JP5505714B2 - Chemical treatment cartridge and method of using chemical treatment cartridge - Google Patents

Description

  The present invention relates to a chemical processing cartridge and a method of using the same, wherein at least a part is formed of an elastic body, and the chemical processing is performed by sealing or moving the contents by deformation caused by a force applied to the elastic body from the outside. .

  There is known a chemical processing cartridge in which at least a part is formed of an elastic body, and the chemical processing is performed by sealing or moving the contents by deformation caused by a force applied to the elastic body from the outside. In this chemical processing cartridge, wells and flow paths are formed in a shape and arrangement conforming to a desired chemical processing procedure, and by moving the contents by moving a roller or the like pressed against the cartridge, The chemical treatment according to the above procedure can be easily performed.

JP 2004-226068 A US Pat. No. 6,645,758

  However, there are cases where it is not easy to move the contents reliably, and in order to move the contents without waste and to perform a reliable chemical treatment, it is necessary to appropriately design wells and flow paths. For example, if the flow path for carrying the contents becomes long, the contents are likely to remain in the flow path, the contents are wasted, or stable chemical treatment cannot be performed (for example, US Pat. No. 6,645,758). No. Specification FIG. 1 etc.). In addition, a reliable mixed state cannot be created simply by injecting a plurality of contents into the same flow path.

  An object of the present invention is to provide a chemical processing cartridge and a method for using the chemical processing cartridge that can reliably mix contents without waste.

The cartridge for chemical processing according to the present invention is a cartridge for chemical processing which is at least partially formed of an elastic body and seals or moves the contents by deformation caused by a force applied to the elastic body from the outside to perform chemical processing. And a plurality of wells formed as spaces in the chemical treatment cartridge arranged in one direction and sequentially connected in series by a flow path, and a plurality of reagent holding parts respectively connected to the wells A reagent contained in each of the reagent holders is applied to each of the wells connected to the reagent holder by deformation of the chemical treatment cartridge by a force applied to the elastic body from the outside. A first step of moving the reagent, and the reagent contained in each of the wells by the first step from the outside of the cartridge By moving the attached member in the one direction, it is sequentially moved in the one direction through the flow path without flowing back to the reagent holding part and collected in one of the wells. The reagent is mixed by the second step .
According to this cartridge for chemical treatment, after the contents of the holding part are moved to the corresponding wells, the contents of the wells connected in series can be sequentially moved according to the connection order. Can be mixed reliably without waste.

In the second step, by pressing a member from the outside of the cartridge between the reagent holding part and the well connected to the reagent holding part, the reagent is prevented from flowing back to the reagent holding part. May be.

The method for using the chemical processing cartridge of the present invention is a chemistry in which at least a part is formed of an elastic body, and the contents are sealed or moved by deformation due to a force applied to the elastic body from the outside. In the method of using a processing cartridge, the chemical processing cartridge is arranged along one direction and sequentially connected in series by a flow path, and a plurality of wells formed as spaces in the chemical processing cartridge; A plurality of reagent holding parts connected to the wells, and the method for using the chemical processing cartridge is configured such that the reagents contained in the reagent holding parts are connected to the wells connected to the reagent holding parts. A first step of moving the elastic body by deformation of the chemical treatment cartridge by an external force applied to the elastic body; The reagent housed in each of the wells by the step is moved through the flow path without flowing back to the reagent holding part by moving a member pressed from the outside of the cartridge in the one direction. And a second step of mixing the reagents by sequentially moving them in one direction and collecting them in one of the wells .
According to this method of using the cartridge for chemical processing, after the contents of the holding part are moved to the corresponding wells, the contents of the wells connected in series are sequentially moved according to the connection order. Can be mixed reliably without waste.

  The flow path connecting the well and the holding portion by deforming the chemical processing cartridge by applying an external force to the chemical processing cartridge during the step of sequentially moving the contents of the well The step of closing may be provided.

  According to the cartridge for chemical processing of the present invention, the contents of the wells connected in series can be sequentially moved according to the connection order after the contents of the holding part are moved to the corresponding wells. You can mix things reliably without waste.

  According to the method of using the chemical processing cartridge of the present invention, after the contents of the holding part are moved to the corresponding wells, the contents of the wells connected in series are sequentially moved according to the connection order. You can mix things reliably without waste.

The top view which shows the structure of the cartridge for chemical processing of one Embodiment. It is sectional drawing of the cartridge for chemical processing shown in FIG. 1, (a) And (b) is the II sectional view taken on the line in FIG. The top view which shows arrangement | positioning of the roller for liquid feeding. The figure which shows the example which uses the roller for exclusive use which seals a flow path. The figure which shows the example which uses the shutter which seals a flow path.

  Hereinafter, an embodiment of the chemical processing cartridge according to the present invention will be described with reference to FIGS.

  FIG. 1 is a plan view showing the configuration of the chemical processing cartridge of the present embodiment, and FIGS. 2A and 2B are cross-sectional views taken along line II in FIG.

  As shown in FIGS. 1 and 2A, the chemical processing cartridge 100 of this embodiment includes a substrate 100A and a cover 100B made of an elastic body such as silicon rubber. The substrate 100A and the cover 100B are bonded to each other with a predetermined bonding pattern, and a well, a reagent holding portion, and a flow path, which will be described later, are formed as an internal space between the substrate 100A and the cover 100B in the non-bonded portion. This internal space is sealed and kept airtight.

  As shown in FIG. 1, the chemical processing cartridge 100 includes wells 1 to 6, reagent holding units 21 to 25, and flow paths that connect the wells 1 to 4 and the reagent holding units 21 to 25. Has been. The reagent holders 21 to 25 and the well 6 store a reagent or a cleaning solution.

  As shown in FIG. 1, the wells 1 to 4 are sequentially connected in series via the flow path, and the reagent holding unit 21 is located in the well 1, the reagent holding unit 22 is located in the well 2, and the reagent is held in the well 3. The part 23 is connected to the well 4 and the reagent holding part 24 via a flow path.

  In the region where the liquid exists in the internal space of the chemical processing cartridge 100, the cover 100B is elastically deformed by the internal pressure of the liquid, and the cover 100B is separated from the substrate 100A as shown in FIG. For example, FIG. 2A shows a case where liquid exists in the region of the well 24. In other regions, the cover 100B is in close contact with the substrate 100A, and the flow path 31 is sealed. When the liquid runs out, the cover 100B returns to its original shape, the cover 100B comes into contact with the substrate 100A, and the flow path is in a zero volume state. FIG. 2B shows a state in which liquid feeding is being performed. Since liquid exists in the well 6 and the flow path 31, the cover 100B is separated from the substrate 100A in that region.

  Next, a liquid feeding method in the chemical processing cartridge of this embodiment will be described.

  FIG. 3 is a plan view showing the arrangement of rollers for liquid feeding.

  As shown in FIG. 3, the rollers 41 to 56 are arranged at positions corresponding to the configuration of the chemical processing cartridge 100. In addition, about the liquid feeding using a some roller, Unexamined-Japanese-Patent No. 2006-26452 grade | etc., Discloses, The same structure is employable also in this invention.

  When the rollers 41 to 44 are pressed against the chemical processing cartridge 100 and simultaneously moved rightward in FIG. 3, the contents of the reagent holding unit 21 are transferred to the well 1 by the roller 41, and the contents of the reagent holding unit 22 are transferred to the roller 42. The contents of the reagent holding unit 23 are moved to the well 2 by the roller 43 and the contents of the reagent holding unit 24 are moved to the well 4 by the roller 44.

  Next, when the rollers 46 to 49 are pressed against the chemical processing cartridge 100 and simultaneously moved rightward by the movement width between the rollers in FIG. 3, the contents of the well 1 are transferred to the well 2 by the roller 46 and the well 47 is transferred to the well by the roller 47. The contents of 2 are moved to the well 3, the contents of the well 3 are moved to the well 4 by the roller 48, and the contents of the well 4 are moved to the well 5 by the roller 49. Further, when the rollers 46 to 49 are simultaneously moved rightward by the movement width between the rollers in FIG. 3, the contents of the well 2 are moved to the well 3 by the roller 46, and the contents of the well 3 are moved to the well 4 by the roller 47. The roller 48 moves the contents of the well 4 to the well 5 respectively. Further, when the rollers 46 to 49 are simultaneously moved rightward by the movement width between the rollers in FIG. 3, the contents of the well 3 are transferred to the well 4 by the rollers 46, and the contents of the well 4 are transferred to the well 5 by the rollers 47. Move each one. Further, when the rollers 46 to 49 are simultaneously moved rightward by the movement width between the rollers in FIG. 3, the contents of the well 4 are moved to the well 5 by the roller 46.

  As described above, the contents of the reagent holders 21 to 24 are mixed in the well 5 via the wells 1 to 4 by the movement of the rollers 46 to 49.

  Further, during this time, when the rollers 55 to 56 are pressed against the chemical processing cartridge 100 and simultaneously moved rightward by the movement width between the rollers in FIG. 3, the contents of the well 6 move to the well 5, and the reagent holders 21 to 21 are moved. 24 contents are mixed in well 5.

  Next, when the rollers 51 to 54 are pressed against the chemical processing cartridge 100 and moved simultaneously in the right direction in FIG. 3, the contents of the well 5 are moved to the next well (not shown) by the roller 54.

  As described above, according to the chemical processing cartridge of the present embodiment, the wells 1 to 4 corresponding to the reagent holding units 21 to 24 are provided, and the distance from the corresponding reagent holding unit to the well can be shortened. The contents of the reagent holding part can be transported to the well without waste, and the reagent and solution can be used effectively. In addition, since the reagent holding portions 21 to 24 are arranged on the outer edge portion of the cartridge 100, the arrangement of the flow paths and wells that define the processing and reaction steps is not obstructed, and the degree of freedom in arrangement can be increased.

  In addition, since the step of moving the contents of the reagent holders 21 to 24 to the corresponding wells 1 to 4 and the step of mixing them are separated, reliable and accurate liquid feeding becomes possible, Can move and mix reliably.

  Furthermore, since rollers are arranged at positions corresponding to the individual reagent holding units 21 to 24 and the wells 1 to 4, respectively, and liquid feeding is performed step by step, individual liquid feeding can be controlled independently. Accurate liquid feeding in line with this process is guaranteed. Further, by maintaining the state where the roller is pressed against the cartridge, it is possible to cause the roller to function as a shutter, so that it is possible to effectively prevent backflow of contents and unintentional liquid feeding to the previous well.

  In addition, by repeating the movement of the roller, it is possible to cope with a case where liquid feeding is incomplete, and the reliability of liquid feeding can be improved.

  FIG. 4 shows an example in which a dedicated roller for sealing the flow path is used.

  In FIG. 4, a roller 61 is a roller for sealing the flow path between the reagent holding units 21 to 24 and the wells 1 to 4, and has a function of preventing the backflow of contents to the well 1. When the liquid is fed by the roller 46, the flow path is blocked by the roller 61, thereby preventing the backflow to the reagent holding units 21 to 24.

  In the example of FIG. 4, the contents of the wells 21 to 24 are respectively introduced into the wells 1 to 4 by moving one roller 41 in the right direction. Thereafter, the contents are sequentially moved toward the well 5 by moving the roller 46 and the roller 61 in the right direction. At this time, backflow to the reagent holding parts 21 to 24 is prevented by the roller 61.

  A shutter that is pressed against the cartridge may be used instead of the roller 61, and the functions of the roller 46 and the roller 61 may be combined with an L-shaped squeegee.

  FIG. 5 shows an example in which a shutter for sealing the flow path is used.

  In FIG. 5, the shutter 62 seals the flow path between the reagent holding units 21 to 24 and the wells 1 to 4.

  In the example of FIG. 5, the contents of the wells 21 to 24 are respectively introduced into the wells 1 to 4 by moving one roller 41 in the right direction with the shutter 62 opened. Thereafter, the roller 46 is moved in the right direction with the flow path closed by the shutter 62, whereby the contents are sequentially moved toward the well 5. At this time, backflow to the reagent holding parts 21 to 24 is prevented by the shutter 62.

Note that the same effect may be obtained by using a short shutter in the left-right direction in FIG. 5 and moving the shutter in the right direction in conjunction with the roller 46 in a state where the shutter is pressed against the cartridge.
Further, the reagent holding part may be installed on the outermost peripheral part of the cartridge. This facilitates introduction of the reagent.

  As described above, according to the chemical processing cartridge of the present invention, after the contents of the holding portion are moved to the corresponding wells, the contents of the wells connected in series are sequentially moved according to the connection order. Therefore, the contents can be reliably mixed without waste.

  The scope of application of the present invention is not limited to the above embodiment. The present invention can be widely applied to a chemical processing cartridge for performing chemical processing by moving contents by deformation caused by externally applied force and a method for using the same.

1-6 well 21-24 reagent holding part (holding part)
25 Reagent holding part (holding part)

Claims (4)

In the chemical treatment cartridge, wherein at least a part is formed of an elastic body, and the chemical treatment is performed by sealing or moving the contents by deformation due to a force applied to the elastic body from the outside.
A plurality of wells formed as spaces in the chemical treatment cartridge, arranged in one direction and sequentially connected in series by flow paths;
A plurality of reagent holding portions respectively connected to the wells;
With
A reagent stored in each reagent holding part is moved to each of the wells connected to the reagent holding part by deformation of the chemical treatment cartridge by a force externally applied to the elastic body. Steps,
The flow path without causing the reagent stored in each of the wells in the first step to flow back to the reagent holding portion by moving a member pressed from the outside of the cartridge in the one direction. A second step that sequentially moves in the one direction through and collects in one of the wells;
A cartridge for chemical processing, wherein the reagent is mixed by the method described above . In the second step, by pressing a member from the outside of the cartridge between the reagent holding part and the well connected to the reagent holding part, the reagent is prevented from flowing back to the reagent holding part. chemical reaction cartridge according to claim 1, characterized in that that. In a method of using a chemical treatment cartridge, wherein at least a part is formed of an elastic body, and the chemical treatment is performed by sealing or moving the contents by deformation due to a force applied to the elastic body from the outside.
The chemical processing cartridge is:
A plurality of wells formed as spaces in the chemical treatment cartridge, arranged in one direction and sequentially connected in series by flow paths;
A plurality of reagent holding portions respectively connected to the wells;
With
How to use chemical processing cartridges
A reagent stored in each reagent holding part is moved to each of the wells connected to the reagent holding part by deformation of the chemical treatment cartridge by a force externally applied to the elastic body. Steps,
The flow path without causing the reagent stored in each of the wells in the first step to flow back to the reagent holding portion by moving a member pressed from the outside of the cartridge in the one direction. A second step of mixing the reagents by sequentially moving them in the one direction through and collecting them in one of the wells;
A method for using a cartridge for chemical processing, comprising: In the second step, by pressing a member from the outside of the cartridge between the reagent holding part and the well connected to the reagent holding part, the reagent is prevented from flowing back to the reagent holding part. the method of using a chemical reaction cartridge according to claim 3, characterized in that that.

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