JP5522716B2 - Microparticles, UV-proof base, microparticle manufacturing method, and UV-proof base manufacturing method - Google Patents

Description

  The present invention relates to microparticles, an ultraviolet-proof base, a method for producing microparticles, and a method for producing an ultraviolet-proof base that are used to prevent the transmission of ultraviolet rays.

  As is well known, carcinogenesis due to an increase in solar ultraviolet radiation has become a major problem. Therefore, in recent years, it is necessary to protect the skin from ultraviolet rays regardless of gender. Therefore, in order to protect the skin from ultraviolet rays, development of cosmetics having a function of preventing the transmission of ultraviolet rays (hereinafter also referred to as an ultraviolet protection function or an ultraviolet protection function), that is, UV-cut cosmetics, has been underway. In particular, so-called nanocosmetics that contain nanoparticles or microparticles as components are attracting attention. Nano cosmetics protect the skin from ultraviolet rays by utilizing the ultraviolet protection function of nanoparticles or microparticles. Nanoparticles are particles having a total length of about 1 to 100 nm, and microparticles are particles having a total length of about 1 to 100 μm. Moreover, the particle | grains in the following description mean that it is not a plate shape or film shape etc., for example, and is a concept including the structure where a particle | grain is not necessarily a strict spherical shape.

  As UV-cut cosmetics using such nanoparticles or microparticles, for example, UV-cut cosmetics containing metal nanoparticles as a main component are well known (for example, see Non-Patent Document 1).

  For example, metal nanoparticles such as zinc oxide and titanium oxide are known to exhibit an effect of scattering ultraviolet rays. Therefore, by applying cosmetics containing these metal nanoparticles as a main component, skin can be protected from ultraviolet rays. Can be protected.

  However, at present, the safety of metal nanoparticles for living organisms has not been clearly proven, and there are still matters to be worried about, such as the effect on allergic individuals.

  Therefore, a UV-cut cosmetic is proposed in which nanoparticles or microparticles are produced from a disaggregated product of bacterial cellulose and the nanoparticles or microparticles derived from bacterial cellulose are the main components (see, for example, Patent Document 1). Here, the disaggregated material is a material component separated from the bacterial cellulose by a disaggregation treatment, that is, for example, by applying a mechanical external force or the like.

  As is well known, since bacterial cellulose is edible and tasteless and odorless, it is widely used in the fields of foods and cosmetics and has been confirmed to be safe for living bodies (for example, see Patent Document 2). Therefore, by using such bacterial cellulose-derived nanoparticles or microparticles, it is possible to provide UV-cut cosmetics with higher safety than when using the metal nanoparticles described above.

JP 2008-169188 A Japanese Patent Application Laid-Open No. 09-025302

Mari Tabuchi, Nature Biotechnology, 25 (4) 390-391 (2007)

  As described above, in recent years when carcinogenesis caused by ultraviolet rays has become a problem, in order to protect the skin from ultraviolet rays more reliably, for example, further improvement of ultraviolet ray protection functions such as UV cut cosmetics is desired.

  Accordingly, an object of the present invention is a microparticle derived from bacterial cellulose that is safe for living organisms and has a further improved ultraviolet protection function as a substance for protecting ultraviolet rays, for example, used in UV-cut cosmetics. An object of the present invention is to provide an anti-ultraviolet base as a mixture containing the particles and the microparticles.

  As a result of various studies by the inventor according to the present invention, the bacterial cellulose disaggregation product divided into individual pieces is packed with a viscous reagent (hereinafter also referred to as a viscous reagent), that is, individual disaggregation. It was found that the ultraviolet protection function of the bacterial cellulose-derived microparticles can be improved by individually wrapping each product with the viscous reagent as compared to the case where no packing is made.

  Therefore, in order to achieve the above object, the microparticle according to the present invention has the following characteristics.

  That is, the microparticle according to the present invention is formed by packing pieces of bacterial cellulose disaggregation with a viscous reagent.

  Moreover, the UV-proof base according to the present invention is a UV-proof base as a mixture containing the above-mentioned microparticles, and has the following characteristics.

  That is, the anti-ultraviolet base according to the present invention is composed of a mixture of pieces of bacterial cellulose disaggregation and water, and a viscous reagent, and includes microparticles. Are packed with a viscous reagent.

  Moreover, the UV-proof base according to the present invention is a UV-proof base as a mixture containing the above-mentioned microparticles, and has the following characteristics.

  That is, the UV-proof base according to the present invention includes microparticles in which individual pieces of bacterial cellulose disaggregation are packed with a viscous reagent and water.

  Moreover, the manufacturing method of the microparticle by this invention includes each process of the following 1st processes and 2nd processes.

  That is, first, in the first step, the bacterial cellulose disaggregation product is divided into individual pieces.

  Next, in a 2nd process, a piece-like disaggregation thing is packed with a viscous reagent by mixing a piece-like disaggregation thing with a viscous reagent.

  Moreover, the manufacturing method of the ultraviolet-resistant base by this invention mentioned above includes each process of the following 1st processes and 2nd processes.

  That is, first, in the first step, the disaggregated product is divided into individual pieces in a mixture of bacterial cellulose disaggregated material and water.

  Next, in the second step, the mixture and the viscous reagent are mixed to form microparticles in which the pieces of disaggregated material are packed with the viscous reagent. As a result, an anti-ultraviolet base is formed as a mixture containing microparticles in water.

  Since the microparticle according to the present invention is mainly composed of a disaggregated product of bacterial cellulose, it is safe for a living body. Therefore, the microparticles according to the present invention and the anti-ultraviolet base containing the microparticles can be used safely even when they are mixed with, for example, cosmetics and applied to human skin.

  In addition, as described above, the microparticles according to the present invention are configured by disaggregating bacterial cellulose fragments into individual pieces and packing them with a viscous reagent. As a result, the microparticles according to the present invention and the anti-ultraviolet base containing the same more effectively prevent the transmission of ultraviolet rays as compared with the conventional microparticles, that is, the disaggregated material that is not packed. Can do. (The details will be described in an embodiment described later).

  Further, in the method for producing microparticles and the method for producing an anti-ultraviolet base according to the present invention, the disaggregated bacterial cellulose disaggregated material is mixed with the viscous reagent, so that the disaggregated material can be easily obtained by the viscous reagent. As a result, it is possible to produce microparticles that are packed and, as a result, have an excellent UV protection function, and UV protection bases that contain the microparticles.

(A) And (B) is a figure for demonstrating 1st Embodiment of this invention, and shows a part of microparticles | fine-particles based on 1st Embodiment, and an ultraviolet-ray-proof base. It is a schematic diagram. (A) And (B) is a figure for demonstrating 1st Embodiment of this invention, A: 320 times the microparticle which concerns on 1st Embodiment, and a part of ultraviolet-proof base B: Photomicrographs taken at a magnification of 640 times. It is a figure which shows the light absorption spectrum of a UV protection base at the time of using glycerol as a viscous reagent, and a non-packing mixture. It is a figure which shows the light absorption spectrum of a UV protection base at the time of using a hyaluronic acid as a viscous reagent, and a non-packing mixture. (A) And (B) is a figure which shows each light absorption spectrum of the some ultraviolet-ray base in which the content density | concentration of microparticles differs when glycerol is used as a viscous reagent. It is a figure which shows the relationship between the density | concentration of the mixture used for preparation of a UV protection base, and the light absorbency in the ultraviolet region of the prepared UV protection base at the time of using glycerol as a viscous reagent. It is a figure which shows each light absorption spectrum of the some ultraviolet-ray base in which the containing density | concentration of microparticles differs when hyaluronic acid is used as a viscous reagent. It is a figure which shows the relationship between the density | concentration of the mixture used for preparation of an ultraviolet-resistant base, and the light absorbency in the ultraviolet region of the prepared ultraviolet-proof base in the case of using hyaluronic acid as a viscous reagent. It is the schematic diagram which showed roughly a part of disaggregation thing of bacterial cellulose obtained by the disaggregation process. It is the microscope picture which image | photographed a part of disaggregation thing of bacterial cellulose obtained by the disaggregation process at a magnification of 320 times. (A) And (B) is a figure which compares the size distribution of the disaggregated material before packing, and the microparticle after packing.

  Hereinafter, preferred embodiments of the present invention will be described with reference to the drawings. Each drawing merely schematically shows an example of the shape, size, and arrangement relationship of each component to the extent that the present invention can be understood. The embodiment described below is merely a preferred example, and therefore the configuration of the present invention is not limited to the illustrated configuration example described below, and deviates from the scope of the present invention. Obviously, many variations and modifications may be made without doing so. Further, for example, values such as concentration and volume described in the following description are values within a range in which the effect of the present invention can be achieved. However, it is not limited to this value.

<First Embodiment>
In the first embodiment, microparticles in which individual pieces of bacterial cellulose disaggregation are packed with a viscous reagent, and an anti-ultraviolet base as a mixture containing the microparticles will be described.

  1A and 1B are diagrams for explaining a first embodiment of the present invention, and schematically show a part of the microparticles and the UV-proof base according to the first embodiment. It is the schematic diagram shown in. 1A shows a bacterial cellulose disaggregation product (hereinafter also simply referred to as a disaggregation product) before packing with a viscous reagent, and FIG. 1B shows a disaggregation product packed with a viscous reagent. That is, the microparticles according to the first embodiment and the UV-proof base containing the same are shown.

  FIGS. 2A and 2B are views for explaining the first embodiment of the present invention. Part of the microparticles and the UV-proof base according to the first embodiment are shown. A: Photomicrographs taken at a magnification of 320 times and B: 640 times. 2A shows the disaggregated product of bacterial cellulose before being packed with the viscous reagent, and FIG. 2B shows the disaggregated product packed with the viscous reagent, that is, the first embodiment. Such microparticles and an anti-ultraviolet base containing the same are shown.

  In the microparticle 21 according to the first embodiment, the pieces 11a of the bacterial cellulose disaggregation 11 shown in FIGS. 1 (A) and 2 (A) are shown in FIGS. 1 (B) and 2 (B). As shown, it is packed with a viscous reagent. As will be described later, the bacterial cellulose disaggregation product 11 exists as a mixture with water, and the microparticles 21 also exist as a mixture with water. Therefore, a white portion in FIG. 1A indicates water (water containing a viscous reagent) 13 in which the disaggregated material 11 is mixed, and a white portion in FIG. 1B indicates that microparticles 21 are mixed. Water 25 (water containing a viscous reagent) is shown.

  Further, the UV-proof base 23 according to the first embodiment is configured as a mixture containing microparticles 21 in water 25.

  The microparticles 21 and the anti-ultraviolet base 23 including the micro-particles 21 are used as a main component of, for example, UV-cut cosmetics for preventing the transmission of ultraviolet rays. Therefore, as already described, the microparticle 21 needs to be safe with respect to a living body. Therefore, it is preferable that the disaggregation material 11 which is the main component of the microparticle 21 is β1,4-glucan disaggregated from, for example, bacterial cellulose derived from acetic acid bacteria. This β1,4-glucan has been conventionally used as a main component of foods, cosmetics, etc. and has been confirmed to be safe for living bodies (for example, see Patent Document 1 or Patent Document 2 described above).

  Here, the disaggregated material 11 is divided at the time of disaggregation from bacterial cellulose and exists as a plurality of individual pieces 11a. In addition, in FIG. 1 (A), these whole pieces of each disaggregated material 11a are shown as the disaggregated material 11. FIG. Moreover, the piece 11a of the disaggregated material 11 is a fibrous body including a partially lump-like portion 11b and a whisker-like portion 11c extending from the lump-like portion 11b (FIGS. 1A and 2). (See (A)). In FIG. 1A, three pieces 11a of the disaggregated material 11 divided into pieces are shown. FIG. 2 (A) shows a photomicrograph of one piece 11a out of the disaggregated material 11 divided into pieces.

  The bacterial cellulose derived from the acetic acid bacterium described above can be obtained by, for example, well-known stationary culture, stirring culture, or shaking culture. At this time, the obtained bacterial cellulose exists in a state of being contained in a gel-like mixture containing 99% or more of water. And the disaggregation thing 11 is formed by performing a disaggregation process with respect to the bacterial cellulose contained in this gel-like mixture. Therefore, the disaggregated material 11, that is, the individual piece 11 a exists as a mixture 15 with the water 13 as shown in FIGS. 1 (A) and 2 (A). In addition, about disaggregation of bacterial cellulose, it demonstrates in detail in the manufacturing method mentioned later.

  Moreover, the ultraviolet-proof base 23 is comprised by mixing this mixture 15 and the viscous reagent mentioned later. And in order to improve the ultraviolet-ray defense function of the ultraviolet-proof base 23, it is preferable to make the density | concentration of the disaggregated material 11 in this mixture 15 into a suitable value before mixing with a viscous reagent. Therefore, the mixture 15 may be diluted with water to adjust the concentration of the disaggregated material 11. Accordingly, the water 13 may include not only the water derived from bacterial cellulose described above but also water added to adjust the concentration.

  And the microparticle 21 which concerns on 1st Embodiment is comprised by packing the piece 11a of these division | segmentation disaggregation materials 11 with a viscous reagent, respectively.

  The viscous reagent is preferably a substance having a high viscosity in order to pack the pieces 11 a of the disaggregated material 11. More specifically, for example, glycerin or hyaluronic acid is preferably used as the viscous reagent.

  In the first embodiment, the viscosity of the viscous reagent is preferably defined by the concentration of the viscous reagent in the UV-proof base 23 including the formed microparticles 21. And it is preferable to adjust the density | concentration of the viscous reagent from which the light absorbency with respect to the ultraviolet-ray of the ultraviolet-protecting base 23 becomes favorable.

  For example, when glycerin is used as a viscous reagent, the concentration of glycerin in the UV-proof base 23 is set to a weight concentration in order to obtain the best UV-proof effect, that is, to maximize the absorbance to UV rays. It is preferably 4.5%. For this purpose, the UV-proof base 23 is preferably constituted by mixing the mixture 15 and, for example, a 9% glycerin aqueous solution in a weight concentration at a volume ratio of 1: 1.

  Similarly, for example, when hyaluronic acid is used as a viscous reagent, the hyaluronic acid concentration in the UV-proof base 23 is 0.0005% in terms of weight concentration in order to obtain the best UV protection effect. Is preferable. For this purpose, the UV-proof base 23 is preferably constituted by mixing the mixture 15 and, for example, a 0.001% hyaluronic acid aqueous solution in a weight concentration at a volume ratio of 1: 1.

  Moreover, as already demonstrated, the piece 11a of the disaggregation thing 11 exists as the mixture 15 with the water 13, as shown to FIG. 1 (A) and FIG. 2 (A). And in this 1st Embodiment, the ultraviolet-ray base 23 is prepared by mixing this mixture 15 and a viscous reagent, and also by this mixing, the piece 11a is packed with a viscous reagent, and a microparticle 21 is formed. Therefore, the UV-proof base 23 is configured as a mixture of the water 25 derived from the mixture 15 and the microparticles 21.

  Here, the water 25 is a portion of the viscous reagent that does not participate in the packing, and when the viscous reagent is diluted with water to adjust its concentration, that is, when it is mixed in the state of an aqueous solution. In some cases, water derived from this viscous reagent aqueous solution is contained.

  The inventor of this application conducted an experiment to confirm the effect of preventing the transmission of ultraviolet rays, that is, the ultraviolet ray preventing effect, of the microparticles 21 according to the first embodiment and the ultraviolet ray preventing base material 23 including the same. In this experiment, the UV-proof base 23 according to this embodiment, that is, the UV-proof base 23 including the microparticles 21, and the disaggregated material that is not packed with a viscous reagent (hereinafter also referred to as non-packed disaggregated material). And a mixture of water (that is, for example, the mixture 15 shown in FIGS. 1A and 2A: hereinafter also referred to as an unpacked mixture), the respective light absorption spectra were measured, and the measurement results were compared. .

  FIG. 3 is a diagram showing the results of this experiment, and is a diagram showing the light absorption spectra of the UV-proof base 23 and the non-packing mixture. In FIG. 3, the vertical axis indicates the relative value of absorbance, and the horizontal axis indicates the wavelength of the irradiated light in nm units.

  In the experiment in which the light absorption spectrum of FIG. 3 was obtained, a mixture containing 0.4% of unpacked disaggregate in weight concentration in water was used as a sample of the unpacked mixture. In addition, as a sample of the UV-proof base 23, a mixture 15 containing the disaggregation material 11 having a weight concentration of 0.4% equivalent to the sample of the non-packing mixture and an aqueous solution of glycerin as a viscous reagent are 1: 1. A sample prepared by mixing at a volume ratio of was used. In this experiment, the concentration of the glycerin aqueous solution used was 9% in terms of weight concentration.

  In FIG. 3, a curve 31 shows a light absorption spectrum of the sample of the UV-proof base 23. Curve 33 shows the light absorption spectrum of the sample of the unpacked mixture. A curve 35 shows the light absorption spectrum of the glycerin aqueous solution used when preparing the sample of the UV-proof base 23.

  As is clear from the results of FIG. 3, the UV-proof base 23 including the microparticles 21 packed with the glycerin aqueous solution is at least 220 in comparison with the non-packing mixture including the non-packing disaggregated material that is not packed. Absorbance is increased over a wavelength range of ˜700 nm. In particular, in the ultraviolet protection base 23, a high peak 31a can be confirmed in the ultraviolet region near 250 to 270 nm.

  Further, the light absorption spectrum of the glycerin aqueous solution alone, that is, the curve 35, was significantly lower in absorbance than the UV-proof base 23 and the non-packing mixture over the entire wavelength region. Therefore, it can be seen that the increase in the absorbance of the UV-proof base 23 is not due to the glycerin aqueous solution alone but to the microparticles 21 contained in the UV-proof base 23.

  From this result, it was confirmed that the microparticles 21 obtained by packing the disaggregated material 11 with glycerin and the UV-proof base 23 including the same were effective in protecting the transmission of UV rays.

  In addition, the inventor of this application also performed an experiment similar to the experiment shown in FIG. 3 described above for a sample of the anti-UV base 23 prepared using hyaluronic acid as a viscous reagent. That is, the light absorption spectra of the sample of the UV-proof base 23 containing the microparticles 21 packed with hyaluronic acid and the sample of the non-packing mixture were measured, and the measurement results were compared.

  FIG. 4 is a diagram showing the results of this experiment, and is a diagram showing the light absorption spectra of the UV-proof base 23 and the non-packing mixture. In FIG. 4, the vertical axis indicates the relative value of the absorbance, and the horizontal axis indicates the wavelength of the irradiated light in units of nm.

  In the experiment for obtaining the light absorption spectrum of FIG. 4, a mixture containing 0.4% of unpacked disaggregated material in water in water was used as a sample of the unpacked mixture. Further, as a sample of the UV-proof base 23, a mixture 15 containing the disaggregated material 11 having a weight concentration of 0.4%, which is equivalent to the sample of the non-packing mixture (see FIG. 1 (A) and FIG. 2 (A)). And a sample prepared by mixing an aqueous solution of hyaluronic acid as a viscous reagent at a volume ratio of 1: 1. In this experiment, the concentration of the used hyaluronic acid aqueous solution was 0.001% in terms of weight concentration.

  In FIG. 4, a curve 41 indicates the light absorption spectrum of the sample of the UV-proof base 23. Curve 43 shows the light absorption spectrum of the sample of the unpacked mixture. A curve 45 represents a light absorption spectrum of the aqueous hyaluronic acid solution used in preparing the UV-proof base 23.

  As is clear from the results of FIG. 4, the anti-ultraviolet base 23 including the microparticles 21 packed with the hyaluronic acid aqueous solution is at least as compared with the non-packing mixture including the non-packing disaggregated material that is not packed. Absorbance increases over a wavelength range of 220-750 nm. And also in the ultraviolet region near 250-270 nm, it turns out that the light absorbency of the ultraviolet-proof base 23 is large compared with a non-packing mixture.

  Moreover, the light absorption spectrum of the hyaluronic acid aqueous solution alone, that is, the curve 45, was significantly lower in absorbance than the anti-UV base 23 and the non-packing mixture over the entire wavelength region. Therefore, it can be seen that the increase in the absorbance of the UV-proof base 23 is not due to the hyaluronic acid aqueous solution alone but to the microparticles 21 contained in the UV-proof base 23.

  From this result, as in the case of using glycerin as a viscous reagent, the microparticles 21 obtained by packing the disaggregated material 11 with hyaluronic acid and the UV-proof base 23 containing the same prevent the penetration of UV rays. It was confirmed that it was effective.

  Next, the inventor of this application applied the anti-ultraviolet effect of the anti-ultraviolet base 23 according to the first embodiment and the microparticles 21 in the anti-ultraviolet base 23 over a wavelength range of 220 to 750 nm. Experiments were conducted to confirm the relationship with the concentration of In this experiment, a plurality of UV-proof bases 23 containing microparticles 21 having different concentrations were prepared as samples. That is, these samples have a volume ratio of a mixture 15 (see FIGS. 1 (A) and 2 (A)) containing disaggregation material 11 in water 15 at different weight concentrations and an aqueous solution of a viscous reagent. Prepared by mixing 1: 1. Then, the respective light absorption spectra of the samples of the UV-proof base 23 having different weight concentrations were measured, and the measurement results were compared.

  5 (A) and 5 (B) are diagrams showing the results of this experiment, showing the respective light absorption spectra of a plurality of UV protection bases 23 having different concentrations of microparticles 21. FIG. In FIG. 5, the vertical axis shows the relative value of absorbance, and the horizontal axis shows the wavelength of the irradiated light in nm units.

  In FIG. 5 (A), a curve 51 indicates a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.036% of the disaggregated material 11 in weight concentration with an aqueous solution of glycerin as a viscous reagent. The light absorption spectrum of is shown. A curve 52 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 0.090% and the glycerin aqueous solution. A curve 53 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 0.18% and the glycerin aqueous solution. A curve 54 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.36% of the disaggregated material 11 by weight and the glycerin aqueous solution.

  In FIG. 5B, a curve 55 indicates the light absorption of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 0.36% and the glycerin aqueous solution. The spectrum, that is, the same light absorption spectrum as the curve 54 in FIG. A curve 56 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 0.72% and the glycerin aqueous solution. A curve 57 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.96% of the disaggregated material 11 in weight concentration and the glycerin aqueous solution. A curve 58 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 1.44% and the glycerin aqueous solution. A curve 59 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 2.88% and the glycerin aqueous solution.

  In addition, in the experiment which obtained the light absorption spectrum of this FIG. 5 (A) and (B), in preparing the ultraviolet-ray prevention base 23 as each above-mentioned sample, 9% of glycerin aqueous solution was used in weight concentration. .

  From the result of FIG. 5 (A), the higher the concentration of the disaggregated material 11 in the mixture 15 used for the preparation of the UV-proof base 23, the more the wavelength range of the UV-proof base 23 formed is included. It can be seen that the absorbance increases.

  However, from the result of FIG. 5 (B), when the weight concentration of the disaggregated material 11 in the mixture 15 is 0.72% or more, compared with the case where the weight concentration of the disaggregated material 11 is 0.36%. Thus, it can be seen that the absorbance of the formed UV-proof base 23 becomes small.

  Here, FIG. 6 is a diagram showing the relationship between the concentration of the mixture 15 used for the preparation of the UV-proof base 23 and the absorbance of the prepared UV-proof base 23 in the ultraviolet region. In FIG. 6, the absorbances of light of wavelengths in the ultraviolet region, more specifically, of light having a wavelength of 250 nm in the absorption spectra of the samples of FIGS. 5A and 5B are plotted.

  In FIG. 6, the vertical axis represents the relative value of the absorbance with respect to light of 250 nm in the absorption spectrum of each UV-proof base 23 in FIGS. 5A and 5B, and the horizontal axis represents glycerin as a viscous reagent. The concentration of the disaggregated material 11 in the mixture 15 before mixing with the aqueous solution is graduated in weight percent.

  In FIG. 6, point 61 is a mixture of the mixture 15 containing 0.036% of the disaggregated material 11 in weight concentration and the aqueous solution of glycerin as a viscous reagent based on the curve 51 in FIG. It is the point which plotted the light absorbency with respect to the light of 250 nm of the sample of the ultraviolet-ray base 23 obtained in this way. The point 62 is based on the curve 52 in FIG. 5A, and the light of 250 nm of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.090% of the disaggregated material 11 and the glycerin aqueous solution. It is the point which plotted the light absorbency with respect to. The point 63 is the light of 250 nm of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.18% of the disaggregated material 11 and the glycerin aqueous solution based on the curve 53 in FIG. It is the point which plotted the light absorbency with respect to. The point 64 is the ultraviolet ray-proof obtained by mixing the mixture 15 containing 0.36% of the disaggregated material 11 and the glycerin aqueous solution based on the curve 54 in FIG. 5A or the curve 55 in FIG. 5B. It is the point which plotted the light absorbency with respect to the light of 250 nm of the sample of the base material 23. FIG. The point 65 is the light of 250 nm of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.72% of the disaggregated material 11 and the glycerin aqueous solution based on the curve 56 in FIG. It is the point which plotted the light absorbency with respect to. Point 66 is the light of 250 nm of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.96% of the disaggregated material 11 and the glycerin aqueous solution based on the curve 57 in FIG. 5B. It is the point which plotted the light absorbency with respect to. A point 67 is a light of 250 nm of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 1.44% of the disaggregated material 11 and the glycerin aqueous solution based on the curve 58 in FIG. 5B. It is the point which plotted the light absorbency with respect to. The point 68 is based on the curve 59 in FIG. 5B, and the light of 250 nm of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 2.88% of the disaggregated material 11 and the glycerin aqueous solution. It is the point which plotted the light absorbency with respect to.

  The curve 60 is an approximate line that is approximately determined based on the plotted points 61, 62, 63, 64, 65, 66, 67, and 68 described above.

  From the results of FIG. 6, it is understood that the absorbance in the ultraviolet region of 250 nm of the UV-proof base 23 is maximized when the weight concentration of the disaggregated material 11 in the mixture 15 used for the preparation is about 0.36%. It was. From the above, the UV-proof base 23 according to the first embodiment is prepared by mixing the mixture 15 in which the disaggregated material 11 of 0.36% in weight concentration is mixed with the glycerin aqueous solution. preferable.

  5A, FIG. 5B, and FIG. 6, the absorbance of each anti-UV base 23 prepared using the mixture 15 of each concentration, and the non-packing mixture shown in FIG. 3 or FIG. 5A, FIG. 5B, and FIG. 6 show that each UV-proof base 23 used as a sample in the experiment has higher absorbance than the non-packing mixture. . Therefore, the UV-proof base 23 according to the first embodiment includes the mixture 15 in which the disaggregation material 11 is mixed in a weight concentration range of 0.036 to 2.88% and the glycerin in a weight concentration of 9%. By mixing the aqueous solution with a volume ratio of 1: 1, it is possible to obtain a greater absorbance than the non-packing mixture.

  Further, the inventor of the present invention conducted the same experiment as that shown in FIGS. 5 (A), 5 (B), and 6 described above by using hyaluronic acid as a viscous reagent. Also went on. That is, in this experiment, the sample 15 was prepared by mixing the mixture 15 containing the disaggregated material 11 in the water 15 with different weight concentrations and the aqueous solution of hyaluronic acid as a viscous reagent in a volume ratio of 1: 1. did. Then, the respective light absorption spectra of the samples of the UV-proof base 23 having different weight concentrations were measured, and the measurement results were compared.

  FIG. 7 is a diagram showing the results of this experiment, and is a diagram showing each light absorption spectrum of a plurality of samples of the UV-proof base 23 having different microparticle 21 content concentrations. In FIG. 7, the vertical axis indicates the relative value of absorbance, and the horizontal axis indicates the wavelength of the irradiated light in nm units.

  In FIG. 7, a curve 71 shows the light of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 0.036% and the aqueous solution of hyaluronic acid as a viscous reagent. An absorption spectrum is shown. A curve 72 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 0.090% and the hyaluronic acid aqueous solution. A curve 73 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 0.18% and the hyaluronic acid aqueous solution. A curve 74 shows a light absorption spectrum of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the disaggregated material 11 having a weight concentration of 0.36% and the hyaluronic acid aqueous solution.

  In the experiment for obtaining the light absorption spectrum of FIG. 7, a 0.001% hyaluronic acid aqueous solution was used in terms of weight concentration in preparing the UV-proof base 23 as each sample described above.

  From the results of FIG. 7, even when hyaluronic acid is used as the viscous reagent, the higher the concentration of the disaggregated material 11 in the mixture 15 used for the preparation of the UV-proof base 23, the higher the UV-proof base 23 formed. It can be seen that the absorbance including the wavelength region in the ultraviolet region increases.

  By the way, in view of the results of the experiments shown in FIGS. 5 (A), 5 (B), and 6 described above, even when hyaluronic acid is used as the viscous reagent, the mixture is the same as when glycerin is used. 15 is considered to have a peak at which the absorbance of the UV-proof base 23 is maximum.

  Therefore, the inventor of the present application contains the disaggregated material 11 at a concentration higher than 0.36%, the mixture 15 having different concentrations, and the hyaluronic acid having a weight concentration of 0.001% as described above. Absorbance was measured for a plurality of UV-proof bases 23 prepared by mixing the aqueous solution at a volume ratio of 1: 1. Then, based on this result and the absorption spectrum of each sample in FIG. 7, FIG. 8 similar to FIG. 6 described below is created, and the concentration of the mixture 15 and the absorbance in the ultraviolet region of the UV-proof base 23 are prepared. And confirmed the relationship.

  FIG. 8 is a diagram showing the relationship between the concentration of the mixture 15 used for the preparation of the UV-proof base 23 and the absorbance of the prepared UV-proof base 23 in the ultraviolet region. In FIG. 8, the absorbance of the ultraviolet spectrum wavelength in the absorption spectrum of each sample in FIG. 7, more specifically, each absorbance with respect to the wavelength of 250 nm, and the disaggregate 11 at a concentration higher than 0.36%. Each light absorbency with respect to the wavelength light of 250 nm of the some ultraviolet-protecting base 23 prepared using the mixture 15 containing is plotted.

  In FIG. 8, the vertical axis represents the relative value of absorbance with respect to light of 250 nm in the absorption spectrum of each UV protection base 23, and the horizontal axis represents the mixture 15 before being mixed with an aqueous solution of hyaluronic acid as a viscous reagent. The concentration of the disaggregated material 11 is graduated in weight percent.

  Further, in FIG. 8, point 81 is obtained by mixing the mixture 15 containing 0.036% of the disaggregated material 11 in weight concentration and the aqueous solution of hyaluronic acid as a viscous reagent based on the curve 71 in FIG. In addition, the absorbance of the sample of the UV-proof base 23 with respect to light of 250 nm is plotted. The point 82 is the absorbance of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.090% of the disaggregated material 11 and the hyaluronic acid aqueous solution with respect to light of 250 nm based on the curve 72 in FIG. Is a point plotted. Point 83 is the absorbance of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.18% of the disaggregated material 11 and the hyaluronic acid aqueous solution with respect to light of 250 nm based on the curve 73 in FIG. Is a point plotted. The point 84 is the absorbance of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.36% of the disaggregated material 11 and the hyaluronic acid aqueous solution with respect to light of 250 nm based on the curve 74 in FIG. Is a point plotted. Point 85 is a point in which the absorbance of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.40% of the disaggregated material 11 and the hyaluronic acid aqueous solution with respect to light of 250 nm is plotted. Point 86 is a point in which the absorbance of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.42% of the disaggregated material 11 and the hyaluronic acid aqueous solution with respect to light of 250 nm is plotted. Point 87 is a point in which the absorbance of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing the 0.50% disaggregate 11 and the hyaluronic acid aqueous solution with respect to light of 250 nm is plotted. Point 88 is a point in which the absorbance of the sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.65% of the disaggregated material 11 and the hyaluronic acid aqueous solution with respect to light of 250 nm is plotted. Point 89 is a point in which the absorbance of a sample of the UV-proof base 23 obtained by mixing the mixture 15 containing 0.96% of the disaggregated material 11 and the hyaluronic acid aqueous solution with respect to light of 250 nm is plotted.

  A curve 80 is an approximate line that is approximately determined based on the plotted points 81, 82, 83, 84, 85, 86, 87, 88, and 89 described above.

  From the results of FIG. 8, the absorbance in the ultraviolet region of 250 nm of the UV-proof base 23 is maximum when the weight concentration of the disaggregated material 11 in the mixture 15 used for the preparation is about 0.36 to 0.40%. I found out that From the above, when hyaluronic acid is used as the viscous reagent, the UV-proof base 23 adjusts the weight concentration of the disaggregated material 11 in the mixture 15 to 0.36 to 0.40% and mixes with hyaluronic acid. Is preferred.

  7 and 8 are compared with the absorbance of each anti-UV base 23 prepared using the mixture 15 of each concentration and the absorbance of the non-packing mixture shown in FIG. 3 or FIG. It can be seen that each UV-proof base 23 used as a sample in the experiment according to FIG. 8 has a higher absorbance for light having a wavelength of 250 nm than that of the unpacked mixture. Therefore, in the UV-proof base 23 according to the first embodiment, the mixture 15 in which the disaggregation material 11 is mixed in the weight concentration range of 0.036 to 0.96% and the weight concentration of 0.001%. By mixing the aqueous hyaluronic acid solution with a volume ratio of 1: 1, it is possible to obtain a large UV protection effect against light in the ultraviolet region of 250 nm as compared with the non-packing mixture.

  As described above, the microparticles 21 according to the first embodiment are safe for a living body because they are mainly composed of the bacterial cellulose disaggregation material 11. Therefore, the microparticles 21 according to the first embodiment and the UV-proof equipment 23 containing the microparticles 21 can be safely used even when they are mixed with cosmetics and applied to human skin, for example.

  In addition, as described above, the microparticles 21 according to the first embodiment are configured by dividing the bacterial cellulose disaggregation material 11 into individual pieces and packing them with a viscous reagent. As a result, the microparticles 21 according to the first embodiment can more effectively prevent the transmission of ultraviolet rays as compared with the disaggregated material that is not packed.

  Therefore, by using the microparticles 21 or the UV-proof base 23 containing the microparticles 21 mixed with, for example, cosmetics, the transmission of ultraviolet rays can be prevented more securely and more securely than before. Can do.

  Next, a method for manufacturing the microparticles 21 and the UV-proof base 23 (see FIGS. 1B and 2B) according to the first embodiment will be described.

  The microparticles 21 according to the first embodiment described above are simultaneously formed while being mixed with the UV-proof base 23 when the UV-proof base 23 is formed. Therefore, in this embodiment, the manufacturing method of the UV-proof base 23 will be mainly described, and the microparticles 21 formed by being included in the UV-proof base 23 will also be described.

  The method for producing the UV-proof base 23 includes a first step and a second step. Hereinafter, each step will be described in order from the first step.

  First, in the first step, the disaggregated product is divided into individual pieces in a mixture of disaggregated bacterial cellulose and water.

  As already described, in the first embodiment, bacterial cellulose derived from acetic acid bacteria is used as bacterial cellulose. And this bacterial cellulose is obtained by carrying out well-known stationary culture or stirring culture of acetic acid bacteria, and is in a gel state.

  And a disaggregation thing is obtained by performing a well-known disaggregation process with respect to this bacterial cellulose. That is, for example, by applying this bacterial cellulose to a mixer at 20000 rpm for 5 to 40 minutes, a disaggregated product is obtained. In addition, when performing the disaggregation process by a mixer for 5 minutes or more, it is preferable to carry out at intervals of 30 minutes every 5 minutes. In this disaggregation process, the disaggregated material is obtained by being divided into individual pieces.

  Here, the disaggregated material obtained by the disaggregation process includes a plurality of types of disaggregated materials such as β1,4-glucan and β1,3-glucan. As already explained, in the first embodiment, β1,4-glucan is used as the disaggregation product. Therefore, in order to selectively purify β1,4-glucan from the obtained disaggregated material, for example, a 0.1N aqueous sodium hydroxide solution is used for the disaggregated material obtained by the above disaggregation treatment. Washing is performed 10 times at 20 ° C. for 20 minutes, and further washing is performed 10 times at 105 ° C. for 20 minutes using distilled water. As a result, β1,4-glucan can be selectively obtained from the above-mentioned multiple types of disaggregated products.

  Moreover, the above-mentioned bacterial cellulose exists in the state contained in the gel-like mixture containing 99% or more of water. Therefore, the disaggregated product obtained by disaggregating this bacterial cellulose, that is, β1,4-glucan is obtained as a mixture with water.

  Here, FIG. 9 is a schematic diagram schematically showing a disaggregated product of bacterial cellulose obtained by this disaggregation treatment. FIG. 10 is a photomicrograph of the disaggregated material taken at a magnification of 320 times.

  As shown in FIG. 9 and FIG. 10, β1,4-glucan as a disaggregation product obtained by the disaggregation process described above includes a partially lump-like portion 11b and a whisker-like portion 11c extending from the lump-like portion 11b. A plurality of pieces of disaggregated material 11a (see FIGS. 1A and 2A) are entangled with each other. And the disaggregation thing 11 as the whole of the several piece 11a exists as the mixture 19 with the water 17 derived from bacterial cellulose mentioned above. In FIG. 9, the white portion in the figure is water 17 mixed with the disaggregated material 11.

  Here, as described above, in the microparticles to be manufactured and the UV-proof base containing the microparticles, in order to obtain a good UV-proof effect, the concentration of the disaggregated material 11 in the mixture 19 is adjusted to a suitable value. It is preferable to keep it.

  More specifically, in the subsequent second step, when packing the disaggregated material 11 with, for example, glycerin as a viscous reagent, the concentration of the disaggregated material 11 in the mixture is 0.036 to 2.88 in terms of weight concentration. %, More preferably 0.36%. Further, when packing the disaggregated material 11 with, for example, hyaluronic acid as a viscous reagent, the concentration of the disaggregated material 11 in the mixture is 0.036 to 0.96% in terms of weight concentration, more preferably 0.36. It is preferable to adjust to ˜0.40%.

  Therefore, in this first step, it is preferable to dilute the mixture 19 with water. As a result, as shown in FIG. 1 (A) and FIG. 2 (A), each piece 11a of the bacterial cellulose disaggregation 11 divided into individual pieces is floating in the water 13 and these disaggregation. A mixture 15 of objects 11 and pieces 11a is obtained.

  Next, in a 2nd process, the mixture 15 shown to FIG. 1 (A) and FIG. 2 (A) is mixed with a viscous reagent. As a result, the pieces of disaggregated material 11 existing in the mixture 15, that is, the pieces 11a, are mixed with the viscous reagent, and the pieces 11a are packed with the viscous reagent to form the microparticles 21. At the same time, the UV-proof base 23 as a mixture containing the microparticles 21 in the water 25 is formed (see FIGS. 1B and 2B).

  The viscous reagent is a substance having a high viscosity. In the first embodiment, for example, glycerin or hyaluronic acid is preferably used as the viscous reagent.

  In addition, as described above, it is preferable to suitably adjust the concentration of the viscous reagent in the UV-proof base 23 so that the absorbance of the formed UV-proof base 23 with respect to the ultraviolet light becomes good. For this purpose, in this second step, the viscous reagent is diluted with water to obtain an aqueous solution, and the volume ratio of the mixture 15 to be mixed and the viscous reagent is adjusted.

  More specifically, for example, when glycerin is used as a viscous reagent, the concentration of glycerin in the UV protection base 23 is set to obtain a good UV protection effect, that is, to maximize the absorbance to UV rays. The weight concentration is preferably adjusted to 4.5%. For this purpose, in this second step, it is preferable to mix the mixture 15 and a 9% glycerin aqueous solution in a weight concentration at a volume ratio of 1: 1 to form an anti-ultraviolet base.

  For example, when hyaluronic acid is used as a viscous reagent, the concentration of hyaluronic acid in the UV-proof base 23 is adjusted to 0.0005% in terms of weight concentration in order to obtain a good UV-proof effect. Is preferred. Therefore, in this second step, it is preferable to form a UV-proof base by mixing the mixture 15 and a 0.001% hyaluronic acid aqueous solution in a weight concentration at a volume ratio of 1: 1.

  Moreover, it is preferable to mix the mixture 15 and a viscous reagent by stirring using a mixer etc., for example.

  The water 25 includes water 13 (see FIGS. 1 (A) and 2 (A)) contained in the mixture 15, a portion of the viscous reagent that does not participate in packing, and the viscous reagent has its concentration. When diluted with water and used to adjust the viscosity, water derived from this viscous reagent aqueous solution is included.

  In the second step, the UV-proof base 23 including the water 25 and the microparticles 21 formed by packing the pieces 11a of the bacterial cellulose disaggregation 11 with a viscous reagent is formed.

  The inventor of this application uses the pieces 11a of the disaggregated material 11 obtained in the first step described above (see FIGS. 1A and 2A) and the microparticles 21 obtained in the second step described above ( 1B and FIG. 2B), that is, with respect to the microparticles 21 manufactured in this embodiment, the size of each particle is measured, and the result is used to determine the pieces 11a of the disaggregated material. The size distribution and the size distribution of the microparticles 21 were compared.

  FIGS. 11A and 11B are diagrams for comparing the size distribution between the disaggregated material before packing and the microparticles obtained by packing the disaggregated material. More specifically, FIG. 11 (A) shows the size distribution of the pieces 11a contained in the disaggregated material before packing, that is, the mixture 15 shown in FIGS. 1 (A) and 2 (A). Yes. FIG. 11B shows the microparticles produced by the microparticle production method according to this embodiment, that is, the microparticles included in the UV-proof base 23 shown in FIGS. 1B and 2B. The size distribution of the particles 21 is shown. In FIG. 11A, the vertical axis indicates the number of pieces 11a of the disaggregated material, and the horizontal axis indicates the size of the pieces 11a of the disaggregated material in units of μm. In FIG. 11B, the vertical axis indicates the number of microparticles 21 and the horizontal axis indicates the size of the microparticles 21 in units of μm.

  In the size distribution according to FIG. 11A, as the above-described disaggregation process, a disaggregation product 11 obtained by applying bacterial cellulose to a mixer for 40 minutes was used, and the individual piece 11a was used as a sample.

  And the micrograph as shown to FIG. 2 (A) was image | photographed about the several piece 11a as this sample. Furthermore, about 20 pieces were randomly selected from the photographed pieces 11a, and the total length of each piece 11a in these photographs was measured using a measure. Then, based on the measured values, the original total length of each piece 11a was calculated based on the magnification of the microscope when these photographs were taken, thereby obtaining the size of the piece 11a.

  Moreover, in the size distribution according to FIG. 11B, the pieces 11a of the disaggregated material obtained from the size distribution of FIG. 11A are packed with a viscous reagent, and the obtained microparticles 21 are used as samples. In addition, the microparticle 21 which concerns on this FIG.11 (B) is 9.0% by weight aqueous solution of the mixture 15 containing the disaggregation material 11 0.4% in weight concentration, and glycerin as a viscous reagent. Were mixed at a volume ratio of 1: 1.

  And about the some microparticle 21 as this sample, the microscope picture as shown to FIG. 2 (B) was image | photographed. Furthermore, about 20 sheets were randomly selected from the photographed plural microparticles 21 and the total length of each microparticle 21 in these photographs was measured using a measure. And based on the magnification of the microscope when these photographs were taken from this measurement value, the original total length of each microparticle 21 was calculated to obtain the size of the microparticle 21.

  As is clear from FIGS. 11A and 11B, the particle size of the pieces 11a of the disaggregated material 11 before packing is widely distributed over a range of about 15 to 150 μm. On the other hand, it can be seen that the size of the microparticles 21 after packing is concentrated in the vicinity of about 25 μm. Therefore, the microparticles 21 obtained by this embodiment have a reduced size variation, that is, are homogeneous compared to the individual pieces 11a of the disaggregated material 11 before being packed.

  Furthermore, the inventor according to this application has determined the size of the individual pieces 11a of the disaggregated material 11 and the microparticles 21 used as the samples according to these figures from the size distributions shown in FIGS. Average values and standard deviations were determined.

  As a result, the average value and standard deviation of the size of the pieces 11a of the disaggregated material 11 according to FIG. 11A are 73 ± 28 μm, and the average value and standard of the size of the microparticles 21 according to FIG. The deviation was 25 ± 6 μm. As is clear from these results, it was confirmed that the size of the microparticles 21 obtained can be uniformly adjusted by packing the pieces 11a of the disaggregated material 11 with a viscous reagent.

  As described above, in the method for producing microparticles and the method for producing an anti-ultraviolet base according to the first embodiment, the bacterial cellulose disaggregation product 11 divided into pieces is mixed with a viscous reagent, The disaggregated material 11 is simply packed with a viscous reagent, and as a result, the microparticles 21 having an excellent UV protection function and the UV-proof base 23 containing the microparticles 21 can be manufactured.

  As is well known in the art, in the cosmetic manufacturing process, the particle size of the particles constituting the cosmetic may be adjusted in order to adjust the color and tone. And as shown in FIG.11 (B), the size of the microparticle 21 manufactured by the manufacturing method of this 1st Embodiment is controlled uniformly. Accordingly, when the microparticles 21 manufactured by the manufacturing method of the first embodiment or the UV-proof base 23 containing the microparticles 21 are mixed and used in, for example, cosmetics, a desired color and tone By adjusting the size of the microparticles 21 according to the above, it is possible to mix without impairing the color or tone of the cosmetic. Note that the size of the microparticles 21 is adjusted after the packing by adjusting the time during which the bacterial cellulose is applied to the mixer and adjusting the size of the pieces 11a of the disaggregated material 11 before packing. The uniform size of the microparticles 21 can be adjusted.

11: Disaggregated material 11a: Individual piece (an individual piece of disaggregated material)
13, 17, 25: Water 15, 19: Mixture 21: Microparticle 23: UV-proof base

Claims (30)

A microparticle comprising a piece of disaggregated bacterial cellulose packed with hyaluronic acid . The microparticle according to claim 1 ,
The microparticle, wherein the disaggregation product is β1,4-glucan. It consists of a mixture of pieces of bacterial cellulose disaggregation and water and a viscous reagent, and contains microparticles,
The anti-ultraviolet base, wherein the microparticles are formed by packing the pieces of the disaggregated material with the viscous reagent. The anti-ultraviolet base according to claim 3 ,
The UV-proof base, wherein the viscous reagent is glycerin. The anti-ultraviolet base according to claim 4 ,
The anti-ultraviolet base, wherein the concentration of the disaggregated material in the mixture is 0.036 to 2.88% in terms of weight concentration. The UV-proof base according to claim 5 ,
The anti-ultraviolet base, wherein the concentration of the disaggregated material in the mixture is 0.36% in terms of weight concentration. It is an ultraviolet-resistant base as described in any one of Claims 4-6 ,
The ultraviolet-resistant base, wherein the concentration of the glycerin in the ultraviolet-proof base is 4.5% by weight. The anti-ultraviolet base according to claim 3 ,
The UV-resistant base, wherein the viscous reagent is hyaluronic acid. The anti-ultraviolet base according to claim 8 ,
The anti-ultraviolet base, wherein the concentration of the disaggregated material in the mixture is 0.036 to 0.96% in terms of weight concentration. The UV-proof base according to claim 9 ,
The anti-ultraviolet base, wherein the concentration of the disaggregated material in the mixture is 0.36 to 0.40% by weight. It is an ultraviolet-protecting base as described in any one of Claims 8-10 ,
The UV-protecting base, wherein the concentration of the hyaluronic acid in the UV-protecting base is 0.0005% by weight. The UV-proof base according to claim 3 , wherein
The anti-ultraviolet base, wherein the disaggregation product is β1,4-glucan. An anti-ultraviolet base comprising microparticles in which pieces of bacterial cellulose disaggregation are packed with a viscous reagent and water. The anti-ultraviolet base according to claim 13 ,
The UV-proof base, wherein the viscous reagent is glycerin. The anti-ultraviolet base according to claim 14 ,
The ultraviolet-resistant base, wherein the concentration of the glycerin in the ultraviolet-proof base is 4.5% by weight. The anti-ultraviolet base according to claim 13 ,
The UV-resistant base, wherein the viscous reagent is hyaluronic acid. The anti-ultraviolet base according to claim 16 ,
The UV-protecting base, wherein the concentration of the hyaluronic acid in the UV-protecting base is 0.0005% by weight. The UV-proof base according to claim 13 , wherein
The anti-ultraviolet base, wherein the disaggregation product is β1,4-glucan. A first step of dividing the bacterial cellulose disaggregation into individual pieces;
A method for producing microparticles, comprising: a second step of packing the piece-like disaggregated material with hyaluronic acid by mixing the piece-like disaggregated material with hyaluronic acid . The microparticle production method according to claim 19 ,
A method for producing microparticles, wherein β1,4-glucan is used as the disaggregation product. A first step of dividing the disaggregated product into individual pieces in a mixture of bacterial cellulose disaggregated product and water;
By mixing the mixture with a viscous reagent, the piece-like disaggregated material forms microparticles packed with the viscous reagent, and forms an anti-ultraviolet base as a mixture containing the microparticles in water. A method for producing an ultraviolet-resistant base, comprising a second step. It is a manufacturing method of the ultraviolet protection base according to claim 21 ,
A method for producing a UV-proof base, characterized in that glycerin is used as the viscous reagent. It is a manufacturing method of the ultraviolet protection base according to claim 22 ,
The method for producing a UV-proof base, wherein the concentration of the disaggregated material in the mixture is adjusted to 0.036 to 2.88% in terms of weight concentration. It is a manufacturing method of the ultraviolet protection base according to claim 23 ,
A method for producing a UV-proof base, characterized in that the concentration of the disaggregated material in the mixture is adjusted to 0.36% in terms of weight concentration. It is a manufacturing method of the ultraviolet-proof base as described in any one of Claims 22-24 ,
A method for producing an ultraviolet-resistant base, wherein the concentration of the glycerin in the ultraviolet-proof base is adjusted to 4.5% by weight. It is a manufacturing method of the ultraviolet protection base according to claim 21 ,
A method for producing an anti-ultraviolet base, wherein hyaluronic acid is used as the viscous reagent. It is a manufacturing method of the ultraviolet-proof base of Claim 26 , Comprising:
A method for producing a UV-proof base, characterized in that the concentration of the disaggregated material in the mixture is adjusted to 0.036 to 0.96% in terms of weight concentration. It is a manufacturing method of the ultraviolet protection base according to claim 27 ,
A method for producing a UV-proof base, characterized in that the concentration of the disaggregated material in the mixture is adjusted to 0.36 to 0.40% in terms of weight concentration. It is a manufacturing method of the ultraviolet-proof base as described in any one of Claims 26-28 ,
A method for producing an anti-ultraviolet base comprising adjusting the concentration of the hyaluronic acid in the anti-ultraviolet base to 0.0005% in terms of weight concentration. It is a manufacturing method of a UV protection base according to any one of claims 21-29 ,
A method for producing an anti-ultraviolet base, wherein β1,4-glucan is used as the disaggregation product.