JP5238946B1 - Biological treatment agent - Google Patents

Abstract

A novel biological treatment agent that can be used for wastewater treatment by an activated sludge method containing wastewater containing organic compounds, or for environmental purification by bioremediation of soil contaminated with organic compounds, groundwater, etc. A wastewater treatment method or an environmental purification method using a biological treatment agent is provided.
A biological treatment agent containing water, hexametaphosphate, glutathione oxidized form, glycerol dehydrogenase, yeast lytic enzyme, glycerin, peroxodisulfate, and ethylenediaminetetraacetic acid, and A wastewater treatment method using an activated sludge method containing wastewater containing an organic compound or the like using a biological treatment agent, or an environmental purification method using bioremediation of soil, groundwater, etc. contaminated with an organic compound or the like.
[Selection figure] None

Description

  The present invention relates to a biological treatment agent. More specifically, the present invention relates to a biological treatment that can be used for treatment of wastewater containing organic compounds by the activated sludge method and / or purification by bioremediation of soil contaminated with organic compounds or the like, groundwater, etc. It relates to the agent.

  As a technique for removing organic substances from wastewater containing organic compounds such as industrial wastewater and sewage, the activated sludge method is widely used because of its advantages such as good quality of treated water and easy maintenance. However, the activated sludge method has a problem that a large amount of activated sludge is generated with the treatment, and the cost for the disposal is high. Therefore, the reduction of surplus sludge generation has become an important theme for reducing costs in the activated sludge method.

  For example, Non-Patent Document 1 describes a technique in which a microorganism cell in excess sludge is destroyed by using an inorganic oxygen-based oxidizing agent to solubilize the microorganism.

  The sludge reduction equipment using this technology is a large-scale equipment that has a sludge concentration tank, sludge storage tank, chemical reaction tank, chemical storage tank, etc., and a part of the excess sludge that has been separated from the treated water in the final sedimentation tank. It is sent to the sludge concentration tank, the excess sludge is concentrated in the sludge concentration tank, the concentrated sludge is temporarily stored in the sludge storage tank, the concentrated sludge is solubilized with the oxidizing agent in the chemical reaction tank, and the chemical reaction is performed in the chemical storage tank. The chemical | medical agent supplied to a tank is stored, and the sludge solubilized in the chemical | medical agent reaction tank is returned to a biological reaction tank (aeration tank). However, since the facilities are large, it is difficult to say that they can be easily introduced into existing wastewater treatment facilities.

  On the other hand, bioremediation is well known as a technique for purifying environmental pollution by decomposing and absorbing pollutants using the action of microorganisms and the like. Biological methods such as physical methods, chemical methods, and the use of microbial functions exist as technical methods for environmental pollution purification, but bioremediation using microorganisms has the potential to be applied to various pollutants. It is considered that it is one of the main technologies in the future because it has theoretically low input energy and may generally require low purification costs.

  Bioremediation is classified into two types based on the utilization of microorganisms. One is called biostimulation, and organic matter as an energy source necessary for the growth of microorganisms such as inorganic nutrients such as nitrogen and phosphorus, methane, compost, etc. in the contaminated soil and sediment, and air. This is a method to increase the purification activity by introducing microorganisms and hydrogen peroxide, etc., and multiplying the microorganisms that live on the site. The other is called bioaugmentation, where purified microorganisms inhabit the contaminated site. If not, it is a method of introducing and purifying microorganisms cultured elsewhere. When the contaminated environment is compared to a sick person, biostimulation corresponds to nutrition and physical strength enhancement, and bioaugmentation corresponds to treatment using medication when symptoms are severe.

  The bioremediation method is considered to be advantageous in terms of cost compared to other remediation methods, and in particular, is attracting attention as a purification technique for soil, groundwater, and the like contaminated with organic compounds. However, since bioremediation uses the action of microorganisms, it takes a long time to purify.

Takashi Ishida, “Overview of Technology Manual for Reduction of Surplus Sludge Using Oxidizing Agent”, [online], 2008, Sewerage New Technology Promotion Organization, [Search July 13, 2011], Internet <URL: http://www.jiwet.jp/result/technical_manual/pdf/2008_08.pdf>

  Accordingly, the present invention provides a novel biological treatment agent that can be used for treatment by wastewater containing organic compounds by the activated sludge method and / or purification by bioremediation of soil, groundwater, etc. contaminated with organic compounds. And further, a method for treating wastewater containing organic compounds by an activated sludge method using this biological treatment agent, or a method for purification by bioremediation of soil contaminated with organic compounds, groundwater, etc. Is an issue.

The present inventor has conducted extensive studies to solve the above-mentioned problems. As a result, hexametaphosphate, glutathione-oxidized form, glycerol dehydrogenase, yeast lytic enzyme, glycerin, peroxodisulfate, and ethylenediaminetetrachloride. An aqueous composition containing acetic acid and water is a novel biological treatment agent, and the treatment method using an activated sludge method for wastewater containing organic compounds using this biological treatment agent, and contamination with organic compounds, etc. As a result, it was found that a purification method by bioremediation of soil, groundwater, etc. can be provided, and the present invention has been completed.
That is, the present invention provides the following (1) to (14).

(1) 4000 parts by mass of water, 800 to 1600 parts by mass of sodium hexametaphosphate, 0.001 to 0.100 parts by mass of glutathione oxidation type, and 0.001 to 0.100 parts by mass of 50 U / mg glycerol dehydrogenase Glycerol dehydrogenase corresponding to the number of units is mixed, and further incubated for 5 days or more to prepare a mixed solution (A), 5200 parts by mass of the prepared mixed solution (A) and 5000 U / g yeast lytic enzyme A unit number of yeast lytic enzyme corresponding to 50 to 150 parts by mass and 800 to 1500 parts by mass of glycerin are mixed , further incubated for 3 days or more to prepare a mixed solution (B), and the prepared mixed solution (B) It corresponds to 800 parts by mass, 1000 to 4000 parts by mass of sodium peroxodisulfate, and 10 to 200 parts by mass of ethylenediaminetetraacetic acid. Molar number of ethylenediaminetetraacetic acid or a water-soluble salt thereof is mixed with the mixture (B), sodium peroxodisulfate and water in an amount capable of dissolving the ethylenediaminetetraacetic acid or the water-soluble salt thereof, and further incubated for 5 days or more. A biological treatment agent obtained by preparing a mixed solution (C) and diluting the prepared mixed solution (C) as it is or with water .

(2) 4000 parts by mass of water, 800 to 1600 parts by mass of sodium hexametaphosphate, 0.001 to 0.100 parts by mass of glutathione oxidation type, and 0.001 to 0.100 parts by mass of 50 U / mg glycerol dehydrogenase preparing a mixture of a number of units of glycerol dehydrogenase corresponding further 5 days or more incubated with mixture (a), the
5200 parts by mass of the prepared mixed liquid (A) , yeast lytic enzyme having a unit number corresponding to 50 to 150 parts by mass of 5000 U / g yeast lytic enzyme, and 800 to 1500 parts by mass of glycerin are mixed, and further 3 days or more Incubating to prepare a mixture (B); and
And prepared mixture liquid (B) 800 parts by weight, and Bae Ruokiso disodium sulphate 1000-4000 parts by weight, and the number of moles of ethylene diamine tetraacetic acid or its water-soluble salts corresponding to ethylenediaminetetraacetic acid 10 to 200 parts by weight, the mixing liquid (B), comprising the step of the peroxodisulfate water in an amount mixed capable sodium sulfate and dissolve the ethylenediaminetetraacetic acid or its water-soluble salts, to prepare further 5 days or more incubated with mixture (C), live A method for producing a material treatment agent.

(3) A wastewater treatment method by an activated sludge method for wastewater containing an organic compound and / or a BOD component,
A wastewater treatment method comprising: a mixing step of mixing the wastewater, activated sludge, and the biological treatment agent according to (1) above; and an aeration step of aerating the mixed solution obtained in the mixing step.
(4) The wastewater treatment method according to (3), wherein in the mixing step, the biological treatment agent is added to a mixed solution containing the wastewater and the activated sludge.
(5) The wastewater treatment method according to (3), wherein, in the mixing step, the biological treatment agent is added to a mixed solution containing the wastewater and the activated sludge together with the return sludge.
(6) When adding the biological treatment agent, the amount of dissolved oxygen in the mixed solution is measured, and the addition amount of the biological treatment agent is adjusted based on the amount of dissolved oxygen, (4) or (5) The wastewater treatment method described in 1.
(7) The above wastewater is selected from the group consisting of food processing wastewater, beverage manufacturing wastewater, fermentation / brewing wastewater, pharmaceutical industry wastewater, chemical industry wastewater, textile industry wastewater, scouring / dyeing wastewater, fat and oil industry wastewater, sewage and settlement wastewater. The wastewater treatment method according to any one of (3) to (6), wherein the wastewater treatment method is at least one.
(8) A wastewater treatment facility for performing wastewater treatment by an activated sludge method containing wastewater containing organic compounds and / or BOD components,
An aeration tank for aeration of a mixed liquid containing the wastewater, activated sludge, and the biological treatment agent according to (1),
A chemical reservoir for storing the biological treatment agent;
An input device for supplying the biological treatment agent from the drug storage tank to the aeration tank;
Wastewater treatment facility comprising.
(9) A wastewater treatment facility for treating wastewater by an activated sludge method containing wastewater containing organic compounds and / or BOD components,
An aeration tank for aeration of a mixed liquid containing the wastewater, activated sludge, and the biological treatment agent according to (1),
A settling tank for precipitating and separating activated sludge from the treated water flowing out of the aeration tank or a separation membrane for separating activated sludge from the treated water flowing out of the aeration tank;
A return sludge line for returning the activated sludge extracted from the settling tank or the activated sludge separated by the separation membrane to the aeration tank;
A chemical reservoir for storing the biological treatment agent;
An input device for supplying the biological treatment agent from the chemical storage tank to the return sludge line;
Wastewater treatment facility comprising.
(10) The above wastewater is selected from the group consisting of food processing wastewater, beverage manufacturing wastewater, fermentation / brewing wastewater, pharmaceutical industry wastewater, chemical industry wastewater, textile industry wastewater, scouring / dyeing wastewater, fat and oil industry wastewater, sewage and settlement wastewater. The wastewater treatment facility according to (8) or (9), wherein the wastewater treatment facility is at least one.

(11) A method for environmental purification by bioremediation of soil, sediment, groundwater, river water, lake water or seawater contaminated with organic compounds and / or BOD components,
An environmental purification method comprising a step of adding and mixing the biological treatment agent according to (1) above to the soil, sediment, groundwater, river water, lake water, or seawater mixed with a bioremediation nutrient.
(12) A method for environmental purification by bioremediation of soil, sediment, groundwater, river water, lake water or seawater contaminated with organic compounds and / or BOD components,
The biological treatment agent according to (1) above, wherein the soil, bottom sediment, groundwater, river water, lake water, or seawater mixed with the microorganism having the ability to remove the organic compound and / or BOD component and the bioremediation nutrient are mixed. An environmental purification method comprising the steps of adding and mixing.
(13) The environmental purification method according to (11) or (12), wherein the organic compound and / or BOD component is a volatile organic compound (VOC).
(14) The volatile organic compound is benzene, 1,1-dichloroethylene, dichloromethane, cis-1,2-dichloroethylene, 1,1,1-trichloroethane, carbon tetrachloride, 1,2-dichloroethane, trichloroethylene, 1, The environmental purification method according to (13), which is at least one selected from the group consisting of 1,2-trichloroethane, 1,3-dichloropropane and tetrachloroethylene.

  ADVANTAGE OF THE INVENTION According to this invention, the novel biological treatment agent which can be used for the wastewater treatment by the activated sludge method of the wastewater containing an organic compound etc. and / or the purification process by bioremediation of the soil contaminated with the organic compound, groundwater, etc. Can be provided.

  Furthermore, this invention can provide the wastewater treatment method by the activated sludge method of the wastewater containing an organic compound etc. which uses this biological treatment agent. This wastewater treatment method can be easily introduced even in existing wastewater treatment facilities.

  According to this treatment method, the amount of activated sludge suspended matter (MLSS) generated can be reduced, so the amount of surplus sludge can be reduced, and the cost and energy consumption for disposing of surplus sludge can be suppressed. be able to. Further, in the membrane separation activated sludge method, clogging of the separation membrane can be reduced, so that the replacement frequency of the separation membrane can be reduced and it is useful for cost reduction.

  In addition, according to this treatment method, the amount of activated sludge suspended matter (MLSS) can be reduced, and the dissolved oxygen amount (DO) can be increased. The activity of microorganisms is maintained, and wastewater treatment efficiency can be increased.

  Furthermore, the present invention provides a method for environmental purification using bioremediation (either biostimulation or bioaugmentation) of soil contaminated with organic compounds, groundwater, etc., using this biological treatment agent. Can do. According to this environmental purification method, it is possible to shorten the time required for environmental purification by decomposing the pollutant.

[Biological treatment agent and method for producing the same]
The biological treatment agent of the present invention is a biological treatment containing water, hexametaphosphate, glutathione oxidation type, glycerol dehydrogenase, yeast lytic enzyme, glycerin, peroxodisulfate, and ethylenediaminetetraacetic acid. It is an agent.

  The biological treatment agent comprises a step of mixing water, hexametaphosphate, glutathione oxidized form, and glycerol dehydrogenase to prepare a mixed solution (A), the mixed solution (A), a yeast lytic enzyme, , Mixing glycerin to prepare a mixed solution (B), and mixing the mixed solution (B), water, peroxodisulfate, and ethylenediaminetetraacetic acid to prepare a mixed solution (C) It can manufacture with the manufacturing method including the process to carry out.

  The mixed liquid (C) may be used as it is as the biological treatment agent of the present invention, or may be diluted with water or other diluent and used as the biological treatment agent of the present invention.

Hereinafter, the biological treatment agent and the production method thereof of the present invention will be described in detail.
<Mixed liquid (A)>
The mixed solution (A) can be prepared by mixing water, hexametaphosphate, glutathione oxidation type, and glycerol dehydrogenase. If desired, further incubation may be performed.

  Although it is desirable to perform the step of preparing the mixed solution (B) immediately after the preparation of the mixed solution (A), when the step of preparing the mixed solution (B) is not performed immediately, the prepared mixed solution (A ) May be stored at 0 to 5 ° C. avoiding ultraviolet rays.

"water"
The water is not particularly limited, but preferably has an electrical resistivity of 1 MΩ · cm (25 ° C.) or more, that is, an electrical conductivity of 1 μS / cm (25 ° C.) or less, and an electrical resistivity of 10 MΩ · cm (25 ° C.) or more. That is, it is more preferable that the electric conductivity is 0.1 μS / cm (25 ° C.) or less.

  As said water, the water of JISK0557: 1998 A2-A4, such as Japanese Pharmacopoeia purified water, sterilized purified water, water for injection etc., can be used, for example, what is called industrial purified water can also be used. In addition, as the water, for example, tap water, well water, ground water or the like can be used as it is, or purified water obtained by purifying them by a technique such as distillation, filtration and / or ion exchange.

《Hexametaphosphate》
The hexametaphosphate is not particularly limited as long as it is water-soluble, but a water-soluble metal salt is preferable, an alkali metal salt is more preferable, a sodium salt or a potassium salt is further preferable, and a sodium salt is more preferable.

When sodium hexametaphosphate is used as the hexametaphosphate, the sodium hexametaphosphate is not limited to sodium hexametaphosphate (CAS # 10124-56-8) represented by the composition formula (NaPO ₃ ) ₆ , but the chemical formula (NaPO ₃₎ may be a mixture of those represented by _n (preferably n = 5.8 to 6.2). The grade is not particularly limited, and various grades for industrial use, food use, reagent use and the like can be used.

  The blending amount of the hexametaphosphate is not particularly limited, but when sodium hexametaphosphate is used as the hexametaphosphate, it is preferably 800 with respect to 4000 parts by mass of the water in the mixed solution (A). -1600 mass parts, More preferably, it is 900-1500 mass parts, More preferably, it is 1000-1400 mass parts, More preferably, it is 1100-1300 mass parts.

  When hexametaphosphate other than sodium hexametaphosphate is used as the hexametaphosphate, it is desirable to adjust the blending amount based on the molecular weight so that the number of moles is about the same.

<Glutathione oxidation type>
The glutathione oxidation type is not particularly limited, and CAS # 27025-41-8 can be used.

  The blending amount of the glutathione oxidation type is not particularly limited, but is preferably 0.001 to 0.100 parts by mass, more preferably 0.005 with respect to 4000 parts by mass of the water in the mixed solution (A). -0.050 mass part, More preferably, it is 0.010-0.040 mass part, More preferably, it is 0.015-0.035 mass part.

<Glycerol dehydrogenase>
The glycerol dehydrogenase (glycerol: NAD ⁺ 2-oxidoreductase) is not particularly limited, and those of EC 1.1.1.6 can be used.

  The amount of the glycerol dehydrogenase to be blended is not particularly limited, but when the glycerol dehydrogenase having an enzyme activity of 50 U / mg per unit mass is used in the mixture (A), Preferably it is 0.001-0.100 mass part with respect to 4000 mass parts of water, More preferably, it is 0.005-0.050 mass part, More preferably, it is 0.005-0.030 mass part, More preferably, it is 0. 0.005 to 0.020 parts by mass.

  When the glycerol dehydrogenase having different enzyme activity (U / mg) per unit mass is used, it is desirable to adjust the blending amount (mg) so that the enzyme activity is almost the same as the whole. .

"mixture"
Each of the above components may be mixed with other components in two or more times.
The above-mentioned components can be mixed using a glass container such as a beaker or an Erlenmeyer flask, a plastic container such as a polybin, or other conventionally known containers.
The temperature during mixing of the above components is not particularly limited, and may be room temperature (for example, 20 ± 15 ° C.), but is preferably 15 ° C. or less, and more preferably 0 to 10 ° C.

Incubation
In the case of further incubation, the incubation temperature is not particularly limited, and may be a working environment temperature or room temperature (for example, 20 ± 15 ° C.), but is preferably 0 to 8 ° C., more preferably 0 to 5 ° C. The temperature can be adjusted by a conventionally known method, but it is desirable to use an incubator with a temperature adjusting function, a thermostatic bath, a refrigerator or the like.

  The incubation time is not particularly limited, and may be a short time that inevitably occurs after mixing and before proceeding to the next step, but is preferably 5 days or more, more preferably 7 days or more, 7 10 days is more preferable.

  Other conditions for the incubation are not particularly limited, but it is preferable to perform in the atmosphere, avoid direct sunlight and ultraviolet rays, leave the mixture without stirring. When incubating, it is desirable to incubate in the dark. Incubating in the dark improves the stability of the mixture and shortens the incubation time to approximately half.

<Mixed liquid (B)>
The mixed solution (B) can be prepared by mixing the mixed solution (A), a yeast lytic enzyme, and glycerin. If desired, further incubation may be performed.

  It is desirable to perform the step of preparing the mixed solution (C) immediately after the preparation of the mixed solution (B). However, when the step of preparing the mixed solution (C) is not performed immediately, avoid ultraviolet rays and avoid 0 to 0. You may preserve | save at 10 degreeC.

《Yeast lytic enzyme》
The yeast lytic enzyme is a β-1,3-glucanase (EC 3.2.) That hydrolyzes the (1 → 3) -β-D-glucoside bond of (1 → 3) -β-D-glucan in the yeast cell wall. 1.39) Any yeast cell wall lytic activity such as activity can be used without particular limitation.

  The blending amount of the yeast lytic enzyme is not particularly limited. When the yeast lytic enzyme having an enzyme activity per unit mass of 5000 U / g is used, the mixed liquid (B) is mixed with the mixed liquid. (A) With respect to 5200 mass parts, Preferably it is 50-150 mass parts, More preferably, it is 70-130 mass parts, More preferably, it is 70-120 mass parts, More preferably, it is 80-110 mass parts.

  When using yeast lytic enzymes having different enzyme activities per unit mass (U / g), it is desirable to adjust the blending amount (g) so that the enzyme activities are substantially the same.

  Specific examples of the yeast lytic enzyme include yeast lytic enzyme (manufactured by Kanto Chemical Co., Ltd., 5000 U / g), Zymolase-20T (manufactured by MP Biomedicals Co., Ltd., 20000 U / g), Westase (manufactured by Takara Bio Inc.). 35000 U / g) or the like.

《Glycerin》
The glycerin is not particularly limited, and CAS # 56-81-5 can be used. Moreover, the said glycerin is not limited to a reagent grade thing, Industrial use, the foodstuff (addition) use, etc. can be used.

  Although the compounding quantity of the said glycerol is not specifically limited, Preferably it is 800-1500 mass parts with respect to 5200 mass parts of the said mixed liquid (A) in the said mixed liquid (B), More preferably, it is 900-1200 mass parts. Is more preferably 950 to 1150 parts by mass.

"mixture"
Each component may be mixed with other components in two or more times.
Mixing of each component can be performed using glass containers, such as a beaker and an Erlenmeyer flask, plastic containers, such as a polybin, and other conventionally well-known containers.
Although the temperature during mixing of each component is not specifically limited, Room temperature (for example, 20 ± 15 degreeC) may be sufficient, and low temperature of 0-10 degreeC or 0-5 degreeC may be sufficient.

Incubation
In the case of further incubation, the incubation temperature is not particularly limited, and may be a working environment temperature or room temperature (for example, 20 ± 15 ° C.), preferably 35 to 42 ° C., and more preferably 37 to 40 ° C. The temperature can be adjusted by a conventionally known method, but it is desirable to use an incubator with a temperature adjusting function, a thermostatic bath, a refrigerator or the like.

  The incubation time is not particularly limited, and may be a short time that inevitably occurs after mixing and before proceeding to the next step, but is preferably 3 days or more, more preferably 5 days or more. More preferred is ~ 7 days.

  Other conditions for the incubation are not particularly limited, but it is preferable to perform in the atmosphere, avoid direct sunlight and ultraviolet rays, leave the mixture without stirring.

<Mixed liquid (C)>
The mixed liquid (C) can be prepared by mixing the mixed liquid (B), peroxodisulfate, ethylenediaminetetraacetic acid, and water. If desired, further incubation may be performed.

  The above mixed solution (C) can be used as the biological treatment agent of the present invention as it is, or diluted with water. Moreover, the biological treatment agent of the present invention can be further diluted for use.

  When diluting the mixed liquid (C) with water, it is desirable to dilute immediately after the preparation of the mixed liquid (C). It is desirable to store at 20 ± 15 ° C.

  When the above mixed solution (C) is used as a raw solution as the biological treatment agent of the present invention, it is desirable to use it immediately, but it may be stored at room temperature (20 ± 15 ° C.) avoiding ultraviolet rays.

  When the mixed solution (C) is diluted and used as the biological treatment agent of the present invention, it is desirable to use it immediately, but it may be stored at room temperature (20 ± 15 ° C.) avoiding ultraviolet rays.

《Peroxodisulfate》
The peroxodisulfate is not particularly limited as long as it is a water-soluble salt, but a water-soluble metal salt is preferable, an alkali metal salt is more preferable, a sodium salt or a potassium salt is further preferable, and a sodium salt is more preferable.

  When sodium peroxodisulfate is used as the peroxodisulfate, the sodium peroxodisulfate is preferably CAS # 7775-27-1. The grade of sodium peroxodisulfate is not particularly limited, and various grades can be used.

  When sodium peroxodisulfate is used as the peroxodisulfate, the blending amount thereof is not particularly limited, but is preferably 1000 with respect to 800 parts by mass of the mixed liquid (B) in the mixed liquid (C). -4000 mass parts, More preferably, it is 1500-3500 mass parts, More preferably, it is 1750-3250 mass parts, More preferably, it is 2000-3000 mass parts.

  When the water-soluble metal salt other than sodium peroxodisulfate such as potassium peroxodisulfate is used as the peroxodisulfate instead of sodium peroxodisulfate, it is desirable to adjust the blending amount based on the molecular weight.

《Ethylenediaminetetraacetic acid》
The ethylenediaminetetraacetic acid is not particularly limited, and those of CAS # 60-00-4 can be used.

  Although the compounding quantity of the said ethylenediaminetetraacetic acid is not specifically limited, Preferably it is 10-200 mass parts with respect to 800 mass parts of the said mixed liquid (B) in the said mixed liquid (C), More preferably, it is 30-150. It is 50 mass parts, More preferably, it is 50-130 mass parts, More preferably, it is 80-120 mass parts.

  As the ethylenediaminetetraacetic acid, a water-soluble salt of ethylenediaminetetraacetic acid may be used instead of ethylenediaminetetraacetic acid. Specifically, for example, ethylenediaminetetraacetic acid disodium dihydrogen dihydrate, ethylenediaminetetraacetic acid tetraacetate, and the like. Metal salts (including hydrates) such as sodium dihydrate and disodium magnesium ethylenediaminetetraacetate hydrate can be used.

  When a water-soluble salt of ethylenediaminetetraacetic acid is used as the ethylenediaminetetraacetic acid, it is desirable to adjust the blending amount based on the molecular weight so that the total number of moles is about the same.

"water"
The water is not particularly limited, but preferably has an electrical resistivity of 1 MΩ · cm (25 ° C.) or more, that is, an electrical conductivity of 1 μS / cm (25 ° C.) or less, and an electrical resistivity of 10 MΩ · cm (25 ° C.) or more. That is, it is more preferable that the electric conductivity is 0.1 μS / cm (25 ° C.) or less.

  As said water, the water of JISK0557: 1998 A2-A4, such as Japanese Pharmacopoeia purified water, sterilized purified water, water for injection etc., can be used, for example, what is called industrial purified water can also be used. In addition, as the water, for example, tap water, well water, ground water or the like can be used as it is, or purified water obtained by purifying them by a technique such as distillation, filtration and / or ion exchange.

  The blending amount of the water is not particularly limited as long as it is an amount capable of dissolving the mixed solution (B), the peroxodisulfate, and the ethylenediaminetetraacetic acid. The total amount of the mixed liquid (C) is not particularly limited as long as the solute does not precipitate. However, the amount of the mixed liquid (C) is preferably 10,000 to 100,000 parts by mass, more preferably 10,000 with respect to 800 parts by mass of the mixed liquid (B). It is the quantity used as -40000 mass parts, More preferably, it is the quantity used as 10000-20000 mass parts.

"mixture"
Each component may be mixed with other components in two or more times.
Mixing of each component can be performed using glass containers, such as a beaker and an Erlenmeyer flask, plastic containers, such as a polybin, and other conventionally well-known containers.
Although the temperature during mixing each component is not specifically limited, Room temperature (for example, 20 ± 15 degreeC) may be sufficient, and low temperature of 0-10 degreeC or 0-5 degreeC may be sufficient.

Incubation
In the case of incubation, the incubation temperature is not particularly limited, but is preferably a working environment temperature or room temperature (for example, 20 ± 15 ° C.). Although the temperature may not be adjusted, it may be performed by a conventionally known method, and it is desirable to use an incubator with a temperature adjusting function, a thermostatic bath, a refrigerator, or the like.

  The incubation time is not particularly limited, and may be after mixing until use, but is preferably 5 days or more, more preferably 7 days or more, and further preferably 7 to 14 days.

  Other conditions for the incubation are not particularly limited, but it is preferable to perform in the atmosphere, avoid direct sunlight and ultraviolet rays, leave the mixture without stirring. When incubating, it is desirable to incubate in the dark. Incubating in the dark improves the stability of the mixture and shortens the incubation time to approximately half.

  In this specification, 1200 g of sodium hexametaphosphate, 20 mg of glutathione oxidized form, and 10 mg of glycerol dehydrogenase are added to 4000 g of purified water, and the mixture is stirred and mixed. Further, 0 to 5 ° C., normal pressure (approximately 1013 hPa) ) For 7 days to prepare a mixed solution (A). To 5200 g of the mixed solution (A), 100 g of yeast-dissolving enzyme and 1020 g of glycerin are added, and stirred and mixed. A mixed solution (B) was prepared by incubating at 5 ° C. and normal pressure (approximately 1013 hPa) for 5 days. To 10000 g of purified water, 800 g of the mixed solution (B), 2500 g of sodium peroxodisulfate, and 100 g of ethylenediaminetetraacetic acid were prepared. Are added and stirred and mixed, and ultraviolet rays are applied at room temperature (approximately 20 ° C.) and normal pressure (approximately 1013 hPa). Only, mixture (C) was prepared by incubating the biological treatment agent produced the mixture total amount of (C) as 20000 parts by mass of water, in particular, it may be referred to as "standard biological treatment agent".

[Wastewater treatment method]
The wastewater treatment method of the present invention is a wastewater treatment method by an activated sludge method of wastewater containing an organic compound or the like, and a mixing step of mixing the wastewater, the activated sludge, and the biological treatment agent of the present invention, and The aeration process of aerating the mixed liquid obtained in the mixing process is provided.

  In the wastewater treatment method and wastewater treatment facility of the present invention, “organic compound and the like” means “organic compound and / or BOD component”.

  The organic compound is a compound having a carbon atom in the basic skeleton of the structure (carbon allotrope such as graphite and diamond; carbon monoxide, carbon dioxide; metal carbonate such as calcium carbonate; cyanic acid, metal cyanate and metal cyanate. And cyan compounds such as metal thiocyanates; other inorganic compounds having carbon atoms are excluded.)

  Specific examples of the organic compound include parathion, methyl parathion, methyl dimethone, EPN and other organic phosphorus compounds, polychlorinated biphenyl (PCB), dichloromethane, carbon tetrachloride, trichloroethylene, tetrachloroethylene, 1,2-dichloroethane, 1 1,1-dichloroethylene, cis-1,2-dichloroethylene, 1,1,1-trichloroethane, 1,1,2-trichloroethane, 1,3-dichloropropene, thiuram, simazine, thiobencarb, benzene, phenols, etc. .

  The BOD component is not particularly limited as long as it is measured as a BOD component by the method defined in Item 21 of JIS K 0102: 2008, and may be either an organic BOD component or an inorganic BOD component.

  Specific examples of the organic BOD component include saccharides, organic acids, starches, proteins, and lipids.

  Specific examples of the inorganic BOD component include sulfides, sulfite ions, cyanide compounds, and other reducing substances.

  Wastewater containing the above organic compounds is not particularly limited, but is not limited to food processing wastewater, beverage manufacturing wastewater, fermentation / brewing wastewater, pharmaceutical industry wastewater, chemical industry wastewater, textile industry wastewater, scouring / dyeing wastewater, fat and oil industry wastewater, sewage And at least one selected from the group consisting of village wastewater.

  In the mixing step, the biological treatment agent may be added to a mixed solution containing the wastewater and the activated sludge. In the continuous activated sludge method, the biological treatment agent is returned to the returned sludge. Moreover, you may add to the liquid mixture containing the said waste water and the said activated sludge.

A method for adding the biological treatment agent to the mixed solution containing the waste water and activated sludge in the aeration tank is not particularly limited, but it is desirable to continuously or intermittently inject it into the aeration tank using a pump or the like. .
The addition amount of the biological treatment agent of the present invention is not particularly limited, but when the mixture (C) is not diluted, the biological treatment agent of the present invention is used. Is more preferable, 10 to 1000 ppm is more preferable, and 10 to 100 ppm is even more preferable.

When the biological treatment agent of the present invention is added to a mixed solution containing waste water and activated sludge in the aeration tank, the dissolved oxygen amount (concentration) of the mixed solution increases.
The dissolved oxygen amount (concentration) depends on the added amount of the biological treatment agent of the present invention. When the added concentration is increased, the dissolved oxygen amount (concentration) also increases.
Moreover, when the biological treatment agent of this invention is used, a state with a large dissolved oxygen amount (concentration) can be maintained for a long time.
That is, by adjusting the amount (concentration) to which the biological treatment agent is added, the dissolved oxygen amount (concentration) in the aeration mixture can be adjusted and maintained for a long time.
Although the measuring method of dissolved oxygen amount (concentration) is not specifically limited, For example, it can measure using a commercially available dissolved oxygen meter (DO meter) etc.
The amount (concentration) of dissolved oxygen is not particularly limited, but is preferably 5 mg / L or more, and more preferably 10 mg / L or more.

  The wastewater containing the organic compound is not particularly limited, but is not limited to food processing wastewater, beverage manufacturing wastewater, fermentation / brewing wastewater, pharmaceutical industry wastewater, chemical industry wastewater, textile industry wastewater, scouring / dyeing wastewater, fat and oil industry wastewater, sewage and At least one selected from the group consisting of settlement wastewater is preferred.

  Since the amount (concentration) of dissolved oxygen increases, the activity of aerobic microorganisms in the activated sludge increases without increasing the amount of aeration in the aeration tank, and the BOD is lower than when the biological treatment agent of the present invention is not added. Concentration can be achieved. That is, since the wastewater treatment method of the present invention does not require an increase in the amount of aeration, energy consumption is suppressed and the energy saving effect is excellent.

  According to the wastewater treatment method of the present invention, the surplus sludge generation amount is 10% by mass or more, preferably 15% by mass or more, more preferably 20% by mass or more, compared to the case where the wastewater treatment agent of the present invention is not added. More preferably, it can be reduced by 25% by mass or more. In order to treat surplus sludge, a large amount of energy is consumed. However, the wastewater treatment method of the present invention reduces the amount of surplus sludge production, thereby reducing the energy consumption for treating it, and the energy saving effect. Excellent.

[Waste water treatment equipment]
The present invention also provides a wastewater treatment facility for using the biological treatment agent of the present invention.

  A preferred wastewater treatment facility provided by the present invention is a wastewater treatment facility for treating wastewater containing an organic compound or the like by an activated sludge method, the wastewater, the activated sludge, and the biological treatment agent of the present invention. An aeration tank for aerating a liquid mixture containing the biological treatment agent, a drug storage tank for containing the biological treatment agent, and an input device for supplying the biological treatment agent from the chemical storage tank to the aeration tank; , Has.

  A preferred wastewater treatment facility provided by the present invention is a wastewater treatment facility for treating wastewater containing an organic compound or the like by an activated sludge method, the wastewater, the activated sludge, and the biological treatment agent of the present invention. An aeration tank for aeration of a mixed solution containing the activated sludge, a sedimentation tank for precipitating and separating activated sludge from the treated water flowing out of the aeration tank, or an activated sludge from the treated water flowing out of the aeration tank Separation membrane, a return sludge line for returning the return sludge extracted from the settling tank to the aeration tank, a drug storage tank for storing the biological treatment agent, and the biological treatment agent from the chemical storage tank And a charging device for charging into the return sludge line.

  The separation membrane is not particularly limited as long as it can separate treated water and activated sludge, which is usually used in a membrane separation activated sludge method (membrane activated sludge method), but is not limited to a microfiltration (MF) membrane or ultrafiltration. It is preferred to use a (UF) membrane. The separation membrane may be a flat membrane, tubular, hollow fiber, etc. Among these, tubular or hollow fiber is preferable. Furthermore, it is desirable to prevent fouling by using a conventionally known method such as vibrating the separation membrane.

  What is necessary is just to add a chemical | medical agent storage tank, an injection | throwing-in apparatus, and piping accompanying these to existing wastewater treatment facilities, and introduction is easy. The input device is not particularly limited as long as the biological treatment agent of the present invention can be input to the aeration tank or the return sludge line, and may be constituted by a pump and a pipe, for example.

[Pollution purification method by bioremediation]
The present invention provides a method for environmental purification by bioremediation of soil, groundwater or the like contaminated with an organic compound or the like.

  The environmental purification method by bioremediation of the present invention preferably includes a step of adding and mixing the biological treatment agent of the present invention to soil, groundwater or the like contaminated with the above organic compound or the like mixed with a bioremediation nutrient. .

  The environmental purification method by bioremediation of the present invention also includes the organism of the present invention in soil, groundwater, etc. contaminated with the above organic compound mixed with a microorganism having the ability to remove the above organic compound and the like and a bioremediation nutrient. What includes the process of adding a processing agent and mixing is also preferable.

  The contaminated soil, groundwater and the like may be subjected to the environmental purification method in situ, or the collected environmental samples may be subjected to the environmental purification method.

  The method of adding and mixing the biological treatment agent of the present invention to the contaminated soil, groundwater, etc. is not particularly limited, and can be performed by any method known to those skilled in the art.

  In the environmental purification method of the present invention, “organic compound and the like” refers to “organic compound and / or BOD component”.

  The organic compound is a compound having a carbon atom in the basic skeleton of the structure (carbon allotrope such as graphite and diamond; carbon monoxide, carbon dioxide; metal carbonate such as calcium carbonate; cyanic acid, metal cyanate and metal cyanate. And cyan compounds such as metal thiocyanates; other inorganic compounds having carbon atoms are excluded.)

  Specific examples of the organic compound include parathion, methyl parathion, methyl dimethone, EPN and other organic phosphorus compounds, polychlorinated biphenyl (PCB), dichloromethane, carbon tetrachloride, trichloroethylene, tetrachloroethylene, 1,2-dichloroethane, 1 , 1-dichloroethylene, cis-1,2-dichloroethylene, 1,1,1-trichloroethane, 1,1,2-trichloroethane, 1,3-dichloropropene, thiuram, simazine, thiobencarb, benzene, toluene, phenols, heavy Examples include oils such as quality oil.

  The BOD component is not particularly limited as long as it is measured as a BOD component by the method defined in Item 21 of JIS K 0102: 2008, and may be either an organic BOD component or an inorganic BOD component.

  Specific examples of the organic BOD component include saccharides, organic acids, starches, proteins, and lipids.

  Specific examples of the inorganic BOD component include sulfides, sulfite ions, cyanide compounds, and other reducing substances.

  The organic compound is preferably a volatile organic compound (VOC), such as benzene, 1,1-dichloroethylene, dichloromethane, cis-1,2-dichloroethylene, 1,1,1-trichloroethane, carbon tetrachloride, 1,2 -At least one selected from the group consisting of dichloroethane, trichloroethylene, 1,1,2-trichloroethane, 1,3-dichloropropane and tetrachloroethylene is more preferred.

  “Removing” means decomposing, precipitating (insolubilizing), absorbing (immobilizing), etc., but mainly means decomposing.

  A method for mixing the microorganism and the contaminated soil, groundwater, or the like is not particularly limited, and can be performed by any method known to those skilled in the art.

“Soil, groundwater, etc.” refers to soil, sediment, groundwater, river water, lake water or seawater, preferably soil, groundwater or lake water, more preferably soil or groundwater, more preferably Say soil.

  The bioremediation nutrient is not particularly limited as long as it contains a component that enhances the activity of microorganisms in the soil or microorganisms mixed in the soil, and commercially available products may be used.

  The method of mixing the bioremediation nutrient with the contaminated soil, groundwater, etc. is not particularly limited, and can be performed by any method known to those skilled in the art.

  EXAMPLES Hereinafter, although an Example demonstrates this invention more concretely, it describes clearly that the scope of the present invention is not limited to an Example.

[Manufacture of biological treatment agents]
1. Ingredients (1) Sodium hexametaphosphate (CAS # 10124-56-8)
(2) Glutathione oxidation type (CAS # 27025-41-8)
(3) Glycerol dehydrogenase (EC 1.1.1.6; 50 U / mg)
(4) Yeast lytic enzyme (5000 U / g; manufactured by Kanto Chemical Co., # 360954-N)
(5) Glycerin (CAS # 56-81-5)
(6) Sodium peroxodisulfate (CAS # 7775-27-1)
(7) Ethylenediaminetetraacetic acid (CAS # 60-00-4)
(8) Purified water (ion exchange water, electrical resistivity 1 MΩ · cm)

2. Production method (1) 4000 g of purified water, 1200 g of sodium hexametaphosphate, 20 mg of glutathione oxidation type, and 10 mg of glycerol dehydrogenase were mixed and stirred and mixed, and further in a refrigerator whose temperature was controlled at 0 to 5 ° C. Incubated for 7 days. This liquid mixture was designated as a liquid mixture (A) (see the column “Mixed liquid (A)” in Table 1).
(2) Thereafter, 100 g of yeast lytic enzyme and 1020 g of glycerin were added to 5200 g of the mixed solution (A), stirred and mixed, and further incubated for 5 days in an incubator controlled at 37 to 40 ° C. This liquid mixture was designated as a liquid mixture (B) (see the column “Mixed liquid (B)” in Table 1).
(3) Then, 800 g of the mixed solution (B), 2500 g of sodium peroxodisulfate, and 100 g of ethylenediaminetetraacetic acid are added to 16600 g of purified water, and the mixture is stirred and mixed. Further, at room temperature (20 ± 15 ° C.), Incubated for 7 days. This liquid mixture was designated as a liquid mixture (C) (see the column “Mixed liquid (C)” in Table 1).
(4) The liquid mixture (C) was used as a biological treatment agent as it was. In the following examples, the biological treatment agent thus produced is referred to as “biological treatment agent X”.

[Improvement of wastewater treatment by the activated sludge method]
The nature and amount of wastewater discharged from food manufacturing plants differ depending on the type of product and the manufacturing method. In addition, products such as sugar beet factories and beverage factories may change depending on the season, or the amount of wastewater and the amount of wastewater may change greatly due to fluctuations in production volume. However, most of the wastewater from food manufacturing plants is treated by the activated sludge process.
Food factory wastewater is generally characterized by containing high BOD carbohydrates, fats and proteins.

  This example compares the BOD concentration and MLSS concentration after wastewater treatment when the biological treatment agent of the present invention is used (addition example) and when it is not used (non-addition example).

1. Wastewater sample (1) Food manufacturing factory wastewater (BOD concentration: 780 mg / L)

2. Treatment agent, etc. (1) Biological treatment agent X (produced in Example 1)
(2) Aeration liquid (MLSS concentration: 5200 mg / L) of a wastewater treatment apparatus for food manufacturing plants

3. Test Method (1) 4000 mL of aeration liquid from a wastewater treatment apparatus for food production factory was put into a baffle type experimental apparatus, and aerated and stirred at 0.5 L / min for 1 hour using an air blower.
(2) A wastewater sample of 2000 mL was added to this aeration liquid, and a biological treatment agent X was added to a final concentration of 20 ppm (addition example), and a wastewater sample of 2000 mL was added. What was not added (non-addition example) was prepared.
(3) For each of the above addition example and non-addition example, the aeration stirring was further performed for a time.
(4) After the stirring was stopped and the treatment of the wastewater was completed, the biochemical oxygen consumption (BOD) was measured by the standard dilution method (item 21 of JIS K 0102: 2008). Further, the mixed suspension (MLSS) concentration was measured as a suspended substance by the method defined in Item 14.1 of JIS K 0102: 2008 (JIS B 9944: 1987 item 5.6 (1)).

4). Test results Table 2 below shows the measurement results of the BOD concentration and MLSS concentration.

4). Summary (1) BOD concentration The added example was 18 mg / L compared to 42 mg / L in the non-added example. That is, by adding the biological treatment agent X, the BOD concentration could be reduced by about 57%.
Since the BOD concentration can be lowered without increasing the stirring speed or adding aeration, the processing efficiency and the energy saving effect are excellent.
(2) MLSS concentration The addition example was 3820 mg / L compared with 5220 mg / L of the non-addition example of the biological treatment agent X. That is, the MLSS concentration could be reduced by about 27% by adding the biological treatment agent of the present invention.
By reducing the MLSS concentration, the amount of surplus sludge generated can be reduced, and the energy consumption and cost for surplus sludge treatment can be reduced. Further, in the membrane separation activated sludge method using a hollow fiber membrane or the like, the processing efficiency can be improved by reducing the clogging of the filtration membrane due to the suspended activated sludge.

[Relationship between dissolved oxygen amount and biological treatment agent addition amount]
1. Materials and methods (1) Sample used Food factory wastewater aeration tank mixture (MLSS concentration: 4500mg / L)
Chemical factory wastewater aeration tank mixture (MLSS concentration: 3350mg / L)
(2) Test method Take 1000 mL of the sample mixture in a beaker, and stir at 300 rpm using a magnetic stirrer, and the biological treatment agent (biological treatment agent X) produced in Example 1 has the concentration shown in Table 4. The mixture was stirred for 3 minutes, and the dissolved oxygen amount was measured using a dissolved oxygen meter. At this time, there was no change in the water temperature.
2. Results and Discussion Table 4 shows the measurement results of the dissolved oxygen amount for each of the food factory wastewater and the chemical factory wastewater.
When the addition amount of the biological treatment agent X is increased, the dissolved oxygen amount increases depending on the addition amount, and the dissolved oxygen amount obtained varies depending on the type of waste water.

[Changes in dissolved oxygen over time]
1. Materials and methods (1) Wastewater samples Food factory wastewater (BOD concentration: 480 mg / L)
(2) Treatment agent etc. Food factory aeration tank mixture (MLSS concentration: 4300mg / L)
Biological treatment agent X (produced in Example 1)
2. Test Method (1) 60 L of a food factory aeration tank mixed solution was put in a baffle type experimental apparatus, and aerated and stirred at 4.0 L / min for 2 hours using an air blower.
The biological treatment agent X (final concentration 20 ppm) and the wastewater sample 20L were added thereto, and stirring (referred to as “non-aeration stirring” in this example) was performed for 8 hours without aeration with a blower.
(2) 1 hour and 2 hours after the start of aeration stirring, at the start of non-aeration stirring (0 hour), 2 hours after the start of non-aeration stirring, and thereafter every 8 hours until the start of 8 hours. Using this, the amount of dissolved oxygen was measured.
2. Results and Discussion Table 4 shows the measured values of dissolved oxygen content.
The dissolved oxygen amount was 2.45 mg / L after 2 hours of aeration stirring and 12.2 mg / L after 0 hours of non-aeration stirring. Was maintained. It turns out that the amount of dissolved oxygen increased rapidly by adding the biological treatment agent X.
The MLSS concentration after 8 hours was 3100 mg / L, and a decrease of 25% or more was observed.

[Reduction of bioremediation processing period]
Trichlorethylene (TCE) has a track record of being used as a stain for dry cleaning and degreasing cleaners for metals, machines, etc., but it is excellent as a cleaning agent and solvent, but is stable even when discharged into the environment. Since soil adsorption is weak, it can easily permeate into groundwater and contaminate groundwater, and may remain for a long time.

  In this example, in the bioremediation treatment of soil contaminated with trichlorethylene, when the biological treatment agent of the present invention was added, the environmental standard related to soil contamination (0.03 mg per liter of test solution) compared to the case where it was not added. The following: Indicates that the period required for processing to the extent that satisfies the Environment Agency Notification No. 46 (1991) can be shortened.

1. Soil samples (1) Soil samples taken from the site of a wool processing factory Treatment agent, etc. (1) Biological treatment agent X (produced in Example 1)
3. Test method (1) Place 3000 mg of a soil sample to which a bioremediation nutrient has been added into a 5000 mL beaker, and add biological treatment agent X to a concentration of 100 ppm (addition example 1) or 300 ppm (addition example 2). Alternatively, stirring was started at room temperature without adding the biological treatment agent X (non-addition example).
(2) Specimens were collected at the time of starting stirring, and on the 5th, 10th, 15th, and 20th days after starting stirring, with the time of starting stirring as the standard (0 days).
(3) For the collected samples, a test solution was prepared in accordance with a designated test solution preparation method (Attached Table No. 46 of Environment Agency Notification No. 46, August 1991) as follows.
3.1) Handling of collected soil Since trichlorethylene has high volatility, the collected specimen was stored in a glass container that can be sealed or a container that does not adsorb the substance to be measured so that no void remains. As a rule, the test was carried out immediately after soil collection. If the test could not be performed immediately, it was stored in a cool dark place at 4 ° C. or lower, and the test was performed as quickly as possible.
3.2) Preparation of samples Excludes small pebbles, wood chips, etc. with a particle size of more than 5mm from the collected soil.
3.3) Preparation of sample solution Sample (unit: g) and solvent (hydrochloric acid is added to pure water, and hydrogen ion concentration index is 5.8 or more and 6.3 or less in a conical flask with a screw cap which has been previously stirred. (Unit: ml) was taken so that the ratio by weight to volume was 10%, and the cap was quickly sealed. At this time, the liquid mixture was set to 500 ml or more, and the head space of the conical flask with a screw mouth for the liquid mixture was made as small as possible.
3.4) Elution The prepared sample solution was kept at room temperature (approximately 20 ° C.) and normal pressure (approximately 1 atm) and stirred continuously with a magnetic stirrer for 4 hours.
3.5) Preparation of test solution The sample solution obtained by performing the operations of 3.1) to 3.4) is allowed to stand for about 10 to 30 minutes, and then gently sucked into a glass syringe to obtain a pore size of 0. Connect a filter paper holder equipped with a 45 μm membrane filter (compatible with the diameter of the membrane filter to be used and made of stainless steel or equivalent material) and push the inner cylinder of the syringe barrel, Several ml was discharged, and then the filtrate was collected into a test tube with a stopper, and the amount required for quantification was accurately measured, and this was used as a test solution.
(4) Purified trap-gas chromatograph mass spectrometry (JIS K 0125: 1995 5.1) according to the designated measuring method (Attached Table No. 46 of the Environment Agency Notification of August 1991) for the prepared test solution The amount of trichlorethylene (unit: ng) in the test solution was measured. From the applied sample amount (unit: μL) and the measured value (ng), the amount of trichlorethylene (unit: mg) per liter of sample, that is, the concentration of trichlorethylene in the test solution (unit: mg / L) was calculated.
3. Test Results Table 5 below shows the trichlorethylene concentration (mg / L) in the test solution.

4). Summary In the non-addition example in which the biological treatment agent of the present invention was not added, the soil environment standard (0.03 mg / L) could not be achieved even at the time of 20 days after the start of the bioremediation treatment. It was.
In contrast, in addition example 1 (addition of 100 ppm) and addition example 2 to which the biological treatment agent of the present invention was added, 15 days (addition example 2) or 20 days (addition example 1) after the start of the bioremediation treatment. At this point, the soil environmental standard (0.03 mg / L) is significantly below, and it is expected that the treatment period can be greatly shortened compared to the non-added example. Moreover, since the measured value is less than 0.003 mg / L, regulations will be strengthened in the future. For example, even when the reference value becomes 0.01 mg / L, it is possible to cope with it sufficiently.
From the above, it was found that the treatment period can be greatly shortened when the biological treatment agent of the present invention is used for bioremediation treatment of soil.

  In this example, the amount of sodium hexametaphosphate, glutathione oxidation type, glycerol dehydrogenase, yeast lytic enzyme, glycerin, sodium peroxodisulfate or ethylenediaminetetraacetic acid in the method for producing the chemical treating agent X of Example 1 above was used. About the changed example, comprehensive performance was evaluated from the BOD concentration reduction rate and the MLSS concentration reduction rate.

1. Evaluation Criteria (1) Temporary Evaluation Based on BOD Concentration Reduction Rate The larger the BOD concentration reduction rate, the better. A reduction rate of 75% or more is evaluated as “A” as Excellent, and a reduction rate of 50% or more and less than 75% is evaluated as “B” as Good, and a reduction rate of 40% or more and 50%. Less than was evaluated as "C" as Fair, and less than 40% was evaluated as "D" as Poor.

(2) Temporary evaluation based on MLSS concentration reduction rate The larger the MLSS concentration reduction rate, the better. A reduction rate of 15% or more is evaluated as “A” as Excellent, and a reduction rate of 6% or more and less than 15% is evaluated as “B” as Good, and a reduction rate of 3% or more and 6% Less than was rated as “C” as Fair, and less than 3% was rated as “D” as Poor.

(3) Comprehensive evaluation When the evaluation of BOD and the evaluation of MLSS are the same, with the evaluation, when different, it was set as the comprehensive evaluation of the biological treatment liquid with the lower evaluation.

2. Production of biological treatment liquid and measurement of BOD concentration reduction rate and MLSS concentration reduction rate (1) Example of changing the amount of sodium hexametaphosphate <Manufacture of biological treatment agent>
It manufactured according to the manufacturing method of the chemical processing agent X of Example 1 except the point which changed the compounding quantity of hexametaphosphoric acid as described in following Table 6 among the compounding quantities of each component shown in Table 1 of Example 1. .

<Measurement of BOD concentration reduction rate and MLSS concentration reduction rate>
The chemical treatment agent produced as described above is a mixture of dairy production wastewater and activated sludge (dairy production aeration mixture) (BOD concentration 4100 mg / L, MLSS concentration 2800-2860 mg / L, SS concentration 120 mg / L, (T-P4.2 mg / L) was added to a concentration of 30 ppm, and then aeration was continued for 120 minutes. The blank is an example in which no biological treatment agent was added.
The BOD concentration and MLSS concentration before and after aeration were measured. The BOD concentration was measured by the method described in Item 14.2 of JIS K 0102: 2008, and the MLSS concentration was measured by the method described in Item 21 of JIS K 0102: 2008.
Based on the BOD concentration and MLSS concentration before the start of aeration, the decrease rate of the BOD concentration and MLSS concentration after aeration was determined.

<Evaluation>
The performance of each biological treatment agent was evaluated according to the above criteria.

(2) Example of changing the blending amount of glutathione oxidation type Among the blending amounts of each component shown in Table 1 of Example 1, except that the blending amount of glutathione oxidation type was changed as described in Table 7 below, The chemical treatment agent X was produced according to the production method of Example 1.

<Measurement of BOD concentration reduction rate and MLSS concentration reduction rate>
The chemical treatment agent produced as described above is a mixture of dairy production wastewater and activated sludge (dairy production aeration mixture) (BOD concentration 4100 mg / L, MLSS concentration 2800-2860 mg / L, SS concentration 120 mg / L, (T-P4.2 mg / L) was added to a concentration of 30 ppm, and then aeration was continued for 120 minutes. The blank is an example in which no biological treatment agent was added.
The BOD concentration and MLSS concentration before and after aeration were measured. The BOD concentration was measured by the method described in Item 14.2 of JIS K 0102: 2008, and the MLSS concentration was measured by the method described in Item 21 of JIS K 0102: 2008.
Based on the BOD concentration and MLSS concentration before the start of aeration, the decrease rate of the BOD concentration and MLSS concentration after aeration was determined.

<Evaluation>
The performance of each biological treatment agent was evaluated according to the above criteria.

(3) Example of changing the blending amount of glycerol dehydrogenase Among the blending amounts of each component shown in Table 1 of Example 1, the blending amount of glycerol dehydrogenase was changed as described in Table 8 below. Except for this, the chemical treating agent X of Example 1 was produced according to the production method.

<Measurement of BOD concentration reduction rate and MLSS concentration reduction rate>
The chemical treatment agent produced as described above is a mixture of dairy production wastewater and activated sludge (dairy production aeration mixture) (BOD concentration 4100 mg / L, MLSS concentration 2800-2860 mg / L, SS concentration 120 mg / L, (T-P4.2 mg / L) was added to a concentration of 30 ppm, and then aeration was continued for 120 minutes. The blank is an example in which no biological treatment agent was added.
The BOD concentration and MLSS concentration before and after aeration were measured. The BOD concentration was measured by the method described in Item 14.2 of JIS K 0102: 2008, and the MLSS concentration was measured by the method described in Item 21 of JIS K 0102: 2008.
Based on the BOD concentration and MLSS concentration before the start of aeration, the decrease rate of the BOD concentration and MLSS concentration after aeration was determined.

<Evaluation>
The performance of each biological treatment agent was evaluated according to the above criteria.

(4) Example of changing the amount of yeast lytic enzyme The amount of yeast lytic enzyme in the amount of each component shown in Table 1 of Example 1 was changed as described in Table 9 below. The chemical treatment agent X was produced according to the production method of Example 1.

<Measurement of BOD concentration reduction rate and MLSS concentration reduction rate>
The chemical treatment agent produced as described above is a mixture of dairy production wastewater and activated sludge (dairy production aeration mixture) (BOD concentration 4100 mg / L, MLSS concentration 2800-2860 mg / L, SS concentration 120 mg / L, (T-P4.2 mg / L) was added to a concentration of 30 ppm, and then aeration was continued for 120 minutes. The blank is an example in which no biological treatment agent was added.
The BOD concentration and MLSS concentration before and after aeration were measured. The BOD concentration was measured by the method described in Item 14.2 of JIS K 0102: 2008, and the MLSS concentration was measured by the method described in Item 21 of JIS K 0102: 2008.
Based on the BOD concentration and MLSS concentration before the start of aeration, the decrease rate of the BOD concentration and MLSS concentration after aeration was determined.

<Evaluation>
The performance of each biological treatment agent was evaluated according to the above criteria.

(5) Example of changing the blending amount of glycerin <Manufacture of biological treatment agent>
It manufactured according to the manufacturing method of the chemical processing agent X of Example 1 except the point which changed the compounding quantity of glycerol among the compounding quantities of each component shown in Table 1 of Example 1 as described in the following Table 10.

<Measurement of BOD concentration reduction rate and MLSS concentration reduction rate>
The chemical treatment agent produced as described above is a mixture of dairy production wastewater and activated sludge (dairy production aeration mixture) (BOD concentration 4100 mg / L, MLSS concentration 2800-2860 mg / L, SS concentration 120 mg / L, (T-P4.2 mg / L) was added to a concentration of 30 ppm, and then aeration was continued for 120 minutes. The blank is an example in which no biological treatment agent was added.
The BOD concentration and MLSS concentration before and after aeration were measured. The BOD concentration was measured by the method described in Item 14.2 of JIS K 0102: 2008, and the MLSS concentration was measured by the method described in Item 21 of JIS K 0102: 2008.
Based on the BOD concentration and MLSS concentration before the start of aeration, the decrease rate of the BOD concentration and MLSS concentration after aeration was determined.

<Evaluation>
The performance of each biological treatment agent was evaluated according to the above criteria.

(6) Example of changing the amount of sodium peroxodisulfate <Manufacture of biological treatment agent>
According to the method for producing the chemical treating agent X of Example 1, except that the amount of sodium peroxodisulfate was changed as described in Table 11 below among the amounts of each component shown in Table 1 of Example 1. Manufactured.

<Measurement of BOD concentration reduction rate and MLSS concentration reduction rate>
The chemical treatment agent produced as described above was mixed with food production wastewater and activated sludge (food production aeration mixture) (BOD concentration 5200 mg / L, MLSS concentration 4200-4300 mg / L, SS concentration 160 mg / L, COD 3200 mgT. -P4.2 mg / L) was added to a concentration of 30 ppm, and then aeration was continued for 200 minutes. The blank is an example in which no biological treatment agent was added.
The BOD concentration and MLSS concentration before and after aeration were measured. The BOD concentration was measured by the method described in Item 14.2 of JIS K 0102: 2008, and the MLSS concentration was measured by the method described in Item 21 of JIS K 0102: 2008.
Based on the BOD concentration and MLSS concentration before the start of aeration, the decrease rate of the BOD concentration and MLSS concentration after aeration was determined.

<Evaluation>
The performance of each biological treatment agent was evaluated according to the above criteria.

(7) Example of changing blending amount of ethylenediaminetetraacetic acid <Manufacture of biological treatment agent>
Manufactured according to the method for producing chemical treating agent X of Example 1 except that the blending amount of ethylenediaminetetraacetic acid in the blending amounts of each component shown in Table 1 of Example 1 was changed as described in Table 12 below. did.

<Measurement of BOD concentration reduction rate and MLSS concentration reduction rate>
The chemical treatment agent produced as described above was mixed with food production wastewater and activated sludge (food production aeration mixture) (BOD concentration 5200 mg / L, MLSS concentration 4200-4300 mg / L, SS concentration 160 mg / L, COD 3200 mgT. -P4.2 mg / L) was added to a concentration of 30 ppm, and then aeration was continued for 200 minutes. The blank is an example in which no biological treatment agent was added.
The BOD concentration and MLSS concentration before and after aeration were measured. The BOD concentration was measured by the method described in Item 14.2 of JIS K 0102: 2008, and the MLSS concentration was measured by the method described in Item 21 of JIS K 0102: 2008.
Based on the BOD concentration and MLSS concentration before the start of aeration, the decrease rate of the BOD concentration and MLSS concentration after aeration was determined.

<Evaluation>
The performance of each biological treatment agent was evaluated according to the above criteria.

3. Summary As can be seen from the results shown in Tables 1 to 12, all of the biological treatment agents of the present invention have an overall evaluation C or higher, and are useful for wastewater treatment.

Claims (14)

It corresponds to 4000 parts by mass of water, 800 to 1600 parts by mass of sodium hexametaphosphate, 0.001 to 0.100 parts by mass of glutathione oxidation type, and 0.001 to 0.100 parts by mass of 50 U / mg glycerol dehydrogenase. A unit number of glycerol dehydrogenase was mixed and further incubated for 5 days or more to prepare a mixed solution (A). The prepared mixed solution (A) 5200 parts by mass and 5000 U / g yeast lytic enzyme 50 to 150 A yeast lytic enzyme having the number of units corresponding to parts by mass and 800 to 1500 parts by mass of glycerin are mixed and further incubated for 3 days or more to prepare a mixed solution (B), and 800 parts by mass of the prepared mixed solution (B). And moles corresponding to 1000 to 4000 parts by mass of sodium peroxodisulfate and 10 to 200 parts by mass of ethylenediaminetetraacetic acid Of ethylenediaminetetraacetic acid or a water-soluble salt thereof and the mixture (B), sodium peroxodisulfate and an amount of water capable of dissolving the ethylenediaminetetraacetic acid or a water-soluble salt thereof, and further incubated for 5 days or more. A biological treatment agent obtained by preparing a mixed solution (C) and diluting the prepared mixed solution (C) as it is or with water . It corresponds to 4000 parts by mass of water, 800 to 1600 parts by mass of sodium hexametaphosphate, 0.001 to 0.100 parts by mass of glutathione oxidation type, and 0.001 to 0.100 parts by mass of 50 U / mg glycerol dehydrogenase. A step of mixing a unit number of glycerol dehydrogenase and further incubating for 5 days or more to prepare a mixed solution (A),
5200 parts by mass of the prepared mixed liquid (A) , yeast lytic enzyme having a unit number corresponding to 50 to 150 parts by mass of 5000 U / g yeast lytic enzyme, and 800 to 1500 parts by mass of glycerin are mixed, and further 3 days or more Incubating to prepare a mixture (B); and
And prepared mixture liquid (B) 800 parts by weight, and Bae Ruokiso disodium sulphate 1000-4000 parts by weight, and the number of moles of ethylene diamine tetraacetic acid or its water-soluble salts corresponding to ethylenediaminetetraacetic acid 10 to 200 parts by weight, the mixing liquid (B), comprising the step of the peroxodisulfate water in an amount mixed capable sodium sulfate and dissolve the ethylenediaminetetraacetic acid or its water-soluble salts, to prepare further 5 days or more incubated with mixture (C), live A method for producing a material treatment agent. A wastewater treatment method using an activated sludge method for wastewater containing an organic compound and / or a BOD component,
A wastewater treatment method, comprising: a mixing step of mixing the wastewater, activated sludge, and the biological treatment agent according to claim 1; and an aeration step of aerating the mixed solution obtained in the mixing step.   The wastewater treatment method according to claim 3, wherein in the mixing step, the biological treatment agent is added to a mixed liquid containing the wastewater and the activated sludge.   The wastewater treatment method according to claim 3, wherein, in the mixing step, the biological treatment agent is added to a mixed solution containing the wastewater and the activated sludge together with the return sludge.   The wastewater treatment method according to claim 4 or 5, wherein when adding the biological treatment agent, the amount of dissolved oxygen in the mixed solution is measured, and the addition amount of the biological treatment agent is adjusted based on the amount of dissolved oxygen. .   The wastewater is at least selected from the group consisting of food processing wastewater, beverage manufacturing wastewater, fermentation / brewing wastewater, pharmaceutical industry wastewater, chemical industry wastewater, textile industry wastewater, scouring / dyeing wastewater, fat and oil industry wastewater, sewage and settlement wastewater. The wastewater treatment method according to any one of claims 3 to 6, wherein the number is one. A wastewater treatment facility for treating wastewater by an activated sludge method containing wastewater containing organic compounds and / or BOD components,
An aeration tank for aeration of a mixed liquid containing the wastewater, activated sludge, and the biological treatment agent according to claim 1;
A chemical reservoir for storing the biological treatment agent;
An input device for supplying the biological treatment agent from the drug storage tank to the aeration tank;
Wastewater treatment facility comprising. A wastewater treatment facility for treating wastewater by an activated sludge method containing wastewater containing organic compounds and / or BOD components,
An aeration tank for aeration of a mixed liquid containing the wastewater, activated sludge, and the biological treatment agent according to claim 1;
A settling tank for precipitating and separating activated sludge from the treated water flowing out of the aeration tank or a separation membrane for separating activated sludge from the treated water flowing out of the aeration tank;
A return sludge line for returning the activated sludge extracted from the settling tank or the activated sludge separated by the separation membrane to the aeration tank;
A chemical reservoir for storing the biological treatment agent;
An input device for supplying the biological treatment agent from the chemical storage tank to the return sludge line;
Wastewater treatment facility comprising.   The wastewater is at least selected from the group consisting of food processing wastewater, beverage manufacturing wastewater, fermentation / brewing wastewater, pharmaceutical industry wastewater, chemical industry wastewater, textile industry wastewater, scouring / dyeing wastewater, fat and oil industry wastewater, sewage and settlement wastewater. The wastewater treatment facility according to claim 8 or 9, wherein the number is one. A method for environmental purification by bioremediation of soil, sediment, groundwater, river water, lake water or seawater contaminated with organic compounds and / or BOD components,
An environmental purification method comprising a step of adding and mixing the biological treatment agent according to claim 1 to the soil, sediment, groundwater, river water, lake water, or seawater mixed with a bioremediation nutrient. A method for environmental purification by bioremediation of soil, sediment, groundwater, river water, lake water or seawater contaminated with organic compounds and / or BOD components,
The biological treatment agent according to claim 1 is added to the soil, sediment, groundwater, river water, lake water, or seawater mixed with a microorganism having the ability to remove the organic compound and / or BOD component and a bioremediation nutrient. An environmental purification method comprising the steps of adding and mixing.   The environmental purification method according to claim 11 or 12, wherein the organic compound and / or BOD component is a volatile organic compound (VOC).   The volatile organic compound is benzene, 1,1-dichloroethylene, dichloromethane, cis-1,2-dichloroethylene, 1,1,1-trichloroethane, carbon tetrachloride, 1,2-dichloroethane, trichloroethylene, 1,1,2 The environmental purification method according to claim 13, which is at least one selected from the group consisting of -trichloroethane, 1,3-dichloropropane, and tetrachloroethylene.