisonicotinic acid derivative according to the above [4] or a pro thereof, which is pyrrolidine, piperidine, azepane or morpholine, which may have 1 to 3 identical or different groups selected from the group consisting of _-6 alkoxy. A drug or a pharmacologically acceptable salt thereof;
[6] W ¹ represents the following formula (IIa)

[Wherein, R ^3a represents a hydrogen atom, a halogen atom, a hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy or fluorinated C _1-6 alkoxy C _1-6 alkoxy;
R ^3b is a hydrogen atom or a fluorine atom; and the phenylisonicotinic acid derivative or prodrug thereof or pharmacologically acceptable salt thereof according to the above [4], which is a piperidine represented by
[7] The biarylisonicotinic acid derivative or the prodrug thereof or the pharmacologically acceptable salt thereof according to [6] above, wherein R ^3a and R ^3b are both fluorine atoms;
[8] The phenylisonicotinic acid derivative or prodrug thereof or pharmacologically acceptable salt thereof according to [4], wherein W ¹ is morpholine or 1,4-oxazepane each having no substituent. ;

〔式中、Ｗ^２は、結合部位が炭素原子であり、それぞれハロゲン原子、水酸基、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、ヒドロキシＣ_１−６アルキル、Ｃ_１−６アルコキシＣ_１−６アルキル、フッ素化Ｃ_１−６アルコキシＣ_１−６アルキル、Ｃ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシ、ヒドロキシＣ_１−６アルコキシ、Ｃ_１−６アルコキシＣ_１−６アルコキシ及びフッ素化Ｃ_１−６アルコキシＣ_１−６アルコキシからなる群から選択される同一又は異なる基を１〜３個有していてもよいＣ_３−８シクロアルキル又は３〜８員環ヘテロシクロアルキルである〕で表される前記〔１〕〜〔３〕の何れかに記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔１０〕 Ｗ^２が、それぞれ、ハロゲン原子、水酸基、Ｃ_１−６アルキル、フッ素化Ｃ_１−６アルキル、ヒドロキシＣ_１−６アルキル、Ｃ_１−６アルコキシＣ_１−６アルキル、フッ素化Ｃ_１−６アルコキシＣ_１−６アルキル、Ｃ_１−６アルコキシ、フッ素化Ｃ_１−６アルコキシ、ヒドロキシＣ_１−６アルコキシ、Ｃ_１−６アルコキシＣ_１−６アルコキシ及びフッ素化Ｃ_１−６アルコキシＣ_１−６アルコキシからなる群から選択される同一又は異なる基を１〜３個有していてもよい、シクロプロピル、シクロペンチル、シクロへキシルもしくはテトラヒドロピラニルである、前記〔９記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔１１〕 Ｗ^２が、置換基を有していないシクロプロピルである、前記〔９〕記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔１２〕 Ｘが単結合；ＹがＣ_１−６アルキレンである、前記〔１〕〜〔３〕の何れかに記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔１３〕 Ｘが−Ｎ（Ｒ^５）−又は−Ｏ−（Ｒ^５は水素原子又はメチル基）である、前記〔１〕〜〔３〕の何れかに記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔１４〕 ＹがＣ_１−６アルキレンである、前記〔１３〕記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔１５〕 Ｚが水素原子である、前記〔１２〕〜〔１４〕の何れかに記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔１６〕 Ｒ^２が水素原子である前記〔１〕〜〔１５〕の何れかに記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔１７〕 ＵＲＡＴ１阻害薬である、前記〔１〕〜〔１６〕の何れかに記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩；
〔１８〕 前記〔１〕〜〔１６〕の何れかに記載のフェニルイソニコチン酸誘導体もしくはそのプロドラッグ又はその薬理学的に許容される塩を有効成分として含有する医薬組成物；
〔１９〕 高尿酸血症、痛風結節、痛風関節炎、高尿酸血症による腎障害及び尿路結石から選択される疾患の予防又は治療用である、前記〔１８〕記載の医薬組成物。
〔２０〕 高尿酸血症の予防又は治療用である、前記〔１９〕記載の医薬組成物。
〔２１〕 血漿尿酸値低下薬である、前記〔１８〕記載の医薬組成物；等に関するものである。[9] Formula (Ib)

[Wherein, W ² is a carbon atom at the bonding site, and is a halogen atom, hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _{1, respectively. -6} alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorine C _1-6 alkoxy is C _3-8 cycloalkyl or 3-8 membered heterocycloalkyl optionally having 1 to 3 identical or different groups selected from the group consisting of C _1-6 alkoxy ] The phenylisonicotinic acid derivative or the prodrug thereof or the pharmacologically acceptable salt thereof according to any one of the above [1] to [3];
[10] W ² is a halogen atom, hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, or fluorinated C _{1, respectively. -6} alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy C ₁ The phenylisonicotinic acid according to [9], which is cyclopropyl, cyclopentyl, cyclohexyl or tetrahydropyranyl, which may have 1 to 3 identical or different groups selected from the group consisting of _-6 alkoxy A derivative or a prodrug thereof or a pharmacologically acceptable salt thereof;
[11] The phenylisonicotinic acid derivative or prodrug thereof or pharmacologically acceptable salt thereof according to [9] above, wherein W ² is cyclopropyl having no substituent;
[12] The phenylisonicotinic acid derivative or prodrug thereof or pharmacologically acceptable thereof according to any one of [1] to [3], wherein X is a single bond; Y is C _1-6 alkylene. salt;
[13] The phenylisonicotinic acid derivative according to any one of the above [1] to [3], wherein X is —N (R ⁵ ) — or —O— (R ⁵ is a hydrogen atom or a methyl group) or a derivative thereof A prodrug or a pharmacologically acceptable salt thereof;
[14] The phenylisonicotinic acid derivative or the prodrug thereof or the pharmaceutically acceptable salt thereof according to [13], wherein Y is C _1-6 alkylene;
[15] The phenylisonicotinic acid derivative or prodrug thereof or pharmacologically acceptable salt thereof according to any one of [12] to [14], wherein Z is a hydrogen atom;
[16] The phenylisonicotinic acid derivative or the prodrug thereof or the pharmaceutically acceptable salt thereof according to any one of [1] to [15], wherein R ² is a hydrogen atom;
[17] A phenylisonicotinic acid derivative or a prodrug thereof or a pharmacologically acceptable salt thereof according to any one of [1] to [16], which is a URAT1 inhibitor;
[18] A pharmaceutical composition comprising the phenylisonicotinic acid derivative according to any one of [1] to [16], a prodrug thereof, or a pharmacologically acceptable salt thereof as an active ingredient;
[19] The pharmaceutical composition according to the above [18], which is used for the prevention or treatment of a disease selected from hyperuricemia, gouty nodule, gout arthritis, renal disorder due to hyperuricemia and urolithiasis.
[20] The pharmaceutical composition according to the above [19], which is for prevention or treatment of hyperuricemia.
[21] The pharmaceutical composition according to [18] above, which is a drug for lowering plasma uric acid level.

  In the present invention, each term has the following meaning unless otherwise specified.

“Halogen atom” refers to a fluorine atom, a chlorine atom, a bromine atom or an iodine atom.
“C _1-6 alkyl” refers to a linear or branched alkyl group having 1 to 6 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, etc. Is mentioned.
“C _1-6 alkylene” refers to a divalent group derived from the above C _1-6 alkyl.
“C _2-6 alkenyl” refers to a linear or branched alkenyl group having 2 to 6 carbon atoms, and examples thereof include vinyl, allyl, 1-propenyl and the like.
“C _2-6 alkenylene” refers to a divalent group derived from the above C _2-6 alkenyl.
“C _1-6 alkoxy” refers to a linear or branched alkoxy group having 1 to 6 carbon atoms, and includes methoxy, ethoxy, propoxy, isopropoxy and the like.
“Fluorinated C _1-6 alkyl” refers to the above C _1-6 alkyl substituted with 1 to 3 fluorine atoms.
“Fluorinated C _1-6 alkoxy” refers to the above C _1-6 alkoxy substituted with 1 to 3 fluorine atoms.
“Hydroxy C _1-6 alkyl” refers to the above C _1-6 alkyl substituted with 1 or 2 hydroxyl groups.
“Hydroxy C _1-6 alkoxy” refers to the above C _1-6 alkoxy substituted with one or two hydroxyl groups.
“C _1-6 alkoxy C _1-6 alkyl” refers to the above C _1-6 alkyl substituted with the above C _1-6 alkoxy.
“Fluorinated C _1-6 alkoxy C _1-6 alkyl” refers to the above C _1-6 alkoxy C _1-6 alkyl substituted with 1 to 3 fluorine atoms.
“C _1-6 alkoxy C _1-6 alkoxy” refers to the above C _1-6 alkoxy substituted with the above C _1-6 alkoxy.
“Fluorinated C _1-6 alkoxy C _1-6 alkoxy” refers to the above C _1-6 alkoxy C _1-6 alkoxy substituted with 1 to 3 fluorine atoms.
“C _1-6 alkylthio” refers to a linear or branched alkylthio group having 1 to 6 carbon atoms, and examples thereof include methylthio and ethylthio.
“Fluorinated C _1-6 alkylthio” refers to the above C _1-6 alkylthio substituted with 1 to 3 fluorine atoms.
“Mono (di) C _1-6 alkylamino” refers to an amino mono- or di-substituted with the above C _1-6 alkyl.
“Hydroxy (mono (di) C _1-6 alkylamino)” refers to the above mono (di) C _1-6 alkylamino substituted with one or two hydroxyl groups.
“C _1-6 alkoxy C _1-6 alkylamino” refers to amino substituted with the above C _1-6 alkoxy C _1-6 alkyl.
“C _1-6 alkoxy C _1-6 alkyl (C _1-6 alkyl) amino” refers to an amino substituted with the above C _1-6 alkoxy C _1-6 alkyl and the above C _1-6 alkyl.

“C _6-10 aryl” refers to phenyl or naphthyl.
“C _6-10 aryloxy” refers to a group represented by (C _6-10 aryl) -O—, and examples thereof include phenyloxy.
“5- or 6-membered heteroaryl” refers to a 5- or 6-membered aromatic heterocyclic group containing 1 to 4 arbitrary heteroatoms selected from an oxygen atom, a sulfur atom and a nitrogen atom. , Thiazolyl, oxazolyl, isothiazolyl, isoxazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, pyrrolyl, furyl, thienyl, imidazolyl, pyrazolyl, 1,3,4-oxadiazolyl, 1,3,4-thiadiazolyl, 1,2,3-triazolyl , Tetrazolyl, furazanyl and the like.
“5- or 6-membered heteroaryloxy” refers to a group represented by (5- or 6-membered heteroaryl) -O—.
The “C _3-8 cycloalkyl” may have 1 to 2 oxo groups or 1 double bond in the ring, and may be condensed with the above C _6-10 aryl. It may be a 3- to 8-membered cycloalkyl group which may optionally be cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, 2-indanyl, 1,2,3,4-tetrahydronaphthalen-2-yl, etc. Is mentioned.
“C _3-8 cycloalkyl C _1-6 alkyl” refers to the above C _1-6 alkyl substituted with the above C _3-8 cycloalkyl.
“C _3-8 cycloalkyl C _1-6 alkoxy” refers to the above C _1-6 alkoxy substituted with the above C _3-8 cycloalkyl.
“C _3-8 cycloalkylamino” refers to a group represented by (C _3-8 cycloalkyl) -NH—, and examples thereof include cyclopropylamino, cyclobutylamino, cyclopentylamino, cyclohexylamino and the like.

“C _3-8 cycloalkoxy” refers to a group represented by (C _3-8 cycloalkyl) -O—, and includes cyclohexyloxy and the like.
“3- to 8-membered heterocycloalkyl” includes 1 to 2 heteroatoms selected from an oxygen atom, a sulfur atom and a nitrogen atom in the ring, and may have 1 to 2 oxo groups Means a 3- to 8-membered heterocycloalkyl group which may have one double bond in the ring and may be condensed with the above C _6-10 aryl, and is aziridino, azetidino, morpholino, 2 -Morpholinyl, thiomorpholino, 1-pyrrolidinyl, piperidino, 4-piperidyl, 1-piperazinyl, 2-tetrahydrofuryl, 4-tetrahydropyranyl, 1-indolinyl, 1,2,3,4-tetrahydroquinolin-1-yl, 1,2,3,4-tetrahydroisoquinolin-2-yl and the like.
“3-8 membered heterocycloalkyl C _1-6 alkyl” refers to the above C _1-6 alkyl substituted with the above 3-8 membered heterocycloalkyl.
“3-8 membered heterocycloalkyl C _1-6 alkoxy” refers to the above C _1-6 alkoxy substituted with the above 3-8 membered heterocycloalkyl.
“3 to 8 membered heterocycloalkoxy” refers to a group represented by (3 to 8 membered heterocycloalkyl) -O—.

In the phenylisonicotinic acid derivative represented by the formula (I) of the present invention, R ¹ is preferably cyano or trifluoromethyl, and more preferably cyano. R ² is preferably a hydrogen atom, a fluorine atom, C _1-6 alkyl or C _1-6 alkoxy, more preferably a hydrogen atom.

The phenylisonicotinic acid derivative represented by the formula (I) of the present invention is, for example, according to the method described in the following production methods 1 to 7 or a method equivalent thereto, the method described in other literatures or the method equivalent thereto, etc. Can be manufactured. In addition, when a protecting group is necessary, according to a conventional method (for example, the method described in Protective Groups in Organic Synthesis (4th edition)), the introduction and desorption operations can be appropriately combined. For the heating of each reaction, microwave irradiation may be used as necessary.
[Production method 1]

In the formula, L is a halogen atom, P ¹ is a protecting group for a carboxy group, P ² is a protecting group for a hydroxyl group, and R ^a is a hydrogen atom or C _1-6 alkyl (provided that two R ^a are R ^{1 to} R ³ may have the same meaning as described above, and may be bonded to each other to form a ring.

  The compound (1) and the compound (2) are subjected to a coupling reaction in an inert solvent in the presence of a base and a catalytic amount of a palladium catalyst, and then the protective group is removed to remove the formula (I) of the present invention. The phenylisonicotinic acid derivative represented by these can also be manufactured. Examples of the inert solvent include aromatic hydrocarbons such as benzene, toluene, and xylene, ethers such as diethyl ether, tetrahydrofuran, 1,4-dioxane, and 1,2-dimethoxyethane, dichloromethane, and 1,2-dichloroethane. Halogenated hydrocarbons such as chloroform, alcohols such as methanol, ethanol, 2-propanol, butanol, N, N-dimethylformamide (DMF), N-methylpyrrolidone (NMP), dimethyl sulfoxide (DMSO), water, These mixed solvents are exemplified. Examples of the base include inorganic bases such as sodium carbonate, potassium carbonate, cesium carbonate, sodium hydroxide, potassium hydroxide, sodium ethoxide, sodium methoxide, sodium hydride, potassium tert-butoxide. A base such as potassium fluoride or cesium fluoride can also be used in an aprotic solvent. Examples of the palladium catalyst include tetrakis (triphenylphosphine) palladium, dichlorobis (triphenylphosphine) palladium, 1,1'-bis (diphenylphosphino) ferrocenepalladium dichloride, and the like. The reaction temperature is usually from room temperature to reflux temperature, and the reaction time is usually from 30 minutes to 7 days, although it varies depending on the raw material used, solvent, reaction temperature and the like.

Among the phenylisonicotinic acid derivatives represented by the formula (I) of the present invention, the compound (Ia) in which R ³ is W and W is a heterocycloalkyl having a nitrogen atom at the binding site can be prepared by, for example, the production method It can also be produced by the method 2.
[Production method 2]

In the formula, ring W ¹ is heterocycloalkyl whose binding site is a nitrogen atom, L ¹ is a fluorine atom or a chlorine atom, and P ¹ , P ² , R ¹ and R ² have the same meaning as described above.

  The compound (3) and the compound (4) are subjected to a substitution reaction in an inert solvent in the presence or absence of a base and in the presence or absence of a phase transfer catalyst, and then the protective group is removed. Thus, the compound (Ia) of the present invention can be produced. Examples of the inert solvent include the aromatic hydrocarbons, ethers, halogenated hydrocarbons, alcohols, DMF, NMP, DMSO, water, mixed solvents thereof and the like described above. Examples of the base include triethylamine, diisopropylethylamine, pyridine, 2,6-lutidine, 1,8-diazabicyclo [5.4.0] undecene and other organic bases, sodium carbonate, potassium carbonate, cesium carbonate, sodium hydroxide, hydroxide Inorganic bases such as potassium, sodium ethoxide, sodium methoxide, sodium hydride, potassium tert-butoxide and the like can be mentioned. Examples of the phase transfer catalyst include tetra-n-butylammonium chloride, tetra-n-butylammonium bromide, 18-crown-6 and the like. The reaction temperature is usually from room temperature to reflux temperature, and the reaction time is usually from 30 minutes to 7 days, although it varies depending on the raw material used, solvent, reaction temperature and the like.

Among the phenylisonicotinic acid derivatives represented by the formula (I) of the present invention, the compound (Ib) in which R ³ is W and W is heterocycloalkyl or cycloalkyl in which the binding site is a carbon atom, For example, it can also be produced by the production method 3.
[Production method 3]

In the formula, ring W ² is cycloalkyl or heterocycloalkyl whose bonding site is a carbon atom, L ² is a trifluoromethanesulfonyloxy group, and P ¹ , P ² , R ¹ , R ^2, and ^Ra are the same as those described above. Has the same meaning.

  Compound (Ib) of this invention can be manufactured by removing a protecting group after performing a coupling reaction with a compound (5) and a compound (6). The coupling reaction can also be performed under the conditions described in Production Method 1.

Among the phenylisonicotinic acid derivatives represented by the formula (I) of the present invention, the compound (Ic) in which R ³ is —X—Y—Z and X is a single bond is, for example, the method of Production Method 4. Can also be manufactured.
[Production method 4]

In the formula, L ² , P ¹ , P ² , R ¹ , R ² , R ^a , Y and Z have the same meaning as described above.

  Compound (Ic) of this invention can be manufactured by removing a protecting group after performing a coupling reaction between compound (5) and compound (7). The coupling reaction can also be performed under the conditions described in Production Method 1.

Of the phenylisonicotinic acid derivatives represented by the formula (I) of the present invention, the compound (Id) in which R ³ is —X—Y—Z and X is —O— is, for example, It can also be manufactured by a method.
[Production method 5]

In the formula, L ³ is a halogen atom, and P ¹ , P ² , R ¹ , R ² , Y and Z have the same meaning as described above.

  The compound (8) can be produced by subjecting the compound (8) to a substitution reaction using the compound (9) and then removing the protecting group. The substitution reaction can also be performed under the conditions described in Production Method 2.

  Compound (3) used as a raw material for the production method can also be produced, for example, by the production method 6.

[Production method 6]

In the formula, L ¹ , L ³ , P ¹ , P ² , R ¹ , R ² and R ^a have the same meaning as described above.

  Compound (3) can be produced by subjecting compound (10) and compound (11) to a coupling reaction. The coupling reaction can also be performed under the conditions described in Production Method 1.

  Compound (5) used as a raw material for the production method can also be produced, for example, by the method of production method 7.

[Production method 7]

In the formula, L ⁴ is a halogen atom, and L ² , L ³ , P ¹ , P ² , R ¹ , R ² and R ^a have the same meaning as described above.

  Compound (13) can be produced by subjecting compound (12) to a boronation reaction. The boronation reaction is described, for example, in J. Org. Chem. Vol. 68, p. 3729 (2003), Synthesis, Vol. 18, p. 2805 (2003), Bioorg. Med. Chem. Vol. 13, p. 2859. (2005) and the like. Compound (14) can be produced by subjecting compound (13) and compound (11) to a coupling reaction. The coupling reaction can also be performed under the conditions described in Production Method 1. Compound (5) can be produced by subjecting compound (14) to a sulfonyl esterification reaction according to a conventional method.

As the protecting group used in the present invention, various protecting groups generally used in organic synthesis reactions can be used. For example, the hydroxyl protecting group includes p-methoxybenzyl group, benzyl group, methoxymethyl group, acetyl group, pivaloyl group, benzoyl group, tert-butyldimethylsilyl group, tert-butyldiphenylsilyl group, allyl group, etc. When two hydroxyl groups are adjacent to each other, an isopropylidene group, a cyclopentylidene group, a cyclohexylidene group, and the like can be given. Examples of the protecting group for the thiol group include p-methoxybenzyl group, benzyl group, acetyl group, pivaloyl group, benzoyl group, and benzyloxycarbonyl group. Examples of the amino-protecting group include benzyloxycarbonyl group, tert-butoxycarbonyl group, benzyl group, p-methoxybenzyl group, trifluoroacetyl group, acetyl group, phthaloyl group and the like. Examples of the protecting group for the carboxy group include a C _1-6 alkyl group, a benzyl group, a tert-butyldimethylsilyl group, and an allyl group.

  The compound represented by the formula (I) of the present invention can be isolated and purified by a conventional separation means such as a fractional recrystallization method, a purification method using chromatography, a solvent extraction method, a solid phase extraction method and the like. .

  The phenylisonicotinic acid derivative represented by the formula (I) of the present invention can be converted into a pharmacologically acceptable salt thereof by a conventional method. Such salts include acid addition salts with mineral acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, nitric acid, phosphoric acid, formic acid, acetic acid, methanesulfonic acid, benzenesulfonic acid, p-toluene. Acid addition with organic acids such as sulfonic acid, propionic acid, citric acid, succinic acid, tartaric acid, fumaric acid, butyric acid, oxalic acid, malonic acid, maleic acid, lactic acid, malic acid, carbonic acid, benzoic acid, glutamic acid, aspartic acid Salt, sodium salt, potassium salt, calcium salt, magnesium salt, zinc salt, salt with inorganic base such as lithium salt, aluminum salt, N-methyl-D-glucamine, N, N′-dibenzylethylenediamine, 2-amino Ethanol, tris (hydroxymethyl) aminomethane, arginine, lysine, piperazine, choline, diethylamine, 4-phenyl-cyclohexane Addition salts with organic bases may be mentioned.

  Among the phenylisonicotinic acid derivatives represented by the formula (I) of the present invention, the compound having an unsaturated bond includes two geometric isomers, a cis (Z) isomer and a trans (E) isomer. Although a compound exists, in the present invention, any of these compounds may be used, or a mixture thereof may be used.

  Among the phenylisonicotinic acid derivatives represented by the formula (I) of the present invention, a compound having an asymmetric carbon atom includes a compound having an R configuration and a compound having an S configuration for each asymmetric carbon, In the present invention, any optical isomer may be used, and a mixture of these optical isomers may be used.

  The phenylisonicotinic acid derivative represented by the formula (I) of the present invention may have various tautomers, and the compound of the present invention also includes those tautomers.

In the present invention, a prodrug refers to a compound that is converted into a compound represented by formula (I) in vivo. The prodrug of the compound represented by the formula (I) of the present invention is a hydroxyl group, an amino group in the compound represented by the formula (I) by a conventional method using a prodrug reagent such as a corresponding halide. A group constituting a prodrug is appropriately introduced into one or more arbitrary groups selected from a carboxy group and other groups capable of forming a prodrug according to a conventional method, and then isolated and purified according to a conventional method as needed. ("Monthly Pharmaceutical Affairs Clinical Pharmacokinetics for Proper Use of Drugs," March 2000 Extra Issue, Volume 42, Volume 4, p.669-707, "New Drug Delivery System "See CMC Co., Ltd., January 31, 2000, p. 67-173). Examples of the group constituting the prodrug used for the hydroxyl group include C _1-6 alkyl-CO— such as acetyl, propionyl, butyryl, isobutyryl, pivaloyl; C _6-10 aryl-CO— such as benzoyl; C _{1 -6} alkyl-O-C _1-6 alkylene-CO-; C _1-6 alkyl-OCO-C _1-6 alkylene-CO-; methyloxycarbonyl, ethyloxycarbonyl, propyloxycarbonyl, isopropyloxycarbonyl, tert- C _1-6 alkyl-OCO— such as butyloxycarbonyl; C _1-6 alkyl-O—C _1-6 alkylene-OCO—; acetyloxymethyl, pivaloyloxymethyl, 1- (acetyloxy) ethyl, 1 - (pivaloyloxy) _{C 1-6} alkyl -COO-C ethyl, etc. _-6 alkylene; methoxycarbonyloxymethyl, 1- (methoxycarbonyloxy) ethyl, ethoxycarbonyloxymethyl, 1- (ethoxycarbonyloxy) ethyl, isopropyloxycarbonyloxy methyl, 1- (isopropyloxycarbonyloxy) ethyl, tert- C _1-6 alkyl-OCOO-C _1-6 alkylene such as butyloxycarbonyloxymethyl and 1- (tert-butyloxycarbonyloxy) ethyl; C such as cyclohexyloxycarbonyloxymethyl and 1- (cyclohexyloxycarbonyl) ethyl _3-8 cycloalkyl-OCOO-C _1-6 alkylene; esters and amides with amino acids such as glycine;
Examples of the group constituting the prodrug used in the carboxy group include C _1-6 alkyl such as methyl, ethyl, propyl, isopropyl, butyl, tert-butyl; pivaloyloxymethyl, acetyloxymethyl, 1- C _1-6 alkyl-COO-C _1-6 alkylene such as (pivaloyloxy) ethyl, 1- (acetyloxy) ethyl; ethyloxycarbonyloxymethyl, 1- (ethyloxycarbonyloxy) ethyl, isopropyloxycarbonyloxymethyl, 1- (isopropyloxycarbonyloxy) ethyl, tert- butyloxycarbonyl oxymethyl, 1- (tert-butyloxycarbonyl oxy) _{C 1-6} alkyl _{-OCOO-C 1-6} alkylene-ethyl and the like; cyclohexyloxy-carbonitrile Rumechiru, 1 can be mentioned _{C 3-8} cycloalkyl _{-OCOO-C 1-6} alkylene group such as (cyclohexyloxycarbonyl) ethyl and the like.

  In the present invention, pharmacologically acceptable salts also include solvates with pharmacologically acceptable solvents such as water or ethanol.

  The pharmaceutical composition of the present invention is useful for the prevention or treatment of diseases involving high blood uric acid levels such as hyperuricemia, gout nodules, gout arthritis, renal disorders due to hyperuricemia, urolithiasis, etc. In particular, it is useful for hyperuricemia.

  When the pharmaceutical composition of the present invention is used for actual prevention or treatment, the dose of the compound represented by formula (I) or a prodrug thereof, or a pharmacologically acceptable salt thereof, which is an active ingredient thereof, It is determined as appropriate depending on the patient's age, sex, weight, disease, degree of treatment, etc. For example, in the case of oral administration, it may be administered once or divided into several doses in the range of about 1 to 2000 mg per day for an adult. it can.

  When the pharmaceutical composition of the present invention is used for actual prevention or treatment, various dosage forms are used orally or parenterally depending on the usage. For example, powders, fine granules, granules, Oral preparations such as tablets, capsules and dry syrups are preferred.

  These pharmaceutical compositions are prepared according to a conventional method by appropriately mixing pharmaceutical additives such as excipients, disintegrants, binders, lubricants and the like according to the dosage form in accordance with ordinary pharmacological methods. Can be manufactured.

  For example, a powder is added to an active ingredient as needed, and an appropriate excipient | filler, a lubricant, etc. are added and mixed well to make a powder. For example, tablets are added to the active ingredients with appropriate excipients, disintegrants, binders, lubricants, etc., and compressed into tablets according to conventional methods. Tablets, sugar-coated tablets, enteric-coated skin tablets, etc. For example, a capsule is prepared by adding an appropriate excipient, lubricant, etc. to an active ingredient and mixing well, or after granulating or finely granulating it according to a conventional method, filling it into an appropriate capsule and To do. Furthermore, in the case of such an orally administered preparation, an immediate release or sustained release preparation can be prepared depending on the prevention or treatment method.

  In addition to the compound represented by the formula (I) of the present invention or a prodrug thereof, or a pharmacologically acceptable salt thereof, other hyperuricemia or gout may be used in combination. it can. Examples of therapeutic agents for hyperuricemia that can be used in the present invention include urinary alkalizing agents such as sodium hydrogen carbonate, potassium citrate, and sodium citrate. Examples of the gout treatment include colchicine, non-steroidal anti-inflammatory drugs such as indomethacin, naproxen, fenbufen, pranoprofen, oxaprozin, ketoprofen, etoroxixib, tenoxicam, and steroids. In the present invention, in addition to the active ingredient of the present invention, it can be used in combination with at least one of these drugs, but the pharmaceutical composition combined with at least one of these drugs is effective for the present invention. It is not limited to a single pharmaceutical composition formulated at the same time as the ingredients, but also includes administration forms that are used simultaneously or at different intervals as a pharmaceutical composition produced separately from the pharmaceutical composition containing the active ingredient of the present invention. In addition, when used in combination with a drug other than the active ingredient of the present invention, the dose of the compound of the present invention can be reduced according to the dose of the other drug used in combination. It is possible to obtain an advantageous effect that is more than an additive effect in terms of prevention or treatment, and to avoid or reduce the side effects of other drugs used in combination.

  The phenylisonicotinic acid derivative represented by the formula (I) of the present invention expresses excellent xanthine oxidase and URAT1 inhibitory activity, suppresses uric acid production, and promotes uric acid excretion. Therefore, the phenylisonicotinic acid derivative represented by the formula (I) of the present invention or a prodrug thereof or a pharmacologically acceptable salt thereof can remarkably suppress an increase in serum uric acid level, and hyperuricemia It is useful as a preventive or therapeutic agent for diseases caused by abnormal serum uric acid levels such as infectious diseases.

  The content of the present invention will be described in more detail with reference examples, examples and test examples below, but the present invention is not limited to the content.

Reference example 1
Ethyl 2-bromo-5-methoxymethoxyisonicotinate Diisopropylamine (9.49 g) in tetrahydrofuran (210 mL) was charged with 2.6 M n-butyllithium hexane solution (35.7 mL) at −78 ° C. in an argon atmosphere. Dropped and stirred for 15 minutes. A solution of 2-bromo-5-fluoropyridine (15.0 g) in tetrahydrofuran (210 mL) was added dropwise and stirred at the same temperature for 2 hours. An excessive amount of dry ice was added to the reaction solution, stirred at room temperature for 1 hour, 1 mol / L hydrochloric acid and water were added, and the mixture was extracted with diethyl ether. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was washed with diethyl ether to obtain 2-bromo-5-fluoroisonicotinic acid (15.9 g).
This compound (15.9 g) was dissolved in 1,3-dimethyl-2-imidazolidinone (144 mL), sodium hydroxide (11.5 g) was added, and the mixture was stirred at 130 ° C. for 3 hr. After allowing to cool, 2 mol / L hydrochloric acid (144 mL) was added to the reaction solution, and the precipitated solid was collected by filtration and washed with water. This was dried at 50 ° C. under reduced pressure for 12 hours to obtain 2-bromo-5-hydroxyisonicotinic acid (15.4 g).
This compound (15.4 g) was dissolved in ethanol (140 mL), thionyl chloride (33.5 g) was added dropwise at 0 ° C. over 15 minutes, and the mixture was heated to reflux for 24 hours. After allowing to cool, the reaction solution was concentrated under reduced pressure to obtain ethyl 2-bromo-5-hydroxyisonicotinate (17.3 g).
This compound (17.3 g) was dissolved in dichloromethane (70.0 mL), N, N-diisopropylethylamine (22.7 g) was added, and then chloromethyl methyl ether (11.3 g) was added at 0 ° C. for 10 minutes. The solution was added dropwise and stirred at room temperature for 24 hours. To the reaction solution was added 1 mol / L hydrochloric acid and water, and the mixture was extracted with diethyl ether. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain the title compound (14.9 g).

Reference example 2
2- (3-Cyano-4-fluorophenyl) -5-methoxymethoxyisonicotinic acid ethyl 2-bromo-5-methoxymethoxyisonicotinate (1.32 g), 3-cyano-4-fluorophenylboronic acid ( To a solution of 0.68 g) and cesium fluoride (0.75 g) in 1,2-dimethoxyethane (10 mL) was added tetrakis (triphenylphosphine) palladium (0) (0.48 g) under an argon atmosphere. Stir at 7 ° C. for 7 hours. After allowing to cool, water was added to the reaction solution, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain the title compound (0.45 g).

Reference example 3
Ethyl 2- [3-cyano-4- (pyrrolidin-1-yl) phenyl] -5-methoxymethoxyisonicotinate 2- (3-cyano-4-fluorophenyl) -5-methoxymethoxyisonicotinate (0 0.08 g) in dimethylsulfoxide (1 mL) was added pyrrolidine (0.09 g), and the mixture was stirred at 55 ° C. for 15 hours. After allowing to cool, 1 mol / L hydrochloric acid and water were added to the reaction solution, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain the title compound (0.06 g).

Reference example 4
Ethyl 5-benzyloxy-2- (3-cyano-4-morpholinophenyl) isonicotinate To a solution of 2-bromo-5-fluoropyridine (5.00 g) in tetrahydrofuran (100 mL) at −78 ° C. under an argon atmosphere. A 2.6M n-butyllithium hexane solution (12.0 mL) was added dropwise, and the mixture was stirred for 3 hours, and then an excessive amount of dry ice was added to the reaction solution. After stirring at room temperature for 12 hours, water (100 mL) was added, washed with diethyl ether, 1 mol / L hydrochloric acid and water were added to the aqueous phase, and the mixture was concentrated under reduced pressure. The obtained residue was dissolved in dimethylformamide (5 mL), iodoethane (0.71 g) and potassium carbonate (0.63 g) were added, and the mixture was stirred at room temperature for 15 hours. Water was added to the reaction solution, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain ethyl 2-bromo-5-fluoroisonicotinate (2.94 g).
Benzyl alcohol (1.54 g) was added to a solution of sodium hydride (mineral oil 40% added, 0.34 g) in tetrahydrofuran (30 mL), stirred at room temperature for 5 minutes, and then ethyl 2-bromo-5-fluoroisonicotinate. A solution of (2.94 g) in tetrahydrofuran (30 mL) was added and stirred for 2 hours. Water was added to the reaction solution, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain ethyl 5-benzyloxy-2-bromoisonicotinate (2.40 g).
This compound (0.30 g) was dissolved in 1,2-dimethoxyethane (4 mL) and 3-cyano-4-fluorophenylboronic acid (0.13 g) and cesium fluoride (0.15 g) were added. Tetrakis (triphenylphosphine) palladium (0) (0.09 g) was added under an argon atmosphere, and the mixture was stirred at 70 ° C. for 8 hours. After allowing to cool, water was added to the reaction solution, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain ethyl 5-benzyloxy-2-bromoisonicotinate (0.12 g).
This compound (0.08 g) was dissolved in dimethyl sulfoxide (1 mL), morpholine (0.11 g) was added, and the mixture was stirred at 55 ° C. for 15 hours. After allowing to cool, water was added to the reaction solution, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain the title compound (0.05 g).

Reference Example 5
Ethyl 2- (4-benzyloxy-3-cyanophenyl) -5-methoxymethoxyisonicotinate 2-Benzyloxy-5-bromobenzonitrile (4.70 g), bis (pinacolato) diboron (4.35 g) and acetic acid To a solution of potassium (4.80 g) in dimethylformamide (80 mL) was added palladium (II) acetate (0.11 g) under an argon atmosphere, and the mixture was stirred at 80 ° C. for 5 hours. After allowing to cool, water was added to the reaction solution, the insoluble material was removed through celite, and the mixture was extracted with diethyl ether. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was dissolved in dimethylformamide (32 mL), and in an argon atmosphere, ethyl 2-bromo-5-methoxymethoxyisonicotinate (4.97 g), cesium carbonate (7.97 g), tetrakis (triphenylphosphine) Palladium (0) (0.94 g) and water (12 mL) were added, and the mixture was stirred at 80 ° C. for 3 hours. After allowing to cool, water and dichloromethane were added to the reaction solution, insoluble matters were removed by passing through Celite, and the mixture was extracted with dichloromethane. The organic layer was separated and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain the title compound (4.33 g).

Reference Example 6
Ethyl 2- (3-cyano-4-hydroxyphenyl) -5-methoxymethoxyisonicotinate Ethyl 2- (4-benzyloxy-3-cyanophenyl) -5-methoxymethoxyisonicotinate (4.33 g) in methanol To a mixed solution of (50 mL) and ethyl acetate (50 mL), palladium carbon (0.77 g) was added, and the mixture was stirred at room temperature for 5 hours in a hydrogen atmosphere. After making the atmosphere of argon, insolubles were removed by passing through Celite and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain the title compound (1.75 g).

Reference Example 7
Ethyl 2- (3-cyano-4-propoxyphenyl) -5-methoxymethoxyisonicotinate Dimethylformamide of ethyl 2- (3-cyano-4-hydroxyphenyl) -5-methoxymethoxyisonicotinate (0.07 g) To the (1 mL) solution, 1-iodopropane (0.07 g) and potassium carbonate (0.07 g) were added and stirred at 80 ° C. for 18 hours. After allowing to cool, water was added to the reaction solution, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain the title compound (0.05 g).

Reference Example 8
Ethyl 2- (3-cyano-4-trifluoromethanesulfonyloxyphenyl) -5-methoxymethoxyisonicotinate 2- (3-cyano-4-hydroxyphenyl) -5-methoxymethoxyisonicotinate (0.16 g) After adding pyridine (0.12 g) to a dichloromethane (1 mL) solution, trifluoromethanesulfonic anhydride (0.16 g) was added dropwise under ice cooling, and the mixture was stirred for 1 hour. To the reaction solution was added 1 mol / L hydrochloric acid and water, dichloromethane was added to separate the organic layer, and the mixture was concentrated under reduced pressure to obtain the title compound (0.23 g).

Reference Example 9
Ethyl 2- (4-butyl-3-cyanophenyl) -5-methoxymethoxyisonicotinate Ethyl 2- (3-cyano-4-trifluoromethanesulfonyloxyphenyl) -5-methoxymethoxyisonicotinate (0.05 g) Butylboronic acid (0.01 g), tetrakis (triphenylphosphine) palladium (0.01 g) and potassium carbonate (0.21 g) were added to a toluene (0.75 mL) solution under an argon atmosphere at 80 ° C. for 3 hours. After stirring, the reaction solution was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain the title compound (0.02 g).

Reference Example 10
Ethyl 2- (3-cyano-4-phenethylphenyl) -5-methoxymethoxyisonicotinate 2- (3-cyano-4-trifluoromethanesulfonyloxyphenyl) -5-methoxymethoxyisonicotinate (0.21 g) To a toluene (1.20 mL) solution was added trans-2-phenylvinylboronic acid (0.07 g), tetrakis (triphenylphosphine) palladium (0.08 g) and potassium carbonate (0.09 g) under an argon atmosphere. After stirring at 80 ° C. for 3 hours, the reaction solution was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to give 2- [3-cyano-4-((E) styryl) phenyl] -5. -Ethyl methoxymethoxyisonicotinate (0.12 g) was obtained.
This compound (0.08 g) was dissolved in methanol (1.8 mL) and ethyl acetate (1.8 mL), palladium carbon (0.01 g) was added, and the mixture was stirred at room temperature for 3 hr in a hydrogen atmosphere. After making the atmosphere of argon, insolubles were removed by passing through Celite and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution) to obtain the title compound (0.08 g).

Reference Example 11
2- (4-Benzyloxy-3-cyano-5-methylphenyl) -5-methoxymethoxyisonicotinate 2-benzyloxy-5-bromo-3-methylbenzonitrile (1.66 g), bis (pinacolato) To a solution of diboron (1.54 g) and potassium acetate (1.62 g) in dimethylsulfoxide (40 mL) was added 1,1′-bis (diphenylphosphino) ferrocenepalladium (II) dichloride-dichloromethane (0.13 g) under an argon atmosphere. ) And stirred at 80 ° C. for 1 hour. After allowing to cool, water and diethyl ether were added to the reaction solution, and insoluble matters were removed by passing through Celite, followed by extraction with diethyl ether. Subsequently, the organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was dissolved in dimethylformamide (11 mL), and ethyl 2-bromo-5-methoxymethoxyisonicotinate (1.60 g), cesium carbonate (2.69 g), tetrakis (triphenylphosphine) under an argon atmosphere. Palladium (0) (0.32 g) and water (2.2 mL) were added, and the mixture was stirred at 80 ° C. for 2 hours. After allowing to cool, water and ethyl acetate were added to the reaction solution, and insoluble matters were removed by passing through Celite, followed by extraction with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane) to obtain the title compound (1.67 g).

Reference Examples 12-16
The compound of Reference Example 12 was prepared in the same manner as in Reference Example 4, the compound of Reference Examples 13-14 was prepared in the same manner as in Reference Example 5, the compound of Reference Example 15 was prepared in the same manner as in Reference Example 7, and the reference example 9 was used. The compound of Reference Example 16 was synthesized using the corresponding starting materials by the same method.

Example 1
2- (3-Cyano-4-morpholinophenyl) -5-hydroxyisonicotinic acid Ethyl 5-benzyloxy-2- (3-cyano-4-morpholinophenyl) isonicotinate (0.047 g) in methanol (2 mL) Ammonium formate (0.003 g) and palladium carbon (0.010 g) were added to a mixed solution of tetrahydrofuran and tetrahydrofuran (2 mL), and the mixture was stirred at room temperature for 2 hours in a hydrogen atmosphere. After making the atmosphere of argon, insolubles were removed by passing through Celite and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution solvent: ethyl acetate / hexane solution = 0.05 to 0.50) to give 2- (3-cyano-4-morpholinophenyl) -5-hydroxyisonicotinic acid. Ethyl (0.025 g) was obtained.
This compound (0.025 g) was dissolved in tetrahydrofuran (3.0 mL), ethanol (1.5 mL) and water (1.5 mL), lithium hydroxide monohydrate (0.04 g) was added, and the mixture was stirred at 35 ° C. Stir for 24 hours. After adding 1 mol / L hydrochloric acid and water to the reaction solution, the precipitated solid was collected by filtration and washed with water. This was dried under reduced pressure at 50 ° C. for 12 hours to obtain the title compound (0.016 g).

Example 2
2- (4-Butyl-3-cyanophenyl) -5-hydroxyisonicotinic acid Ethyl 2- (4-butyl-3-cyanophenyl) -5-methoxymethoxyisonicotinate (0.02 g) in tetrahydrofuran (1. 0 mL), ethanol (0.5 mL) and water (0.5 mL) mixed solution was added lithium hydroxide monohydrate (0.01 g), and the mixture was stirred at room temperature for 3 hours, and then 2 mol / L hydrochloric acid (0. 48 mL) was added, and the mixture was stirred at 50 ° C. for 2 hours. After allowing to cool, water was added to the reaction solution, and the precipitated solid was collected by filtration and washed with water. This was dried under reduced pressure at 50 ° C. for 12 hours to obtain the title compound (0.01 g).

Examples 3-14
Using the corresponding raw materials, the compounds of Examples 3, 6 and 8 were prepared in the same manner as in Example 1, and the compounds of Examples 4, 5, 7 and 9-14 were prepared in the same manner as in Example 2. Synthesized.

Examples 15-86
In the same manner as in Example 2, the compounds of Examples 15 to 86 were synthesized using the corresponding raw materials.

Example 87
The compound of Example 87 was synthesized in the same manner as in Example 1 using the corresponding starting materials.

The chemical structural formulas and ¹ H-NMR data of Reference Example Compounds 1-16 and Example Compounds 1-87 are shown in Tables 1-11.

The abbreviations in the table are Ref No. Is a reference example number, Ex No. is an example number, Strc is a chemical structural formula, Solv is a ¹ H-NMR measurement solvent, Bn is a benzyl group, Me is a methyl group, Et is an ethyl group, MOM is a methoxymethyl group, Tf Represents a trifluoromethanesulfonyl group, respectively.

Test example 1
Xanthine oxidase inhibitory activity (1) Preparation of test compound The test compound was dissolved in DMSO (manufactured by Wako Pure Chemical Industries) to a concentration of 40 mM, and then diluted with phosphate buffered saline (PBS). Prepared to the desired concentration.

(2) Measuring method Xanthine oxidase (derived from bovine milk, manufactured by Sigma) was adjusted to 0.02 units / mL with phosphate buffered saline (PBS), and 50 μL / well was added to a 96-well plate. Further, 50 μL / well of a test compound diluted with PBS was added. 200 μM xanthine (manufactured by Wako Pure Chemical Industries, Ltd.) prepared using PBS was added at 100 μL / well and allowed to react at room temperature for 10 minutes. Absorbance was measured under the condition of 290 nm using a microplate reader Spectramax Plus 384 (manufactured by Molecular Devices). The concentration (IC ₅₀ ) of the test compound that inhibits 50% was calculated with the absorbance under the condition where xanthine was not added as 0% and the control without the test compound as 100% (Table 12). In the table, Ex. No shows an Example number.

Test example 2
Uric acid transport inhibitory activity using human URAT1-expressing cells
(1) Preparation of human URAT1 transient expression cells Human URAT1 full-length cDNA (NCBI Accession No. NM — 144585) was subcloned into the expression vector pcDNA3.1 (manufactured by Invitrogen). Human URAT1 expression vector was introduced into COS7 cells (RIKEN CELL BANK RCB0539) using Lipofectamine 2000 (Invitrogen). COS7 cells were seeded in a collagen-coated 24-well plate (Nippon Becton Dickinson) to 90-95% confluent, and contained 10% fetal bovine serum (Sanko Junyaku Co.) D-MEM medium (Invitrogen) Was cultured for 2 hours under conditions of 37 ° C. and 5% CO ₂ . 2 μL of Lipofectamine 2000 per well was diluted with 50 μL of OPTI-MEM (manufactured by Invitrogen) and allowed to stand at room temperature for 7 minutes (hereinafter referred to as Lipo2000-OPTI). 0.8 μg human URAT1 expression vector per well is diluted with 50 μL of OPTI-MEM (manufactured by Invitrogen), mixed gently with Lipo2000-OPTI, allowed to stand at room temperature for 25 minutes, and then 100 μL per well. Added to COS7 cells. Furthermore, COS7 cells were cultured for 2 days under conditions of 37 ° C. and 5% CO ₂ and subjected to measurement of uptake inhibition activity.

(2) Preparation of test compound The test compound was dissolved in DMSO (manufactured by Wako Pure Chemical Industries, Ltd.) to a concentration of 10 mM, and then a pretreatment buffer (125 mM sodium gluconate, 4.8 mM potassium gluconate, 1 .2 mM potassium dihydrogen phosphate, 1.2 mM magnesium sulfate, 1.3 mM calcium gluconate, 5.6 mM glucose, 25 mM hepes, pH 7.4) did. A pretreatment buffer containing no test compound was used as a control. Further, an equal amount of pretreatment buffer containing ¹⁴ C-labeled uric acid (American Radiolabeled Chemicals, Inc.) was added to the test compound and the control to finally produce an assay buffer containing 20 μM uric acid. .

(3) Measurement method All tests were performed on a hot plate at 37 ° C. Pretreatment buffer and assay buffer were used after warming at 37 ° C. The medium was removed from the plate and 700 μL of pretreatment buffer was added and preincubated for 10 minutes. After repeating the same operation, the pretreatment buffer was removed, assay buffer was added at 400 μL / well, and an uptake reaction was performed for 5 minutes. Immediately after the reaction was completed, the assay buffer was removed, and ice-cooled pretreatment buffer was added at a rate of 1.2 mL / well, and the cells were washed twice. 0.2N sodium hydroxide was added at 300 μL / well to lyse the cells. Transfer the cell lysate to a picoplate (Perkin Elmer), add Microcinti 40 (Perkin Elmer) at 600 μL / well, mix, and then use a liquid scintillation counter (Perkin Elmer) to perform radioactivity. It was measured. Further, COS7 cells into which no URAT1 expression vector was introduced were also measured for radioactivity under the same conditions as in the control. The inhibition rate at 10 μM of the test compound was calculated by the following formula (Table 13).

阻害率（％）＝［１−（Ｂ−Ｃ）／（Ａ−Ｃ）］Ｘ １００
Ａ：対照の放射活性
Ｂ：試験化合物を加えた場合の放射活性
Ｃ：ＵＲＡＴ１発現ベクターを導入していないＣＯＳ７細胞の放射活性

Inhibition rate (%) = [1- (BC) / (AC)] X 100
A: Radioactivity of control B: Radioactivity when test compound is added C: Radioactivity of COS7 cells not introduced with URAT1 expression vector

  Since the phenylisonicotinic acid derivative represented by the formula (I) of the present invention or a prodrug thereof, or a pharmacologically acceptable salt thereof has an excellent xanthine oxidase and URAT1 inhibitory action, It exhibits uric acid excretion promoting action and can reduce blood uric acid level. Therefore, the present invention can provide a preventive or therapeutic agent for hyperuricemia, gouty nodule, gout arthritis, renal disorder due to hyperuricemia, urolithiasis, and the like.

Claims (21)

Formula (I)

[Where,
R ¹ is a cyano, trifluoromethyl or chlorine atom;
R ² represents a hydrogen atom, a fluorine atom, a chlorine atom, a hydroxyl group, amino, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, fluorine C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy, fluorinated C _1-6 Alkoxy C _1-6 alkoxy, C _1-6 alkylthio, fluorinated C _1-6 alkylthio, C _2-6 alkenyl, mono (di) C _1-6 alkylamino, hydroxy (mono (di) C _1-6 alkylamino _{), C 1-6} alkoxy _{C 1-6 alkylamino, C 1-6} alkoxy _{C 1-6} alkyl _{(C 1-6} alkyl) _{amino, C 3-8} cycloalkyl , 3-8 membered _{heterocycloalkyl, C 3-8} cycloalkyl _{C 1-6} alkyl, 3-8 membered heterocycloalkyl _{C 1-6 alkyl, C 3-8} cycloalkyl _{C 1-6} alkoxy, 3 8-membered heterocycloalkyl C _1-6 alkoxy, C _3-8 cycloalkoxy or 3-8 membered heterocycloalkoxy (wherein the C _3-8 cycloalkyl, 3-8 membered heterocycloalkyl, C _{3 -8} cycloalkoxy and 3-8 membered heterocycloalkoxy, and C _3-8 cycloalkyl C _1-6 alkyl, 3-8 membered heterocycloalkyl C _1-6 alkyl, C _3-8 cycloalkyl C _{1- 6} alkoxy and 3-8 membered heterocycloalkyl _{C 1-6} 3-8 membered heterocycloalkyl and _{C 3-8} Shikuroaruko in alkoxy Shi moiety may have 1 to 3 identical or different groups selected from α each substituent group);
Substituent group α is halogen atom, hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy, fluorinated C _1-6 alkoxy C _1-6 alkoxy ;
R ³ represents a group represented by W or —X—Y—Z;
W is octahydrocyclopenta [c] pyrrolyl, octahydroisoindolyl, 2-oxa-5-azabicyclo [2.2.1] hept-5-yl, C _3-8 cycloalkylamino, or halogen, respectively Atom, hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C Selected from the group consisting of _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy C _1-6 alkoxy identical or different radicals from 1 to 3 have good _{C 3-8} also be cycloalkyl, 3-8 membered _{heterocycloalkyl, C 3-8} cycloalk Alkoxy, 3-8 membered heterocycloalkyl _{alkoxy, C 6-10} aryloxy, or 5 or 6-membered heteroaryloxy;
X is a single bond, —N (R ⁵ ) —, —O— or —S— (R ⁵ is a hydrogen atom or C _1-6 alkyl);
Y is C _1-6 alkylene or C _2-6 alkenylene;
Z is hydrogen atom; hydroxyl group; C _1-6 alkoxy; fluorinated C _1-6 alkoxy; thiol; C _1-6 alkylthio; fluorinated C _1-6 alkylthio; or halogen atom, hydroxyl group, C _1-6 alkyl, respectively. C _3-8 cycloalkyl optionally having 1 to 3 identical or different groups selected from the group consisting of fluorinated C _1-6 alkyl, C _1-6 alkoxy and fluorinated C _1-6 alkoxy , 3-8 membered heterocycloalkyl, C _6-10 aryl or 5 or 6 membered heteroaryl (wherein C _6-10 aryl is each substituted with C _1-6 alkoxy on two adjacent atoms in the ring, when having the, -OCH ₂ O these together _{-, - O (CH 2)} 2 O -, - OCF 2 O- or -O _{(CF 2)} may form a 2 O-); Phenyl isonicotinic acid derivative thereof represented by certain] or salt thereof pharmacologically acceptable. R ³ is a group represented by W ⁰ or —X—Y—Z (W ⁰ is a halogen atom, a hydroxyl group, C _1-6 alkyl, a fluorinated C _1-6 alkyl, or a hydroxy C _1-6 alkyl, respectively. , C _1-6 alkoxy C _1-6 alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 C _3-8 cycloalkyl optionally having 1 to 3 identical or different groups selected from the group consisting of alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy C _1-6 alkoxy; 8-membered _{heterocycloalkyl, C 3-8} cycloalkoxy, a 3-8 membered heterocycloalkyl _{alkoxy, C 6-10} aryloxy, or 5 or 6 membered heteroaryloxy, X, Y Fine Z has the same meaning and is) and the claim 1 phenyl isonicotinate inducing member or a pharmacologically acceptable salt thereof. Salts phenyl isonicotinic acid derivative of claim 1 or 2 wherein R ¹ is cyano or allowed its pharmacologically. Formula (Ia)

[Wherein, W ¹ is a nitrogen atom at the bonding site, a halogen atom, a hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _{1- 6} alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy is a 3- to 8-membered heterocycloalkyl optionally having 1 to 3 identical or different groups selected from the group consisting of C _1-6 alkoxy. salts phenyl isonicotinic acid induced body or allowed its pharmacologically according to any one of to 3. W ¹ is a halogen atom, a hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, or fluorinated C _1-6 alkoxy, respectively. C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy C _1-6 alkoxy identical or different radicals selected from the group consisting of which may have 1-3, pyrrolidine, piperidine, azepane or morpholine, phenyl isonicotinic acid derivative of claim 4, wherein or a pharmaceutically Acceptable salt. W ¹ represents the following formula (IIa)

[Wherein, R ^3a represents a hydrogen atom, a halogen atom, a hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy or fluorinated C _1-6 alkoxy C _1-6 alkoxy;
R ^3b is a hydrogen atom or a fluorine atom; a piperidine represented by a is], claim 4 phenyl isonicotinate inducing member or a pharmacologically acceptable salt thereof. R ^3a and R ^3b are both a fluorine atom, according to claim 6 biaryl salt isonicotinic acid induced body or is a pharmaceutically acceptable description. Salts W ¹ is a morpholine or 1,4-oxazepan not have a substituent, phenyl isonicotinic acid derivative of claim 4, wherein or is a pharmaceutically acceptable. Formula (Ib)

[Wherein, W ² is a carbon atom at the bonding site, and is a halogen atom, hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _{1, respectively. -6} alkyl, fluorinated C _1-6 alkoxy C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorine C _1-6 alkoxy is C _3-8 cycloalkyl or 3-8 membered heterocycloalkyl optionally having 1 to 3 identical or different groups selected from the group consisting of C _1-6 alkoxy phenyl isonicotinic acid induced body or a pharmacologically acceptable salt thereof according to claim 1, represented by]. W ² is a halogen atom, a hydroxyl group, C _1-6 alkyl, fluorinated C _1-6 alkyl, hydroxy C _1-6 alkyl, C _1-6 alkoxy C _1-6 alkyl, or fluorinated C _1-6 alkoxy, respectively. C _1-6 alkyl, C _1-6 alkoxy, fluorinated C _1-6 alkoxy, hydroxy C _1-6 alkoxy, C _1-6 alkoxy C _1-6 alkoxy and fluorinated C _1-6 alkoxy C _1-6 alkoxy identical or different radicals selected from the group consisting of which may have 1 to 3, cyclopropyl, cyclopentyl, cyclohexyl or tetrahydropyranyl cycloheteroalkyl, phenyl isonicotinic acid derivative of claim 9, wherein also Is a pharmacologically acceptable salt thereof. Salts W ² is a cyclopropyl which does not have a substituent, phenyl isonicotinic acid derivative of claim 9, wherein or is a pharmaceutically acceptable. X is a single bond; which Y is C _1-6 alkylene, salt phenyl isonicotinate induction body or allowed its pharmacologically according to any one of claims 1 to 3. X is -N (R 5) ^- or -O- (R ⁵ is a hydrogen atom or a methyl group), phenyl isonicotinic acid derivative as claimed in claim 1 or its pharmacologically Acceptable salt. Y is C _1-6 alkylene, salt phenyl isonicotinic acid derivative of claim 13, wherein or is a pharmaceutically acceptable. Z is a hydrogen atom, salts phenyl isonicotinate induction body or allowed its pharmacologically according to any one of claims 12 to 14. Salts phenyl isonicotinic acid induced body or allowed its pharmacologically according to any of claims 1 to 15 R ² is a hydrogen atom. Phenylisonicotinic acid derivative selected from the following group or a pharmacologically acceptable salt thereof:

URAT1 an inhibitor, salt phenyl isonicotinate induction body or allowed its pharmacologically according to any one of claims 1 to 17. A pharmaceutical composition comprising the phenylisonicotinic acid derivative according to any one of claims 1 to 17 or a pharmacologically acceptable salt thereof as an active ingredient. 20. The pharmaceutical composition according to claim 19 , which is used for prevention or treatment of a disease selected from hyperuricemia, gouty nodule, gout arthritis, renal disorder due to hyperuricemia and urolithiasis. The pharmaceutical composition according to claim 20 , which is used for prevention or treatment of hyperuricemia.